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EVALUATION OF FOOT CREAMS FORMULATION ON HUMAN SKIN – A NOVEL APPROACH

Article

EVALUATION OF FOOT CREAMS FORMULATION ON HUMAN SKIN – A NOVEL APPROACH

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The traditional Indian medicine-Ayurveda, describes various herbs, fats, oils and minerals with anti-aging as well as wound healing properties. With aging, numerous changes occur in skin, including decrease in tissue cell regeneration, decrease in collagen content, loss of skin elasticity and mechanical strength. We prepared five topical anti-aging formulations using cow ghee, flax seed oil, Phyllanthus emblica fruits, Shorea robusta resin, Yashada bhasma as study materials. For preliminary efficacy evaluation of the anti-aging activity we chose excision and incision wound healing animal models and studied the parameters including wound contraction, collagen content and skin breaking strength which in turn is indicative of the tissue cell regeneration capacity, collagenation capacity and mechanical strength of skin. The group treated with the formulations containing Yashada bhasma along with Shorea robusta resin and flax seed oil showed significantly better wound contraction (P < .01), higher collagen content (P < .05) and better skin breaking strength (P < .01) as compared to control group; thus proposing them to be effective prospective anti-aging formulations.
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Wound healing occurs as a fundamental response to tissue injury. Several natural products have been shown to accelerate the healing process. The present investigation was undertaken to determine the efficacy of topical administration of an alcoholic bark extract of Butea monosperma (B. monosperma) on cutaneous wound healing in rats. Full-thickness excision wounds were made on the back of rat and B. monosperma extract was administered topically. The granulation tissue formed on days 4, 8, 12 and 16 (post-wound) was used to estimate total collagen, hexosamine, protein, DNA and uronic acid. The extract increased cellular proliferation and collagen synthesis at the wound site, as evidenced by increase in DNA, total protein and total collagen content of granulation tissues. The extract treated wounds were found to heal much faster as indicated by improved rates of epithelialization and wound contraction, also confirmed by histopathological examinations. Also, the tensile strength of drug-treated wounds was increased significantly. In addition, we show that B. monosperma possesses antioxidant properties, by its ability to reduce lipid peroxidation. The results clearly substantiate the beneficial effects of the topical application of B. monosperma in the acceleration of wound healing.
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The penetration depth of light in diffuse reflectance near-infrared spectroscopy for measuring water content in skin is assessed both from theoretical and experimental points of view. The Monte Carlo simulation was implemented to investigate the dependencies of the light penetration depth on a source-detector distance. To compare with the simulation results, an in vivo experiment for water contents of skin was performed introducing two different optical fiber probes. It is found that the minimum separation between a source and detector fibers influences largely the measurement depth. The larger separation leads to a deeper measurement depth at a particular wavelength. The measurement depth is also influenced fairly by the absorption coefficient of the tissue. The larger absorption coefficient results in a shallower measurement depth. The correlations between the water contents measured by the optical and capacitance techniques were discussed. The dependencies of the light penetration depth on the source-detector geometry and wavelength are presented.
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The water content of skin was determined using a FT near infrared (NIR) spectrometer. NIR diffuse reflectance spectra were collected from hairless mouse, in vitro, and from human inner arm, in vivo. It was found that the variation of NIR absorbance band 1450 nm from OH vibration of water and 1940 nm from the combination involving OH stretching and OH deformation, depending on the absolute water content of separated hairless mouse skin, in vitro, using the FT NIR spectrometer. Partial least squares regression (PLSR) was applied to develop a calibration model. The PLS model showed good correlation. For practical use of the evaluation of human skin moisture, the PLS model for human skin moisture was developed in vivo on the basis of the relative water content of stratum corneum from the conventional capacitance method. The PLS model predicted human skin moisture with a standard errors of prediction (SEP) of 3.98 at 1130-1830 nm range. These studies showed the possibility of a rapid and nondestructive skin moisture measurement using FT NIR spectrometer.
The moisturize influence of the commercial cosmetics on the foot skin
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  • J Pavlackova
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Polaskova J., Pavlackova J., Vltavska P., Janirkova G., Kasparkova V. and Janis R. The moisturize influence of the commercial cosmetics on the foot skin. Advances in Environment, Biotechnology and Biomedicine, 2012; 127: 272-277.
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Wound healing and anti microbial activity of formulated cream of leaf volatile oil of Atalintia monophylla Correa
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Manimaran S, Loganathan V, Akilandeswari S, Jaswanth, A, Sathya, S and Ruckmani K. Wound healing and anti microbial activity of formulated cream of leaf volatile oil of Atalintia monophylla Correa. Haddard Medicus, 2002; 45: 59-62.