Brewer's yeast, as the main byproduct of the beer industry, has a high value for development and utilization. Here, brewer's yeast was used as the raw material from which an odorless extract was prepared by autolysis and ethyl acetate extraction. Ultraviolet (UV) protection, tyrosinase inhibitory effect, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, hygroscopicity, moisture retention, and other skin care benefits of the extract were studied. The results showed that when the protein mass concentration of the odorless extract of brewer's yeast was 4.77 g/L, the average UV, UVA, UVB, and UVC absorbance, the tyrosinase inhibition rate and DPPH scavenging rate were 85.08%, 64.25%, 93.42%, 100%, 97.90%, and 31.12%, respectively. The hygroscopicity of this extract was significantly higher than that of glycerin, and the moisture-retention capacity of this extract was equivalent to that of glycerin. The results indicate that the odorless yeast extract could serve as a good UVB absorber and tyrosinase inhibitor. It could also prevent sunburn caused by UV absorption, skin cancer, and other associated health problems. It also shows sun screening and whitening effects, antioxidant activity, moisture retention capacity, and other skin care benefits. © 2017, Editorial Board of Modern Food Science and Technology. All right reserved.