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Abstract

Celery is recently the subject of various studies due to its role in human nutrition and for medicine purposes. One of the most speculated ideas is that celery contains high quantities of androsterone which makes it suitable for infertility treatments. Due to this trend, the purpose of this study was to develop an analytical method suitable to confirm and measure the quantity of androsterone from celery root. A LC-MS/MS method was developed using a Turbo V source in positive ionization mode. Analytical parameters such as: linearity, detection and quantification limits, accuracy and precision and matrix effect were evaluated. The calibration curve was developed in the range of 100 to 400 ng/ml with a correlation coefficient r² of 0.9968 and detection limit of 10 ng/ml. The extraction method was tested for the recovery degree. The recovery obtained was 92.1±2.2%. The method was used to determine the content of androsterone from three celery varieties from Romanian market.

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The Western Herbal Tradition provides a comprehensive and critical exploration of the use of plant medicines through 2000 years of history from Dioscorides to the present day. It follows each of the 27 herbs through a wide range of key sources from European, Arabic and American traditions including Greek, Roman and Renaissance texts. A rich discussion of the historical texts is balanced with current application and research. The herbs have been selected on the basis of common use by practising herbalists. Each illustrated monograph contains: Species, identification and botanical description A study of the characterisation and medicinal use of the plants consistently drawn from featured herbals which includes the authorsâown translations from the Latin Assessment of past and current texts in the transmission of herbal knowledge Consideration of traditional therapeutics, including humoral and physiomedical approaches Suggestions towards a modern experiential approach through Goethean methodology Current evidence on pharmacological constituents Review of evidence on safety Recommendations for internal and external uses, prescribing and dosage.
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Celery is a commercially important seed spice belonging to the family Umbelliferrae. Celery is used in various forms such as fresh herb, stalk, seeds, oil, and oleoresin for flavoring of foods and for medicinal purposes. Celery seed contains 2% volatile oil that finds application for flavoring of foods and also in perfumery industry. Limonene and selinene form about 60% and 20% of the oil, respectively. However, the important flavor constituents of the oil responsible for the typical aroma are 3-n-butyl-4-5-dihydrophthalide (sedanenolide), 3-n-butyl phthalide, sedanolide, and sedanonic anhydride present in very low levels (1-3%). Celery contains 15% fatty oil with the fatty acids: petroselenic (64.3%), oleic (8.1%), linoleic (18%), linolenic (0.6%), and palmitic acids. Phthalides especially sedanenaloide possess many health benefits. Celery extracts are reported to possess many nutraceutical properties, viz., antioxidant, hypolipidemic, hypoglycemic, and anti-platelet aggregation. In the present review, the chemistry, processing, and biological activities of celery and the components responsible are discussed.
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High-performance liquid chromatography coupled by an electrospray ion source to a tandem mass spectrometer (HPLC-ESI-MS/MS) is the current analytical method of choice for quantitation of analytes in biological matrices. With HPLC-ESI-MS/MS having the characteristics of high selectivity, sensitivity, and throughput, this technology is being increasingly used in the clinical laboratory. An important issue to be addressed in method development, validation, and routine use of HPLC-ESI-MS/MS is matrix effects. Matrix effects are the alteration of ionization efficiency by the presence of coeluting substances. These effects are unseen in the chromatogram but have deleterious impact on methods accuracy and sensitivity. The two common ways to assess matrix effects are either by the postextraction addition method or the postcolumn infusion method. To remove or minimize matrix effects, modification to the sample extraction methodology and improved chromatographic separation must be performed. These two parameters are linked together and form the basis of developing a successful and robust quantitative HPLC-ESI-MS/MS method. Due to the heterogenous nature of the population being studied, the variability of a method must be assessed in samples taken from a variety of subjects. In this paper, the major aspects of matrix effects are discussed with an approach to address matrix effects during method validation proposed.
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