ArticlePDF AvailableLiterature Review

Abstract and Figures

Since time immemorial, phages—the viral parasites of bacteria—have been protecting Earth’s biosphere against bacterial overgrowth. Today, phages could help address the antibiotic resistance crisis that affects all of society. The greatest hurdle to the introduction of phage therapy in Western medicine is the lack of an appropriate legal and regulatory framework. Belgium is now implementing a pragmatic phage therapy framework that centers on the magistral preparation (compounding pharmacy in the US) of tailor-made phage medicines.
This content is subject to copyright.
viruses
Communication
The Magistral Phage
Jean-Paul Pirnay 1, *, Gilbert Verbeken 1, Pieter-Jan Ceyssens 2, Isabelle Huys 3, Daniel De Vos 1,
Charlotte Ameloot 4and Alan Fauconnier 4,5
1Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Bruynstraat 1,
1120 Brussel, Belgium; gilbert.verbeken@mil.be (G.V.); danielmarie.devos@mil.be (D.D.V.)
2Bacterial Diseases, Unit Antibiotic Resistance, Scientific Institute of Public Health, Rue Engelandstraat 642,
1180 Brussel, Belgium; pieterjan.ceyssens@wiv-isp.be
3Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, O&N2, Herestraat 49, Box 521,
3000 Leuven, Belgium; isabelle.huys@pharm.kuleuven.be
4Federal Agency for Medicines and Health Products, Place Victor Horta 40/40, 1060 Brussels, Belgium;
charlotte.ameloot@fagg-afmps.be (C.A.); alan.fauconnier@invivo.be (A.F.)
5Culture In Vivo ASBL, rue du Progrès, 4, boîte 7, 1400 Nivelles, Belgium
*Correspondence: jean-paul.pirnay@mil.be; Tel.: +32-2-264-4844
Received: 15 January 2018; Accepted: 3 February 2018; Published: 6 February 2018
Abstract:
Since time immemorial, phages—the viral parasites of bacteria—have been protecting
Earth’s biosphere against bacterial overgrowth. Today, phages could help address the antibiotic
resistance crisis that affects all of society. The greatest hurdle to the introduction of phage therapy
in Western medicine is the lack of an appropriate legal and regulatory framework. Belgium is now
implementing a pragmatic phage therapy framework that centers on the magistral preparation
(compounding pharmacy in the US) of tailor-made phage medicines.
Keywords:
antibiotic; antimicrobial resistance; magistral preparation; compounding pharmacy;
phage therapy; regulatory framework; personalized medicine
1. The Age of the Superbug
On 21 September 2016, the UN General Assembly convened a meeting on antimicrobial resistance
(AMR) at the UN headquarters in New York. It was only the fourth time the General Assembly
addressed a health emergency. This high-level meeting resulted in a UN resolution focused on
combatting the AMR health threat. World leaders acknowledged that global AMR poses a fundamental
long-term threat to human health, the production of food, and sustainable development. Based on
scenarios of rising drug resistance for six pathogens, experts estimated that by 2050 the burden of
AMR could rise to 10 million people dying every year and an economic cost of $100 trillion [1].
Commercial antibiotics that are currently used in public health, animal, food, agriculture and
aquaculture sectors, are immutable chemicals that are based on natural antibiotics produced by soil
bacteria or fungi to—depending on their concentration—either combat competitors, communicate with
other organisms, or act as pleiotropic effectors of metabolic pathways. It was, therefore, to be expected
that bacteria would be extremely proficient at evolving resistance to such antibiotics, especially when
these are excessively and often unnecessarily used. Selective pressures imposed by humans have
resulted in the emergence of “superbugs”, or bacteria that are resistant to virtually all commercial
antibiotics. Experts fear that society could return to a pre-antibiotic era, when simple infections could
wipe out entire populations and surgical interventions were life threatening. Today, it seems that all
“easy” antibiotics have been exploited and industry has been reluctant to put new efforts into the
discovery and development of new classes of antibiotics. These are expensive to develop and are
bound to offer a poor return on investment as they are only taken for a short period and their use is
likely to be restricted in the future.
Viruses 2018,10, 64; doi:10.3390/v10020064 www.mdpi.com/journal/viruses
Viruses 2018,10, 64 2 of 7
Therefore, the UN committed to work at national, regional and global levels to support the
development of new antimicrobial agents and therapies [2].
2. Phage Therapy
One of the promising “new” treatments that is increasingly highlighted—inter alia during the
recent UN General Assembly—is phage therapy, the therapeutic use of bacteriophages (phages in
short)—the viruses of bacteria—to treat bacterial infections [
3
]. Since time immemorial, phages
control their hosts, the bacteria, on our planet. When discovered in the early twentieth century, they
were immediately applied in medicine. It soon appeared that phages are exquisitely host-specific.
Most phages can only lyse a subset of a bacterial species. Physicians must thus first know which
bacteria cause the infections before they can treat the patients.
As could be expected, it was shown that bacteria could also evolve to evade phage infection,
even when potent phages are applied simultaneously [
4
]. However, the main advantage of phages
over antibiotics is their ability to mutate at least as fast as their hosts, enabling them to evolve new
infectivity and thus regain the “upper hand” over bacteria. Bacteria and phage are thus involved in a
continuous arms race of co-evolving infectivity and defense mechanisms.
The advent of broad-spectrum antibiotics, which target a wide range of bacterial infections and
could thus be used empirically, heralded the decline of phage therapy in the Western world. The success
stories of the many phage applications in the past, mainly on the east side of the Iron Curtain, where
phage therapy remained an established treatment, together with the increasing number of virtually
untreatable bacterial infections, has created a growing demand for phage therapy. Some successful
intravenous applications of phages to treat terminally ill patients in the Western world have recently
been published in the scientific literature [5,6].
The Promise of the Phage Therapy Medicinal Product
At their reintroduction in the Western world, phage preparations were classified as medicinal
products (European Union) or drugs (US), based on the literal implementation of definitions. Namely,
any substance presented as having properties for treating or preventing disease in human beings is
considered to be a medicinal product or a drug. As a result, a large body of costly and time-consuming
requirements and procedures for manufacturing and for obtaining marketing authorization for
medicinal products (drugs in the US) for human use were imposed on phage therapy medicinal
products (PTMPs).
On the one hand, it turns out that the established pharmaceutical industry is not interested in
PTMPs, mainly because of limitations in intellectual property protection of natural entities such as
genes or phages and because of phage specificity and bacterial resistance issues, which compromise
widespread and long-term use of immutable pre-defined PTMPs. On the other hand, it is becoming
clear that medicinal product provisions, which were originally developed to cater for widely used
and mass-produced chemical molecules such as aspirin and antibiotics, are not compatible with
sustainable (non-empirical) or customized phage therapy approaches in which phages need to be
selected and produced ad hoc [
7
]. Pre-defined PTMPs, could make it through the medicinal product
funnel, but such preparations are less flexible to deal with changes in the incidences of infecting
bacterial species in certain settings or geographical areas, or with the emergence of mutated bacterial
strains. The long-term use of immutable PTMPs is also bound to elicit considerable bacterial phage
resistance, although not much is known about the rate at which this would occur in clinical settings.
Overall, the efficacy of PTMPs is likely to decrease over time and they would need to be regularly
adapted and re-approved for use.
Some of these issues crystallized during PhagoBurn (www.phagoburn.eu), the first major trial
under modern medicinal product regulatory standards in the European Union [
8
]. Cocktails of 12 and
13 phages were needed to ensure a certain activity against a collection of Pseudomonas aeruginosa and
E. coli isolates, respectively. Manufacturing of one batch of the investigational products ended up taking
Viruses 2018,10, 64 3 of 7
20 months and the largest part of the study budget. In addition, phage specificity issues hampered
the recruitment of patients. Because each of the two study products, which couldn’t be applied
simultaneously, targeted only one of the multiple bacterial species that are known to (simultaneously)
infect or colonize burn wounds, physicians were reluctant to include patients [
8
]. Regardless of the
final clinical outcome of PhagoBurn, the preliminary phase of the study showed at least that dedicated
and realistic production and documentation requirements are urgently needed to enable the timely
supply of secure phage preparations. This would enable clinicians to conduct the desperately needed
safety and efficacy studies and to deal with urgent individual or local infection issues or public health
threats (e.g., the 2011 E. coli O104:H4 outbreak in Germany).
Meanwhile, sporadic phage applications are carried out in the West, often under the umbrella of
Article 37 (Unproven Interventions in Clinical Practice) of the Declaration of Helsinki (www.wma.net).
In addition, several European and US patients suffering from chronic, extremely resistant or difficult
to treat bacterial infections are known to have travelled to a phage therapy center in Tbilisi, Georgia
(www.eliavaphagetherapy.com,www.phagetherapycenter.com), for treatment.
3. Enter the Magistral Phage
On 5 July 2016, during a meeting of the Belgian Chamber of Representatives and in response to
two parliamentary questions related to the implementation of phage therapy [
9
], the Belgian Minister
of Social Affairs and Public Health acknowledged that phage therapy has no specific regulation in
Europe and that there is a consensus that phage preparations are medicinal products. However,
according to the Minister it is difficult to determine whether we should deal with industrially-prepared
medicinal products or rather with magistral preparations, the former being subject to constraints
related to their production and marketing authorization, unlike the latter.
3.1. Magistral Preparations
In European and Belgian law, the notion of a magistral preparation (compounded prescription
drug product in the US) is defined as “any medicinal product prepared in a pharmacy in accordance
with a medical prescription for an individual patient” (Article 3 of Directive 2001/83 and Article 6
quater, § 3 of the Law of 25 March 1964). Magistral preparations are mixed from their constituent
ingredients by a pharmacist (or at least under his/her supervision), for a given patient according to a
prescription by a physician and following the technical and scientific standards of the pharmaceutical
art. The magistral formula is a practical way for a medical doctor to personalize patient treatments
to specific needs and to make medications available that do not exist commercially. Some medicines,
such as natural hormone combination products and allergens, are not produced by commercial
manufacturers because they lack patent protection and hence return on investment for pharmaceutical
companies, but are actually delivered as magistral preparations. Owing to the emergence of innovative
medicines for rare diseases or for personalized therapies, magistral preparations are increasingly
in demand.
3.2. The Belgian Magistral Phage Medicine Strategy
The Community code leaves the door open for some flexibility to implement certain national
solutions relating to medicines for human use [
10
]. As such, the Belgian Minister of Public Health asked
the Federal Agency for Medicines and Health Products (FAMHP, the Belgian competent authority
for medicines) to help set up a national strategy for magistral phage medicines. In general, active
ingredients of magistral preparations must meet the requirements of the European Pharmacopoeia,
of the Belgian Pharmacopoeia or of an official pharmacopoeia [
10
]. If no such document exists, then the
active ingredients must be authorized by the Minister of Public Health, following a favorable opinion
of the national Pharmacopoeia Commission [
10
]. In addition, non-authorized ingredients may also
be used in magistral preparations, providing that they are accompanied by a certificate of analysis
issued by a Belgian Approved Laboratory [
10
]. The so-called “Belgian Approved Laboratories” are
Viruses 2018,10, 64 4 of 7
quality control laboratories which are granted an accreditation by the Belgian regulatory authorities.
This status allows them to perform the batch release testing of medicinal products. This national
accreditation is equivalent to—and gradually replaced by—the GMP certification for the batch release
testing of medicinal products. Belgian Approved Laboratories can be either private (e.g., subcontractor
of the pharmaceutical industry) or partially or entirely public (e.g., academic laboratories and scientific
institutes). Some of them belong to the European Official Medicines Control Laboratories (OMCL)
network, which is made up of independent public laboratories that have been appointed by their
respective national authority.
The option of the “non-authorized ingredient” was chosen in this case because of the enormous
variety of phages that could qualify as active ingredients and should then, each individually, obtain
an authorization issued by the Minister of Public Health [
9
]. The Scientific Institute of Public Health
was identified as a suitable Belgian Approved Laboratory for issuing valid certificates of analysis
for batches of phage active ingredients. Although the standard procedure for unauthorized active
ingredients only involves the medical doctor, his patient, the manufacturer of the active substances,
the approved laboratory and the pharmacist, it was decided—in joint consultation and because of the
innovative and very specific character of phage therapy—to involve the FAMHP in the elaboration of
a Belgian magistral phage medicine procedure.
In practice, and to consolidate the opening left by the Minister of Public Health, a formal question
and answer session was initiated between the military hospital and the FAMHP within the context
of the existing national Scientific-Technical Advice (STA) procedure. On 26 October 2016, it was
formally agreed that natural phages whose derivative finished products are not fully compliant with
the requirements relating to medicinal products for human use (Directive 2001/83), and for which
there is no monograph in an official pharmacopoeia, can be processed by a pharmacist as active
pharmaceutical ingredients (APIs) in magistral preparations, providing compliance to a number of
logical provisions:
Phages should be delivered in the form of a magistral preparation to a specific (nominal) patient.
Magistral preparations should always be delivered under the direct responsibility of a medical
doctor and a pharmacist.
The relevant characteristics and qualities of the phage APIs should be defined in an internal
monograph (prepared by the supplier).
Before the pharmacist can use the unlicensed material, he/she must ascertain—based on
certificates of analysis issued by a Belgian Approved Laboratory—that the raw materials conform
to the provisions of the internal monograph.
Even if not legally required, it is recommended that the supplier submits the monograph for
assessment by the FAMHP.
The general concept of the Belgian magistral phage medicine strategy is depicted in Figure 1.
A single characterized phage seed lot is selected from a phage bank. To prevent the unwanted drift of
properties resulting from repeated subcultures, the production of medicines obtained by microbial
culture is best based on a system of banked master and working seed lots. From this phage seed
lot, a phage API is produced according to a monograph. A Belgian Approved Laboratory performs
External Quality Assessments to evaluate the API’s properties and quality. Each batch of these phage
APIs will have a batch record, which describes the production process for that batch in detail. Phage
APIs can be produced by both private companies and public institutions. The phage API, accompanied
by its batch record protocol and the results of the External Quality Assessments, is then transferred to
the hospital pharmacy for possible incorporation in magistral formulas. Ideally, active phage APIs are
selected against the target bacteria. In comparison to an antibiogram (to test antibiotic sensitivity), as it
were, a “phagogram” is performed. Today, no formal guidelines exist with regard to the clinical use
(e.g., medical indications, formulations and posology) of magistral phage medicines. However, it is
Viruses 2018,10, 64 5 of 7
the intention to draft these guidelines as quickly as possible, at the Belgian level and possibly at the
European level.
Viruses 2018, 10, x FOR PEER REVIEW 5 of 6
Figure 1. General flowchart of the magistral phage medicine process.
3.3. Phage API Monograph
Next, experts of the Queen Astrid military hospital in Brussels, the FAMHP and the Belgian
Scientific Institute of Public Health elaborated a pragmatic supplier monograph for phage APIs with
a limited use (to hospital pharmacies) status. This document was conceived as a general (applicable
to most phages) and evolving document. On 10 January 2018, version 1.0 of the monograph
(Supplementary Document 1) received a formal positive advice by the FAMHP.
3.4. Pricing and Reimbursement
In terms of pricing, the total cost of a magistral preparation is a reflection of the costs for the
products in the preparation, eventually the costs of the prescribed excipients or recipients and an
honorarium for the pharmacist for the magistral preparation. Reimbursement of a magistral
preparation in Belgium is subject to several criteria: (1) the pharmacist receives a prescription from a
physician; (2) this pharmacist makes the magistral preparation and delivers it; (3) products in the
magistral preparation are listed on a predefined list of products eligible for reimbursement; and (4)
the conditions for reimbursement need to be respected. Bacteriophages are at the moment not listed
as products eligible for reimbursement. Therefore, depending on the ultimate price set for a phage
magistral preparation, this might (or not) influence the access of phage therapy to patients.
4. Conclusions
It seems to be a matter of time before phage therapy regains its status as an established
antibacterial tool. However, this will not only depend on the credibility of “phage researchers, but
also on the political context in which they are working. Phage therapy is not sustainable without
reimbursement of the researchers providing the therapeutic phages, and so far, phage research is
underfunded. Just as drug companies are allowed to profit for some time after developing a drug,
there must be some form of compensation for the investigators isolating, characterizing, and
optimizing the phages that will be included in future therapeutic phage banks, all before a pharmacist
gains access to them for combination. Phage researchers should not be expected to automatically be
“altruists”, and compensation must be given for their efforts at developing phage therapy as a
medicine.
Believing that Belgium could do some pioneering work in the phage therapy field, the Belgian
Minister of Public Health and the FAMHP opened the door to phage medicines that take into account
the unique characteristics of phages and the need for personalized “sur-mesure and sustainable
phage therapy approaches [7]. There is every reason to believe that the resulting Belgian “magistral
phage medicine” framework will be flexible enough to exploit and further explore the specific nature
Figure 1. General flowchart of the magistral phage medicine process.
3.3. Phage API Monograph
Next, experts of the Queen Astrid military hospital in Brussels, the FAMHP and the Belgian
Scientific Institute of Public Health elaborated a pragmatic supplier monograph for phage APIs
with a limited use (to hospital pharmacies) status. This document was conceived as a general
(applicable to most phages) and evolving document. On 10 January 2018, version 1.0 of the monograph
(Supplementary Document 1) received a formal positive advice by the FAMHP.
3.4. Pricing and Reimbursement
In terms of pricing, the total cost of a magistral preparation is a reflection of the costs for the
products in the preparation, eventually the costs of the prescribed excipients or recipients and
an honorarium for the pharmacist for the magistral preparation. Reimbursement of a magistral
preparation in Belgium is subject to several criteria: (1) the pharmacist receives a prescription from
a physician; (2) this pharmacist makes the magistral preparation and delivers it; (3) products in the
magistral preparation are listed on a predefined list of products eligible for reimbursement; and (4) the
conditions for reimbursement need to be respected. Bacteriophages are at the moment not listed
as products eligible for reimbursement. Therefore, depending on the ultimate price set for a phage
magistral preparation, this might (or not) influence the access of phage therapy to patients.
4. Conclusions
It seems to be a matter of time before phage therapy regains its status as an established antibacterial
tool. However, this will not only depend on the credibility of “phage researchers”, but also on the
political context in which they are working. Phage therapy is not sustainable without reimbursement
of the researchers providing the therapeutic phages, and so far, phage research is underfunded. Just as
drug companies are allowed to profit for some time after developing a drug, there must be some
form of compensation for the investigators isolating, characterizing, and optimizing the phages that
will be included in future therapeutic phage banks, all before a pharmacist gains access to them
Viruses 2018,10, 64 6 of 7
for combination. Phage researchers should not be expected to automatically be “altruists”, and
compensation must be given for their efforts at developing phage therapy as a medicine.
Believing that Belgium could do some pioneering work in the phage therapy field, the Belgian
Minister of Public Health and the FAMHP opened the door to phage medicines that take into account
the unique characteristics of phages and the need for personalized “sur-mesure” and sustainable
phage therapy approaches [
7
]. There is every reason to believe that the resulting Belgian “magistral
phage medicine” framework will be flexible enough to exploit and further explore the specific nature
of phages as co-evolving antibacterials whilst giving precedence to patients’ safety. Importantly,
this Belgian solution avoids the application of certain medicinal product requirements that restrain
flexible phage therapy approaches, such as compliance to Good Manufacturing Practice (GMP).
There are indications that other (EU) countries might also adopt this phage therapy framework in the
near future, in anticipation of a European solution. Recently, the biological master file concept was put
forward as a European solution to overcome the regulatory challenges of personalized medicines in
general and phage medicines more specifically [11].
Supplementary Materials:
The following are available online at www.mdpi.com/xxx/s1, Document 1. Phage
API monograph (version 1.0).
Author Contributions:
Jean-Paul Pirnay, Gilbert Verbeken, Pieter-Jan Ceyssens, Isabelle Huys, Daniel De Vos,
Charlotte Ameloot and Alan Fauconnier contributed to the conception and writing of this paper.
Conflicts of Interest: The authors declare no conflict of interest.
References
1.
Tackling Drug-Resistant Infections Globally: Final Report and Recommendations. The Review on
Antimicrobial Resistance. 2016 Release. Available online: https://amr-review.org/sites/default/files/
160525_Final%20paper_with%20cover.pdf (accessed on 4 February 2018).
2.
United Nations. Draft Political Declaration of the High-Level Meeting of the General Assembly on
Antimicrobial Resistance (16-16108 (E)). 2016 Release. Available online: http://www.un.org/pga/71/
wp-content/uploads/sites/40/2016/09/DGACM_GAEAD_ESCAB-AMR-Draft-Political-Declaration-
1616108E.pdf (accessed on 17 December 2017).
3.
Thiel, K. Old dogma, new tricks—21st Century phage therapy. Nat. Biotechnol.
2004
,22, 31–36. [CrossRef]
[PubMed]
4.
Hall, A.R.; De Vos, D.; Friman, V.P.; Pirnay, J.P.; Buckling, A. Effects of sequential and simultaneous
applications of bacteriophages on populations of Pseudomonas aeruginosa
in vitro
and in wax moth larvae.
Appl. Environ. Microbiol. 2012,78, 5646–5652. [CrossRef] [PubMed]
5.
Jennes, S.; Merabishvili, M.; Soentjens, P.; Pang, K.W.; Rose, T.; Keersebilck, E.; Soete, O.; François, P.M.;
Teodorescu, S.; Verween, G.; et al. Use of bacteriophages in the treatment of colistin-only-sensitive
Pseudomonas aeruginosa septicaemia in a patient with acute kidney injury—A case report. Crit. Care
2017
,21,
129. [CrossRef] [PubMed]
6.
Schooley, R.T.; Biswas, B.; Gill, J.J.; Hernandez-Morales, A.; Lancaster, J.; Lessor, L.; Barr, J.J.; Reed, S.L.;
Rohwer, F.; Benler, S.; et al. Development and use of personalized bacteriophage-based therapeutic cocktails
to treat a patient with a disseminated resistant Acinetobacter baumannii Infection. Antimicrob. Agents Chemother.
2017,61, e00954-17. [CrossRef] [PubMed]
7.
Pirnay, J.P.; De Vos, D.; Verbeken, G.; Merabishvili, M.; Chanishvili, N.; Vaneechoutte, M.; Zizi, M.; Laire, G.;
Lavigne, R.; Huys, I.; et al. The phage therapy paradigm: Prêt-à-porter or sur-mesure? Pharm. Res.
2011
,28,
934–937. [CrossRef] [PubMed]
8. Servick, K. Beleaguered phage therapy trial presses on. Science 2016,352, 1506. [CrossRef] [PubMed]
9.
Commission De La SantéPublique, De L’environnement Et Du Renouveau De La Société. Questions Jointes
De Mme Muriel Gerkens Et M. Philippe Blanchart ÀLa Ministre Des Affaires Sociales Et De La Santé
Publique Sur “La Phagothérapie” ÀLa Ministre Des Affaires Sociales Et De La SantéPublique” (N
11955
and N
12911). 2016 Release. Available online: https://www.dekamer.be/doc/CCRA/pdf/54/ac464.pdf
(accessed on 17 December 2017).
Viruses 2018,10, 64 7 of 7
10.
Fauconnier, A. Guidelines for Bacteriophage Product Certification. Methods Mol. Biol.
2018
,1693, 253–268.
[PubMed]
11.
Fauconnier, A. Regulating phage therapy: The biological master file concept could help to overcome
regulatory challenge of personalized medicines. EMBO Rep. 2017,18, 198–200. [CrossRef] [PubMed]
©
2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).
... This feature is essential in Africa, where AMR levels are high and last-resort antibiotics are scarce. Phages can also evolve alongside bacterial pathogens, potentially reducing the risk of resistance development and prolonging the efficacy of treatment (38,39). ...
Article
Full-text available
The increasing threat of antimicrobial resistance (AMR) in Africa, coupled with limited access to advanced antibiotics and high rates of bacterial infections poses serious public health challenge. Bacteriophages, viruses that target and destroy bacteria, present a promising alternative or complementary therapy to traditional antibiotics. Phage therapy leverages its unique ability to target specific bacterial strains without affecting the host beneficial microbiota. It is an effective tool against multi-drug-resistant (MDR) microbial pathogens, particularly in resource-limited settings. This narrative review explores the potentials of phage therapy in Africa, highlighting its advantages, such as specificity, minimal side effects, and cost-effectiveness, alongside its capability to tackle biofilm-associated and AMR infections. It discusses current research and collaborations, including case studies from Nigeria, Benin, and South Africa that demonstrate the efficacy of phage therapy against bacterial pathogens such as Escherichia coli, Acinetobacter baumannii and Klebsiella pneumoniae. Furthermore, it discusses the challenges to phage therapy implementation such as regulatory hurdles, public skepticism, and infrastructure limitations, while emphasizing the importance of developing local production and awareness campaigns. The review concludes by recommending the integration of phage therapy into Africa healthcare strategies to address AMR. Through strategic partnerships, education and regulatory frameworks, phage therapy could become a transformative solution, particularly for neglected diseases and infections common in low-resource settings. As Africa seeks innovative approaches to its growing AMR crisis, phage therapy stands out as a viable and adaptable option.
... Currently, there are no approved regulations for bacteriophage treatment. However, with increasing evidence of their therapeutic capacity, many governing bodies such as Australian Therapeutic Goods Administration, European Medicines Agency and the United States Food and Drug Administration are becoming more amenable to approving the compassionate and magistral use of bacteriophages in therapy [151,152]. Regulation following a framework set out for similar therapeutic goods [153,154] e.g. attenuated virus vaccines, may facilitate the use of bacteriophages in patients. ...
Article
Full-text available
Background There is no specific cure for periodontitis and treatment is symptomatic, primarily by physical removal of the subgingival plaque biofilm. Current non-surgical periodontal therapy becomes less effective as the periodontal pocket depth increases and as such new adjunctive treatments are required. The development of antibiotic resistance has driven a recent resurgence of interest in bacteriophage therapy. Methods Here we review the published literature with a focus on the subgingival phageome, key oral pathobionts and the dysbiotic nature of periodontitis leading to the emergence of synergistic, proteolytic and inflammophilic bacterial species in subgingival plaque. We discuss the opportunities available, the barriers and the steps needed to develop bacteriophage therapy as an adjunctive treatment for periodontitis. Results The oral phageome (or virome) is diverse, featuring abundant bacteriophage, that could target key subgingival bacteria. Yet to date few bacteriophages have been isolated and characterised from oral bacterial species, although many more have been predicted by genomic analyses. Bacteriophage therapy has yet to be tested against chronic diseases that are caused by dysbiosis of the endogenous microbial communities. Conclusion To be effective as an adjunctive treatment for periodontitis, bacteriophage therapy must cause the collapse of the dysbiotic bacterial community, thereby resolving inflammation and enabling the reestablishment of a health-associated mutualistic subgingival bacterial community. The isolation and characterisation of novel oral bacteriophage is an essential first step in this process.
Article
Full-text available
Prolonged and extensive use of antibiotics in clinical, veterinary, animal stock, agriculture, and food processing sectors are rendering them least effective. The pipeline for new antibiotics is almost dry, leading to the prevalence of multi-drug resistant (MDR) strains. Bacteriophages are native predators of bacteria and have been conventionally illustrated for the treatment of bacterial infections. However easy and large-scale availability of antibiotics in subsequent decades overshadowed the approach of using bacteriophages for treatment. Modern medicine is profoundly dependent on antibiotics and is now soon approaching an alarming exhaustion of anti-infective options. As a ray of hope, scientists and clinicians contemplate advances in phage application as potential and viable options. Various strategies devised to remodel the potential of bacteriophages prominently include genetically engineered phages wherein the antibacterial efficacy of the phage is enhanced to combat MDR bacteria. While in the case of bacteriophage encoded enzymes, broad spectrum antimicrobial proteins are produced in microbial factories harbouring the encoded genetic material. In this review, we discuss various bacteriophage models that emphasize the success of the phenomenon and recent biotechnological advancements that allow to repurpose bacteriophages as an alternate potential therapeutic approach to control drug resistance.
Article
Full-text available
To increase their throughput, reduce laboratory work and improve reproducibility, automation of bioprocesses is gaining in importance nowadays. This applies in particular to microbioreactors (MBRs), which can be easily integrated in highly parallelized and automated platforms and, therefore, be applied for screenings, cell‐based assays, and bioprocess development. One promising pharmaceutical application for MBRs is the performance of phage sensitivity tests called phagograms in phage therapy. However, there is no automated and parallelized platform available so far that fulfills the requirements of phagograms. Therefore, a novel highly parallelizable capillary‐wave microbioreactor (cwMBR) with a volume of 7 µL, which has already been successfully applied for phagograms, was extended by an in‐house built platform for automated fluid addition in the single‐digit nanoliter range. The cwMBR has a phage‐repellent hydrophilic glass surface. Furthermore, a custom‐made highly parallelizable device for biomass measurement in the lower microliter scale was developed and validated in the cwMBR. To prove the applicability of the platform for the generation of phagograms, a phagogram using Escherichia coli and automated phage addition was performed. The results indicate a clear lysis of the bacteria by the phages and thus confirm the applicability of performing automated phagograms in the highly parallelizable cwMBR platform.
Article
The biggest threat to global health, which claims 700,000 lives annually, is the appearance and quick spread of multidrug resistant diseases. The mortality rate is anticipated to rise in the following decade owing to the escalating issue of antibiotic resistance. This resistance is due to the misuse and overuse of antibiotics, allowing infectious bacteria to evolve resistance that makes treatments ineffective. Phage therapy, long buried by an antibiotic era, is enjoying a well-deserved renaissance as a consequence of the increase in antibiotic resistance leading to a variety of illnesses that are remediless. This rising concern has made the scientific body more cautious about developing an approach without considering its implications. While establishing an alternative therapy, numerous factors must be taken into account, including the relative benefits and drawbacks. Bacteriophages provide unique insight into the generation of innovative medicines that will lower the frequency of bacterial illnesses since they are host-specific. As the globe transitions away from the widespread use of antibiotics, this review describes the prospective utility, strategies, recent developments, and clinical studies of phage therapy while also highlighting contemporary treatments for MDR bacteria.
Article
Full-text available
Klebsiella pneumoniae is a notorious, Gram-negative pathogen and is a leading cause of healthcare settings and community-acquired infections. This is the commensal of human microbiota and can invade and cause infections in different body parts. The global emergence of antibiotic resistance in K. pneumoniae has become a major challenge in the whole medical community. Alternative paths to treat the infections caused by these MDR pathogens are needed as these bacteria become resistant to last-resort antibiotics like colistin. The lytic bacteriophages (phages) are the bacteria's natural predators and can rapidly eliminate the bacterial cells. Phages are abundant in nature and have recently been found to be effective tools in modern biotechnology. They can be used to control the bacterial infectious diseases. They can be manipulated easily and potentially used in therapeutics, biotechnology, and research. Several studies, both in vitro and in vivo, have demonstrated the possible applications of the lytic phages in treating K. pneumoniae superbug strains. Phage endolysins have drawn the scientific world's attention because of their involvement in phage adsorption and bacterial capsules digestion. These phage-encoded enzymes digest the polysaccharide components of bacterial cell walls by recognizing and binding them. Phage lysins, being strong biological agents, are capable of effectively and swiftly eliminating bacteria. This review summarizes the information on phages of K. pneumoniae and phage-based therapies to target their bacterial hosts.
Article
Full-text available
The growing problem of multi-drug resistance (MDR) is prevalent in Gram-negative infections, and the significant decline in antibiotic development poses a critical threat to global public health. Many emerging non-antibiotic therapies have been proposed, including phage therapy, anti-virulence agents, antimicrobial peptides, plasmapheresis, and immunotherapy options. To identify the therapies most likely to be the next immediate step in treatment for MDR Gram-negative infections, this review highlights emerging therapeutics that have either been successfully used for compassionate care or are currently undergoing clinical trials.
Article
Full-text available
Widespread antibiotic use in clinical medicine and the livestock industry has contributed to the global spread of multidrug-resistant (MDR) bacterial pathogens, including Acinetobacter baumannii . We report on a method used to produce a personalized bacteriophage-based therapeutic treatment for a 68-year old diabetic patient with necrotizing pancreatitis complicated by a MDR A. baumannii infection. Despite multiple antibiotic courses and efforts at percutaneous drainage of a pancreatic pseudocyst, the patient deteriorated over a four-month period. In the absence of effective antibiotics, two laboratories identified nine different bacteriophages with lytic activity for an A. baumannii isolate from the patient. Administration of these bacteriophages intravenously and percutaneously into the abscess cavities was associated with reversal of the patient's downward clinical trajectory, clearance of the A. baumannii infection, and a return to health. The outcome of this case suggests that the methods described here for the production of bacteriophage therapeutics could be applied to similar cases and that more concerted efforts to investigate the use of therapeutic bacteriophages for MDR bacterial infections are warranted.
Article
Full-text available
Interest in using bacteriophages to treat bacterial infections (phage therapy) is growing, but there have been few experiments comparing the effects of different treatment strategies on both bacterial densities and resistance evolution. While it is established that multiphage therapy is typically more effective than the application of a single phage type, it is not clear if it is best to apply phages simultaneously or sequentially. We tried single- and multiphage therapy against Pseudomonas aeruginosa PAO1 in vitro, using different combinations of phages either simultaneously or sequentially. Across different phage combinations, simultaneous application was consistently equal or superior to sequential application in terms of reducing bacterial population density, and there was no difference (on average) in terms of minimizing resistance. Phage-resistant bacteria emerged in all experimental treatments and incurred significant fitness costs, expressed as reduced growth rate in the absence of phages. Finally, phage therapy increased the life span of wax moth larvae infected with P. aeruginosa, and a phage cocktail was the most effective short-term treatment. When the ratio of phages to bacteria was very high, phage cocktails cured otherwise lethal infections. These results suggest that while adding all available phages simultaneously tends to be the most successful short-term strategy, there are sequential strategies that are equally effective and potentially better over longer time scales.
Chapter
Following decades in the wilderness, bacteriophage therapy is now appearing as a credible antimicrobial strategy. However, this reemerging therapy does not rekindle without raising sensitive regulatory concerns. Indeed, whereas the European regulatory framework has been basically implemented to tackle ready-to-use pharmaceuticals produced on a large scale, bacteriophage therapy relies on a dynamic approach requiring a regulation on personalized medicine, nonexistent at present. Because of this, no guideline are currently available for addressing the scientific and regulatory issues specifically related to phage therapy medicinal products (PTMP).
Article
In a note published in The Lancet in December 1915, Frederic Twort described the discovery of an infectious, filterable agent that causes the glassy transformation and eventual killing of bacteria, now identified as Staphylococcus hyicus [1]. Two years later, Felix d'Herelle independently described an invisible microbe antagonistic to dysentery bacilli in a note to the Comptes Rendus de l'Academie des Sciences [2]. These two reports are the first published descriptions of bacteriophages, a term coined by d'Herelle, who foresaw the therapeutic potential of these newly identified bacteriolytic agents. > The key issue is whether phage therapy medicinal products (PTMPs) require a marketing authorization or not. Yet, phage therapy, despite varying degrees of success during the 20th century, never made it into widespread clinical use owing to the discovery of antibiotics [3]. However, the increasing antibiotic resistance among major pathogens such as Staphylococcus or Mycobacterium has become a critical public health problem—in the European Union (EU) alone, around 25,000 patients die each year from an infection with drug‐resistant bacteria (http://ecdc.europa.eu/en/publications/publications/0909\_ter\_the\_bacterial\_challenge\_time\_to\_react.pdf). Although difficult to estimate, the annual global death toll attributable to antimicrobial resistance might range from the hundreds of thousands to as much as millions in the coming decades (https://amr-review.org/sites/default/files/AMR%20Review%20Paper%20-%20Tackling%20a%20crisis%20for%20the%20health%20and%20wealth%20of%20nations\_1.pdf). In light of this growing health crisis, phage therapy has attracted renewed interest as an alternative to antibiotics [3]. ### Regulatory challenges for phage therapy However, this reemergence raises regulatory concerns. There has been intense discussion on how to classify phage‐based therapeutics within the EU's regulatory framework [4], [5], [6] with some consensus among Members States' regulatory authorities and the European Medicines Agency that these would be regulated as biological medicinal products [7]. Nonetheless, positions still vary because the current medicinal product regulation is not well suited for this unorthodox therapeutic …
Article
In the face of rising antibiotic resistance, many researchers hope that bacteria-killing viruses known as phages—long available to patients in Eastern Europe—will offer patients in the West with dangerous infections an alternative treatment. A European clinical trial envisioned as the first large-scale test of phages under modern regulatory standards was expected to have results this summer. But after a series of delays, the trial, known as PhagoBurn, has been forced to shrink in size and scope. Now, it's racing to recruit patients and produce results by early next year. The project may blaze a path for future products to seek market approval, but it also illustrates some of the many obstacles they'll face in demonstrating that phages are safe and effective.
Article
As antibiotic resistant bacteria threaten a public health crisis, biotechnology is turning to bacteriophages, nature's tiniest viruses. But can phage therapy overcome its historical baggage?