A number of mycotoxins resist rumen degradation, causing distinct clinical signs of intoxication. Most mycotoxins are chemically stable so they tend to remain potent during storage and processing, and even high temperatures, such as those reached during baking bread or breakfast cereal production. The choice of extraction solvent is dependent on the matrix from that the extraction is required, as the differing chemical mixtures can affect it. Liquid–liquid extraction (LLE) involves exploiting the different solubility of the toxin in aqueous phase and in immiscible organic phase, to extract the compound into one solvent leaving the rest of the matrix in the other. Supercritical fluid extraction (SFE) uses supercritical fluid, such as carbon dioxide (CO2) to extract the required compound from the matrix. Thin layer chromatography (TLC) is a popular method used for both quantitative and semi-quantitative mycotoxins analysis. The study developed a quantitative Monte Carlo exposure assessment model for mycotoxins in dairy milk, and assessed the potential human exposure levels.