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Abstract

Data from individual mice show that both CD4 and CD8 T cells appear in mice expressing a single TCRb chain regardless of the MHC allele expressed. The numbers of thymus and lymph node cells were counted and analyzed for their expression of CD4 and CD8 and T cell receptor.
Figure 1-source data 1
Thymus data
Mouse Strain Sex % DN % DP
1485 bb d- DOBWT BT1 F 22.95 3.06 2.17 72.5
1486 bb d- DOBWT BT1 F 29.49 3.94 5.11 79.3
1488 bb d- DOBWT BT1 M 17.43 4.97 8.43 76.2
Average 23.29 3.99 5.24 76.00
St err 3.49 0.55 1.81 1.97
st dev 4.93 0.78 2.56 2.78
1474  d- DOBWT BT2 F 28.36 7.24 4.12 84.3
1476  d- DOBWT BT2 F 26.53 5.16 4.64 83.3
1480  d+ DOBWT BT1 M 9.60 5.61 2.71 86.5
Average 21.50 6.00 3.82 84.70
St err 5.98 0.63 0.58 0.95
st dev 8.45 0.90 0.82 1.34
1461 ss d+ DOBWT F 24 15 6.68 81.2
1462 ss d+ DOBWT M 17 12 4.25 84.7
1464 ss d+ DOBWT M 16 7.3 5.2 83.1
Average 19.00 11.43 5.38 83.00
St err 2.52 2.24 0.71 1.01
st dev 3.56 3.17 1.00 1.43
1534 bb d+ DOBY48A M 5.85 4.50 2.44 92.7
1535 bb d+ DOBY48A M 13.98 3.06 2.68 93.9
1536 bb d+ DOBY48A M 11.39 5.20 2.5 94.3
Average 10.41 4.25 2.54 93.63
St err 2.40 0.63 0.07 0.48
st dev 3.40 0.89 0.10 0.68
1542  d- DOBY48A BT1 M 6.81 5.24 4.03 91.1
1543  d- DOBY48A BT1 M 5.02 1.67 3.77 91.4
1544  d- DOBY48A BT1 M 5.76 5.33 3.62 91
Average 5.86 4.08 3.81 91.17
St err 0.52 1.21 0.12 0.12
st dev 0.74 1.71 0.17 0.17
#
thymocyt
es (x10-7)
# LN cells
(x10-7)
1498 ss d+ DOBY48A M 13 9.4 7.55 87.8
1499 ss d+ DOBY48A M 12 8.5 5.83 90.1
1500 ss d+ DOBY48A M 7.7 7 6.72 88.7
Average 10.90 8.30 6.70 88.87
St err 1.63 0.70 0.50 0.67
st dev 2.30 0.99 0.70 0.95
Figure 1-source data 1
Thymus data
LN data
%CD4 % CD8 % CD4 % CD8
7.99 7.81 16.88801 0.275393 59.40 4.80 16.00
9.7 3.18 26.51704 2.747694 58.00 5.04 22.30
6.77 4.38 10.58708 1.343946 59.00 4.66 14.30
8.15 5.12 18.00 1.46 58.80 4.83 17.53
0.85 1.39 4.64 0.72 0.42 0.11 2.44
1.20 1.96 6.56 1.01 0.59 0.16 3.44
5.82 3.39 14.62274 2.470613 44.10 7.67 31.80
7.32 2.38 17.18537 2.74013 43.40 6.36 32.50
8.45 0.41 7.100366 0.271281 44.20 7.14 28.10
7.20 2.06 12.97 1.83 43.90 7.06 30.80
0.76 0.88 3.03 0.78 0.25 0.38 1.37
1.08 1.24 4.28 1.11 0.36 0.54 1.93
8.18 2.78 15.23443 5.20416 35.40 10.40 30.70
7.59 2.38 12.0256 3.4391 33.80 11.10 29.90
8.01 2.64 12.13675 3.455232 32.70 13.00 31.60
7.93 2.60 13.13 4.03 33.97 11.50 30.73
0.18 0.12 1.05 0.59 0.78 0.78 0.49
0.25 0.17 1.49 0.83 1.11 1.10 0.70
3.17 0.10 1.206768 0.028069 26.90 17.40 25.80
2.05 0.22 1.945924 0.163312 27.40 18.70 24.80
1.98 0.11 1.421038 0.053759 27.70 19.00 27.50
2.40 0.14 1.52 0.08 27.33 18.37 26.03
0.39 0.04 0.22 0.04 0.23 0.49 0.79
0.55 0.06 0.31 0.06 0.33 0.70 1.12
2.75 0.27 1.385754 0.065822 19.80 28.80 28.50
3.1 0.32 1.217767 0.105477 20.10 25.90 29.30
3.27 0.22 1.519072 0.074086 16.60 29.60 27.20
3.04 0.27 1.37 0.08 18.83 28.10 28.33
0.15 0.03 0.09 0.01 1.12 1.13 0.61
0.22 0.04 0.12 0.02 1.59 1.59 0.87
# CD4
TCRhi x
10-6
# CD8
TCRhi x
10-6
% of CD4
CD44hi
1.82 1.79 2.044224 1.694056 11.20 24.20 39.70
1.53 1.88 1.657908 1.53408 13.50 21.90 40.00
1.44 1.94 0.912542 0.973958 12.80 21.70 42.70
1.60 1.87 1.54 1.40 12.50 22.60 40.80
0.11 0.04 0.33 0.22 0.68 0.80 0.96
0.16 0.06 0.47 0.31 0.96 1.14 1.35
Figure 1-source data 1
LN data
5.87 78.10
4.66 82.10
10.30 81.00
6.94 80.40
1.72 1.19
2.43 1.69
10.90 2.07
6.71 0.79
12.60 0.87
10.07 1.24
1.75 0.41
2.48 0.59
7.18 0.43
7.18 0.84
9.44 0.86
7.93 0.71
0.75 0.14
1.07 0.20
28.40 94.40
16.10 89.00
27.00 93.10
23.83 92.17
3.89 1.63
5.50 2.30
17.00 0.87
16.50 0.62
21.00 0.78
18.17 0.76
1.43 0.07
2.02 0.10
% of CD8
CD44hi
% CD4-
CD8- that
are Kbpos
17.30 1.35
15.40 0.58
13.40 0.34
15.37 0.76
1.13 0.31
1.59 0.43

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