Fatigue specimens of A508-3 steel were irradiated in the swimming-pool test reactor in China Institute of Atomic Energy, the fluence was 3×10¹⁹ n/cm² at 300°C, then low-cycle fatigue tests were carried out at ambient temperature, with the fatigue strain range is 0.32-1.8%. The results indicate that, irradiated A508-3 specimens exhibit cyclic softening and instability behavior during the test, and the cyclic softening rate increased with strain range increased; fatigue life decreased from 1.7×10⁵ to about 5×10², as the strain range increased from 0.32% to 1.8%, the fatigue life of A508-3 steel increased after the neutron irradiation; fatigue fracture initiated at the surface of specimen, and more individual cracks formed on the specimens of higher strain range compared with the specimens of lower strain range.