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Endermologie New Aproach in the Medicine Treatment

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Using the effect of mechanical forces affecting cellular response in the treatment of post-traumatic, postoperative, post-imlantation conditions through the application of Endermologie®- mechanotransduction represents a revolutionary solution in tissue-rehabilitation and positive target tissue influencing, with faster regeneration (1). Endermologie® is a noninvasive, painless, natural method of treatments of all connective tissue transformations, muscle and circulation pathologies. The aim of our study is investigation and explanation the mechanism of action by observing the physiological effects of Endermologie® based on human studies. The paper is focused on monitoring of possitive effect tissue regeneration using endermologie as a tools mechanostimulation improvements of systems integridy and health improvement.
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TECHNOLOGICAL ENGINEERING, Volume XIV, number 1/2017, ISSN 1336 - 5967
27
ENDERMOLOGIE NEW APROACH IN THE MEDICINE
TREATMENT
Article history:
Received 20.8.2017
Accepted 17.9.2017
Available online 1.12.2017
Viktória Mezencevová1,4 - Jozef Torok2 - Tatiana Czánová3 - Ján Zajac4
1 M-Science Group, s.r.o., Košice, Slovakia
2 Faculty of Manufacturing Technologies Technical University of Košice with a seat
in Presov, Slovakia
3 Faculty of Mechanical Engineering, University of Zilina, Slovakia
4 Faculty of Mechanical Engineering, Technical University of Košice,Slovakia
Abstract
Using the effect of mechanical forces affecting cellular
response in the treatment of post-traumatic, postope-
rative, post-imlantation conditions through the application
of Endermologi- mechanotransduction represents a
revolutionary solution in tissue-rehabilitation and positive
target tissue influencing, with faster regeneration (1).
Endermologie® is a noninvasive, painless, natural me-
thod of treatments of all connective tissue transforma-
tions, muscle and circulation pathologies. The aim of our
study is investigation and explanation the mechanism of
action by observing the physiological effects of
Endermologie® based on human studies.
The paper is focused on monitoring of possitive effect
tissue regeneration using endermologie as a tools
mechanostimulation improvements of systems integridy
and health improvement..
Keywords
Endermologie®, connective tissue, mechanotrans-
duction, scar,
1 INTRODUCTION
More than 30 years ago, a new non-invasive and natural
way to treat traumatic changes in connective tissue, skin
and the like has been discovered, with a proven positive
effect on the regeneration and rehabilitation of systemic
tissues through mechanical cell stimulation.
End 70.r. In the 20th century, engineer Loius Paul Guitay
suffered extensive skin injuries, muscles as a result of
burns and scarring, resulting in increased adherence,
tissue adhesion, loss of elasticity, momentum,
attenuation of vascular and lymphatic circulation,
increased stagnation of interstitial fluid, edema, fibrotic
changes, decreased oxygenation of trophic tissues, and
progression of degradation changes.
In order to streamline the therapeutic process, in order to
maximize shortening of the rehabilitation and
regeneration period, to standardize treatment at any
time within circadian biorhythms (elimination of negative
human factors such as fatigue, exhaustion, disparity in
the administration of procedures by individuals within the
day) Louiom P. Gutayiom new system in terpaii called "
Endermológia®. in conjunction with mechanical forces
and physiotherapy techniques to achieve a better effect,
in less time and under uniform conditions than manual
physiotherapy-rehabilitation techniques.
ENDERMOLOGY and LPG techniques
Endermológia® LPG® is a non-invasive, non-invasive,
100% natural, patented method of mechanical
stimulation of cells, aimed at systemic tissue
manipulation through application to the skin.
Endermology represents interaction between the dosed
vacuum and roller massage, controlled aspiratory force,
frequency, controlled and controlled velocity and
direction of roller movement.
Figure 1. Single motorized rollers ©LPG® Systems, 2004
LPG® cell mechano-stimulation is carried out by specific
software-controlled monitored therapeutic heads, tissue-
specific so- keymodules, equipped with unique rollers
and flaps, allowing personalized skin care treatments
using the exclusive patented Roll and Lift techniques.
The working head of the device with two completely
independently driven and moving rollers (Fig. 1) creates a
vacuum wave which moves forward, backward, sideways
or diagonally by a self-propelled head, pulse aspirations.
The rollers represent autonomous motorized units
operating at their own speed and direction of movement.
(Fig. 2)
TECHNOLOGICAL ENGINEERING
volume XIV, number 1/2017
ISSN 1336 - 5967
DOI: 10.1515/teen-2017-0007
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Figure 2. Traction of the skin [25]
Figure 3. Traction of the skin and connective fibres [25]
Variation of combinations allows an infinite number
of ways of rolling the skin depending on the type and
condition, respectively. pathology of the treated tissue
(Fig. 4). Depending on the method and speed of
movement of the individual rollers relative to each other,
and depending on the aspiration we distinguish the so-
called Roll in (e.g., fat elimination, spasm, β-receptor
stimulation on adipocytes, myorelaxation), Roll up (e.g.,
fastening, anti-dermatosis, myorelaxis), Roll out (eg
elasticity, stiffness and tissue formation).
Figure 4. Specific modules for individual tissues
© LPG Systems 2004
Post-transplant post-traumatic postoperative post-
transplantation therapies use specific personalized
protocols using LPG® Mechanostimulation with many
benefits:
3x faster regeneration
Non-invasive, painless, natural therapy
Stimulation of vascular and lymphatic flow
Increased tissue oxygenation, trophism
Elimination of toxins, catabolites, lactic acid etc.
Removing damages of the connective tissues, of the
skin and muscles
Elimination of edema, pain, fatigue, spasm, muscle
contractions etc.
Proven effectiveness of Endermotherapy:
Treatment of scars, burns
lymphedemas
Venous insufficiency
Fibromyalgia
Indurations, edema, fibrosis
Scleroderma,
Sclerosis multiplex
Obesity
Increased muscular elasticity
Detoxification of the muscles and connective tissues
Increase in load tolerance
Rehabilitation, regeneration, relaxation, vitality
The discovery of mechanical forces as possible cell
growth regulators, respectively. degradation, and
regeneration of the skeletal muscle and heart tissue are
a major challenge in the field of cellular and tissue
engineering. Mechanisms of mechanical signaling and
cellular mechanotransduction will be explained to
develop new therapies [2].
2 MECHANOTRANSDUCTION
Mechanotransduction leads to processes by which
cells perceive mechanical stimuli and respond to them by
converting them into biochemical signals resulting in a
specific cellular response. Mechanical stimuli are known
to be as important for cells as biochemical [3].
The mechanism of action of the mechanical forces
affecting the cellular response that is important in the
disease development process is not yet fully understood
[4]. Recent studies [5] show that the mechanical stress of
epithelial cells activates the transcription factors YAP1
and β-catenin, depending on the dose of cadherin, and
causes cell cycle triggering with S phase progression.
Experimental findings [6] demonstrating the effect of
substrate elasticity on direct directing of differentiation of
human mesenchymal cells (hMSCs) into different lines
(eg fibroblast osteoblastic, myoblastic) are of particular
significance. There is evidence [7,8,9] that key mechanic
regulators of cell adhesion, contractility, gene regulation,
cell matrix interactions play actinomyosin fibers in
hMSCs [7,18, 20, 21] whose structure and organization
show significant differences in the early stages of
mechanically induced differentiation (up to 24 hr)
depending on substrate elasticity.
Recent studies [10] demonstrated, in addition to the
effect of substrate elasticity on myoblast differentiation,
that mechanical stimuli and possible changes in the
Roll in
Roll out
Roll up
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cytoskeletal structure play an important role in the
differentiation of more than one cell type.
Experimental evidence [5, 19, 22, 23] also confirms
that mechanical stimulation of cells transduces mecha-
nical signals into transcriptional responses, and that
transcriptional activity is important for cell cycle rebirth
and progression.
3 SCIENTIFIC EFFECTS
The positive effect of Endermoterapie and LPG®
techniques in cell and systemic stimulation processes
was experimentally demonstrated in more
as 140 scientific publications COSIRE (International
Scientific Research Committee).
Significant progress in the treatment of classic
treatment-resistant lymphoedema (MLD, manual lymph
drainage) and statistically significant improvement in all
parameters monitored after endermotherapy was
reported in independent studies [11,12]. Microlium
techniques have demonstrated the positive effect of
LPG® on the superficial lymphatic network, measuring
body fluid volumes demonstrated a statistically
significant reduction in the size of edema, improvement
in vascular and lymphatic circulation (Doppler).
In all types of endermotherapy, standardization,
personification, shortening of duration and duration of
treatment, permanent monitoring, prolonged positive
effect were also demonstrated.
Priority applications in the treatment of burns (Fig.
5), scarring (Figures 6,7), muscles were applied in order
to maximize the efficiency and shortening of treatment
time, mobilization of individual traumatized tissues
without pain, elimination of fibrotic barriers, adhesion,
edema, elasticity, densities, oxygenation, and trophism
of tissues have given rise to secondary applications in
the field of endermoestetics due to significant
discoveries of increased cell proliferation of fibroblast
cells and stimulation of lipolytic activity of adipocytes
[14,15,16, 17].
Histologically and stereophotometrically, the
positive effect of tangential endermo-stimulation of the
facial skin has been demonstrated through the
mechanical force induced in fibroblasts. In more than
80% of treated patients there was a significant clinical
improvement in skin quality (tonus, turgor, edema and
fat elimination) and structural changes in the papillary
dermis (increased production of pro-collagen and pro-
elastin fibers, compaction of collagen and elastin)
increased elasticity, skin compactness, elimination of
fibrotic changes, locally stored fat, wrinkle elimination
[13, 25, 26, 27].
For over 30 years, LPG® has been the leader in
connective tissue therapy. Lafontan [14, 15, 16] by
microdialysis and DNA analysis of chips showed an
increased lipolytic response to LPG® mechano-
stimulation, through increased activity by + 70% β-
adipocyte receptors.
At the same time, the absence of inflammatory
factors has been demonstrated, demonstrating that this
highly effective therapy mobilizes fat metabolism
without traumatic injury.
Humbert demonstrated that mechanical
transduction of fibroblast cells stimulates their cellular
activity: increases migration capacity (+ 14%), induces
extracellular matrix remodeling (ECM), stimulates
differentiation of fibroblasts into myofibroblasts [17, 28,
29].
Experimentally, LPG® mechanotransduction of
fibroblast cells has been shown to increase significantl
cellular proliferation, DNA synthesis and
proteosynthetic activity + 240% collagen, + 130%
elastin [24,17].. The reversal discovery was evidence of
increased production of endogenous hyaluronic acid by
+80, 2% (see Fig. 3), [17].
Figure 5. Increased production of endogenous hyaluronic
acid, from tissue biopsy [17]
Figure 6. Enderotherapii treatment of the scars © LPG
Systems 2004
Figure 7. Inflamation scar treatment © LPG Systems
2004
Figure 8. Effect of endermotherapy in post titanium
implant application before, and after 10 treatments [1]
© M-Science Group
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CONCLUSION
Integration of engineering principles through the
application of innovative technology utilizing
Endermological® LPG® techniques and the
mechanotransduction of tissue in the process of
targeted personified post-transplantation therapy
provide effective and new therapeutic tools in tissue
and systems regeneration and rehabilitation.
This article was funded by the University of Žilina
project APVV 15-0405 “Complex use of X-ray
diffractometry for identification and quantification of
functional properties of dynamically loaded structural
elements from important technical materials”
Used sources
[1] Mezencevová V., Trebuňová M., Živčák J., Endermo-
lógia a LPG mechanotransdukcia v procese biocom-
patibility rôznych štruktúr titánových implantátov s
mezenchymálnymi kmeňovými líniami, Novus
Scientia, 2015
[2] Živčák, J. a kol.: Základy bioniky a biomechaniky ,
ManaCon 2004: 256 s.
[3] Discher, D.E., Janmey, P., Wang, Y.L.: Tissue cells
feel and respond to the stiffness of their substrate.
Science 2005; 310:1139-1143
[4] Baumann, K.: Adhesion forces promote transcript-
tion , Nature Reviews Molecular Cell Biology 2015;
16, 390-391
[5] Benham-Pyle, B.W., Pruitt, B.L., Nelson,W.J.:
Mechanical strain induces E-cadherin-dependent
Yap1 and β-catenin activation to drive cell cycle
entry. Science 2015; 348:1024-1027
[6] Engler, A.J., Sen, S., Sweeney, H.L., Discher, D.E.:
Matrix elasticity directs stem celllineage specifica-
tion.Cell.2006;126:677-689.
[7] Swift, J., Ivanovska, I.L., Buxboim, A., Harada, T.,
Dingal, P.C.D.P., Pinter, J., Pajerowski, J.D., Spinler,
K.R., Shin J-W., Tewari M. et. al.: Nuclear Lamin-A
Scales with Tissue Stiffness and Enhances Matrix-
Directed Differentiation. Science: 2013; 341, 6149
[8] Pellegrin, S., Mellor, H.: Actin stress fibres. J. Cell
Science: 2007; 120, 3491-3499
[9] Rehfeldt, F., Brown, A.E.X., Raab, M., Cai, S.S.,
Zajac, A.L., Zemel, A., Disher, D.E.: Hyaluronic acid
matrices show matrix stiffness in 2D and 3D dicta-
tes cytoskeletal order and myosin-II phosphory-
lation within stem cells. Integr. Biol.-UK: 2012; 4,
422-430
[10] Yoshikawa, H.Y., Kawano, T., Matsuda, T., Kidoaki,
S., Tanaka, M.: Morphology and Adhesion Strenght
of Myoblast Cells on Photocurable Gelatin under
Native and Non-native Micromechanical Enviro-
ments. J. Phys. Chem. B.: 2013. 117, 4081-4088
[11] Revolution In the treatment of lymphoedemas,
LPG Health Scientific research 2005; 6.
[12] Piller, N.B. et al: Secondary arm lymphoedema
treated with Endermologie®: A randomized trial;
The effectiveness of a new Endermologie-LPG®
treatment program for arm lymphoedema:
Objective and subjective data from case studies.
Internacional Congress of Lymphology; Sep.-Oct.
2005; Bahia, Brazil
[13] Revuz, J. e coll.: Effects cliniques et histologiques
d´un appareil, le Lift 6®, utilize dans le veillisement
du visage. Nouv.Dermatologie 2002; 21:335-342
[14] Lafontan, M.: Use of the microdialysis technique to
assess lipolytic responsiveness of femoral adipose
tissue after 12 sessions of mechanical massage
technique. J Eur Acad Dermatol, Venereol 2008;
1465-1470
[15] Lafontan, M.: Evaluation of the effects of
Endermologie® on lipid mobilization and gene
expression using microdialysis and DNA microarray
analysis respectively. International Congress of
Aesthetic Medicine Moscow Jan. 2010;
[16] Merques M.A., Combes, M., Roussel, B.,Dupont,
L.V., Thalamas, C., Lanfontan, M., Vuguerie, N.:
Impact of a Mechanical Massage ogn Gene
Expression Profile and Lipid Mobilisation in Female
Glutefemora Adipose Tissue, Obesity Facts 2011;
4:121-129
[17] Humbert, P.: Clinical, biometrological and historical
evaluations after mecanichal stimulation on the
face with the Endermolift™ Technique. Innovation -
international convention july 2013
[18] Zauskova, L., Czan, A., Sajgalik, M., Drbul, M.,
Rysava, Z.: Triaxial Measurement of Residual stress
after High Feed Milling Using X-ray Diffraction, In
Proceedian Engineering conference paper, vol.
192, 2017, pp. 982-987, ISSN 1877-7058
[19] Majani, U., Majani, A.: Tissue mechanostimulation
in the treatment of scars, Acta Medica Mediterra-
nea, 2013;29.191-192
[20] Berkovitz, B.K.B.: The Structure of the Periodontal-
Ligament - an Update. Eur J Orthodont 1990; 12:51-
76
[21] Reichenberg, E., Redlich, M., Cancemi, P., Zaks, B.,
Pitaru, S., Fontana, S. a kol. Proteomic analysis of
protein components in periodontal ligament
fibroblasts. J Periodontol 2005; 76:1645-1653
[22] Erickson, H.P., Bourdon, M.A.: Tenascin - an
Extracellular-Matrix Protein Prominent in Speciali-
zed Embryonic-Tissues and Tumors. Ann Rev Cell
Biol 1989; 5:71-92
[23] Chiquet, M., Koch, M., Tannheimer, M., Chiquet-
Ehrismann, R.: Regulation of extracellular matrix
synthesis by mechanical stress. Biochem. Cell Biol.
1996; 74:737-44
[24] Ruoslahti, E.: Fibronectin. J Oral Pathol Med 1981;
10:3-13
[25] Adcock, D., Paulsen, S., Jabour, K., Davis, S.,
Nanney, L.B., Bruce Shack, R.: Analysisi of the
effect of deep mechanical massage in the porcine
model.Plast.Recondtr.Surg.2001;108:233-234
[26] Bacci,P.A.,:Endermologie®-LPG® Systems© after
15 years
[27] Czán,A., Babík,O., Mikloš,M., Zauškova,L. :
Assessment of surface area characteristics of
dental implants with gradual bioactive surface
treatment, TECHNOLOGICAL ENGINEERING, Volume
XII, number 1/2015, ISSN 1336 5967
[28] LPG® Systems:2004,https://www.lpgsystems .com
[29] Babík,O., Czan, A , Holubják,J., Kameník,R., Pilc,J.,:
Non-destructive analysis of basic surface
characteristics of titanium dental implants made by
miniature machining, Technological engineering,
volume XIII, number 2/2016
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