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Supplementary file 2.

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Abstract

Oligonucleotides used in this study for cloning purposes. DOI: http://dx.doi.org/10.7554/eLife.20083.023
Supplementary Table 2. Oligonucleotides used in this study for cloning purposes
Oligonucleotides Sequence
ap2σΔ::AFpyrG / ap2σΔ::AFriboB
ap2σ 5’ SphI F CGCGGCATGCGAAGAATCGCGTTAATGTCTCGGG
ap2σ 5’ SpeI R CGCGACTAGTCTTGTCTGATGGCCCGCGGTGCTC
ap2σ 3’ SpeI F CGCGACTAGTTTATTGTTCATGTGATAAGTGAACGG
ap2σ 3’ NdeI R CGCGCATATGGAGATACCAGGAGTAGCCCC
AFriboB SpeI F CGCGACTAGTAAGCTTGATATCACAATCAGC
AFriboB SpeI R CGCGACTAGTCCCGGGCTGCAGGAATTCGATAAG
AFpyrG SpeI F CGCGACTAGTGCCTCAAACAATGCTCTTCACCCTC
AFpyrG SpeI R CGCGACTAGTACTGTCTGAGAGGAGGCACTGATGCG
ap2σ-(5xGA)GFP::AFpyrG / ap2σ-(5xGA)mRFP::AFpyrG
ap2σ 5’ KpnI F CGCGGGTACCGTCGTAATATAACTCGTACAGCAATC
ap2σ 3’ ORF SpeI NS R CGCGACTAGTCTCCAGTTTATCCAGATGTTCTAGCC
ap2σ 3’ SpeI F CGCGACTAGTTTATTGTTCATGTGATAAGTGAACGGCTGC
ap2σ 3‘NotI R CGCGGCGGCCGCGAGATACCAGGAGTAGCCCCGCTG
5xGA SpeI F CGCGACTAGTGGAGCTGGTGCAGGCGCTGGAGCCGGTGCC
AFpyrG SpeI R CGCGACTAGTACTGTCTGAGAGGAGGCACTGATGCG
ap2μΔ::AFpyrG / ap2μΔ::AFriboB
ap2μ 5’ KpnI F CGCGGGTACCCCTTACCCTCATCCGACTCCGAAC
ap2μ 5’ SpeI R CGCGACTAGTTTTGCGATTAGGCGTGGTGGTAAGG
ap2μ 3’ SpeI F CGCGACTAGTGGCTGATGCTAACATATATATTTGCAG
ap2μ 3’ NotI R CGCGGCGGCCGCCCACTCCCACTGATGACAGTCAATG
AFriboB SpeI F CGCGACTAGTAAGCTTGATATCACAATCAGC
AFriboB SpeI R CGCGACTAGTCCCGGGCTGCAGGAATTCGATAAG
AFpyrG SpeI F CGCGACTAGTGCCTCAAACAATGCTCTTCACCCTC
AFpyrG SpeI R CGCGACTAGTACTGTCTGAGAGGAGGCACTGATGCG
Ap3σΔ::AFpyrG
ap3σ 5’ ApaI F CGCGGGGCCCGAGTCCGCACTCAGCAGCTC
ap3σ 5’ SpeI R CGCGACTAGTTTCGGATATAGGCTGATGGTG
ap3σ 3’ SpeI F CGCGACTAGTGGTTGTACAGCTTCTCCGTG
ap3σ 3’ NdeI R CGCGCATATGGGGAAGGAAAACAACCAGCTC
thiAp-ap1σ::AFriboB
ap1σ 5’ ApaI F CGCGGGGCCCGGCAGGACTGCTGTTAGGGAGC
ap1σ 5’ SphI R CGCGGCATGCTTTGCAGATTGTGGGAAAAGTCGTGG
ap1σ ORF SacII F CGGCCCGCGGATGGCAATTCAGTAAGACTTGAATGC
ap1σ 3’ NotI R CGCGGCGGCCGCGGGTAAGTGATCCCTGTTGTCTTAGC
AFriboB SphI F CCCGGGCATGCAAGCTTGATATCACAATCAGC
AFriboB SphI R CCCGGGCATGCCCCGGGCTGCAGGAATTCGATAAG
thiAp SphI F CGGGCATGCCGACCTGGCACCTACAGAAGAATCC
thiAp FLAG SacII R CGCGCCGCGGCTTGTCATCGTCGTCCTTGTAGTCCAT
GTTGACTCAGTTCAATGGTTCGACTATAG
claLΔ::AFpyrG
claL 5’ NcoI F CGCGCCATGGCCCTCGCATAATCGCATCCTCTAC
claL 5’ SpeI R CGCGACTAGTCCTCCTTGTAATATAGGATATACGACG
claL 3’ SpeI F CGCGACTAGTCTGCTGATTCCCTAATATTCTGGCC
claL 3’ NotI R CGCGGCGGCCGCCCAGGTCAAAGCCGAGGTTGAAG
AFpyrG SpeI F CGCGACTAGTGCCTCAAACAATGCTCTTCACCCTC
AFpyrG SpeI R CGCGACTAGTACTGTCTGAGAGGAGGCACTGATGCG
thiAp-claL::AFpyrG / thiAp-claL::AFriboB
claL 5’ NcoI F CGCGCCATGGCCCTCGCATAATCGCATCCTCTAC
claL 5’ SpeI R CGCGACTAGTCCTCCTTGTAATATAGGATATACGACG
claL ORF SpeI F CGCGACTAGTATGGCTGACCGCTTCCCGTCGTTG
claL 3’ NotI R CGCGGCGGCCGCCCAGGTCAAAGCCGAGGTTGAAG
thiAp XbaI F CGCGTCTAGACGACCTGGCACCTACAGAAGAATCC
thiAp SpeI R CGCGACTAGTGTTGACTCAGTTCAATGGTTCGAC
AFpyrG SpeI F CGCGACTAGTGCCTCAAACAATGCTCTTCACCCTC
AFpyrG XbaI R CGCGTCTAGAACTGTCTGAGAGGAGGCACTGATGCG
AFriboB SpeI F CGCGACTAGTAAGCTTGATATCACAATCAGC
AFriboB XbaI R CGCGTCTAGACCCGGGCTGCAGGAATTCGATAAG
claL-(5xGA)GFP::AFpyrG
ClaL 5’ NcoI F CGCGCCATGGCCCTCGCATAATCGCATCCTCTAC
ClaL ORF NS SpeI R CGCGACTAGTAACCCCGCTAGCGCCAGGCGCTC
ClaL 3’ SpeI F CGCGACTAGTCTGCTGATTCCCTAATATTCTGGCC
ClaL 3’ NotI R CGCGGCGGCCGCCCAGGTCAAAGCCGAGGTTGAAG
5xGA SpeI F CGCGACTAGTGGAGCTGGTGCAGGCGCTGGAGCCGGTGCC
AFpyrG SpeI R CGCGACTAGTACTGTCTGAGAGGAGGCACTGATGCG
claHΔ::AFpyrG
ClaH 5 ApaI F CGCGGGGCCCGGCAAGTACCTTGTCCTTCAAATGG
ClaH 5 SpeI R GATGACTAGTGTTGCAGCTGTGAAGTTG
ClaH ORF2 SpeI F CGCGACTAGTGAGGAACTGGGTGATATTGTCCGACC
ClaH ORF2 NotI R CGCGGCGGCCGCGCAGTGGCAACAACCTGGTCAATGAGAG
AFpyrG SpeI F CGCGACTAGTGCCTCAAACAATGCTCTTCACCCTC
AFpyrG SpeI R CGCGACTAGTACTGTCTGAGAGGAGGCACTGATGCG
thiAp-claH::AFpyroA
ClaH 5 ApaI F CGCGGGGCCCGGCAAGTACCTTGTCCTTCAAATGG
ClaH 5 SpeI R GATGACTAGTGTTGCAGCTGTGAAGTTG
ClaH ORF SpeI F CGCGACTAGTATGGCTCCTCTTCCCATCAAATTCAC
ClaH ORF NotI R CGCGGCGGCCGCCTTGTTGATTGTCTCTGGCGTCCTC
AFpyroA XbaI F CGCGTCTAGAGGACATCAGATGCTGGATTAC
AFpyroA SpeI R CGCGACTAGTGCGAGTGTCTACATAATGAAGG
thiAp XbaI F CGCGTCTAGACGACCTGGCACCTACAGAAGAATCC
thiAp SpeI R CGCGACTAGTGTTGACTCAGTTCAATGGTTCGAC
thiAp-basA::AFpyrG / thiAp-basA::AFriboB
basA 5’ ApaI F GCGCGGGCCCGAGCGTACTCTTCAGGTGACCCTTG
basA 5’ SpeI R2 CGCGACTAGTGCAACGTCAATTAGGACGTCGG
basA ORF SpeI F CGCGACTAGTATGGCTACAAACACAACTTTGCTCTATGATC
basA 3’ NotI R CGCGGCGGCCGCCGTGGATCGGTTAGGCATGCATATG
thiAp XbaI F CGCGTCTAGACGACCTGGCACCTACAGAAGAATCC
thiAp SpeI R CGCGACTAGTGTTGACTCAGTTCAATGGTTCGAC
AFpyrG SpeI F CGCGACTAGTGCCTCAAACAATGCTCTTCACCCTC
AFpyrG XbaI R CGCGTCTAGAACTGTCTGAGAGGAGGCACTGATGCG
AFriboB SpeI F CGCGACTAGTAAGCTTGATATCACAATCAGC
AFriboB XbaI R CGCGTCTAGACCCGGGCTGCAGGAATTCGATAAG
thiAp-slaB::AFpyrG / thiAp-slaB::AFriboB
slaB 5 KpnI F2 CGCGGGTACCCGATGATTGAGATATCCCGCCGGTC
slaB 5 SpeI R3 CGCGACTAGTCAGACCTCCTAAAGTCCGCGGGTCTTG
slaB ORF SpeI F2 CGCGACTAGTATGAGTCGGTAGGTAATTGGGGACTG
slaB ORF SacI R CGCGGAGCTCCATACTTGCTTCTCCATGTGTTGAC
thiAp XbaI F CGCGTCTAGACGACCTGGCACCTACAGAAGAATCC
thiAp SpeI R CGCGACTAGTGTTGACTCAGTTCAATGGTTCGAC
AFpyrG SpeI F CGCGACTAGTGCCTCAAACAATGCTCTTCACCCTC
AFpyrG XbaI R CGCGTCTAGAACTGTCTGAGAGGAGGCACTGATGCG
AFriboB SpeI F CGCGACTAGTAAGCTTGATATCACAATCAGC
AFriboB XbaI R CGCGTCTAGACCCGGGCTGCAGGAATTCGATAAG
ap2σ-(5xGA)GFP::AFpyrG, claL-(5xGA)GFP::AFpyrG and ap2σ-
(5xGA)mRFP::AFpyrG constructs carry a 5x Gly-Ala (5xGA) linker, amplified
together with GFP or mRFP and AFpyrG from plasmids p1439, or p1491 respectively
(Szewczyk et al., 2006).

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