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September 2017 | Volume 30 | Issue 3 | Page 1
Pakistan Journal of Agricultural Research
Research Article
Introduction
Basil (Ocimum basilicum L.) is native plant of trop-
ical regions. It belongs to genus Ocimum which
comprises between 50 and 150 species of herbs and
shrubs (Paton et al., 1999). It is one of endemic plant
which is used not only as pharmaceutical plant but
also used as culinary herb (Naghibi et al., 2005).
Seeds of basil have been used as traditional medicine
for treatment of dyspepsia, ulcer, diarrhea and oth-
er illness. Many parts of Asia basil seeds are used to
prepare traditional beverages (sharbat) and many ice
desserts like falooda (Hosseini-Parvar et al., 2010).
Basil seeds are used as diuretic, antipyretic, antispas-
modic and stomachic (Alcicek et al., 2004). Basil
seeds are black in color and with oval shape and are
good source of ber. When seeds of basil are soaked
in water they swell up and produce gelatinous mass
because poly saccharide layer is present on outer ep-
idermis wall of seed (Azoma and Sakamoto, 2003).
Two major fractions of poly saccharides has been
reported from basil seeds, glucomannan (43%) and
(1-4)-linked xylan (24.29%) and a minor fraction of
glucan (2.31%) (Hosseini Parvar et al., 2010). Suit-
ability of basil seed gum in model system has been
reported recently (Bahram-Parvar and Razavi, 2012).
Recent epidemiological studies have strongly suggest-
ed that consumption of certain plant materials such
as seeds, leaves, fruits and roots may reduce the risk of
chronic diseases related to oxidative stress on account
of their antioxidant activity and promote general
Abstract | Basil seeds are used not only as pharmaceutical plant but also for culinary purpose. e current
study has been undertaken to develop a nutritious, healthy and value added drink. Proximate, mineral anal-
ysis, total polyphenol content and mineral analysis of basil seeds was conducted. Result revealed that basil
seeds are not only good source of ber and protein but they provide appreciable amount of minerals and phe-
nolic compounds. Swollen basil seeds were used to prepare beverage at three supplementation levels i.e. 0.2,
0.3 and 0.4%. Sensory evaluation of basil seed drink revealed that 0.3% basil seeds supplemented drink was
liked most in term of taste, texture and over all acceptability whereas 0.4% basil seeds supplemented drink
were least liked as compared to other treatments. It was concluded that basil seed could be supplemented in
dierent food products for the preparation of value added, healthy and nutritious diets.
Masooma Munir1,2*, Aqsa Qayyum1, Saeeda Raza1, Nouman Rashid Siddique1, Amer Mumtaz1, Naeem
Safdar1, Sahar Shible1, Sohaib Afzal3, Saiqa Bashir4
1Food Science and Product Development Institute, National Agricultural Research Centre, Islamabad, Pakistan; 2Institute of
Food Science and Nutrition, University of Sargodha, Sargodha, Pakistan; 3Institute of Agricultural Sciences, University of Pun-
jab, Pakistan; 4Department of Food science and Technology, University of Poonch, Rawalakot, Pakistan.
Received | June 13, 2016; Accepted | August 16, 2017; Published |----- 00, 2017
*Correspondence | Masooma Munir, National Agricultural Research Centre-Islamabad, Pakistan; Email: masooma_ft@yahoo.com
Citation | Munir, M., A. Qayyum, S. Raza, N.R. Siddique, A. Mumtaz, N. Safdar, S. Shible, S. Afzal and S. Bashir. 2017. Nutritional assessment
of basil seed and its utilization in development of value added beverage. Pakistan Journal of Agricultural Research, 30(3): xxx-xxxx.
DOI | http://dx.doi.org/10.17582/journal.pjar/2017..............
Keywords | Basil seeds, Nutritional plant seed, Poly phenols, Value addition, Beverage
Nutritional Assessment of Basil Seed and its Utilization in Develop-
ment of Value Added Beverage
Utilization basil seed in of in value added beverage
September 2017 | Volume 30 | Issue 3 | Page 2
health benets (Ramarathnam et al., 1995). Phenolic
compounds present in plant parts have been reported
to have multiple biological eects such as antioxidant
activity, antimutagenic, antitumor and antibacterial
properties (Pietta et al. 1998). Essential oil extract-
ed from basil leaves and seeds have antimicrobial and
antioxidant properties. Physical and chemical com-
position of indian and Iranian basil seeds has been
reported by Mathews et al. (1993) and Razavi et al.
(2009). Basil seeds were used as thickening and sta-
bilizing agent because of their high polysaccharide
contents or these were usually processed into essential
oil products in many studies (Rafe et al., 2012) while
information about phenolic prole of basil seeds is
limited. So phenolic content estimation of basil seeds
is necessary to nd relation of basil seed with health
promoting functions, as antioxidant capacity of phe-
nolic compounds protect the cell from free radicals
which cause damage (Zhang et al., 2013).
In recent years, due to busy life styles peoples diet
demand and pattern has been changed. ey tend to
consume delicious, readily available and aordable
foods. Intake of such foods many diseases have been
found in new generation due to low ber intake and
less nutritious components (antioxidant and miner-
als) in ready made food products (NHMRC, 2006).
Food scientists and technologists are doing eorts
to develop nutritious and healthy foods with suita-
ble sensory characteristics. Until now many research
and studies have been conducted on enrichment, sup-
plementation and value addition of food products
such as bakery, dairy and beverages (Alqahtani et al.,
2014; Caleja et al., 2015; Hajmohammadi et al., 2016;
Paquet et al., 2014; Pentikainen et al., 2014). Carbox-
ymethyl cellulose (CMC) is used in most of beverage
as stabilizer and it is obtained from drying the stem
exudates of Asiatic species of alkali-cellulose. is
hydrocolloid is suitable as stabilizers due to its some
properties such as high viscosity at low concentration,
good suspension and low cost (Arinaitwe and Pawlik,
2014).
Beverage is an excellent media to intake nutrients in-
side body, because it is consumed all over the world
to quench thrust and to meet water requirement of
body. is study was designed to assess the nutrition-
al composition of basil seeds and their utilization in
beverages so that nutritional requirement of consum-
ers can be met by economical way. Basil seeds are eas-
ily available and cheaper source of micronutrients.
Materials and Methods
Present research project was conducted at Food Sci-
ence and Product Development Institute, National
Agricultural Research Center, Islamabad. Raw ma-
terials such as basil seeds, sugar, lemons, CMC and
Citric acid were purchased from the local market of
Islamabad. Chemicals for analysis were purchased
from Sigma Aldrich (Seelze, Germany) and Lab-
Scan (Dublin, Ireland) available in the local market.
Chemical analysis of basil seed
Proximate composition such as moisture, ash, crude
fat, crude protein and crude ber of basil seeds was
determined according to the procedures given in
AOAC (2000). For moisture determination samples
were dried in oven at 130ºC for 60 minutes. For ash
determination samples were placed in mued fur-
nace at 550ºC to burn out all carbon compounds
leaving in organic part (ash). Fat was determined by
fat extraction unit by using n. Hexane. For ber deter-
mination, samples were treated with 1.25% Sulphuric
acid and Sodium Hydroxide solution. After ltration
of digested material it was washed with hot water and
then ignited. By calculating loss of weight after igni-
tion, crude ber contents were determined. Protein
contents were determined by using kjeldhal unit.
Total phenolic contents of basil seed
Total phenolic contents were measured by using
Folin–Ciocalteu colorimetric method (Singleton et
al., 1999) spectrophotometrically with some modi-
cations. Extraction of sample (5g basil seeds) was
carried out by using ethanol. One mL of 10% (v/v)
Folin–Ciocalteu reagent in distilled water was mixed
with sample extract. After 6min, 2.0 mL of a20%so-
dium carbonate solution was added. e mixture was
placed at 30 ºC for 60 min. Absorbance was deter-
mined at 765nm. Gallic acid was used as standard and
the total phenol content was expressed as mg GAE/g
of sample.
Mineral analysis of basil seeds
Mineral contents were measured by atomic absorp-
tion spectrophotometric method according to meth-
od given in AOAC (2000). Sample was ashed at 550
ºC in furnace. en digestion of dry ash was carried
out by adding 6M HCL and 0.1 MHNO3 at equal
ratio. After dilution, mineral contents were meas-
ured by using atomic absorption spectrophotometric
method.
Utilization basil seed in of in value added beverage
September 2017 | Volume 30 | Issue 3 | Page 3
Preparation of basil seed beverage
Basil seeds were purchased from local market and
cleaned carefully to remove stones, stalks and dust.
Seed preparation was done by using modied method
based on earlier studies (Razavi et al., 2009). Seeds
were soaked in water at 50ºC for 20 min with water/
seed ratio of 50:1. Frequent stirring was done. en
by using perforated container, swollen seeds were sep-
arated.
For preparation of hydrocolloid solution 2g CMC
powder was dissolved in distilled water at 70ºC for 2
hour with continuous stirring by magnetic hot plate.
1 litter water, 140 g sugar, 2 g salt, 2mL lemon extract,
1g sodium benzoate were mixed together to prepare
beverage for all treatments. Swollen basil seeds were
added according to treatment. is mixture was pas-
teurized and hot lled in 250 mL bottles.
ºBrix and titratable acidity
e pH was determined by a digital pH meter, ºBrix
was measured by Abbe refractometer and Titratable
acidity of the sample was determined according to the
standard method of AOAC (1999).
Sensory evaluation of basil seed beverage
Sensory evaluation of basil seed drinks were evalu-
ated for sensory characteristics such as color, appear-
ance, avor, mouth feel and overall acceptability at
17±5ºC by expert panel of 20 judges (12 females and
08 males) from NARC. e evaluation was done in
sensory evaluation laboratory by a panel with normal
lights on 9-points Hedonic Scale (Land and Shep-
herd, 1988).
Statistical analysis
Results were statistically analyzed by using analysis
of variance technique (ANOVA). e dierence in
means was evaluated by the Least Signicant Design.
is analysis was done by using statistic 9.0 software
(Analytical software, Tallahassee, FL).
Result and Discussion
Proximate composition of basil seeds
Proximate composition of basil seeds in given in Table
2 Basil seeds contain 9.19% moisture, 17.32% crude
protein, 9.68% crude fat, 5.80% ash, % ber and %
carbohydrates.
Results of the present study are comparable with the
work of Razavi et al. (2009) who reported that the
chemical composition of Indian basil seeds was dif-
fered with Iranian basil seeds. ey reported that the
range of moisture content (5-9%), protein (14-20%),
fat (13-23%). ash (5-7%) and carbohydrates (47-
63%) in Indian and Iranian basil seeds. In Iranian
basil seeds protein and fat contents were signicantly
higher while moisture and ash contents were lower
than Indian seeds. Changes in proximate composi-
tion of the present study might be due to change in
soil chemistry, climate condition and dierent agri-
cultural practices (Mathews et al., 1993). Hajmoham-
madi et al. (2016) also determined the chemical com-
position of basil seeds who used swollen basil seeds
for enrichment of fruit based beverage.
Table 1: Treatment plan for 1 liter of beverage
Treatments Raw basil seed (g)
T12
T23
T34
T1= Beverage with 2g basil seeds; T2= Beverage with 3g basil seeds;
T3= Beverage with 4g basil seeds
Table 2: Proximate composition of Basil seeds (% wet
basis)
Moisture 9.19±0.45
Protein 17.32±1.12
Fat 9.68±0.67
Fiber 7.11±0.39
Ash 5.80±0.11
Carbohydrates 50.9±0.35
Table 3: Total phenols(mg GAE/g) and mineral con-
tents (ppm)
Total phenols 63.78±1.75
Fe 22.74±1.01
Zn 15.81±0.93
Mg 315.53±2.15
Mn 10.11±0.87
Total phenol and mineral contents of basil seeds
Total phenolic contents in basil seeds are 63.78±1.75
mg GAE/g as shown in Table 3. Standard calibra-
tion curve for total phenols has also been shown in
Figure 1. Our ndings are dierent from previous
ndings of Shen et al. (2015), which might be due
to change in soil chemistry. Mineral contents of food
have nutritional importance as far as body physiology
Utilization basil seed in of in value added beverage
September 2017 | Volume 30 | Issue 3 | Page 4
concerned. Deciency of micronutrient is prevailing
around the world. High Mg contents may boost bone
development and strength also eective against heart
stroke. Zinc has also been linked with alleviating hy-
perglycemia in diabetics. Fe is important as part of
blood (Fallon and Enig, 2001).
Mineral contents in basil seeds have been reported
in Table 3. Basil seeds and leaves are good source of
minerals. Mg contents were found in high amount
(315.53±2.15) as compared to other determined min-
erals. Agunbiade et al. (2015) has reported mineral
contents in basil seeds and leaves. Mlitan et al. (2014)
also studied mineral contents of basil from Libya but
change in mineral concentration was observed be-
tween our and their ndings which might be due to
varietal dierences and soil chemistry.
ºBrix and acidity of basil seed drink
Titratable acidity and ºBrix of basil seed drinks have
been shown in Table 4. ere was no signicant
change in ºBrix and acidity of beverage. ese results
are in agreement with other previous studies (Jabbar
et al., 2014; Saeeduddin et al., 2015).
Table 4: ºBrix and Acidity of beverage
Treatment (T) ºBrix Acidity
T112.9±0.09 0.168±0.01
T212.7±0.05 0.160±0.03
T312.5±0.07 0.155±0.01
T1= Beverage with 2g basil seeds; T2= Beverage with 3g basil seeds;
T3= Beverage with 4g basil seeds
Figure 1: .........?
Sensory evaluation of basil seed beverage
Eect of seed ratio on sensory parameters (color, a-
vor, taste, texture and overall acceptability) of drink is
presented in Figure 2. All treatment were in accept-
able range but T2 secured high sensory scores in term
of color (7.63± 0.29), avor (7.43±0.41) taste (7.50±
0.32) texture (8.01± 0.45) and overall acceptability
(8.11±0.37) followed by T1 and T3. Reduction in de-
sirability of taste avor and texture with increase in
seed ratio was also reported by Hajmohammadi et al.
(2016). It might be due to decrease in total soluble
solids of drink because seeds had previously swollen
in water and had amounts of water inside and be-
tween them.
Figure 1: .........?
Conclusion
Our ndings revealed that basil seeds are good source
of micronutrients and have antioxidant potential
which is highly benecial for human health. Swol-
len basil seeds in beverage also showed good sensory
scores (T2) in beverage which shows it would be ac-
ceptable by consumers too. erefore, further studies
should be conducted about polyphenol composition
and vitamins to better understanding of nutritional
importance of basil seed.
Authors’ Contributions
Masooma Munir and Aqsa Qayyum: Executed re-
search plan. Nouman Siddique
Amer Mumtaz: ey have participated in research
analysis
Naeem Safdar: wrote abstract
Saeeda Raza: reviewed the manuscript.
Sahar Shibbli and Saiqa Bashir: Helped in drafting
the manuscript
Sohaib Afzal: Statistical analysis
Acknowledgement
e present study was supported by the Food Science
and Product Development Institute, National Agri-
Utilization basil seed in of in value added beverage
September 2017 | Volume 30 | Issue 3 | Page 5
cultural Research Center (NARC), Pakistan.
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