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NUTRITIONAL AND MEDICINAL VALUE OF PAPAYA (CARICA PAPAYA LINN.)

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NUTRITIONAL AND MEDICINAL VALUE OF PAPAYA
(CARICA PAPAYA LINN.)
Rajasekhar Pinnamaneni*
Department of Biotechnology, K L University, Greenfields, Vaddeswaram, Guntur Dt-522502,
Andhra Pradesh, India.
ABSTRACT
The different parts of the Papaya (Carica papaya Linn.) belonging to
the family Caricaceae such as leaves, seeds, latex and fruit possess
excellent medicinal properties for treatment of different ailments. The
stem, leaf and fruit of papaya contain plenty of latex. The latex from
unripe fruit contains enzyme papain, other components include a
mixture of cysteine end peptidases, chitinases and an inhibitor of serine
protease, alkaloids, glycosides, flavanoids, saponins, tannins, phenols
and steroids. This review focuses on the nutritional value,
phytochemical constituents, antioxidant and free radical scavenging
activity, treatment for dengue fever and anticancer activity of papaya.
Papaya acts as a multi-faceted plant. It is also essential to identify the
mechanism of the plant compounds and studying the active principle of
the extract. It is recommended to include the papaya in our diet as fruit
salads, fruit juice, leaf extract, decoction prepared through papaya leaves, etc.
KEYWORDS: Carica papaya, Papaya, antioxidant, free radical scavenging, anticancer.
Carica Papaya Distribution
Papaya (Carica papaya L.) belongs to the family Caricaceae comprising 31 species in four
genera of which three genera are from America (Carica, Jacaritia and Jarilla) and one from
equatorial Africa (Cylicomorpha). It is an economically important fruit crop in Hawaii,
Australia, India, Srilanka, Phiilipines and South-east Asia including Thailand. It is also
known as papaw, pawpaw, papayer (French), melonenbaum (German), lechosa (Spanish),
mamao, mamaociro (Portuguese), mugua (Chinese) and malakol (Thailand).[1] The origin of
WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
SJIF Impact Factor 6.647
Volume 6, Issue 8, 2559-2578 Review Article ISSN 2278 4357
*Corresponding Author
Dr. Rajasekhar
Pinnamaneni
Department of
Biotechnology, K L
University, Greenfields,
Vaddeswaram, Guntur Dt-
522502, Andhra Pradesh,
India.
Article Received on
19 June 2017,
Revised on 09 July 2017,
Accepted on 30 July 2017
DOI: 10.20959/wjpps20178-9947
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papaya is tropical America. Its seeds were distributed from the Caribbean to Malacca and
India by travellers and botanists in the eighteenth century. Its distribution was continued
throughout Asia and Pacific. Papaya is grown in all tropical countries and many subtropical
countries between 32° North and South latitudes but the high commercial production is found
between 23° North and South latitudes.[1]
Biology of papaya
The genus Carica is from the Latin for a kind of fig which the leaves and fruits of papaya
resemble and the specific epithet papaya probably comes from the common name for the
fruit.[2] It is a dicotyledonous, polygamous (having male, female or hermaphrodite flowers on
the same plant), diploid species with a small genome of 372 Mbp/1C[3] and nine pairs of
chromosomes.[4]
Chemical Constituents of papaya
Papaya is a valuable plant of medicinal value. Leaves, fruit, seeds, root, bark and latex of the
papaya are used as ethno medicine (Table.1).
Table 1: Chemical composition of various parts of papaya plant.[5,6,7,8]
S.No
Part of
the Plant
1
Fruit
2
Juice
3
Seed
4
Root
5
Leaves
6
Bark
7
Latex
Nutritional value of papaya
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Papaya is common man‟s fruit with a high nutritive value available at a reasonable price. It is
rich in natural vitamins, minerals and low calorific value. The low calories content (32 Kcal /
100 g of ripe fruit) makes a favourite fruit of obese people who are into weight reducing
regime. Papaya has low carotene which helps to prevent damage by free radicals compared to
other fruits but all other nutrients are present. The fruit is rich source of different types of
enzymes. Papain present in good amount in unripe fruit is an excellent aid to digestion, which
helps to digest the protein in food at acid, alkaline and neutral medium. The celiac disease
patients, who cannot digest the wheat protein gliandin, can tolerate it, if it is treated with
crude papain. Papaya has the property of tenderizing meat. This knowledge is being put to
use by cooking meat with raw papaya to make it tender and digestible.[9,10] The fermented
papaya fruit is a promising nutraceutical as an antioxidant. It improves the antioxidant
defence in elderly patients even without any overt antioxidant deficiency state at the dose of 9
g/day orally. The papaya lipase, a hydrolase enzyme tightly bonded to the water insoluble
fraction of crude papain, is considered as a “naturally immobilized” biocatalyst10. Papaya
markedly increases iron (Fe) absorption from rice meal, which was measured in parous
Indian women, using the erythrocyte utilization of radioactive Fe method. The black seeds
edible and have a sharp, spicy taste. They are sometimes ground up and used as a substitute
for black pepper. In some parts of Asia the young leaves of papaya are steamed and eaten like
spinach.[11]
Table.2: Nutritive value of 100 gm of raw papaya.
Nutrient
Value per 100 g
References
Proximates
Water
88.06 g
12,13,14,15,16,17
Energy
43 kcal
Energy
179 kJ
Protein
0.47 g
12,13
Total lipid (fat)
0.26 g
12,13
Ash
0.39 g
12,13
Carbohydrate, by difference
10.82 g
Fiber, total dietary
1.7 g
12,13,14,15
Carbohydrates
Sugars, total
7.82 g
13
Sucrose
0
13
Glucose(dextrose)
4.09 g
13
Fructose
3.73 g
13
Lactose
0
13
Maltose
0
13
Galactose
0
13
Starch
0
13
Minerals
Calcium, Ca
20 mg
12,13,16,18
Iron, Fe
0.25 mg
12,13,16,18
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Magnesium, Mg
21 mg
12,13,16,18
Phosphorus, P
10 mg
12,13,16,18
Potassium, K
182 mg
12,13,16,18
Sodium, Na
8 mg
12,13,16,18
Zinc, Zn
0.08 mg
12,13,16,18
Copper, Cu
0.045 mg
12,13,16,18
Manganese, Mn
0.04 mg
12,13,16,18
Selenium, Se
0.6 µg
Vitamins
Vitamin C, total ascorbic acid
60.9 mg
12,13,16,19
Thiamin
0.023 mg
12,13
Riboflavin
0.027 mg
12,13
Niacin
0.357 mg
12,13
Pantothenic acid
0.191 mg
12,13
Vitamin B-6
0.038 mg
12,13
Folate, total
37 µg
12,13
Folic acid
0
Folate, food
37 µg
Folate, DFE
37 µg
Choline, total
6.1 mg
Vitamin B-12
0
Vitamin B-12, added
0
Vitamin A, RAE
47 µg
12,13,16,20,10
Retinol
0
Carotene, beta
274 µg
12,13,16,20,10
Carotene, alpha
2 µg
13,16,10
Cryptoxanthin, beta
589 µg
12,13,16,20,10
Vitamin A, IU
950 IU
12,13,16,20,10
Lycopene
1828 µg
13,16,17
Lutein + zeaxanthin
89 µg
13,16,10,21
Vitamin E (alpha-tocopherol)
0.3 mg
13,11
Tocopherol, beta
0.02 mg
13,11
Tocopherol, gamma
0.09 mg
13,11
Tocopherol, delta
0.01 mg
13,11
Vitamin D (D2 + D3)
0
Vitamin D
0
Vitamin K (phylloquinone)
2.6 µg
Other
Alcohol, ethyl
0
Caffeine
0
Theobromine
0
Flavones
Apigenin
0
19
Luteolin
0
19
Flavonols
Kaempferol
0
Myricetin
0
19,17
Quercetin
0
19,17
Isoflavones
Daidzein
0
22
Genistein
0
22
Total isoflavones
0
22
Source: (National Nutrient Database for Standard Reference Release 28, 2016 USDA,
Agricultural Research Service)
Antioxidants and free radical scavenging activity
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The leaves, seeds and juice of papaya show free radical scavenging and antioxidant activity.
The antioxidant activity of various fractions (ethanol, petroleum ether, ethyl acetate, n-
butanol and aqueous extract) from seeds of C.papaya showed that ethyl acetate and n-butanol
fractions demonstrated antioxidant and free radical scavenging activity than other
fractions.[23] Papaya juice is an efficient scavenger of highly reactive hydroxyl radicals
(OH)[24], which significantly decreased the lipid peroxidation levels and increased the
antioxidant activity in rats.[25] The leaf extract of papaya evidenced significant antioxidant
and free radical scavenging potential.[26] The peroxidase is present in the unripe fruit of
papaya but it gradually decreased after fruit ripening.[27] Whereas, the pulp of papaya is rich
in benzyl glucosinolate in the premature stage, which is present in the seed after fruit
ripening.[28] The benzyl glucosinolate is hydrolyzed to benzyl isothiocyante (BITC). The seed
extract of papaya demonstrated rich source of BITC.[29]
The major groups of phytochemicals that have been suggested as a natural source of
antioxidants may contribute to the total antioxidant activity of plant materials including
polyphenols, carotenoid and traditional antioxidant vitamins such as vitamin C and E.
Antioxidant is any substance that when present at low concentration compared to those of an
oxidisable substrate signifcantly delays or prevents oxidation of that substrate.[30] Antioxidant
functions are associated with decreased DNA damage, diminished lipid peroxidation,
maintained immune function and inhibited malignant transformation of cells.[31] Several
studies showed that phenolic compounds are the major bioactive phytochemicals with human
health benefits.[32] In fact, many authors have reported a direct relationship between total
phenolic content and antioxidant activity in numerous seeds, fruits and vegetables.[33]
The extract with the lowest β-carotene degradation rate exhibit the highest antioxidant
activity. All extracts had lower antioxidant activities than had standard -tocopherol). The
highest antioxidant activity among the samples was observed in unripe fruit whereas seed had
the lowest antioxidant activity. Result showed that there was considerably variation in the
antioxidant activities where it ranges from the lowest of 58% to the highest of 91% where the
orders of the antioxidant activity are as follow: α-tocopherol > unripe fruit > young leaves >
ripe fruit > seed (Table.3).
Reactive scavenging activity
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The idea of a single measurement of total antioxidant capacity is insuffcient. There is various
antioxidant activity methods have been used to evaluate and compare the antioxidant activity
of foods. Therefore, in this study, radical scavenging activity was determined for the selected
parts of papaya plant. Being a stable free radical, the DPPH assay is a simple and rapid
method frequently used to evaluate the ability of antioxidants to scavenge free radicals. It
gives reliable information concerning the antioxidant ability of the tested compounds to act as
free radical scavengers or hydrogen donors.[34]
This showed that the young leaves exhibit a strong scavenging activity and it has been
reported that phytochemicals especially plant phenolics constitute a major group of
compounds that act as primary antioxidant (Table.3). Their protection mechanisms are
through the reaction with the oxygen radicals, superoxide radicals and lipid peroxyl
radicals.[35]
Total phenolic content and total favonoid content
Phenolic compounds are widely distributed in plants[36], which have gained greatly attention,
due to their antioxidant activities and free radical-scavenging abilities, which potentially have
beneficial implications for human health.[37]
The total phenolic content (TPC) was observed in the selected papaya plant as: young leaves
> unripe > ripe > seed. The result also indicates that the young leaves contained high phenolic
content that may provide good sources of dietary antioxidant. For this reason, it is obvious
that TPC present in the samples have strong effects against the scavenging activity rather than
discoloration of β-carotene (Table.3). However, the radicals scavenging activity is not only
due to the phenolic content itself, but with other various antioxidant compounds.[38] They
respond differently depending on the number of phenolic groups that they possess.[39] More to
the point, TPC does not incorporate necessarily to all the antioxidants that may present in the
extracts. Therefore, sometimes there is a vague correlation between TPC and antioxidant
activity of several plant species.[40]
In recent years, studies have shown that papaya fruit contains not only vitamins and other
nutrients but also contains biologically flavonoids.[41] Previous study reported that antioxidant
activity of plant material is very well correlated with thecontent of phenolic compounds.[42]
Contribution of phenolic compounds is one of the mechanisms of the overall antioxidant
activities. This mainly due to their redox properties involve in the plant material. Generally,
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the mechanisms of phenolic compounds for antioxidant activity are neutralizing lipid free
radicals and preventing decomposition of hydroperoxides into free radicals.[36] The study
clearly indicates that it is vital to measure the antioxidant activity using various radicals and
oxidation systems and to take both phenolic content and antioxidant activity into account
while evaluating the antioxidant potential of plant extracts. The results obtained in this work
have considerable value with respect to the antioxidant activities of the selected parts of the
papaya plant. In brief, by taken into account all the parameters measured, antioxidants were
highly remarkable in the sequence of young leaves > unripe fruit > ripe fruit > seed (Table.3).
Table. 3: Antioxidant activity, DPPH radical scavenging activity, total phenolic content
and total flavanoid content[43]
Papaya
plant
material
Antioxidant
activity (%)
EC50 (mg/ml)
DPPH radical
scavenging activity
Total Phenolic
content
Total Flavonoid
content
Ripe
88.12
6.5 ± 0.01
272.66 ± 1.53
92.95 ± 7.12
Unripe
90.67
4.3 ± 0.01
339.91 ± 9.40
53.44 ± 6.63
Seed
58.97
1.0 ± 0.08
30.32 ± 6.90
59.54 ± 12.23
Leaves
90.01
7.8 ± 0.06
424.89 ± 0.22
333.14 ± 1.02
Standard
96.73
Medicinal value of Papaya
Papaya contains two important biologically active compounds vis: hymopapain and papain
which are widely used for digestive disorders.[44] It showed that papaya-derived papain,
caricain, chymopapain and glycine endopeptidase can improve acidic pH conditions and
pepsin degradation. Other active compounds of papaya are lipase, or CPL, a hydrolase, which
is tightly bonded to the water-insoluble fraction of crude papain and is thus considered as a
naturally immobilized” biocatalyst.[45] According to the folk medicine, papaya latex can cure
dyspepsia and also applicable for external burns and scalds.
Seeds and fruits are excellent antihelminthic and anti-amoebic.[46] Dried and pulverized
leaves are sold for making tea; also the leaf decoction is administered as a purgative for
horses and used for the treatment of genito-urinary system.
Unripe and semi- ripe papaya fruits are ingested or applied on the uterus to cause abortion.
However, the consumption of unripe and semi-ripe papaya fruits could be unsafe during
pregnancy, but consumption of ripe fruits during pregnancy causes no risk.[47] The latex, ripe
fruits, unripe fruits, seeds, seeds juice, root, leaves, flower and stem bark of papaya are used
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as antimicrobial, anthelmentic, antimalarial, antifungal, anti-amoebic, hepatoprotective, male
and female antifertility, immunomodulatory and against histminergic.[48]
Papaya against Dengue
Papaya leaves were collected and thoroughly washed with water. The leaves were grinded
and as the taste of papaya leaves extract was very bitter, some amount of sucrose was added
for easy administration. About 25 mL of leaves extract was administrated orally, twice daily
for five consecutive days. Before extract administration, the patient blood was evaluated for
total platelets counts, after 24 hours each time. After extract administration the patient blood
was rechecked for platelets counts again for five consecutive days. After which the patient
starts improvement. Before administration of leaves extracts, the patient blood test have been
taken. From the tests results it was observed that PLT, WBC and NEUT decreased from
normal level. After the infection the patient immediately felt fatigue and fever, these
symptoms aggravated over the night. Different antibiotics and anti-malarial drugs were
administrated orally and intravenously, but no successful results.
This case report shows the activity of papaya leaves extract against Dengue fever. The
papaya leaves extract in water was given to the patient twice daily. The patient started
vomiting as eating, so physicians recommended fruits and fruit juices. 25 mL of extract was
given to the patient in the morning and evening. After two consecutive days the blood
samples were checked for different parameters. In the first blood report, it was observed that
the PLT count, WBC and NEUT increased to 73 X103/µL, 3.8 X103/µL and 56.0%,
respectively. With similar dose of extracts, on the next day the blood report indicated that the
PLT count reached to120 X103/µL while WBC and NEUT reach to 4.4 X103/µL and 64.2%,
respectively. On the third day, it was observed that PLT count (137 X103/µL), WBC (5.3
X103/µL and NEUT (71.1%) increased. In the fourth blood report (PLT: 159 X103/µL, WBC:
5.9 X103/µL and NEUT: 73.0%) and fifth report (PLT: 168 X103/µL, WBC: 7.7 X103L and
NEUT: 78.3%) the PLT, WBC and NEUT reached their normal levels.
The Dengue fever repeats every year and causes several deaths. The rise of PLT count in the
present case from 55X103/µL to 168X103L indicates the activity of papaya leaves extract.
However, this is a preliminary work and more works on isolating the active compounds from
this valuable species are needed which may help in control of such infectious diseases.[49]
Phytochemicals in papaya with reported anticancer activities
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Papaya contains a broad spectrum phytochemicals including enzymes (in the latex),
Carotenoids (in fruits and seeds), alkaloids (in leaves), phenolics (in fruits, leaves, and
shoots), glucosinolate (in seeds and fruits).[48, 50] Some important Phytochemicals found in C.
papaya are Lycopene, Betacarotinoid, Benzylisothyocynate, Betacryptoxanthin,
Benzylglucosinolate, chlorogenic acid, caffeic acid, protocatachuic acid, Quercetin, Among
more than 5000 compounds from plants that have been identified to be associated with
anticancer properties[51], three groups of bioactive compounds-phenolics, Carotenoids, and
glucosinolate-have attracted considerable interest in anticancer studies. Pure compounds of
these three groups have been extensively researched in vivo and in vitro studies on many
types of cell lines for their potential effects in cancer treatment and prevention. These
bioactive compounds act via multiple mechanisms such as cancer cell signalling,
proliferation, apoptosis, migration, invasion, as well as angiogenesis and carcinogen
elimination[51, 52, 53, 54, 55, 56, 57, 58] to exhibit in vitro and in vivo anticancer activities.
Papaya is rich source of enzyme papain, effective against cancer
Papain (EC 3.4.22.2) is an endolytic plant cysteine protease enzyme which is isolated from
papaya latex.[59] Papain is a relatively heat-resistant enzyme, with an optimal temperature
range of 60 and 70 °C.[60] Papain prefers to cleave in between at hydrophobic-(Arg or Lys)
cleaves here -(not Val). Hydrophobic is Ala, Val, Leu, Ile, Phe, Trp, or Tyr.[61, 62] The unique
structure of papain gives its functionality that helps to understand how this Protieolytic
enzyme works and it‟s useful for a variety of purposes.[63]
Figure 1: Papain Structure.[63]
The protein is stabilized by the three disulfide bridges. Its three-dimensional structure
consists of two distinct structural domains with a cleft between them. The active site,
consisting of a cysteine and a histidine, lies at the surface of the cleft. Apart from four short
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α-helical segments and one short segment of β-structure, the conformation of the chain is
irregular.
Many cancer cells are undetected for many years as they have a protective coating of fibrin.
Papain breaks down that fibrin coat of cancer cell wall. So ultimately it helps against the
cancer.
Mechanism of functions of Papain
The mechanism in which the function of papain is made possible is through the cysteine-25
portion of the triad in the active site that attacks the carbonyl carbon in the backbone of the
peptide chain freeing the amino terminal portion. As this occurs throughout the peptide
chains of the protein, the protein breaks apart. The mechanism by which it breaks peptide
bonds involves deprotonation of Cys-25 by His-159. Asparagine-175 helps to orient the
imidazole ring of His-159 to allow this deprotonation to take place. Although far apart within
the chain, these three amino acids are in close proximity due to the folding structure. It is
though these three amino acids working together in the active site that provides this enzyme
with its unique functions. Cys-25 then performs a nucleophilic attack on the carbonyl carbon
of a peptide backbone.[61, 64] In the active site of papain, Cys -25 and His -159 are thought to
be catalytically active as a thiolate imidazolium ion pair. Papain can be efficiently inhibited
by peptidyl or non-peptidyl N-nitrosoanilines. The inactivation is due to the formation of a
stable S-NO bond in the active site (Snitroso- Cys25) of papain.[65]
Papaya is a store-house of cancer fighting lycopene
Lycopene is a member of the carotenoid family, which is synthesized by many plants and
microorganisms. It is a highly unsaturated open straight chain hydrogen compound consisting
of 11 conjugated and 2 unconjugated double bonds.[66, 67, 68] The red colour of many fruits and
vegetables is due to the presence of lycopene. Because of the presence of double bonds in the
structure of lycopene, it can exist in both the cis and Trans isomeric forms. Lycopene is
present in foods primarily in the all-trans isomeic form.[69] However, it can undergo mono-or
poly-isomerization by light, thermal energy, and chemical reactions to the cis isomeric form.
It is highly stable at high temperatures and can be stored.[70]
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Figure 2: Structure of lycopene.[71]
Molecular weight: 536.89, Exact mass: 536, Molecular formula: C40H56
Molecular composition: C-89.49%, H-10. 51%
Mechanism of Lycopene
Lycopene enhances cell-to-cell communication by increasing 'Gap junctions' between
cells.[71] Two major mechanisms have been proposed to explain the ant carcinogenic
activities of lycopene: non oxidative and oxidative mechanisms. Lycopene is hypothesized to
suppress carcinogen-induced phosphorylation of regulatory proteins such as p53 and Rb
antioncogenes and to stop cell division at the G0-G1 cell cycle phase.[72] Astorg and
colleagues proposed that lycopene-induced modulation of the liver metabolizing enzyme
cytochrome P4502E1 was the underlying mechanism of its protection against carcinogen-
induced preneoplastic lesions in rat liver. Preliminary in vitro evidence also indicates that
lycopene reduces cellular proliferation induced by insulin-like growth factors-which are
potent mitogens-in various cancer cell lines.[73] Regulation of intrathymic T-cell
differentiation (immune modulation) was suggested to be the mechanism for suppression of
mammary tumour growth by lycopene treatments in SHN retired mice.[74, 75] This is an
important mechanism by which cells communicate with each other, and helps to ensure
proper cell and organ functions. It also prevents uncontrolled growth of cancer cells and
modulates cell-cycle progression. The cell cycle is a highly ordered set of events that
culminates in cell division. The use of lycopene appears to be a potential anticancer strategy
of regulating the cell cycle by inhibiting abnormal cellular growth.[76] Lycopene has been
hypothesized to prevent carcinogenesis by protecting critical cellular bimolecular, including
lipids, lipoproteins, proteins, and DNA.[77, 78, 79]
Isothiocyanate found in papaya restore the cell cycle to eliminate cancer
Organo-sulfur compounds called isothiocyanate are found in papaya. In animal experiments,
isothiocyanate protected against cancers of the breast, lung, colon pancreas, and prostate, as
well as leukemia, and they have the potential to prevent cancer in humans. Isothiocyanate
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have shown them capable of inhibiting both the formation and development of cancer cells
through multiple pathways and mechanisms.[80]
Fig 3: Possible mechanisms of action of BITC as an anticancer agent.[81]
Researchers in Japan clarified the mechanisms of action in a type of isothiocyanate found in
papaya known as BITC. That underlies the relationship between cell cycle regulation and
appropriate cell death. When cancerous cells die on schedule, they are no longer a problem.
The researchers established that BITC exerted cancer cell killing effects that were greater in
the proliferating cells than in the quiescent cells.[81] Cancer cells that are proliferating are
much more dangerous than cancer cells that are in a state of dormancy.[80]
DISCUSSION
C. papaya is a multi faceted plant originally native to southern Mexico and Central America
and now cultivated in many tropical countries. Papaya is referred to as the fruit of the angels.
Sometimes known as a tree melon or pawpaw, papaya is known not just for its rich sweet
flavor, but also for its use as a meat tenderizer. Fruit is sweet and succulent with satiny
consistency. Slice open a papaya and see hundreds of shiny black seeds that all need to get
there start in life from the nutrition found in the fruit. This implies that fruit must be power
packed. Initially green and somewhat bitter in taste, papayas are butter-yellow when fully
ripened and shaped like a pear. Their pale-orange flesh has dozens of small, black, gelitonous
seeds at the center, similar to a melon. Unripe papaya is used in some areas of the world as a
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vegetable substitute, but is not recommended as a food when green, unless
cooked. Recommended ways to eat papaya includes its juice, which is sometimes added to
other natural fruit juices because of its pleasing taste, but it's also wonderful in salads, salsa,
and, of course, all by itself.
Papaya is very low in Saturated Fat, Cholesterol and Sodium. It is a good source of dietary
fibre and potassium, and a very good source of Vitamin A, Vitamin C and folate. Vitamin C
is one of the strong points of papaya, providing a whopping 144% of the daily recommended
value per serving, which is great as an infection fighter as well as a free radical-scavenging
antioxidant. Other vitamins include 31% of the daily value in vitamin A, required for healthy
skin, mucous membranes, and vision, and especially effective against macular degeneration.
Papaya provides 13% of the daily recommended value in folate, and good amounts of fibre
and potassium, a cell and body fluid component that helps control heart rate and blood
pressure.
The B vitamins in papayas such as folic acid, pyridoxine (vitamin B6), riboflavin, and
thiamin (vitamin B1) are called "essential" because they're required by the body, but not
produced within, so they are required through the diet to provide what is to be metabolized,
thus including foods like papaya in your diet is important.
Papaya is a natural remedy for many ailments, including atherosclerosis, heart disease,
and rheumatoid arthritis, and helps keep digestive and immune systems healthy. Papaya also
contains the flavonoid beta carotene, which studies have proven to help protect against lung
and mouth cancers. Other flavonoids, namely lutein, zeaxanthin and cryptoxanthins, have
potent antioxidant properties against free radicals that can wear down your body and cause
premature aging and degenerative diseases. Papaya contains 212 amino acids and several
enzymes, including papain, a proteolytic enzyme that has an anti-inflammatory effect on the
stomach, including swelling and fever that can develop post-surgery. Juice of papaya seeds
plays an essential role to protect kidney from becoming dysfunctional because seeds contains
flavonoids and phenolic, which provides prevention from germs of such diseases. Besides
this papaya seeds can also protect from number of infections and could also be used to clean
intestines insects. Its seeds can be used with milk to avoid typhoid disease and it can also cure
from hemorrhoids-kind diseases and dengue fever.
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Papain helps proteins digest faster, which discourages acid reflux, and has demonstrated
effectiveness in treating ulcers and even relieving irritable bowel syndrome. Papaya seeds
have been used in folk medicine to treat parasite and ringworm infections. Papaya is an
effective anti-cancer agent against cervix, breast, liver, lung and pancreas cancers. Studies
were carried out by using a tea made from the extracts of dried papaya leaves and put them in
dishes which contain cancerous cells and found that the papaya leaf extract boosted the
production chemicals that regulate the immune system. Cancer can be eradicated through
daily intake of papaya, as it contains chemical integrates such as lycopene and others.
CONCLUSION
C. papaya is a multi faceted plant originally native to southern Mexico and now cultivated in
many tropical countries. Fruit is sweet and succulent with satiny consistency. Slice open a
papaya and see hundreds of shiny black seeds that all need to get there start in life from the
nutrition found in the fruit. This implies that fruit must be power packed. Papaya is referred
to as the fruit of the angels. It is also imperative to identify the mechanism of the plant
compounds and studying the active principle of the extract. The papaya plant has been touted
by traditional hearlers for counties as source of powerful medicine. Papaya possesses rich
source of phytochemicals which includes vitamins, antioxidants, flavanoids, polyphenols,
several minerals and having some important enzyme like papain, lycopene, Isothyocynate
and some proteolytic enzyme which help to treat health problems and hence, regular intake of
papaya will improve our health by quenching the free radicals generated in the body and
enhance our immune system to fight against the foreign pathogens. Papaya promotes immune
system. It is a potent cancer fighter that is highly effective against hormone related to cancer
as well as other cancers. Papaya can stop the growth of cancer cell halt metastasis and
normalized cell cycle. Thus, intake of papaya as fruit salads, fruit juice, leaf extract,
decoction prepared through papaya leaves, etc. should be a part of our diet.
ACKNOWLEDGEMENTS
The author thanks Management, K L University, Vaddeswaram for their valuable support.
REFERENCES
1. Nakasone HY, Paull RE. Tropical Fruits. CAB International, Wallingford, England, 1998;
445.
2. Du Puy DJ, Telford IRH. Caricaceae. Chapter 30: In Flora of Australia, Oceanic Islands
2. Australian Government Publishing Service, Canberra, Australia, 1993; 50: 163-164.
www.wjpps.com Vol 6, Issue 8, 2017.
2573
Pinnamaneni. World Journal of Pharmacy and Pharmaceutical Sciences
3. Arumuganathan K, Earle ED. Nuclear DNA content of some important plant species.
Plant Mol. Biol. Rep, 1991; 9(3): 208-218.
4. Bennett MD, Leitch IJ. Nuclear DNA Amounts in Angiosperms: Progress, Problems and
Prospects. Ann Bot, 2005; 95(1): 45-90.
5. Bruneton J. Carica papaya, In: Pharmacognosy, phytochemistry of medicinal plants, Tech
Docu Fra, 1999; 2: 221-223.
6. The Wealth of India-A dictionary Indian raw materials and industrial products: Raw
material series, Ca-Ci, publications and information directorate, CSIR, 1992; 3: 276-293.
7. Nadkarni KM. Indian material medica, Pop Pra Pvt Ltd, Bombay, 1954; 1: 273-277.
8. Vijay Y, Pradeep KG, Chetan CS, Anju G, Bhupendra V. Carica papaya Linn: An
Overview. Int. j. herb. Med, 2014; 2(5): 1-08.
9. Philip T, Chen TS. Quantitative analyses of major carotenoid fatty acid esters in fruits by
liquid chromatography: Persimmon and Papaya. J. Food Science, 1988; 53(6):
1720-1722.
10. National Institutes of Health (NIH) Carotenoid analyses of U.S. foods, Food Composition
Laboratory, 1997.
11. Franke AA, Suzanne M, Lacey R, Custer LJ. Tocopherol and tocotrienol levels of foods
comsumed in Hawaii, J Agric Food Chem, 2007; 55(3): 769-778.
12. Produce Marketing Association (PMA). Nutrient Content of Papaya, 1984.
13. Nutrient Data Laboratory, ARS, USDA National Food and Nutrient Anlaysis Program
Wave 12i, 2008 Beltsville MD.
14. Vollendorf N, Marlett J. Comparison of Two Methods of Fiber Analysis of 58 Foods. J.
Food Comp. Anal, 1993; 6(3): 203-214.
15. Mahattanatawee K, Manthey JA, Luzio G, Talcott ST, Goodner K, Baldwin EA. Total
antioxidant activity and fiber content of select Florida-grown tropical fruits, J Agric Food
Chem, 2006; 54(19): 7355-7363.
16. Wall MM. Ascorbic acid, vitamin A, & mineral composition of banana & papaya
cultivars grown in Hawaii. J. Food Comp. Anal, 2006; 19(5): 434-445.
17. Lako J, Trenerry VC, Wahlqvist M, Wattanapenpaiboon N, Sotheeswaran S, Premier R.
Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of
Fijian fruit, vegetables and other readily available foods. Food Chemistry, 2007; 101:
1727-1741.
www.wjpps.com Vol 6, Issue 8, 2017.
2574
Pinnamaneni. World Journal of Pharmacy and Pharmaceutical Sciences
18. Miller-Ihli NJ. Atomic absorption and atomic emission spectrometry for the
determination of the trace element content of selected fruits consumed in the United
States. J. Food Comp. Anal, 1996; 9(4): 301-311.
19. Franke AA, Custer LJ, Arakaki C, Murphy SP. Vitamin C and flavonoid levels of fruits
and vegetables consumed in Hawaii. J. Food Comp. Anal, 2004; 17: 1-35.
20. Philip T, Chen TS. Development of a method for the quantitative estimation of
provitamin A carotenoids in some fruits. J. Food Sci, 1988; 53(9): 1703-1707.
21. Humphries JM, Khachik F. Distribution of lutein, zeaxanthin, & related geometrical
isomers in fruit, vegetables, wheat, & pasta products. J Agric Food Chem, 2003; 51(3):
1322-1327.
22. Horn-Ross PL, Barnes S, Lee M, Coward L, Mandel E, Koo J, John EM, Smith
M. Assesing phytoestrogen exposure in epidemiologic studies: development of a database
(United States)., Cancer Causes Control, 2000; 11(4): 289-98.
23. Zhou K, Wang H, Mei W, Li X, Luo Y, Dai H. Antioxidant activity of papaya seed
extracts. Molecules, 2011; 6(8): 6179-6192.
24. Webman EJ, Edlin G, Mower HF. Free radical scavenging activity of papaya juice. Int J
Radiat Biol, 1989; 55(3): 347-351.
25. Mehdipour S, Yasa N, Dehghan G, Khorasani R, Mohammadirad A, Rahimi R, Abdollahi
M. Antioxidant potentials of Iranian Carica papaya juice in vitro and in vivo are
comparable to alpha-tocopherol. Phytother Res, 2006; 20(7): 591-594.
26. Okoko T, Ere D. Reduction of hydrogen peroxide-induced erythrocyte damage by Carica
papaya leaf extract. Asian Pac J Trop Biomed, 2012; 2(6): 449-453.
27. Pandey VP, Singh S, Singh R, Dwivedi UN. Purification and characterization of
peroxidase from papaya (Carica papaya) fruit. Appl Biochem Biotechnol, 2012; 167(2):
367-376.
28. Li ZY, Wang Y, Shen WT, Zhou P. Content determination of benzyl glucosinolate and
anti-cancer activity of its hydrolysis product in Carica papaya L. Asian Pac J Trop Med,
2012; 5(3): 231-233.
29. Nakamura Y, Yoshimoto M, Murata Y, Shimoishi Y, Asai Y, Park EY, Sato K,
Nakamura Y. Papaya seed represents a rich source of biologically active isothiocyanate. J
Agric Food Chem, 2007; 55(11): 4407-4413.
30. Halliwell B, Aeschbach R, Löliger J, Aruoma OI. The characterization of antioxidants.
Food Chem Toxicol, 1995; 33(7): 601-617.
www.wjpps.com Vol 6, Issue 8, 2017.
2575
Pinnamaneni. World Journal of Pharmacy and Pharmaceutical Sciences
31. Gropper SS, Simmons KP, Gaines A, Drawdy K, Saunders D, Ulrich P, Connell, LJ. The
freshman 15-a closer look. J Am Coll Health, 2009; 58(3): 223-231.
32. Cao G, Sofic E, Prior R. Antioxidant capacity of tea and common vegetables. J Agric
Food Chem, 1996; 44(11): 3426-3431.
33. Yang, J., Liu, R, Halim, L. Antioxidant and antiproliferative activities of common edible
nut seeds. Food Science and Technology, 2009; 42(1): 1-8.
34. Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J. Agric.
Food Chem, 2005; 53(6): 1841-1856.
35. Hatano T, Edamatsu R, Hiramatsu M, Mori A, Fujita Y. Effects of the interaction of
tannins with co-existing substances. VI: effects of tannins and related polyphenols on
superoxide anion radical and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm.
Bull, 1989; 37: 2016-2021.
36. Li BB, Smith B, Hossain MM. Extraction of phenolics from citrus peels: I. Solvent
extraction method. Sep Purif Techno, 2006; 48(2): 182-188.
37. Govindarajan R, Singh DP, Rawat AKS. High-performance liquid chromatographic
method for the quantifcation of phenolics in „Chyavanprash‟ a potent Ayurvedic drug. J
Pharm Biomed Anal, 2007; 43: 527-532.
38. Khamsah SM, Akowah G, Zhari I. Antioxidant activity and phenolic content of
Orthosiphon stamineus benth from different geographical origin. Journal of Sustainability
Science Management, 2006; 1(2): 14-20.
39. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phospho-
tungstic acid reagents. Am J Enol Vitic, 1965; 16: 144-158.
40. Tawaha K, Alali FQ, Gharaibeh M, Mohammad M, El-Elimat T. Antioxidant activity and
total phenolic content of selected Jordanian plant species. Food Chemistry, 2007; 104(4):
1372-1378.
41. Wang J, Wu FA, Zhao H, Liu L, Wu QS. Isolation of favonoids from mulberry (Morus
alba L.) leaves with macroporous resins. Afr. J. Biotechnol, 2008; 7(13): 2147-2155.
42. Velioglu YS, Mazza G, Oomah BD. Antioxidant activity and total phenolics in selected
fruits, vegetables, and grain products. J. Agric. Food Chem, 1998; 46(10): 4113-4117.
43. Maisarah AM, Nurul Amira B. Asmah R and Fauziah O. Antioxidant analysis of different
parts of Carica papaya. International Food Research Journal, 2013; 20(3): 1043-1048.
44. Huet J, Looze Y, Bartik K, Raussens V, Wintjens R, Boussard P. Structural
characterisation of the papaya cysteine proteinases at low pH. Biochem. Biophys. Res.
Commun, 2006; 341(2): 620-626.
www.wjpps.com Vol 6, Issue 8, 2017.
2576
Pinnamaneni. World Journal of Pharmacy and Pharmaceutical Sciences
45. Dominguez de Maria P, Sinisterra JV, Tsai SW, Alcantara AR. Carica papaya lipase
(CPL): An emerging and versatile biocatalyst. Biotechnol Advances, 2006; 24(5):
493-499.
46. Okeniyi JAO, Ogunlesi TA, Oyelami OA, Adeyemi LA. Effectiveness of dried Carica
papaya against Human intestinal parasitosis: A pilot study. J. Med. Fd, 2007; 10(1):
194-196.
47. Adebowale A, Garnesan AP, Prasad RNV. Papaya (Carica papaya) consumption is unsafe
in pregnancy: Fact or fable? Scientific evaluation of a common belief in some parts of
Asia using a rat model. British J Nutr, 2002; 88(2): 199-203.
48. Karishna KL, Paridhavi M, Patel JA. Review on nutritional, medicinal and
pharmacological properties of papaya (Carica papaya Linn.). Nat Prod Radiance, 2008; 7:
364-373.
49. Nisar A, Hina F, Muhammad A, Bilal H A, Ijaz M, Lubna F. Dengue fever treatment with
Carica papaya leaves extracts. Asian Pac J Trop Biomed, 2011; 1(4): 330-333.
50. Parle M, Gurditta. Basketful benefits of papaya. International Research Journal of
Pharmacy, 2011; 2(7): 6-12.
51. Huang WY, Cai YZ, Zhang Y. Natural phenolics compounds from medicinal herbs and
dietary plants: potential use for cancer prevention. Nutrition and Cancer, 2009; 62(1):
1-20.
52. Zhang Y. Cancer-preventive isothiocyanate: measurement of human exposure and
mechanism of action. Mutat Res, 2004; 555(1-2): 173-190.
53. Thornalley PJ. Isothiocyanate: mechanism of cancer Chemo preventive action.
Anticancer Drugs, 2002; 13(4): 331-338.
54. Nakamura Y, Miyoshi N. Cell death induction by isothiocyanate and their underlying
molecular mechanisms. Bio Factors, 2006; 26(2): 123-134.
55. Wu X, Zhou QH, Xu K. Are isothiocyanate potential anti-cancer drugs? Acta Pharmacol
Sin, 2009; 30(5): 501-512.
56. Wahle KWJ, Brown I, Rotondo D, Heys SD. Plant phenolics in the prevention and
treatment of cancer. Bio-Farms for Nutra Sweet, 2011; 698: 36-51.
57. Soobrattee MA, Bahorun T, Aruoma OI. Chemo preventive actions of polyphenolic
compounds in cancer. Bio Factors, 2006; 27(1-4): 19-35.
58. Tanaka T, Shnimizu M, Moriwaki H. Cancer chemoprevention by Carotenoids.
Molecules, 2012; 17(3): 3202-3242.
www.wjpps.com Vol 6, Issue 8, 2017.
2577
Pinnamaneni. World Journal of Pharmacy and Pharmaceutical Sciences
59. Abu-Alruz K, Mazahreh AS, Quasem JM, Hejazin RK, El-Qudah JM. Effect of Proteases
on Melt ability and Stretch ability of Nabulsi Cheese. Am J of Agri Biol, 2009; 4(3):
173-178.
60. Lauwers A, Scharpé S. Pharmaceutical Enzymes, drugs and pharmaceutical sciences.,
Marcel Dekker, Inc., New York-Basel-Hong Kong, 1997; 84.
61. Menard R, Khouri HE, Plouffe C, Dupras R, Ripoll D. A protein engineering study of the
role of aspirate 158 in the catalytic mechanism of papain. Biochemistry, 1990; 29:
6706-6713.
62. Cornell HJ, Doherty W, Stelmasiak T. Papaya latex enzymes capable of detoxification of
gliadin. Amino Acids, 2001; 38(1): 155-165.
63. Mitchel RE, Claiken MI, Smith ELJ. The complete amino acid sequence of papain. The
Journal of Biological Chemistry, 1970; 245(14): 3485-3492.
64. Tsuge H, Nishimura T, Tada Y, Asao T, Turk D. Inhibition mechanism of cathepsin
Lspecific inhibitors based on the crystal structure of papain-CLIK148 complex. Biochem.
Biophys. Res. Commun, 1999; 266(2): 411-416.
65. Xian M, Chen X, Liu Z, Wang K, Wang PG. Inhibition of papain by s-nitrosothiols. J
Biol Chem, 2000; 275(27): 20467-20473.
66. Stahl W, Schwarz W, Sundquist AR, Sies H. Cis-Trans, Isomer of Lycopene and Beta-
carotene in human serum and tissues. Arch. Biochem. Bioph, 1992; 294: 173-177.
67. Knachik F, Caryallo L, Bernstein PS, Muir GJ, Zhao DY, Katz NB. Chemistry,
distribution and metabolism of tomato Carotenoids and their impact on human health.
Exp Biol Med (Maywood), 2002; 227(10): 845-851.
68. Rao AV, Mira MR, Rao LG. Lycopene. Adv Food Nutr Res, 2006; 51: 99-164.
69. Clinton SK. Lycopene: Chemistry, biology and implications for human health and
diseases. Nutr Rev, 1998; 56(2): 35-51.
70. Agarwal A, Shen H, Agarwal S, Rao A. Lycopene content of tomato products, its
stability, bioavailability, and in vivo antioxidant properties. J Med Food, 2001; 4(1):
15-49.
71. Gali-Muhtasib H, Bakkar N. Modulating cell cycle: Current applications and prospects
for future drug development. Curr. Cancer Drug Targets, 2002; 2(4): 309-336.
72. Obermuller-Jevis UC, Olano-Martin E, Corbacho AM, Eiserich JP, Van Der Vliet A,
Valacchi G. Lycopene inhibits the growth of normal human prostate epithelial cells in
vitro. J. Nutr, 2003; 133(11): 3356-3360.
www.wjpps.com Vol 6, Issue 8, 2017.
2578
Pinnamaneni. World Journal of Pharmacy and Pharmaceutical Sciences
73. Levy J, Bosin E, Feldmen B, Giat Y, Miinster A, Danilenko M. Lycopene is a more
potent inhibitor of human cancer cell proliferation than either á-carotene or â-carotene.
Nutr Cancer, 1995; 24(3): 257-266.
74. Nagasawa H, Mitamura T Sakamoto S, Yamamoto K. Effects of lycopene on spontaneous
mammary tumour development in SHN virgin mice. Anticancer Res, 1995; 15(4):
1173-1178.
75. Kobayashi T, Iijima K, Mitamura T, Toriizuka K, Cyong JC and Nagasawa H. Effects of
lycopene, a carotenoid, on intrathymic T cell differentiation and peripheral CD4/CD8
ratio in a high mammary tumor strain of SHN retired mice. Anticancer Drugs, 1996; 7(2):
195-198.
76. Agarwal S, Rao AV. Tomato lycopene and low density lipoprotein oxidation: A human
dietary intervention study. Lipids, 1986; 33(10): 981-984.
77. Matsushima NR, Shidoji Y, Nishiwaki S, Yamada T, Moriwaki H, Muto Y. Suppression
by Carotenoids of microcrystal-induced morphological changes in mouse hepatocytes.
Lipids, 1995; 30: 1029-1034.
78. Pool-Zobel BL, Bub A, Muller H, Wollowski I, Rechkemmer G. Consumption of
vegetables reduces genetic damage in humans: First result of a human intervention trial
with carotenoid-rich foods. Carcinogenesis, 1997; 18(9): 1847-1850.
79. Rao AV, Agarwal S. Bioavailability and in vivo antioxidant properties of lycopene from
tomato products and their possible role in the prevention of cancer. Nutr Cancer, 1998.
31(3): 199-203.
80. Barbra LM. Papaya is tasty way to fight cancer and poor digestion Forum of nutrition.
Bhattachrjee SK. Carica papaya. In: Hand Book of Medicinal Plant, edition: 3rd Revised,
editors: Shashi Jain, (Pointer Publisher, Jaipur), 2001; 1-71.
81. Chinthalapally VR. Benzyl Isothiocyanate: Double Trouble for Breast Cancer Cells. Cancer
Prev Res, 2013; 6(8): 760-763.
... It has been chosen as the earliest crop species which has gone under genome sequencing (Wei et al. 2008). Papaya leaves are the only known source of natural cure against dengue fever (Ahmad et al. 2011;Pinnamaneni, 2017). Different portions of this particular species (Carica papaya L.) can also be exploited as an effective natural antidote against viral diseases (Ahmad et al. 2011). ...
... Another cancer-fighting agent lycopene (which is under the carotenoid family) is also present in papaya. Again, a phytochemical isothiocyanate found in papaya which can restore the cell cycle to destroy cancerous cell (Pinnamaneni, 2017). ...
... So, biochemical traits have great importance while selecting any accession for varietal development. Moisture percentage, carbohydrate, protein, fiber content, total antioxidant activity, total phenolic compounds, flavonoids, carotenoids, lycopene content, chlorophyll content, vitamin C and minerals like sodium (Na), potassium (K), magnesium (Mg) and Iron (Fe) are the most important chemical properties of papaya (Pinnamaneni, 2017). ...
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Papaya is one of the most valuable horticultural crops in Bangladesh. Still there is a lack of variety of papaya at farmers level. Only two released varieties of papaya (BU Papaya-1 and BARI Papaya-1) is available in Bangladesh. Therefore, this study was undertaken to characterize the indigenous and exotic papaya germplasm at morphological, biochemical and molecular level to make the desired traits available in the future papaya breeding program. Among the studied 29 genotypes, 22 genotypes consisted of a population which had 100% fruit bearing plants. The genotypes G5, G11, G14 and G27 were found to be suitable for kitchen garden purpose while G1 and G6 were found to be suitable for commercial cultivation based on morphological and biochemical characteristics. All the nine Simple Sequence Repeat (SSR) markers were found to be polymorphic. The Polymorphism Information Content (PIC) value was above 0.5 suggesting that all these markers were highly informative for the genetic study of papaya. But, among them, the marker P3K1024CC detected the maximum number of alleles (8) along with the highest PIC value (0.77) and detected the second highest gene diversity (0.79). Based on the molecular analysis, the total population was divided into six sub-populations. Among them, population VI had the maximum number of pure genotypes (6). The molecular variation among the population was 11% and within the population was 89%. The results of combined analysis suggested that some genotypes were always grouped together, regardless of the strategy used to study genetic diversity. These are the duplicate or close genotypes which must be considered as a single accession when assessing germplasm conservation or selecting germplasm for crop improvement. The results indicated moderate to high variation in the studied genotypes. These variations can be utilized to develop papaya variety with desired characteristics.
... Other vitamins (aside from vitamin C) found in papaya, according to (21), include 31 percent of the daily value in vitamin A, which is needed for healthy skin, mucous membranes, and vision, and is notably beneficial against macular degeneration. ...
... Papaya also includes beta carotene, a flavonoid that has been shown in trials to help protect against lung and oral cancers. Other flavonoids, such as lutein, zeaxanthin, and cryptoxanthins, are powerful antioxidants that fight free radicals, which can wear down your body and cause premature aging and degenerative disease (21). ...
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... Many natural products rich in polyphenols, and strong antioxidant activity have been studied for their positive benefits in the treatment of hyperlipidemia. The presence of these bioactive compounds as well as the significant antioxidant activity in vitro has been observed in the pulp of papaya (34). Tijjani et al. (2020) reported that the phytochemicals detected in papaya fruit consisted of alkaloids, saponins, and flavonoids (25). ...
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... Moreover, significant decreases in serum cholesterol and triacylglycerol levels were observed in comparison to untreated diabetic rats after a 4-week administration of 3 g/100 mL of papaya leaf extract to diabetic rats [31]. The whole plant has high medicinal value as a result of its broad spectrum of vitamins, enzymes, and other active compounds [13]. ...
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... In recent years, increasing attention has been attracted to metabolite profiles of endophytic fungi from medicinal plants (Kaul et al., 2012). Papaya, Carica papaya L. (papaya), an edible and medicinal plant cultivated in tropical and subtropical regions, has been used as topical dressings for ulcer and dermatitis treatment, has gastrointestinal uses such as anti-helminthic and antibacterial activity treatments, has been used as anti-arthritis treatment, and has traditional uses for fertility control (Krishna et al., 2008;Pinnamaneni, 2017). ...
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... It is low in calorie and rich in vitamins and minerals. Moreover, the ripe papaya fruits contains water (88 g/100g), carbohydrate (10.82 g/100g) and Protein (0.47g/100g) and are known to be a rich source of phyto-nutrients, potassium (182 mg/100 g), calcium (20 mg/100g), dietary fibers (1.7g/100g), vitamin A (950 IU) and Vitamin C (60.96 mg/100g) (Pinnamaneni, 2017). Apart from using as a dessert fruit, a variety of products such as tutti-frutti, jam, jelly, sauce, toffee, bar, leather, pickles, crystallized fruits and dried slices may also be prepared from unripe or ripe papaya fruits. ...
... Air-dried Carica papaya. L was used for the quantitative determination of ash values, extractive values, moisture content, swelling index, foaming index and foreign organic matter, via standard methods [16] . The total Ash value for a crude drug is not always reliable since there is a possibility of the presence of non-physiological substances such as earthy matters. ...
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Papaya (Carica papaya Linn.) is generally known for its food and health benefits all through the world. The restorative properties of papaya leafy foods parts of the plant are additionally notable in customary arrangement of medication. Since, each piece of papaya tree has financial worth; it is developed on business scale. During the most recent couple of many years extensive advancement has been accomplished with respect to the natural movement and therapeutic use of papaya and now it is considered as significant nutraceutical organic product plant. Phytochemical analysis of papaya leaves in presence of absences in natural for different modern and drug items for different illnesses. The fluorescence analysis presence of chemical nature. Inorganic elements iron concentration high levels and physicochemical parameters maximum Ash value 11.2% and Minimum value Sulphated Ash 9.2%. Medicinal papaya leaf powdered and products thereof are used in many countries in the treatment and management of diabetes. In the current survey dietary benefit of the foods grown from the ground properties of its different parts have been examined to give aggregate data on this multipurpose business organic product crop.
... This plant family is already known to be effective against several diseases [7]. The leaves of papaya contain many active ingredients such as papain, chymopapain, cystatin, tocopherol, ascorbic acid, flavonoids, cyanogenicglucosides and glucosinolates, which can increase the total antioxidant pool in blood, and consequently reduce lipid peroxidation level, [8]. ...
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The active cysteine of papain was labeled with ¹⁴C-iodoacetate and the cystine residues were reduced and coupled with unlabeled iodoacetate. The heptacarboxymethyl papain was then maleyalated and hydrolyzed with trypsin. Key peptides were isolated from this hydrolysate which have permitted completion of the amino acid sequence of the protein. Thus, an earlier tentative and incomplete version of this sequence has been corrected and shown to be in accord with the studies of Drenth et al. (Drenth, J., Jansonius, J. N., Koekoek, R., Swen, H. M., and Wolthers, B. G., Nature, 218, 929 (1968)) by x-ray crystallographic methods.
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Problem statement: Boiled white brined cheese (Nabulsi cheese) is the mostly consumed cheese in Jordan; this cheese should show meltability and high stretchability in order to fit in the production of high quality Kunafa and other popular local sweets and pastries. However, these characteristics are rarely available when usual processing and preservation methods were used. Approach: This study was based on the hypothesis that it would be possible to imply meltability and stretchability to the cheese by proteolytic enzymes to the original brine that may specifically act on cross linking bonds of casein. In this study, six commercial proteases were used. Results: It was found that Nabulsi cheese treated with papain developed an outstanding fibrous structure, this gives superiority in the application in kunafa, pizza and pastries. The meltability and stretchability of Nabulsi cheese treated with papain were still excellent after 4 weeks of storage; this indicated the restricted enzyme action, probably due to high salt concentrations (18%) in storage brine. Conclusion: Use of proteolytic enzymes to induce meltability and stretchability of Nabulsi cheese was proved to be an efficient method.
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Papaya (Carica papaya linn) is well known for its exceptional nutritional and medicinal properties throughout the world. From the times immemorial, the whole Papaya plant including its leaves, seeds, ripe and unripe fruits and their juice is used as a traditional medicine. The fruit has a large oval shape, yellowish-green skin and yellow flesh. Nowadays, Papaya is considered as a Nutraceutical fruit due to its multi-faceted medicinal properties. The prominent medicinal properties of Papaya include Anti-fertility, Uterotonic, Diuretic, Anti-hypertensive, Hypolipidemic, Anti-helmintic, Wound-healing, Anti-fungal, Anti-bacterial, Anti-tumor and Free radical scavenging activities. Phytochemically, the whole plant contains enzymes (Papain), carotenoids, alkaloids, monoterpenoids, flavonoids, minerals and vitamins. In the present review article, a humble attempt is made to compile all the strange facts available about this tasty fruit. This tasty fruit of Papaya is popular among family members of all ages for the delicious dishes derived from it.
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This study was conducted to compare the total antioxidant activity (TAA), total phenolic content (TPC) and total flavonoid content (TFC) from the different parts of papaya tree including their ripe and unripe fruit, seeds and the young leaves. Two methods namely DPPH radical scavenging activity and β-carotene bleaching assay were used to determine the TAA, whereas TPC was determined by Folin-Ciocalteu's method while TFC by aluminium trichloride (AlCl3). For these purposes, methanolic extracts (80%) were prepared. The results showed that the highest antioxidant activity through β-carotene bleaching assay was observed in unripe fruit (90.67 ± 0.29%) followed by young leave, ripe fruit and the seed. In other hand, young leaves exhibited a significant higher scavenging effect compared to others and the dose required in reducing the absorbance of DPPH control solution by 50% (EC50) was calculated at 1.0 ± 0.08mg/ml. The EC50 values were 4.3 ± 0.01mg/ml, 6.5 ± 0.01mg/ml and 7.8 ± 0.06mg/ml for unripe fruit, ripe fruit and seeds respectively. Interestingly, both TPC and TFC also showed that young leaves had the highest antioxidant content (424.89 ± 0.22mg GAE/ 100 g dry weight and 333.14 ± 1.03mg rutin equivalent/ 100 g dry weight, respectively). Statistically, Pearson correlation showed there were positive correlations between TPC and TFC with antioxidant activity assayed by DPPH radical scavenging assay (r=0.846 and r=0.873, respectively). However there was no correlation between TPC and TFC with β-carotene bleaching activity. In brief, taken into account all the parameters measured, antioxidants were highly remarkable in the sequence of young leaves > unripe fruit > ripe fruit > seed. Nevertheless, further investigation for isolation and identification of the phytoconstituents responsible for antioxidant activity is desirable.
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Papaya (Carica papaya Linn.) is commonly known for its food and nutritional values throughout the world. The medicinal properties of papaya fruit and other parts of the plant are also well known in traditional system of medicine. Since, each part of papaya tree possesses economic value, it is grown on commercial scale. During the last few decades considerable progress has been achieved regarding the biological activity and medicinal application of papaya and now it is considered as valuable nutraceutical fruit plant. It can be chosen as a source of papain for the development of various industrial and pharmaceutical products. In the present review nutritional value of the fruit and medicinal properties of its various parts have been discussed to provide collective information on this multipurpose commercial fruit crop.
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