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Endermologie New Aproach in the Medicine Treatment


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Using the effect of mechanical forces affecting cellular response in the treatment of post-traumatic, postoperative, post-imlantation conditions through the application of Endermologie®- mechanotransduction represents a revolutionary solution in tissue-rehabilitation and positive target tissue influencing, with faster regeneration (1). Endermologie® is a noninvasive, painless, natural method of treatments of all connective tissue transformations, muscle and circulation pathologies. The aim of our study is investigation and explanation the mechanism of action by observing the physiological effects of Endermologie® based on human studies. The paper is focused on monitoring of possitive effect tissue regeneration using endermologie as a tools mechanostimulation improvements of systems integridy and health improvement.
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TECHNOLOGICAL ENGINEERING, Volume XIV, number 1/2017, ISSN 1336 - 5967
Article history:
Received 20.8.2017
Accepted 17.9.2017
Available online 1.12.2017
Viktória Mezencevová1,4 - Jozef Torok2 - Tatiana Czánová3 - Ján Zajac4
1 M-Science Group, s.r.o., Košice, Slovakia
2 Faculty of Manufacturing Technologies Technical University of Košice with a seat
in Presov, Slovakia
3 Faculty of Mechanical Engineering, University of Zilina, Slovakia
4 Faculty of Mechanical Engineering, Technical University of Košice,Slovakia
Using the effect of mechanical forces affecting cellular
response in the treatment of post-traumatic, postope-
rative, post-imlantation conditions through the application
of Endermologi- mechanotransduction represents a
revolutionary solution in tissue-rehabilitation and positive
target tissue influencing, with faster regeneration (1).
Endermologie® is a noninvasive, painless, natural me-
thod of treatments of all connective tissue transforma-
tions, muscle and circulation pathologies. The aim of our
study is investigation and explanation the mechanism of
action by observing the physiological effects of
Endermologie® based on human studies.
The paper is focused on monitoring of possitive effect
tissue regeneration using endermologie as a tools
mechanostimulation improvements of systems integridy
and health improvement..
Endermologie®, connective tissue, mechanotrans-
duction, scar,
More than 30 years ago, a new non-invasive and natural
way to treat traumatic changes in connective tissue, skin
and the like has been discovered, with a proven positive
effect on the regeneration and rehabilitation of systemic
tissues through mechanical cell stimulation.
End 70.r. In the 20th century, engineer Loius Paul Guitay
suffered extensive skin injuries, muscles as a result of
burns and scarring, resulting in increased adherence,
tissue adhesion, loss of elasticity, momentum,
attenuation of vascular and lymphatic circulation,
increased stagnation of interstitial fluid, edema, fibrotic
changes, decreased oxygenation of trophic tissues, and
progression of degradation changes.
In order to streamline the therapeutic process, in order to
maximize shortening of the rehabilitation and
regeneration period, to standardize treatment at any
time within circadian biorhythms (elimination of negative
human factors such as fatigue, exhaustion, disparity in
the administration of procedures by individuals within the
day) Louiom P. Gutayiom new system in terpaii called "
Endermológia®. in conjunction with mechanical forces
and physiotherapy techniques to achieve a better effect,
in less time and under uniform conditions than manual
physiotherapy-rehabilitation techniques.
ENDERMOLOGY and LPG techniques
Endermológia® LPG® is a non-invasive, non-invasive,
100% natural, patented method of mechanical
stimulation of cells, aimed at systemic tissue
manipulation through application to the skin.
Endermology represents interaction between the dosed
vacuum and roller massage, controlled aspiratory force,
frequency, controlled and controlled velocity and
direction of roller movement.
Figure 1. Single motorized rollers ©LPG® Systems, 2004
LPG® cell mechano-stimulation is carried out by specific
software-controlled monitored therapeutic heads, tissue-
specific so- keymodules, equipped with unique rollers
and flaps, allowing personalized skin care treatments
using the exclusive patented Roll and Lift techniques.
The working head of the device with two completely
independently driven and moving rollers (Fig. 1) creates a
vacuum wave which moves forward, backward, sideways
or diagonally by a self-propelled head, pulse aspirations.
The rollers represent autonomous motorized units
operating at their own speed and direction of movement.
(Fig. 2)
volume XIV, number 1/2017
ISSN 1336 - 5967
DOI: 10.1515/teen-2017-0007
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TECHNOLOGICAL ENGINEERING, Volume XIV, number 1/2017, ISSN 1336 - 5967
Figure 2. Traction of the skin [25]
Figure 3. Traction of the skin and connective fibres [25]
Variation of combinations allows an infinite number
of ways of rolling the skin depending on the type and
condition, respectively. pathology of the treated tissue
(Fig. 4). Depending on the method and speed of
movement of the individual rollers relative to each other,
and depending on the aspiration we distinguish the so-
called Roll in (e.g., fat elimination, spasm, β-receptor
stimulation on adipocytes, myorelaxation), Roll up (e.g.,
fastening, anti-dermatosis, myorelaxis), Roll out (eg
elasticity, stiffness and tissue formation).
Figure 4. Specific modules for individual tissues
© LPG Systems 2004
Post-transplant post-traumatic postoperative post-
transplantation therapies use specific personalized
protocols using LPG® Mechanostimulation with many
3x faster regeneration
Non-invasive, painless, natural therapy
Stimulation of vascular and lymphatic flow
Increased tissue oxygenation, trophism
Elimination of toxins, catabolites, lactic acid etc.
Removing damages of the connective tissues, of the
skin and muscles
Elimination of edema, pain, fatigue, spasm, muscle
contractions etc.
Proven effectiveness of Endermotherapy:
Treatment of scars, burns
Venous insufficiency
Indurations, edema, fibrosis
Sclerosis multiplex
Increased muscular elasticity
Detoxification of the muscles and connective tissues
Increase in load tolerance
Rehabilitation, regeneration, relaxation, vitality
The discovery of mechanical forces as possible cell
growth regulators, respectively. degradation, and
regeneration of the skeletal muscle and heart tissue are
a major challenge in the field of cellular and tissue
engineering. Mechanisms of mechanical signaling and
cellular mechanotransduction will be explained to
develop new therapies [2].
Mechanotransduction leads to processes by which
cells perceive mechanical stimuli and respond to them by
converting them into biochemical signals resulting in a
specific cellular response. Mechanical stimuli are known
to be as important for cells as biochemical [3].
The mechanism of action of the mechanical forces
affecting the cellular response that is important in the
disease development process is not yet fully understood
[4]. Recent studies [5] show that the mechanical stress of
epithelial cells activates the transcription factors YAP1
and β-catenin, depending on the dose of cadherin, and
causes cell cycle triggering with S phase progression.
Experimental findings [6] demonstrating the effect of
substrate elasticity on direct directing of differentiation of
human mesenchymal cells (hMSCs) into different lines
(eg fibroblast osteoblastic, myoblastic) are of particular
significance. There is evidence [7,8,9] that key mechanic
regulators of cell adhesion, contractility, gene regulation,
cell matrix interactions play actinomyosin fibers in
hMSCs [7,18, 20, 21] whose structure and organization
show significant differences in the early stages of
mechanically induced differentiation (up to 24 hr)
depending on substrate elasticity.
Recent studies [10] demonstrated, in addition to the
effect of substrate elasticity on myoblast differentiation,
that mechanical stimuli and possible changes in the
Roll in
Roll out
Roll up
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TECHNOLOGICAL ENGINEERING, Volume XIV, number 1/2017, ISSN 1336 - 5967
cytoskeletal structure play an important role in the
differentiation of more than one cell type.
Experimental evidence [5, 19, 22, 23] also confirms
that mechanical stimulation of cells transduces mecha-
nical signals into transcriptional responses, and that
transcriptional activity is important for cell cycle rebirth
and progression.
The positive effect of Endermoterapie and LPG®
techniques in cell and systemic stimulation processes
was experimentally demonstrated in more
as 140 scientific publications COSIRE (International
Scientific Research Committee).
Significant progress in the treatment of classic
treatment-resistant lymphoedema (MLD, manual lymph
drainage) and statistically significant improvement in all
parameters monitored after endermotherapy was
reported in independent studies [11,12]. Microlium
techniques have demonstrated the positive effect of
LPG® on the superficial lymphatic network, measuring
body fluid volumes demonstrated a statistically
significant reduction in the size of edema, improvement
in vascular and lymphatic circulation (Doppler).
In all types of endermotherapy, standardization,
personification, shortening of duration and duration of
treatment, permanent monitoring, prolonged positive
effect were also demonstrated.
Priority applications in the treatment of burns (Fig.
5), scarring (Figures 6,7), muscles were applied in order
to maximize the efficiency and shortening of treatment
time, mobilization of individual traumatized tissues
without pain, elimination of fibrotic barriers, adhesion,
edema, elasticity, densities, oxygenation, and trophism
of tissues have given rise to secondary applications in
the field of endermoestetics due to significant
discoveries of increased cell proliferation of fibroblast
cells and stimulation of lipolytic activity of adipocytes
[14,15,16, 17].
Histologically and stereophotometrically, the
positive effect of tangential endermo-stimulation of the
facial skin has been demonstrated through the
mechanical force induced in fibroblasts. In more than
80% of treated patients there was a significant clinical
improvement in skin quality (tonus, turgor, edema and
fat elimination) and structural changes in the papillary
dermis (increased production of pro-collagen and pro-
elastin fibers, compaction of collagen and elastin)
increased elasticity, skin compactness, elimination of
fibrotic changes, locally stored fat, wrinkle elimination
[13, 25, 26, 27].
For over 30 years, LPG® has been the leader in
connective tissue therapy. Lafontan [14, 15, 16] by
microdialysis and DNA analysis of chips showed an
increased lipolytic response to LPG® mechano-
stimulation, through increased activity by + 70% β-
adipocyte receptors.
At the same time, the absence of inflammatory
factors has been demonstrated, demonstrating that this
highly effective therapy mobilizes fat metabolism
without traumatic injury.
Humbert demonstrated that mechanical
transduction of fibroblast cells stimulates their cellular
activity: increases migration capacity (+ 14%), induces
extracellular matrix remodeling (ECM), stimulates
differentiation of fibroblasts into myofibroblasts [17, 28,
Experimentally, LPG® mechanotransduction of
fibroblast cells has been shown to increase significantl
cellular proliferation, DNA synthesis and
proteosynthetic activity + 240% collagen, + 130%
elastin [24,17].. The reversal discovery was evidence of
increased production of endogenous hyaluronic acid by
+80, 2% (see Fig. 3), [17].
Figure 5. Increased production of endogenous hyaluronic
acid, from tissue biopsy [17]
Figure 6. Enderotherapii treatment of the scars © LPG
Systems 2004
Figure 7. Inflamation scar treatment © LPG Systems
Figure 8. Effect of endermotherapy in post titanium
implant application before, and after 10 treatments [1]
© M-Science Group
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TECHNOLOGICAL ENGINEERING, Volume XIV, number 1/2017, ISSN 1336 - 5967
Integration of engineering principles through the
application of innovative technology utilizing
Endermological® LPG® techniques and the
mechanotransduction of tissue in the process of
targeted personified post-transplantation therapy
provide effective and new therapeutic tools in tissue
and systems regeneration and rehabilitation.
This article was funded by the University of Žilina
project APVV 15-0405 “Complex use of X-ray
diffractometry for identification and quantification of
functional properties of dynamically loaded structural
elements from important technical materials”
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Full-text available
Since most of the implant surface is in direct contact with bone tissue, shape and integrity of said surface has great influence on successful osseointegration. Among other characteristics that predetermine titanium of different grades of pureness as ideal biomaterial, titanium shows high mechanical strength making precise miniature machining increasingly difficult. Current titanium-based implants are often anodized due to colour coding. This anodized layer has important functional properties for right usage and also bio-compatibility of dental implants. Physical method of anodizing and usage of anodizing mediums has a significant influence on the surface quality and itself functionality. However, basic requirement of the dental implant with satisfactory properties is quality of machined surface before anodizing. Roughness, for example, is factor affecting of time length of anodizing operation and so whole productivity. The paper is focused on monitoring of surface and area characteristics, such as roughness or surface integrity after different cutting conditions of miniature machining of dental implants and their impact on suitability for creation of satisfactory anodized layer with the correct biocompatible functional properties.
Full-text available
Surface integrity has been an important research topic in the last decades. Machining generates residual stresses in the surface and subsurface layers of the structural elements. The residual stress has a large influence on the functional properties of the components. X-ray diffractometry is a non-destructive method applicable for the measurement of residual stresses in surface and subsurface layers of components. The article deals with the method of triaxial measurement of residual stress after machining the surface of sample by high feed milling technology. Significance of triaxial measuring is the capability of measuring in different angles so it is possible to acquire stress tensor containing normal and shear stress components acting in the spot of measuring, using a Cartesian coordinate system. For comparison, a simple measurement of residual stresses in the point indicates the stress direction only in normal direction of the measured surface.
Full-text available
One of the most best-known characteristic and important requirement of dental implant is made of biomaterials ability to create correct interaction between implant and human body. The most implemented material in manufacturing of dental implants is titanium of different grades of pureness. Since most of the implant surface is in direct contact with bone tissue, shape and integrity of said surface has great influence on the successful osseointegration. Among other characteristics of titanium that predetermine ideal biomaterial, it shows a high mechanical strength making precise machining miniature Increasingly difficult. The article is focused on evaluation of the resulting quality, integrity and characteristics of dental implants surface after machining.
Full-text available
Mechanical strain regulates the development, organization, and function of multicellular tissues, but mechanisms linking mechanical strain and cell-cell junction proteins to cellular responses are poorly understood. Here, we showed that mechanical strain applied to quiescent epithelial cells induced rapid cell cycle reentry, mediated by independent nuclear accumulation and transcriptional activity of first Yap1 and then β-catenin. Inhibition of Yap1- and β-catenin-mediated transcription blocked cell cycle reentry and progression through G1 into S phase, respectively. Maintenance of quiescence, Yap1 nuclear exclusion, and β-catenin transcriptional responses to mechanical strain required E-cadherin extracellular engagement. Thus, activation of Yap1 and β-catenin may represent a master regulator of mechanical strain-induced cell proliferation, and cadherins provide signaling centers required for cellular responses to externally applied force. Copyright © 2015, American Association for the Advancement of Science.
Full-text available
Physical features of microenvironments such as matrix elasticity E can clearly influence cell morphology and cell phenotype, but many differences between model matrices raise questions as to whether a standard biological scale for E exists, especially in 3D as well as in 2D. An E-series of two distinct types of hydrogels are ligand-functionalized here with non-fibrous collagen and used to elucidate wide-ranging cell and cytoskeletal responses to E in both 2D and 3D matrix geometries. Cross-linked hyaluronic acid (HA) based matrices as well as standard polyacrylamide (PA) hydrogels show that, within hours of initial plating, the adhesion, asymmetric shape, and cytoskeletal order within mesenchymal stem cells generally depend on E nonmonotonically over a broad range of physiologically relevant E. In particular, with overlays of a second matrix the stiffer of the upper or lower matrix dominates key cell responses to 3D: the cell invariably takes an elongated shape that couples to E in driving cytoplasmic stress fiber assembly. In contrast, embedding cells in homogeneous HA matrices constrains cells to spherically symmetric shapes in which E drives the assembly of a predominantly cortical cytoskeleton. Non-muscle myosin II generates the forces required for key cell responses and is a target of a phospho-Tyrosine signaling pathway that likely regulates contractile assemblies and also depends nonmonotonically on E. The results can be understood in part from a theory for stress fiber polarization that couples to matrix elasticity as well as cell shape and accurately predicts cytoskeletal order in 2D and 3D, regardless of polymer system.
Objective: For several years now, tissue mechanostimulation techniques have been used in rehabilitation medicine, aesthetic medicine and reconstructive and aesthetic plastic surgery(1). The devices consist of a system of rollers and mechanized fins that act in conjunction with an adjustable suction unit. The many studies that have followed over the years, while showing the validity of the method in the treatment of scars and burns, have underscored the limits consisting mainly in the contraindications to treat patients at an early stage and the slowness of results, which all too often depended on the operator's skill(2-5). The aim of our study was to evaluate whether those limits have been overcome by the latest generation of equipment, which should allow for immediate intervention and standardized and hence repeatable treatments, using new handpieces and computerized protocols. Methods :A total of 26 patients, aged between 16 and 82 years, were assessed: 12 patients suffered from post-traumatic scars, 6 patients from burns, and 8 patients had cosmetically unsatisfactory surgical scars in exposed areas of the body. The mechanostimulation sessions, performed biweekly, ranged from a minimum of 8 to a maximum of 20. Results: All patients completed treatment, while one patient did not come for check-ups. The method has proven to have absolutely no side effects except a slight tenderness shown during treatments by two patients with burns. All patients showed an improvement of at least one of the subjective symptoms (pain, paresthesia, feeling of paperlike skin). The majority of patients also noted improving scar appearance: in 9 subjects there was no need for lipofilling or previously scheduled revision surgery. Conclusions: The new handpieces, due largely to the fins and pulsed stimulation, allow for early treatment, depend less on the operator, and enable faster and more predictable results than those that are obtained from previous generation equipment.
We have quantitatively determined how the morphology and adhesion strength of myoblast cells can be regulated by photocurable gelatin gels, whose mechanical properties can be fine-tuned by a factor of 103 (0.1 kPa  E  140 kPa). The use of such gels allows for the investigation of mechano-sensing of cells not only near the natural mechanical microenvironments (E ~ 10 kPa) but also far below and beyond of the natural condition. Optical microscopy and statistical image analysis revealed that myoblast cells sensitively adopt their morphology in response to the substrate elasticity at E = 1 - 20 kPa, which can be characterized by the significant changes in the contact area and order parameters of actin cytoskeletons. In contrast, the cells in contact with the gels with lower elastic moduli remained almost round, and the increase in the elasticity beyond E ~ 20 kPa caused no distinct change in morphology. In addition to the morphological analysis, the adhesion strength was quantitatively evaluated by measuring the critical detachment pressure with an aid of intensive pressure waves induced by picosecond laser pulses. This non-invasive technique utilizing extremely short pressure waves (pulse time width ~ 100 ns) enables one to determine the critical pressure for cell detachment with reliable statistics while minimizing the artifacts arising from the inelastic deformation of cells. The adhesion strength also exhibited a transition from weak adhesion to strong adhesion within the same elasticity range (E = 1 - 20 kPa). A clear correlation between the cell morphology and adhesion strength suggests the coupling of the strain of the substrate and the mechanosensors near focal adhesion sites.
Abstract The current knowledge of the structure, expression and functions of fibronectin is reviewed.Fibronectin is a high molecular weight glycoprotein present in the blood, connective tissue and at cell surfaces. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans. It also interacts with cell surfaces as shown by the fact that fibronectincollagen complexes, or fibronectin alone when insolubilized on a surface such as plastic, enhances the attachment of various types of cells to such surfaces.It seems that fibronectin, through its binding to collagen and to the cell surface, forms a bridge between the cell and its surrounding matrix. Circulating fibronectin may participate in the formation of extracellular matrix in tissues. It may also function as a nonspecific opsonin designed to facilitate the uptake of tissue debris by phagocytic cells.Studies aimed at elucidating the significance of fibronectin in physiological phenomena and in disease have only just begun. The abundance of fibronectin in basement membrane structures and the developmental changes observed in its expression lead one to believe that the attachment (or lack of it) of cells to fibronectin plays a significant role in morphogenetic events or in normal development. Malignantly transformed cells tend to lack cell-associated fibronectin. The significance of the lack of surface fibronectin in transformed cells is a matter of some controversy, but if fibronectin is indeed the main mechanism that anchors cells to the extracellular matrix, disturbances of this mechanism could play an important role in malignancy and many other diseases.