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Growth, yield and nutrient content of blackgram (Vigna mungo) as influenced by levels of phosphorus, sulphur and Phosphorus Solubilizing Bacteria

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A field experiment was conducted at Allahabad Agricultural Institute- Deemed University, Allahabad to study the effect of levels of phosphorus, sulphur and Phosphorus Solubilizing Bacteria (PSB) on growth, yield and nutrient content of blackgram for consecutive two years 2004 and 2005. The crop growth parameters viz., plant height, number of nodules and number of leaves per plant, yield and nutrient content increased significantly with the application of high levels of phosphorus, sulphur with or without bio-fertilizer inoculation. Application of 60 kg P 2 O 5 ha -1 recorded maximum plant height (49.9 cm), number of leaves plant -1 (50.8), number of nodules plant -1 (27.8), haulm yield (28.9 q ha -1 ), grain yield (8 q ha -1 ) and phosphorus, sulphur and protein content of grain (0.356 %, 0.253% and 22.64%, respectively) as compared to lower levels. Application of Sulphur @ 40 kg ha -1 recorded maximum plant height (47.31 cm), number of leaves plant -1 (49.80), number of nodules plant -1 (25.58), haulm yield (28.80 q ha -1 ), grain yield (7.92 q ha -1 ) and phosphorus, sulphur and protein content (0.295 , 0.281 and 21.79%, respectively). Inoculation of blackgram seeds with phosphorus solubilizing bacteria recorded slightly higher grain yield (7.49 q ha -1 ) as compared to no inoculation (7.39 q ha -1 ). DOI: http://dx.doi.org/10.3329/sja.v11i1.18370 SAARC J. Agri., 11(1): 1-6 (2013)
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SAARC J. Agri., 11(1): 1-6 (2013)
GROWTH, YIELD AND NUTRIENT CONTENT OF
BLACKGRAM (Vigna mungo) AS INFLUENCED BY
LEVELS OF PHOSPHORUS, SULPHUR AND
PHOSPHORUS SOLUBILIZING BACTERIA
A. H. Mir1, S. B. Lal, M. Salmani, M. Abid and I. Khan
Department of Soil & Environmental Sciences, Allahabad Agricultural Institute-Deemed University,
Allahabad, 211007, India
ABSTRACT
A field experiment was conducted at Allahabad Agricultural Institute-
Deemed University, Allahabad to study the effect of levels of
phosphorus, sulphur and Phosphorus Solubilizing Bacteria (PSB) on
growth, yield and nutrient content of blackgram for consecutive two years
2004 and 2005. The crop growth parameters viz., plant height, number of
nodules and number of leaves per plant, yield and nutrient content
increased significantly with the application of high levels of phosphorus,
sulphur with or without bio-fertilizer inoculation. Application of 60 kg P2O5
ha-1 recorded maximum plant height (49.9 cm), number of leaves plant-1
(50.8), number of nodules plant-1(27.8), haulm yield (28.9 q ha-1), grain
yield (8 q ha-1) and phosphorus, sulphur and protein content of grain
(0.356 %, 0.253% and 22.64%, respectively) as compared to lower
levels. Application of Sulphur @ 40 kg ha-1 recorded maximum plant
height (47.31 cm), number of leaves plant-1 (49.80), number of nodules
plant-1 (25.58), haulm yield (28.80 q ha-1), grain yield (7.92 q ha-1) and
phosphorus, sulphur and protein content (0.295 , 0.281 and 21.79%,
respectively). Inoculation of blackgram seeds with phosphorus
solubilizing bacteria recorded slightly higher grain yield (7.49 q ha-1) as
compared to no inoculation (7.39 q ha-1).
Key words: Blackgram, phosphorus, sulphur, PSB, nutrient content
INTRODUCTION
Blackgram (Vigna mungo) is one of the important pulse crops grown
throughout India. Proper fertilization is essential to improve the productivity of
blackgram. It can meet its nitrogen requirements by symbiotic fixation of
atmospheric nitrogen. The nutrients which need attention are phosphorus and sulphur
1 Corresponding author email: aamir_sab@yahoo.com
Received: 03.06.2012
2 A. H. Mir et al.
(Thakur and Negi, 1985; Nandal, et al., 1987). Blackgram is very much responsive to
sulphur application (Aulakh, et at., 1997). Both phosphorus and sulphur can improve
the quality and quantity of the crop. Hence, the present investigation was undertaken
to find out the response of blackgram to different levels of phosphorus, sulphur and
PSB application.
MATERIALS AND METHODS
Field experiments were conducted at soil science research farm of Allahabad
Agricultural Institute-Deemed University, Allahabad for two consecutive years (2004
and 2005) with blackgram. The soil of the experimental plot was sandy loam having
pH 7.8, organic carbon 0.31%, and the available N, P S and K 213, 23.40, 13.55 and
230.10 kg ha-1 respectively. The experiment was carried out in a randomized block
design with three replications. The treatments consisted of three levels of phosphorus
(0, 30 and 60 kg ha-1), three levels of sulphur (0, 20 and 40 kg ha-1) with or without
PSB inoculation. The PSB culture “microphos” containing inoculums of
Pseudomonas straita was used for inoculation blackgram @ 10 g kg-1 of seed.
Growth parameters viz., plant height, number of leaves per plant and number of
nodules per plant were recorded at 20 days interval, where as yield parameters (grain
and haulm yield qha-1) were recorded at harvesting stage and averages were
calculated and statistically analysed by ANOVA technique (Fisher, 1950).
Phosphorus and sulphur content in grains was estimated by wet digestion method,
and protein content by multiplying factor 6.24 to nitrogen content.
RESULTS AND DISCUSSION
Effect on growth and yield
Growth, yield and nutrient status parameters (plant height, number of nodule,
number of leaves per plant, grain yield, haulm yield, phosphorus content, sulphur
content and protein content) of blackgram increased significantly with higher levels
of phosphorus, sulphur and PSB inoculation during both the years (Table 1). Among
different phosphorus levels, phosphorus @ 60 kg ha-1 recorded comparatively higher
growth and yield. An increase of about 59.61, 19.71, 19.49 and 12.78% in plant
height, number of leaves, grain yield and haulm yield, respectively was observed as
compared to control. Among the sulphur levels, sulphur @ 40 kg ha-1 significantly
increased the plant height (39.59%), number of leaves plant-1(15.86), grain yield q ha-
1 (13.46%) and haulm yield (10.93%) of blackgram as compared to no sulphur
application. Inoculation of seeds with PSB also showed positive response as
compared to no inoculation by producing taller plants, more number of leaves and
nodules plant-1. An increase of about 4.0, 1.77, 1.35and 1.10% in plant height,
number of leaves, grain yield and haulm yield respectively was observed with PSB
seed treatment as compared to without seed treatment .With increasing levels of
phosphorus, the response of sulphur also increased significantly. Similar results were
GROWTH, YIELD AND NUTRIENT CONTENT OF BLACKGRAM 3
observed by Aulakh and Pasrich (1978) and Arunachalam, et al., (1995). The
significant role of sulphur in increasing the growth and yield might be attributed to its
role in chlorophyll synthesis.
Phosphorus @ 60 kg ha-1 produced significantly maximum grain and haulm
yield of 8.02, 8.06 and 8.04 q ha-1 28.90, 28.96 and 28.93 q ha-1during 2004, 2005
and pooled data respectively. Sulphur application significantly influenced the grain
and haulm yield of blackgram. Maximum grain yield during 2004, 2005 and pooled
data respectively (7.90, 7.94 and 7.92 q ha-1) and haulm yield (28.77, 28.83 and 28.80
q ha-1) was observed with the application of sulphur @ 40 kg ha-1 as compared to
lower levels. The increase in yield might be due to vigorous growth which helped the
plants in more absorption of nutrients from the soil (Singh, et al., 1994). The results
were in line with those of Dubey, (1996) and Karwasara and Roy, (1984).
Nutrient content
Phosphorus and sulphur content in grains of blackgram increased with
increasing levels of phosphorus, sulphur and PSB application during both the years
and pooled data as shown in table 2. Application of Phosphorus @ 60 kg ha-1,
Sulphur @ 40 kg ha-1 and inoculation with PSB culture recorded maximum nutrient
concentration of 0.356, 0.295 and 0.301% of phosphorus content, whereas 0.253,
0.281 and 0.247% sulphur content in grains compared to lower levels and without
inoculation. Phosphorus solubilizers increased the availability thereby improved
phosphorus and sulphur nutrition of plant and uptake of nutrient manifested in
increased concentration (Trivedi, 1996). These results are in conformity with the
findings of Raut et al. (2000).
Higher sulphur content in grains with fertilizer application might be due to
higher absorption of nutrients as the pool of available nutrients increased in the soil.
Dubey et al., 1999; Stewat and Whitfield, 1985 also reported that addition of sulphur
produced plants with high content of both nitrogen and sulphur.
Protein content improved with the increasing levels of sulphur and phosphorus.
An increase of 10.27, 1.77 and 3.53% in protein content was recorded during 2004,
2005 and pooled data respectively as shown in table 2. The maximum content of
protein was recorded at the highest levels of applied nutrients. It might be due to
enhanced absorption of nitrogen, which ultimately increased the protein content in
seeds. Favourable influence of sulphur on protein content was due to synthesis of
sulphur containing amino acids and nitrogen uptake. Similar views were also
reported by Singh et al. (1992) and Kushwaha and Srivastava (1978).
Table 1: Effect of different levels of Phosphorus, Sulphur and PSB on plant height, number of leaves, number of
nodules, grain and haulm yield of blackgram
Plant height (cm) No. of leaves plant-1 No. of nodules plant-1 Grain yield (q ha-1) Haulm yield (q ha-1)
Treatment 2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled
P2O5 (kgha-1)
0 31.20 31.34 31.27 42.13 42.37 42.25 11.57 11.83 11.70 6.71 6.74 6.72 25.63 25.67 25.65
30 42.07 42.31 42.19 46.56 46.90 46.73 21.17 21.70 21.43 7.56 7.58 7.57 27.41 27.47 27.44
60 49.82 50.12 49.97 50.67 51.03 50.85 27.37 28.27 27.82 8.02 8.06 8.04 28.90 28.96 28.93
CD (P=0.05) 0.235 0.166 0.152 0.192 0.225 0.203 0.180 0.235 0.166 0.043 0.031 0.022 0.144 0.152 0.135
S (kg ha-1)
0 33.80 33.98 33.89 42.82 43.13 42.98 13.63 13.90 13.77 6.88 6.90 6.98 25.93 25.98 25.96
20 42.12 42.33 42.23 46.90 47.20 47.05 21.30 21.90 21.60 7.50 7.54 7.52 27.24 27.29 27.26
40 47.17 47.45 47.31 49.63 49.97 49.80 25.17 26.00 25.58 7.90 7.94 7.92 28.77 28.83 28.80
CD (P=0.05) 0.235 0.166 0.152 0.192 0.225 0.203 0.180 0.235 0.166 0.043 0.031 0.022 0.144 0.152 0.135
PSB
Without
PSB 40.20 40.42 40.31 46.04 46.36 46.20 19.33 19.84 19.59 7.38 7.41 7.39 27.17 27.22 27.19
With PSB 41.20 42.08 41.97 46.87 47.18 47.02 20.74 21.36 21.05 7.47 7.51 7.49 27.46 27.52 27.49
CD (P=0.05) 0.192 0.136 0.124 0.157 0.184 0.166 0.150 0.192 0.136 0.035 0.025 0.018 0.188 0.124 0.111
Table 2: Effect of Phosphorus, Sulphur and PSB on nutrient content in grains and available phosphorus in post
harvest soil
P-content in grains
(%) S-content in grains
(%) Protein content
(%) Available phosphorus in soil
(kg ha-1) Treatment
2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled 2004 2005 Pooled
P2O5 (kg ha-1)
0 0.23 0.23 0.23 0.23 0.23 0.23 20.46 20.61 20.53 24.69 23.55 24.12
30 0.26 0.26 0.26 0.24 0.24 0.24 21.49 21.77 21.63 27.97 27.59 27.78
60 0.35 0.35 0.35 0.25 0.25 0.25 22.52 22.77 22.64 31.20 30.56 30.88
CD (P=0.05) 0.009 0.011 0.011 0.004 0.004 0.004 0.244 0.263 0.166 0.224 0.117 0.071
S (kg ha-1)
0 0.26 0.26 0.26 0.20 0.20 0.20 21.27 21.55 21.41 27.55 26.83 27.19
20 0.28 0.28 0.28 0.24 0.24 0.24 21.51 21.70 21.60 27.97 27.24 27.60
40 0.29 0.29 0.29 0.28 0.28 0.28 21.68 21.90 21.79 28.34 27.63 27.98
CD (P=0.05) 0.009 0.011 0.011 0.004 0.004 0.004 0.244 0.263 0.166 0.224 0.117 0.071
PSB
Without PSB 0.26 0.26 0.26 0.24 0.24 0.24 21.21 21.39 21.30 27.47 26.59 27.03
With PSB 0.30 0.30 0.30 0.24 0.24 0.24 21.77 22.04 21.90 28.44 27.88 28.16
CD (P=0.05) 0.008 0.008 0.008 0.003 0.003 0.003 0.203 0.214 0.136 0.183 0.095 0.055
6 A. H. Mir et al.
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... However, all the treatment was statistically at par (pooled data), except during 2018, where T 7 , T 6 and T 4 was statistically different from other treatments. Higher groundnut pod yield in plots receiving higher dose of fertilizer was possibly due to superior growth responses and yield-determining factors (Mir et al. 2014). While, interactions between mineral P fertilizer and PSB inoculant reported higher yield, sole use of microbial culture or with FYM did not improve yield of peanut (Argaw 2016). ...
Article
Present study was carried out for three consecutive kharif season of 2018, 2019 and 2020 to evaluate the performance of different levels of chemical phosphatic fertilizers and FYM in combination with PSB inoculant on groundnut growth, yield, quality and nutrient uptake in an acidic Red and Laterite soil. Application of higher dose of phosphatic fertilizer along with PSB inoculant yielded better results with respect to groundnut plant height, nodulation, pegs and pods plant À1 , weight of mature pods plant À1 , kernel and haulm yield, protein and oil content, and nutrient uptake. Treatment combination T7 (60 kg ha À1 of P2O5 + PSB inoculant) was observed as the best treatment combination for deriving maximum growth, yield and nutrients uptake of kharif groundnut crop. Treatment T6 (application of 40 kg ha À1 of P2O5 + PSB inoculant) reported similar results. However, maximum profit ($862), benefit cost ratio (2.7) and net return per unit cost of input was significantly higher in T7 compared to T6. Thus, we recommend application of 60 kg ha À1 of P2O5 with PSB inoculant (T7) for the light textured acid soils of Red and Laterite zone of India for higher production and profitability.
... The plant height and number of branches plant -1 could be more because of the established role that sulphur plays in promoting cell division, the process of photosynthesis, and the creation of chlorophyll. Similar findings were reported by Mir et al., [8] and Sivakumar et al., [9]. It also encourages the development of the plant root nodules, which make more sulphur available during the vegetative growth cycle. ...
Article
Aim: To study the effect of soil application and foliar spray of sulphur nutrient application in growth and development of black gram cultivation. Study Design: The Factorial Randomized Block Design (FRBD) was used. The treatments of soil application of sulphur viz., No basal, Gypsum @ 25 kg ha-1, SOB @ 2 kg ha-1 as basal and foliar spray viz., No spray, pulse wonder @ 5 kg ha-1on 30 DAS, K2SO4 @ 1% on 45 DAS, pulse wonder @ 5 kg ha-1 on 30 DAS + K2SO4 @ 1% on 45 DAS were used. Place and Duration of Study: This research trail was conducted during the Winter season (February – May) of 2022 at ICAR - Krishi Vigyan Kendra, Sirugamani, Trichy, Tamil Nadu, India. Methodology: The study consisted of 12 combination of treatments in FRBD which was replicated thrice. The black gram (Var. Vamban 8) was used for this study. The observations were recorded during the different phases of growth at regular intervals. Results: The experimental results revealed that the interaction effect of application of gypsum as basal along with foliar spray of pulse wonder + K2SO4 found to be superior and recorded significantly higher growth, yield and quality parameters viz., plant height, number of branches plant-1, dry matter accumulation, SPAD value at 20 DAS, 40 DAS and 60 DAS number of pods plant-1, number of seed pod-1, pod length, pod weight and sulphur content in seed. Gypsum as basal application along with foliar spray of pulse wonder + K2SO4 proved to be the best and increased the grain yield of 30% over the no basal application and no foliar spray. Thus application of gypsum @ 25 kg ha-1 as basal and foliar spray of pulse wonder @ 5 kg ha-1 on 30 DAS + K2SO4 @ 1% on 45 DAS could be recommended for improving the productivity of black gram.
... This may be due to increase in plant height may due to stimulation of biological activities in the presence of balanced supply of phosphorus. Similar results on the increased plant height with increased levels of phosphorus application have been reported by "Phosphorus encourage formation of new cells, promote plant vigour and hastens leaf development, which help in harvesting more solar energy and better utilization of nitrogen, which help towards higher growth attributes" Mir et al. [14]. Increase in plant height might be the involvement of boron in different physiological processes like enzyme activation, electron transport, chlorophyll formation, stomatal regulation, etc. ...
Article
A field experiment was conducted during Rabi season (2021) at Crop Research Farm, Department of Agronomy, SHUATS, Prayagraj (U.P.). The soil of experimental plot was sandy loam in texture, nearly natural in soil reaction (pH 7.1), low in organic carbon (0.28%), available Nitrogen (225 kg/ha), available Phosphorous (19.50 kg/ha) and available potassium (213.7 kg/ha). The Treatments consisted of 3 levels of Phosphorous (Phosphorous -30 kg/ha), (Phosphorous -45 kg/ha) (Phosphorous -60 kg/ha) and 3 levels of Boron (Boron -1 kg/ha), (Boron -1.5 kg/ha) and (Boron - 2 kg/ha). The experiment was laid out in Randomized Block Design with 9 treatments and replicated thrice. The results reported that significantly maximum plant height (53.50 cm), dry weight (8.26 g/plant) and a greater number of nodules (7.80) were found higher with application of treatment 60 kg/ha phosphorous + 2 kg/ha Boron. No. pods/plant (103.70), seeds/pod (2.00), test weight (24.38 g), grain yield (1837.72 kg/ha) and Stover yield (2906.16 kg/ha) were recorded in (treatment-9) that is with 60 kg/ha phosphorous + 2 kg/ha Boron.
Article
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Sulphur-phosphorus interaction has been studied in a greenhouse experiment on a soil, deficient both in S and P with moong (Phaseolus aureus) as the test crop. The treatments were in the factorial combination of five levels of S and P (0, 5, 10, 20 and 40 ppm) applied as CaSO4. 2H2O and Ca(H2PO4)2. H2O respectively. A uniform treatment of N and K was made. The yield of vegetative tissues and grains increased with the application of S and P individually but decreased when S and P were applied in different combinations. Sulphur application increased S content but decreased P content in straw as well as in grains. Total P content increased with applied P and decreased with S application. Applied sulphur increased and phosphorus decreased the protein content in moong grains. Changes in N:S ratio in interaction was found to exist between S and P on the yield, grain quality, concentration and total removal of sulphur and phosphorus by Moong crop The antagonistic effect of sulphur and phosphorus fertilizer on the uptake and utilization of each other was more conspicuous when they were applied together. re]19760106
Article
A field experiment was conducted to study the effect of irrigation and phosphorus levels on pea (Pisum sativum L s.l.) at Kukumseri in Lahaul Valley of Himachal Pradesh, during summer of 1993 and 1994. The individual as well as cumulative response of irrigation and P was found significant. Phosphorus @ 80, 40 and 0 kg P2O5/ha in different irrigation and P level combinations gave maximum green-pod yields at 4, 8 and 12 days interval of irrigation respectively.
Yield and sulphur uptake of green gram as affected by sources and levels of sulphur
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