Our study aimed to investigate the association between preeclampsia and VEGF +936C/T, -634G/C, -2578C/A and -1154G/A polymorphisms and risk of in the Han Chinese pregnant women. A total of 128 PE cases and 128 normal pregnant women as control were enrolled in a Chinese population. Polymerase chain reaction (PCR) amplification of the genes and sequencing methods were used to genotype VEGF SNPs +936C/T, -634G/C, -2578C/A and -1154G/A. The association between VEGF SNPs +936C/T, -634G/C, -2578C/A and -1154G/A and risk of PE was assessed by the single factor and multiple factors logistic regression. We observed that the TT genotype of VEGF +936C/T was associated with an increased risk of PE when compared with the CC genotype (OR=2.49, 95% CI=1.32-4.68). In recessive model, those carrying the TT genotype of VEGF +936C/T had an strong increased risk of PE in comparison to those with CC+CT genotype (OR=2.43, 95% CI=1.32-4.48). We found the VEGF +936C/T had interaction with smoking and drinking in the risk of PE. In conclusion, the TT genotype of VEGF +936C/T polymorphism have an increased risk of PE, and have an interaction with smoking and drinking. VEGF +936C/T polymorphism could be a susceptibility biomarker for PE.