Available via license: CC BY 4.0
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Table S3. 2D-gel analysis of proteins from cells grown at all steady-states from 25% - 345% aerobiosis. In the “All
steady-state raw data” tab, the normalised spot volumes from image analysis for a given protein are shown for multiple gels
run with a minimum of 1 steady-state per oxygen input condition (50% and 75% aerobiosis) or with 2 or more independent
steady-states (25, 40, 88, 100, 150, 200 and 345% aerobiosis). CV refers to coefficient of variation. The data are sorted by
the ANOVA value. Note that some proteins appeared as multiple spots and so may have more than one entry. For each
aerobiosis condition the spot volumes for a given protein on each gel were averaged and these values are shown in the
“mean spot volumes” tab. These mean values were used to construct the graphs of changes in selected protein abundance
shown in the paper.
Anova (p) Included
2D-gel
spot#
Fold-
change
between
highest
and lowest
mean
Identication/annotation
!!"#$%&!' !(
!!"#! !&!' !)
!!"#$%&!' !(
&!''&!*"&*
#+!'",#*&!-.
&!*""/01")
2##(3''#4! !,
!!'!&!&!(
&!*""/01")
&!''&!*"&*
#!"*!"#!*)
#&!''()&#"!&!!&!&#'",&!&'(
&!''&!*"&*
&!*""/01")
#&!''()&#"!&!!&!&#'",&!&'(
#(#"!.5#)
# 6&!,
(7"!&!*"!"#.5&
&!*""/01")
&!&(
#!%""&!.
7"!&!*"!"#&
#!"*!"#!*)
#(##8(#(
# 6&!,
#" !/4 2&!,1.5#2
+%9:*"!"#'
###:
!!"#$%&!' !(
(&!.5.&(
#(##8(#(
, #!9.2!509.! !,
#+!%$%"**"!"#.5.
&!&!&9.
#(#"!.5.!;
#+%#!#!8"&!
2!'#.5*2
#4<0"!,#"(
;"""!,=
&%*"!"#'
#+%! !
4!!,>!#&?<:@<
#(#"!.5.
#+%:+&,!"#&!
+%&!&#*"!"#
#A&!
&#&!
#!'!B'!#'",&!#"0
CCD' *,!##&!*"!"#!
#)&!!,*!&&!#)
#+%:+&,!"#&!
CCD(' *,!##&!*"!"#!(
""!,"
*"!"#
0!!"#! !&! !")
#+%:+&,!"#&!
3 !"(3 !!"#$%&!'
#:")&!,
#+%(>+<9>+'",&!
#6'##!#!"#! !'.5.&(
+!&#!!"#.5&(
#(7!!&#,&%&!&#&!
#*"!"#
#) #&(&!
&%&!&#&!
#?#D4(.52
+&&7
3 !"(3 !!"#$%&!'
#9D4(9"! !'),)
9!&&!/;2+# #!,!&
*#E##!!!&!F!"# '9
#+%#!#!8"&!.
#&''#! !!
""!"&#"&(
+%&"!'
#&!""""!,"
7=#&!/#&1,!A
=4("!#"D4(&!.5!&)
=4("!#"D4(&!''.
#) #&(&!3&%.!;
2##(,E(=+ !,F'&#=
,#D4(&#,G
CCD(' *,!##&!*"!"#!(
#7#7(
#+!%$%"**"!"#.5.
! !,/:-&?1
345% aerobiosis (17% Oxygen)
Max CV (%) Highest Mean Lowest Mean
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
Normalized protein spot volume
2"
9)
2"
9
2"
9(
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
HC*, HC*,
345% aerobiosis (17% Oxygen)
200% aerobiosis (10% Oxygen)
Normalized protein spot volume
2"
9
2"
9(
2"
9)
2"
9
2"
9(
2"
9(
200% aerobiosis (10% Oxygen)
150% aerobiosis (7.5% Oxygen)
2"
9(
2"
9)
2"
9(
2"
9
2"
9)
2"
9(
2"
9(
150% aerobiosis (7.5% Oxygen)
2"
9(
2"
9)
2"
9)
2"
9)
2"
9
2"
9
2"
9(
100% aerobiosis (5% Oxygen)
2"
9(
2"
9)
2"
9
2"
9(
2"
9(
2"
9)
100% aerobiosis (5% Oxygen)
88% aerobiosis (4.38% Oxygen)
2"
9(
2"
9(
2"
9(
2"
9)
2"
9)
75% aerobiosis (3.75% Oxygen) 50% aerobiosis (2.5% Oxygen)
2"9
(
2"9
)
2"
9(
2"
9)
2"
9
40% aerobiosis (1.88% Oxygen)
2"
9(
2"
9(
2"
9)
2"
9
2"
9)
2"
9(
40% aerobiosis (1.88% Oxygen)
2"
9)
2"
9(
2"
9(
2"
9)
2"
9(
2"
9)
40% aerobiosis (1.88% Oxygen)
25% aerobiosis (1.25% Oxygen)
Tags
Edited
*
*
*
*
*
*
*
*
*
*
*
*
*
* *
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
* *
*
2"
9(
2"
9(
2"
9)
2"
9(
Highly
Signican
t (p<10e-
5)
between
1.88%,
7.5% and
17%
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
* *
*
*
*
*
*
*
*
*
*
*
*
*
Tags
* * * * *
* * * * *
* * * * *
*******
* * * * *
* * * * *
* * * * *
* * * * *
* * * * *
* * * * * *
*******
*******
* * *
* ****
* * * * *
* * * * *
* * * * *
* ****
* * * * * *
* * * * * *
* * * * *
* * * * *
* * * * *
* * * * *
* * *
* ****
* ****
* ****
* * * * *
* * * * *
* *
* * *
* * *
* * * * *
* * * *
* * *
* *
*******
*
* * *
* ****
Not used
in
analysis
Highly
Signican
t (p<10e-
5)
between
1.88%,
and 7.5%
Highly
Signican
t (p<10e-
5)
between
7.5%, and
17%
>2 fold
and
Highly
Signican
t (p<10e-
5)
between
1.88%,
and 7.5%
>2 fold
and
Highly
Signican
t (p<10e-
5)
between
7.5% and
17%
>2 fold
and
Highly
Signican
t (p<10e-
5)
between
1.88%
7.5% and
17%
Highly
Signican
t (p<10e-
5)
between
1.88% and
17%
>2 fold
and
Highly
Signican
t (p<10e-
5)
between
1.88% and
17%
* * * * * *
*
*
* * *
* * * * *
* *
* *
***
*****
* * *
* * *
*
* * * * *
* *
* * *
*
* * *
*
* * * *
***
* * * * *
* *
* * *
*
* * *
*
* *
*
*
* * *
* * * *
Tags
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
Highly
Signican
t (p<10e-
5) All
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
Prole
Spot #
Comment
Normalized Volume (means from all gels at each
oxygen condition)
Identicatio
n
17%
Oxygen
10%
Oxygen
!
!"#
$%&!
' !(
!
!"#!
!&!
' !)
!
!"#
$%&!
' !(
&!''
&!*"&*
#
+%
! !
4
!
!,
>!#
&?<:@<
#+!
'",
#*
&!-.
&!*"
"/01
")
2##(
3'
'#
4!
!,
!
!
'!
&!&!(
&!*"
"/01
")
&!''
&!*"&*
#
!"*
!"#!*)
#
&!''()
&
#"
!&!!
&!&#
'",
&!&'(
&!''
&!*"&*
&!*"
"/01
")
#
&!''()
&
#"
!&!!
&!&#
'",
&!&'(
#
(#
"!.
5
#)
# 6
&!
,
(7
"!&!*"
!"#.
5
&
&!*"
"/01
")
&!
&(
#
!%"
"&!.
7
"!&!*"
!"#
&
#
!"*
!"#!*)
#(#
#8(
#(
# 6
&!
,
#
" !
/4 2&!
,1.
5#2
+%9:
*"!"#
'
#
#
#:
!
!"#
$%&!
' !(
(&!
.
5.
&(
#(#
#8(
#(
,
#!9.
2!509.
!
!,
#
+!%
$%"*
*"!"#.
5.
&!
&!&9.
#
(#
"!.
5.
!;
#
+%
#!#!8
"&!
2
!'#
.
5
*2
#4<0
"!,
#
"(
;"
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=
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5.
#
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&,
!"#
&!
+%
&!&#
*"!"#
#A
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&#
&!
#!
'!
B'!#
'",&!
#"0
CCD
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*,!#
#&!
*"!"#
!
#
)&!
!,*
!&
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#
+!%
$%"*
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5.
!
!,
/:-
&?
1
#
+%:+
&,
!"#
&!
CCD(
'
*,!#
#&!
*"!"#
!(
""!,
"
*"!"#
0!
!"#!
!&!
!")
#
+%:+
&,
!"#
&!
3 !"(3
!
!"#
$%&!
'
#:")
&!
,
#
+%
(>+<9>+
'",&!
#
6'
##!#
!"#!
!'.
5.
&(
+!&#
!
!"#.
5
&(
#
(7!!&
#,
&%
&!&#
&!
#
*"!"#
#
) #
&(&!3
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&%
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&!
#
?#D4(
.
52
+&&
7
3 !"(3
!
!"#
$%&!
'
#9
D4(9
"! !
')
,)
9!&
&!/;2+
# #!,!&
*#E#
#!!!&!F
!"# '9
#
+%
#!#!8
"&!.
#
&'
'#! !!
""!"&#
"&(
+%
&
"!
'
#
&!
""
""!,
"
7=
#&!
/#&1,!A
=4(
"!#"D4(
&!.
5
!&)
=4(
"!#"D4(
&!
''.
#
) #
&(&!3
&%.
!;
2##
(,
E(=+ !,F
'&
#=
,#
D4(
&#
,G
CCD(
'
*,!#
#&!
*"!"#
!(
#
7#7(
Normalized Volume (means from all gels at each
oxygen condition)
7.5%
Oxygen
5%
Oxygen
4.38%
Oxygen
3.75%
Oxygen
2.5%
Oxygen
1.88%
Oxygen
Tags
Edited
*
*
*
*
*
*
1.25%
Oxygen
17%
Oxygen_x0
00D_V_x00
0D_10%
Oxygen
10%
Oxygen
V
7.5%
Oxygen
7.5%
Oxygen
V
5% Oxygen
Highly
Signicant
(p<10e-5)
between
1.88%,
7.5% and
17%
*
*
*
*
*
*
*
* *
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
* *
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
*
* *
*
*
*
*
*
*
*
*
*
*
*
*
Tags
***
***
***
* * * * *
***
***
Not used
in analysis
Highly
Signicant
(p<10e-5)
between
1.88%,
and 7.5%
Highly
Signicant
(p<10e-5)
between
7.5%, and
17%
>2 fold
and Highly
Signicant
(p<10e-5)
between
1.88%,
and 7.5%
>2 fold
and Highly
Signicant
(p<10e-5)
between
7.5% and
17%
>2 fold
and Highly
Signicant
(p<10e-5)
between
1.88%
7.5% and
17%
***
***
***
* * * *
* * * * *
* * * * *
***
* * *
***
***
***
* * *
* * * *
* * * *
***
***
* * *
* * *
***
* * *
* * *
* * *
***
***
*
***
***
***
* * * *
***
*
* * * * *
*
* * *
* * *
* * * *
*
*
***
***
*
*
*
* * * * *
***
***
***
*
***
*
***
* * * *
*
***
*
***
***
*
*
*
***
* *
Tags
* * *
* * *
* * *
* * *
* * *
* * *
Highly
Signicant
(p<10e-5)
between
1.88% and
17%
>2 fold
and Highly
Signicant
(p<10e-5)
between
1.88% and
17%
Highly
Signicant
(p<10e-5)
All
* * *
* * *
* * *
* * *
* * *
* * *
*
* * *
* * *
* * *
* * *
* * *
* * *
* * *
* * *
* * *
* * *
* * *
*
* * *
* * *
* * *
* * *
* * *
* *
*
*
* * *
*
*
* *
* * *
*
*
* * *
* * *
*
*
*
* * *
* *
* *
* * *
*
*
*
* *
* * *
* *
*
*
*
* *
*
* * *
* * *
* *
*
* *
*
*
* *
*
* *
*
*
*
*
*
*
*
*
*
*
* * *
*
*
*