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SAARC J. Agri., 11(1): 1-6 (2013)
GROWTH, YIELD AND NUTRIENT CONTENT OF
BLACKGRAM (Vigna mungo) AS INFLUENCED BY
LEVELS OF PHOSPHORUS, SULPHUR AND
PHOSPHORUS SOLUBILIZING BACTERIA
A. H. Mir1, S. B. Lal, M. Salmani, M. Abid and I. Khan
Department of Soil & Environmental Sciences, Allahabad Agricultural Institute-Deemed University,
Allahabad, 211007, India
ABSTRACT
A field experiment was conducted at Allahabad Agricultural Institute-
Deemed University, Allahabad to study the effect of levels of
phosphorus, sulphur and Phosphorus Solubilizing Bacteria (PSB) on
growth, yield and nutrient content of blackgram for consecutive two years
2004 and 2005. The crop growth parameters viz., plant height, number of
nodules and number of leaves per plant, yield and nutrient content
increased significantly with the application of high levels of phosphorus,
sulphur with or without bio-fertilizer inoculation. Application of 60 kg P2O5
ha-1 recorded maximum plant height (49.9 cm), number of leaves plant-1
(50.8), number of nodules plant-1(27.8), haulm yield (28.9 q ha-1), grain
yield (8 q ha-1) and phosphorus, sulphur and protein content of grain
(0.356 %, 0.253% and 22.64%, respectively) as compared to lower
levels. Application of Sulphur @ 40 kg ha-1 recorded maximum plant
height (47.31 cm), number of leaves plant-1 (49.80), number of nodules
plant-1 (25.58), haulm yield (28.80 q ha-1), grain yield (7.92 q ha-1) and
phosphorus, sulphur and protein content (0.295 , 0.281 and 21.79%,
respectively). Inoculation of blackgram seeds with phosphorus
solubilizing bacteria recorded slightly higher grain yield (7.49 q ha-1) as
compared to no inoculation (7.39 q ha-1).
Key words: Blackgram, phosphorus, sulphur, PSB, nutrient content
INTRODUCTION
Blackgram (Vigna mungo) is one of the important pulse crops grown
throughout India. Proper fertilization is essential to improve the productivity of
blackgram. It can meet its nitrogen requirements by symbiotic fixation of
atmospheric nitrogen. The nutrients which need attention are phosphorus and sulphur
1 Corresponding author email: aamir_sab@yahoo.com
Received: 03.06.2012
2 A. H. Mir et al.
(Thakur and Negi, 1985; Nandal, et al., 1987). Blackgram is very much responsive to
sulphur application (Aulakh, et at., 1997). Both phosphorus and sulphur can improve
the quality and quantity of the crop. Hence, the present investigation was undertaken
to find out the response of blackgram to different levels of phosphorus, sulphur and
PSB application.
MATERIALS AND METHODS
Field experiments were conducted at soil science research farm of Allahabad
Agricultural Institute-Deemed University, Allahabad for two consecutive years (2004
and 2005) with blackgram. The soil of the experimental plot was sandy loam having
pH 7.8, organic carbon 0.31%, and the available N, P S and K 213, 23.40, 13.55 and
230.10 kg ha-1 respectively. The experiment was carried out in a randomized block
design with three replications. The treatments consisted of three levels of phosphorus
(0, 30 and 60 kg ha-1), three levels of sulphur (0, 20 and 40 kg ha-1) with or without
PSB inoculation. The PSB culture “microphos” containing inoculums of
Pseudomonas straita was used for inoculation blackgram @ 10 g kg-1 of seed.
Growth parameters viz., plant height, number of leaves per plant and number of
nodules per plant were recorded at 20 days interval, where as yield parameters (grain
and haulm yield qha-1) were recorded at harvesting stage and averages were
calculated and statistically analysed by ANOVA technique (Fisher, 1950).
Phosphorus and sulphur content in grains was estimated by wet digestion method,
and protein content by multiplying factor 6.24 to nitrogen content.
RESULTS AND DISCUSSION
Effect on growth and yield
Growth, yield and nutrient status parameters (plant height, number of nodule,
number of leaves per plant, grain yield, haulm yield, phosphorus content, sulphur
content and protein content) of blackgram increased significantly with higher levels
of phosphorus, sulphur and PSB inoculation during both the years (Table 1). Among
different phosphorus levels, phosphorus @ 60 kg ha-1 recorded comparatively higher
growth and yield. An increase of about 59.61, 19.71, 19.49 and 12.78% in plant
height, number of leaves, grain yield and haulm yield, respectively was observed as
compared to control. Among the sulphur levels, sulphur @ 40 kg ha-1 significantly
increased the plant height (39.59%), number of leaves plant-1(15.86), grain yield q ha-
1 (13.46%) and haulm yield (10.93%) of blackgram as compared to no sulphur
application. Inoculation of seeds with PSB also showed positive response as
compared to no inoculation by producing taller plants, more number of leaves and
nodules plant-1. An increase of about 4.0, 1.77, 1.35and 1.10% in plant height,
number of leaves, grain yield and haulm yield respectively was observed with PSB
seed treatment as compared to without seed treatment .With increasing levels of
phosphorus, the response of sulphur also increased significantly. Similar results were
GROWTH, YIELD AND NUTRIENT CONTENT OF BLACKGRAM 3
observed by Aulakh and Pasrich (1978) and Arunachalam, et al., (1995). The
significant role of sulphur in increasing the growth and yield might be attributed to its
role in chlorophyll synthesis.
Phosphorus @ 60 kg ha-1 produced significantly maximum grain and haulm
yield of 8.02, 8.06 and 8.04 q ha-1 28.90, 28.96 and 28.93 q ha-1during 2004, 2005
and pooled data respectively. Sulphur application significantly influenced the grain
and haulm yield of blackgram. Maximum grain yield during 2004, 2005 and pooled
data respectively (7.90, 7.94 and 7.92 q ha-1) and haulm yield (28.77, 28.83 and 28.80
q ha-1) was observed with the application of sulphur @ 40 kg ha-1 as compared to
lower levels. The increase in yield might be due to vigorous growth which helped the
plants in more absorption of nutrients from the soil (Singh, et al., 1994). The results
were in line with those of Dubey, (1996) and Karwasara and Roy, (1984).
Nutrient content
Phosphorus and sulphur content in grains of blackgram increased with
increasing levels of phosphorus, sulphur and PSB application during both the years
and pooled data as shown in table 2. Application of Phosphorus @ 60 kg ha-1,
Sulphur @ 40 kg ha-1 and inoculation with PSB culture recorded maximum nutrient
concentration of 0.356, 0.295 and 0.301% of phosphorus content, whereas 0.253,
0.281 and 0.247% sulphur content in grains compared to lower levels and without
inoculation. Phosphorus solubilizers increased the availability thereby improved
phosphorus and sulphur nutrition of plant and uptake of nutrient manifested in
increased concentration (Trivedi, 1996). These results are in conformity with the
findings of Raut et al. (2000).
Higher sulphur content in grains with fertilizer application might be due to
higher absorption of nutrients as the pool of available nutrients increased in the soil.
Dubey et al., 1999; Stewat and Whitfield, 1985 also reported that addition of sulphur
produced plants with high content of both nitrogen and sulphur.
Protein content improved with the increasing levels of sulphur and phosphorus.
An increase of 10.27, 1.77 and 3.53% in protein content was recorded during 2004,
2005 and pooled data respectively as shown in table 2. The maximum content of
protein was recorded at the highest levels of applied nutrients. It might be due to
enhanced absorption of nitrogen, which ultimately increased the protein content in
seeds. Favourable influence of sulphur on protein content was due to synthesis of
sulphur containing amino acids and nitrogen uptake. Similar views were also
reported by Singh et al. (1992) and Kushwaha and Srivastava (1978).
Table 1: Effect of different levels of Phosphorus, Sulphur and PSB on plant height, number of leaves, number of
nodules, grain and haulm yield of blackgram
Plant height (cm) No. of leaves plant-1 No. of nodules plant-1 Grain yield (q ha-1) Haulm yield (q ha-1)
Treatment 2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled
P2O5 (kgha-1)
0 31.20 31.34 31.27 42.13 42.37 42.25 11.57 11.83 11.70 6.71 6.74 6.72 25.63 25.67 25.65
30 42.07 42.31 42.19 46.56 46.90 46.73 21.17 21.70 21.43 7.56 7.58 7.57 27.41 27.47 27.44
60 49.82 50.12 49.97 50.67 51.03 50.85 27.37 28.27 27.82 8.02 8.06 8.04 28.90 28.96 28.93
CD (P=0.05) 0.235 0.166 0.152 0.192 0.225 0.203 0.180 0.235 0.166 0.043 0.031 0.022 0.144 0.152 0.135
S (kg ha-1)
0 33.80 33.98 33.89 42.82 43.13 42.98 13.63 13.90 13.77 6.88 6.90 6.98 25.93 25.98 25.96
20 42.12 42.33 42.23 46.90 47.20 47.05 21.30 21.90 21.60 7.50 7.54 7.52 27.24 27.29 27.26
40 47.17 47.45 47.31 49.63 49.97 49.80 25.17 26.00 25.58 7.90 7.94 7.92 28.77 28.83 28.80
CD (P=0.05) 0.235 0.166 0.152 0.192 0.225 0.203 0.180 0.235 0.166 0.043 0.031 0.022 0.144 0.152 0.135
PSB
Without
PSB 40.20 40.42 40.31 46.04 46.36 46.20 19.33 19.84 19.59 7.38 7.41 7.39 27.17 27.22 27.19
With PSB 41.20 42.08 41.97 46.87 47.18 47.02 20.74 21.36 21.05 7.47 7.51 7.49 27.46 27.52 27.49
CD (P=0.05) 0.192 0.136 0.124 0.157 0.184 0.166 0.150 0.192 0.136 0.035 0.025 0.018 0.188 0.124 0.111
Table 2: Effect of Phosphorus, Sulphur and PSB on nutrient content in grains and available phosphorus in post
harvest soil
P-content in grains
(%) S-content in grains
(%) Protein content
(%) Available phosphorus in soil
(kg ha-1) Treatment
2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled
P2O5 (kg ha-1)
0 0.23 0.23 0.23 0.23 0.23 0.23 20.46 20.61 20.53 24.69 23.55 24.12
30 0.26 0.26 0.26 0.24 0.24 0.24 21.49 21.77 21.63 27.97 27.59 27.78
60 0.35 0.35 0.35 0.25 0.25 0.25 22.52 22.77 22.64 31.20 30.56 30.88
CD (P=0.05) 0.009 0.011 0.011 0.004 0.004 0.004 0.244 0.263 0.166 0.224 0.117 0.071
S (kg ha-1)
0 0.26 0.26 0.26 0.20 0.20 0.20 21.27 21.55 21.41 27.55 26.83 27.19
20 0.28 0.28 0.28 0.24 0.24 0.24 21.51 21.70 21.60 27.97 27.24 27.60
40 0.29 0.29 0.29 0.28 0.28 0.28 21.68 21.90 21.79 28.34 27.63 27.98
CD (P=0.05) 0.009 0.011 0.011 0.004 0.004 0.004 0.244 0.263 0.166 0.224 0.117 0.071
PSB
Without PSB 0.26 0.26 0.26 0.24 0.24 0.24 21.21 21.39 21.30 27.47 26.59 27.03
With PSB 0.30 0.30 0.30 0.24 0.24 0.24 21.77 22.04 21.90 28.44 27.88 28.16
CD (P=0.05) 0.008 0.008 0.008 0.003 0.003 0.003 0.203 0.214 0.136 0.183 0.095 0.055
6 A. H. Mir et al.
REFERENCES
Arunachalam, L., Purushothaman, S., Palniappan, S.P. and Mark Devesagayam, M. 1995.
Response of pigeon pea to phosphorus and sulphur nutrition. Madras Agricultural
Journal. 82: 266-267.
Aulakh, M. S and Pasricha, N. S.1978. Interaction effect of sulphur and phosphorus on
growth and nutrient content of moong. Plant and Soil, 47: 341-350.
Dubey, S. K .1996. Response of rock phosphate applied with Pseudomonas straita in a
typical chromustert. Journal of Indian Society of Soil Science, 44 (2): 252-255.
Dubey, Y. P; Kalstha, B. P and Jaggi, R. C.1999. Influence of irrigation and phosphorus on
growth, green pod yield and nutrient uptake of pea (Pisum sativum) in Lahaul valley of
H.P. Indian journal of Agronomy, 44: 137-140.
Fisher, R. A.1950. Handbook of Agricultural Statistics. Achal Prakashan Mandir, pp. 332-
334.
Kushwaha, P. D and Srivastava, G. P .1978. Effect of nitrogen, phosphorus and inoculation
on bio-chemical composition of moong grain. Indian journal of Agricultural
Chemistry, 11: 43-49.
Karwasara, S. P. S and Roy, M .1984. Yield and sulphur uptake of green gram as affected by
sources and levels of sulphur. International Journal of Tropical Agriculture,11(4): 331-
335.
Nandal, D. P; Malik, D. S and Singh, K. P .1987. Effect of phosphorus levels on Dry matter
accumulation of kharif pulses. Legume Research, 19 (1): 31-33.
Raut, R. F; Abdul Hamid; Hadole, S. S and Jeughale, G. S .2000. Effect of irrigation and
sulphur on concentration, uptake and availability of sulphur, nitrogen andphosphorus in
mustard. Journal of Soils and Crops, 10: 145-148.
Singh Pushpundra; Verma, B. S and Sahu, M. P .1994. Effect of levels and sources of
phosphorus and bio-regulators on ground nut (Arachis hypogaea L.). Indian journal of
Agronomy, 39 (1): 66-70.
Stewat, B. A and Whitfield, C. J .1985. Effect of crop residues, soil temperature and sulphur
on growth of winter wheat. Soil Science Society of American Proceedings, 29: 752-
755.
Surendra, Singh; Singh, A. P and Singh, B .1992. Direct and residual effect of pyrites on
yield, protein content and sulphur uptake by blackgram and lentil. Journal of Indian
Society of Soil Science, 40 (3): 584-585.
Thakur, R. C and Negi, S .1985. Effect of fertilizers and rhizobium inoculation in blackgram.
Indian journal of Agronomy, 30 (4): 501-504.
Trivedi, S. K .1996. Response of blackgram (Phaseolus mungo) to nitrogen, phosphorus and
sulphur. Legume Research, 19: 7-9
Trivedi, S. K and Sharma .1997. Response of soybean to nitrogen and sulphur fertilization
and fate of mustard grown on residual nutrients. Crop Research, 13 (1): 81-85