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THE EXAMINATION OF UNPACKAGED ICE-CREAM RETAILED SAMPLES IN ISTANBUL CITY ASPECT OF COAGULASE POSITIVE STAPHYLOCOCCUS AUREUS

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ABSTRACT
THE EXAMINATION OF UNPACKAGED ICE-CREAM RETAILED SAMPLES IN
ISTANBUL CITY ASPECT OF COAGULASE POSITIVE STAPHYLOCOCCUS
AUREUS
The aim of this study was to evaluate the incidence of Staphylococcus aureus in unpackaged
ice cream retailed samples in Istanbul. For that purpose total of 101 unpackaged ice-cream
samples which were collected from different manufactures in district of Istanbul where are
Beykoz, Umraniye, Uskudar, Sariyer and Besiktas between August 2015 to Semptember
2015. Isolates tested for the DNase, catalase, coagulase enzyme activities and methicillin
resistance properties.
Staphylococcus aureus is determined in 66 (65,3%) of total 101 samples using cultural
method. S. aureus contamination rate of 35(34,6%) out of 101 ice-cream samples exceeded
the corresponding Turkish Food Codex (anounement number 2009/68, numbered 27456)
maximum limits of Microbiological Criteria Anouncement.
Totally 94 of Staphylococcus aureus strains were isolated from the ice cream samples and it
was found that 61 (64,8%) of the strains were coagulase positive, 81 of them (86,1%) were
catalase positive, 21 of them (22,3%) were DNase positive and 18 of them (19,1%) had
methicillin resistance.
The results of this study indicate that unpackaged ice-cream sold at retail in different districts
of Istanbul doesn’t have adequate microbiologicial criterias. Ice-creams which are commonly
consumed especially in children, produced inadequate microbiological conditions and
contaminated may poses serious risks in terms of food safety and public health. Therefore it
was concluded that necessary hygienic measures especially in terms of raw material, personal
hygiene, and disinfection of equipements in the icecream production stages should be taken
and HACCP, GMP and GHP regulations should be implemented.
Key Words: Ice-cream, Staphylococcus aureus, coagulase,catalase, DNase, Meticillin
resistance.
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