Environmental decision-makers and practitioners need and deserve high quality environmental evidence for effective decision-making. We collate and share a suite of best practices for applied environmental researchers to support their capacity to inform such decision-making processes. This raises a number of important questions: What does “relevant” and informative evidence look like? How do we know when evidence has been applied? We assembled an experienced team of knowledge generators and users in Canada to identify insights that have emerged from their work and that could serve as guideposts for others who seek to apply environmental research to policy challenges. By reflecting on successes and failures, we define “success” in applied environmental science as respectfully conducted, partner-relevant research that is accessible, understandable, and shared, and that can create opportunities for change (e.g., in policy, behaviour, management). Next, we generated a list of best practices for delivering “successful” applied environmental research. Our guidance emphasizes the importance of engaging early and often, in a respectful manner, with partners, generating high-quality, relevant research (which requires flexibility), having a plan for communicating and sharing outputs, and being transparent about uncertainties and limitations. Other important considerations include acknowledging partners for involvement and training early career researchers in applied partnership research. Finally, we generated a list of specific, measurable indicators for evaluating success including: quality and quantity of scientific outputs, the relationship with the partner(s), relevance and connectedness of the research, accessibility and availability of outputs to users, provision of outputs that are digestible and usable by different audiences, training and capacity building, and ultimate outcomes (e.g., including social, environmental, and economic outcomes, as well as partner satisfaction). We encourage those embarking on applied environmental research to consider embracing the strategies, to continuously reflect on progress toward shared research goals, and to be flexible. Doing so will increase the likelihood of delivering research that is “successful” and in doing so contribute to overcoming and addressing environmental issues and problems.