Female pattern hair loss: A pilot study investigating combination therapy with low-dose oral minoxidil and spironolactone

Abstract

Background:
Minoxidil and spironolactone are oral antihypertensives known to stimulate hair growth.

Objective:
To report on a case series of women with pattern hair loss (PHL) treated with once daily minoxidil 0.25 mg and spironolactone 25 mg.

Methods:
Women newly diagnosed with a Sinclair stage 2-5 PHL were scored for hair shedding and hair density before and after 12 months of treatment with oral minoxidil 0.25 mg and spironolactone 25 mg.

Results:
A total of 100 women were included in this observational pilot study. Mean age was 48.44 years (range 18-80). Mean hair loss severity at baseline was Sinclair 2.79 (range 2-5). Mean hair shedding score at baseline was 4.82. Mean duration of diagnosis was 6.5 years (range 0.5-30). Mean reduction in hair loss severity score was 0.85 at 6 months and 1.3 at 12 months. Mean reduction in hair shedding score was 2.3 at 6 months and 2.6 at 12 months. Mean change in blood pressure was -4.52 mmHg systolic and -6.48 mmHg diastolic. Side effects were seen in eight women but were generally mild. No patients developed hyperkalemia or any other blood test abnormality. Six of these women continued treatment, and two women who developed urticaria discontinued treatment.

Limitations:
Prospective, uncontrolled, open-label observational study.

Discussion:
Once daily capsules containing minoxidil 0.25 mg and spironolactone 25 mg appear to be safe and effective in the treatment of FPHL. Placebo-controlled studies to investigate this further are warranted.

Full text

Pharmacology and therapeutics
Female pattern hair loss: a pilot study investigating
combination therapy with low-dose oral minoxidil and
spironolactone
Rodney D. Sinclair
1,2
,MBBS, MD, FACD
1
Epworth Hospital, East Melbourne, Vic.,
Australia, and
2
Sinclair Dermatology Clinical
Trial Centre, East Melbourne, Vic., Australia
Correspondence
Rodney D. Sinclair, MBBS,MD,FACD
Epworth Hospital
East Melbourne, Vic.
Australia
E-mail: rodney.sinclair@
epworthdermatology.com.au
Funding: None.
Conflicts of interest: Rodney Sinclair holds
Innovation Patent 2011100917 entitled
Treatment of male and female androgenetic
alopecia with oral minoxidil either alone or
in combination with antiandrogens on 18
August 2011.
Abstract
Background Minoxidil and spironolactone are oral antihypertensives known to stimulate
hair growth.
Objective To report on a case series of women with pattern hair loss (PHL) treated with
once daily minoxidil 0.25 mg and spironolactone 25 mg.
Methods Women newly diagnosed with a Sinclair stage 2–5 PHL were scored for hair
shedding and hair density before and after 12 months of treatment with oral minoxidil
0.25 mg and spironolactone 25 mg.
Results A total of 100 women were included in this observational pilot study. Mean age was
48.44 years (range 18–80). Mean hair loss severity at baseline was Sinclair 2.79 (range 2–
5). Mean hair shedding score at baseline was 4.82. Mean duration of diagnosis was
6.5 years (range 0.5–30). Mean reduction in hair loss severity score was 0.85 at 6 months
and 1.3 at 12 months. Mean reduction in hair shedding score was 2.3 at 6 months and 2.6
at 12 months. Mean change in blood pressure was 4.52 mmHg systolic and 6.48 mmHg
diastolic. Side effects were seen in eight women but were generally mild. No patients
developed hyperkalemia or any other blood test abnormality. Six of these women continued
treatment, and two women who developed urticaria discontinued treatment.
Limitations Prospective, uncontrolled, open-label observational study.
Discussion Once daily capsules containing minoxidil 0.25 mg and spironolactone 25 mg
appear to be safe and effective in the treatment of FPHL. Placebo-controlled studies to
investigate this further are warranted.
Capsule summary
•Oral minoxidil is an antihypertensive that causes hypertri-
chosis.
•Spironolactone is a diuretic with antiandrogen properties used
in the treatment of female pattern hair loss.
•One hundred women with female pattern hair loss were trea-
ted off-label with extemporaneously formulated oral capsules
containing minoxidil 0.25 mg and spironolactone 25 mg.
•Mean reduction in hair loss severity score was 0.85 at
6 months and 1.3 at 12 months. Mean reduction in hair shed-
ding score was 2.3 at 6 months and 2.6 at 12 months.
•Side effects were seen in eight patients and included postural
hypotension, hypertrichosis, and urticaria.
Introduction
Female pattern hair loss (FPHL) is one of the most common
causes of hair loss encountered in clinical practice.
1
FPHL is a
complex polygenic disorder
2–5
characterized clinically by diffuse
hair thinning over the midfrontal scalp
6
and increased hair shed-
ding.
7
Histologically, the hallmark is site-specific hair follicle
miniaturization.
8
Site specificity may result from epigenetic mod-
ification of the androgen receptor gene.
9
The proportion of
miniaturized follicles increases with the severity of hair loss.
10
Age-related, so-called senescent alopecia also shows hair folli-
cle miniaturization and is indistinguishable from FPHL.
11
FPHL
adversely impacts on quality of life.
12
FPHL is progressive, and
the risk, prevalence, and severity of FPHL increase with age.
13
In a population study of over 700 women, FPHL, defined as ≥
Sinclair stage 2, was found in 12% of women aged 20–29 and
57% of women aged ≥80. Severe hair loss, defined as Sinclair
stages 3, 4, and 5, increased from 4% among women aged 20–
29 years to 30% among women aged ≥80 years. In addition,
some women present with increased hair shedding but no clini-
cal evidence of FPHL. Approximately 60% of these women will
have histological evidence of androgenetic alopecia on scalp
biopsy with a terminal to vellus hair ration ≤4:1.
14
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104

Hair follicle miniaturization is potentially reversible initially but
eventually becomes irreversible.
15–17
One hypothesis to explain
irreversible hair follicle miniaturization is the observed replace-
ment of the proximal arrector pili muscle by adipose tissue dis-
rupting the stem cell niche at the hair follicle bulge.
17,18
Fatty
degeneration of the arrector muscle is not seen in alopecia
areata where hair follicle miniaturization is potentially reversi-
ble.
18
Treatment is likely to be most successful in women with
early female pattern hair loss.
19
While scalp biopsy may be required to identify histological
features of androgenetic alopecia in women with early FPHL
and differentiate this condition from chronic telogen effluvium,
20
dermoscopy is a valuable alternative and shows a reduction in
the number of secondary hair fibers emerging from each pore
over the affected region of the scalp.
17,21
A number of agents have also been used in the treatment of
female pattern hair loss including the androgen receptor antago-
nists spironolactone, cyproterone acetate,
19
and flutamide
22
as
well as the 5 areductase antagonist finasteride
23
and dutas-
teride. These agents can be used either alone or in combination
with topical minoxidil.
24
Minoxidil is a piperidinopyrimidine derivative and a potent
vasodilator that is effective orally for severe hypertension. When
applied topically, minoxidil has been shown to arrest hair loss or
to induce mild to moderate hair regrowth in approximately 60% of
women with FPHL.
25
A clinical trial comparing 5% and 2% formu-
lations of minoxidil found a mean increase in nonvellus hair
counts after 48 weeks of 18% and 14%, respectively.
26
Topical
minoxidil was approved by the FDA in 1992 for the treatment of
female pattern hair loss. It appears to be a safe therapy with side
effects only of local irritation and hypertrichosis of the temples,
and there is a low incidence of contact dermatitis.
27
If treatment
is stopped, clinical regression occurs within 6 months, to the
state of baldness that would have existed if treatment had not
been applied.
28
For patients to maintain any beneficial effect,
applications must continue indefinitely.
Spironolactone is an aldosterone antagonist and has been used
as a potassium-sparing diuretic for over 50 years. It is structurally
a steroid, with basic steroid nuclei with four rings. Its primary
metabolite, canrenone, is the active antagonist of aldosterone and
contributes to the diuretic action. The ingested drug is absorbed
rapidly and metabolized by the liver to canrenone and potassium
canrenoate. The drug is available in 25 and 100 mg tablets. No
dermatologic indications for spironolactone have been approved
by the FDA; however, it is widely used off-label in the treatment of
FPHL
29
and has been shown to arrest progression in over 90% of
women. In addition, approximately, 30% of women demonstrate
improved standardized scalp photographic assessment.
19
Hair transplantation surgery is a highly effective treatment for
male pattern hair loss. For women surgical options are limited.
Most women with FPHL also have reduced hair density over the
occipital scalp, reducing the yield from hair transplant surgery.
We report the results of a prospective, uncontrolled observa-
tional study of the safety and usefulness of a single, once daily
low-dose oral minoxidil in combination with spironolactone in
the treatment of FPHL.
Materials and methods
Women with a Sinclair stage 2–5 female pattern hair loss were
offered treatment with a single once daily capsule containing
minoxidil 0.25 mg together with spironolactone 25 mg. For
1 = 10
2 = 50
3 = 100
4 = 200
5 = 400
6 = 750
Figure 1 Sinclair hair shedding scale.
Patients were asked how much hair they
shed in a single day. As hair shedding is
usually worse after washing, that score was
documented
ª2017 The International Society of Dermatology International Journal of Dermatology 2018, 57, 104–109
Sinclair Oral minoxidil and spironolactone for FPHL Pharmacology and therapeutics 105

women with a baseline blood pressure ≤90/60 or a history of
postural hypertension or fainting, 50 mg of sodium chloride was
added to the capsule. Hair shedding was scored using a six-
point visual analogue scale (Fig. 1). Hair density was scored
using the 5 stage Sinclair scale (Fig. 2). Women were reviewed
at 3 monthly intervals. Blood pressure was recorded at each
visit, and patients were specifically questioned about the
presence of unwanted facial or body hair at each follow-up visit
and any other side effects.
Full blood count, renal function, electrolytes, and liver function
testing were performed at baseline and at 3 monthly intervals.
Results
One hundred women with newly diagnosed Sinclair stage 2–5
female pattern hair loss were treated with a once daily capsule
containing minoxidil 0.25 mg and spironolactone 25 mg and
followed prospectively for 12 months.
The mean age was 48.44 years (range 18–80). Mean hair
loss severity at baseline was Sinclair 2.79 (range 2–5). The
mean hair shedding score at baseline was 4.82 (range 1–6).
Mean duration of diagnosis was 6.5 years (range 0.5–30).
Side effects were seen in eight women but were generally
mild. Side effects included urticaria (2), postural hypotension
(2), and facial hypertrichosis (4). No patients developed hyper-
kalemia or any other blood test abnormality. Six of these
women continued treatment, and two women who developed
urticaria discontinued treatment.
Baseline mean systolic blood pressure was 122.92 mmHg.
Baseline mean diastolic pressure was 79.17 mmHg. Follow-up
blood pressure after 3 months was 118.40 systolic and 72.69
diastolic. Mean change in systolic blood pressure was
4.52 mmHg. Mean change in diastolic blood pressure was
6.48. Two patients developed symptoms of postural hypoten-
sion necessitating introduction of 50 mg daily of sodium chloride.
Four patients reported hypertrichosis. This was managed by
a combination of plucking (1) or waxing (3).
A temporary increase in hair shedding 3–6 weeks following ini-
tiation of treatment was anticipated. Twenty-two patients
reported this shedding as being of significant concern. All
patients had been prewarned about the possibility of a temporary
increase in hair shedding on initiation of therapy and advised to
continue treatment. No women discontinued the treatment as a
result of increased hair shedding following commencement of
therapy. For 16 women, this shedding ceased within 4 weeks,
while for four women it persisted for more than 6 weeks and for
two women, it persisted for more than 12 weeks.
Two patients ceased the medication because of urticaria that
was presumed to be related to the spironolactone. The urticaria
settled within 7 days of cessation and did not recur when the
minoxidil was recommenced as monotherapy.
Mean hair loss severity at baseline was Sinclair 2.79 (range
2–5). Mean hair shedding score at baseline was 4.82 (range 1–6).
1
2
4
3
5
Figure 2 Sinclair hair loss severity scale for female pattern hair
loss
International Journal of Dermatology 2018, 57, 104–109 ª2017 The International Society of Dermatology
Pharmacology and therapeutics Oral minoxidil and spironolactone for FPHL Sinclair
106

Patient 1
Patient2
Patient 3
Patient 4
Patient 5
Figure 3 Before and after 12-month therapy
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Sinclair Oral minoxidil and spironolactone for FPHL Pharmacology and therapeutics 107

Mean reduction in hair loss severity score was 0.1 at 3 months,
0.85 at 6 months, 1.1 at 9 months, and 1.3 at 12 months (Fig. 3).
Mean reduction in hair shedding score was 1.1 at 3 months, 2.3
at 6 months, 2.7 at 9 months, and 2.6 at 12 months.
Discussion
Oral minoxidil was approved by the FDA for the treatment of
hypertension in 1979. It was first noticed to improve hair loss in
male androgenetic alopecia in 1980.
30
Topical minoxidil
received FDA approval for male androgenetic alopecia in 1988
and for female pattern hair loss in 1992.
Oral minoxidil is not often used in the treatment of AGA, lar-
gely because of the side-effect profile seen at standard doses.
Our women’s hair loss clinic was established in 1995 and
currently treats over 750 women with FPHL. The mainstay of
therapy was an oral antiandrogen such as cyproterone acetate
or spironolactone used either alone
19
or together with topical
minoxidil.
31
Over the years, we had accumulated a number of
women in our clinic who were either not satisfied with the
results achieved by conventional therapy, or who were intoler-
ant of topical minoxidil. Intolerance was either because of scalp
irritation or altered hair texture. Oral minoxidil is available in
Australia as 10 mg tablets. Off-label use of a half or quarter
tablet of oral minoxidil led to noticeable improvement in hair
density in most of these women but was complicated by postu-
ral hypotension, fluid retention, and hypertrichosis. While fluid
retention can often be managed by the addition of spironolac-
tone, this has the potential to increase postural hypotension.
As minoxidil side effects are all dose related, we com-
pounded oral minoxidil extemporaneously into capsules contain-
ing 0.25 mg or one-fortieth of a tablet.
To reduce the risk of fluid retention and to augment therapy
by the addition of an oral antiandrogen, spironolactone 25 mg
was added to the capsule. For women with low blood pressure,
50 mg of sodium chloride was also added to the capsule. The
combination of spironolactone and minoxidil is likely to have an
additive benefit in FPHL.
31
Low-dose oral minoxidil was well tolerated in the majority of
our patients with FPHL and is a reasonable alternative in
women intolerant of or unwilling to use topical minoxidil. While
hyperkalemia, creatinine elevation, and hepatitis are reported
with spironolactone,
32
we did not encounter any hematological
abnormalities at the dose used in this study.
Most women noticed a reduction in hair shedding at
3 months and an increase in hair density at 6 months.
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