Article

Antimicrobial and phytochemical screening of Trikuta- traditional food of western Rajasthan

Authors:
To read the full-text of this research, you can request a copy directly from the authors.

Abstract

Dried plant products of North west Rajasthan which are cooked as a vegetable known as Trikuta-seeds of Acacia Senegal (L.) Willd., unripe fruits of Capparis deciduas (Forssk.) Edgew. and unripe pods of Prosopis cineraria (L.) Druce were tested against seven clinical isolates including one Gram positive and six Gram negative bacteria using Agar well diffusion method. Methanolic extract of unripe pods of Prosopis cineraria and unripe fruits of Capparis decidua showed excellent antimicrobial activity against all the clinical isolates, where as seeds of Acacia senegal showed inhibition against five bacterial strains. The lowest MIC values were recorded by the unripe pods of Prosopis cineraria in comparison to unripe fruits of Capparis decidua and seeds of Acacia senegal. The Phytochemical analysis showed the presence of tannins, alkaloids, flavonoids and glycosides in the Prosopis cineraria and presence of alkaloids, saponins, glycosides in Capparis deidua whereas tannins, alkaloids, steroids, flavonoids and glycosides was evaluated in seeds of Acacia senegal. The study depicts that dried plant products of western Thar Desert possess medicinal properties. © 2017, National Institute of Science Communication and Information Resources (NISCAIR). All rights reserved.

No full-text available

Request Full-text Paper PDF

To read the full-text of this research,
you can request a copy directly from the authors.

... Senegalia seeds have been reported to contain phenolic acids (principally ellagic and vanillic acid), flavonoids ((epi)catechin and rutin), condensed tannins (830-2060 mg/100 g dm), cardiac glycosides, steroids, alkaloids, (hemolytic) saponins, phytate (1030-1528 mg/100 g dm), trypsin inhibitors (0.14-132 TIU/mg protein), lectins, amines, nonproteinogenic amino acids, and fatty acid epoxides (Babu & Subrahmanyam, 2010;Balogun & Fetuga, 1989;Barton et al., 1993;Evans et al., 1977Evans et al., , 1979Evans et al., , 1985Evans et al., , 1993Guissou et al., 2020;Hapner & Jermyn, 1981;Lee et al., 1977;Loganayaki et al., 2011;Odei-Addo et al., 2014;Prakash et al., 2001;Rajaram & Janardhanan, 1991;Shah et al., 1992;Sharma & Kaur, 2017;Sotelo et al., 1995;Tchinda et al., 2020;Thangavelu et al., 2015Thangavelu et al., , 2017Thangavelu et al., , 2020. Again, as it can be noted, there is limited research and details on the composition of Senegalia seeds' phytochemicals. ...
... Therefore, Senegalia seeds are precooked (shortly boiled, washed, and dried) before selling to facilitate the cooking for consumers. Zamnè and Panchkuta are examples of Senegalia seed foods that have become highly appreciated as cultural and healthpromoting foods in Burkina Faso (Msika et al., 2017;Savadogo et al., 2011;Zongo et al., 2022) and the Marwari community in Rajasthan (Northern India state) (Ram et al., 2014;Sharma & Kaur, 2017), respectively. They are recommended by indigenous peoples in cases of diabetes, chronic gastrointestinal disorders, and cardiovascular diseases (including atherosclerosis and hypertension) (Msika et al., 2017;Ram et al., 2014). ...
... (Drabo et al., 2020), 3.(Welcome & Van Wyk, 2019), 4.(Idu et al., 2010), 5.(Arbonnier, 2000), 6.(Hassan & Hamdy, 2021), 7.(Rajaram & Janardhanan, 1991), 8.(Thangavelu et al., 2020), 9. (Janardhanan et al., 2003, 10.(Hashmat & Hussain, 2013), 11.(Malviya et al., 2017), 12.(Fagg & Stewart, 1994), 13.(Powell, 1994), 14.(Msika et al., 2017), 15.(Irvine, 1952), 16.(Ahmad, 2012), 17.(Mkala et al., 2021), 18.(Ram et al., 2014), 19.(Sharma & Kaur, 2017), 20.(Mishra & Pal, 2010), 21.(Lulekal et al., 2011), 22. (Medeiros et al., 2020, 23.(Teixeira et al., 2013), 24. (Warui et al., 2018), 25. (Sutjaritjai et al., 2019), 26. ...
Article
Acacia s.l. is a newly revised genus whose plant species are quintessential in the pantropical, arid, and hunger-prone areas. They provide multiple ecosystem services and a range of edible products for humans. Their food uses are manifestly in the continuum of their medicinal applications, mirroring candidate materials to develop novel healthy food products. Yet, edible Acacia plant products have been overlooked, underresearched, and poorly disseminated for a very long time. This review inventories comprehensively edible products from native Acacia plants in Africa, America, and Asia, critically discusses their food potential for humans, identifies research gaps, and provides bases for further research. Interestingly, the seeds from Senegalia, the second-largest and refined Acacia segregate, appear to be the most promising food materials and are consequently highlighted.
... Sequence identity of corresponding proteins to A0A022RDQ0 report high similarity implying many characteristics including functional properties correspond between these proteins and A0A022RDQ0. This is also an indication that the five plants that include Mimulus guttatus belong to the same family which agrees with Sharma and Kaur. (2017) who suggested plants should be categorized based on similar features such as phytochemicals. High sequence similarity among the proteins as an insight to similar function is consistent with the work of Esposti. (2002) who correlated a common function to some proteins on the basis of sequence similarity. Vol 5 Issue 11 ...
... (2004) who suggested the strongest exhibit for common descent is comparative sequence analysis. Our findings of common evolutionary origin as an insight into similar function is consistent with the work of Petrov et al. (2000) who informed that the configuration of DNA, RNA and amino acids is conserved across generations implying similar proteins would have the same function in different organisms. Diversions in phylogeny could be attributed to junk DNA including pseudogenes that cause mutation thus, reconstructing ancestral lineages as reported by Petrov et al. (2000). ...
... Our findings of common evolutionary origin as an insight into similar function is consistent with the work of Petrov et al. (2000) who informed that the configuration of DNA, RNA and amino acids is conserved across generations implying similar proteins would have the same function in different organisms. Diversions in phylogeny could be attributed to junk DNA including pseudogenes that cause mutation thus, reconstructing ancestral lineages as reported by Petrov et al. (2000). ...
Article
Full-text available
Abstract: This work seeks to characterise and carry out functional analysis of A0A022RDQ0 protein. Basic Local Alignment Search Tool was used to consider specific similarity hits. Uniprot database was used to check predicted functional similarities and subcellular localization of model proteins. Clustal Omega database was used to obtain phylogenetic tree of all proteins to determine evolutionary relationships. Domain/superfamily/multi-domain hits of model proteins were analysed using NCBI’s conserved domain database. Results report that A0A0B2RQU6, G7L0J5, W9SFC3 and A0A0B0P4Q5 proteins respectively show 85.5%, 84.0%, 84.09% and 82.4% similarities to A0A022RDQ0. The four model proteins are ribonucleoproteins that localise in the ribonucleoprotein complex. Phylogenetic tree report that all proteins have similar evolutionary origin. Domain search indicate that all proteins belong to WD40. High similarity in sequence of A0A022RDQ0 to four model proteins as well as similar function, domain, subcellular localization and evolutionary origin of the various proteins suggests that A0A022RDQ0 functions as a ribonucleoprotein. Keywords: Characterisation, Functional analysis, A0A022RDQ0 protein
... References: 1. (Chowdhury et al., 1983), 2. (Pant et al., 1974), 3. (Rajaram and Janardhanan, 1991), 4. (Yadav and Bharadwaj, 1971), 5. (Sotelo et al., 1995), 6. (Guissou et al., 2020), 7. (Hama-Ba et al., 2017), 8. (Savadogo et al., 2011), 9. (Balogun and Fetuga, 1986, and 10. (Tewari and Rathore, 1973 (Thangavelu et al., 2017(Thangavelu et al., , 2020Tchinda et al., 2020;Charan et al., 2022) have been found reporting the profile of the phenolic compounds (i.e., phenolic acids and flavonoids) in Senegalia seeds. The cardiac glycosides, steroids, alkaloids, and saponins are only qualitatively detected (Lee et al., 1977;Hapner and Jermyn, 1981;Sotelo et al., 1995; Ascorbic acid (C) 6.1 6.9 10-20 a References 1-4 5 6-8 1 1 2, 4 9,10 2015; Sharma and Kaur, 2017), inviting further investigation of their compositions before concluding their implications for Senegalia seed foods. Acacia s.l. ...
... the Marwari community in Rajasthan (Northern India state) (Ram et al., 2014;Sharma and Kaur, 2017;Asati et al., 2021;Charan et al., 2022), respectively. Indigenous people recommend them in cases of diabetes, chronic gastrointestinal disorders, and cardiovascular diseases (including atherosclerosis and hypertension) (Ram et al., 2014;Msika et al., 2017;Charan et al., 2022;Zongo et al., 2022a,b Besides, Senegalia seeds are seldom nibbled fresh during foraging, harvested early as fresh and green vegetables or condiments (e.g., in preparation of Kari, a traditional sauce made with S. macrostachya seeds or Zamnè and Bombax costatum red calyx or Voaga in the Yadce community in Burkina Faso), and milled and used as an ingredient to make mush, cake, bread, or tortillas (Table 1.1). ...
Thesis
People in the arid and semi-arid tropics (i.e., Aridoamerica, outback Australia, South America, Southern Asia, and sub-Saharan Africa) are the poorest and most vulnerable to food insecurity in the world, representing more than 80% of undernourished people globally and one of the major handicaps to the sustainable development agenda. However, the vulnerability to food insecurity in those areas remains quite paradoxical, considering that those areas hold the richest biodiversity for food and the largest arable lands in the world. Suffice it to say, people in the arid and semi-arid tropics still have insufficient knowledge and mastery of their environment. In line with the concerted incentives to document, safeguard, and valorize natural or wild food resources, this PhD provides an unprecedented insight into the food and nutritional potential of Acacia s.l. products and particularly the seeds from the segregate genus Senegalia. Overall, this study may help to improve environmental stewardship in the arid and semi-arid tropics and foster several Sustainable Development Goals, including goal 1 (reduce poverty), 2 (end hunger), 3 (promote health and wellbeing), 12 (ensure sustainable consumption and production patterns), 13 (combat climate change and its impacts), and 15 (preserve ecosystems).
... Presence of flavanoides is also reported in other important species of arid zone like Capparis deciduas (Nazar et al. 2018), Zizyphus spp. (Kapoor and Mishra 2013;Koley et al. 2011), Acacia senegal (Sharma and Kaur 2017), Cordia myxa (Malik and Ahmad 2015;Murthy et al. 2019), Salvadora spp (Ebrahim and Mekonnen 2018) and Azadirachta indica (Al-Jadidi and Hossain 2016). ...
... A qualitative phytochemical analysis showed the presence of alkaloids in leaf and bark extracts of T. undulata, therefore leaf and stem bark extracts can be used in synthesis of herbal as well as synthetic drugs and as antifungal agents for crop protection as well (Parveen and Sharma 2014). Phytochemical analysis of dried fruits of C. decidua and seeds of A. senegal revealed the presence of alkaloids which may be responsible for the antimicrobial properties of these plants (Sharma and Kaur 2017). ...
Article
Full-text available
Long rotation period of trees and complex trading procedures of wood, are major impediments in spread of agroforestry. Thus, alternative for providing early return to farmers along with long term benefits of raising trees on their farmlands are necessary. Generally, ~ 25% of the trees can be used as timber while the other parts viz. bark, leaves, roots, branches etc. have limited use and are often burnt. In process, leakage of C sequestration potential of trees is reduced. But, tree twigs and leaves can be the potential source of bioactive compounds or secondary metabolites especially in arid region. Vegetation in arid regions possesses very special and unique chemicals which help them to adapt to prevailing harsh conditions. Water stress in these vegetation leads to production of high quantity of reactive oxygen species (ROS) which through a series of pathways eventually leads to higher production of certain special secondary compounds. These compounds, in nature improve defense mechanism of trees, but also have immense pharmaceutical, food, cosmeceutical, nutraceutical and agrochemicals value. They are locally used as folklore medicine but their commercial exploitation can provide short term benefits to farmers. Also, unlike bole, which can be exploited only once, these benefits from trees can be exploited many times during their growing period. Therefore, in this article research work on important secondary metabolites produced by major agroforestry systems of arid region of India has been reviewed along with their industrial applications.
... There is an increasing numbers of drug-resistant strains, particularly the acquired multi-drug resistant strains which is becoming rapidly growing concern and leads to serious public health problems worldwide [1]. This is as a result of misuse and over prescription of antibiotics which has affected our ability to treat patients empirically [2,3]. Many bacteria have developed resistance against these common antibiotics through methods such as a reduced in cell wall permeability, efflux pump, and hydrolysis of the β-lactam ring by beta-lactamases. ...
... Key:-O1-12 indicates organisms [1][2][3][4][5][6][7][8][9][10][11][12] This study also agrees in part with the study of Samuel and his co workers regarding the isolated bacteria, but they isolated Enterococcus faecalis in addition to those isolated in this present study from the soil [29]. [22], but argue the literature of Sharga and his other workers that Bacilli species were the predominant soil bacteria in the course of study [31]. ...
Article
Full-text available
Antimicrobial agents or antibiotics are the most significant commercially available and utilized secondary metabolites, which are highly produced by the soil microbes (bacteria and fungi) and found to be effective and broad spectrum. Microbes produce metabolic products (antimicrobial agents) through the process called antibiosis. Majority of the classes of antibiotics used are derivatives of animals (microbes) and floras (plants). But currently, the microbial resistance is at the top gear which requires more effort to come up with novel structure, effectual, toxic free and reasonable cost of new antimicrobial products against microbial infections. In the present study, a trial was made to isolate, identify and characterize the antibiotic producing bacteria from the soil samples collected from different sites of abattoir in Lapai, using the standard microbiological techniques. A total of nine (9) bacterial of both groups (Gram positive and negative) which includes Corynebacterium diphtheriae, Bacillus cereus, Escherichia coli, Enterobacter aerogene, Clostridium specie, Bacillus subtilis, Pseudomonas species, Staphylococcus aureus and Klebsiella pneumoniae were isolated in the course of the study. Pseudomonas species was the most frequently isolated bacteria (33.33%) while the rest of the isolates were 8.33% across the five different sampling sites. These isolates were further screened against some pathogenic microbes viz, Salmonella sp, Klebsiella pneumoniae, Pseudomonas aeruginosa and Candida albicans. Three of the bacterial isolates isolated from the abattoir were found to exhibits antimicrobial activity against two pathogenic bacteria used in this study. This study indicates that some of the soil microorganisms could be an interesting source of antimicrobial bioactive substances.
... Infectious diseases are particularly major challenges to public health, despite tremendous scientific discovery of medicines for their treatment [1]. This is due to increase in resistance to antibiotics by pathogenic microorganisms as a result of misuse and over prescription of antibiotics which has affected our ability to treat patients empirically [2,3]. So in recent years, there has been continuous and urgent need to discover new antimicrobial compounds with diverse chemical structures and novel mechanisms of action because the incidence of new and re-emerging infectious diseases and development of resistance to the antibiotics in current clinical use [3,4]. ...
... This is due to increase in resistance to antibiotics by pathogenic microorganisms as a result of misuse and over prescription of antibiotics which has affected our ability to treat patients empirically [2,3]. So in recent years, there has been continuous and urgent need to discover new antimicrobial compounds with diverse chemical structures and novel mechanisms of action because the incidence of new and re-emerging infectious diseases and development of resistance to the antibiotics in current clinical use [3,4]. However, nature is endowed with providing continuous new biomolecules with novel structures that are developed to interact with biological systems in providing defense against infectious diseases are of paramount important [5]. ...
Article
Full-text available
Introduction: The idea that certain plants had healing potential was known long before human beings discovered the existence of pathogens. Methodology: The crude methanolic leaf extract of Prosopis africana was assayed for antimicrobial potency using Agar-well diffusion technique against Salmonella typhi, Klebsiella pneumoniae, Streptococcus pyogenes, Pseudomonas aeruginosa, Methicillin-Resistant Staphylococcus aureus (MRSA) and Candida albicans. Qualitative phytochemical screening was also carried out. Results: The results of the antimicrobial screening showed antimicrobial potency against the test isolates with various degrees of zone of inhibition which varied between 10 mm – 22 mm. The highest zone was reported against Klebsiella pneumoniae (22 mm), followed by Streptococcus pyogenes and Candida albicans (21 mm), Salmonella typhi (20 mm), MRSA (19 mm) and then Pseudomonas aeruginosa (18 mm). Chloramphenicol and Fluconazole are used as reference standard and their zones of inhibitions ranged from 26 mm – 29 mm. The Minimum Inhibitory Concentration (MIC) of the extract ranged between 12.50 mg/mL – 50.00 mg/mL whilst the Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) of the extract were at 50.00 mg/mL. The result of phytochemical screening revealed the presence of carbohydrates, flavonoids, phenols/tannins, saponins, terpenes, steroids, cardiac glycosides and alkaloids as secondary metabolites. Conclusion: The findings clearly showed that methanolic leaf extract of P. africana has proved its use in folklore as an alternative antimicrobial agent and further research can lead to isolation of a new lead of medical importance.
... A. senegal extracts from various parts have been shown in studies to have pharmacological effects against a variety of human diseases [93][94][95]. It has been noted that some Mycobacteria species can be treated with the bark extract of A. senegal to prevent tuberculosis [98]; the leaf extract showed activity against Pseudomonas aeruginosa using the TLC autobiography method [99]; using the agar well diffusion method, the seed extract was significantly effective against one Gram-positive and six Gram-negative bacteria [100]; the hexane extract of the leaves improved the susceptibility of bacteria to phenol antibiotics [96]; and the stem bark extract was significantly active against pathogenic bacteria of the respiratory tract such as Klebsiella pneumoniae, Streptococcus pneumoniae, Pseudomonas aeroginosa, Staphylococcus aureus and Escherichia coli [101]. The crude methanol extract of the leaves possessed a significantly higher total phenol content as it demonstrated antioxidant activity by scavenging ABTS and DPPH radicals and offered promise as a natural antioxidant treatment for oxidative-stress-related diseases [102]. ...
Article
Full-text available
Underutilized legumes are common crops in developing countries with superior dietary potentials that could be useful sources of protein as well as some phytoconstituents. They are more tolerant of abiotic environmental conditions like drought than the major legumes. This makes them more adapted to harsh soil and climatic conditions, which helps to minimize the pressure brought on by climate change. However, despite their potential, underutilized legumes have been greatly overlooked compared to the major legumes due to supply constraints. Underutilized legumes in the subfamily Caesalpinioideae are better suited for use as animal feeds with little or no value as food for humans, and the extracts and infusions of the different parts of plant species in this subfamily are traditionally used for the treatment of different diseases. In addition, underutilized legumes in this subfamily contain phytoconstituents that are of pharmacological relevance, some of which have been isolated, characterized and evaluated for use in the treatment of a variety of disorders. Therefore, this review describes the medicinal activities of some selected underutilized legumes from five genera in the subfamily Caesalpinioideae as well as their phytoconstituents, which could be exploited as lead compounds for drug discovery.
... These bioactive principles in medicinal plants exposed them to human exploitation and these are basically secondary metabolites with proven significant pharmacological properties, produced during the plant's metabolism as a form of defense mechanism against invasion of pathogens, pests and other foreign bodies (Ning et al., 2009;Akinpelu et al., 2015;Anantaworasakul et al., 2017). The plant secondary metabolites such as flavonoids, terpenoids, alkaloids, reducing sugars, steroids, tannins and cardiac glycosides have been deeply studied on how they are used in modern day medicine in the treatment of diseases such as toothache, wound infections, diarrhea, snakebite, paralysis among others (Sharma and Kaur, 2017). The plants products may possess a new source of antimicrobial agents with possible novel mechanisms of action (Ahmad and Aqil, 2007; Barbour et al., 2004). ...
Article
Full-text available
This study investigated the antibacterial and antioxidant potentials of Chrysophyllum albidum leaf extract against selected enteric bacterial pathogens (Escherichia coli, Klebsiella pneumoniae, Shigella dysenteriae, Salmonella typhi, Proteus mirabilis and Proteus vulgaris). Fresh leaves of Chrysophyllum albidum were shade air-dried and ground into fine powder. Thereafter, the leaves powder was cold extracted using methanol and sterile distilled water in ratio 3:2(v/v). The mixture obtained was concentrated in vacuo using a rotary evaporator and lyophilized. The crude extract was screened for antibacterial, phytochemicals and antioxidant properties. The antibacterial properties were determined using agar well diffusion and agar dilution methods while the antioxidant and phytochemical assay were analyzed using standard methods. The phytochemical screening of the extract revealed the presence of tannins, alkaloids, flavonoids, saponins, steroids, terpenoids, reducing sugar and cardiac glycosides. The zones of inhibition shown by the extract at 10 mg/mL against the bacterial isolates ranged between 10 mm and 22 mm. The highest zone of inhibition (22 mm) was expressed against Escherichia coli at a concentration of 10 mg/mL. The MICs ranged between 1.25 mg/mL and 5mg/mL while MBCs ranged between 2.5 mg/mL and 10 mg/mL. The antioxidant assay of leaf extract showed appreciable antioxidant potential when compared with ascorbic acid used as standard. The leaf extract exhibited percentage of 92.03% at a concentration of 500 µg/mL while ascorbic acid exhibited percentage of 96.54% at the same concentration. This study, therefore showed that leaf extract of Chrysophyllum albidum exhibited significant antibacterial and antioxidant activities against the test isolates.
... The similarity of phytochemicals in the study further confirms the plants as emanating from the same family. This is supported by the work of Sharma and Kaur (2017) that proposed the categorisation of plants are based on the presence of similar phytochemical components. ...
Article
Full-text available
There is little information on the use of phytochemicals for the systematic study of variation and resolving relatedness in Nigerian Gossypium hirsutum species. Thus, there is a need to carry out detailed phytochemical studies of the plant leaves, which could be helpful in ascertaining their diversity and relatedness. The qualitative and quantitative phytochemical screening was conducted on 18 accessions of G. hirsutum using the established laboratory protocols. The findings of this study indicated the presence of tannin, alkaloids, proline, flavonoids, and saponin in all of the accessions that were grouped into two at 17% genetic distance, indicating 83% relatedness. However, phytosterol, terpenoids and glycosides were absent in three of the accessions, which were grouped at 48%. The phytochemical compositions were tannin (1.30-1.09 mg/100 g), flavonoid (1.60-1.16 mg/100 g, terpenoid (0.42-0.35 mg/100 g), alkaloid (1.47-1.11 mg/100 g), saponin (4.86-1.59 mg/100 g), glycoside (1.02-0.88 mg/100 g), proline (1.57-1.0 mg/100 g) and phytosterol (0.36-0.2 mg/100 g). The aim of this research was to determine the diversity and relatedness of G. hirsutum genotypes collected from different locations of Nigeria. In general, this study emphasises the use of plant chemicals for classification purposes.
... Its possess antiviral, antibacterial, antifungal, antihelmintic and anticancer properties [16]. Various parts viz., root [17]; stem bark [18,19]; leaves [20] and Pod [21,22] of P. cineraria exhibited antimicro- bial and antifungal properties. The seed proteins are generally consid- ered as defence proteins that act as precocious defensive system during seed germination and early stages of plant development [23]. ...
Article
An antifungal protein with a molecular mass of 38.6 kDa was isolated from the seed of Prosopis cineraria. The protein was purified using ammonium sulphate precipitation, ion exchange chromatography and gel filtration. The antifungal activity of purified protein was retained up to 50 °C for 10 min. The MALDI TOF mass spectroscopy revealed 15 assorted peptides. The molecular weight of the antifungal protein is different from antifungal proteins reported in seeds of other leguminous plants. The purified protein exerted antifungal activity against post-harvest fruit fungal pathogens Lasiodiplodia theobromae and Aspergillus fumigatus, isolated from the rotten fruits. The antifungal properties of this novel antifungal protein can be potentially exploited to manage post-harvest fungal disease of fruits through alternative means to reduce use of hazardous chemicals.
... show differences in stem and flower colour. This is consistent with the findings of Okezie et al. (1998) suggesting the basis for grouping both plants in different genera. Similar phytochemicals were observed in the plants studied which further support our hypothesis that the two plants belong to the same family. This emanated from the findings of Sharma and Kaur. (2017) who proposed the categorisation of plants based on the presence of similar phytochemical components. Presence of alkaloids, flavonoids, saponins and tannins in the two plants correlates with the findings of Swarna and Ravidhran, (2013) and Arrais Silva et al. (2014) who identified similar chemical components during their studies on rela ...
Research
Full-text available
This work seeks to investigate taxonomic studies of representative plants of Portulacaceae family in Dutsin-Ma Local Government Area. Plant samples were collected for identification using morphological characters and assigned voucher numbers, phytochemical composition of explored species were determined using standard phytochemical screening test. Quantitative analysis to ascertain the taxonomic relationship between family members was done by spectrophotometry and students t-test was employed to check differences at 5%. Results reveals that Talinum triangulare (water leaf) and Portulaca oleracea (ten O’clock plant) are the common members of the Portulacaceae family present in Dustin-Ma. Phytochemical analysis reports the presence of alkaloids, flavonoids, saponins and tannins in both plants. Quantitative phytochemical comparison of Talinum triangulare to Portulaca oleracea respectively reveals alkaloid content to be 1.50>1.10mg/1000mg, flavonoids; 2.04>1.80mg/1000mg, saponins; 2.84<2.96mg/1000mg and tannins; 4.22>4.01mg/1000mg. Statistical test report significant difference (P≤0.05) in phytochemical components between the two plants. Presence of similar phytochemical contents in both plants confirms that they belong to the same family. Significant difference between each phytochemical in the two plants suggests that they belong to the same family but different genera taxonomically. Keywords: Taxonomic classification, family portulacaceae, water leaf, ten O’clock plant.
... show differences in stem and flower colour. This is consistent with the findings of Okezie et al. (1998) suggesting the basis for grouping both plants in different genera. Similar phytochemicals were observed in the plants studied which further support our hypothesis that the two plants belong to the same family. This emanated from the findings of Sharma and Kaur. (2017) who proposed the categorisation of plants based on the presence of similar phytochemical components. Presence of alkaloids, flavonoids, saponins and tannins in the two plants correlates with the findings of Swarna and Ravidhran, (2013) and Arrais Silva et al. (2014) who identified similar chemical components during their studies on rela ...
Article
This work seeks to investigate taxonomic studies of representative plants of Portulacaceae family in Dutsin-Ma Local Government Area. Plant samples were collected for identification using morphological characters and assigned voucher numbers, phytochemical composition of explored species were determined using standard phytochemical screening test. Quantitative analysis to ascertain the taxonomic relationship between family members was done by spectrophotometry and students t-test was employed to check differences at 5%. Results reveals that Talinum triangulare (water leaf) and Portulaca oleracea (ten O’clock plant) are the common members of the Portulacaceae family present in Dustin-Ma. Phytochemical analysis reports the presence of alkaloids, flavonoids, saponins and tannins in both plants. Quantitative phytochemical comparison of Talinum triangulare to Portulaca oleracea respectively reveals alkaloid content to be 1.50>1.10mg/1000mg, flavonoids; 2.04>1.80mg/1000mg, saponins; 2.84<2.96mg/1000mg and tannins; 4.22>4.01mg/1000mg. Statistical test report significant difference (P≤0.05) in phytochemical components between the two plants. Presence of similar phytochemical contents in both plants confirms that they belong to the same family. Significant difference between each phytochemical in the two plants suggests that they belong to the same family but different genera taxonomically. Keywords: Taxonomic classification, family portulacaceae, water leaf, ten O’clock plant.
... Wamburu et al. 15 have reported the spectrometric detection of organic compounds and toxicity of ethanolic leaves extracts of P. Juliflora. Sharma & Kaur 16 have reported that methanolic extracts of unripe pods of Prosopis cineraria, seeds of Acacia senegal and dried fruits of Capparis decidua (Trikuta, traditional food of western Rajasthan) showed antimicrobial activity but in unripe pods of P. cineraria the activity was found more pronounced than in C. decidua's dried fruits and A. senegal seeds when tested against seven clinical isolates including one Gram positive and six Gram negative bacteria by Agar well diffusion method. ...
Article
Full-text available
Prosopis juliflora (Sw.) DC. (Fabaceae) commonly known as 'Mesquite' or 'Honey Mesquite' and Balanites aegyptiaca Del (Zygophyllaceae) or Desert Date are invasive weeds and have been known for debates on their use as well as harm. The present study reports the phytochemical screening, physico-chemical characterization, thermal stability and toxicity test of gum exudates of P. juliflora and B. aegyptiaca, collected from Jodhpur (Rajasthan). Standard qualitative chemical tests have revealed the presence of flavonoids, saponins, amino acid / protein and carbohydrate in both the gum exudates. Physicochemically, they were characterized by determining their moisture content (%), colour parameters (L, a, b), ash content (%), elemental (CHNS) analysis, specific rotation [] in H2O, deg., heavy metals (mg/g), viscosity (cP) and FTIR. Gums were also thermally characterized using differential scanning calorimetry (DSC), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA). Thermal studies have shown that the gums are relatively stable up to 220°C, beyond which degradation starts. Short-term, acute oral toxicity test of both the gum exudates was carried out on albino rats of Wistar strain and found that acute toxicity of both the gum exudates by oral route was >2000 mg/kg body weight and no compound related sign, symptoms and mortality were produced in the animals at this permissible limit dose.
Article
Full-text available
Climate change has posed a challenge for food security all over the world in the form of fluctuating crop yields and novel disease outbreaks in plants. Human society's overdependence on a few food crops does not seem a wise precedence. There are numerous underutilized/orphan/neglected legumes growing in the Indian desert regions that can come to the rescue and act as balanced and sustainable sources of nutrients and health-benefitting nutraceuticals. However, challenges such as low plant yield, unidentified metabolic pathways and off-flavor in the food products derived from them prevent the realization of their full potential. Conventional breeding techniques are too slow to achieve the desired modifications and cater to the sharply rising demand for functional foods. The novel gene editing tools like CRISPR-Cas provide more precise tool to manipulate the target genes with or without introduction of foreign DNA and therefore, have better chances to be accepted by governments and societies. The current article reports some of the relevant 'gene editing' success stories with respect to nutraceutical and flavor profiles in the popular legumes. It highlights gaps and future potential, along with areas requiring caution, in underutilized edible legumes of the Indian (semi) arid regions like Prosopis cineraria, Acacia senegal and Cyamopsis tetragonoloba.
Article
The emergence of new diseases and multiple-antibiotic resistance pathogens that resist the action of clinically used antibiotics have increased the needs of discovering new antibiotics. However, soil served as the most important target for most researchers in their attempt of discovering new antibiotics that have pharmaceutical values. The aim of this study was to detect the antibiotic-producing bacteria from soil samples in parts of Wudil local government of Kano state using standard procedure. The color ranged from light brown to dark brown. The texture ranged from clay loam, clay and loam. Sample C was found to have the highest mean pH and electrical conductivity of 7.46 and 108.90μs/cm, respectively, while sample B had the least mean values of 6.95 and 73.53μs/cm, respectively. The highest mean bulk devsity and particle density was sample B with 1.60g/cm3 and 1.87g/cm3, respectively, while sample C had the least mean values of 1.48g/cm3 and 1.71g/cm3, respectively. Sample C was found to have the highest mean moisture content and porosity with 17.93% and 15.57%, respectively, while sample A had the least mean values with 10.61% and 10.74%, respectively. Four bacterial spp. were identified based on morphological description, gram’s reaction and biochemical tests, and were identified as Bacillus spp., Micrococcus spp., Pseudosomonas spp. and Proteus spp. Sample C was found to have the highest mean total bacterial viable plate count of 19.9×105cfu/g, while sample B had the least mean value of 4.1×105cfu/g. Bacillus spp. was found to have the highest percentage occurrence with 21(47%), while Proteus spp. had the least value with 4(9%). E. coli had the highest and least susceptibility to metabolites produced by Bacillus spp. and Pseudosomonas spp. with inhibition zones of (17 and 0mm) respectively. While the control (Erythromycin 250mg/ml) produced the inhibition zones of (27 and 21mm) against E. coli and S. aureus respectively. It was concluded that soil samples in Lajawa, Kausani, Wudil Sabon gari and Wudil Tsohon gari of Wudil local government contained the bacteria with potentiality of producing antimicrobial substance and recommended the use of molecular techniques for further characterization.
ResearchGate has not been able to resolve any references for this publication.