Simple MOSFET-based high-voltage nanosecond pulse circuit

Arizona State University, Phoenix, Arizona, United States
IEEE Transactions on Plasma Science (Impact Factor: 1.1). 11/2004; 32(5):1919 - 1924. DOI: 10.1109/TPS.2004.835966
Source: IEEE Xplore


Using simple but powerful electronics concepts, such as a mass produced Schmitt trigger and integrated MOSFET driver in novel circuit applications, a simple 400-V 75-ns pulse generator (pulser) has been designed, developed, and tested. For a 50-Ω matched load, the pulser produces extremely well-defined, repetitive high-voltage pulses that are free from overshoot and ringing. The width of the pulses is adjustable from 75 ns to 10 ms with the fall times of a few tens of nanoseconds for a negative wave and a repetition period of 1.5 μs with the existing setup. By upgrading to a more complex driver circuit, much lower pulsewidths are possible. Using a 1-4 mm standard commercial cuvette, it is possible to generate electric fields of 4-1 kV/cm with this pulser. The purpose is to try to do electroporation mediated gene therapy on mammalian cells at higher electrical field strengths and submicrosecond or microsecond pulsewidths compared to conventional 200 V/cm and tens of millisecond-duration pulsewidths.

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    • "The common switching techniques include the use of MOSFETs (Alton and Sundararaja, 2004), SCRs and avalanche transistors (W. G Maguson, 1962; Molina et al, 2002, Jankee and Navathe, 2006, Lui Jinyuan et al, 1998). "
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    • "The design emphasis was focused on a compact device that had control over the pulse shape. A similar 400 V nanosecond pulse generator for use with electroporationmediated drug and gene delivery was presented in [10]. In order 0093-9994/$25.00 "
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    ABSTRACT: In this paper, a MOSFET-based pulsed power supply capable of supplying square pulses of up to 3000 V and widths from nanoseconds to milliseconds is presented and used for an investigation into electroporation-mediated delivery of a plasmid DNA molecule into the pathogenic bacterium Escherichia coli O157:H7. It was concluded that increasing the electric field strength and pulse amplitude resulted in an increase in the number of transformants. However, increasing the number of pulses had the effect of reducing the number of transformants. In all the experiments, the number of cells that were inactivated by the exposure to the pulsed electric field were also measured.
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    • "It has been used to insert genes and dyes into mammalian cells. Thus the electroporation system is an important mechanism for cell therapy, genetic therapy and drug delivery [4] [5]. "
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    ABSTRACT: Studies into electroporation have grown rapidly in biotechnology and medicine in recent years. This paper presents the design and construction of a low cost programmable electroporation system for biological applications. The system consists of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The programmable electroporation has been designed, developed and tested. Using a standard commercial electroporation cuvette, it is possible to generate electric fields of 100 to 1000V/cm with programmed pulse lengths of 10μsec to 20msec. The system was evaluated with Hela cells and propidium dye to evaluate transfection rates under a variety of electroporation conditions. Initial results showed that the electroporation system achieved a peak cell transfection efficiency of 48.74% at 600V/cm with pulse lengths of 10 ms.
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