Usefulness of a nutraceutical diet to improve qol and drugs use in a dog
affected by a mast cell tumor: a case report.
Paolo Guazzi1, Sergio Canello2, Gianandrea Guidetti3 , Alessandro Di Cerbo4*
1Wiligelmo Veterinary Clinic, Modena, Italy
2Research and Development Department, USA, Orlando, USA
3Research and Development Department, Padua, Italy, Modena, Italy
4Department of Medical, Oral and Biotechnological Sciences, Dental School, University G. D`Annunzio of Chieti-
Pescara, Chieti, Italy
Accepted on February 06, 2017
On October 2014, a 10-years-old male neutered boxer
presented to the Wiligelmo Veterinary Clinic of Modena (Italy)
for the sudden onset of 3 cutaneous nodular lesions (Figure 1).
The first, a large movable lesion, was clearly visible on the
dorsal face of the second finger of the right forepaw (Figures
1A and 1B), which was linked to the paw by means of a tight
peduncle. Intriguingly the skin of this lesion resulted intact,
neither alopecic nor with signs of dermatitis. The second
lesion, on right side of the tail, was subcutaneous, poorly
movable and with a blackish, alopecic but not ulcerated skin
(Figure 1C). The same features characterized the third lesion on
the left dorsal side of the gluteus region (Figure 1D).
Figure 1. Graphical representation of mast cell tumor in
different cutaneous areas. (A-B) Interdigital lesion on the
dorsal face of the second finger of the right forepaw (6 cm of
length); (C) second lesion on right side of the tail (4.5 cm of
length); (D) third lesion on the left dorsal side of the gluteus
region (5 cm of length).
All lymph nodes resulted in their normal dimensions. The skin
presented some areas where the fur was easily detachable, dry,
opaque and dispersed. Food history revealed the assumption of
specific commercially available diets for gastrointestinal and
Body of the Case
The dog resulted overweighed after a general objective exam
(BCS evaluation 7/9). The cytological examinations of the 3
lesions revealed the presence of a well-differentiated mast cell
Trichological exam showed the presence of fungal spores
compatible with Microsporum gypseum. Conversely, hemato-
biochemical analyses, radiographic and ultrasonography
investigations do not showed any significant alteration. Also
liver and spleen samplings, by mean of fine needle aspiration
biopsy, as well as exon 11 analysis do not revealed any mast
cell infiltrates or genetic mutations, respectively.
Firstly the dog was assigned to receive Griseofulvin for one
month, then a dose of 1 mg/kg Prednisolone and a tablet of
Epato 1500 (twice a day). After one month the amount of
Prednisolone was reduced to 0.75 mg/kg while the Griseofulvin
was interrupted. The most difficult therapeutic concern was the
necessity to use Prednisolone, as the only drug accepted by the
owners against mast cell tumor, and the need to avoid it in
order to prevent dermatophytosis spread. Three months later a
nutraceutical, immune modulating diet (IMMUNOACTIVE,
SANYpet S.p.A) was introduced [1-3]. One week later the
Prednisolone was reduced to 0.6 mg/kg and, after 15 days since
the beginning of the nutraceutical diet supplementation, was
interrupted. Three weeks later neoplastic lesions resulted
slightly reduced (Figure 2).
Figure 2. Graphical representation of mast cell tumor in
different cutaneous areas following 2 months of dietary
intervention. (A-B) Interdigital lesion on the dorsal face of the
second finger of the right forepaw (4 cm of length); (C) second
lesion on right side of the tail (4 cm of length); (D) third lesion
on the left dorsal side of the gluteus region (3 cm of length).
Case Report http://www.alliedacademies.org/veterinary-medicine-and-allied-science/
J Vet Med Allied Sci 2017 Volume 1 Issue 1
Only itraconazole was continued every other week. After one
month the right hind knee became aching and swollen with a
II/III degree of lameness. Radiographically, the only visible
alterations were ascribed to the presence of severe arthritis
process and ligament lesions; therefore Previcox 227, once a
day, was introduced.
Twenty days later the Previcox 227 dosage was reduced to
maintenance dosage, along with a reduction of the aching,
swollen and lameness, and completely interrupted after two
months. After one month since Previcox 227 interruption a
mycosis relapse occurred and itraconazole was re-introduced.
All neoplastic lesions resulted still stable.
Seven months later an increase in all three cutaneous tumors
was observable while no mycosis was present. Despite the
daily assumption of the nutraceutical diet, the dog resumed the
Prednisolone (1 mg/kg once a day) along with Kc Omega (2
capsules a day). Four months later the dog came to the clinic
with a severe dyspnea, a moderate pleural effusion but a severe
pneumothorax. Ultrasound investigation revealed a great
hepatic mass. Due to the severe dyspnotic picture and the
diffused metastases the animal was euthanized.
Starting from a Stage III mast cell tumor, poorly responsive to
drugs belonging to the class of tyrosine kinase inhibitors,
regardless surgical treatment and other chemotherapy protocols
(refused by the owner), the choice of Prednisolone was the
only therapeutic possibility towards such tumor. Unfortunately,
this treatment was in contrast with that for dermatomycosis,
which is facilitated by cortison-like drugs both locally and
systemically . Therefore it was necessary an alternative
treatment that allowed reducing or even suspending the amount
of Prednisolone. A further difficulty was represented also by
the food hypersensitivity noticed during the anamnesis. The
use of a hypoallergenic nutraceutical diet, enriched with
botanicals (Cucumis melo, Aloe vera, Punica granatum, Piper
nigrum, Camellia sinensis, Ascophyllum nodosum, Grifola
frondosa, Glycine max, Echinacea purpurea, Poligonum spp.,
Carica papaya and Curcuma longa) with immune modulating
and anti-inflammatory activity  and a high omega 3 and 6
ratio (3:1) resulted to be a valuable support to the
pharmacological treatment, stabilizing the disease status for 11
months without the use of Prednisolone and allowing a better
response to dermatomycosis.
Both in human and veterinary medicine, recent literature
highlighted the influence of polyunsaturated fatty acids and
some phytotherapic compounds-based remedies in the
prevention and treatment of cancer [5,6]. For instance,
omega-3 polyunsaturated fatty acids, docosahexaenoic and
eicosapentaenoic acid, showed to induce apoptosis and
autophagy in MCF-7 breast cancer cells . As concerns
Curcuma longa , Aloe vera , Piper nigrum , Punica
granatum , Camellia sinensis , Ascophyllum nodosum
, Grifola frondosa , Glycine max , Echinacea
purpurea , Poligonum cuspidatum  and Carica papaya
 they all showed cytotoxic and anti-proliferative activity
towards several cancer models both in vivo and in vitro.
Finally, it is of great relevance to remind that poor body
condition score and weight loss have been related to bad
prognosis in dogs affected by neoplastic condition , and
many owner s have great interest in change the diet of their pet,
in order to help it in the every-day life, at home, using food as
a simple, but very important tool to maintain a good QOL.
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Citation: Di Cerbo A, Guazzi P, Canello S and Guidetti G. Usefulness of a nutraceutical diet to improve qol and drugs use in a dog affected by
a mast cell tumor: a case report.. J Vet Med Allied Sci 2017;1(1):1-4.
J Vet Med Allied Sci 2017 Volume 1 Issue 1
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Di Cerbo A
Department of Medical, Oral and Biotechnological Sciences,
Dental School, University G. D`Annunzio of Chieti-Pescara,
Di Cerbo, Guazzi, Canello, Guidetti
J Vet Med Allied Sci 2017 Volume 1 Issue 1