Article

Aloe vera Leaf Extract as a Potential Growth Enhancer for Populus Trees Grown Under in vitro Conditions

Authors:
To read the full-text of this research, you can request a copy directly from the author.

Abstract

Natural plant extracts are a cost-effective and environmental friendly alternative to synthetic plant growth regulators and phytohormones. The present in vitro study investigated the promotory activity of various concentrations (0, 10, 20 and 40 mL/L) of Aloe vera leaf extract (ALE) on the growth of hybrid aspen (Populus tremula L. × Populus tremuloides "Michx") clone T89 and aspen (Populus tremula L.) clone W52. The extract isolated from Aloe vera leaves increased the plant height and weight, number of shoots, leaves and roots, and the root length, as well as mineral concentrations of both Populus clones. The rooted plants were acclimatized in the greenhouse with 90% survival. The results showed that ALE is an efficient alternative source to improve the growth of both Populus clones under study.

No full-text available

Request Full-text Paper PDF

To read the full-text of this research,
you can request a copy directly from the author.

... Kelp extracts are well-established biostimulants within the agricultural industry (Arioli et al., 2015;Crouch and Van Staden, 1993), and are associated with a range of improvements to plant growth and yield, including; increased biomass, nutrient uptake, germination, and root growth (Battacharyya et al., 2015;Khan et al., 2009). Similarly, Aloe vera extracts have also been shown to have biostimulating effects, including increases to plant growth, biomass, root growth, and oil production (El Sherif, 2017;Hamouda et al., 2012). Both kelp and Aloe vera extracts are feature active components in the root stimulating biostimulant complex, Root Nectar® (Nutrifield Pty Ltd., Melbourne, VIC). ...
... Kelp extracts contain plant nutrients, simple and complex sugars, vitamins, amino acids, and plant hormones (Battacharyya et al., 2015;du Jardin, 2015;Hamza and Suggars, 2001), and have been shown to have beneficial effects on rooting, germination, early plant growth, and the reduction of plant stress (Battacharyya et al., 2015;Carvalho et al., 2013;Rao and Chatterjee, 2014;Sangha et al., 2014). Similarly, Aloe vera extracts contain nutrients, vitamins, enzymes, amino acid, and sugars, as well as plants sterols, gibberellins, and salicylic acid (SA), and are associated with improvements in vegetative growth (particularly root growth), plant mineral composition, and oil production (Chatterjee et al., 2013;El Sherif, 2017;Hamouda et al., 2012). Whilst the exact modes of action of these plant extracts are as yet unknown, many of their constituents have been shown to affect root growth or aspects of nutrient uptake which may impact root development in cuttings. ...
... Sugars have been shown to promote rooting in cuttings when applied to the rooting medium, by providing the plant with a carbon source (Ahkami et al., 2009), while amino acids are associated with increases to nutrient uptake via chelation (Callahan et al., 2007;Halpern et al., 2015). Although plant hormones have been identified in kelp and Aloe vera extracts (include the rooting hormone indole acetic acid (IAA), and SA, respectively), these are often in small and varied quantities (Buggeln and Craigie, 1971;El Sherif, 2017;Kingman and Moore, 1982;Sharma et al., 2011;Surjushe et al., 2008) and more research is needed to confirm their activity and effects. Additionally, Kelp and Aloe vera extracts contain hormone-like compounds which may also contribute to these effects (Davis and Maro, 1989;Stirk and Van Staden, 1996). ...
Article
Full-text available
Biostimulants are a powerful tool for optimizing the propagation efficiency of vegetative cuttings, however, their optimal application rates are often species specific. Herein, the root promoting effects of Nutrifield’s biostimulant complex Root Nectar® and the biostimulant willow bark extract were tested on vegetative cuttings from lavender (soft wood) and chrysanthemum (semi-hard wood). Application of Root Nectar® at 1 mL/L achieved consistent improvements in the formation of adventitious roots and root branching in both lavender and chrysanthemum, and 1.06 μL/L willow bark extract achieved best results for both species. The root promoting biostimulant effects of Root Nectar® and willow bark extract can be utilized to speed up propagation of soft and semi-hard wood cuttings, highlighting their applicability to improve process efficiencies in horticultural industries, which are dependent on rapid large-scale vegetative propagation.
... The N content in organic fertilizers comes from organic compost that is degraded by microorganisms, so that the ongoing degradation process (composting) greatly affects the N content in fertilizers [14]. The N content of Aloe vera is 81 mg/100ml f.w [15]. ...
... There is an insignificant difference in K content between the types of animal manures used during the fertilizer manufacturing process [21]. Potassium content of Aloe vera is 61 mg/100ml f.w [15]. ...
Article
Full-text available
The manufacture of organic fertilizers can be accelerated by adding indigenous microorganisms (IMO) which function as decomposers. The purpose of this study was to determine the effect of adding several types of IMO to the N, P, and K content of organic cow manure. This research used animal MOL, namely cow manure IMO and vegetable IMO, namely elephant grass IMO, Aloe vera IMO, pineapple skin IMO and dry cow manures. This study used an experimental method with a completely randomized design with five treatments and four replications. The treatments were A: addition of cow manure IMO, B : elephant grass IMO, C : Aloe vera IMO, D : pineapple skin IMO, E : combination IMO (A, B, C, D). The parameters were measured; the N, P and K content. The results showed that the addition of several types of IMO had a significant effect (P<0.05) on phosphorus content of organic cow manure but had no significant effect (P>0.05) on nitrogen and potassium content. The manufacture of organic cow manure with the addition of several types of IMO for 21 days gave the best results in the combination IMO treatment with 1.70% nitrogen, 0.65% phosphorus, and 1.78% potassium content.
... Así mismo, se observa que una sola célula vegetal (célula meristemática) que sea estimulada con un regulador de crecimiento específico puede llegar a proliferar y diferenciar en un tipo de órgano especializado, el cual puede convertirse en un vástago vegetal, una hoja e incluso una raíz, dependiendo de cuál sea el estímulo aplicado (George, Hall y Klerk, 2008a;Morales, Espinosa y Garza, 2016;Radice, 2010). Otros estudios han comprobado la capacidad productiva que las células vegetales poseen en el inicio de su desarrollo para sintetizar de manera natural algunos reguladores de crecimiento vegetal (Hosny, Hammad, Sharbasy y Zayed, 2016;Sherif, 2017). Los reguladores de crecimiento se clasifican según el efecto promotor o inhibidor de acuerdo con la respuesta que se genera al ser aplicados en tejidos vegetales de manera In vitro y Ex vitro (absición, proliferación, senescencia) (Garay, de la Paz, García, Álvarez y Gutiérrez, 2014;Hussain et al., 2012;Sajjad, Jaskani, Asif y Qasim, 2017). ...
... Existen estudios que comprueban que diferentes organismos pluricelulares y unicelulares pueden producir sustancias que inducen y promueven el crecimiento vegetal (Hussain et al., 2012;Meena, Maurya y Verma, 2014;Mendoza, 2013;Sherif, 2017), por lo que se han aprovechado tres tipos de organismos para el aislamiento de sustancias que estimulen la diferenciación y desarrollo celular. Estos tres organismos son las plantas, bacterias del suelo rizosférico y micorrizas (Calvo et al., 2014). ...
Article
Full-text available
Existen reguladores de crecimiento o fitohormonas que son utilizadas en los cultivos de especies vegetales In vitro /Ex vitro; actualmente se han descubierto más de 10 grupos de compuestos fitohormonales que se utilizan para acelerar y controlar de manera específica el crecimiento de los organismos vegetales o algunos de sus tejidos. Así mismo, se han estudiado diferentes fuentes de metabolitos con potencial fitohormonal sintéticos y orgánicos, con el fin de ser utilizadas en diferentes procesos biotecnológicos. La búsqueda de nuevas fuentes metabólicas para la producción de reguladores de crecimiento permite mejorar la calidad de la producción de plantas, disminuir costos de producción y obtener mejores productos derivados de ellas como los metabolitos secundarios; el objetivo de esta revisión fue describir el uso de algunas fuentes biológicas de origen vegetal como las lentejas, el agua de coco, el Aloe vera y la miel, además, fuentes de origen microbiológico como las rizobacterias, micorrizas y enterobacterias con capacidad de producción de metabolitos con potencial fitohormonal. Estas fuentes pueden ser utilizadas en diferentes procesos de biotecnología vegetal. También se revisaron algunos microrganismos que pueden actuar como bioestimulantes (bacterias y micorrizas) que tienen potencial para mejorar el crecimiento vegetal. El uso de estas nuevas fuentes metabólicas permitirá facilitar el proceso de producción vegetal In vitroy/o Ex vitro,buscando de esta manera técnicas novedosas que permitan reemplazar los reguladores empleados comúnmente (ácido naftalen-acético; 2,4-D; ácido indol butírico, etc.), que suelen ser costosos y tóxicos para el ambiente por las técnicas utilizadas para su producción
... Natural root promoting substances are a costless and safe to use alternative for rooting of horticulture crops. They are environmental friendly and can be used as a substitute for synthetic plant growth hormones like IBA. (El-Sherif et al., 2017). Natural rooting hormone like vermin wash, Coconut water, willow leaf water, honey, humic acid, Seaweed extract, Aloe Vera, cinnamon powder provide a cheap and economically sound mean for propagation and improving the quantity of rooting. ...
... This is due to combined effect of IAA and minerals present in different rooting substances. There is report that aloe vera gel has IAA and it could be used as an alternative hormone (El-Sherif, 2017). Aloe vera gel contains growth hormones like gibberellin and salicylic acid (Sahu et al., 2013) which promotes growth of the plant. ...
... The evaluation units of different treatments types of sugars regarding some measured l date palm syrup; C= 40 g-l glucose; D= 10 g-l Sucrose+ 30 g palm syrup 3.9 succeeded by sucrose treatment 3.5, leats mean value was derived from sucrose + glucose with insignificant differs between glucose treatment, when the plantlets were acclimatized ifferent survival percentage according to different in-vitro l date palm syrup induced 94 l sucrose that gave l sucrose + glucose l sucrose + glucose, the superiority units was recorded under date palm syrup 90.2% followed by sucrose 89.5 %, negligible mean value of survival percentage 69.2 related to glucose treatment, previous results Date palm syrup attain the most evaluated units (Fig. 7) of plant heights leaves numbers and survival percentage of acclimatized plantlets followed by sucrose treatment, slight units contributed to glucose treatment for plant height, and survival percentage, while minimum rates of units of leaves numbers recorded by sucrose + glucose, in this respect Khorsha et al. [47]; Mondal et al. [48]; Cheong and Chanhoon Narayanasamy [50]; Gabryszewska [51]; and Azlan [52]; Rassimwaï et al. [53]; El Sherif [54] and Wojtania and Matysiak [55]. ...
Article
palm (Phoenix dactylifera L.) micropropagation is assessed for its success by its ability to produce plants that have been successfully acclimatized. Shoots of Bartamuda cv. (dry cultivar) resulted from somatic embryogenesis which were cultured on MS medium supplemented with sugars as the main source of carbohydrates from establishment to rooting stage for induce root growth development with high quality of root system formation to generate a suitable plantlet for the success of acclimatization stage. This study aimed to use different various sugars types in MS medium to test the possibility of using it as a substitute for sugar as date palm syrub extract that have highest carbohydtrates percentage as well as nutrients, palm syrup extract prepared from unused fruits (shees that not pollinated) or glucose, upon this, the different treatments were used, i.e. sucrose 30g-l as a control treatment, glucose at 40 and 50 g -l, and date palm syrup extract at 10,20 and 30 ml-l. Throughout the experiment vegetative growth and leaves chemical contents were recorded, the best rooting response was obtained with20 ml-l date palm syrup and 40 g-l sucrose were produced highest plants and roots, increasing leaves and somatic embryogenesis numbers, leaves fresh and dry weights (g), whereas the medium containing 30 g-l sucrose and 10 ml-l date palm syrup successfully increased roots numbers, In addition, the plantlets which grown from the medium supplemented with 40 g-l sucrose or 20 ml-l date palm syrup have the superiority survival percentage of acclimatization stage, tallest plants and great numbers of leaves, also plantlets were grown on the medium includes 30 g-l sucrose and 20 ml-l date palm syrup possess highest leaves contents of chlorophyll a and indoles mg/g, moreover the highest levels of sucrose, date palm syrup and glucose for all levels haven’t induce effects on all tested estimations. Based on these results, date Date palm syrup can be used in the micropropagation technique as a sugars substitute and carbohydrates source which led to increasing benefits of the unused fruits that are not pollinated as the source of income from date palm yield.
... In the past decade, numerous elicitation researches to improve various phytochemicals components of different plant species have been conducted utilizing both biotic elicitors such as leaves extract from Moringa oleifera or Aloe vera or whole cell extract from Spirulina platensis (Ahmad et al., 2014;Amin et al., 2009;El-Shayeb, 2009;El Sherif, 2017;Godlewska et al., 2019) and abiotic elicitors such as NaCl (El Sherif et al., 2013;Petridis et al., 2012;Valifard et al., 2014). In the present study, we have reported the effects of both biotic and abiotic elicitors on the growth, multiplication as well as essential oil compositions of Lavandula officinalis in vitro plantlets. ...
Article
Biotic and abiotic elicitors have been reported to promote growth or multiplication as well as alter bioactive compositions of different plant species. Lavandula officinalis is a perennial plant, which produces fragrant flowers that are utilized to prepare the lavender oil. Lavender oil is rich in beta-linalool, cineol, camphor and many other bioactive compounds, which together contributes to its pain relieving, antimicrobial and cicatrizing activities. Micropropagation is a good alternative to propagate lavender as it reduces the time required to achieve the production of essential oil and eliminates the exposures of the lavender plants to environmental pollutants, thus ensuring a better quality of the oil produced. In this study, we have investigated the effects of 3 biotic elicitors - Moringa oleifera leaf extract (MLE), Aloe vera leaf extract (ALE), Spirulina platensis aqueous extract (SAE) and an abiotic elicitor - Sodium chloride (NaCl) on the growth, multiplication and phytochemical compositions of Lavandula officinalis in-vitro plantlets. All tested biotic elicitors (MLE, ALE, SAE) negatively affected the plantlets fresh weight and shoots height. However, these elicitors were found to enhance the number of leaves per explant. On the other hand, the effect of abiotic elicitor (NaCl) were opposite to that of the biotic elicitors. In contrast to the negative growth effects, SAE and high ALE concentration (40 ml/L) were found to enhance number of shoots per explant. Interestingly, we found that all tested biotic and abiotic elicitors were able to increase the beta-linalool contents in the L. officinalis in-vitro plantlets. We observed that SAE is the best elicitor among the 4 tested elicitors because both 0.5 and 1.0 g/L SAE were able to increase linalool production and enhanced shoots proliferation.
... Відомо також, що сік Aloe vera позитивно впливає на рослинний організм -стимулює проростання насіння, процеси ризогенезу, нарощування вегетативної біомаси, регенерацію рослинних клітин, запобігає швидкому старінню, а також пригнічує ріст бактерій [11,12,15]. ...
Article
Full-text available
Мета. Метою роботи було з’ясувати вплив екстракту Aloe vera на ефективність введення в культуру in vitro та морфогенетичні реакції за умов мікроклонального розмноження представників декоративних сукулентів. Методи. В роботі використовували стандартні біотехнологічні методи мікроклонального розмноження шляхом індукції адвентивного гемогенезу. Вихідний матеріал відбирали з рослин-донорів декоративних сукулентів – Crassula ovata “Hobbit” (Mill), Kalanchoe blossfeldiana (Poelln)., Hatiora salicornioides і Schlumbergera truncata. Як експланти використовували висічки зрілих листків, які після поверхневої поетапної стерилізації культивували на регенераційному середовищі Мурасиге-Скуга (МС) з додаванням синтетичних регуляторів росту – 6-бензиламінопурину (БАП) цитокінінового типу дії та α-нафтилоцтової кислоти (НОК) ауксинової дії. В контрольному варіанті регенераційне середовище (РС) за складом містило половинний набір макро- та мікроелементів та синтетичні фітогормони – ½ МС + 3,0 мг/л БАП + 0,5 мг/л НОК, а в дослідному варіанті в живильне середовище вносили екстракт Aloe vera у співвідношенні 1:100. Досліджували ефективність стерилізації та утворення мериклонів за введення в культуру in vitro та швидкість і ефективність різноманітних морфогенетичних реакцій: калусогенез, гемогенез та ризогенез. Результати. За результатами досліджень встановлено, що екстракт Aloe vera у складі живильного середовища не впливає на ефективність стерилізації листкових експлантів у досліджуваних представників декоративних сукулентів. Морфогенетичний потенціал листкових експлантів реалізується шляхом розвитку процесів калусогенезу протягом першого тижня культивування та інтенсивного адвентивного гемогенезу через 6–8 тижнів культивування. Інтенсивний ризогенез показаний тільки для листкових експлантів – Schlumbergera truncata. Екстракт Aloe vera у складі живильного середовища стимулює ефективність процесу гемогенезу, в 1,3–2,3 рази збільшує кількість мериклонів на одному експланті та на 7–8 діб прискорює швидкість процесу. Досліджувані декоративні сукуленти різняться за ефективністю мікроклонального розмноження – максимальні показники у Crassula ovata “Hobbit” (Mill) та Hatiora salicornioides, мінімальні – у Kalanchoe blossfeldiana. Висновки. Екстракт Aloe vera у складі живильного середовища для мікроклонального розмноження декоративних сукулентів проявляє стимулювальний ефект і може бути запропонований як ефективний біостимулятор для збільшення коефіцієнту розмноження досліджуваних культур.
Article
Full-text available
The objectives of this study was to study the guidelines for promotion and development of fighting fish culture: a case study of Siamese fighting fish farmers group, Bang Muang Sub district, Mueang district, Nakhon Sawan Province. A set of questionnaires was used for data collection administered to a population of 55 farmers. Obtained data were analyzed by using descriptive statistics, as performed by statistical software were package , statistically analyzed using frequency distribution, percentage, mean and standard deviation. The results showed that the average age of famers were 64.45 years old. The average household labor used in the fish culture was 2.39 persons. The average monthly household income was 36,189 Baht. The average culture area was 532.14 m2 which included concrete pond 12.78 ponds and plastic buckets, average 1,424 buckets. The results revealed that frequent problems included lack and/or discontinuity of support from the government, unknown causes of fish death, fish diseases and undersold price. The need for promotion and development of Fighting fish culture at a high level was prevention and treatment of disease. There was individual promotion and development of Fighting fish culture, and the officials in government should have visited their farm and given them suggestions regularly.
Article
Full-text available
Dragon fruit is an emerging fruit in Nepal. The covered area of this fruit has been expanding during the last few years. Vegetative propagation through stem cutting is the easiest and cheapest method for large-scale propagation to produce genetically identical plants. Synthetic rooting hormones have proven to be effective, but their high cost limits their use. Therefore, more research in vegetative propagation using natural substances is needed. The purpose of this study was to investigate how natural substances and synthetic hormones affect the rooting and vegetative growth of dragon fruit stem cuttings in Khumaltar, Lalitpur. The experiment was developed from September 4, 2020 to January 19, 2021. Three treatments were evaluated in a completely randomized design (CRD) with five replications: i) no hormone application (control), (ii) Aloe vera gel (1 mL cutting-1), and (iii) indole-3-butyric acid (IBA) powder (0.5 g cutting-1). Root and shoot growth were evaluated. The results showed that the aloe vera treatment produced the highest number of shoots (2.2) and roots (9.9), similarly to the IBA treatment. The aloe vera treatment considerably increased shoots (30.35 cm) and roots (25.92 cm) length, coinciding with an increase in root (4.55 g) and shoot (102.6 g) weights compared to the other treatments. However, there were no significant differences in shoot diameter in all the treatments. Finally, natural substances that are locally available, such as Aloe vera gel, could be used as an efficient root promoting substance for dragon fruit cuttings.
Article
Full-text available
Abstract—Rooting in hardwood cuttings of olive was investigated at the College of Agricultural Engineering Sciences, University of Sulaimani, Kurdistan Region-Iraq under application of licorice and willow extracts with concentrations of (0, 3, 6 and 9 g.L-1). The extracts were prepared from licorice root and willow shoots in 25% ethanol heated in a water bath at 40ºC for 3 hours, refrigerated for 24 hours and filtered through filter paper. The hardwood cuttings of olive cv. (Sorani) were taken from basal part of one-year-old suckers then soaked in the licorice and willow extract concentrations for 1 hour. The results revealed that the highest (66.66%) rooting was achieved in the cuttings soaked in 6 and 9 g.L-1 licorice extract for 1 hour, they were not different in comparison with control cuttings which gave (49.99%), but rooting percentage was reduced (38.8%) in the cuttings soaked in 6 g.L-1 willow extract. The cuttings soaked in 9 g.L-1 willow extract and 6 g.L-1 licorice extract gave the best root number, root length, shoot length, shoot diameter and leaf number. Generally, depending on the obtained results in this study, 6 and 9 g.L-1 licorice extract with 9 g.L-1 willow extract have possibility to use as an alternative to induce root formation and improve root and shoot quality of the cuttings in olive.
Article
Full-text available
Application of synthetic rooting hormone has been recognized successful but expensive, so need to improve the knowledge of vegetative propagation with using natural substances. This experiment was conducted to study the effect of natural substances and synthetic hormone on rooting and vegetative growth of grapevine cutting. The study comprised of six treatments: (i) no hormone application (control), (ii) Aloe vera gel, (iii) Cinnamon powder, (iv) Undiluted honey, (v) Indole-3-Butyric acid (IBA) and (vi) Indole Acetic acid (IAA). Data on survival ability; vegetative growth and rooting were taken. The results showed that the highest survival percentages and root number observed for synthetic hormone (IBA) which was close to natural substance (Aloe vera gel) treatment. On the other, Aloe vera gel treatment significantly higher than other treatments in terms of vine length, vine diameter, root length and SPAD value. Therefore, natural substances like aloe vera gel treatment could be the effective root hormone for rooting of grapevine cutting.
Article
Full-text available
U radu je istražen alelopatski utjecaj vrste Aloe vera (L.) Burm.f. na klijavost sjemena i rast klijanaca sedam biljnih vrsta. Pokus je proveden u Petrijevim zdjelicama, gdje su procijenjeni ekstrakti svježih listova vrste A. vera u različitim koncentracijama (2, 4, 6, 8 i 10%) na klijavost, duljinu klijanaca te svježu i suhu masu klijanaca ječma, pšenice, soje, uljne bundeve, radiča, rukole i salate. Djelovanje vodenih ekstrakata ovisilo je o koncentraciji ekstrakata i test vrsti. Ekstrakti koncentracije iznad 2% smanjili su klijavost sjemena ječma i salate, dok je pozitivan utjecaj zabilježen kod uljne bundeve. Najveće smanjenje duljine korijena bilo je kod salate i to za 41,6%, dok je duljina korijena uljne bundeve bila povećana do 49,9%. Vodeni ekstrakti povećali su značajno duljinu izdanka kod pšenice, soje i uljne bundeve. Pozitivan i negativan utjecaj utvrđen je na svježu masu klijanaca test vrsta, dok je suha masa klijanaca bila snižena samo kod ječma u svim tretmanima te salate u tretmanu s ekstraktom koncentracije 4%. Među test vrstama, kao najosjetljivija se pokazao ječam kod svih mjerenih parametara, izuzev kod duljine korijena.
Article
Full-text available
T Improving Basil Growth, Yield and Oil Production by Aloe Vera Extract and Active Dry Yeast A.M.A. Hamouda, Dina M.G. Hendi and Omneya F. Abu El-Leel Medicinal and Aromatic Plant Research Department, Horticulture Research Institute, Agricultural Research Centre, Cairo, Egypt. HIS study was conducted during the two successive seasons of …...2010 and 2011 in private farm in Ghetta village, Sharkia Governorate, Egypt. The objective of this research was to study the possibility of using the natural extract of Aloe vera and yeast (which contain macro and micro nutrients, amino acid, plant growth hormones, vitamins and allelochemicals) for improving and stimulating plant growth as well as active ingredients instead of chemicals. Aloe vera extract was used at rates of 0.0, 50, 100 and 200 ml/l while, active dry yeast concentrations were sprayed at rates of 0.0, 10, 20 and 30 gm/l. The plants were sprayed 20 days after transplanting (20 May) and repeated 10 days after each cut, in (20 June) and (20 July) in the two seasons. Foliar spraying with Aloe vera extract and active dry yeast influenced the vegetative growth and oil content of sweet basil (Ocimum basilicum, L.) Generally, the obtained results demonstrated that, spraying basil plants with Aloe vera extract, active dry yeast and its combinations resulted in a stimulation in growth characters in term of plant elongation, branching and fresh herb as well as the biomass of leaves and stems and dry matter production. These results, it could be recommended that, treating basil plant with 200 ml/l aloe extract + 30gm/l active dry yeast or 200 ml/l aloe extract + 20 gm/l active dry yeast produced the highest growth, biomass and the highest oil percent and yield as well as improving oil quality by decreasing estragole (methyl chavicol) content in the basil oil.
Article
Full-text available
The purpose of the present study was to establish a regeneration procedure for Populus × euramericana ‘Neva’ by using in vitro shoots tips and leaves. For sterilization, 0.1% (w/v) mercuric chloride (HgCl2) solution for 8 to 10 min was the optimal treatment for this poplar cultivation. The effects of benzyladenine (BA) and α-naphthaleneacetic acid (NAA) added to Murashige and Skoog (MS) medium were tested on organogenesis. The highest regeneration rate and numbers of shoots/explant from shoot tips (96.7%, 9.8) and leaves (90.0%, 8.7) were obtained on the half-strength MS medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA. The optimal medium for in vitro rooting of shoots was on a half-strength MS medium containing 1 mg/L indolebutyric acid (IBA) with the highest rooting frequency (93.3%) and numbers of roots/explant (8.2). For acclimatization, in vitro rooted plantlets were transferred to plastic cups containing vermiculite and peat (1: 1). After acclimatization, transplanted plantlets grew well in a shade house. Therefore, we believe that this efficient plant regeneration protocol especially by leaf explants is very important for in vitro clonal propagation of Populus × euramericana ‘Neva’.
Article
Full-text available
Aim: The aim was to study the effect of Aloe vera and clove powder supplementation on carcass characteristics, composition and serum enzymes of Japanese quails. Materials and methods: The study was conducted on 120-day-old Japanese quails, which were randomly divided into four treatment groups viz. T1-control, T2-0.5% A. vera, T3-0.5% clove, and T4-(0.25% A. vera + 0.25% clove) powder, each having three replications consisting of ten Japanese quails. The birds in control group (T1) were fed no supplement whereas in treatments T2-T4 birds were supplemented with A. vera leaf powder, clove, and mixture of both (powdered form) at 0.5, 0.5, and 0.25+0.25% of feed, respectively. After 7 days of brooding, a feeding trial of 35 days was conducted. On 35(th) day of trial birds were sacrificed and dressing percentage, cut up parts, organ weight, processing losses and carcass meat composition of the breast, thigh, and drumstick were recorded. Blood samples were collected on the same day and serum was separated to evaluate serum enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Results: It showed significantly (p<0.05) higher values of dressing percentage with and without giblet and breast weight in the T2-T4 group as compared to the T1 group. No significant (p>0.05) differences were observed in giblet weight, other cut up parts and composition of the breast, thigh, and drumstick muscles in all the treatment groups. Serum ALT and AST did not vary significantly among different treatment groups. Conclusion: A. vera and clove supplementation improved the dressing percentage and breast weight without adversely affecting the meat composition and serum enzymes. Thus, these can be used as a growth promoter in Japanese quails.
Article
Full-text available
Among other in vitro factors like temperature and light, concentration of plant growth regulators and medium constituents are two of the most important aspects of successful micropropagation. With the aim of optimization of in vitro multiplication of Populus alba L., Populus tremula L. and Populus tremula L. x Populus. tremuloides Michx , the effect of MS and WPM media with various concentrations of BAP and 2iP was studied. The following multiplication parameters were monitored: number of shoots regenerated/explant, explant height, and explants weight were determined. MS medium proved to be the most effective one, resulting in better and morphologically superior microshoots as compared to WPM medium in the case of Populus alba. However in Populus tremula and Populus tremula x Populus Tremuloides the highest number of shoots was found when grown on WPM medium. In all three poplar lines, the highest shoot multiplication was obtained on MS and WPM media supplemented with BAP at (0.1 and 0.2 mgl -1). Very poor multiplication was achieved on media with 2iP. Shoot tips were isolated and induced to root on MS medium supplemented with IAA, IBA and/or NAA (0.0, 0.1, 0.2 and 0.4 mg l -1). About 90% of the rooted plantlets tested have successfully established in soil. In vitro derived plants were genetically analyzed using RAPD fingerprints. RAPD analysis confirmed that all the in vitro derived plant which tested were genetically identical to their donor plants, suggesting the absence of detectable genetic variation in the regenerated plants. [Salah Khattab.
Article
Full-text available
Aloe vera is a natural product that is now a day frequently used in the field of cosmetology. Though there are various indications for its use, controlled trials are needed to determine its real efficacy. The aloe vera plant, its properties, mechanism of action and clinical uses are briefly reviewed in this article.
Article
Full-text available
Many of the health benefits associated with Aloe vera have been attributed to the polysaccharides contained in the gel of the leaves. These biological activities include promotion of wound healing, antifungal activity, hypoglycemic or antidiabetic effects antiinflammatory, anticancer, immunomodulatory and gastroprotective properties. While the known biological activities of A. vera will be briefly discussed, it is the aim of this review to further highlight recently discovered effects and applications of the leaf gel. These effects include the potential of whole leaf or inner fillet gel liquid preparations of A. vera to enhance the intestinal absorption and bioavailability of co-administered compounds as well as enhancement of skin permeation. In addition, important pharmaceutical applications such as the use of the dried A. vera gel powder as an excipient in sustained release pharmaceutical dosage forms will be outlined.
Article
Full-text available
Trees are used to produce a variety of wood-based products including timber, pulp and paper. More recently, their use as a source of renewable energy has also been highlighted, as has their value for carbon mitigation within the Kyoto Protocol. Relative to food crops, the domestication of trees has only just begun; the long generation time and complex nature of juvenile and mature growth forms are contributory factors. To accelerate domestication, and to understand further some of the unique processes that occur in woody plants such as dormancy and secondary wood formation, a 'model' tree is needed. Here it is argued that Populus is rapidly becoming accepted as the 'model' woody plant and that such a 'model' tree is necessary to complement the genetic resource being developed in arabidopsis. The genus Populus (poplars, cottonwoods and aspens) contains approx. 30 species of woody plant, all found in the Northern hemisphere and exhibiting some of the fastest growth rates observed in temperate trees. Populus is fulfilling the 'model' role for a number of reasons. First, and most important, is the very recent commitment to sequence the Populus genome, a project initiated in February 2002. This will be the first woody plant to be sequenced. Other reasons include the relatively small genome size (450-550 Mbp) of Populus, the large number of molecular genetic maps and the ease of genetic transformation. Populus may also be propagated vegetatively, making mapping populations immortal and facilitating the production of large amounts of clonal material for experimentation. Hybridization occurs routinely and, in these respects, Populus has many similarities to arabidopsis. However, Populus also differs from arabidopsis in many respects, including being dioecious, which makes selfing and back-cross manipulations impossible. The long time-to-flower is also a limitation, whilst physiological and biochemical experiments are more readily conducted in Populus compared with the small-statured arabidopsis. Recent advances in the development of large expressed sequence tagged collections, microarray analysis and the free distribution of mapping pedigrees for quantitative trait loci analysis secure Populus as the ideal subject for further exploitation by a wide range of scientists including breeders, physiologists, biochemists and molecular biologists. In addition, and in contrast to other model plants, the genus Populus also has genuine commercial value as a tree for timber, plywood, pulp and paper.
Article
Full-text available
The profile of endogenous cytokinins in a genetic tumor line of tobacco, namely, Nicotiana glauca (Grah.) x Nicotiana langsdorffii (Weinm.), following 1 to 10 weeks of growth on solid medium was determined by radioimmunoassay. (3)H-labeled cytokinins of high specific activity were added during tissue extraction to correct for the purification losses. Following subculture (of 4-week-old tissues when their cytokinin content is high) onto fresh medium the total cytokinin content continued to be high during the first week (1470 picomoles per gram fresh weight) when the tissue fresh weight remained essentially unchanged (lag phase). The cytokinin levels then declined by about half in 2- and 3-week-old tissues (626 and 675 picomoles per gram fresh weight, respectively), a period when rapid increase in tissue fresh weight was recorded. Increments of 840% and 2780% over initial fresh weight were obtained in 2- and 3-week-old cultures, respectively. The cytokinin content then increased to initial high levels in 4-week-old tissues (1384 picomoles per gram fresh weight) after which it gradually declined with tissue age. The lowest cytokinin levels (432 picomoles per gram fresh weight) were observed in 10-week-old tissues. Maximal tissue fresh weight (4030% increase over initial fresh weight) was recorded in 5-week-old cultures after which it decreased slowly to 77.5% of the highest tissue fresh weight in 10-week-old cultures. Zeatin appeared to be the dominant endogenous cytokinin in tissues of all ages. Other cytokinins quantified were dihydrozeatin, zeatin riboside, and dihydrozeatin riboside; the values may include contributions from aglucones derived from the hydrolysis of corresponding O-glucosides, since the entire basic fraction was treated with beta-glucosidase before analysis. In addition the levels of isopentenyladenine, isopentenyladenosine, and the nucleotides of zeatin riboside, dihydrozeatin riboside, and isopentenyladenosine were also determined.
Article
Aloe vera belongs to the family Xanthorrhoeaceae (APG III System, 2009) commonly known as Ghrit Kumari, is the oldest medicinal plant ever known and the most applied medicinal plant worldwide. Aloe Vera is used for vigor, wellness and medicinal purposes since rigvedic times. Health benefits of aloe vera include its application in wound healing, treating burns, minimizing frost bite damage, protection against skin damage from x-rays, lung cancer, intestinal problems, Increasing High Density Lipoprotein (HDL), reducing Low Density Lipoprotein (LDL), reducing blood sugar in diabetics, fighting Acquired Immuno Deficiency Syndrome (AIDS), allergies and improving immune system. Phyto-chemistry of aloe vera gel has revealed the presence of more than 200 bioactive chemicals. Aloe Vera gel is extracted from its leaves and appropriate processing techniques are needed for stabilization as well as preparation of the end products. Aloe Vera Gel contains important ingredients including 19 of the 20 amino acids needed by the human body and seven of the eight essential ones that just cannot be made. In industries Aloe Vera is also used to extract liver tonic. The present research article is an effort towards the industrial and ethno-botanical properties of Aloe Vera.
Article
Aloe vera (also known as Aloe barbadensis) is an ornamental and a medicinal plant used in the aloe industry. Allelopathic effects of Aloe vera aqueous leaf extracts were determined on seed germination and initial seedling growth of four crops (lettuce, radish, turnip and rice) and re-rooting ability in rice and sweet potato. Although the extracts did not significantly affect seed germination, but significantly promoted the root length and seedling height in all test crops. The extracts increased the number of roots in lettuce and turnip, but not in radish and rice. Besides, the extracts had significant stimulatory affects on re-rooting ability office and sweet potato seedlings. These results suggest that the aqueous Aloe vera leaf extract could be useful as a natural plant-growth regulator, as it promotes initial seedling growth and rooting in cuttings.
Article
A single solution reagent is described for the determination of phosphorus in sea water. It consists of an acidified solution of ammonium molybdate containing ascorbic acid and a small amount of antimony. This reagent reacts rapidly with phosphate ion yielding a blue-purple compound which contains antimony and phosphorus in a 1:1 atomic ratio. The complex is very stable and obeys Beer's law up to a phosphate concentration of at least 2 μg/ml.The sensitivity of the procedure is comparable with that of the stannous chloride method. The salt error is less than 1 %.
Article
The acclimation of plants to water deficit is the result of many different physiological and biochemical mechanisms. To gain a better understanding of drought stress acclimation and tolerance mechanisms in Populus cathayana Rehder, we carried out an integrated physiological and comparative proteomic analysis on the drought stress responses of two contrasting populations originating from wet and dry regions in western China. The plantlets were subjected to continuous drought stress by withholding soil water content at 25% of field capacity (FC) for 45 days, while the control treatments were kept at 100% FC. Drought stress significantly inhibited plant growth, decreased net photosynthetic rate and stomatal conductance of leaves, increased the relative electrolyte leakage and malondialdehyde (MDA) content, and, at the same time, accumulated soluble sugars and free proline in both populations tested. The population from the dry climate region exhibited stronger tolerance to drought stress compared with the wet climate population. The proteomic analyses resulted in the identification of 40 drought-responsive proteins. The functional categories of these proteins include the regulation of transcription and translation, photosynthesis, cytoskeleton, secondary metabolism, HSPs/chaperones, redox homeostasis and defense response. The results suggest that poplars' tolerance to drought stress relates to the control of reactive oxygen species (ROS) and to osmoprotective capacity. The differential regulation of some drought-responsive proteins, such as HSPs and the enzymes related to redox homeostasis and regulation of secondary metabolism, plays an important role in poplars' tolerance and acclimation to drought stress. In conclusion, acclimation to water deficit involves changes in cellular metabolism and the regulation of gene networks. The present study not only provides new insights into the mechanisms of acclimation and tolerance to drought stress in different poplar populations but also provides clues for improving poplars' drought tolerance through breeding or genetic engineering.
Article
The clear pulp, also known as inner gel, of Aloe vera L. leaf is widely used in various medical, cosmetic and nutraceutical applications. Many beneficial effects of this plant have been attributed to the polysaccharides present in the pulp. However, discrepancies exist regarding the composition of pulp polysaccharide species and an understanding of pulp structure in relation to its chemical composition has been lacking. Thus, we examined pulp structure, isolated structural components and determined their carbohydrate compositions along with analyzing a partially purified pulp-based product (Acemannan hydrogel) used to make Carrisyn hydrogel wound dressing. Light and electron microscopy showed that the pulp consisted of large clear mesophyll cells with a diameter as large as 1000 microm. These cells were composed of cell walls and cell membranes along with a very limited number of degenerated cellular organelles. No intact cellular organelles were found in mesophyll cells. Following disruption of pulp by homogenization, three components were isolated by sequential centrifugation. They were thin clear sheets, microparticles and a viscous liquid gel, which corresponded to cell wall, degenerated cellular organelles and liquid content of mesophyll cells based on morphological and chemical analysis. These three components accounted for 16.2% (+/-3.8), 0.70% (+/-0) and 83.1% of the pulp on a dry weight basis. The carbohydrate composition of each component was distinct; liquid gel contained mannan, microparticles contained galactose-rich polysaccharide(s) and cell walls contained an unusually high level of galacturonic acid (34%, w/w; Gal A). The same three components were also found in Acemannan Hydrogel with mannan as the predominant component. Thus, different pulp structural components are associated with different polysaccharides and thus may potentially be different functionally. These findings may help lay a basis for further studies and development of better controlled processing methods and applications for this well-accepted medicinal plant.
Plant Growth Regulator Handbook
  • H G Cutler
  • B A Schneider
Cutler HG, Schneider BA. Plant Growth Regulator Handbook. LaGrange, GA: Plant Growth Regulator Society of America. 1990.
Significant Pharmacological Activities of Aloe vera plant
  • Chatterjee P
  • C Bodhisattwa
  • N Subhangkar
Chatterjee p, Bodhisattwa C, Subhangkar N. Significant Pharmacological Activities of Aloe vera plant. Min. J. Phar. Med. Sci. 2013; 3: 21-24.
Effect of Aloe vera on some annual plants
  • K P Sahu
  • D D Giri
  • R Singh
  • P Pandey
  • S Gupta
  • A K Shrivastava
  • A Kumar
  • D Pandey
Sahu K P, Giri D D, Singh R, Pandey P, Gupta S, Shrivastava A K, Kumar A, Pandey D K. Effect of Aloe vera on some annual plants Sci. Res. Pharmacology and Pharmacy. 2013; 4: 599-610.
Physiological studies on Oenothera biennis bio-fertilizer and plant extracts
  • N S A El-Shayeb
El-Shayeb N S A. Physiological studies on Oenothera biennis bio-fertilizer and plant extracts. PhD. Thesis, Hort. Dept. Fac. Agric. Benha Univ. 2009.
Efficacy of Aloe vera leaf powder as biostimulant in enhancing the growth and yield of Lady's Finger (Abelmoschus esculentus L.). Research on Crops
  • C K Padmaja
  • B Kowsalya
  • C Seethalakshmi
Padmaja C K, Kowsalya B, Seethalakshmi C. Efficacy of Aloe vera leaf powder as biostimulant in enhancing the growth and yield of Lady's Finger (Abelmoschus esculentus L.). Research on Crops. 2007; 8: 395-397.
Effect of certain medicinal plant extracts on growth, yield and metabolism of some medicinal and aromatic and plants
  • A Mady
Mady A. Effect of certain medicinal plant extracts on growth, yield and metabolism of some medicinal and aromatic and plants. (M. Sc. Thesis) Fac. Science Al-Azher Univ. 2008.
Physiological studies on marjoram plants (Majorana hortensis). M. Sc. Thesis of Ornamental Horticulture Department Faculty of Agriculture at Moshtohor Zagazig University (Banha Branch)
  • Nour El-Din
Nour El-Din T. Physiological studies on marjoram plants (Majorana hortensis). M. Sc. Thesis of Ornamental Horticulture Department Faculty of Agriculture at Moshtohor Zagazig University (Banha Branch). 2005.
Growth response of Male and Female clones of Populus Ciliata "Wall
  • P K Khosla
  • G S Deol
Khosla P K, Deol G S. Growth response of Male and Female clones of Populus Ciliata "Wall." Ex royle to nitrogenfertilizer. Proc. Indian main. Sci. Acad. 1984; 50: 603-606.
Soil and plant analysis for Tree Culture, 3 edn. Oxford and IBM Puplishing Co
  • S A Wilde
  • R B Corey
  • J G Lyer
  • G K Voight
Wilde SA, Corey RB, Lyer JG, Voight GK. Soil and plant analysis for Tree Culture, 3 edn. Oxford and IBM Puplishing Co, New Delhi. 1985.
STATISTICA for Windows (software-system fur Datenanalyse) Version 6
  • Statsoft Inc
Statsoft Inc. STATISTICA for Windows (software-system fur Datenanalyse) Version 6. http:/www.statisoft.com. 2001.
Effect of high Voltage on the resistance of Aloe vera leaves. (IJRTE)
  • F A Sheikh
  • R P P Singh
  • P Lehana
Sheikh F A, Singh R P P, Lehana P. Effect of high Voltage on the resistance of Aloe vera leaves. (IJRTE). 2013; 2: 42-45.