Aim: To evaluate the anti- microbial activity of jojoba oil against selected microbes. Objective: The study is to determine the anti-microbial activity of jojoba oil against selected microbes. Background: Jojoba oil is the liquid produced in the seed of the Simmondsia chinensis. It is used as a replacement for whale oil and its derivatives, such as cetyl alcohol. It is found as an additive in many cosmetic products, especially those marketed as being made from natural ingredients. In particular, such products commonly containing jojoba are lotions and moisturizers, hair shampoos and conditioner. Effect of jojoba oil on E. coli, pseudomonas species, klebsiella species and Staphylococcus aureus is determined. Reason: This study is to evaluate the anti microbial activity of jojoba oil against E, coli, pseudomonas species, klebsiella species and Staphylococcus aureus. This may help in the development of other products with jojoba oil as its constituent. Result: The investigation of anti microbial activity of jojoba oil on selected positive and negative bacilli was done by agar well diffusion technique and its zone of inhibition was evaluated.