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OMICS International
Journal of Diabetes & Metabolism
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ISSN: 2155-6156
El-Tahawy et al., J Diabetes Metab 2017, 8:3
DOI: 10.4172/2155-6156.1000730
Volume 8 • Issue 3 • 1000730
J Diabetes Metab, an open access journal
ISSN: 2155-6156
Research Article
Abstract
Introduction: Diabetes mellitus is a global problem and several restoration approaches have been developed
to induce beta (β) cells regeneration. Platelet rich plasma (PRP) is an autogenous and economical source of growth
factors which nowadays used in the tissue repair.
Aim: To test the hypothesis that PRP could play a role in the improvement of the structural changes occurred in
the endocrine pancreas of experimentally- induced diabetic rats and the possible mechanisms through which PRP
induced its effects to shed a light on the possible use of such application in the clinical eld.
Material and methods: Sixty male albino rats were used; 20 for obtaining the PRP and 40 were divided into
4 equal groups (10 rats each): control, PRP-group, diabetic group, PRP/diabetic group. Diabetes was induced by
single intra-peritoneal injection of streptozotocin (50-60 mg/kg). The PRP was administered by SC injections in a
dose of 0.5 mg/kg twice weekly for 3 weeks.
Results: The diabetic group showed a signicant increase in blood glucose levels compared to the control.
Treatment with PRP signicantly reduced the blood glucose levels compared to the diabetic group. The diabetic
group showed variable marked morphological changes which diminished by the PRP administration. PRP/diabetic
group had a signicant increase in the mean number of pancreatic islets and β-cells/islet compared to the diabetic
group. The islet cells appeared normal with scarcely seen vacuolations. The duct system showed several changes;
stratications, invagination of the surface epithelium to the underlying connective tissue, and sprouting of the ductal
epithelial cells in between the lobules. Numerous small islets were noticed in a close association with the intra-
lobular ducts. Small newly lobules with abundant connective tissue were organized. There were signicant increases
in the insulin immunopositive β-cells and PCNA positive cells in PRP/diabetic group compared to diabetic group.
Conclusion: This study provides an evidence of the diabetic pancreatic islet regeneration in response to
PRP treatment. The PRP stimulated islet cell regeneration and stimulated the induction of other sources of β-cells
generation as the exocrine portion of the pancreas; ductal and acinar cells. In addition, PRP might put the pancreas
into an environment similar to the postnatal developmental one where new lobules were formed. These will pave the
future for a novel treatment for diabetes.
Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine
Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A
Histological and Immunohistochemical Study
Nashwa Fathy El-Tahawy1*, Rehab Ahmed Rifaai1, Entesar Ali Saber2, Saadia Ragab Saied1 and Randa Ahmed Ibrahim1
1Department of Histology and Cell Biology, Minia University, Minia, Egypt
2Department of Histology and Cell Biology, Minia University, delegated to Deraya University, New Minia, Egypt
*Corresponding author: Fathy El-Tahawy N, Department of Histology and Cell
Biology, Faculty of Medicine, Minia University, Minia, Egypt, Tel: 0201145435777;
E-mail: nashogo@yahoo.com
Received February 25, 2017; Accepted March 09, 2017; Published March 16,
2017
Citation: El-Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA (2017)
Effect of Platelet Rich Plasma (PRP) Injection on the Endocrine Pancreas of
the Experimentally Induced Diabetes in Male Albino Rats: A Histological and
Immunohistochemical Study. J Diabetes Metab 8: 730. doi: 10.4172/2155-
6156.1000730
Copyright: © 2017 El-Tahawy NF, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original
author and source are credited.
Keywords: Diabetes mellitus; Streptozotocin; Platelet rich plasma;
Rats
Introduction
Diabetes mellitus (DM) is a global problem. It was the direct reason
of 1.5 million deaths in 2012 [1]. It is predicated that the number of
diabetic person in the world could reach up to 366 million by the year
2030 [2]. Type 1 diabetes results from the autoimmune destruction
of pancreatic beta (β) cells of the islets of Langerhans [3]. Exogenous
insulin is an important treatment for type1 diabetes but it is not a
physiological method to regulate the blood glucose levels, as it was not
administered in relation to glucose concentration. Beta cell replacement
therapies using either the pancreas or the islet transplantation were
used as a therapeutic alternative to the administration of exogenous
insulin [4]. However, these procedures associated with many problems
such as risks of major invasive surgery along with side eects of
immunosuppressive therapy [5]. Special alternative ways to generate
β-cells from endogenous sources should be found as a way for the
development of treatment. is is to avoid the complication of tissue
matching and surgical procedures [6]. To date several restoration
approaches have been developed to induce β-cells regeneration through
the induction of the proliferation of remaining β-cells, neo-genesis;
de novo islet formation from pancreatic progenitor cells, and trans-
dierentiation; converting non-β-cells within the pancreas to β-cells.
ese models of induction are the most simple, direct, and least invasive
ways to increase β-cell mass [7].
Streptozotocin (STZ) is a naturally occurring compound. It has
also been used as an antibiotic and cancer treatment. e STZ has
been widely used for inducing experimental diabetes mellitus [8].
Growth factors (GFs) are natural biological mediators that control
Citation: El-Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA (2017) TEffect of Platelet Rich Plasma (PRP) Injection on the Endocrine
Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab
8: 730. doi: 10.4172/2155-6156.1000730
Page 2 of 9
Volume 8 • Issue 3 • 1000730
J Diabetes Metab, an open access journal
ISSN: 2155-6156
growth, dierentiation, and have role in the process of tissue repair and
regeneration [9]. Recently, long-term administration of a low dose of
epidermal growth factor (EGF) induces β-cell neo-genesis in diabetic
mice and induced ductal cell dierentiation into β- cells [10]. Platelets
convey many growth factors (GFs) [11]. Platelet-Rich Plasma (PRP)
is a low-cost procedure to deliver high concentrations of autologous
GFs [12]. PRP has been dened as an autologous concentration of
platelets that is 3 to 5 times greater than physiologic concentration of
thrombocytes in whole blood [13]. PRP therapy represents a relatively
new approach in regenerative medicine and accumulated considerable
attention over the two last decades [14]. It was used in many medical
and surgical elds [15] such as dentistry, orthopedics, neurosurgery,
ophthalmology, maxillofacial surgery, and cosmetic surgery [16].
PRP has been the subject in dierent studies in medicine, but there
was a lack of studies that handling the eect of PRP injection on the
diabetic pancreas. e aim of this was to test the hypothesis that PRP
could play a role in the improvement of the structural changes occurred
in the endocrine pancreas of experimentally-induced diabetic rats and
the possible mechanisms through which PRP induced its eects to shed
a light on the possible use of such application in the clinical eld.
Materials and Methods
Animals
is study was conducted in the Histology Department, Faculty
of Medicine, Minia University and carried on 60 adults male albino
Wistar rats weighing approximately 150-250 gm, of 8-10 weeks which
were pathogenically free. Animals were obtained from the animal house
of faculty of agriculture, Minia University. Rats were housed in clean
plastic cages and fed a standard laboratory diet with free access to water
and diet at room temperature with normal light/dark cycles. All aspects
of animal care and treatment were carried out according to the local
guidelines of the ethical committee of the Faculty of Medicine, Minia
University.
Reagents
• Streptozotocin (STZ): A vial containing 1.5 g powder of STZ
and 220 mg citric acid (Sigma Aldrich, Egypt) which was freshly
dissolved in 0.1 M sodium citrate buer (pH 6) according to
the manufacture instructions and was used within 5 minutes of
preparation.
• Anti-insulin antibody: A monoclonal mouse antibody, ultra-
vision one detection system, HRP Polymer & DAB plus
Chromogen (ermo Fisher Scientic, USA).
• Anti-proliferating cell nuclear antigen (anti-PCNA): A
monoclonal mouse antibody, horseradish peroxidase coupled
to an inert polymer backbone & DAB Plus Chromogen (Dako-
EPOS, USA).
e platelet rich plasma (PRP) was freshly prepared.
Experimental design
Twenty male rats were used for obtaining PRP and the other 40 rats
were randomly divided into 4 equal groups (10 rats each):
• e control group: Rats received i.p injections of citrate buer
saline in a dose of 0.5 ml /kg twice weekly for 3 weeks.
• e PRP-group: Rats received PRP in a dose of 0.5 mL/kg by
subcutaneous injection (SC) injection twice weekly for 3 weeks.
• e diabetic group: Rats received a single i.p injection of STZ
in a dose of 50-60 mg/kg body weight for induction of diabetes.
Aer 48 hours (considered day 0) of STZ injection, animals were
fasted overnight and blood samples were collected from the tail
vein and glucose levels were measured. Individual glucose levels
reached above 250 mg/dl was considered diabetic [16].
• e PRP/diabetic group: Rats received a single i.p injection
of STZ in the same dose as the diabetic group. At day 0; aer
the conrmation of the induction of the diabetes, the PRP was
given in the same dose as PRP-group, twice weekly for 3 weeks.
Procedures
Preparation of PRP: e PRP preparation was performed at the
pharmacology department of faculty of medicine, Minia University.
e PRP preparation was carried out by adapting the protocol of the
double centrifugation tube method [17]. In brief, rats were anesthetized
with ether, 2 ml of blood was collected under aseptic technique from
the retro-orbital plexus using capillary tube initially dipped in 3.2%
sodium citrate, then collected into tubes containing 0.3 mL of the
anticoagulant. e blood was subjected to double centrifugation
method, in the rst centrifugation the tubes were centrifuged at 1600
revolutions per minute (rpm) for 10 minutes. is resulted in 3 dierent
density compartments; the inferior layer contained red blood cells, the
intermediate layer contained buy coat of white blood cells, and the
superior layer contained plasma. e plasma was pipetted and the
portion just above buy coat was obtained without disturbance of the
buy coat. e plasma was centrifuged again at 2000 rpm for 10 minutes.
is resulted in 2 parts: the top consisted of platelet-poor plasma (PPP)
and the bottom consisted of the platelet button. Part of the PPP was
discarded and part was remained in the tube along with platelet button
which then gently agitated to promote platelets resuspension. is
procedure resulted in the production of platelet-rich plasma (PRP). For
conformation of the platelet's concentration, 80 μL of the PRP sample
was counted in an automatic apparatus to verify that the platelet count
was greater than 1,000,000/μL.
e PRP administration: 0.5 ml PRP was dissolved in phosphate
buer saline (PBS) (PRP 1:1 PBS), then immediately aspirated
with micropipette, placed in a sterile insulin syringe, and injected
subcutaneously [18]. Rats were sacriced at the end of 3rd week for all
groups by decapitation under light halothane anesthesia. Pancreas was
rapidly removed and xed.
Biochemical study: Blood glucose levels were detected by the use
of one touch Accu-check glucometer® and compatible blood glucose
test strips [19]. At the beginning of the study, the blood glucose levels
were measured for all animals before grouping them to ensure that
the animals were all normoglycaemic. en blood glucose levels were
measured for the dierent study groups and measures were statistically
analyzed.
Histological study: Pancreatic specimens were xed in 10% buered
formalin for 24 hours, dehydrated in a graded alcohol series, cleared in
xylene, and embedded in paran. 5 μ-sections were mounted on glass
slides for further staining with haematoxylin and eosin (H&E) and
other sections with Masson trichrome according to Bancro et al. [20].
Immunohistochemical study: Other 5 μ-sections were used for
immunestaining. Briey [21] sections were deparanized in xylene,
rehydrated in descending grades of alcohol and immersed in 0.1%
hydrogen peroxide for 15 minutes to block the endogenous peroxidase
activity. en sections washed by phosphate buer, followed by
Citation: El-Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA (2017) TEffect of Platelet Rich Plasma (PRP) Injection on the Endocrine
Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab
8: 730. doi: 10.4172/2155-6156.1000730
Page 3 of 9
Volume 8 • Issue 3 • 1000730
J Diabetes Metab, an open access journal
ISSN: 2155-6156
incubation in the ultravision block for 5 minutes at room temperature
to block the non-specic background staining. e primary antibody
(anti-insulin antibody) was diluted at 1:200 in antibody diluent while
the anti-PCNA antibody was ready to use. Sections were incubated in
the primary anti-insulin antibody for 30 minutes while incubated for 60
minutes with anti-PCNA antibody at room temperature. e reaction
was visualized using; Ultravsion one detection System, HRP Polymer
& diaminiobenzide (DAB) Plus Chromogen. Aer completion of the
reaction, counter staining was done using hematoxylin. Sections were
dehydrated by ascending alcohol concentrations, cleared by xylene, and
mounted. Positive cells for the anti-insulin antibody showed brown
cytoplasmic reaction and positive cells for the anti-PCNA antibody
showed brown nuclear reaction.
e positive control for anti-insulin was the normal pancreatic
tissue of the control animals while positive control for the anti-PCNA
antibody was the sections from colon of the control animals. For
negative control slides, the same steps were applied but the 1ry antibody
was not added to the pancreatic sections from the control group (gures
not included).
D-Morphometrical analysis: ree sections were examined from
each animal in the dierent groups. e morphomertrical studies were
made using Leica Qwin 500 Image Analyzer computer system (Leica
Microsystem Imaging Solution Ltd., Cambridge, UK).
Quantitative data were collected for 3 parameters
• e number of islets per square millimeter of each section
• e islets of the pancreas were counted in H&E slides randomly
under 10 power elds. e number of islets was assessed by
counting all islets per one square millimeter.
• e number of β-cells in the islet
• e β-cells of the pancreas were counted in the anti-insulin
immunostained sections under 40 power elds. e number of
β-cells was assessed by counting the nuclei of all positive cells
inside one islet in the eld. A total number of 30 islets for each
group were counted.
• e number of PCNA positive cells in the islet
• e PCNA positive cells in the islet of pancreas were counted in
the anti-PCNA immunostained sections under 40 power elds.
e brown nuclei inside one islet in the eld were counted. A
total number of 30 islets were counted for each group.
Statistical analysis: Quantitative data was analyzed by SPSS (IBM
Corp. Released 2010. Windows, Version 19.0). e mean and standard
deviation (sd) was calculated for the parameters of each group. Values
were expressed as means ± sd. One-way analysis of variance (ANOVA)
test was used for the detection of signicant dierences between groups,
followed by the use of Tukey-Kramar as a post hoc test. e results were
considered statistically signicant when the p-values were <0.05.
Results
Biochemical results
e PRP-group showed normal blood glucose levels with no
signicant dierence compared to the control group (p=0.900). ere
was a signicant increase in blood glucose levels aer STZ injection in
the diabetic group compared to the control group (p=0.0001). ere
was a signicant decrease in the blood glucose levels in the PRP/diabetic
group compared to the diabetic group (p=0.0001), but it showed a
signicant increase if compared to the control group (p=0.046).
Histological results
H&E and Masson trichrome stains results: H&E stained sections
from the control group (Figure 1a and 1b), showed normal histological
structure of the pancreatic tissue that was formed of lobules packed
with acini and separated from each other by a delicate connective
tissue. e islets of Langerhans appeared as pale stained rounded or
oval areas surrounded by the acini. e islets were formed of cords of
cells separated by blood capillaries. e main pancreatic duct was lined
by simple columnar epithelium resting on basement membrane and
surrounded by CT.
e PRP-group (Figure 1c and 1d), showed histological features
that nearly similar to the control group except that some blood vessels
appeared congested and the main pancreatic duct was dilated with wide
lumen and stagnant secretion. Some areas of stratication were noticed
in the lining epithelium.
In the diabetic group (Figure 2), STZ caused variable marked
morphological changes in the pancreatic tissue structure. ere was
widening of the inter-lobular spaces and the islets were less numerous.
Some islets became massively degenerated with a reduction of the cell
mass while others were completely devoid of cells. Dilatation of the
intra-lobular duct and numerous dilated blood vessels loaded with
RBCS and inammatory cells were seen in between the lobules. e
degenerated islets showed ballooned cells with vacuolated cytoplasm
and were separated with congested blood capillaries. Other islets
showed shrunken cells with acidophilic cytoplasm and pyknotic nuclei.
Fibroblast like cells were seen inltrating the islet. e main pancreatic
duct was dilated and had areas of stratication in its epithelial lining.
PRP administration to diabetic rats of the PRP/diabetic group
(Figure 3), resulted in marked improvement in the morphological
changes observed in the diabetic animals. Numerous islets of variable
sizes were seen. Some islets were observed connected to the nearby one
by stream of cells and appeared in close associations with the ducts. e
islet cells appeared normal with scarcely seen vacuolated cells. e duct
system showed several changes. Regarding the main pancreatic duct,
the lining epithelium showed increased stratications, invagination
of the surface epithelium to the underlying connective tissue, and
sprouting of the ductal epithelial cells in between the lobules. Numerous
intra-lobular ducts were dilated and had invaginations in their lining
epithelium. Some acini and islets enclosed within large amount of
connective tissue were noticed in close association with the wall of the
intra-lobular duct. Interestingly, scattered areas of the parenchyma had
begun to be organized into small lobules with abundant connective
tissue. is lobule was formed of aggregates of small acini, small ducts,
and numerous islets.
Sections of the pancreas stained with Masson trichrome from the
control group (Figure 4a and 4b) and PRP-group (Figure 4c and 4d)
revealed delicate collagen bers in the septa between lobules and in-
between acini. ey were also observed surrounding the intra-lobular
ducts, the blood vessels and in the wall of the main pancreatic duct.
ere were traces of delicate collagen bers surrounding the margins of
the islets and in between their cells. e diabetic group (Figure 4e and
4f) showed increased collagen deposition around the mentioned sites.
ere was extensive intra-islet collagen deposition forming strands
of brous tissue dividing the islets into nests of endocrine cells. e
PRP/diabetic group (Figure 5) showed a relative increase in collagen
deposition of the inter-lobular connective tissue, the peri-ductal
collagen bers, the intra-lobular ducts, and the blood vessel. In contrast
there was a decrease in the amount of collagen deposition within the
Citation: El-Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA (2017) TEffect of Platelet Rich Plasma (PRP) Injection on the Endocrine
Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab
8: 730. doi: 10.4172/2155-6156.1000730
Page 4 of 9
Volume 8 • Issue 3 • 1000730
J Diabetes Metab, an open access journal
ISSN: 2155-6156
D
C
P
D
I
P
B
V
P
I
a
b
b
BV
200μ
c
d
s
Figures 1: Photomicrographs of rat pancreatic tissue:
a) Control group showing normal lobular architecture; islets of
Langerhans (IS) and the pancreatic acini (PA). Notice the interlobular
connective tissue (CT), the intra-lobular duct (D) and blood vessel
(BV).
b) The islet’s cells of the control group forming cords (black arrows)
separated by a network of blood capillaries (BC). The inset showing
the main pancreatic duct (pd) lined by simple columnar epithelium
(arrows) and surrounded by connective tissue (C). Notice the
c) basal basophilic (red arrows) and apical acidophilic cytoplasm
(yellow arrows) of pancreatic acinar cells.
d) The PRP-group showing preserved lobular architecture. Notice the
congested blood vessels (BV).
The PRP-group showing numerous blood capillaries (BC) in between the
cords of cells (arrows). Inset showing dilated main pancreatic duct
(pd) with areas of stratication (s) and stagnant secretion (star).
H&E, scale bar: a,c X200 μm; b,d, insets X50 μm.
d
E
a
b
c
e
f
Figure 2: Photomicrographs of rat pancreatic tissue of the diabetic group
showing:
a) Degenerated islets (IS), dilated intra-lobular duct (D) and congested
blood vessel (BV). Notice empty area of massively damaged islet
( E ) a n d w i d e i n t e r - l ob u l a r s p a c e s ( * ).
b) A degenerated islet showing numerous cells with vacuolated
cytoplasm (V) and some cells with densely acidophilic cytoplasm
(arrows).
c) Dilated inter-lobular spaces (*), degenerated islet (IS), inter-lobular
duct (D) and congested dilated blood vessel (B.V).
d) Congested and dilated blood vessel (BV) lled with RBCS (*) and
inammatory cell (arrows). Notice the stagnant secretion in the
inter-lobular duct (D).
e) Another islet showing deeply stained cells with pyknotic nuclei
(blue arrows). Notice the broblast like cells (black arrows)
inltrating between islet's cells.
f) Dilated main pancreatic duct (pd) with areas of stratication (s) in
its epithelial lining.
H&E, scale bar: a,c X200 μm; b,e X20μm; d,f X 0μm.
c
a
b
S
S
d
f
e
Figure 3: Photomicrographs of rat pancreatic tissue of the PRP/diabetic group showing:
Figure 3: Photomicrographs of rat pancreatic tissue of the PRP/diabetic group
showing:
a) Restored lobular architecture with numerous islets (arrows) associated
with intralobular ducts (D). Inset showing higher magnication of a
small islet.
b) Some islets are inter-connected with each other (arrows). Notice the
small duct (D). The inset shows higher magnication of a connecting
area between two islets.
c) An islet with numerous apparently normal cells (IC) and rich
vasculature (BC). Notice the scarcely vacuolated cells (arrow).
d) Increased stratications (S) in the epithelium lining of the main
pancreatic duct (pd), and the invagination of the surface epithelium
to the underlying connective tissue (black arrows). Notice the ductal
epithelial cells in between the lobules (red arrows) .
e) A dilated intra-lobular duct (d) with invagination of its epithelial lining
(arrow). Notice the acini (A) and islets (IS) in a relatively large amount
of connective tissue (CT).
f) An apparently newly formed small lobule containing small ducts (D),
some acini (A) and islets (IS) embedded in connective tissue (arrows).
H&E, scale bar: a,b X200 μm; c,e, inset X50 μm; d,f X100 μm
islet. Some areas had small clusters of endocrine cells closely associated
with the duct and each cluster was encapsulated by connective tissue.
e wall of the main pancreatic duct was invested with a large amount
of collagen bers compared to the control group.
Immunohistochemical study
Immunohistochemical analysis of sections immunostained
for insulin: e insulin secreting cells; β-cells, of the control group
(Figure 6a) and the PRP-group (Figure 6b) represented the major
cell population of the islets. e ductal epithelium showed negative
reaction. In the diabetic group (Figure 6c), some islets showed reduced
immune reactivity while others showed negative expression. e ductal
epithelium had also a negative reaction.
e PRP/diabetic group (Figure 7) showed increased immunostained
cells in the islet compared to the diabetic group. Scattered newly formed
small islets composed of positive cells were observed in the lobules. e
connection of cells that were observed connecting two islets had also
positive expression. ere was a positive expression in the epithelial
lining of the nearby intra-lobular ducts. Some immunostained cells
were observed forming a stream between the islets and the nearby ducts.
Surprisingly, few exocrine acinar cells showed positive expression.
Immunohistochemical analysis of sections immunostained for
PCNA: e control group (Figure 8a and 8b), showed few positive
Citation: El-Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA (2017) TEffect of Platelet Rich Plasma (PRP) Injection on the Endocrine
Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab
8: 730. doi: 10.4172/2155-6156.1000730
Page 5 of 9
Volume 8 • Issue 3 • 1000730
J Diabetes Metab, an open access journal
ISSN: 2155-6156
these positive nuclei had atypical appearance with abnormal shape and
sizes.
In the diabetic group (Figure 8e and 8f), scarce immunopositive cells
were noticed in the islets, while the lining epithelium of intra-lobular duct
and the acinar cells showed numerous immunopositive nuclei.
a
c
e
b
d
f
c
Figure 4: Photomicrographs of rat pancreatic tissue showing collagen
bers in the septa between lobules, between acini (arrows), surrounding the
intralobular ducts (D) and blood vessels (BV) in : a ) Control group, c) The
PRP treated group, e) Diabetic group. Higher magnications showing the
islets and the inset showing the main pancreatic ducts of b) Control group,
d) The PRP -group. The diabetic group: e) Dense collagen deposition around
the congested dilated blood vessel (BV) and the inter-lobular ducts (D). f) The
extensive intra-islet collagen deposition (black arrows) forming strands dividing
the islet into nests of endocrine cells (N). The inset shows the main pancreatic
duct (pd) with extensive collagen (C) bers in its wall. Masson trichrome, scale
bar: a,c X200 μm; b,d,f X50 μm; e, insets X100 μm
a
D
50μ
b
c
d
c
Figure 5: Photomicrographs of rat pancreatic tissue of the PRP/diabetic group
showing:
a) A relative increase in collagen deposition surrounding lobules
(arrows) ,intra-lobular ducts (D) and blood vessel (BV).
b) An islet with little amount of collagen bers (black arrows) in between
islet cells. Notice prominent collagen bers (yellow arrow) around the
duct (D).
c) Small clusters of endocrine cells (C) encapsulated by connective
tissue (arrows ) and closely associated with the intra-lobular duct wall
(D).
d) The main pancreatic duct (pd) with extensive connective tissue rich in
collagen surrounding its wall (C).
Masson trichrome, scale bar: a,d X100 μm; b,c X50 μm
nuclear expression for PCNA in the cells of the islets and in the epithelial
lining of the intra-lobular ducts. In the PRP-group (Figure 8c and 8d),
more immunoreactive nuclei were noticed in the islets of Langerhans
and in the epithelial lining of the intra-lobular ducts. Unfortunately,
200μ
D
200μ
a
c
b
c
200μ
b
D
Figure 6: Photomicrographs of rat pancreatic tissue immunostained for insulin
of:
a) The control group, and b) The PRP-group; showing positive
immunostained β-cells (brown color) occupying most of the islet’s
tissue (black arrows) with negative reaction in the duct (D) and
acini (A). Inset is higher magnication of the positive immunostained
β-cells .
c) The diabetic group showing reduction in the immunohistochemical
expression in one islet (IS) and completely negative expression
in other islets (arrows). Inset showing marked reduction in the
expression in β-cells (arrows). Immunohistochemistry, counterstained
with H, scale bar: a,b,c X200 μm; insets, d X50 μm
a
d
μ
IS
IS
b
c
Figure 7: Photomicrographs of rat pancreatic tissue of the PRP/diabetic group
immunostained for insulin showing:
a) strong reaction in the islet’s cells (IS). Notice numerous small islets
scattered in the lobules (arrows).
b) two islets (IS) communicated to each other by immunopositive cells
(arrow).
c) immunopositive cells (arrows) in the islet (IS) and in the nearby
epithelium lining the intra-lobular duct (D) . Notice stream of
immunopositive cells in between the duct and the islet (arrows).
d) acini (A) and positive expression in two of acinar cells (arrows)
Immunohistochemistry, counterstained with H, scale bar: a,b
X200μm; c X50 μm; d X20 μm.
Citation: El-Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA (2017) TEffect of Platelet Rich Plasma (PRP) Injection on the Endocrine
Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab
8: 730. doi: 10.4172/2155-6156.1000730
Page 6 of 9
Volume 8 • Issue 3 • 1000730
J Diabetes Metab, an open access journal
ISSN: 2155-6156
In the PRP/diabetic group, there was an apparent increase in
positive immunoreactive nuclei among the cells of the islets and in
the intra-lobular ductal lining epithelium. Some immunostained cells
appeared as a stream of positive nuclei that were crawling from the duct
towards the islet. Interestingly the connective tissue surrounding the
inter-lobular duct and cells of acini showed immunopositive reaction
(Figure 8g).
Morphometrical results
e number of islets per square millimeter (islets/mm2): ere
was no signicant dierence in the mean number of pancreatic islets/
mm2 of the PRP-group compared to the control group (p=1.068) but
there was a signicant decrease in the diabetic group compared to the
control group (p=0.001). On the other hand, there was a signicant
increase in the mean number of pancreatic islets in PRP/diabetic group
compared to both the control group (p=0.001) and diabetic1group
(p=0.0001) (Tables 1 and 2).
e number of insulin positive β-cells per islet: ere was no
signicant dierence in the mean number of anti-insulin positive
β-cells of the PRP-group compared to the control group (p=0.200),
but a signicant decrease in the mean number of immunopositive
β-cells in the diabetic group compared to the control group (p=0.0001)
200μ
b
a
50μ
IS
IS
c
50μ
d
50μ
*
*
50μ
e
f
g
Figure 8: Photomicrographs of rat pancreatic tissue immunostained for
PCNA of: a) The control group showing few immunoreactive nuclei in the
islet’s cells (arrows), and b) few immunoreactive nuclei in the epithelial lining
of the inter-lobular duct (arrow) and negative expression in the acini (A).
c) The PRP-group showing numerous islet cells (IS) with positive nuclear
expression. Notice atypical abnormal shape and size of nuclei (arrows), and
d) some immunopositive nuclei in the epithelium lining of the intralobular
duct. e) The diabetic group showing immunoreactive nuclei of islet cells
(arrows) and in the acinar cells (*), and f) immunoreactive nuclei in the lining
epithelium of intra-lobular duct (arrows). g) The PRP/diabetic group showing
many immunoreactive nuclei in the lining epithelium of the intralobular duct
(D) crawling towards the islet (red arrows) and among the islet’s cells (black
arrows). Immunohistochemistry, counterstained with H, scale bar: a,c,d,e,g
X50 μm; b,f X100 μm.
Blood glucose levels (mg/dl)
Mean ± sd p-value
Control group 93.4 ± 12.6
PRP-group 91.3 ± 12.5 0.900c
Diabetic group 393.3 ± 14.9 0.0001c*
PRP/diabetic group 167 ± 54.4 0.046 c*
0.0001d*
*p<0.05 is signicant, c versus the control group, and d versus the diabetic group.
Table 1: The blood glucose levels (mg/dl) in the studied groups (n=10).
was observed. On the other hand, there was a signicant increase in
the mean number of immunoreactive β-cells of PRP/diabetic group
compared to both the control group (p=0.046) and the diabetic group
(p=0.0001).
e number of PCNA positive cells per islet: ere was a
signicant increase in the mean number of PCNA positive cells in the
islets of PRP-group compared to control group (p=0.040). ere was
a signicant decrease in the diabetic group compared to the control
group (p=0.001). On the other hand there was a signicant increase
in the mean number of PCNA positive cells in the islets of PRP/
diabetic group compared to both the control and the diabetic group
(all p=0.000).
Discussion
Platelet rich plasma (PRP) is an autogenous and economical source
of growth factors which nowadays used in the tissue repair [12]. In this
study, the PRP preparation depended on double centrifugation method
that resulted in a platelet concentration 3 times higher than the initial
blood sample [17]. It was also depended on the endogenous activation
of platelets to allow the platelets to make their action spontaneously.
e repeated dose regimen provided a constant elevated level rather
than a sudden ood of growth factors [22].
In pancreatic disorders, there were pancreatic microcirculatory
disturbances and oxidative stress production which resulted in
congestion, extravasation of RBCs, and escape of tissue uids. ese
changes led to widening of the interlobular spaces with inammatory
cell inltration [23]. is was observed in the diabetic group beside the
extensive islet destruction. e STZ induced hyperglycemia by β-cells
destruction [24].
In terms of β-cell regeneration, the present results demonstrated
the eect of PRP injection on the regeneration and restoration of
pancreatic islet cell mass and claried the dierent mechanisms
through which PRP inuence the improvement of the diabetic damage
eects.
New islet cells could develop from progenitors through a process
called neo-genesis in postnatal life in rodent studies (Bonner-Weir et
al. [25]). e GFs were used to stimulate pancreatic β-cell proliferation
in vivo. ese molecules such as vascular endothelial growth factor
(VEGF) and connective tissue growth factor, had been investigated
as potential therapies for diabetes. It can stimulate β-cell proliferation
and insulin production [7]. Administration of PRP to the diabetic
rats in our study showed improvement of the pancreas and the islet
morphology. Numerous islets approached the corresponding healthy
pancreatic sections with an increase in β-cell number. Sections showed
increased positive PCNA cells; increased proliferation. Numerous
cells were functioning cells as the anti-insulin positive cells; β-cells,
represented the major cell population of the islets. Hyperplastic and
proliferative changes occurred in pancreas under the eect of certain
stressors or stimuli led to formation of recent β-cells (Jurczyk et al.
Citation: El-Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA (2017) TEffect of Platelet Rich Plasma (PRP) Injection on the Endocrine
Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab
8: 730. doi: 10.4172/2155-6156.1000730
Page 7 of 9
Volume 8 • Issue 3 • 1000730
J Diabetes Metab, an open access journal
ISSN: 2155-6156
[26]). erefore, this study suggested that GFs of the PRP might act as
stimuli for proliferation of these cells that led to an increase in β-cells
number.
e pancreatic duct cells may serve as a source of regeneration
in adult rats by undergoing a reproducible trans-dierentiation into
β-cells [27]. β-cell progenitors located in the ductal lining epithelium
could be activated in the injured adult mouse pancreas (Xu et al. [28]).
In this study, the ductal epithelial lining showed areas of stratications
of the main pancreatic duct in the diabetic group which might be a
trial to increase the cell numbers as a way for regeneration of β-cells.
However, the capacity of the pancreas to regenerate was limited and the
exhaustion of cells led to diabetes because of the imbalance between ß
cell destruction and ß cell formation [29].
In the PRP/diabetic group, several areas of stratication and
invagination of the epithelial lining of the ducts were observed and
most of the islets and small clusters of endocrine cells were seen
closely associated with ducts. PCNA immunostained sections showed
increased positive cells in the lining epithelium of the intra-lobular
duct. Interestingly, a stream of PCNA-positive cells appeared as if
it was crawling from the duct towards the nearby islet through the
connective tissue between them. Furthermore, numerous cells in the
connective tissue showed insulin immunereactivity. ese ndings
could be claried by lineage-tracing data described by Xu et al. [28] who
mentioned that in adult pancreas some cells originate from hormone
progenitors present near ducts and become hormone-positive islet cells
in adult mice. is enforced our suggestion that platelet GFs released in
injured pancreas might act as stimuli for ductal stem cell activation and
its further dierentiation. is was in agreement with Song et al. [30]
who reported that the TGF-α; one of PRP GFs, induced the expansion
of ductal cells leading to an increase of areas of islet in a process of
neogenesis.
In addition, some acini in our results had PCNA positive cells
and some insulin positive cells in their lining. Acinar cells could be
converted to mature β-cells aer injury as a compensatory mechanism
[31]. Acinar to endocrine conversion was demonstrated using in
vitro cultured primary acinar cells treated with GFs such as EGF.
Accordingly, endocrine cells can regularly be detected in exocrine cells
in each rodents and humans [32].
e ndings of this study were in line with Jurczyk et al. [26]
who found that the histological studies on human tissue in adult who
exposed to any stress factors showed insulin-expressing cells in ducts,
isolated β-cells in the pancreatic parenchyma, and small islet clusters.
PRP had consistently shown to potentiate stem cell proliferation,
migration, and dierentiation (Masoudi et al. [33]). e PRP peptide
GFs such as EGF were the principal external indicators that activate the
mitogen-activated protein kinase and had a role in trans-dierentiation
of pancreatic acinar and ductal cells into endocrine islet cells (Abban-
Mete et al. [34]). Another one of PRP GFs; insulin growth factor,
localized to the focal areas of regeneration and may play an important
role in pancreatic regeneration by autocrine mechanisms through
stimulation of DNA synthesis [35]. is could take place by using
replication of already dierentiated β-cells (β-cell plasticity) or neo-
genesis from putative islet stem cells in the ductal or acinar epithelium
[26].
erefore, PRP could restore β-cells by stimulating the dierent
sources of β-cells progenerators in adult rat through proliferation and
trans-dierentiation process. is was in contrast to others [36,37] who
suggested that adult pancreatic β-cells are formed by self-duplication
rather than stem-cell dierentiation. Also, it was in opposing to Kopp
et al. [37] who reported that the derivation of endocrine cells from the
ducts occurred only in early postnatal life, but no endocrine or acinar
cell neo-genesis occurred in adult mice either physiologically or aer
pancreatic duct ligation.
For assessment of the role of the PRP injection in stimulation of
extracellular matrix formation in pancreatic tissues, Masson trichrome
stain was used. Aer STZ injection in the diabetic group, extensive
collagen depositions were observed. In diabetes, there was inter-acinar
and inter-lobular brosis which suggesting that insulinopenia might
cause these injurious eects on exocrine pancreas [38]. Concerning
this issue, previous reports localized the pancreatic stellate cell (PSCs)
in the islets. Upon activation, PSCs started to proliferate, change their
morphology into myobroblast-like cells, and start to secrete extra
cellular matrix components [39].
In this study, broblast-like cells were seen invading some islets.
is observation could explain the extensive intra-islet collagen
deposition forming strands of brous tissue which divided the islets
into nests of endocrine cells. Kim et al. [40] in their study owed the
brotic changes to activation and proliferation of the PSCs. Invasions
of the pancreatic islets by these cells resulted in brotic islet destruction
that led to the limited capacity of β-cell proliferation and accelerated
apoptosis in diabetic patients. is was responsible of delaying a
complete tissue restoration in diabetes [41].
Activated PSCs subsequently develop functional alterations
including: increased proliferation and migration, synthesis of excessive
ECM proteins (collagen, bronectin, laminin), secretion of GFs and
cytokines which exert both paracrine and autocrine eects to enhance
the cell growth and migration [42]. In our study, PRP/diabetic group
revealed an obvious decrease in collagen deposition in the islet and a
relative increase in the peri-ductal collagen and within the inter-lobular
connective tissue.
e increased vasculature observed in this group could be explained
by Bir et al. [43] results who mentioned that injection of PRP resulted
in increasing the neo-vascularization due to release of VEGF.
One of the most characteristic ndings of this work was the
appearance of aggregates of small acini, small ducts, and numerous small
islets/m2β-cells/islet PCNA +ve cells/islet
Mean ± SD p-value Mean ± SD p-value Mean ± SD p-value
Control group 2.5 ± 0.97 88.7 ± 8.7 5.9 ± 3.1
PRP-group 2.5 ± 0.85 1.068c 94 ± 7.5 0.200c 8.5 ± 6.1 0.040c*
Diabetic group 1 ± 0.85 0.001c* 23.6 ± 22.8 0.0001c* 1.2 ± 1.8 0.001c*
PRP/diabetic group 4.4 ± 0.96 0.001c*
0.0001d* 104.50 ± 21.9 0.046 c*
0.0001d* 24.1 ± 6.6 0.0001c*
0.0001d*
*p<0.05 is signicant. cversus the control group. d versus the diabetic group.
Table 2: The number of iselts per square millimeter (islets/m2), insulin positive β-cells per islet (β-cells/islet), and PCNA
positive cells per islet (PCNA +ve cells/islet) in the studied groups (n=10).
Citation: El-Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA (2017) TEffect of Platelet Rich Plasma (PRP) Injection on the Endocrine
Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab
8: 730. doi: 10.4172/2155-6156.1000730
Page 8 of 9
Volume 8 • Issue 3 • 1000730
J Diabetes Metab, an open access journal
ISSN: 2155-6156
islets abundant connective tissue arranged into small lobules. ese
results could provide an evidence of new lobule formation through
PRP activation of ECM formation together with the neovascularization
in order to build a scaold for the proliferated progenitors in the
ducts. en the ductal epithelium gives rise to all pancreatic epithelial
lineages, i.e. duct, acinar and endocrine cells [44]. ese were similar to
Hardikar, who described the same consequence for endocrine pancreas
development during the postnatal period. erefore, this could support
our suggestion that the PRP administration might put the pancreas in
an environment similar to the postnatal period of development.
Regarding the morphometric results of this study, two important
observations must be taken in consideration: in the PRP/diabetic
group there was signicant increase in the number of PCNA positive
cells compared to controls. erefore, its recommended to perform
several studies for adequate adjustment of the dose and duration of
PRP therapy before its clinical application. e PRP-group exhibited
increased PCNA positive cells which had atypical shape and size of
nuclei. erefore, our study recommends not to inject the PRP in
normal conditions to avoid overstimulation of cell proliferation, which
could lead to hyperplasia or tumor formation.
Conclusion
is study provides an evidence of the diabetic pancreatic islet
regeneration in response to PRP treatment. e PRP stimulated islet cell
regeneration and stimulated the induction of other sources of β-cells
generation as the exocrine portion of the pancreas; ductal and acinar
cells. In addition, PRP might put the pancreas into an environment
similar to the postnatal developmental one where new lobules were
formed. ese will pave the future for a novel treatment for diabetes.
Recommendations
Some limitations to this study were acknowledged. Further
studies are required to identify the exact mechanisms responsible for
these results and to conrm the process of neo-genesis and trans-
dierentiation among pancreatic cells.
Conicts of Interest
ere is no conict of interest to declare.
References
1. WHO (2016) Global report on diabetes. Retrieved January 1, 2017, from World
Health Organization 21: 10-61.
2. Patel D, Kumar R, Laloo D, Hemalatha S (2012) Diabetes mellitus: An
overview on its pharmacological aspects and reported medicinal plants having
antidiabetic activity. Asian Pacic Journal of Tropical Biomedicine 2: 411-420.
3. Bender C, Christen S, Scholich K, Bayer M, Pfeilschifter JM, et al. (2016) Islet-
Expressed CXCL10 Promotes Autoimmune Destruction of Islet Isografts in
Mice With Type 1 Diabetes. Diabetes 66: 113-126.
4. Pagliuca F, Millman J, Gürtler M, Segel M, Van Dervort A, et al. (2014)
Generation of Functional Human Pancreatic β Cells In Vitro Cell. Pancreatic
disorders and therapy 159: 428-439.
5. Okere B, Lucaccioni L, Dominici M, Iughetti L (2016) Cell therapies for
pancreatic beta-cell replenishment Italian. Journal of Pediatrics 42: 18-21.
6. Pagliuca FW, Melton DA (2013) How to make a functional β-cell Development.
Journal of endocrinology 140: 2472-2483.
7. Márquez-Aguirre A, Canales-Aguirre A, Padilla-Camberos E, Esquivel-Solis H,
Díaz-Martínez N (2015) Development of the endocrine pancreas and novel
strategies for β-cell mass restoration and diabetes therapy. Brazilian Journal of
Medical and Biological Research 48:765-776.
8. Goud, BJ, Dwarakanath M, Chikk swamy K (2015) Streptozotocin - A
Diabetogenic Agent in Animal Models. Human Journals Review article 3: 253-
269.
9. Mani R, Mahantesha S, Nandini TK, Lavanya R (2014) Growth Factors in
Periodontal Regeneration. Journal of Advanced Oral Research 5: 1-5.
10. Zhang M, Lin Q, Qi T, Wang, T, Chen C, et al. (2016) Growth factors and
medium hyperglycemia induce Sox9+ductal cell differentiation into β cells
in mice with reversal of diabetes. Proceedings of the National Academy of
Sciences 113: 650-655.
11. Sonker A, Dubey A, Bhatnagar A, Chaudhary R (2015) Platelet growth factors
from allogeneic platelet-rich plasma for clinical improvement in split-thickness
skin graft. Asian Journal of Transfusion Science 9:155-158.
12. Cavallo C, Rof A, Grigolo B, Mariani E, Pratelli L, et al. (2016) Platelet-Rich
Plasma: The Choice of Activation Method Affects the Release of Bioactive
Molecules. BioMed Research International 1-7.
13. Knezevic NN, Candido KD, Desai R, Kaye AD (2016) Is Platelet-Rich Plasma
a Future Therapy in Pain Management. Medical Clinics of North America 100:
199-217.
14. Pavlovic V, Ciric M, Jovanovic V, Stojanovic P (2016) Platelet rich plasma: A
short overview of certain bioactive components. Open Medicine 11: 242-247.
15. Redaelli A, Romano D, Marciano A (2010) Face and neck revitalization with
platelet-rich plasma (PRP): clinical outcome in a series of 23 consecutively
treated patients. J Drugs Dermatol 9: 466-472.
16. Ukwenya V, Ashaolu O, Adeyemi D, Abraham K (2015) Experimental diabetes
and the epididymis of Wistar rats: The protective effects of Anacardium
occidentale (Linn). Journal of Experimental and Clinical Anatomy 14: 57.
17. Pazzini JM, Nardi AB, Huppes RR, Gering AP, Ferreira MG, et al. (2016) Method
to obtain platelet-rich plasma from rabbits (Oryctolagus cuniculus). Pesquisa
Veterinária Brasileira 36: 39-44.
18. Hesami Z, Jamshidzadeh A, Ayatollahi M, Geramizadeh B, Farshad O, et al.
(2014) Effect of Platelet-Rich Plasma on CCl 4 -Induced Chronic Liver Injury in
Male Rats. International Journal of Hepatology 14: 1-7.
19. Attia H, Al-Rasheed N, Al-Rasheed N, Faddah L (2015) The combination of
zinc and glibenclamide limits cardiovascular complications in diabetic rats via
multiple mechanisms. Pak J Pharm Sci 28: 499-508.
20. Bancroft JD, Suvarna S, Kim Layton C (2013) Bancroft’s Theory and Practice of
Histological Techniques China: Churchill Livingstone. Elsevier 5: 6-10.
21. Cemek M, Kağa S, Şimşek N, Buyukokuroğlu ME, Konuk M (2008)
Antihyperglycemic and antioxidative potential of Matricaria chamomilla L in
streptozotocin-induced diabetic rats. Journal of Natural Medicines 62: 284-293.
22. Hammond JW, Hinton RY, Curl LA, Muriel JM, Lovering RM (2009) Use of
Autologous Platelet-rich Plasma to Treat Muscle Strain Injuries. The American
Journal of Sports Medicine 37: 1135-1142.
23. Zhou ZG, Chen YD, Sun W, Chen Z (2002) Pancreatic microcirculatory
impairment in experimental acute pancreatitis in rats World. Journal of
Gastroenterology 8: 933-936.
24. Rifaai RA, El-Tahawy NF, Saber EA, Ahmed R (2012): Effect of Quercetin on
the Endocrine Pancreas of the Experimentally Induced Diabetesin Male Albino
Rats: A Histological and Immunohistochemical Study. J Diabetes Metab 3: 182-
193.
25. Bonner-Weir S, Li W, Ouziel-Yahalom L, Guo L, Weir GC, et al. (2010) Cell
Growth and Regeneration: Replication Is Only Part of the Story Diabetes 59:
2340-2348.
26. Jurczyk A, Bortell R, Alonso LC (2014) Human β-cell regeneration: progress,
hurdles, and controversy Current Opinion in Endocrinology & Diabetes and
Obesity. J Diab Metab 21: 102-108.
27. Juhl K, Bonner-Weir S, Sharma A (2010) Regenerating pancreatic
β-cells: plasticity of adult pancreatic cells and the feasibility of in-vivo
neogenesis. Current Opinion in Organ Transplantation 15: 79-85.
28. Xu X, D'Hoker J, Stangé G, Bonné S, De Leu N, et al. (2008) β Cells Can
Be Generated from Endogenous Progenitors in Injured Adult Mouse Pancreas
Cell. American Journal of Diabetes 132: 197-207.
29. Risbud MV, Bhonde RR (2002) Models of pancreatic regeneration in
diabetes. Diabetes Research and Clinical Practice 58: 155-165.
Citation: El-Tahawy NF, Rifaai RA, Saber EA, Saied SR, Ibrahim RA (2017) TEffect of Platelet Rich Plasma (PRP) Injection on the Endocrine
Pancreas of the Experimentally Induced Diabetes in Male Albino Rats: A Histological and Immunohistochemical Study. J Diabetes Metab
8: 730. doi: 10.4172/2155-6156.1000730
Page 9 of 9
Volume 8 • Issue 3 • 1000730
J Diabetes Metab, an open access journal
ISSN: 2155-6156
30. Song SY, Gannon M, Washington M, Scoggins CR, Meszoely IM et al.
(1999) Expansion of Pdx1-expressing pancreatic epithelium and islet
neogenesis in transgenic mice overexpressing transforming growth factor. Α
Gastroenterology 117: 1416-1426.
31. Pan FC, Bankaitis ED, Boyer D, Xu X, Van de Casteele M, et al. (2013)
Spatiotemporal patterns of multipotentiality in Ptf1a-expressing cells
during pancreas organogenesis and injury-induced facultative restoration
Development 140: 751-764.
32. Meier JJ, Bhushan A, Butler AE, Rizza RA, Butler PC (2005) Sustained beta
cell apoptosis in patients with long-standing type 1 diabetes: indirect evidence
for islet regeneration. Diabetologia 48: 2221-2228.
33. Masoudi EA, Ribas J, Kaushik G, Leijten J, Khademhosseini A (2016)
Platelet-Rich Blood Derivatives for Stem Cell-Based Tissue Engineering and
Regeneration. Current Stem Cell Reports 2: 33-42.
34. Abban-Mete G, Erdogan D, Cam M, Ozogul C (2013) The Effects of Epidermal
Growth Factor on Pancreas in Alloxan- Diabetic Rats: An Ultrastructural
Study. Journal of Endocrinology and Diabetes Mellitus 1: 27-32.
35. George M, Ayuso E, Casellas A, Costa C, Devedjian JC, et al. (2002) β cell
expression of IGF-I leads to recovery from type 1 diabetes. Journal of Clinical
Investigation 109: 1153-1163.
36. Dor Y, Brown J, Martinez OI, Melton DA (2004) Adult pancreatic β-cells are formed
by self-duplication rather than stem-cell differentiation Nature 429: 41-46.
37. Kopp JL, Dubois CL, Schaffer AE, Hao E, Shih HP, et al. (2011) Sox9+
ductal cells are multipotent progenitors throughout development but do
not produce new endocrine cells in the normal or injured adult pancreas
Development. Journal of Gastroenterology 138: 653-665.
38. Riccillo FL, Bracamonte MI, Console GM, Gómez Dumm CL (2004)
Histomorphological and quantitative immunohistochemical changes in the rat
pancreas during aging Biocell. Journal of Endocrinology and Diabetes 28: 127-
134.
39. Apte M, Pirola R, Wilson J (2009) New insights into alcoholic pancreatitis and
pancreatic cancer Journal of Gastroenterology and Hepatology 24: 51-56.
40. Kim A, Miller K, Jo J, Kilimnik G, Wojcik P, Hara M (2009) Islet architecture: A
comparative study Islets. Journal of Gastroenterology 1: 129-136.
Citation: Ludwig C, Streicher M, Habicht SD, Swai ME, Krawinkel MB (2017)
Targeted screening reveals high numbers of prediabetes and diabetes mellitus
in Moshi, Tanzania. J Diabetes Metab 8: 720. doi: 10.4172/2155-6156.1000720
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