Introduction: Over the past 60 years, several studies show a reduction in the concentration of sperm in many countries industrializzati.In some countries , especially industrialized , this trend shows differences of areas within the same country or even in the same region .
The EU project " EcoFoodFertility " affect 8 areas in Italy, Spain , Greece, Rep.Ceca.L ' male reproductive system is very sensitive to changes in endogenous and exogenous , and in particular , in the seminal fluid , it is possible to verify and quantify the presence of contaminants environmental and simultaneously measure the direct effects on sperm noxae very sensitive to the environment, therefore elective bioindicators of environmental damage on human health.
Materials and Methods: In this study, multi-disciplinary and multi- bodied males from 20 to 40 years old, non-smoking, non-drinking , non- drug , non-occupationally exposed , from 4 areas of high environmental impact, will be recruited from Italy ( Land of fires Campania ), Spain (area ind.Terrassa ), Greece ( Athens) and Czech Republic ( Silesia ) and 4 corresponding areas of low impact .
It will measure the levels of contaminants in the seed and in the blood ( heavy metals , polycyclic aromatic hydrocarbons Dioxins , PCBs and nanoparticles ) and it will correlate with conventional semen parameters , redox state , and especially with the index of DNA fragmentation spermatozoario . Similarly for blood lymphocytes will be made karyotyping and chromosomal stability by cytogenetic tests . We will also measure the antitransglutaminase and cytokines for food intolerances , especially gluten sensitive and celiac disease research .
Conclusions and objectives : The study, in addition to being able to give a knowledge of the state of bioaccumulation of environmental contaminants in homogeneous populations in areas with different environmental pressure index in both the countries themselves and between different countries, intends to investigate more clearly , how and how much environmental pollution directly affects the quality of semen , whether, how and what affects the development of food intolerances , such as eating styles and impact on reproductive function and, again, what and most reliable biomarker of cellular stress are associated with the bioaccumulation of contaminants environmental in blood and seme.In Finally, the role of diet and the possibilities offered by innovative formulations of nutritional sciences .