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Beneficial Effects of Collagen Hydrolysate: A Review on Recent Developments

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Research Article Open Access


11,2*
1Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, China
2Beijing Higher Institution Engineering Research Center of Animal Product, China
 July 15, 2017;  July 24, 2017
 Bo Li, Beijing Higher Institution Engineering Research Center of Animal Product, Beijing Advanced Innovation Center for Food
Nutrition and Human Health, 17, Qinghua East Road, Haidian District, Beijing 100083, China, Tel: ; Email:
Introduction
Collagen is the main structural protein of the different
connective tissues, such as skin, bone, cartilage and tendons, and
comprises about one-third of total proteins in mammals. Collagen
extracted from collagen-rich materials with hot water is known
as gelatin. The common materials used for extracting gelatin
include pig skin (46%), bovine hide (29.4%), bones (23.1%)
         
great attention in recent years due to the religions, cultures and
health concerns. The further enzymatic hydrolysis of gelatin
results in collagen hydrolysate (CH). CH has long been used in
pharmaceuticals and foods in many countries and regions, such
as United States, Europe, China and Japan. Approved as Generally
 
the Food and Drug Administration (FDA) Center for Food Safety and
Nutrition. The bioavailability and absorption of CH have also been
widely studied. It has been reported that CH is more easily absorbed
and has higher bioavailability than gelatin [2,3]. Besides free amino
acids, small peptides especially dipeptide and tripeptide are also
absorbed into body by peptide transporter 1 (PepT1) [4]. To date,
more than30 peptides (mainly dipeptides and tripeptides) have

the most abundant collagen-derived peptide [5-7]. These peptides
         
studies reported [8-9]. In this paper, we will mainly focus on the

CH in animal experiments and clinical trials (Figure 1). The possible

Figure 1: The benecial effects of CH in animal experiments
and clinical trials.

Tech Res 1(2)-2017. BJSTR. MS.ID.0002017.DOI: 10.26717/BJSTR.2017.01.000217
Abstract
Collagen hydrolysate (CH) has received increasing attention in recent decades. This review mainly summarizes the recent developments

 
anti obesity and hypoglycemic effects. We also make comments on the current researches and give suggestions for future studies in this review.
       
guidance to develop CH-based health care supplements for disease prevention and/or treatment.
 
DOI: 10.26717/BJSTR.2017.01.000217
Bo Li.

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Submission Link: http://biomedres.us/submit-manuscript.php
Bo Li. Biomed J Sci & Tech Res Volume 1- Issue 2: 2017
Benecial Eects

CH itself has good antioxidant activity as demonstrated by
in vitro assays [10,11]. CH intake also increases the activities of
antioxidant enzymes in body, including SOD, GSH-Px and CAT
[3,12]. Nuclear factor E2-related factor 2 (Nrf2)-antioxidant
response element (ARE) pathway plays a central role in regulating
antioxidant enzymes. Therefore, we speculate that CH exerts it
antioxidant effect in a direct and/or indirect manner. More work is
needed to investigate the effect of CH on Nrf2-AREsignaling.

Anti aging effect of CH has been widely investigated in several
animal models, including photo aged model, chronologically aged
model and acetone-induced dry skin model [3,12-15]. General
      
the formation of deep wrinkles and improving skin elasticity, are
also observed in clinical trials after taking 10g of CH once a day
for more than 6 weeks [16]. It should be noted that anti aging
effect of CH is more obvious on women aged more than 30 years.
       
the dual effects of CH on skin collagen synthesis and degradation,
as previous study reported [13].

Anti osteoporotic and anti osteoarthritis effects of CH have been


the symptoms of osteoarthritis and osteoporosis [18]. Combined
CH with other nutritional ingredients has received much interest.
It has been reported that combined oral administration of CH with
calcium and vitamin D has better effects on bone health than alone
administration of CH or calcium and vitamin D [19-21]. Future
studies are needed to determine the optimal form and optimal dose
of CH.

Oral administration of marine CH is reported to enhance
cutaneous wound healing and angiogenesis in rats [22-23]. In
addition to elevated VEGF and FGF-2 expression, the effect of CH
 
wound healing, as previous study reported that Pro-Hyp, a collagen-
        


Glycine is one of the major structural units of collagen,
accounting for one-third of the amino acids.CH has the ability
        
      
cytokine via glycine-gated chloride channels (GlyR).

Liang et al. reported CH intake inhibited spontaneous tumor
incidence and increase life spanin sprague-dawley (SD) rats
[26]. Our previous study found that CH intake inhibited platelet
release and down regulated proangiogenic factors in blood [27].
Considering angiogenesis is of key importance in the process of
tumorprogression. Our results provide a possible mechanism
underlying antitumor effect of CH.

Antihypertensive effect of CH has been reported in animal
experiments and clinical trials [28,29]. Oral administration of
  
pressure by inhibiting angiotensin I converting enzyme (ACE)
[28]. Tang et al. [30]found that oral administration of collagen
tripeptide had an inhibitory effect on atherosclerosis development
in hypercholesterol emic rabbits [30]. Platelets are involved in the
patho physiology of atherosclerosis and thrombosis.CH has an
inhibitory activity on platelet release and platelet aggregation, which
may justify potential application of CH as a health care supplement
to prevent and/or treat atherosclerosis and thrombosis-related
cardiovascular diseases [27].

CH intake has an effect on the absorption and metabolism of

weight gain and down regulates serum levels of total cholesterol,
triglyceride and low-density lipoprotein [31]. Further, CH could
alter lipid metabolism-related gene expression and the unfolded
protein response in mouse liver [32]. The hypoglycemic effects
of CH have also been reported [33,34]. It has been reported that
CHcan improve glucose tolerance by inhibiting intestinal glucose
uptake and enhancing insulin secretion [34], suggesting the anti
diabetic property of CH.
Conclusion and Perspective

effects on body health. Those effects make CH new and potential
healthcare supplement for disease prevention and/or treatment
in pharmaceuticals and foods. However, several issues should be
noted and need to be further explored. First, many in vitro studies
only focus on the biological activities of CH, neglecting its tolerance
in gastrointestinal tract. Certain collagen peptides isolated in in
vitro studies may be hydrolyzed by gastrointestinal enzymes and
exert no biological activity in body as expected in in vitro studies.
In vitro simulated GI digestion and Caco-2 mono layers have been
widely used and allow the prediction of oral compounds digestion
       
effects of CH have been reported, what is the peptide sequence
       
activity relationship will guide the application of CH. Third, the
biological activities of protein hydrolysates are highly affected by
their molecular structure and weight, which are greatly impacted
by their processing conditions. Therefore, processing conditions

effect.
Acknowledgement
This study was supported by the grants from China Agriculture
Research System (CARS-46) and National Natural Science
Foundation of China (NSFC,No. 31271846).
3/4
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Bo Li. Biomed J Sci & Tech Res Volume 1- Issue 2: 2017
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... Gelatin is a water-soluble fibrous collagen hydrolysate with a high molecular weight ranging from 97 to 250 kDa (Atma et al., 2018;Mulyani, Setyabudi, Pranoto, & Santoso, 2017;Rakhmanova, Khan, Sharif, & Lü, 2018;Sanaei, Mahmoodani, See, Yusop, & Babji, 2013). Collagen is a major structural protein of connective tissues present in blood vessels, cartilage, skin, tendons, ligaments, and bones (Alves, Marques, Martins, Silva, & Reis, 2017;Hashim, Mohd Ridzwan, Bakar, & Mat Hashim, 2015;Song & Li, 2017). Collagen is found in most mammals, accounting for approximately 25-30% of their total protein (Alves et al., 2017;Hashim et al., 2015;Song & Li, 2017). ...
... Collagen is a major structural protein of connective tissues present in blood vessels, cartilage, skin, tendons, ligaments, and bones (Alves, Marques, Martins, Silva, & Reis, 2017;Hashim, Mohd Ridzwan, Bakar, & Mat Hashim, 2015;Song & Li, 2017). Collagen is found in most mammals, accounting for approximately 25-30% of their total protein (Alves et al., 2017;Hashim et al., 2015;Song & Li, 2017). Since gelatin is produced through partial hydrolysis of collagen, the quality of the gelatin depends on the source of the collagen. ...
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... However, in other species, the bioactive amino acids and peptides in PHC stimulated gastric mucus production, formed a barrier over ulcerated tissues and increased pH within the glandular ulcer bed, resulting in resistance of the tissue to acid degradation (Ohara et al. 2007;Shaw et al. 2016). In addition, collagen hydrolysates were shown to have anti-inflammatory and antioxidant activity in vitro (Aleman et al. 2011;Nakchum and Kim 2016;Song and Li 2017). Antioxidant and immunomodulary properties are important in glandular mucosal healing in other species (Das and Banerjee 1993;Xu et al. 2007) and might have contributed to reduction in the number of nonglandular ulcers in the study reported here. ...
... Antioxidant and immunomodulary properties are important in glandular mucosal healing in other species (Das and Banerjee 1993;Xu et al. 2007) and might have contributed to reduction in the number of nonglandular ulcers in the study reported here. Furthermore, glycine and proline both locally and systemically have antioxidant effects by regulating the nuclear factor erythroid 2-related factor 2(Nrf2)-antioxidant response element (ARE) pathway, which might have played a central role in regulating antioxidant enzymes and protected the gastric tissue against oxidative damage triggered by inflammation (Song and Li 2017). ...
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... It is the main component of tissues such as fibrous tissue, bone, cartilage, valves of heart, cornea, and basement membrane etc. 1,4,6,16 ...
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... Gelatin is extracted from collagen in hot water [84]. It shows various beneficial effects like anti-oxidant, anti-aging, antitumor, wound healing, anti-inflammatory, antihypertensive, antiatherosclerotic, anti-obesity, hypoglycemic, antiarthritic etc in different animal and clinical studies [85]. The main mechanism of anti-arthritic action is the formation of new collagen type II and stimulation of proteoglycan synthesis [86]. ...
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Collagen tripeptide (CTP) and galacto‐oligosaccharide (GOS), which improve collagen homeostasis and barrier function in the skin, are widely used in the food industry to improve wrinkle‐related parameters and skin health. In this study, the photoprotective effect of CTP/GOS mixtures (3:1, 1:1, and 1:3) in ultraviolet (UV) B‐irradiated hairless mice was examined. Skin parameter analysis, histological approaches, molecular biology techniques and HPLC analysis were applied to investigate the photoaging protective effect, signaling pathways and changes in the microbiota. Oral administration of CTP/GOS mixtures ameliorated photoaged physical parameters and serum levels of pro‐inflammatory cytokines compared to UV‐irradiated control group. Administration of the 1:3 mixture showed significant changes in the extracellular matrix‐related gene expression compared to other mixture groups. The cecal short chain fatty acid (SCFA) content showed a significant increase in the CTP/GOS mixed group with a higher GOS content than the control group. In the 16S rRNA‐based analysis of cecal microbiota, the relative abundance ratio of the Akkermansia genus belonging to the Verrucomicrobia phylum was higher in CTP and GOS mixture‐administered groups than in the UV‐irradiated control group. Taken together, CTP/GOS mixtures showed a synergistic effect on photoprotective activity through changes in the gene expression, cytokine levels, and intestinal microbiota composition.
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GIVING COLLAGENT SUPPLEMENTS ON BLOOD SUGAR LEVELS IN MENOPAUSE WOMEN Background: Menopause is a natural process experienced by every woman aged around 48-54 years. The factors that affect menopause at the beginning are usually due to indiscriminate food consumption so that menopausal women experience diabetes mellitus which is increasing where it is known that blood sugar levels can increase.Purpose: This study was to determine the effect of collagen supplementation on blood sugar levels in menopausal women suffering from Diabetes Mellitus in the Work Area of the Curug Public Health Center, Tangerang Regency, 2020.Method: This study was a quasi-experimental study with a One Group Pretest-Postest Design without a control group. The sampling technique used was total sampling. The sample in this study consisted of 20 menopausal women who suffered from diabetes mellitus in June-July 2020.Results: the results of this study indicated that blood sugar levels in menopausal women before being given collagen supplements were 277.15 and after being given collagen supplements 260.30. The results of the Paired t-test analysis showed that there was no effect of blood sugar levels in menopausal women suffering from diabetes mellitus, before and after giving collagen supplements with a value of Pvalue = 0.471.Conclusion : the conclusion is that there is no effect of blood sugar levels before and after giving collagen supplements to menopausal women suffering from diabetes mellitus in the Work Area of the Curug Public Health Center, Tangerang Regency, 2020Suggestion: It is hoped that further research can carry out further research on collagen supplementation in menopausal women on blood sugar levels with a longer / longer study time and add other variables that have not been studied. Keywords: Menopause, Blood Sugar, Collagen. ABSTRAKLatar Belakang: Menopause merupakan proses alamiah yang dialami setiap wanita yang berusia sekitar 48-54 tahun, faktor yang mempengaruhi menopause pada awal biasanya dikarenakan konsumsi makanan yang sembarangan sehingga wanita menopause mengalami diabetes mellitus semakin meningkat dimana telah ketahui kadar gula darah dapat meningkat.Tujuan : Penelitian ini untuk mengetahui pengaruh pemberian suplemen kolagen terhadap kadar gula darah pada wanita menopause yang menderita Diabetes Mellitus di Wilayah Kerja Puskesmas Curug Kabupaten Tangerang Tahun 2020.Metode : Penelitian ini quasi-experiment dengan rancangan One Group Pretest-Postest Design tanpa adanya kelompok kontrol.Teknik pengambilan sampel yang digunakan adalah total sampling. Sampel dalam penelitian ini berjumlah 20 wanita menopause yang menderita diabetes mellitus pada bulan juni-juli tahun 2020Hasil penelitian ini menunjukkan bahwa kadar gula darah pada wanita menopause sebelum diberikan suplemen kolagen 277,15 dan sesudah diberikan suplemen kolagen 260,30. Hasil analisis Uji Paired t-test menunjukan bahwa tidak ada pengaruh kadar gula darah pada wanita menopause yang menderita diabetes mellitus,sebelum dan sesudah pemberian suplemen kolagen dengan nilai Pvalue =0,471.Kesimpulan tidak ada pengaruhkadar gula darah sebelum dan setelah pemberian suplemen kolagen pada wanita menopause yang menderita diabetes mellitus di Wilayah Kerja Puskesmas Curug Kabupaten Tangerang Tahun 2020Saran : diharapkan untuk penelitian selanjutnya dapat melakukan penelitian lebih lanjut mengenai suplemen kolagen pada wanita menopause terhadap kadar gula darah dengan waktu penelitian yang lebih panjang/lamadan menambahkan variable-variabel lain yang belum di teliti. Kata Kunci :Menopause, Kadar Gula Darah ,Suplemen Kolagen
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The use of collagen hydrolysates (CHs) as a nutraceutical agent in skin aging has gained increasing attention. Here, the effects of various doses and molecular weights of CH from silver carp skin on photoaging in mice were investigated. The ingestion of CH at 50, 100 and 200 mg per kg body weight led to a dose-dependent increase in the hydroxyproline, hyaluronic acid and moisture contents of the skin, but it had no significant effect on the mice body weight, or on the spleen or thymus index. Furthermore, ingesting CH with lower (LMCH, 200-1000 Da, 65%) and higher molecular weight (HMCH, >1000 Da, 72%) significantly increased the skin components and improved the antioxidative enzyme activities in both serum and skin (p < 0.05); LMCH performed better than HMCH. By contrast, gelatin (>120 kDa) ingestion did not bring a significant change compared to model mice. These results indicated that LMCH exerted a stronger beneficial effect on the skin than did either HMCH and gelatin, which supported the feasibility of using LMCH as a dietary supplement from silver carp skin to combat photoaging.
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Introduction Collagen hydrolysate is recognized as a safe nutraceutical, whose combination of amino acids stimulates the synthesis of collagen in the extracellular matrix of cartilage and other tissues. Objective to conduct a systematic review of literature on the action of collagen hydrolysate in bone and cartilaginous tissue and its therapeutic use against osteoporosis and osteoarthritis. Method a study of the PubMed, MEDLINE, LILACS, and SciELO databases was performed. Articles published in English and Portuguese in the period of 1994 to 2014 were considered. Results: the sample comprised nine experimental articles with in vivo (animals and humans) andin vitro (human cells) models, which found that the use of different doses of collagen hydrolysate were associated with the maintenance of bone composition and strength, and the proliferation and cell growth of cartilage. Conclusion hydrolyzed collagen has a positive therapeutic effect on osteoporosis and osteoarthritis with a potential increase in bone mineral density, a protective effect on articular cartilage, and especially in the symptomatic relief of pain.
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