Isolation and growth optimisation of fresh water microalgae Asterarcys quadricellulare

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... The green microalgae, Asterarcys quadricellulare strain (KT280061) isolated from freshwater were used in this study. The species was characterized morphologically and phylogenetically in a previous study (Karthikeyan & Thirumarimurugan 2017). Large-scale cultivation of A. ...
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The rapid depletion of the world’s fossil fuel reserves and global warming issues have promoted the search for sustainable alternative energy resources. In the present investigation, large-scale cultivation of naturally isolated freshwater microalgae Asterarcys quadricellulare strain was carried out using tertiary treated municipal wastewater as a growth medium in an open HRP pond for bioethanol production. A total of 12.091 kg of dry biomass was obtained at the end of the study. The lipid extracted carbohydrate rich spent microalgae biomass was converted to bioethanol by ethanolic fermentation. The biomass was first pre-treated with different concentrations of H2SO4 and HCL hydrolysis with different temperatures and reaction times. The biomass treated with a 2.0% concentration of H2SO4, showed maximum yields of glucose 308.38 mg.g-1 at 100°C with 180 min reaction time. The hydrolysates derived from the hydrolysis of microalgae biomass were used as a substrate for fermentation by using S. cerevisiae. The obtained bioethanol was analyzed using HPLC and the purity of ethanol was 90%.
Aim of this study is uncovering potential strains of microalgae from Indore, Madhya Pradesh situated in central India with a diverse climate for sustainable production of different bio‐products. Assessing the entire biochemical profile is crucial to select species‐ specific metabolites for sustainability of algal bio‐refinery processes. In the current study, seven algal species (Coelastrum proboscideum, Desmodesmus psuedocommunis, Asterarcys quadricellulare, Ettlia texensis, Pediludiela daitoensis, Coelastrella sp., Pectinodesmus sp.) were isolated and identified using Internal Transcribed Spacer (ITS) and 18s rRNA sequences, and these species found to be from the Chlorophyta phylum. The highest biomass productivity (51.25±1.96 μg/mL/day) with chlorophyll content (33.07±0.63 μg/mL) was observed in A. quadricellulare on 21st day (P<0.0001). Biochemical profiling of isolated species showed each species is unique in its biochemical composition (total lipids, carbohydrates and proteins). Coelastrella sp. and Pectinodesmus sp. was found to be accumulating considerable quantities of (P<0.0001) carbohydrates (303.3±1.11 μg/mg dry cell weight) and lipids (212.67±2.53 μg/mg per dry cell weight), respectively. Whereas P. daitoensis was found best (P<0.0001) for protein production (205.78±2.56 μg/mg dry cell weight), on 21st day. Highest total fatty acid content was detected in Pectinodesmus sp. (115.74 μg/mg) on 21st day, which has shown good saturated fatty acid (SFA) and mono unsaturated fatty acid (MUFA) percentage, inflicting high quality biodiesel production. Confocal microscopy further supported the data. Assessing the biochemical profile at different phases of growth for each species therefore reduce the production time of biofuels as well as make this process much more cost‐effective. This article is protected by copyright. All rights reserved.
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