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Effects of Fulvic Acid on Different Cancer Cell Lines

DOI:10.3390/proceedings1101031
Authors:
Safa Kerem Aydın at Middle East Technical University
Safa Kerem Aydın
Seher Dalgic
Seher Dalgic
Seher Dalgic
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Merve Karaman
Merve Karaman
Merve Karaman
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Ömer faruk Kırlangıç at Gazi University
Ömer faruk Kırlangıç
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Abstract

Humic substances are commonly found in decaying organic matter including plants, animal residues, sewage and soil. Although fulvic-acid accounts for ∼90% of all substances and its biological significance recognized for many years, there is still minimal scientific understanding the claims of its biological properties. However, these formulations contain numerous toxic elements that make their use clinically impossible. Recently, there has been development of a pure form of fulvic-acid, carbohydrate derived fulvic-acid (CHD-FA) that has been shown to be safe to use clinically and absent from environmental contaminants known to be harmful to the host. The aim of this study is to evaluate the effects of fulvic-acid on different cancer cells. Hep3B, HT29 and PC3 cells were treated different concentrations fulvic-acid for 48 and 72 h and cell proliferation was performed MTT test. Changes in the mRNA levels of apoptotic genes were also analyzed in PC3 cells. Fulvic-acid inhibited the proliferation of all the cell lines used in this study determined by MTT analysis. Specifically, Hep3B cells were found to be most sensitive for 48-hour application with 1.58–2.43 μg/μL IC50 value. Additionally, fulvic-acid significantly upregulated the apoptotic genes at mRNA levels compared to non-treated control group.
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Proceedings 2017, 1, 1031; doi:10.3390/proceedings1101031 www.mdpi.com/journal/proceedings
Abstract
Effects of Fulvic Acid on Different Cancer Cell Lines †
S.Kerem Aydin 1, Seher Dalgic 1, Merve Karaman 2, O.Faruk Kirlangic 3 and Hatice Yildirim 3,*
1 Sirri Yircali Anatolian High School, Balikesir 10020, Turkey
2 Department of Biology, Balikesir University, Balikesir 10145, Turkey
3 Department of Molecular Biology and Genetic, Balikesir University, Balikesir 10145, Turkey
* Correspondence: hbozkurt@balikesir.edu.tr or hatice_dna@hotmail.com; Tel.: +90-266-612-10-00-1122
† Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy,
Kayseri, Turkey, 8–11 November 2017.
Publish: 14 November 2017
Abstract: Humic substances are commonly found in decaying organic matter including plants,
animal residues, sewage and soil. Although fulvic-acid accounts for ∼90% of all substances and its
biological significance recognized for many years, there is still minimal scientific understanding the
claims of its biological properties. However, these formulations contain numerous toxic elements
that make their use clinically impossible. Recently, there has been development of a pure form of
fulvic-acid, carbohydrate derived fulvic-acid (CHD-FA) that has been shown to be safe to use
clinically and absent from environmental contaminants known to be harmful to the host. The aim
of this study is to evaluate the effects of fulvic-acid on different cancer cells. Hep3B, HT29 and PC3
cells were treated different concentrations fulvic-acid for 48 and 72 h and cell proliferation was
performed MTT test. Changes in the mRNA levels of apoptotic genes were also analyzed in PC3
cells. Fulvic-acid inhibited the proliferation of all the cell lines used in this study determined by
MTT analysis. Specifically, Hep3B cells were found to be most sensitive for 48-hour application with
1.58–2.43 μg/μL IC50 value. Additionally, fulvic-acid significantly upregulated the apoptotic genes
at mRNA levels compared to non-treated control group.
Keywords: fulvic-acid; cancer; apoptosis
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons
Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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