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Assessment of Nutritional Quality and Global Antioxidant Response of Banana (Musa sp. CV. Nanjangud Rasa Bale) Pseudostem and Flower

  • JSS Academy of Higher Education and Research (JSS AHER) Mysore Karnataka - 570015
  • Terresian College

Abstract and Figures

Background: The assessment of the nutritional composition and phytochemical screening of banana pseudostem (PB) and flower (FB) advocate this nonconventional food source for routine consumption, considering its various health benefits. Objectives: The aim is to assess the proximate nutrient composition, fatty acids, minerals, amino acid profile, and global antioxidant response (GAR) of PB and FB. Methods: Standard analytical procedures were used to determine the nutritional quality and GAR of PB and FB. Results: The chemical analysis illustrated that functional profile (water holding capacity, oil holding capacity, swelling power, and solubility), and proximate (ash, moisture, protein, fat, dietary fiber, and carbohydrate) contents were substantially high in FB than PB. With a well-proportionate amino acid profile, PB (0.56) and FB (0.54) comprised of a high ratio of essential to nonessential amino acids than those of FAO/WHO requirement (0.38). The mineral analysis revealed that PB and FB were rich in macro and micro minerals in the order K > Ca > Mg > P > Na and K > Mg > Na > Ca > P, respectively. Linoleic acid was found to be the major component in PB and FB. Besides, total antioxidant activity conducted for PB and FB by GAR method, measuring both bio-accessible and insoluble fractions, revealed that the soluble fraction fared better than the chemical extracts. Conclusion: The results revealed high nutritional qualities of the byproducts of banana and the low cost of its production promotes their use as a prospective nonconventional food resource with high nutraceutical value. Summary: AOAC: Association of Analytical CommunitiesFAO/WHO: Food and Agriculture Organization of the United Nations/World health organization Abbreviations Used: Banana flower was more potent than banana pseudostem in terms of its nutritional quality and total antioxidant capacity affirming their usefulness (of both the secondary products) in the pharmaceutical sector as a nutritional supplement due to the health-related properties of dietary fibre and associated bioactive compounds.
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December 2017 | Volume 9 | Supplement 1
Pharmacognosy Research Volume 9 Supplement 1 December 2017 Pages XXX-XXX
5.5 mm
S74 © 2017 Pharmacognosy Research | Published by Wolters Kluwer ‑ Medknow
Assessment of Nutritional Quality and Global Antioxidant
Response of Banana(Musa sp. CV. Nanjangud Rasa Bale)
Pseudostem and Flower
Ramith Ramu, Prithvi S. Shirahatti, K. R. Anilakumar1, Shivasharanappa Nayakavadi2, Farhan Zameer3,
B. L. Dhananjaya4, M. N. Nagendra Prasad5
Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College of Post Graduate Centre, Dakshina Kannada, 1Food Quality and Assurance Department,
Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, 3Department of Studies in Biotechnology, Microbiology and Biochemistry, Mahajana Life
Science Research Centre, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, 5Department of Biotechnology, Sri Jayachamarajendra College of Engineering,
Mysore, 4Toxinology/Toxicology and Drug Discovery Unit, Centre For Emerging Technologies, Jain University, Bengaluru, 2Department of Veterinary Pathology,
Animal Science Section, ICAR-Central Coastal Agricultural Research Institute, Ela, Goa, India
Background: The assessment of the nutritional composition and
phytochemical screening of banana pseudostem (PB) and ower (FB)
advocate this nonconventional food source for routine consumption,
considering its various health benets. Objectives: The aim is to assess the
proximate nutrient composition, fatty acids, minerals, amino acid prole,
and global antioxidant response(GAR) of PB and FB. Methods: Standard
analytical procedures were used to determine the nutritional quality and
GAR of PB and FB. Results: The chemical analysis illustrated that functional
prole (water holding capacity, oil holding capacity, swelling power, and
solubility), and proximate (ash, moisture, protein, fat, dietary ber, and
carbohydrate) contents were substantially high in FB than PB. With a
well‑proportionate amino acid prole, PB(0.56) and FB (0.54) comprised
of a high ratio of essential to nonessential amino acids than those of
FAO/WHO requirement (0.38). The mineral analysis revealed that PB and
FB were rich in macro and micro minerals in the order K>Ca>Mg>P
> Na and K>Mg>Na>Ca>P, respectively. Linoleic acid was found to
be the major component in PB and FB. Besides, total antioxidant activity
conducted for PB and FB by GAR method, measuring both bio‑accessible
and insoluble fractions, revealed that the soluble fraction fared better than
the chemical extracts. Conclusion: The results revealed high nutritional
qualities of the byproducts of banana and the low cost of its production
promotes their use as a prospective nonconventional food resource with
high nutraceutical value.
Key words: Amino acid, fatty acid, global antioxidant response, mineral
element, proximate composition
•  AOAC: Association of Analytical Communities
•  FAO/WHO: Food and Agriculture Organization of the United Nations/World
health organization
Abbreviations Used: Banana ower was more potent than banana
pseudostem in terms of its nutritional quality and total antioxidant
capacity afrming their usefulness (of both the secondary products) in the
pharmaceutical sector as a nutritional supplement due to the health‑related
properties of dietary fibre and associated bioactive compounds.
Dr.M. N. Nagendra Prasad,
Department of Biotechnology,
Sri Jayachamarajendra College of Engineering,
JSS Institution Camp, Manasagangothri,
Mysore‑570006, Karnataka, India.
DOI: 10.4103/pr.pr_67_17
e population explosion has exemplied a substantial rise in the
demand for food resources which has shied the attention of the global
food market to nonconventional food resources.[1] Several by‑products
of cultivation, once discarded as wastes, are studied for their nutritive
values to advocate their use as routine food sources meeting the
expanding demands of the industry. Such by‑productshave proved to be
economical and hence are well‑accepted in the global market considering
the present scenario.[2] India contributes a major portion of the total
banana production in the world, whereas it is a conventional form of
food and commercial food as well. Banana cultivation comprises of the
secondary products, banana pseudostem(PB), and banana ower(FB)
which have been discarded as wastes, fed to cattle or used for composting.
More recently, some studies have reported its use in the production of
alcohol, methane, food for livestock, or adsorbents for water purification.
Amassive quantity(about 40%) of the total fresh weight of banana plant
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Cite this article as: Ramu R, Shirahatti PS, Anilakumar KR, Nayakavadi S,
Zameer F, Dhananjaya BL, et al. Assessment of nutritional quality and global
antioxidant response of banana (Musa sp. CV. Nanjangud Rasa Bale) pseudostem
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RAMITH RAMU, et al.: Nutritional Quality of Banana Flower and Pseudostem
Pharmacognosy Research, Volume 9, Supplement 1, December 2017 S75
comprises of PB and FB, and hence, it can be useful as an alternative
food resource.[3] However, to accomplish the potential health benets of
these, a detailed study of its nutritional value needs to be carried out with
special emphasis on the inuence of a particular variety on its nutritional
composition. In vitro and invivo studies using the extracts have proven
PB and FB as antihyperglycemic,[4,5] antimicrobial, hypolipidemic,
and anti‑hypertensive agents thus upholding their health benecial
properties.[6] e dietary ber content, antioxidant compounds and
several other macro‑ and micro‑nutrients are responsible for these
health benets, and thus, the present study was designed to assess the
composition of PB and FB in terms of its nutritional value.
Further, it is well‑known that diseases either acute or chronic induce the
generation of reactive oxygen species which are also the main factors
responsible for tissue damage and aging.[7] To ameliorate these damages,
a mode of treatment which also has antioxidant properties has improved
the condition and hence, this study also aims to evaluate the antioxidant
potential of PB and FB. Several procedures are available to assess the
antioxidant properties of food, and its potency depends upon either the
method of assessment or the method of extraction of samples. In this
regard, the total antioxidant capacity(TAC) can be determined aer
analyzing the mode of action likely to be either radical scavenging or
metal ion chelating activity.[8] In addition, the extraction procedure
plays a signicant role for its TAC since irrespective of the extraction
procedure; some fraction of the extract always remains insoluble and
not involved in the activity. More recently, a method developed by
Vural etal.[9] known as Quencher is being widely accepted for TAC
evaluation since the method is carried out without extraction and
thus the entire sample is tested in its solid state. e acceptance of this
method is also armed because it resembles the condition similar to
the physiological conditions where antioxidants are not extracted and
administered directly, but instead, it needs to be released from the food
source during digestion. Similar to the physiological conditions, aer the
enzymatic digestion, the extractable antioxidants play their role, and the
nonextracted materials enter the intestine where they are acted on by the
intestinal microora and continue the digestion process. e extracted
antioxidants are estimated by conventional methods, and the undigested
material is evaluated for its antioxidant capacity using the Quencher
method thus attaining athe global antioxidant response(GAR). is
method provides a summation of the complete antioxidant potential of
the food source the same way as it exists invivo and hence, the method
is widely accepted.[10]
With this background, the objectives of this study are an evaluation of
the nutritional composition and TAC of PB and FB to advocate this
nonconventional food source for routine consumption, considering its
various health benets.
Flawless inorescences and pseudostems of Musa sp. cv. Nanjangud rasa
bale were harvested from the banana cultivatingfarms of Nanjangud,
Karnataka, India. e specimen was identied by the Department
of Horticulture, Government of Karnataka, Mysore, India. Peeling
the thick outer leaf‑sheath of the tender pseudostems, the inner pith
region was collected and owers were separated from the inorescences
by discarding the spathe. For isolation, pseudostems (PB) and FBs
were gutted, chopped and allowed to dry in an oven(40⁰C). is was
pulverized, using a homogenizer and further stored at 4°C until use.
Proximate analysis
Moisture (method 44‑15A), ash (method 08–01), crude
ber (method 32–10), fat (method 30–25), and carbohydrate
content of PB and FB were determined according to the AACC
method.[11] Total carbohydrates were expressed as residual percent weight
by the formula:[100‑(moisture + ash+ fat + bre+ protein)]. Crude
protein (method 46–13) was estimated by the procedure described
by Kjeldahl.[12] e total dietary ber content(method 991.43) in PB
and FB was estimated by food‑enzymatic‑gravimetric method.[13] e
procedure described by omas etal.[14] was used to determine neutral
detergent ber(NDF), acid detergent ber(ADF), lignin, hemicellulose,
and cellulose content in PB and FB. Hemicellulose and cellulose were
estimated according to the formula: [Hemicellulose = NDF– ADF];
and [Cellulose = ADF– lignin], respectively. Water holding
capacity (WHC), oil holding capacity (OHC), swelling power (g of
swollen granules/g of dry weight of sample), and solubility(%) of PB and
FB were performed as per the method described by Noor etal.[15] WHC
and OHC were expressed as grams of water or oil/grams of dry weight
of samples, respectively. Starch and uronic acid content were determined
by the method described by Jamuna etal.[16] e phytochemical analysis
and the vitamin content of PB and FB were determined.[17‑19] e sugar
composition was performed according to AACC[20] for PB and FB using
high‑performance liquid chromatography (HPLC) with dierential
refractive index detector(RID‑10A, Shimadzu, Japan).
Fatty acid prole by gas chromatography‑mass
Before gas chromatography‑mass spectrometry (GC‑MS) analysis,
derivatization was performed for PB and FB samples using BF3‑methanol
as derivatizing reagent.[21] Once the conversion of non‑volatile fatty acids
into volatile fatty acid methyl esters(FAMEs) through methylation[22] was
achieved, the samples were subjected to GC(Clarus 500, Perkin Elmer,
AOC‑20i autosampler; Perkin Elmer, California, USA) interfaced with
a mass spectrometer equipped with an Elite‑5MS(5% diphenyl/95%
dimethyl polysiloxane) fused to a capillary column(30nm× 0.25mm
ID×0.25 μm film thickness, DF). To achieve a good resolution of FAMEs,
chromatographic parameters were optimized as per Ramith etal.[5] with
slight modications in the oven temperature. It was programmed at the
rate of 10°C/min(no hold) up to 200°C, later at the rate of 5°C/min up
to 280°C for a 9min hold. In comparison to the acquired mass spectra
of PB and FB with the standard mass spectra of NIST Library(NIST 05),
the phytocomponents present were recognized.
Mineral analysis
PB and FB were assessed for comprehensive mineral analysis(Li, B, Na,
Mg, Al, K, Ca, Cr, Mn, Fe, Cu, Ni, Cs, Zn, and Pb) using inductively
coupled plasma atomic emission spectrometry(ICP‑AES, Varian Vista
MPX, USA) as per the official method 985.01.[23] Ahead of subjecting to
ICP‑AES, the dry samples were ashed in a muffle furnace at 400°C–500°C
and acid digested.[23,24] On the other hand, the concentrations of Mo,
Se, P, As, Cd, and Sb were determined using flame atomic absorption
spectroscopy(AAS, Varian 240, USA) according to the method described
by Vikas etal.[25] Phosphoric acid and boric acid were measured
according to the method described by Pearson[26] and method 970.33.[27]
e elemental analysis of PB and FB was performed on a Perkin Elmer
2400 elemental analyzer.
Amino acid composition
Amino acid composition of PB and FB was analyzed according to
the standard AOAC procedure (method 994.12).[28] For hydrolysis,
methods of Wong and Peter[29] were employed. Before the analysis of
the samples through automated amino acid analyzer(L 8900, Hitachi,
Japan), ltration was performed using a 0.45mm nylon membrane lter.
Subsequently, following the prehydrolysis oxidation with performic
RAMITH RAMU, et al.: Nutritional Quality of Banana Flower and Pseudostem
S76 Pharmacognosy Research, Volume 9, Supplement 1, December 2017
by the ltering of individual extracts from solvents. e three ltrates
were then stored at−20°C until used for the analysis of total phenolic
content and antioxidant activity. All the samples were analyzed in
triplicates. e phenolic component separation of PB and FB extracts
was performed on a reverse phase C18(250mm×4.6mm, Supelco) and
the compounds were monitored by PDA (photodiode array) detector
HPLC system(Agilent Technologies Inc., USA). Column temperature
was maintained at 37°C and ow rate was set to 0.8ml/min. e solvent
system used was 0.1% formic acid(solvent A) and methanol(solvent B).
e solvent gradient elution program was: 0–55min 85% of A and 15%
of B; 55–57min 20% of A and 80% of B; 57–60min 85% of A and 15%
of B. Avolume of 20 μl of the sample was injected(auto‑injection) into
the column and the phenolic acids were detected at 280nm. e sample
was quantied by comparing the retention time/peak areas with those of
standards, namely, gallic acid, p‑hydroxybenzoic acid, chlorogenic acid,
sinapic acid, caeic acid, vanillin, p‑coumaric acid, quercetin, catechin,
and epicatechin. e Quencher procedure described by Vural etal.[9]
was employed to determine antioxidant activity of the solid sample. In
addition, enzymatic dismutase (SOD), ascorbate peroxidase (APX),
glutathione reductase (GR), and catalase (CAT) activities were
determined by following the method of Moaed etal.[33] and Manoj etal.[1]
Statistical analysis
All data were expressed as mean±standard deviation(n=3). Results
were determined using one‑way analysis of variance(ANOVA), followed
by Duncan’s multiple range test using SPSS Soware (version 21.0,
Chicago, USA). e results were considered as statistically signicant if
the P<0.05.
acid, cysteine, and methionine (sulfur‑containing amino acids) were
determined.[30] In comparison with the FAO/WHO(1985)[31] reference
amino acid pattern, the composition of dierent amino acids recovered
was presented as mg/g of protein. e essential amino acid(EAA) score
was evaluated using the equation of FAO/WHO described: (Score of
EAA=mg of EAA in 1g of test protein/mg of EAA in 1g of reference
protein) × 100.
Antioxidant potential
To measure antioxidants in PB and FB, dierent procedures were
established using (i) GAR method (GAR); (ii) Sequential solvent
extraction method and(iii) without extraction. In GAR, physiological
extraction by the means of invitro gastrointestinal digestion was done as
described by Pastoriza etal.[7] e invitro GAR method is performed to
mimic digestion(through gastrointestinal tract) to discharge antioxidants
from foods into soluble(bio‑accessible) and insoluble(nonaccessible)
fractions, which is summarized in Figure1. ree diverse conventional
protocols(DPPH, ABTS, and FRAP) dened by Cristina etal.[32] for the
soluble fractions obtained by gastrointestinal digestion was followed.
In all cases, the results were expressed as mmol equivalents of Trolox
per kg of sample.[32] To determine the antioxidant activity of lyophilized
insoluble fractions obtained by gastrointestinal digestion, the Quencher
procedure was conducted as described by Vural etal.[9] Calibration curve
was obtained using Trolox solutions and microcrystalline cellulose as
the blank. Results were expressed as mmol equivalents of Trolox per
kg of sample. Second the coarse powder was subjected to successive
extraction with methanol, ethanol and water in a Soxhlet apparatus.
Extraction was done twice with each of the solvents(500ml) followed
Figure 1: A procedure to determine global antioxidant response of banana pseudostem and ower
RAMITH RAMU, et al.: Nutritional Quality of Banana Flower and Pseudostem
Pharmacognosy Research, Volume 9, Supplement 1, December 2017 S77
Dietary ber composition
e proximate composition of PB and FB are dened in Table1 which
suggests a high total dietary ber content. Adiet comprising of high
dietary ber is ecient in generating early satiety signal by increasing
the food retention time in the stomach and also reduces risk towards
the development of gastric ulcers. Of the total dietary bers, while
the soluble bers possess the property of higher expansion volume
rendering bulk density of the food materials, insoluble bers swell on
encounter with water promoting the elimination of waste materials by
increasing bowel movement. us, a ber‑rich diet facilitates digestion,
as well as elimination of wastes and also prevents constipation.[34] In
this study, the IDF was found to be more than the soluble dietary
ber in both the byproducts tested and on the whole, dietary ber in
FB(70.1%) was found to be higher than PB(61.1%). In support to the
present ndings, a previous study suggested IDF as the major fraction in
the dietary ber composition of banana(Musa acuminata×balbisiana
Colla cv. Awak) pseudostem flour and boiled tender core of the PB
flour.[15] As well, a similar trend was observed in banana and plantain
peels.[14] In summary, despite the dierence in the ber content in
comparison to other studies, the dietary ber content of PB from Musa
sp. cv. Nanjangud rasa bale was higher than PB from Musa sp. cv. elakki
bale, which had a value of 28.8%.[16] Such dierences may be attributed
to the dierent botanical origins, geographical conditions such as soil,
climate, and collection time. ese results indicate the potential of
banana by‑products soon to replace oats and sorghum as berenriched
food source.
Contrary to Insoluble dietary ber (IDF), NDF includes a complex of
cellulose, lignin and insoluble hemicellulose. Accordingly, NDF values
are always higher than those of the IDF and present study results support
this as it is clear from Table1. ADF and NDF content of FB(58.78 and
75.61, respectively) was more than PB(51.88 and 61.25, respectively)
while both were higher than Musa acuminata x balbisiana Colla cv.
Awak pseudostem tender core our(ADF: 32.02; NDF: 43.89) reported
by omas etal.[14] e present study shows that cellulose was the most
abundant component, followed by hemicellulose and then lignin in both
PB and FB. ese components are considered insoluble and thus are not
digested. Overall, the relative amounts of hemicellulose and cellulose in
the study were higher than those published by Samrat etal.[35](14.98%
hemicellulose and 31.27% cellulose) and omas etal.[14](18%
hemicellulose and 42% cellulose) PB fibers of Musa sapientum andMusa
acuminata x balbisiana Colla cv. Awak, respectively. Cellulose constitutes
the major component or primary and secondary cell walls, thus
explaining their presence at high levels. However, the cellulose content
of PB and FB reported in this study is less than the cellulose content in
the outer bark material of pseudostems of M. acuminate Colla(40.2%)
and pseudostem tender core our as reported by Cordeiro etal.[36] and
omas etal.[14] respectively. On the contrary, the lignin content of both
PB and FB was higher than banana pulp(6.0%), wheat(0.88%), and soy
meal(0.58%), as reported by Jrgen[37] and are considerably lower with
other wood‑based materials such as sawdust(20.33%), Musa sapientum
species(15.07%) and plantain(green banana)(14.3%).
Functional properties
e functional properties such as WHC, OHC, solubility, and swelling
capacity(SWC) of banana by‑products were measured and are presented
in Table1. From the physiological standpoint, the ability of any material
to retain water when subjected to an external centrifugal gravity force
or compression is its WHC. e study suggests that FB exhibited
highest WHC (23.9 g water/g dry weight of samples) compared to
PB (15.4 g water/g of dry weight of samples). Meanwhile, PB and FB
exhibited greater WHC than those of cereals which showed<5.5g water/g,
such as rice bran(5.21g water/g) and durum wheat(1.5–2.1g water/g).
is minute disparity may be due to the structural dierences in cell wall
components between the stem and ower fibers. Subsequently, the SWC
of PB and FB were assessed which is directly attributed to the amount of
cellulose in the dietary ber. e extent of water retained in the swollen
granules of FB(16.02g of swollen granules/g of dry weight of sample) was
significantly(P<0.05) greater than PB(12.58g of swollen granules/g of
dry weight of sample) with no statistically significant(P<0.05) dierences
in solubility between them, suggesting them to be more potent than some
of the exotic fruits such as pineapple and mango concentrates(7.2, 6.6
and 4.60ml water/g sample, respectively). Furthermore, the OHC of PB
and FB were assessed which is attributed to the chemical and physical
Table1: Nutritional composition of banana pseudostem and banana ower
Proximate analysis
Moisture(%) 12.30±0.87b8.33±0.79a
Ash(%) 4.93±1.42a6.51±1.05b
Fat(%) 0.98±3.27a5.79±1.78b
Total carbohydrates(%) 46.58±2.33a53.78±6.58a
Starch(%) 21.06±0.87b0.61±0.79a
Energy 64.40±1.25b63.20±0.86a
Uronic acid(%) 31.87±3.55b27.72±4.10a
Protein(%) 7.34±3.60a19.60±5.08b
Total dietary ber(%) 61.14±0.34a70.07±0.25b
Insoluble dietary ber(%) 59.10±0.99a62.93±1.01b
Soluble dietary ber(%) 02.04±0.28a07.14±0.56b
Neutral detergent ber(%) 66.25±0.67a75.61±1.43b
Acid detergent ber(%) 51.88±2.35a58.78±3.03b
Cellulose(%) 44.02±0.91a47.30±0.87a
Hemicellulose(%) 24.37±1.57a16.83±1.13b
Lignin(%) 07.86±0.56a11.48±0.37b
Water holding capacity 15.40±3.90a23.95±2.72b
Oil holding capacity 04.75±4.33a08.00±2.50b
Solubility 12.52±2.07a13.08±1.98a
Swelling power 12.58±1.67a16.02±1.24b
Phytochemical constituents(mg/100 g)
Phenols 188.64±0.88a201.12±1.05b
Flavonoids 78.60±1.18a83.49±0.61b
Alkaloids 62.32±0.39a71.09±0.48a
Tannins 07.86±0.21a86.87±2.43b
Saponins 305.45±0.60a387.51±1.79a
Oxalates 25.56±0.51a20.54±2.08a
Phytates 34.56±3.85a28.78±2.72a
Vitamin(mg/100 g)
Ascorbic acid 8.81±0.20a9.50±0.05b
Riboavin 0.08±0.18a0.13±0.07b
Niacin 0.73±0.19a0.90±0.27b
iamine 0.15±0.06a0.18±0.04b
β‑Carotene 0.08±0.24a0.12±0.16b
Vitamin E 0.12±0.04a0.17±0.12b
Pyridoxine 0.33±0.16b0.28±0.04a
Pantothenic acid 0.34±0.08b0.26±0.20a
Sugar composition(mg/kg)
Fructose 0.01±0.44a4.43±0.08b
Glucose 0.01±0.43a5.33±0.14b
Sucrose 10.27±0.02b7.74±0.06a
Maltose 0.02±0.08a9.38±0.30
Xylose 0.04±0.12b0.02±0.18a
Arabinose 10.60±0.20 1.53±0.10a
Rhamnose 0.62±0.25b0.05±0.08a
Values are expressed as mean±SD(n=3). Means in the same row with distinct
superscripts are significantly dierent(P≤0.05) as separated by Duncan multiple
range test. PB: Banana pseudostem; FB: Banana ower; SD: Standard deviation
RAMITH RAMU, et al.: Nutritional Quality of Banana Flower and Pseudostem
S78 Pharmacognosy Research, Volume 9, Supplement 1, December 2017
structure of the plant polysaccharides. With an OHC value of 4.75 of
oil/g of dry weight in PB and 8.0g of oil/g of dry weight of sample in
FB, they fared better than dietary fibres obtained from commercial
preparations(1.29 g of oil/g of dry matter) and other fibrous residues,
such as coconut fibre (5.3 g oil/g fibre or banana fibre‑rich powder
(2.2g oil/g fibre). With these results, PB and FB can be advocated for use
in stabilizing emulsions and as a dietary ber reservoir.[14,38]
Sugars are the main source of bio‑available energy, and hence, it is
important to assess the sugar content as well as the type of sugars present
in the food. e digestible sugar content in PB was 21.57mg/kg, and
FB was 28.48mg/kg samples[Ta b l e 1] and was not as high as banana
fruit and other tropical fruits.[18] Further, the sugar prole of PB revealed
the presence of sucrose and arabinose, which contributed 47.6% and
49.1% to total sugars, respectively while FB revealed the presence of
several types of sugars, namely, maltose, sucrose, glucose, fructose, and
arabinose. Despite the quick metabolism of these sugars, their presence
at low levels prevents the use of PB and FB as an alternative energy
resource. While the present study revealed the absence of galactose and
rhamnose, a previous GC‑MS study of polysaccharide fractions of Musa
sp. cv. elakki bale suggested their presence[16] which might be due to the
dierence in the banana cultivars used in the study. In addition, the level
of sucrose was higher than glucose in both PB and FB. Other studies
with banana peel exhibited high fructose while banana pulp showed the
presence of glucose, fructose, and sucrose with lower sucrose levels as
compared to other sugars.[39]
A class of alkaloids, flavonoids, tannins, saponins and more complex
phenolic, phytosterols, oxalates, and phytates are collectively known as
phytochemicals which not only impart color to the fruits and vegetables
but also possess several physiological functions, including antioxidant
properties.[40] Table1 elaborates on the phytochemicals and their amount
in PB and FB which reveal that the most abundant phytochemical
in this study are phenols and saponins. Furthermore, high tannin
content(86.9mg/100g) in FB as comp ared to PB was witnessed. All these
phytochemicals are proven to possess antimicrobial, antioxidant, and
hormone modulatory activities. e study also revealed high amounts
of avonoids, which are well‑known for their antioxidant properties.
e higher avonoids and saponins were present in PB and FB than in
banana owers of two cultivars[41] Baxijiao(saponins: 0.11 g/100g and
avonoids: 5.90mg/100g) and Paradisiacal(saponins: 0.12 g/100g and
avonoids: 5.27. mg/100g) and considering these benets, the potential
of PB and FB for their health beneciary properties is upheld.
Vitamins are the micronutrients required in minute amounts to the
body, deciency of which adversely aect the metabolism of the body.
In the present study, Vitamin C (ascorbic acid) was present in the
highest quantity with a mean content of 9.50 and 8.81mg/100g of FB
and PB, respectively. While ascorbic acid is among the most important
antioxidants involved in the prevention or minimization of the formation
of carcinogenic substances from dietary material by preventing the
oxidation of nitrate, its deciency causes impaired functioning of the
intracellular substances in the body including collagen, bone matrix, and
tooth dentine.[40] In addition to ascorbic acid, riboavin, niacin, thiamine,
β‑carotene, vitamin E, pyridoxine, and pantothenic acid were observed
in PB and FB in quantities signicant to create a nutritional impact by the
food source[Table1]. Vitamin C content in FB and PB was lower than
banana fruit(10mg/100g) but higher than other tropical fruits, namely,
blueberries(6mg/100g) pears(3mg/100g), and grapes(3mg/100g).
Furthermore, the vitamin B complex was present in a signicant amount
which emphasizes these by‑products for their potential in the treatment
of various diseases including prostate cancer.[18]
Fatty acids
Fatty acid composition as given in Tab l e 2 suggests that linoleic acid
and palmitic acid were the major components in both the parts of the
banana. FB contained 84.8% linoleic acid of its total fatty acid content,
while PB contained 72.8%, which was followed by palmitic acid, which
was high in PB (18.9%) compared to FB (14.8%). ese results were
similar to banana fruit peels of the Musa Genus: French Clair (FC),
Grande Naine (GN), Big Ebanga (BE), pelipita (PPT), Yankambi
Km5(YKm5), and CRBP039039 had high proportions of unsaturated
fatty acid, especially linoleic acid.[40] Linoleic acid is a precursor fatty acid
for cell membrane components as well as other compounds involved in
physiological responses and its presence in this study proves benecial.
Further, some of the less common fatty acids in PB were stearic acid and
arachidic acid and FB was eicosenoic acid. Further, the polyunsaturated
fatty acid levels were greater in this study as against Musa spp. Baxijiao
and Paradisiacal owers.[41] Such variations may be attributed to the stage
of ripening at harvest, changes in the climate, soil conditions, and genetic
variations between the sources. e online Dr.Duke’s phytochemical and
ethnobotanical database‑assisted in ascertaining the biological activity
of the compounds and the same are tabulated in Table 2.
Based on the amount required for the human body, minerals are classied
as macro‑and micro‑elements. e minerals present in PB and FB are
given in Tab l e 3 which suggests the presence of Na, K, Ca, Mg, and P with
K being the major mineral in both PB and FB. However, minerals in FB
were about 2–5‑fold higher than the levels in PB. e levels of minerals
in FB were in the order K>Mg>Na>Ca>P while that of PB was in
the order K>Ca>Mg>P > Na. While Na and K are involved in the ion
pumps in several metabolic pathways, Mg regulates over300 metabolic
reactions by acting as cofactors to several enzymes, P is involved in almost
every chemical reaction taking place in the body in the form of ATP and
Ca along with P forms Ca3(PO4) 2 and are essential for bone and teeth
formation.[42] Overall, the levels of these minerals were in agreement
with that of Musa spp. Baxijiao and Paradisiacal ower variety,[41] but
slightly lower than the limiting contents found in banana peels and pulps
determined by Shaida etal.[43] In summary, the peel had a higher content
of minerals than the pulp and the potassium content was lower to banana
fruits and other tropical fruits such as pears, blueberries, and grapes.[18]
Along with these macro minerals, the micro minerals in PB and FB were
also evaluated which showed the presence of Fe, Mn, Zn, Cu, Al, and
several others are listed in Table 3. e levels of these elements in FB were
found to be higher than PB and overall higher than those of other tropical
fruits, including banana when compared with the data given by e
Department of Health(2013).[18] Similar to the various macro‑elements,
microelements also have several vital biological functions. Zn is
involved in various reactions of the body to construct and maintain
DNA, required for the growth and repair of body tissues and iron along
with manganese, copper, and zinc are constituents of various important
proteins and enzymes involved in macro‑nutrient metabolism and body
function.[42] Considering the several vital functions of the macro‑and
micro‑elements, their high contents in FB and PB could contribute to
explain their use in folk medicine.
Further, a thorough analysis to evaluate the elements present in the
banana byproducts was performed, and the results are tabulated in
Tabl e 3. Carbon was present in the highest amounts in FB (47.1%)
RAMITH RAMU, et al.: Nutritional Quality of Banana Flower and Pseudostem
Pharmacognosy Research, Volume 9, Supplement 1, December 2017 S79
compared to PB(35.5%) which was contrary to the hydrogen content
which was higher in PB(6.01±0.02) as against FB(3.21±0.02). e
present ndings were on par with the composition of principal elements
of banana(Musa acuminate) pseudostem by Ketty etal.,[44] i. e.,(carbon:
36.83%, hydrogen: 5.19%, and nitrogen: 0.93%). e composition of
hydrogen was higher in PB than FB and this is due to the high moisture
composition of that compared to the FB[Tabl e 1]. e moisture content
of the present study was higher in case of PB(13.3%) over FB(8.33%)
suggesting the dierence in the hydrogen content of both the byproducts.
Overall, the moisture content of both PB and FB were lower than
commercial wheat flour, which had a value of 12.36%[44] and PB and FB
of elakki bale cultivar as reported by Jamuna etal.[16]
In support to the above ndings, the ash content that is directly
proportional to the mineral content was also estimated which suggested
the presence of it at high levels. It is clear from our studies that the highest
levels of ash content were recorded for Musa sp. cv. Nanjangud rasa
bale(PB and FB of 4.9 and 6.5%, respectively), and was comparatively
higher than those Musa sp. cv. elakki bale(0.3 and 0.5%, respectively),[16]
banana fruit of 1.1%.[18] Whereas it was comparable with banana
(Musa acuminata x balbisiana Colla cv. Awak) pseudostem flour(3.03%)
[15] and lower than banana peel and pulps(6.4%–12.8%).[39]
Amino acids
e quality of EAAs suggests the nutritional value of dietary proteins,
and hence the amino acid content in PB and FB were tested. An overall
picture of the amino acid content present in PB and FB is given in
Tabl e4 suggest that all the EAAs according to the FAO classication[31]
are present in them with FB having a major amino acid content
compared to PB. Ahigh glutamic acid content(63.8 and 152.9mg/g
of protein) followed by aspartic acid, leucine, alanine, proline,
arginine, cysteine, serine, and lysine was witnessed in both FB and PB,
respectively. e importance of glutamine is learnt during critical illness
where it acts as a prime carrier of ammonia to the splanchnic area and
the immune system. In addition while the sulfur‑containing amino
acids were above the FAO/WHO,[31] requirement(score ranged from
99 to 240), the other EAAs met FAO/WHO,[31] requirement pattern.
Further, the concentration of the amino acids that are lower than the
FAO standard protein value is considered as limiting concentration,
and in this context, in the present study, lysine was at the limiting
concentration and the same has been reported by omas etal.[39]
Table2: Fatty acid prole of banana pseudostem and banana ower by gas chromatography‑mass spectrometry
Compounds detectedaTrivial name of fatty acid Total percentage composition Activityb
Hexadecanoic acid, methyl ester Palmitic acidx18.93 Lubricant, 5 alpha reductase inhibitor,
antiandrogenic and antioxidants
9,12‑octadecadienoic acid(Z, Z)‑, methyl ester Linoleic acidy72.85 Antiarthritic, anti‑inammatory,
hepatoprotective, hypocholesterol and
5 alpha reductase inhibitor
Octadecanoic acid, methyl ester Stearic acidx6.80 Cosmetics, lubricant, avor,
hypocholesterol and 5 alpha reductase
9‑octadecanoic acid(Z)‑, methyl ester Oleic acidz0.47 Cancer‑preventive, avor,
hypocholesterol and anti‑inammatory
Eicosanoic acid, methyl ester Arachidic acidx0.94 **
Hexadecanoic acid, methyl ester Palmitic acidx14.89 Lubricant, 5 alpha reductase inhibitor,
antiandrogenic and antioxidants
9,12‑octadecadienoic acid(Z, Z)‑, methyl ester Linoleic acidy84.84 Antiarthritic, anti‑inammatory,
hepatoprotective, hypocholesterol and
5 alpha reductase inhibitor
11‑eicosenoic acid, methyl ester Eicosenoic acidx0.27 **
aCompounds were identied by referring to NIST05 library; bActivities were acknowledged by Dr Duke’s phytochemical and ethnobotanical databases; **Activity not
reported; xSaturated fatty acid; yPolyunsaturated omega‑6 fatty acid; zMonounsaturated omega‑9 fatty acid. PB: Banana pseudo stem; FB: Banana ower
Table3: Mineral composition of banana pseudostem and banana ower
Sodium(Na) 0.02±0.02a18.34±0.12b
Potassium(K) 10.63±0.10a51.29±0.04b
Calcium(Ca) 4.01±0.07a10.65±0.05b
Magnesium(Mg) 1.55±0.18a23.55±0.21b
Phosphorus(P) 2.09±0.04a4.10±0.16b
Iron(Fe) 30.65±0.16a405.50±0.04b
Lithium(Li) 0.012±0.01a0.034±0.01b
Boron(B) 39.88±0.04b34.53±0.01a
Aluminium(Al) 7.67±0.01a18.43±0.02b
Chromium(Cr) 5.04±0.04b3.93±0.02a
Manganese(Mn) 27.86±0.09a133.80±0.06b
Copper(Cu) 0.02±0.01a0.52±0.03b
Nickel(Ni) 0.46±0.04a0.99±0.02b
Cobalt(Co) 3.79±0.01a19.44±0.04b
Zinc(Zn) 16.60±0.01a207.90±0.10b
Lead(Pb) 0.15±0.01a0.42±0.02b
Molybdenum(Mo) 0.028±0.01a0.042±0.01b
Antimony(Sb) <0.01 <0.01
Cadmium(Cd) 1.06±0.01a1.50±0.01b
Arsenic(As) 0.015±0.01a0.016±0.01a
Selenium(Se) 0.010±0.01a0.010±0.01a
Boric acid 66.66±0.02a150.0±0.01b
Elemental analysis(%)
C 35.53±0.07a47.19±0.02b
H 6.01±0.02b3.21±0.02a
N 1.35±0.01a1.96±0.03b
S 0.07±0.01a0.17±0.01a
O 52.78±0.06b43.08±0.04a
Values are expressed as mean±SD(n=3). Means in the same row with distinct
superscripts are significantly dierent(P≤0.05) as separated by Duncan multiple
range test. SD: Standard deviation; PB: Banana pseudo stem; FB: Banana ower
RAMITH RAMU, et al.: Nutritional Quality of Banana Flower and Pseudostem
S80 Pharmacognosy Research, Volume 9, Supplement 1, December 2017
in fruit peels of the Musa Genus: FC, GN, BE, PPT, YKm5, and 039,
obtained at three dierent stages of ripeness, namely, stage 1(Green),
stage 5(More yellow than green), stage7(yellow/a few brown spots).
e ratio of essential to non‑EAAs for PB and FB were 0.56 and 0.54,
respectively, which was substantially higher than their requirement in
adults(0.38) as recommended by the WHO. In addition, the protein
values of PB(7.3%) and FB(19.3%) in the present study were marginally
higher than the values reported for Musa spp. Baxijiao and Paradisiaca
owers (1.62%–2.7%), elakki bale cultivar (PB: 2.5 and FB: 12.5%),
banana fruit peels(ranged from 8.3%–10.2%), banana(Musa acuminata
x balbisiana Colla cv. Awak) pseudostem flour(0.89%–3.52%), banana
peels of yelakki bale(7.7%), pachabale(6.7%) and nendrabale(4.6%)
and green banana Cavendish(AAA) our (4.1%). Proteins being the
source for the supplementation of amino acids, it can thus be suggested
that PB and FB are potent sources of EAAs.[3,14‑16]
Antioxidant adjuncts have proven beneciary in many diseases where
they play a protective role in the prevention of ROS mediated damage
to the cells and tissues. Hence, in the present study, we have evaluated
the antioxidant potential of PB and FB and the results thus obtained
are tabulated in Table5a. Studies have suggested both PB[45] and FB[4,16]
of banana as potent antioxidants extractable with aqueous and organic
solvents. Most antioxidant studies involve its evaluation using the
extractable form which creates a lacuna in assessing the nonextractable
substance for their antioxidant capacity and hence, we evaluated
the GAR according to Pastoriza etal.[7] Further, antioxidant activity
using a single assay does not give conclusive evidence hence, three
common radical scavenging assays namely ABTS which determines
the single electron‑transfer capabilities, DPPH which evaluates the
hydrogen‑donating potency and Fe + 3 (FRAP) which reflects the
reductive antioxidant power of the antioxidant compounds[9] were
carried out to assess inv itro antioxidant activity of PB and FB [Figure 5b].
As mentioned previously, along with the method of evaluation, another
factor contributing to the antioxidant potential of the samples is the
method of extraction and hence, a conventional solvent extraction(with
dierent solvents), a direct measure using the QUENCHER procedure,
an invitro gastrointestinal digestion, and the combination of the latter
with the application of the QUENCHER procedure[10] to the insoluble
fraction [termed henceforth the GAR method] are the methods of
extraction employed in the present study. e results provide promising
evidence for the need for employing such methods of antioxidant
estimation since the chemical extraction method(solvents and aqueous)
gave lower results, ranging from 2 to 2.5times lower in comparison with
the Quencher and GAR methods. ey are in agreement with the previous
reports for 27 fresh and cooked foods, estimated by Pastoriza etal.[7] On
the other hand, with regard to the chemical extraction method, both PB
and FB extracted with the solvent ethanol fared better than methanol
and aqueous counterparts. ey were also higher than the Quencher
method, but lower than GAR method of antioxidant evaluation. e
previous phytochemical analysis also reports high amounts of total
phenolic content in the ethanolic extract of PB and FB[4,5] which are
well‑known as the major phytochemicals(phenolic acids and flavonoids)
to possess antioxidant activities in fruits and vegetables.
Further, to acquire a detailed phenolic composition of the extracts,
HPLC analysis was performed and the results are detailed in Table5c
suggesting the presence of diverse phenolic acids, namely, gallic acid,
p‑hydroxybenzoic acid, chlorogenic acid, sinapic acid, caeic acid,
Table4: Amino acid prole of banana pseudostem and banana ower
Amino acids Content(mg/g protein) Reference(mg/g protein)xScore(%)
Leucine 27.2a63.2b66c41 96
Phenylalanine + tyrosine 20.2a55.6b63c32 88
Lysine 12.5a40.0b58c22 69
Valine 10.4a36.6c35b30 105
reonine 9.4a33.0b34b28 97
Isoleucine 11.1a28.0b28b40 100
Methionine + cysteine 24.8b60.0c25a99 240
Tryptophan 3.7a12.5c11b34 114
Valine 10.37±0.06a36.64±0.02b
Lysine 12.46±0.05a39.95±0.07b
Leucine 27.19±0.02a63.19±0.08b
Isoleucine 11.05±0.01a28.02±0.02b
Phenylalanine 13.52±0.02a31.03±0.01b
reonine 9.43±0.01a33.01±0.03b
Histidine 7.51±0.02a16.60±0.02b
Methionine 8.14±0.04a18.11±0.02b
Tryptophan 3.70±0.03a12.48±0.05b
Arginine 14.25±0.04a41.98±0.02b
Proline 19.82±0.02a50.28±0.01b
Aspartic acid 24.19±0.03a78.87±0.03b
Glutamic acid 63.75±0.05a152.92±0.08b
Serine 10.50±0.03a40.65±0.02b
Glycine 14.44±0.06a30.58±0.04b
Alanine 14.05±0.02a51.25±0.07b
Cysteine 16.63±0.04a41.89±0.04b
Tyrosine 6.65±0.02a24.56±0.02b
Total essential amino acids 103.37±0.01a279.03±0.01b
Total nonessential amino acids 184.28±0.02a512.98±0.03b
Ratio(essential/nonessential) 0.56±0.01a0.54±0.02c0.38b
Values are expressed as mean±SD(n=3). Means in the same row with distinct superscripts are significantly dierent(P≤0.05) as separated by Duncan multiple range
test. xAmino acid pattern of preschool children(2–5years)(FAO/WHO/UNU, 1985). SD: Standard deviation; PB: Banana pseudostem; FB: Banana ower
RAMITH RAMU, et al.: Nutritional Quality of Banana Flower and Pseudostem
Pharmacognosy Research, Volume 9, Supplement 1, December 2017 S81
Table 5: Enzymatic antioxidant potential and global antioxidant response of banana pseudostem and banana ower using dierent methods and distribution
of antioxidant activity in soluble and insoluble fractions after in vitro digestion (a); yield, total phenolic content and antioxidant activity of banana pseudostem
and banana ower sequential solvent extracts (b) and phenolic acids identication (c)
Methods PB FB
Enzymatic antioxidantswSuperoxide dismutase 14.56±0.70a19.08±1.66b
Catalase 3.68±0.54a7.86±1.01b
Ascorbate peroxidase 0.32±0.44a0.49±1.89b
glutathione reductase 0.76±2.61a1.53±0.47b
GARABTSvTot a l 54.07±0.25a70.15±0.55b
Soluble 37.58±1.88a45.62±1.38b
Insoluble 15.41±3.33a22.99±2.96b
Quencherx 17.86±0.69a24.05±1.98b
GARDPPHvTotal 0.78±0.60a1.37±2.09b
Soluble 0.49±1.60a0.99±1.23b
Insoluble 0.21±0.75a0.27±3.08b
Quencherx 0.33±0.38a0.42±1.08b
GARFRAPvTotal 3.47±1.65a6.53±1.34b
Soluble 2.02±1.03a4.99±1.77b
Insoluble 1.26±0.50a1.44±1.04b
Quencherx 1.59±0.54a2.78±0.82b
Extracts Yield (g/kg) TPCyABTSvDPPHvFRAPv
PB Methanol 25.45±0.46a98.98±0.58a11.98±1.87a0.22±0.17b1.23±1.01b
Ethanol 91.20±0.48c211.43±1.98c21.87±0.40b0.43±0.34c3.33±0.33c
Wat e r 89.09±0.58b122.34±0.41b10.64±1.50a0.20±0.67a1.06±0.46a
FB Methanol 61.37±0.55a121.59±0.58b18.80±1.31a0.25±1.24a1.58±1.79b
Ethanol 126.87±1.74c228.87±2.05c24.03±1.00b0.73±0.27b4.83±0.51c
Wat e r 101.84±0.54b105.78±0.48a18.08±0.98a0.24±2.50a1.44±2.00a
Phenolic acid PB (µg/mg) extract FB (µg/mg) extract
Methanol Ethanol Water Methanol Ethanol Water
Gallic acid 5.82 31.13 15.88 73.44 61.20 73.76
p‑hydroxybenzoic acid 11.48 62.68 32.78 61.65 94.97 19.43
Chlorogenic acid 6.09 11.87 8.61 14.08 13.76 14.42
Sinapic acid 14.91 37.06 3.19 2.02 3.22 9.81
Caeic acid 25.33 19.11 4.06 1.59 1.07
Vanillin 14.80 7.17 2.69 1.95 7.62 3.38
p‑coumaric acid 7.58 2.09 4.29 0.25 1.45
Epicatechin 3.07 0.72 5.37 0.52 0.75
Catechin 9.34 4.12 1.76 0.91 2.63
Quercetin 4.39 6.06 1.19 0.32 1.67
wUnits/min/mg of protein; vMmol equivalents of trolox/kg sample; xDirect procedure without extraction of PB and FB and expressed as in GAR (mmol equivalents of
trolox/kg sample); yMg equivalents of gallic acid/g. Values are expressed as mean±SD (n=3). Means in the same row with distinct superscripts are significantly dierent
(P≤0.05) as separated by Duncan multiple range test. SD: Standard deviation; PB: Banana pseudostem; FB: Banana ower; GAR: global antioxidant response; TPC:
Total phenolic content; ABTS: 2,2’‑azino‑bis, DPPH: 2,2‑diphenyl‑1‑picrylhydrazyl, FRAP: Ferric reducing antioxidant power
vanillin, p‑coumaric acid, quercetin, catechin, and epicatechin at dierent
concentrations. Ethanol extracts in both PB and FB were found to contain
high concentrations of phenolic acids in comparison to methanol and
aqueous extract. p‑hydroxybenzoic acid was the most predominant
phenolic acid recorded in PB and FB (62.7 μg/mg and 95 μg/mg),
followed by gallic acid(31.1 and 61.3μg/mg, respectively) with varying
concentrations. Caeic acid was predominant in the methanol extract
of PB (23.3 μg/mg), whereas gallic acid was predominant in that of
FB(73.4μg/mg). Although methanol and aqueous extract had phenolic
acids, the concentration was lesser than the ethanol extract[Table5c].
However, under physiological conditions, these results cannot be
reproduced by administering the extracted antioxidants directly.
Irrespective of the extraction method, some amount of the sample always
remains insoluble in one or the other solvent and hence Arda etal.[46]
developed a direct procedure(QUENCHER) to evaluate the TAC of
foods without an extraction step. Since, this method cannot dierentiate
between the physiologically active fraction and the insoluble one, a
combination of enzymatic digestion step for the soluble fraction and
the Quencher method for the insoluble fraction thus furnish an optimal
antioxidant potential of the given sample.
e results of the antioxidant activity using Quencher method[Table5a]
for PB and FB(ABTS: 17.8 and 24; DPPH: 0.33 and 0.42; FRAP: 1.59
and 2.78 mmol equivalents of the standard Trolox per kg of sample,
respectively) were in accordance with the results obtained by Arda
etal.[46] for dierent cereal products. e order of magnitude was same
as the GAR method for PB and FB samples despite a 2–3times reduction
in most parts of the results. Such a reduction could be attributed to the
absence of the enzymatic digestion step which could otherwise result in
dierent compounds obtained aer the enzymatic reactions. Overall,
the best results were obtained by the GAR method which exhibited
highest antioxidant activity with FB faring better than PB. In particular,
the insoluble fraction exhibited about 40%–50% of the total antioxidant
RAMITH RAMU, et al.: Nutritional Quality of Banana Flower and Pseudostem
S82 Pharmacognosy Research, Volume 9, Supplement 1, December 2017
activity and since this fraction is excluded during the extraction process,
this is the most recommended method for the measurement of TAC.
Although the antioxidant role of the insoluble fraction is questioned
since they are not extractable, they are expected to exert their eect by the
surface reaction phenomenon. Furthermore, some part of the insoluble
fraction may undergo digestion by the intestinal microora thus
releasing some substances which can also exert antioxidant properties
and considering these; it would be essential to measure the antioxidant
capacity of even the insoluble fraction of the digested food.[47]
Further, with respect to the antioxidant assays, the dierent anities of
the radicals to scavenge various antioxidant groups present in dierent
samples suggest the need to use more than a single assay to determine the
antioxidant potential of a particular sample. In this regard, in the present
study, the TAC as measured with two radical scavenging assays(ABTS
and DPPH) fared dierently for both the byproducts. In support of
these results, Roger etal.[48] demonstrated that the macromolecules are
seldom attacked by the hydrophobic radicals, which could be the reason
for the lower activity in DPPH as compared to the ABTS assay wherein
DPPH is a hydrophobic radical while ABTS is more of a hydrophilic
probe. Furthermore, DPPH being more selective in the reaction with
H‑donors, it could also be the reason for its lower TAC values in this
assay. Further, the FRAP activity which is based on the reduction of the
Fe+3–TPTZ complex in the ferrous form at low pH, exhibited 6.5 mmol
Trolox Eq./Kg for FB and for PB with a statistically signicant dierence
in the values(P>0.05). e results, however, in comparison with ABTS
were lower, but better than the DPPH assay.[49]
In addition, enzymatic(SOD, CAT, APX, and GR) antioxidant potential
has been evaluated for the FB and PB. As evident from Tab l e 5a, FB
showed maximum activity of SOD(19.1 U/min/mg protein) followed
by catalase(7.9 U/min/mg protein), GR(1.5 U/min/mg protein) and
APX(0.49 U/min/mg protein). On the other hand, PB also exhibited
enzymatic activities for SOD (14.6 U/min/mg protein) followed
by catalase (3.7 U/min/mg protein), GR (0.76 U/min/mg protein),
APX(0.32 U/min/mg protein) and found was to be lower in comparison
to FB. Higher SOD, APX, and GR enzymatic antioxidant activities in
PB and FB clearly indicates their greater ability to detoxify ROS such
as superoxide, hydroxyl, and peroxide radicals formed in human cell by
endogenous and exogenous factors which in turn could lead to geriatric
degenerative conditions, cancer and a wide range of other human
In summary, the present study manifests that both PB and FB possess
rich nutraceutical properties because of the presence of various bioactive
ingredients with numerous benets. It provides evidence that the two
banana byproducts are rich in proximate nutrient composition, minerals,
fatty acids, and antioxidants(both enzymatic and nonenzymatic) and
hence could be used in the human diet. e beneciary properties are
mainly derived from their minerals, carbohydrates, dietary bers and
proteins together with the low content of fat and calories. Furthermore,
as a rich source of phytochemicals, minerals and vitamins reside in
PB and FB they can be further evaluated for use as a key ingredient
forvaluable drugs. To add to these, the high total dietary fiber content
and a balanced ratio between insoluble dietary fiber and soluble dietary
fiber in both PB and FB are attractive targets for the food industry. ese
could be used in the development of a nutritional supplement because of
their health‑related properties of dietary fiber and associated bioactive
In addition to the strong basis provided by the nutritional aspects
of PB and FB, their potential as antioxidants are also conrmed by
a series of studies which included dierent methods of extraction as
well as dierent assays to determine their antioxidant potential. It is
demonstrated that the GAR method exhibited antioxidant activity higher
than that reported with traditional procedures, which asserts the role of
both insoluble as well as soluble fractions of the digested food to possess
antioxidant properties. To summarize on the whole, this paper reinforces
the concept that PB and FB are potent sources of several biologically
active ingredients and also possess rich antioxidant property.
Financial support and sponsorship
Conicts of interest
ere are no conicts of interest.
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... The carbohydrate, protein, and starch content in the Banana pseudo stem is approximately (46.58%), (7.34%), and (21.06%) respectively. The total dietary fiber content of pseudo stem is (61.14%) of which the soluble dietary fiber and the insoluble dietary fiber (IDF) are approximately (02.04%), and (59.10%) respectively [4]. High dietary fiber diets are effective at producing early satiety signals by lengthening the time food remains in the stomach, lowering the risk of stomach ulcers. ...
... Thus, a diet high in fiber promotes digestion, as well as waste removal and preventing constipation. This suggests that banana by-products may eventually substitute oats and sorghum as a fiber-rich food source [4]. ...
... Higher levels of flavonoids and saponins were found in the pseudo stems of two Musa spp. Bananas, Baxijiao (saponins: 0.11 g/100 g), and Paradisiacal (saponins: 0.12 g/100 g), and given these advantages, the promise of Banana pseudo stems for their healthpromoting qualities is affirmed [4]. In a study, it was observed that ascorbic acid content was high in Banana pseudo stem (8.81mg/100g) compared to other vitamins, such as vitamin E, riboflavin, thiamine, niacin, beta carotene, pyridoxine, and pantothenic acid [5]. ...
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Musaceae is a perennial tree-like plant that is cultivated all over the world in various tropical and subtropical regions. The fruit, peel, leaves, pseudo-stem, stalk, and inflorescence (flower) of this plant, among other parts, are utilized in traditional medicine to treat several health problems. The banana stem juice has potential biological effects, including anti-diabetic, anti-inflammatory, anti-microbial, anti-helminthic, wound-healing, anti-cancer, anti-oxidant, and anti-urolithic activities. Consequently, it can be said that banana stem juice contains adequate levels of healthy secondary metabolites that are essential for maintaining good health. To find novel approaches for treating and preventing a wide range of medical diseases, more studies into the medicinal potential of banana stem juice can be done. Hence, this review emphasizes Musaceae stem juice's medicinal and nutritional benefits as review.
... The ratio of essential amino acids to nonessential amino acids required by the human body in banana inflorescences is 0.54 [36], which is greater than the amount advised for adults by the WHO. Bhaskar et al. [37] found that the protein content of banana inflorescences was 12.50%, which include high concentrations of aspartic acid, glutamic acid, alanine, arginine, leucine, proline, and serine. ...
... Sheng et al. [39] found that unsaturated fatty acids (UFAs) such as linoleic acid, oleic acid, and alpha-linolenic acid accounted for more than 60% of the total fatty acids in banana inflorescences. Linoleic acid has the effect of lowering cholesterol in the body and preventing atherosclerosis [36]. This shows that banana inflorescences can be an important source of healthful UFAs, which may lower the chance of cardiovascular disease [40]. ...
... Among them, potassium is the most abundant macronutrient, ranging between 33.52 and 55.2 mg/kg [41]. The next most abundant macronutrients are magnesium and calcium [33,36,42], but the inflorescences also have significant concentrations of iron, copper and zinc in terms of its micro elements, while iron is an essential element in human physiology and is one of the main raw materials for hemoglobin [19]. Therefore, banana inflorescences are an important source of minerals for human beings [41]. ...
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Bananas are among the world’s main economic crops and one of the world’s most-selling fresh fruits. However, a great deal of waste and by-products is produced during banana harvesting and consumption, including stems, leaves, inflorescences, and peels. Some of them have the potential to be used to develop new foods. Furthermore, studies have found that banana by-products contain many bioactive substances that have antibacterial, anti-inflammatory, and antioxidant properties and other functions. At present, research on banana by-products has mainly focused on various utilizations of banana stems and leaves, as well as the extraction of active ingredients from banana peels and inflorescences to develop high-value functional products. Based on the current research on the utilization of banana by-products, this paper summarized the composition information, functions, and comprehensive utilization of banana by-products. Moreover, the problems and future development in the utilization of by-products are reviewed. This review is of great value in expanding the potential applications of banana stems, leaves, inflorescences, and peels, which will not only help to reduce waste of agricultural by-product resources and ecological pollution but will also be useful for the development of essential products as alternative sources of healthy food in the future.
... In 5 mm NMR tubes, the isolated compound was prepared using deuterated solvent and tetramethylsilane (TMS) as an internal standard. Chemical changes corresponding to the TMS signal were detected in CDCl 3 and expressed in parts per million (d) (Ramu et al., 2017). ...
... The eluents were measured at 254 nm with the flow rate of 0.2 mL/min. The mass spectrum (MS) was acquired in ESI mode using a nitrogen nebulizer gas flow of 1.5 l/min, a CDL temperature of 250 C, a block temperature of 200 C, a detector voltage of 1.6 kV, and a CDL temperature of 250 C (Ramu et al., 2017). ...
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2022): Inhibitory effect of gallic acid from Thunbergiamysorensis against α-glucosidase, α-amylase, aldose reductase and their interaction: Inhibition kinetics and molecular simulations, Journal of Biomolecular Structure and Dynamics, ABSTRACT In this exploration, we assessed the antihyperglycaemic properties of methanol extract of flowers of Thunbergia mysorensis (MeT) against a-glucosidase, a-amylase and aldose reductase enzymes for the effective management of postprandial hyperglycemia. Hyperglycemia occurs when the body lacks enough insulin or is unable to correctly utilize it. MeT inhibited both the carbohydrate digestive enzymes (a-glucosidase and a-amylase) and aldose reductase, which are vital for the therapeutic control of postprandial hyperglycaemia. MeT was also found to have significant antioxidant activity. Using several spectroscopic approaches, the primary active component found in MeT was identified as gallic acid. With low Ki values, gallic acid significantly inhibited a-glucosidase (30.86 mg/mL) and a-amylase (6.50 mg/mL). Also, MeT and gallic acid both inhibited aldose reductase effectively, corresponding to an IC 50 value of 3.31 and 3.05 mg/mL. Our findings imply that the presence of polyphenol compounds (identified via HPLC analysis) is more likely to be responsible for the antihyperglycaemic role exhibited by MeT via the inhibition of a-glucosidase and the polyol pathway. Further, gallic acid interacted with the key residues of the active sites of a-glucosidase (À6.4 kcal/mol), a-amylase (À5.8 kcal/mol) and aldose reductase (À5.8 kcal/mol) as observed in the protein-ligand docking. It was also predicted that gallic acid was stable inside the binding pockets of the target enzymes during molecular dynamics simulation. Overall, gallic acid derived from MeT via bioassay-guided isolation emerges as a natural antidiabetic drug and can be taken into in vivo and clinical studies shortly. ARTICLE HISTORY
... Bract, a part of banana inflorescence, also known as flower or blossom, is typically treated as agricultural waste, although it is widely used in different regions for culinary purposes and in folk medicine [3]. Banana inflorescence has gained scientific relevance due to its variety of bioactive compounds and its associated beneficial health properties, including antioxidant, anti-diabetic, anti-microbial, anti-inflammatory and cardiovascularprotective activities [4][5][6][7][8][9][10]. ...
... Nanjangud rasa bale) and pseudostem. Phytochemical constituents as phenols showed values of 2.01 and 1.88 mg g −1 and flavonoids exhibited values of 0.83 and 0.78 mg g −1 for banana inflorescence and pseudostem, respectively [5]. In another study, banana inflorescence (Musa sp. ...
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This study investigated the effect of enzymatic hydrolysis of banana bracts from different varieties (Maçã, Nanica and Prata) using pectinase, protease and cellulase (singly or in combinations) on their antioxidant properties. The results showed that the antioxidant properties and total phenolic compounds (TPC) of extracts increased after the enzymatic treatment with a clear synergistic effect between the different enzymes. The ternary mixture of pectinase, protease and cellulase resulted in increases of 458% and 678% in TPC content for extracts obtained from Maçã and Nanica varieties and up to 65% in antioxidant properties of those produced from Prata variety compared to the non-hydrolyzed samples. In general, the extracts obtained from the Prata variety showed the highest levels of TPC, as well as antioxidant activity, as follows: 14.70 mg GAE g−1 for TPC, 82.57 µmol TE g−1 for ABTS, 22.26 µmol TE g−1 for DPPH and 47.09 µmol TE g−1 for FRAP. Phenolic compounds identified by HPLC in extracts included ρ-coumaric, ferulic, sinapic and vanillic acids and the flavonoid rutin. This study reported for the first time the enzymatic treatment applied to banana bracts as a promising method to release antioxidant compounds, offering a new opportunity to explore these residues as a source of molecules with high added value through an environmentally friendly and safe process.
... Banana inflorescences have been reported to exhibit health benefits, being a rich source of high-quality proteins, dietary fibers, vitamins, minerals, and functional phytochemicals ( Bhaskar et al., 2011 ;Thaweesang, 2019 ;Lau et al., 2020 ;Ramírez-Bolaños et al., 2021 ). Moreover, the ratio of essential to non-essential amino acids of banana inflorescences is 0.54, which is higher than the WHO recommendation ( Ramu et al., 2017a ). majority of these texture-modified foods and thickened liquids lack nutritional and sensory qualities ( Keller et al., 2012 ). ...
... Bhaskar et al.(2012) reported that the soluble and insoluble dietary fiber (%) of the banana pseudostem was 1.4 and 27.4 and the ash (%) content of banana pseudostem was 0.3. Ramu et al.(2017) reported that the phenols and flavonoids (mg/100g) content of the banana pseudostem was 188.64 and 78.60. The tannins (mg/100g) levels of pseudostem were 7.86. ...
Minimal processing techniques should provide the market with fresh- like appearance. Fresh-cut vegetables are consumed widely and it is a healthy, nutritional and convenient option. The present study aimed to standardize and develop the minimally processed precut pseudostem. Two banana varieties were selected viz., ottuvazhai (syn Mupaddai) (V1), vayalvazhai (V2) for developing minimally processed product.To prevent browning, different pretreatments such as lemon juice, turmeric powder, curd, salt, water, citric acid, and vinegar were used at 1 to 5 % concentration. Two different packaging materials, viz., aluminum foil (P1) and low-density polypropylene (P2) were used.The chemical composition of banana pseudostem was analyzed. The moisture, browning index, color value and microbial study of the precut pseudostem were analyzed using standard procedures. The quality of minimally processed precut pseudostem was evaluated from the initial day to 9 days at ambient and refrigerated storage conditions. Among the pretreatments, citric acid pretreated precut pseudostem had an effective role against browning reaction.During storage conditions, a minimum changes were observed in Mupaddai variety (V1) precut pseudostem sample. At refrigerated condition, the minimally processed product (V1) from aluminum foil was good upto 9 days of storage condition.
... Results were obtained from the previous work of the authors, where the HR-LCMS analysis of the ethanol extract of M. champaka leaves was performed (Pushpa et al., 2022). Phytochemicals obtained from both positive and negative electron spray methods were taken into consideration (Supplementary Table S1a and S1b) (Ramu et al., 2017). However, it cannot be excluded that there may be margins of error in the methodology described for their phytochemical profiling of M. champaka leaves. ...
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Phytochemical-based drug discovery against the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been the focus of the current scenario. In this context, we aimed to perform the phytochemical profiling of Magnolia champaka, an evergreen tree from the Magnoliaceae family, in order to perform a virtual screening of its phytoconstituents against different biological targets of SARS-CoV-2. The phytochemicals identified from the ethanol extract of M. champaka leaves using liquid chromatography-mass spectroscopy (LC-MS) technique were screened against SARS-CoV-2 spike glycoprotein (PDB ID: 6M0J), main protease/Mpro (PDB ID: 6LU7), and papain-like protease/PLpro (PDB ID: 7CMD) through computational tools. The experimentation design included molecular docking simulation, molecular dynamics simulation, and binding free energy calculations. Through molecular docking simulation, we identified poncirin as a common potential inhibitor of all the above-mentioned target proteins. In addition, molecular dynamics simulations, binding free energy calculations, and PCA analysis also supported the outcomes of the virtual screening. By the virtue of all the in silico results obtained, poncirin could be taken for in vitro and in vivo studies in near future. Communicated by Ramaswamy H. Sarma
... The fruit part of banana is widely used and regularly consumed due to its nutritional value and health benefits, while banana blossoms are usually unfamiliar and commonly underutilized in most parts of the world [2]. Banana blossoms, together with banana pseudo stems, are regarded as wastes [3]. Studies show that banana blossoms have great potential for their high nutritional value and medicinal benefits [4]. ...
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This study evaluated the proximate composition, and content of phenolic and flavonoid compounds as well as the potential antioxidant property of saba (Musa acuminata x balbisiana Colla) banana blossoms. The banana blossom was dried at 60 ℃ for 12 hours. The sample was then extracted using 80% ethanol. The ethanolic extract of the sample was subjected to antioxidant and various characterization, including the following: i) nutritional values of the banana blossom powder, which was analyzed according to the Association of Official Analytical Chemists (AOAC), ii) total phenol and total flavonoid content and iii) antioxidant test using, 2-diphenyl-1picrylhydrazyl (DPPH) free radical scavenging activity. The results revealed that the sample contains a high amount of ash, fiber, protein, and carbohydrate and a low amount of fat. The Total Phenolic Content and Flavonoid Concentration of extract revealed an abundant reservoir of compounds, the concentration 500 mcg/mL, exhibited the highest radical scavenging activity of banana blossom ethanolic extract, suggesting a dose-dependent activity. Further investigation using other in vitro and in vivo antioxidant assay is recommended to confirm the potential use of saba (Musa acuminata x balbisiana Colla) banana blossom as an antioxidant.
... used in functional food as substitution of flour in cookies(Saguilan et al., 2007). Total carbohydrates (%) of the seven varieties of banana pseudostem ranged from 20.5 to 38.8 %. The total carbohydrate was high in the variety Karpooravalli with 38.8 percent. The lowest carbohydrates content was observed in the variety Red Banana with 20.5 percent.Ramu et al. (2017) reported that the total carbohydrate in the banana pseudostem of Musa sp. cv. Nanjangud rasa bale was 46.58 %. The difference may be due to different drying condition or part of pseudostem used. ...
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Banana (Musa sp.) belongs to the family Musaceae. Banana pseudostem is a by-product of banana cultivation. It has many potential for incorporating as a source of nutrients in the food products. For the effective utilization of banana pseudostem and to reduce the disposal problem, it can be used in food products for improving the nutritional value. The aim of the study is to analyze the nutritional and biochemical properties of pseudostem powder of seven different varieties namely Poovan, Nendran, Monthan, Karpooravalli, Ney Poovan, Grand Naine and Red Banana. The banana pseudostem powder recorded moisture content (7.2%), pH (7.1), total soluble solids (1.5 ˚Brix), ash (4.1%), titratable acidity (0.05%), protein (3.3%), total carbohydrates (29.3 %) and vitamin C content (6.6 mg/ 100g). The biochemical analysis showed that the proximate contents i.e., total soluble solids, titratable acidity, total carbohydrates and vitamin C content were highest in the variety Karpooravalli whereas ash content and protein were highest in the variety Ney Poovan and Monthan respectively. These findings will be useful in the further studies in utilization of banana pseudostem in food industries for making products from banana.
In global exports, the commodity banana generates billions of dollars annually, a market which is accompanied by the discard of extraordinary amounts of valuable agricultural material from banana trees, mainly pseudostems, leaves, and hearts (banana inflorescence). The recovery of high-added value natural compounds from agri-food by-products is an emerging topic with plentiful research focused on extraction techniques for the biomass valorization, highlighting the circular economy concept. In this context, the present review discusses the current uses and processing of the most abundant banana tree agricultural wastes (pseudostems, leaves and inflorescence). Multiple extractives and applications were investigated considering the banana type, physical-chemical characteristics, and co-product treatments. Strategic areas for human development, such as food, pharmaceutical, civil construction, energy, and fibers, are addressed in association with the use of banana biomasses, and correlated with the United Nations Sustainable Development Goals. Finally, the social and environmental areas mostly impacted by the green chemistry and biorefinery were also highlighted for the banana industrial chain.
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Heliotropium bacciferum is paramount in medicinal perspective and belongs to Boraginaceae family. The crude and numerous fractions of leaves, stem, and roots of the plant were investigated for phytochemical analysis and DPPH radical scavenging activity. Phytochemical analysis of crude and fractions of the plant revealed the presence of alkaloids, saponins, tannins, steroids, terpenoids, flavonoids, glycosides, and phenols. The antioxidant (free radical scavenging) activity of various extracts of the Heliotropium bacciferum was resolute against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical with the avail of UV spectrophotometer at 517 nm. The stock solution (1000mg/mL) and then several dilutions (50, 100, 150, 200, and 250mg/mL) of the crude and fractions were prepared. Ascorbic acid was used as a standard.The plant leaves (52.59 ± 0.84 to 90.74 ± 1.00), stem (50.19 ± 0.92 to 89.42 ± 1.10), and roots extracts (49.19 ± 0.52 to 90.01 ± 1.02) divulged magnificent antioxidant activities. For the ascertainment of the fatty acid constituents a gas chromatograph hyphenated tomass spectrometer was used.The essential fatty acids for growthmaintenance such as linoleic acid (65.70%), eicosadienoic acid (15.12%), oleic acid (8.72%), and palmitic acid (8.14%) were found in high percentage. The infrared spectra of all extracts of the plant were recorded by IR Prestige-21 FTIR model.
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Postprandial hyperglycaemia is characterized as the earliest symptom of diabetes and its management attenuates several of the associated secondary complications. In this context, we investigated the role of ethanol extract of banana flower (EF) for its antihyperglycaemic effects. The EF showed a strong inhibition towards α-glucosidase and pancreatic amylase which play a vital role in clinical management of postprandial hyperglycaemia. The major active compounds present in EF were identified as Umbelliferone (C1) and Lupeol (C2) using various spectroscopic methods. C1 (IC50: 7.08 ± 0.17 μg/ml) and C2 (IC50: 7.18 ± 0.14 μg/ml) were found to inhibit α-glucosidase in a non-competitive mode of inhibition, with low Ki values. Further, in vitro glycation assays showed that EF and its compounds prevented each stage of protein glycation and formation of its intermediary compounds. EF, C1 and C2 also exhibited a potent inhibition on aldose reductase with IC50 values of 2.25 ± 0.29, 1.32 ± 0.22 & 1.53 ± 0.29 μg/ml respectively. Our results suggest that, the observed potential of EF in antihyperglycaemic activity via inhibition of α-glucosidase and in antidiabetogenic effect by inhibition of polyol pathway and protein glycation is more likely to be attributed to the presence of C1 and C2.
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Background Postprandial hyperglycemia in diabetes could be ameliorated by inhibiting intestinal α-glucosidases, responsible for starch hydrolysis and its absorption. Different parts of banana have been in use in conventional medicinal formulation since ancient times. Its role as antihyperglycemic agent has also been studied. This study was aimed at explaining the mechanism of hypoglycemic effect by ethanol extract of banana pseudostem (EE). Besides, studies on the active components involved in the effect have also been attempted.ResultsEE significantly inhibited mammalian intestinal α-glucosidases and yeast α-glucosidase (IC50: 8.11 ± 0.10 µg mL−1). The kinetic studies showed that EE inhibited sucrase, maltase and pNPG hydrolysis by mixed-type inhibition. Further, in vivo studies identified that the oral administration (100 to 200 mg kg−1 body wt.) of EE significantly suppressed the maltose/glucose-induced postprandial plasma glucose elevation and wielded an antihyperglycemic effect in normal and alloxan-induced diabetic rats. GC-MS analysis of EE revealed high levels of β-Sitosterol (29.62%), Stigmasterol (21.91%), Campesterol (10.85%) and other compounds.Conclusion These findings suggest that EE might exert an antidiabetic effect by inhibition of α-glucosidases from the intestine, in turn suppressing the carbohydrate absorption into the blood streams. Hence the results extend a foundation to the future prospects of the food-derived enzyme inhibitors in treatment of diabetes.
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This study was designed to determine the primary and secondary metabolites present from the leaves of Phyllanthus wightianus using various analytical techniques. Furthermore the antioxidant and anti-inflammatory activities of ethanolic extract of the leaves of P. wightianus were investigated using standard models. The results show that the leaves exhibit good antioxidant activity and protective to HRBC (Human Red Blood Cell) membrane due to the presence of some valuable phytochemicals present in the leaves.
Banana fibers obtained from the stem of banana plant (Musa sapientum) have been characterised for their diameter variability and their mechanical properties, with a stress on fracture morphology. The nature of representative stress strain curves and fracture at different strain rates have been analysed through SEM.
This study aimed to investigate the effects of solvent composition of different radicals (ABTS, DPPH) on measured total antioxidant capacity (TAC) of foods determined by the QUENCHER procedure. The working solutions of ABTS radical were prepared in the mixture of water-ethanol with different volume ratios (0:100, 25:75, 50:50, 75:25, 100:0). The solvent composition had a significant effect on the measured antioxidant capacity of various food matrices including cereals, fruits and vegetables, pulses and nuts (p < 0.05). The use of ethanol alone gave the lowest values during measurement while introducing water to ethanol significantly improved the levels of antioxidant capacity. These results suggested that the mixture of-water-ethanol (50:50, v/v) may be the most appropriate solution for standardizing the TAC database of foods tested by the QUENCHER procedure. The need of water is due to its ability to open the structure enabling better access of radicals to functional ends of the food matrices.
Correlations between total phenolic and mineral contents with antioxidant activities of pulps and peels from eight banana (Musa sp.) cultivars were studied. The total phenolic contents were determined using Folin–Ciocalteu colorimetric method, and antioxidant activities were measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant potential (FRAP) assay. The highest total phenolic content (76.37±1.79mgGAE/gd.w.) was obtained from the freeze-dried extract of fresh pulps of Raja cultivar. The maximum activity of DPPH (19.39±0.15mgTE/gd.w.) was recorded for the chloroform extract of dried peels of Mas cultivar. Meanwhile, the highest activity of FRAP was shown by most of the chloroform extracts of dried pulps, dominated by Awak cultivar (22.57±0.13mgTE/gd.w.). With few exceptions, peel extracts exhibited higher total phenolic content and stronger antioxidant activities than that of the pulps. Very weak correlation between total phenolic content and FRAP activity was observed, yet it is higher (r2=0.1614, p
The three maturity stages found in the pericarp and seeds of bitter melon (Momordica charantia) were investigated for their variations in proximate composition, amino acid profiles, and mineral contents. Moisture, starch, and total dietary fibre contents of pericarp, from all maturity stages (immature, mature and ripe), were significantly higher (P value <0.05) than those of ripe and mature seeds, while lipid and protein contents of seeds were statistically higher (P value <0.05) than those of pericarp. Maturity did not change the lipid content of the pericarp, while maturity progression decreased the protein content of bitter melon pericarp. A significant increase in the protein (30.4%) and lipid (37.6%) contents was observed in bitter melon seeds as the maturity progressed. Ripe seeds that have more than 30% protein could be a good protein source for functional ingredients in a food system. Bitter melon can be considered a good source of these nutrients. CD