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Cultivation of Ganoderma lucidum: Technology and Applications

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Abstract

Ganoderma lucidum is a species in the genus of Ganoderma with numerous pharmacological effects. It is named “Lingzhi” in Chinese. Lingzhi growth includes several different stages, such as mycelium, primordium, young and mature fruit bodies, and so on, and each stage has a unique set of requirements in nutritional factors and growth parameters. The cultivation pattern of Lingzhi is the same as that for other cultivated edible mushrooms and can be divided into two major patterns based on cultivation on a solid-state medium or in a liquid-state medium. The first pattern involves the production of Lingzhi fruit bodies, spores, and mycelia biomass, and the second pattern entails the production of Lingzhi mycelia biomass only. General facilities for Lingzhi production include various buildings and equipment, such as an inoculation room, culture room, preservation room, sterilizing room, mushroom house, packing room, waste disposal room and related equipment, laboratory, and other facilities.
... Solid state medium (SSM) cultivation method is used for the production of fruiting body, spore and mycelium biomass but the Liquid state medium (LSM) is used for the cultivation by mycelia biomass only. 44 To obtain the desired products with excellent quality of mycelial biomass along with desired biochemical products in shorter time, the advance fermentation techniques are preferred choice. 2 The cultivation pattern of G. lucidum can be divided into two parts-Solid State Fermentation (SSF) and Liquid State Fermentation (LSF) on the basis of the medium on which it is cultivated. ...
... Potato-Dextrose Broth (PDB) or other formulations are used to prepare the pure culture liquid mycelial spawn. The type of solid spawn can be identified on the basis of substrate used like: grain spawn, sawdust-bran spawn, dowels, grooved woody plugged spawn etc. 44 Spawn bags for each substrate or substrate mixtures are prepared by filling up prepared mixture or substrate like wheat, sorghum, maize, saw dust in polypropylene bags of required size (preferably 1 kg. or 5 kg.) and packed tightly using heat resistant Polyvinyl chloride (PVC) tube in the mouth of the bag. ...
... 60 Due to presence of bactericidal components, Lingzhi cannot be grown on softwood species and few hardwood species like-Camphor and Eucalyptus species. 44 The tree segments are generally cut about 12-15 cm. long and 13 cm. ...
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Medicinal mushrooms have been used since olden days for the treatment of various diseases. Ganoderma lucidum or Reishi is a medicinal mushroom of significant importance which is gaining popularity due to the presence of numerous bio-active ingredients in it. Wild collection of this mushroom along with its cultivation is done worldwide to meet its everincreasing demand in the market. Although, artificial or sawdust method of its cultivation on different woody substrates is commonly used but wood log cultivation method is also in practice. The steps for its cultivation include spawn preparation, substrate preparation, fruiting body management, harvesting and storage with their own set of requirements and precautions for each step. Several healthcare, personal care, food and beverage products made of its extract are being marketed and consumed worldwide. In this review, a general understanding about the cultivation of medicinal mushroom Ganoderma lucidum, current market scenario and its potential in Indian markets are discussed.
... The billets were further tied and kept in polypropylene bags for sterilization and autoclaved (Fig. 1). The (Zhou, 2017)was also taken into consideration during this experiment.The pressure of 1.5 kg/cm 2 was maintained in steam sterilizer for 1.5-2 h. After sterilization, the bags were kept for cooling in a chamber for a day. ...
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