Plant tissue culture is one of most important part of biotechnology. Terminalia arjuna is a widely used medicinal plant throughout India and popular in various indigenous system in Ayurveda, Sidha, Unani. Indian system of medicine bark is used as astringent cooling, cardiotonic, tonic in fracture ulcer , spermatorrhoea, diabetes, cough, tumour, excessive perspiration asthama inflamation and skin ... [Show full abstract] disorder. In order to achieve callus induction from leaf of Terminalia arjuna different hormonal combination were tried. Mature leaf did not respond well; however, juvenile leaf gave good response. Out of various media combination tried, the explant showed callus induction on supplemented medium(MS media) contains1mg/litre NAA along with 1mg/litre BAP .