ArticlePDF Available



Abstract and Figures

Objective: The objective of the study was to evaluate the in-vivo anti-hyperlipidemic activity of Capsicum frutescens extracts.Methods: The dried fruit powder were extracted with a three liquid phase extraction system. The acetone extract was isolated and the anti-hyperlipidemic activity was evaluated.Results: The anti-hyperlipidemic study was carried out by inducing hyperlipidemia in rats by means of triton. The serum collected was analyzed for total cholesterol, triglyceride, low-density lipoprotein and high-density lipoprotein.Conclusion: The result of the present study revealed that the acetone extract of the fruits of Capsicum frutescens possess anti-hyperlipidemic activity.
Content may be subject to copyright.
Original Article
Research Scholar, Bhagwant University, Ajmer, Rajasthan, India,
Department of Pharmacology, Shree Devi College of Pharmacy,
Mangalore, 574 142, Karnataka, India
Received: 04 Jan 2017, Revised and Accepted: 31 Apr 2017
Objective: The objective of the study was to evaluate the in-vivo anti-hyperlipidemic activity of Capsicum frutescens extracts.
Methods: The dried fruit powder were extracted with a three liquid phase extraction system. The acetone extract was isolated and the anti-
hyperlipidemic activity was evaluated.
Results: The anti-hyperlipidemic study was carried out by inducing hyperlipidemia in rats by means of triton. The serum collected was analyzed for
total cholesterol, triglyceride, low-density lipoprotein and high-density lipoprotein.
Conclusion: The result of the present study revealed that the acetone extract of the fruits of Capsicum frutescens possess anti-hyperlipidemic activity.
Keywords: Capsicum frutescens, Antihyperlipidemic activity, HDL-LDL, Triton
© 2017 The Authors. Published by Innovare Academic Sciences Pvt Ltd. This is an open access article under the CC BY license (
DOI: 9i4.21289
Hyperlipidemia, also known as hyperlipoproteinemia or
dyslipidemia, is an abnormal elevation of lipid levels in the
bloodstream. These lipids include cholesterol, cholesterol
compounds, phospholipids and triglycerides, all carried in blood as
large molecules called lipoproteins. There are three types of
hyperlipidemia namely hyperlipoproteinemia (elevated levels of
lipoprotein in the blood), hypercholesterolemia (high cholesterol)
and hypertriglyceridemia (high triglycerides level in blood).
Hyperlipidemia affects lipid production, transportation in the
bloodstream and/or deposition in body cell. It is the first and foremost
factor that leads to diseases like atherosclerosis, coronary heart
disease, ischemic cerebrovascular disease, hypertension, obesity and
diabetes mellitus (Type-II) etc. [1, 2].
Although human beings have developed many allopathic drugs to
combat the hyperlipidemia and these drugs to a certain extent have
shown promising lipid-lowering activity. But these drugs have been
found to be associated with side effects. As a result of side effects
that are mostly associated with the allopathic system of medicine,
the world population today is turning back to a traditional system of
medicine as these medicines are free from side effects, easily
available with less cost. Thus, in the present scenario, more research
activities are required for the development of the drug from the
natural sources to meet the demand of world population.
Capsicum frutescens is a vegetable used daily, and the substance
capsaicin is responsible for its hot and spicy flavour, sought after in
gastronomy. Capsaicin and several related molecules are known by
the collective name capsaicinoids, and they are produced by all
plants of the genus Capsicum, with the exception of the bell pepper
(Capsicum annum), which produces no capsaicin.
The naturally occurring content of capsaicinoids in spices ranges
typically from 0.1 mg/g in chilli pepper to 2.5 mg/g in red pepper
and 60 mg/g in oleoresin red pepper [3]. Capsaicin and
dihydrocapsaicin are the major capsaicinoids produced; however,
others exist and are produced in smaller quantities.
Capsaicin is also known to inhibit Substance P, a neuropeptide that
is the key transmitter of pain to the brain thus providing relief to
pain. It has potent antibacterial properties that fight and prevent
chronic sinus infections, or sinusitis. Capsaicin is also a thermogenic
agent, which means it increases metabolic activity. This, in turn,
helps to burn calories and fat. Capsaicin may help to protect the
heart by reducing cholesterol, triglycerides and platelet aggregation.
Capsaicin is claimed to have high antioxidant values and HDL-
cholesterol-raising effect. Hence this study aims at exploring the
possible effects of Capsaicin on serum TC, TG, LDL and HDL levels in
experimental rats.
Triton WR 1339 (Sigma-Aldrich), Cholesterol (SRL Mumbai),
Atorvastatin (Micro labs Pvt Ltd, B, lore), Anaesthetic ether-SD Fine-
chem Ltd., Mumbai, Chloroform-SD Fine Chem Ltd. Mumbai.
Formaline-SD Fine-chem Ltd., Mumbai. All chemicals and reagents
were of analytical grade. Diagnostic kits used for estimation of
cholesterol, triglycerides, HDL, LDL, VLDL were procured from
Robonik Diagnostic Ltd India.
Male Wistar rats aged between 8-10 w (250-300 g) were used for
the study. Animals were kept in the controlled condition in the
institutional animal house at an ambient temperature of 25-30 °C
and relative humidity of 55-60% and 12/12 h light/dark cycle and
were provided pellet diet al. ong with water ad libitum. The
experimental protocol was accepted by Institutional Animal Ethics
Preparation of fruit extract
Mature Capsicum frutescens (bird’s eye chilli) were collected and
washed thoroughly with water and air dried in shade at room
temperature. It was ground into powder by a miniature high-speed
universal pulveriser. The three liquid phase extraction system of
acetone, K2HPO4 and n-hexane was prepared by weighing acetone
22% (w/w), K2HPO4 20% (w/w), n-hexane 10% (w/w) and water
(58% (w/w) and mixing. The powdered Capsicum frutescens was
added to this three liquid phase extraction system in a mass ratio of
1:20. The mixture was thoroughly vibrated for 10 min and then set-
tled at room temperature. After the separation of three phases, the
volume of the acetone was removed from the middle layer by using a
pipette and kept aside. This procedure was done again for three
International Journal of Current Pharmaceutical Research
ISSN- 0975-7066 Vol 9, Issue 4, 2017
Thomas et al.
Int J Curr Pharm Res, Vol 9, Issue 3, 165-168
times by adding fresh acetone into the same extract. The acetone
extracts were pooled and dried under vacuum. The percent yield of
capsaicin was 10 mg/g of Capsicum frutescens powder.
Electrospray mass spectrometry analysis
The electrospray mass spectrometric scans were monitored to check
for the presence of Capsaicinoids using AB Sciex 4000 mass
spectrometer with direct injection of sample dissolved in Methanol
in MS scan mode into the mass detector.
Dose preparation of standard and leaves extracts
Atorvastatin at a dose of 10 mg/Kg was prepared by suspending in
5% sucrose in water. The extract of leaves was dissolved in 5%
sucrose in water. Doses of 50 mg/Kg b.w. and 100 mg/Kg b.w. of the
extract were administered by oral route to the rats.
In vivo acute oral toxicity studies
In the present study, the acute oral toxicity of the extracts was
performed according to OECD Guideline 423 [4]. In this method, the
toxicity of the extracts was evaluated using the stepwise procedure,
each step using three Wistar rats. The rats were fasted prior to dosing
(food but not water should be withheld) for three to four hrs.
Following the period of fasting the animals were weighed and the
extract was administered orally at a dose of 1000 mg/Kg b.w. Animals
were observed individually after dosing at least once during the first
30 min; periodically during the first 24 h with special attention given
during the first 4 h and daily thereafter, for a total of 14 d.
Experimental design
A total of 30 male wistar rats were utilized and the animals were
randomly divided into 5 groups of 6 rats in each group:
Group I-Normal [standard diet+5% sucrose (p. o.)]
Group II–Diabetic control [standard diet+triton (i. p.)+5% sucrose
(p. o.)]
Group-III-Standard drug (10 mg/Kg b.w., p. o.)+triton+standard diet.
Group-IV-Diabetic control+Capsicum frutescens extract (50 mg/kg
body weight which is 5% of the dose used for acute toxicity
studies)+triton+standard diet
Group–V-Diabetic control+Capsicum frutescens extract (100 mg/kg
body weight which is 10% of the dose used for acute toxicity
studies)+triton+standard diet
Effect in Triton-induced hyperlipidemic rats
Animals were kept for fasting for 24 h and was injected with a
solution of Triton WR-1339 (dissolved in 0.9% NaCl) at a dose of
400 mg/kg body weight intraperitoneally. The plant extracts, at a
dose of 50 mg/kg and 100 mg/kg body weight were administered
orally through gastric intubation. The first dose was given
immediately after triton injection and a second dose 20 h later.
Blood samples were collected at 6, 24 and 48h after triton injection
and were used for the study of various biochemical parameters.
Blood was collected through retro-orbital plexus route on anesthesia
and centrifuged at 2000 rpm for 30 minutes [5-6].
Biochemical analysis
Serum samples were analyzed for
Total serum cholesterol (TC),
Triglyceride (TG)
High-density lipoprotein cholesterol (HDL-C)
Low-density lipoprotein (LDL-C)
LDL-C/HDL-C ratio was calculated as the ratio of plasma LDL-C to
HDL-C levels.
Data analysis
Data were statistically analyzed as mean+SEM and expressed as
non-significant P>0.05, just significant P<0.05, significant P<0.01
and highly significant P<0.001 by using ANOVA followed by
Dunnett’s t-test and unpaired t-test with Welch correction.
Electrospray mass spectrometry analysis
The presence of intense peaks at 306.3 and 308.3 indicated the presence
of the Capsaicinoids–Capsaicin and Dihydrocapsaicin (fig. 1).
Fig. 1: MS scan showing the peaks for capsaicin and dihydrocapsaicin
In vivo acute oral toxicity studies
Acute oral toxicity studies for the extracts of the leaves of Capsicum
frutescens revealed that both the extracts were non-toxic attested
dose levels and well tolerated by the experimental animals.
Effect on the lipid profile
The results of the lipid profile are tabulated in tables 1-3. The extract
showed a decrease in blood lipids in hyperlipidemic rats when
compared with the respective diabetic controls. The extract showed
Thomas et al.
Int J Curr Pharm Res, Vol 9, Issue 3, 165-168
a significant decrease in TC, TG and LDL along with an increase in
HDL level dose-dependently at the tested doses (50 mg/kg and 100
mg/kg) when administered in Triton-induced hyperlipidemic rats
(tables 1-3).
Table 1: Effect of capsaicinoids on total cholesterol and triglyceride levels in triton induced hyperlipidemic rats (Values are mean+SEM of
6 animals in each group)
Cholesterol (mg/dl)
Triglycerides (mg/dl)
Diabetic control
0 mg
0 mg
P>0.05 (Dunnett’s t-test)
P<0.001 (Unpaired t-test)
Table 2: Effect of capsaicinoids on LDL and HDL levels in triton induced hyperlipidemic rats (Values are mean+SEM of 6 animals in each
HDL (mg/dl)
LDL (mg/dl)
Diabetic Control
0 mg
0 mg
P>0.05 (Dunnett’s t-test)
P<0.001 (Unpaired t-test)
Table 3: Effect of capsaicinoids on TC/HDL ratio and LDL/HDL ratio in triton induced hyperlipidemic rats
TC/HDL ratio
LDL/HDL ratio
Diabetic Control
0 mg
0 mg
The three liquid phase system consisting of n-hexane, acetone,
K2HPO4, and water was successfully used to extract capsanthin and
capsaicin. Capsanthin and capsaicin were partitioned mainly into the
n-hexane and acetone phases, respectively. They could be easily
obtained after removing the solvent for further purification. TLPE
was an effective method for the extraction and separation of
capsaicinoids [7].
Triton WR 1339, when administered in fasted rats, causes an
elevation in plasma lipid level. Initially there is a sharp increase in
the lipid level reaching a peak two to three times the control value
by 24h after the administration of Triton injection (Phase I–
synthetic phase), this increase in lipids will fall off within the next
24h i.e. 48h after the administration of Triton (Phase II–Excretion
phase) This increase in plasma lipids by Triton is thought to be due
to one of the following mechanisms; as due to increase in hepatic
synthesis of cholesterol or Triton physically alters very low density
lipoproteins (VLDL) rendering their removal from the blood. Drugs
interfering with cholesterol synthesis were shown to be active in
phase I, while drugs interfering with cholesterol excretion and
metabolism was active in Phase II [8-10].
Total cholesterol (TC) was determined by one step method of
Wybenga and Pillegi [7] based on the reaction between cholesterol
and cholesterol reagent (ferric oxide, ethyl acetate and sulfuric acid).
High-density lipoprotein (HDL) from blood was determined by a
two-step method i.e. initial separation of HDL from blood using a
precipitating agent and then the precipitated HDL was determined
by using calorimetric reaction with cholesterol reagent [11].
Triglyceride (TG) was determined calorimetrically by an enzymatic
reaction using glycerol-3-phosphate oxidase. Enzymatic splitting of
lipoprotein lipase along with reaction between 4-aminoantipyrin, 4-
chlorophenol and H
under the catalytic action of peroxidase
generates quinimine which is used as an internal indicator in this
calorimetric determination [12]. LDL was determined by using
Grindelwald's formula–LDL = TC-HDL-(TG/5) [13].
The preliminary screening revealed the presence of Capsaicin and
dihydrocapsaicin in the acetone extract. Capsaicinoids significantly
decreased the plasma triglycerides, total cholesterol, LDL
cholesterol and increased the high-density lipoprotein cholesterol.
The cholesterol-lowering action of capsaicinoids was attributed to
the inhibition of hepatic cholesterol synthesis, also by the
stimulation of the conversion of cholesterol to bile acids by up-
regulation of cholesterol 7α-hydroxylase expression and increasing
the excretions of bile acids in feces [14].
In conclusion, the present study shows that Capsaicinoids extracted
from Capsicum frutescens has a plasma LDL-C, cholesterol,
triglyceride lowering effect and has a plasma HDL-C raising effect.
We think that it is interesting and meaningful to report that dietary
intake of Capsaicinoids can improve plasma lipid levels.
L. T. designed all the experiments, carried out the main experimental
work and wrote the manuscript. J. V. K supported the idea of the
manuscript and provided a number of suggestions.
Declare none
Thomas et al.
Int J Curr Pharm Res, Vol 9, Issue 3, 165-168
1. Carrero JJ, Baro L, Fonolla J, Santiago MC, Ferez AM, Castillo R,
et al. Cardiovascular effects of milk enriched with x-3
polyunsaturated fatty acids, oleic acid, folic acid and vitamins E
and B6 in volunteers with mild hyperlipidemic. Nutrition
2. Sies H, Stahl W, Sevanin A. Nutritional dietary and postprandial
oxidative stress. J Nutr 2005;135:969-72.
3. Parrish M. Liquid chromatographic method for determining
capsaicinoids in capsicums and their extractives: collaborative
study. J AOAC Int 1996;79:738-45.
4. Guideline OT. 423. Acute Oral Toxicity–Acute Toxic Class
Method, adopted; 2001. P. 17.
5. Sikarwar MS, Patil MB. The anti-hyperlipidemic activity of
crataeva nurvala buch-hum ethanolic extract fractions. Int Med
J Sifa University 2015;2:31-6.
6. Jadeja RN, Thounaojam MC, Patel V, Devkar RV, Ramachandran
AV. Antihyperlipidemic potential of a polyherbal preparation
on triton WR 1339 (Tyloxapol) induced hyperlipidemia: a
comparison with lovastatin. Int J Green Pharm 2009;3:119-24.
7. Yan-Yan Dang, Hua Zhang, Zhi-Long Xiu. Three-liquid-phase
extraction and separation of capsanthin and capsaicin from
capsicum annum L. Czech J Food Sci 2014;32:109-14.
8. Moss JN, Dajani EZ. Antihyperlipidemic agents in Screening
methods in pharmacology, edited by Turner RA, Hebben PA.
Academic Press: New York; 1971. p. 121.
9. Wybenga DR, Pileggi VJ, Dirstine PH, Di Giorgio J. Direct manual
determination of serum total cholesterol with a single stable
reagent. Clin Chem 1970;16:980.
10. Devi R, Sharma DK. Hypolipidemic effect of different extracts of
Clerodendron colebrookinum Walp in normal and high-fat diet
fed rats. J Ethnopharmacol 2004;90:63.
11. Demacker PNM, Vos-Jansees HE, Jansen AP, Von’t Laar A.
Evaluation of the dual precipitation method by comparison
with the ultracentrifugation method for measurement of
lipoproteins in serum. Clin Chem 1977;23:1238.
12. Cole TG, Klotzch SG, Mc Namara J. Measurement of TG
concentration, in Handbook of lipoprotein testing, edited by
Rifai N, Warnick GR, Domnizeck MH. Washington, AACC Press;
1997. p. 115.
13. Friedelwald WT, Levy RI, Friedrickson DS. Estimation of the
concentration of LDL cholesterol in plasma without
preparation or ultracentrifugation. Clin Chem 1972;18:449.
14. Zhang L, Zhou M, Fang G, Tang Y, Chen Z, Liu X.
Hypocholesterolemic effect of capsaicinoids by increased bile
acids excretion in ovariectomized rats. Mol Nutr Food Res
How to cite this article
Licto Thomas, Jagadish Vasudev Kamath. Evaluation of the
antihyperlipidemic activity of Capsicum frutescens extract. Int J
Curr Pharm Res 2017;9(4):165-168.
... Capsaicinoids, Capsaicin stimulates the conversion of cholesterol to bile acids by regulation of cholesterol 7α-hydroxylase expression and enhancing the excretions of bile acids in feces [28] A randomized, double-blind, controlled clinical trial [29] 19. Neem ...
Full-text available
Hyperlipidemia is a popular disorder and a state of body where there is elevated level of triglyceride above 200mg/dl, LDL above 160mg/dl, Total cholesterol above 200mg/dl and HDL below40mg/dl. Now it can be found on every other person, when LDL increases it travels through our blood vessels and tends to accumulate in the vessels the accumulation is not for lipids only it consists of calcium and fibrous plaque, and this scenario leads to atherosclerosis. Atherosclerosis causes narrowing of blood vessels, leads to lesser blood flow to the heart results in angina pectoris and gradually heart attack. There are many marketed medicines fighting against this, but they have adverse effects like muscle toxicity. Here, are some natural remedies which we come across everyday are fighting these situations which are very easily available in nature, and we can take it in our regular diet.
... Hyperlipidemia is also caused by a decreased level of high density lipoprotein in blood. Hyperlipidemia with an elevated level of lipoproteins is measured by the initiation and progression of plaque formation in arteries which may causes thrombosis and myocardial infraction [3]. Control and reduction the lipid level is necessary for freedom from coronary artery diseases. ...
Full-text available
Introduction and objective Hyperlipidemia is considered as a serious communal problem in developed countries, caused by an excess level of cholesterol in blood circulation. It leads to chronic illness and even death in human beings. As the currently available drugs cause unexpected side-effect, the aim of this study is to concentrate on naturally occurring flavonoids which can potentially provide defensive and therapeutic effects in atherosclerosis diseases, and investigate the hypolipidemic effect of rutin on Triton WR-1339 triggered hyperlipidemia in a rat blood sample. Material and methods Rats were randomly prearranged into five different groups of five rats each. Group-I was the non-disease control and administered normal saline. Group-II was the atherogenic control, administered Triton WR 1339 (200 mg/kg BW). Group-III was standard and received Atorvastatin. The last two groups (IV, V) were tested (I&II) by administering administered Rutin (40 mg/kg, 80 mg/kg) orally. The test material (I&II) and the standard drug were administered for seven days. After the last dose, blood samples were collected and the lipid levels estimated in the blood samples. Results Rats treated with rutin flavonoid at a dose of 40 mg/kg & 80mg/kg exhibited a reduction in Total Cholesterol, Triglycerides, Low Density Lipoprotein (LDL) and Very Low Density Lipoprotein (VLDL). Rutin also increases the High Density Lipoprotein (HDL), compared with control rats. Rutin treated rats exhibited dose-dependent hypolipidemic activity. The protection percentage of rutin against hyperlipidemia was observed as 41.89%, 55.57% whereas the atorvastatin treated group protection was observed at 60.63%. Conclusions The results of the study revealed that rutin showed a significant hypolipidemic effectiveness on Triton WR-1339 induced hyperlipidemia in rats.
Full-text available
The extraction of capsanthin and capsaicin from red pepper (Capsicum annum L.) was studied using a three-liquid-phase system (TLPS) of acetone/K2HPO4/n- hexane. When the system consisted of 22% (w/w) acetone/20% (w/w) K2HPO4/10% (w/w) n-hexane, capsanthin was extracted into the top n-hexane-rich phase, yielding a recovery of 98.15% at a temperature of 25°C. Meanwhile, capsaicin was mainly distributed in the middle acetone-rich phase, less than 0.01% in the top phase, and undetectable in the bottom salt-rich phase. The yields of capsanthin and capsaicin were 105 and 88% of those of the conventional solvent extraction, respectively. Thus, capsanthin and capsaicin were separated through a single step at a low cost.
Full-text available
Aim: This study investigates the anti-hyperlipidemic effect of Crataeva nurvala Buch-Hum ethanolic extract fractions in triton and atherogenic diet-induced hyperlipidemic rats. Methods: Oral administrations of 500 mg/kg body weight of various fractions of selected plant were evaluated for possible hyperlipidemic activity in triton and atherogenic diet-induced hyperlipidemic rats for duration of 48 hours and 14 days, respectively. A comparative assessment was also made between the actions of selected drug with known anti-hyperlipidemic drug simvastatin. The outcomes of the study were expressed as mean± standard error (SE) and data was evaluated by using analysis of variance (ANOVA), followed by Dunnett’s t-test for multiple comparisons. Results and Discussion: Ethanolic extract Ethyl acetate fraction of Crataeva nurvala stem bark 500 mg/kg body weight on oral administration exhibited a significant reduction (P < 0.01) in serum lipid parameters like triglycerides, total cholesterol, low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), and increase in high-density lipoprotein (HDL) in hyperlipidemic rats as compared with hyperlipidemic control in both models. Conclusion: Our results demonstrated that Crataeva nurvala Buch-Hum ethanol extract ethyl acetate fractions possessed significant anti-hyperlipidemic activity.
Sixteen laboratories participated in an AOAC- American Spice Trade Association (ASTA) collaborative study of a liquid Chromatographic (LC) method for determining capsaicinoids in capsicums and their extractives. Capsicums are extracted with ethanol by refluxing and then filtered. Capsaicinoids in the filtrate are separated by reversed-phase LC and detected spectrophotometrically with external standard quantitation. Participants analyzed 6 ground capsicum and 3 oleoresin products as 12 samples from a mixed scheme of blind duplicates and Youden matched pairs. Average repeatability and reproducibility standard deviations (sr and SR, respectively) and average relative standard deviations (RSDr and RSDR, respectively) for ground red pepper were as follows: sr, 610 Scoville heat units (SHU); SR, 1730 SHU; RSDr, 1.7%; RSDR, 4.9%. For ground chili peppers, the values were sr, 60 SHU; SR, 160 SHU; RSDr, 4.0%; RSDR, 10.6%. For oleoresin red pepper, the averages were sr, 46820 SHU; SR, 54990 SHU; RSDr, 8.5%; RSDR, 11.2%. The LC method has been adopted first action by AOAC INTERNATIONAL.
Scope: This study investigated the interaction of dietary capsaicinoids with the mRNA and protein expressions of key receptors and enzymes involved in cholesterol metabolism in ovariectomized (OVX) rats. Methods and results: Female Sprague-Dawley rats were subjected to sham operation or ovariectomy. The sham group and OVX control group were fed with high-cholesterol diets, whereas the treatment group (control diet containing 0.01% capsaicinoids) was fed with high-cholesterol plus 0.01% capsaicinoids diet for 21 days. Capsaicinoids significantly decreased the body weight gain, plasma total cholesterol, LDL cholesterol, and triacylglycerol without affecting the high-density lipoprotein cholesterol in the OVX rats. The change in plasma lipoprotein profile was accompanied by a greater excretion of total bile acid in feces and small intestinal contents. Western blot and real-time PCR analyses revealed that capsaicinoids significantly enhanced the expressions of hepatic cholesterol 7α-hydroxylase and transient receptor potential vanilloid type-1 but did not affect the expression of 3-hydroxy-3-methylglutaryl-CoA reductase in the OVX rats. Conclusion: Capsaicinoids have cholesterol-lowering effects in OVX rats. The hypocholesterolemic activity of capsaicinoids is caused by the stimulating conversion of cholesterol to bile acids by upregulation of cholesterol 7α-hydroxylase expression and the increase in fecal total bile acid excretion.
We investigated the antihyperlipidemic activity of an aqueous extract of a herbal preparation from a combination of six Indian medicinal plants. The current study was undertaken to assess the hypolipidemic, hypocholesterolemic and hypotriglyceridemic potential of the polyherbal extract using Triton WR 1339 (Tyloxapol) induced hyperlipidemia. The animals were divided into four groups′normal control, hyperlipidemic control, hyperlipidemic plus polyherbal extract and hyperlipidemic plus Lovastatin. Hyperlipidemia was induced by single intravenous injection of Triton WR 1339. Intragastric administration of polyherbal extract (500mg/kg of body weight) significantly decreased plasma cholesterol, triglyceride, non-HDL-C and phospholipids levels and increased HDL-C levels. Atherogenic index and triglyceride secretion rate were lowered in the polyherbal extract fed animals when compared to hyperlipidemic animals. Polyherbal extract exhibited quite competitive potential when compared with the reference drug Lovastatin affording a possible alternative therapeutic agent in the treatment of hyperlipidemia.
We evaluated the dual-precipitation method for quantitative measurement of lipoproteins as described by Wilson and Spiger [J. Lab. Clin. Med. 82, 473 (1973)] for normo- and hyperlipemic sera, by comparison with the results obtained with ultracentrifugation. If serum with an above-normal triglyceride concentration is analyzed, the very-low-density lipoprotein cholesterol value obtained with the precipitation method is usually too low. For measurement of high-density lipoprotein cholesterol the ultracentrifugation and precipitation procedures give comparable results, but the latter method is preferred because sinking pre-beta-lipoproteins present in the high-density lipoprotein fraction isolated by means of the ultracentrifuge may result in falsely high values for cholesterol in that fraction. Therefore, at least for the determination of very-low-density lipoprotein cholesterol in hyperlipemic serum, the use of an ultracentrifuge remains necessary. Because few laboratories have an ultracentrifuge at their disposal, it seemed important to look at the stability of sera in view of the forwarding of samples. Also, a way of increasing the efficiency of the ultracentrifuge was studied. Sera can be stored for a week at 4 degrees C or for 54 h at room temperature without noticeable effect on lipoprotein values. Moreover, reliable values can be obtained with an ultracentrifugation time of 8 h (0.8 X 10(8) g-min).