This study explored the role of transglottal pressure on the lowering of vocal fundamental frequency (Fo-lowering) during voiced plosive/b/ production. The subjects were 3 males and 3 females. Magnitudes of Fo-lowering were recorded under two different conditions: 1) when the vocal tract was abruptly interrupted during sustained phonation, and 2) when/i: bi: /was uttered. There was no consistent relation between the magnitudes of Fo-lowerings obtained under these two different conditions. In other words, Fo-lowering during /b/ production could not be explained by changes of transglottal pressure only. This implyies positive participation of the laryngeal muscles in the mechanism of Fo-lowering. © 1993, The Japan Society of Logopedics and Phoniatrics. All rights reserved.