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Supplementary file 2.

November 2017

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CC BY 4.0

Authors:

Philippa Marrack

National Jewish Health

University of Colorado

National Jewish Health

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Sequences of TCRβ transgenes

Available via license: CC BY 4.0

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Supplementary File 2

Name TRBV (Arden nomenclature) CDR3

CASGSGTTNTEVFF

CASGSGTTNTEVFF

Sequences of TCRb transgenes

DObWT 13.2 (Vb8.2)

DOb48A 13.2 (Vb8.2 with Y48 mutated to A)

Supplementary File 2

TRBJ

1.1

1.1

elife-30918-supp

Office Document

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The somatically generated portion of the T cell receptor CDR3α contributes to the MHC allele specificity of the T cell receptor

Article

November 2017

Philippa Marrack · Harsha Krovi · Daniel Silberman · Janice White · John Kappler

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Data

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Additional file 2

June 2012

Sequences of 24 nucleotide small RNAs mapping specifically to each d35S in the two transgenes. Small RNAs were classified based on their transgene origin and strandedness. The sequences of the small RNAs, the number of reads and the positions of their 5′ nucleotides along the construct as shown in Additional file 1: Figure S4 is shown.

Data

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Supplementary file 1.

March 2018

Sequences of the various MS2bp-GFP-nls transgenes used is the experiment in Figure 1. Each sequence is split into three: the 5’ sequences, the open reading frame in bold, and 3’ sequences.

Data

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Additional File 1

November 2008

Sequences of DNA stuffer used in the study. this file provides all the transgene sequences used in the study.

Data

Full-text available

Supplementary file 1.

November 2017

In normal mice, a significant number of TCRα sequences appear on naïve CD4 T cells regardless of the selecting MHCII allele. Naïve CD4 T cells were isolated from the lymph nodes of normal mice of the indicated strains and their TCRα sequences identified as described in the Materials and methods section. Shown are the %s of unique sequences and the %s of total sequences that were shared between ... [Show full abstract] pairs of mice of the indicated strains. Data were obtained from three independently sequenced B6 mice and one each B6.AKR and B6.NOD animals and are the means and standard errors of the means of the comparisons.

Data

Full-text available

Figure 2—source data 1.

November 2017

After transduction with the DObWT chain, T cells from mice expressing DOb48A react with cells bearing the MHC allele that selected them. CD4 T cells were isolated from individual DOb48A mice and transduced with retorviruses expressing DObWT or DOb48A as described in the Methods section. The cells were vultured with spoleen cells from mice expressing various MHC alleles and responses assessed one ... [Show full abstract] day later by measuring the % of cells that bore CD69. Data shown are from individual mice assayed on different days.

Data

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Transparent reporting form

November 2017

Data

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Figure 1—source data 1.

November 2017

Data from individual mice show that both CD4 and CD8 T cells appear in mice expressing a single TCRb chain regardless of the MHC allele expressed. The numbers of thymus and lymph node cells were counted and analyzed for their expression of CD4 and CD8 and T cell receptor.

Last Updated: 24 Jan 2023