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231
Proc. Fla. State Hort. Soc. 129: 2016.
Optimal Photoperiod Treatment for Flowering of
Bougainvillea xbuttiana ‘Afterglow’
Mun Wye Chng* and KiMberly a. Moore
University of Florida, IFAS, Fort Lauderdale Research and Education Center
3205 College Avenue, Davie, FL 33314
AdditionAl index words. photoperiod, owering
Bougainvillea xbuttiana cultivars are mostly considered qualitative short-day plants that ower more readily and pro-
fusely under short day lengths. The cultivar ‘Afterglow’ was tested in two experiments to conrm that it is a qualitative
short-day plant, and to discover the optimal photoperiod under which owering is most profuse. Seventy plants were
subjected to a range of photoperiods from 8–14 hours in 1-hour increments, to establish an optimal photoperiod for
the induction of owering in the absence of other environmental factors, namely water stress, physical wounding, or
high nutrient availability. Experiment 1 conrmed that ‘Afterglow’ is a short-day plant, producing the most owers
between 8 and 11 hours of daylight. Plants under long-day or short-day with night interruption provided by a single
white LED bulb producing 240 μmol·m-2·s-1 of photosynthetic photon ux did not produce owers. Experiment 2 showed
that plants grown under an 8-hour photoperiod produced the largest number of owers.
*Corresponding author; email: mwchng@u.edu
Scientic studies related to the owering of horticultural plants
in tropical and subtropical zones of the world, including the United
States, have largely focused on the economically important tropi-
cal fruit crops of several key genera, namely Mangifera (mango),
Citrus (citrus) and Litchi (lychee) (Koshita and Takahara, 2004;
Menzel, 1983; Núñez-Elisea and Davenport, 1994; Ramírez
and Davenport, 2010). Less attention has been paid to woody
ornamentals in recent years, as the owering behaviors of most
landscape plants are already well understood.
Ornamental crops in the tropics can generally be classied as
foliage crops and oral crops. The difference between tropical/
sub-tropical and temperate oral crops from a landscape design
point of view is the seasonality of owering, and the respective
environmental stimuli that trigger owering. In high latitudes,
day length (photoperiod) and temperature (vernalization) are
the predominant cues that signal plants to enter reproductive
phase. At lower latitudes, especially in areas with distinct wet/
dry seasonal variations, the trigger is often water availability. In
areas without signicant variation in water availability, intensity
of solar radiation has been suggested as the trigger for some spe-
cies to ower (Yeang, 2007).
Bougainvillea (Bougainvillea spp.) is a very widespread and
common woody evergreen perennial that is used as a landscape
ornamental plant in South Florida and in tropical areas around
the world. It is greatly valued for its vigor and resistance to pests,
disease, and drought, in addition to its bright oral display of
colorful bracts, and variability in form as it can be planted as a
shrub, standard, espaliered, or trained onto a trellis (Kobayashi
et al., 2007).
It is a short day (SD) plant, with owers forming in apical
panicles on the current year wood (Ma and Gu, 2010). In sub-
tropical/tropical climates like South Florida, bougainvillea owers
in late spring to early summer, and in late summer to fall, when
the daylight is less than 12-h per day and night-time tempera-
tures are above 21 °C (Schoellhorn and Alvarez, 2002). Ramina
and Sachs (1979) hypothesized that owering in bougainvillea
is a function of nutrient diversion, and in further studies (Even-
Chen and Sachs, 1980) supported the theory that SD induction
is positively correlated to photosynthetic rates in mature bou-
gainvillea leaves. Ma and Gu, (2010) built on this theory and
conrmed earlier research by Steffen et al. (1988) that owering
in bougainvillea was controlled in some way by gibberellic acid
(GA) by diverting nutrient assimilates away from the apical
meristem. The GA levels are known to change in response to
photoperiod, or more specically, to the effect of far-red light
on phytochrome photoreceptors (Taiz and Zeiger, 2010). The SD
induction in Bougainvillea ‘San Diego Red’, and Bougainvillea
glabra ‘Sanderiana’ was thought to be the result of a complex
web of interactions between GA and other hormones, as well as
environmental factors such as photoperiod and light intensity
(Even-Chen and Sachs, 1980; Joiner et al., 1962).
We hypothesized that all other environmental factors being
equal, Bougainvillea ‘Afterglow’ would exhibit the same SD
inductive response as ‘San Diego Red’, with an inductive photo-
period of between 8 and 10 h. We also sought to clarify the most
inductive day length for this cultivar. The objective of Experiment
1 was to verify that Bougainvillea ‘Afterglow’ was a quantitative
short-day plant (SDP), and that owering can be suppressed by
night interruption or daylight extension. The objective of Experi-
ment 2 was to discover the length of photoperiod that was most
inductive to owering for this cultivar.
Materials and Methods
Experiment 1 was conducted in December 2015, and Experi-
ment 2 was conducted in March 2016. Established rooted cuttings
of Bougainvillea ‘Afterglow’ were used in both experiments. They
were transplanted into 10-cm pots lled with 100% coarse washed
aquarium sand. Plants were sprayed with 30 μL of ethephon (25 mL
of 1200 ppm concentration; Southern Agricultural Insecticides,
Proc. Fla. State Hort. Soc. 129:231–233. 2016.
Ornamental, Garden & Landscape Section
232 Proc. Fla. State Hort. Soc. 129: 2016.
Inc., Hendersonville, NC) to induce leaf and inorescence senes-
cence, then pruned to remove apical buds, and as far as possible
reduced to a length with seven visible lateral nodes. They were then
kept vegetative under a 14-h photoperiod, consisting of daylight
supplemented with white LED lights supplying 70 μmol·m-1·s-1
photosynthetic photo ux (PPF). To prevent nutrient deciencies,
plants were fertilized with Peters Professional Bloom Booster
(10N–30P2O5–20K2O; JR Peters, Allentown, PA) at 9.4 μg total
nitrogen once per week starting three weeks prior to the start of
the experiment. We continued to apply the fertilizer at the same
rate once per week through the remainder of the experiment. In
addition to fertilizer, plants were watered with 50 mL of tap water
(EC = 516 μS, pH = 8.3) every two days.
experiment 1. Thirty plants (six treatments with ve replicates
each) were arranged in a completely randomized design in a
greenhouse at the University of Florida Fort Lauderdale Research
and Education Center in Davie, Florida. Photoperiod treatments
were created using a 5-gallon black plastic pot inverted over
each plant to block out light. All plants were covered at 6:00 pM
and uncovered at 8:00 aM. A single 5-watt LED bulb providing
35 μmol·m-1·s-1 PPF suspended inside each pot and set on a timer
provided night interruption or day length extension. There were
three continuous photoperiod treatments: 1) 14 h (control); 2)
11- and 8-h; and 3) three night interruption treatments (8+3 h,
8+6 h, and 11+3 h). Night interruptions occurred after 3 h of
dark. Night interruption treatments were designed to match
the number of daylight hours of the continuous photoperiod
treatments. Prior experiments (data not shown) suggested that
short night interruptions (5–30 min. of light) were insufcient
to inhibit owering.
Root zone temperature (RZT) was monitored using two data-
loggers (HOBOWare Pro U12, Onset Computer Corporation,
Bourne, MA), with sensors inserted into seven random replicates.
Temperatures were logged in °C at 30-min. intervals.
Plant size was recorded at the start and end of the experiment
to calculate growth. Plant size was determined by the formula
Size = H × W1 × W2
where H = height, rounded to the nearest centimeter, W1 = maxi-
mum width of the plant to the nearest cm, and W2 = width of the
plant perpendicular to W1, to the nearest centimeter.
Growth was dened as the difference between the plant size
at the end of the experiment and at the start of the experiment.
Relative growth was calculated as the percentage ratio of growth
over initial size.
The number of inorescences on each plant was counted at
the end of day 30. One inorescence was dened as an individual
thorn-inorescence axil, regardless of how many orets were
attached to the peduncle.
experiment 2. . The experiment was repeated with the same
preparation but with continuous photoperiod treatments without
night interruption. The treatment levels were: 14 (control), 12-h,
11-h, 10-h, 9-h, and 8-h photoperiods, respectively.
Analysis of variance (ANOVA, α = 0.05) was performed using
R statistical analysis program, with number of inorescence as
the dependent variable and photoperiod treatment and relative
growth as the independent variables. Data for Experiment 1 and
Experiment 2 were analyzed separately. Root-zone temperature
was identical across all treatments so these data were omitted
from ANOVA. The average daily temperature range was 24 °C
to 32 °C. In Experiment 1, mean separation was conducted us-
ing paired t-tests to identify which treatments were signicantly
different. In Experiment 2, Tukey’s HSD was used to identify
which treatments were signicant.
Results
The number of inorescences was not signicantly affected
by the growth of the plants, and there was no interaction between
relative growth and photoperiod treatment on number of ino-
rescences (Table 1).
In Experiment 1, plants grown under 8-h and 11-h photope-
riods produced signicantly more inorescences than all other
treatments (Table 2). All night interruption treatments inhibited
owering response. Although relative growth was not signicantly
different, there was some variation between treatments. Plants
grown under the 11+3-h treatment grew the most, while those
under 8+6-h treatment grew the least. Among the three continu-
ous photoperiod treatments, the control plants (14-h) grew the
most but had the fewest owers, while those under 8-h grew the
least but had the most number of owers. Since night interruption
effectively inhibited owering, the treatments for Experiment 2
omitted night interruption and focused on narrowing the range
of photoperiod treatments.
In Experiment 2, control plants remained completely vegeta-
tive under a 14-h photoperiod (Table 3). Plants grown under an
Table 1. Effect of Photoperiod and relative growth on number of ino-
rescences on Bougainvillea ‘Afterglow’ in Dec. 2015.
Variable df Sum Sq Mean Sq F-Value P-value
Photoperiod 5 755.5 151.10 3.235 0.0295z
Relative Growth 1 42.5 42.51 0.910 0.3527
Treatments x Rel Growth 5 267.4 53.48 1.145 0.3732
zSignicant difference at α = 0.05.
Table 2. Effects of Photoperiod on number of inorescences and relative
growth of Bougainvillea ‘Afterglow’ in December 2015. Mean values
in the same column followed by the same letters are not signicantly
different at α = 0.05.
Treatment Mean number Relative
(h) of inorescences growth (%)
Control (14) 0.4 c 262.8 a
11 6.2 a 253.0 a
8 10.2 a 190.7 a
8+3 0.0 bc 216.8 a
8+6 0.0 bc 144.4 a
11+3 3.0 c 346.9 a
Table 3. Effects of photoperiods on number of inorescences of Bou-
gainvillea ‘Afterglow’ grown in Feb. 2016. Mean values in the same
column followed by the same letters are not signicantly different
at α = 0.05.
Treatment (h) Mean number of inorescences
14 (control) 0.0 b
12 5.8 b
11 8.0 b
10 8.4 b
9 11.2 a
8 13.6 a
233
Proc. Fla. State Hort. Soc. 129: 2016.
8-h photoperiod had the highest mean number of inorescences
(13.6). Plants grown under a 9-h photoperiod had the second
highest mean number of inorescences (11.2). There was no
difference in the number of inorescences produced under 12-,
11- and 10-h photoperiods. Number of inorescences appeared to
correlate with decreasing length of photoperiod between 12–8 h.
Discussion
Previous studies indicated that Bougainvillea ‘San Diego Red’
owered under SD conditions (Even-Chen et al., 1979; Even-Chen
and Sachs, 1980). The results of Experiment 1 appear to support
the hypothesis that the cultivar ‘Afterglow’ is also a SD plant that
requires photoperiods of less than 12 h to induce owering, while
the results from Experiment 2 indicate that 8 h of daylight was the
most inductive photoperiod for ‘Afterglow’. These results concur
with previous research on optimal photoperiods for owering of
bougainvillea (Schoellhorn and Alvarez, n.d.; Singh et al., 2013).
In addition, owering of ‘Afterglow’ was completely inhibited by
extending the photoperiod to 14 h either continuously or as night
interruption. This result was interesting as early studies suggested
that there was no clearly dened critical photoperiod to induce
oral initiation in bougainvillea (Joiner et al., 1962). However,
these results suggest that there may be a threshold photoperiod
to inhibit owering altogether. In addition, the inability of short
night interruptions of 5–30 min. to inhibit owering will be of
interest for further research.
Bougainvillea is an important landscape shrub mostly in the
tropics and subtropics, where annual variation of day length is at
most between 10 1/2 h and 13 1/2 h. In these areas, seasonal varia-
tion in day length creates alternating inductive and sub-inductive
photoperiods. We designate the latter sub-inductive rather than
non-inductive because the photoperiod does not reach or exceed
14 h, which would completely inhibit owering. In South Florida,
the inductive season would correspond to mid-October to mid-
February, and the sub-inductive period would be from March
through September. The tropics also encompass equatorial areas
that have a constant year-round 12-h day length. In these places,
we could consider the entire year as always sub-inductive, so
owering can take place sporadically year-round in response
to other factors such as microclimate, light intensity, nutrient
availability, and environmental stresses. In particular, the water
decit and physical stress have been found to induce owering in
bougainvillea (Fang-Yin Liu and Yu-Sen Chang, 2011; Liu and
Chang, 2011; Ma and Gu, 2010; Schoellhorn and Alvarez, n.d.).
The conrmation of inductive photoperiods for Bougainvillea
‘Afterglow’ further supports the hypothesis that oral initiation is
a function of GA. Since the stress hormones ethylene and abscisic
acid (ABA) both have complex relations with GA pathways, fur-
ther investigation of the interactions between ethylene and ABA
levels on GA in bougainvillea in relation to owering responses
under subinductive conditions should be taken.
Literature Cited
Even-Chen, Z. and R.M. Sachs. 1980. Photosynthesis as a function of
short day induction and gibberellic acid treatment in Bougainvillea
‘San Diego Red’. Plant Physiol. 65:65–68.
Even-Chen, Z., R.M. Sachs, and W.P. Hackett. 1979. Control of owering
in Bougainvillea ‘San Diego Red’ metabolism of benzyladenine and
the action of gibberellic acid in relation to short day induction. Plant
Physiol. 64:646–651.
Liu, F.-Y.and Y.-S. Chang, 2011. Ethephon treatment promotes ower
formation in bougainvillea. Bot. Stud. 52:183–189.
Joiner, J.N., R. Dickey, and T. Sheehan. 1962. Growth and owering of
Bougainvillea glabra ‘Sander’ as affected by photoperiod and levels
of nitrogen and potassium. Proc. Fla. State Hort. Soc. 75:447–449.
Kobayashi, K.D., J. McConnell, and J. Grifs. 2007. Bougainvillea.
College of Tropical Agriculture and Human Resources Publication
OF-38, University of Hawaii at Manoa, Honolulu, HI.
Koshita, Y., and T. Takahara. 2004. Effect of water stress on ower-bud
formation and plant hormone content of satsuma mandarin (Citrus
unshiu Marc.). Sci. Hort. 99:301–307.
Liu, F.-Y., and Y.-S. Chang. 2011. Ethephon treatment promotes ower
formation in bougainvillea. Bot. Stud. 52:183–189.
Ma, S., and M. Gu. 2010. Effects of water stress and selected plant growth
retardants on growth and owering of “Raspberry Ice” Bougainvillea
(Bougainvillea spectabilis). In: XXVIII International Horticultural
Congress on Science and Horticulture for People (IHC2010): Inter-
national Symposium on 937. p. 237–242.
Menzel, C.M., 1983. The control of oral initiation in lychee: a review.
Sci. Hort. 21:201–215.
Núñez-Elisea, R. and T.L. Davenport. 1994. Flowering of mango trees
in containers as inuenced by seasonal temperature and water stress.
Sci. Hort. 58:57–66.
Ramírez, F., and T.L. Davenport. 2010. Mango (Mangifera indica L.)
owering physiology. Sci. Hort. 126:65–72.
Schoellhorn, R. and E. Alvarez. 2002. Warm climate production guide-
lines for Bougainvillea. Florida Cooperative Extension Service Fact
Sheet ENH 874, University of Florida, Gainesville, FL.
Singh, R., R. Dubey, V. Sethi, and S. Singh. 2013. Effect of various
microclimatic controls on the performance of different varieties of
Bougainvillea. Indian J. Ecol. 40: 9–13.
Steffen, J.D., R.M. Sachs, and W.P. Hackett. 1988. Bougainvillea ino-
rescence meristem development: Comparative action of GA3 in vivo
and in vitro. Am. J. Bot. 75:1225–1227.
Taiz, L. and E. Zeiger. 2010. Plant physiology (5th ed.). Sinauer Associ-
ates, Sunderland, Mass.
Yeang, H.-Y. 2007. Synchronous owering of the rubber tree (Hevea
brasiliensis) induced by high solar radiation intensity. New Phytol.
175:283–289.