Article

Boron and calcium chloride as possible ameliorators of fluoride toxicity in Wistar rats

Authors:
  • Indian Council of Agricultural Research National institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, India
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Abstract

Fluorosis is an endemic health problem for both humans and livestock in some parts of the world including India. This fast expanding problem needs remedial measures. Though limited reports suggest ameliorative effect of boron (B) and calcium (Ca) against fluoride (F) toxicity in animals, still there is a need for long duration study to understand the possible mechanism of action. Here, we did the same; studied the ameliorative effect of B and Ca on F toxicity in Wistar strain (Rattus norvegicus) rats. Forty-eight male Wistar albino rats were divided randomly into six groups. The group T1 was given normal water (control) while the groups T2-T6 were supplemented with F through water @ 30 ppm (as sodium fluoride), 30 ppm F+ 50 ppm B (as sodium borate), 50 ppm B, 30 ppm F+ 50 ppm calcium chloride and 50 ppm calcium chloride, respectively. After four months, all the rats were sacrificed and blood, teeth, femur bone and liver were collected and analyzed for mineral content. Fluoride toxicity significantly (P <0.05) reduced phosphorus content in bone, calcium content in liver, and superoxide dismutase (SOD) activity in plasma. However, F toxicity significantly (P <0.05) increased phosphorus and glucose content in plasma, and calcium and magnesium content in teeth and fluoride content in liver. Boron and calcium chloride as ameliorating agents significantly (P <0.05) reduced the fluoride content in all the internal organs and also significantly reduced the elevated level of glucose and phosphorus in plasma and improved SOD activity in plasma. It is concluded that toxic effect of F is partially ameliorated by supplementation of 50 ppm of B or CaCl2.

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Article
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Article
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Article
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Article
An experiment was undertaken to evaluate the protective role of boron on the serum profile of buffalo calves fed a high fluoride ration. Twelve male Murrah buffalo (Bubalus bubalis) calves of 6-8 months age, divided into three groups of four calves in each, were fed basal diets and supplemented with sodium fluoride (NaF, 60 ppm) alone or in combination with borax (Na2B4O7.10H2O, 140 ppm) for 90 days. Boron (B) was added in the ration as borax to make @140 ppm boron (elemental B) on DM basis in treatment II. Dietary F caused a significant (p<0.05) depressing effect on serum Ca and Zn on day 90 which was improved with B supplementation. However, serum Fe and Cu did not show any significant change on F or F+B supplementation. The serum ALP and phosphorus level were increased significantly (p<0.05) on F feeding but declined significantly (p<0.05) when B was fed. The findings suggested beneficial effect of boron on serum minerals and ALP in buffalo calves fed high fluoride ration.
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