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Draft Genome Sequence of Multidrug-
Resistant Abortive Campylobacter jejuni
from Northern California
Allison M. Weis,
a
Kristin A. Clothier,
b
Bihua C. Huang,
a
Nguyet Kong,
a
Bart C. Weimer
a
School of Veterinary Medicine, 100K Pathogen Genome Project, UC Davis, Davis, California, USA
a
; California
Animal Health and Food Safety, Davis, California, USA
b
ABSTRACT Campylobacter jejuni is an enteric bacterium that can cause abortion in
livestock. This is the release of a multidrug-resistant Campylobacter jejuni genome
from an isolate that caused an abortion in a cow in northern California. This isolate
is part of the 100K Pathogen Genome Project.
Campylobacter jejuni is an enteric pathogen that affects humans worldwide with
infection accompanied by symptoms such as fever and bloody diarrhea, and can
lead to autoimmune diseases (1–6). C. jejuni is zoonotic, and in livestock it can lead to
gastroenteritis and occasionally abortion (7–12). In recent years, several regions in the
United States have noted the emergence of an abortive hypervirulent C. jejuni isolate
(13–15).
Multidrug resistant bacteria are a major worldwide problem, leading to untreatable
infection and mortality among humans and other animals (16, 17) that can be trans-
mitted to the human microbiome (18). Here, we describe a multidrug-resistant abortive
C. jejuni strain from northern California that was isolated from a bovine fetus from a
third trimester abortion in 2009 and was uniquely multidrug resistant. This C. jejuni
genome contains tetO, which confers resistance to tetracycline and its derivatives. It
also contains aphA, a kanamycin resistance gene that is an aminoglycoside phospho-
transferase. Lastly, it also contains a Thr-86-Ile mutation in the GyrA protein that results
in fluoroquinolone resistance. The tetO and aphA genes are located on a putative
plasmid, whereas gyrA is in the chromosome. The genome assembly comprised 54
contigs, 1,708,778 bp, and 1,717 coding sequences, and the sequence depth was 59⫻.
This C. jejuni isolate was among a set of animal source isolates that was given to the
100K Pathogen Genome Project (http://www.100kgenomes.org) in the laboratory of
Bart Weimer (UC Davis). The isolate was checked for purity (19) and genomic DNA
(gDNA) was extracted from culture grown on 5% blood agar plates (UC Davis, VetMed
Biological Services) for 1 to 2 days, lysed with an enzyme cocktail (20), purified with a
Qiagen QIAamp DNA minikit (51306), and analyzed on the Agilent 2200 TapeStation
system with the Genomic DNA ScreenTape assay for integrity of gDNA, as described
previously (21–23). Approximately one microgram of gDNA was used for library con-
struction with the KAPA Hyper library preparation kit (Kapa Biosystems, Boston, MA,
USA; KK8514), on a PerkinElmer Sciclone NGS Workstation (Waltham, MA, USA). In this
process, fragmented double-stranded gDNA molecules were end-repaired (5=), adeny-
lated (3=), and ligated with double stranded DNA adapters (Weimer 384 TS-LT DNA
Barcodes, Integrated DNA Technologies, Inc., Coralville, IA, USA), followed by dual-SPRI
size selection for targeting a library size of 250 to 500 bp. Final library quality
assessment to verify size distribution was performed on a Perkin Elmer Caliper Lab Chip
GX using the PerkinElmer Caliper HT 1K kit (CLS701879), and library quantification was
completed using a SYBR Green– based qPCR kit and a KAPA library quantification kit
Received 13 February 2017 Accepted 21
February 2017 Published 13 April 2017
Citation Weis AM, Clothier KA, Huang BC, Kong
N, Weimer BC. 2017. Draft genome sequence
of multidrug-resistant abortive Campylobacter
jejuni from northern California. Genome
Announc 5:e00171-17. https://doi.org/10.1128/
genomeA.00171-17.
Copyright © 2017 Weis et al. This is an open-
access article distributed under the terms of
the Creative Commons Attribution 4.0
International license.
Address correspondence to Bart C. Weimer,
bcweimer@ucdavis.edu.
PROKARYOTES
crossm
Volume 5 Issue 15 e00171-17 genomea.asm.org 1
(KK4824). Pooled libraries were sequenced on the Illumina HiSeq X TEN platform with
a PE150-plus index read at Novogene (Sacramento, CA, USA). Paired-end sequence
reads were assembled using ABySS version 1.9.0 (k-mer ⫽64) and annotated with
Prokka (24, 25).
Accession number(s). This sequence can be found at NCBI SRA BioProject
PRJNA186441 under genome SRA accession number SRR5210995, and at NCBI GenBank
under the accession number MUJW00000000.
ACKNOWLEDGMENTS
We thank all contributors of the 100K Pathogen Genome Project and members of
the Weimer Lab for technical assistance. We thank the individuals associated with the
isolation of the unique and important C. jejuni strain presented here. This project was
funded by the 100K Pathogen Genome Project to produce this sequence.
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Weis et al.
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