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Ilex guayusa Loes (Aquifoliaceae) is native to the Andean Amazon (Colombia, Ecuador, and Perù) commonly known as guayusa. From ancestral times up to today Guayusa has been employed by indigenous and urban communities as a herbal infusion, for the treatment of diabetes, infertility, or venereal diseases. As an antiinflammatory, diuretic or energizing-agent. In addition, it can be used as a regulator of the menstrual cycle and during the lactation period. Other benefits include for weight loss, and as a mouth wash, among others. This study encompasses Ilex guayusa taxonomy, etnobotany, geographical distribution and habitat (elevation), ecology, phytochemistry, biological activity, and toxicity. Few investigations have been devoted to its phytochemical and pharmacological properties, thus other studies could suggest new medicinal effects for future alternative medicinal development.
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Archives • 2016 • vol.3 • 193-202
Sequeda-Castañeda, L.G. 1,2*; Modesti Costa, G.1; Celis, C.1; Gamboa, F.3,4; Gutiérrez, S.4; Luengas, P.2
1 Departamento de Química, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá-Colombia.
2 Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá-Colombia.
3 Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá-Colombia.
4 Centro de Investigaciones Odontológicas, Facultad de Odontología, Pontificia Universidad Javeriana, Bogotá-Colombia.
Ilex guayusa Loes (Aquifoliaceae) is native to the Andean Amazon (Colombia, Ecuador, and Perù) commonly
known as guayusa. From ancestral times up to today Guayusa has been employed by indigenous and urban
communities as a herbal infusion, for the treatment of diabetes, infertility, or venereal diseases. As an
antiinflammatory, diuretic or energizing-agent. In addition, it can be used as a regulator of the menstrual
cycle and during the lactation period. Other benefits include for weight loss, and as a mouth wash, among
others. This study encompasses Ilex guayusa taxonomy, etnobotany, geographical distribution and habitat
(elevation), ecology, phytochemistry, biological activity, and toxicity. Few investigations have been devoted
to its phytochemical and pharmacological properties, thus other studies could suggest new medicinal effects
for future alternative medicinal development.
Key words: Ilex guayusa, taxonomy, biogeography, ecology, ethnobotany, phytochemistry, biological activity, medicinal plant, toxicity.
ISSN: 1827-8620
December 30, 2016
The Andean Amazon is located in the countries of
Colombia, Ecuador, Perù, and Bolivia, encompasing
approximately three fourths of Peru and Bolivia
territory, one half of Ecuador, and one third of
Colombia. The Andean Amazon is characterizaed by
its richness in biodiversity for food, medicine,
cosmetic, and raw matrial for industry production
(1). Ilex guayusa is among the many plant species in
this region, also known as guayusa, guañusa,
huayusa, aguayusa, and wuayusa (2). For centuries
aborigens in this region have employed Ilex guayusa
as a diuretic, hypoglycemic agent, stimulant and in
ritual ceremonies, among others (3). Ilex guayusa
production has increased in the Andean Amazon
zone in the past years aiming to export, as well as
intoduce it to other countries due to its medicinal
and stimulant properties (4-6). Other medicinal
attributes include aiding in scar formation and as a
diaphoretic. It can be used to treat asthma or as an
expectorant. Its antiinflammatory properties are
kwown, thus it can be used against rheumatism. It
can also be employed as a mouth wash, against
gastritis, as an emetic, digestive, and diuretic. It is
known to reduce head and body aches, for
muscular pain, and to treat flu symptoms. Among
its various uses are as an emmenagogue, during the
lactation period, to treat venereal diseases, for
dissyness, and weight loss. Furthermore, it can be
consumed as a herbal tea. It is a blood fortifying
agent, blood pressure regulator with hypoglegymic
and antioxidant properties. Other beneficial effects
include fatigue suppressant, provides physical and
mental agility, stimulant, hallucinogen, tonner,
energizing, restorative, and aphrodisiac. Last, it aids
in the sense of awareness throughout the whole
body due to its content of a mix of theophylline,
theobromine, and caffeine (7-18).
The methods utilized to search, gather and analyze
information include the following Data Banks:
Plantlist, Scopus, PubMed, IsiWeb, Sprink link,
Francis & Taylor, SIB Bioinformatics Resource
Portal, and Sinab. Books and articles referring Ilex
guayusa ethnobotanical aspects or any subject
related to taxonomy or phytochemistry were
included. In addition, books and articles describing
biological activity, medicinal properties and toxicity
among others were also employed. The collection
of information was carried out between January
2012 and June of 2016.
Ilex guayusa Loes, Nova Acta Acad. Caes. Leop.-Carol.
German. Nat. Cur. 78:310.1901. The plant belongs to
the kingdom Plantae, Phylum: Magnoliophyta, Class:
Magnoliopsida, Order: Celastrales, Family:
Aquifoliaceae, Genus: Ilex, Species: Ilex guayusa
Loes. The tree can grow between four to 15 m
height, with a ramified trunk up to 1 m diameter. It
has dentate oblong/elliptic olive green coriaceous
leaves, glabrous or subglabrous at the blade as well
as the back of the leaf. The leaves are arranged in a
simple and alternate manner. It has an acuminate
apex with an acute base. The leaves can grow
between 15 - 21 cm long and 5 - 8 cm wide, with a
short 1 cm petiole.
Flowers have a persistent calyx and the petals
forming the corolla are obtuse. The number of
stamens are the same as for the petals, with oblong
anthers. The ovary sessile, subglose usually 4-6
celled (locules). The fruit is a globose green berry
almost 1 cm wide (3, 19-21). The plant is classified
under voucher No. HPUJ 011734 at the Pontificia
Universidad Javeriana herbarium. In addition, the
National Colombian Herbarium and Bogota Botanical
Garden Herbarium have classified this species under
vouchers COL 523700 and JBB 10344, respectively.
Geographical distribution
Ilex guayusa is a plant native to the Neotropics, with
natural distribution in Colombia, Ecuador, Peru, and
Bolivia (22, 23). According to specimens deposited in
the Colombian National Herbarium and the
Herbarium at the Universidad de El Valle, this plant is
found in the Departments of Nariño and Putumayo,
from Mocoa to Sibundoy (20, 24), in the area
between the Departments of Putumayo and Caquetá
in Colombia (25). In Ecuador this plant is found in the
provinces of Sucumbíos, Napo, Pastaza, Morona
Santiago, and Zamora Chinchipe. In addition, it has
been registered in the provinces of Pichincha and
Tungurahua (26).
In Colombia Ilex guayusa was reported in the
Department of Amazon in front of the south tip of
the Guadual Island, where it is commonly known as
detzacogque for the Miraña community.
Furthermore, in the Department of Caquetá the
Tucana indigenous community has named it
Yurugú. Moreover, it has been found in front of the
Mariname Island in poorly drained woods. Likewise,
it is found in the environmental path of Mogambo
(Figure 1), in the Municipality of Viotá, Departament
of Cundinamarca. Last, it is also established in the
National Research Center for tropical aromatic plant
species agroindustialization (Centro Nacional de
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PhOL Sequeda-Castañeda, et al 194 (193-202)
Investigaciones para la Agroindustrialización de
Especies Vegetales Aromáticas Medicinales
Tropicales: Cenivam) in Bucaramanga, Santander
Colombia (27, 28).
Altitudinal distribution
According to deposited samples in the COL
herbarium, Ilex guayusa grows in Colombia at 2,000
masl. This species is distributed in altitudes
between 200 and 2,000 masl (25). It has been
collected from Ecuador at 500 masl and Perù at 220
masl (22). Furthermore, in Ecuador this species
distribution ranges from sea level up to 1,500 masl
(26). Gupta reported this plant can grow between
200 and 350 masl (29).
Ilex guayusa is found in the Colombian lower
Neotropical jungle and in Sub-Andean forests (30).
This perennial tree is native to the Amazon region,
where it grows in the wild. However, it is also
present plantations in subtropical Andean regions
(26). This species grows in humid tropical forests in
the Colombian, Ecuadorian, and Peruvian Amazon
forming part of secondary forests (31). This plant
was reported in phytosociological association with
Tabebuia insignisMauritietum flexuosae, defined
as a vegetation unit encompassing small to medium
forests, with a short basal area, high shrub density
in the thicket, and a high palm tree percentage (32).
Ilex guayusa is a tree reported in the literature with
monoecious flowers and prone to polygamy; with
shrub like physiognomy during the juvenile stage. In
addition, it is semi-domesticated in plantations. Its
asexual reproduction strategy consists of basal
shoots, sprouts, and suckers. Phenological cycles do
not report fertile matter activity. Anthropic
distribution is limited to the Peruvian-Ecuadorian
and Colombian Amazon corridor, thus its main
biophysical requirements are the soil and water
resource in its three forms: rain-, soil-, and vapor
water. Soils where Ilex guayusa grows have a sandy-
loam characteristic with acid pH between 4.34 and
5.01. It has low cationic capacity, high aluminum
and heavy metal content, following the pattern of
acid soils with a tendency to become poor
depending on the vegetation sustained not
including trees (32). Taking into account its light
requirements it can be considered a forest species.
It is designated as a durable heliophyte, since its
natural regeneration can be maintained at low light
levels. In fact, semi-dark sites are the most
recommended for its proliferation. Originally
guayusa seedlings need little light to meet their
functions. Moreover, in an environment free of light
exposure it tends to ramify and grow shoots, since
the terminal bud has been affected by light and
grows branches. Forming stems cast a shadow on
basal shoots that are generated on dead or little
vigorous stems, generating a “soil bed” made of
leaves and trunks, which eventually decompose and
serve as nutrients for the seedlings. No reproductive
phenophases have been reported.
Ilex guayusa is not found within the conservation
category in the vascular plant catalog, such as the red
book, proposed by the International Union for
Conservation of Nature and Natural Resources, i.e. it
is not vulnerable, it is not endangered, or in critical
danger, thus it is not a species at risk (26, 33-35). For
many years some botanists speculated Ilex guayusa
reproduced in an asexual manner, since it had lost its
flowering and fruit production capacity through years
of selection and vegetative propagation by man. This
theory was based in the lack of specimens with
reproductive organs, thus its certain taxonomic
classification (21). At present it reproduces asexually,
despite the presence of seeds. Stems without leaves
are planted for propagation (4).
In 1683 the Jesuit Juan Lorenzo Lucero reported the
Shuar natives (known as Jibaros by the Spanish
conquistadores) used Ilex guayusa in their medicinal-
magical acts in the following manner: “They placed
together these demonic herbs (Datura, Banisteriopsis
caapi, Psychotria viridis, Justicia pectoralis,
Brugmansia, Nicotiana rustica, and other
hallucinogenic plants) in addition to guayusa and
tobacco, also invented by the devil. They cooked
them in a way the little juice produced became the
quintessence, with the belief those who drank were
rewarded with the fruit of a curse by the devil for the
misfortune of many .... Lucero described the Shuar
as well disposed people, with good physical
appearance, accustomed to take several times a day
a decoction referred to as guayusa; to stay awake
for several nights without losing consciousness, when
an invasion by their enemies was feared. In the
indigenous view the guayasa ritual has a purification
significance and was used as a “bode drink. It was
consumed in high concentrations to dream, foresee
the future and guess whether the hunting or fishing
would be successful (3, 7, 19, 21, 22, 25, 36-39).
In 1756 Fray Juan de Santa Gertrudis Serra stated:
“The most beautiful leafy tree of all I have ever seen,
thick trunk, with peaceful and delightful green
leaves. The leaves have a very tasty flavor, similar to
tea, but finer and appetizing. When the beverage
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prepared with cooked leaves is drank it produces
sweating and eliminates phlegm, represses blood
ardor and eliminates heaviness, aids in digestion
with a satiety feeling, gives robustness and removes
all moodiness. When it is drank with honey
obtained from apate bees, women become
pregnant” (20, 24). Registries from 1756 indicate
high Putumayo Indians (Amaguajes and
Parayaguajes), in addition to White people have
employed Ilex guayusa leaves as a stimulant
infusion. It would be consumed in the mornings to
alleviate hunger, it was argued they did not feel
hungry from early in the morning until noon (20). In
1943 the Botanical Institute of the Central
University in Ecuador issued a bulletin informing Ilex
guayusa leaves were used by people from the
oriental region as an infusion for breakfast with the
belief this plant would “lift them up”. Moreover, it
had a fertilizing power and could be related to
getting married (20, 24, 25). In 1857 Richard Spruce
observed guayusa use among Shuar (Jibaro) natives
as an emetic to daily cleanse the stomach, as a
purgative, as a narcotic and hypnotic. Likewise, to
exonerate the body before the daily tasks, with
eschatological purification beliefs, as a ceremonial
daily mouth wash (14, 18, 22, 25, 38, 40-42).
Pleasant taste leaf infusion in the form of tea it was
used to treat all chills, venereal diseases, and for
women to become pregnant when they were sterile
many years back.
In the mid-XIX century guayusa was used for
poisoned people. In addition, burned leaves then
mixed with barley and honey was employed to treat
amenorrhea; cooked leaves to treat diarrhea and
stomach pain. Around the third quarter of the XIX
century botanists found the presence of caffeine in
leaves (38). Prehispanic Bolivian culture possibly
employed leaves as an enema (18, 43, 44).
Kallawaya’s from the Province of Bautista Saavedra
in Bolivia are known to be expert herbalists. With
over a millennium in traditional medicine practice
they are characterized for curing physical and
spiritual illnesses. One particular distinctive of this
culture is to perform brain surgery. Furthermore,
they employ over 1,000 plants, among them Ilex
guayusa a holly-like plant as an anesthetic. This use
has been described as early as 700 A.C. (45, 46).
Kallawaya are recognized by Andean people (Peru,
Bolivia, and Argentina) as “The Lords of the
Medicine Bag” (4, 47). Natives of some localities of
the Department of Nariño (Colombia) use Ilex
guayusa as a medicinal plant, in particular to
regulate menstrual cycles. The bark and wood are
used as a medicinal stimulant.
Leaf infusion against chills, as a narcotic, and
stimulant beverage. With dried leaves and branches
a beverage is prepared similar to mate from
Paraguay (Ilex paraguayensis) (29, 48).
Whole fresh plant cooked and drank with lemon and
orange serves as a diuretic, against anemia and
sorcery. Ilex guayusa cooked leaf intake with fresh
Pilea microphylla L. (preñadilla) and fresh Lycaste
gigantea Lindl. (simayuca) fruit is used for masculine
fertility. Mixed with the juice of two Citrus aurantium
L (bitter orange) serves as a vitamin supplement,
together with burned bitter orange skin is used as
incense in ceremonies. These last two preparations
are also employed against scurvy, stomach ache, high
blood pressure, as a deodorant, and for “mal de aire
(syndrome of culture filiation -bad air).
Ilex guayusa decoctions with whole Pilea microphylla
L. (preñadilla), with Eucalyptus globulus Labill
(aromatic eucalyptus) and Lycaste gigantea Lindl.
(simayuca), and sugar can be taken on a daily basis as
a diuretic, against venereal diseases, for the lungs,
and for fertility purposes (49). Oral administration of
dried Ilex guayusa leaves are used to treat blood
intoxication and diabetes (13, 50). Due to its high
caffeine content (2%) it is considered and energizing
plant (10, 14). Moreover, Ilex guayusa is employed
against drug addiction, hangover, and to eliminate
the bad taste of ayahuasca consumption (51).
In Ecuador this species is frequently used as a
refreshing and tonic beverage, with similar effects to
Asian tea or to Argentina-Paraguayan mate. It can be
purchased in most grocery stores as dried leaves. It is
claimed to have fertility properties. In addition it is
used as a stimulant, tonic, stomachic, digestive and
emetic (3, 52, 53). Aids digestion and it is stated that
cleanses the stomach and the intestines, since it has
emetic characteristics. Likewise, it has expectorant
properties, since its intake produces a warm burst
throughout the body, allowing for phlegm expulsion
from the lungs resulting from colds (10, 29, 40). In
2003 a descriptive, analytical-comparative research
was carried-out in the cities of Quito (Ecuador), Puyo
(Ecuador), and Bogota (Colombia) finding the
following uses against: sterility, diabetes, asthma, as
a diuretic, during pregnancy and lactation period, as
a mouth wash, against tiredness, muscular pain,
weight loss, as a narcotic/shaman, aphrodisiac,
purgative/emetic, and refresher. Data gathered by
traditional knowledge demonstrate a main use
(12.8%) as an emetic and stimulant. Application
techniques include baths, lavage, ointment, poultice,
intake or inhalation among others, every eight to 24
h (9). Ilex guayusa is the most used and cultured
plant by the Kichwa Indians in the Canton Loreto
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region in Ecuador. It is the most important plant in
daily life, since its consumptions every morning
brings about multiple effects such as luck for fishing
and hunting, in addition to providing protections
against snake bites (54, 55). In Peru its leaves are
employed as a dietary supplement, for prostate and
kidney protection, favoring kidney stone expulsion
(56). From the ethno-veterinary medicine point of
view Ilex guayusa is used by Shuar and Quichua
Indians in Ecuador as a psychoactive plant to
improve performance and capacities in hunting
dogs, increasing their sense of smell. This use could
be implemented by police or guard dogs to detect
explosives, illegal drugs, human remains, and other
activities of value (57).
Some studies with this plant reveal its caffeine,
triterpene, and chlorogenic content (14, 18, 58-60).
Family compound identification has been
performed through preliminary phytochemical
analysis identifying tannins and flavonoids in leaf
aqueous and ethanol extracts, respectively (61).
Polyphenol quantification evidenced 0.49 and 0.18
mg tannic acid per gram of sample for the aqueous
and ethanol extracts, respectively. Total phenol
content present in leaf methanol extract was 116.8
g of gallic acid per g of sample (62). Methanol total
extract bio-assay guided fractionation by
antioxidant and antihyperglycemic activity
identified Uvaol, by GC-MS (63). Racidi and
collaborators reported in leaf ether extract the
presence of alkaloids, steroids, terpenes and
lactonic or coumarin compounds. Moreover, in the
aqueous extracts saponins, phenols, tannins,
reducing sugars and alkaloids; and in the ethanol
extract phenols, alkaloids, reducing sugars, steroids,
terpenes, flavonoids and quinones. These authors
described Ilex guayusa phytochemical knowledge is
still very limited and other studies could suggest
new medicinal uses for this plant (19).
Other compounds present are methylxanthine, theo
bromine, theophylline, guanidine, steroids,
essential oils, isobutyric acid, nicotinic acid, ascorbic
acid, riboflavin, choline, pyridoxine, triterpenes,
chlorogenic acid and sugars among others (10, 13,
22, 64). Likewise, polyphenol content 40.1 mg/g),
L-theanine (1.3 mg/g), theobromine (0.4 mg/g), and
caffeine (32.8 mg/g) have been reported (65, 66).
In 2013 researchers from the Escuela Superior
Politécnica del Litoral, in Ecuador performed from
Ilex guayusa leaves a physicochemical,
bromatological, sensorial and microbiological study.
Phytochemical analysis revealed alkaloids,
flavonoids, reducing sugars phenols, triterpenes,
quinones, fats and oils. Bromatological study
indicated a protein content between 0.6 and 1.3%,
total fat content between 1.6 and 4.0%, total ash
content between 5.5 and 6.9%, hydrochloric acid
insoluble ash between 0.7 and 0.8%, water soluble
substances between 0.9 and 2.9%, carbohydrates
(including monosaccharides to structural
polysaccharides) between 78.4 and 83.6%.
pH value of tea prepared as an infusion oscillated
between 6.3 and 6.5, refraction index between
1.3391 and 1.3651. The infusion had a green-orange
color, with slightly fragrant aroma and indefinite
flavor. Caffeine content was 3.7%, indicating this
value depends on harvest time and ecological,
geographical and edaphic factors (10). Another study
found mean caffeine values of 2.9% for different
hydro-alcoholic extracts, where ethanol
concentration ranged between 50 and 80%, 13.8%
total solids content, pH of 4.6 and relative density of
1.01 g/mL (66).
Quantitative polyols and carbohydrate analysis of
mono- and disaccharide-type was performed using
LC-MS/MS finding values between 0.006, 0.039,
0.25, 9.8, 13.2, and 14.03 mg/g for sucrose, maltose,
sorbitol, glucose, and fructose; respectively (67). GC-
MS analysis revealed pentacyclic triterpenoid acids
such as oleanolic acid (3b-hydroxy-olean-12-en-28-
oic acid) and betulinic acid (3b-3-hydroxy-lup-20(29)-
en-28-oic acid), followed by LC-MS/MS quantification
with values of 1.18 and 18.22 mg/g; respectively
(68). Furthermore, content of 19 amino acids were
quantified by LC-MS/MS with values ranging
between 10 and 280 mg/g for glycine, asparagine,
serine, aspartic acid, glutamine, threonine, alanine,
glutamic acid, proline, lysine, valine, histidine,
methionine, arginine, tyrosine, isoleucine, leucine,
phenylalanine, and tryptophan (69).
Standardized liquid concentrate of guayusa
proximate analysis demonstrated 66.4% moisture
content, 4.9% ash, 7.0% protein, 3.5% total sugars,
0.4% total fats and 3.8% dietary fiber. Secondary
metabolite GC analysis determined the following
components: caffeine (36 mg/mL), theobromine (0.3
mg/mL), chlorogenic acids (52 mg/mL), total
polyphenols (10 mg/mL), catechin (2 mg/mL),
isoflavones (0.8 mg/mL), epicatechin (0.18 mg/mL),
epicatechin gallate (0.19 mg/mL), epigallocatechin
gallate (0.09 mg/mL), epigallocathechin (1.1 mg/mL),
kaempferol (trace), and naringin (trace) (64).
Biological activities
Studies in mice with Diabetes mellitus type I, induced
by streptomycin treatment (STZ) demonstrated oral
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administration of Ilex guayusa infusion slowed
down hyperglycemia development, reduced
glycosylated hemoglobin, polydipsia, and weight
loss (70, 71).
Sarango in 2008 reported leaf methanol extract had
an inhibitory effect against a-glucosidase with an
IC50 of 411 µg/mL (72). Other in vitro studies
evaluating Ilex guayusa leaf extracts in hexane,
ethyl acetate and ethanol demonstrated
hypoglycemic activity with inhibition of a- and b-
glucosidases, enzymes associated with diabetes
mellitus type I development. For 500 µg/mL
hexane, ethyl acetate, and ethanol extracts a-
glucosidase was inhibited at 98.4, 79.1, and 58.2%,
respectively. Likewise, for b-glucosidase inhibition
was 35.0, 52.5, and 84.2% for each extract at 1,000
µg/mL. Results suggest this plant could be
considered a possible neutraceutical in the diet of
diabetic patients (73).
Colombian medicinal plant Vademecum describes
Ilex guayusa ethanol leaf extract presents central
nervous system and sympathetic nervous system
stimulation, possibly due to caffeine high content.
Infusion consumption stimulates the cardiac
system, augments alertness, and increases the
capacity to perform physical tasks (74). Hot tea
drank at a concentration of 10 g/L three times per
day is used as a treatment for diabetes (75). In
Trujillo, Northern Peru it is traditionally used by the
medicine man as an anti-inflammatory and
antimicrobial plant. Antibacterial activity results
demonstrate leaf aqueous and ethanol extracts
have a biological activity against Staphylococcus
aureus presenting 14 mm inhibition halos (50, 76).
Methanol, ethanol and hydroalcoholic extracts at
25 mg/mL presented antifungal activity with 16 mm
halos for ethanol extract and 24 mm for the
methanol extract against Candida albicans. In
addition, a 32 mm halo was observed for the
hydroalcoholic extract against Microsporum canis
(11). Inter-institutional work carried-out by
Calderon and collaborators with 311 species,
including Ilex guayusa evaluated antiparasitic effect
against Malaria, Chagas disease and Leishmaniasis,
finding leaf ethanol extract presented an IC50 of 47,
> 50, and > 50 mg/mL against Trypanozoma cruzi,
Plasmodium falciparum, and Leishmania mexicana,
respectively (77).
Estrogen effect of Ilex guayusa leaf hydroalcoholic
extract was evaluated on ovaries, uterus, and serum
estradiol by oral administration given to albino rats
(Rattus novergicus). Used doses of 9, 18, and 36
mg/kg per day presented such effect on immature
rats. These results suggest Ilex guayusa potential
use for infertility in women (78, 79). DPPH and TEAC
assays revealed its probable antioxidant use with an
IC50 of 11.8 and 14.9 g/mL], respectively for leaf
methanol extracts (62). Furthermore oxygen radical
absorbance capacity (ORAC) in aqueous and
lipophylic media reported values of 658.9 and 0.3
µmol TE (Trolox equivalent) per gram of sample for
ORAChydro and ORAClipo, respectively (65).
Researchers of the Department of Pharmacy at
Universidad Nacional de Colombia evaluated in vitro
and in vivo antioxidant capacity in leaf aqueous and
ethanol extracts. Xanthine/xanthine oxidase
superoxide anion radical uptake measured as
inhibition of nitroblue tetrazolium (NBT) reduction
was 64 and 57% for aqueous and ethanol extracts,
respectively. Furthermore, peroxyl radical uptake
was measured in Aroxyl radical absorption capacity
ABAP/lysozyme system finding inhibition values of 15
and 18% for aqueous and ethanol extracts,
Hydroxyl radical uptake generated in the
H2O2/Fe+3/EDTA/ascorbate system demonstrated for
the aqueous extract a 42% uptake and 18% uptake
for the ethanol extract. Liver microsomal lipid
peroxidation using the non-enzymatic
Fe+2/EDTA/ascorbate/H2O2 method determined
inhibition percentage values of 93% for the aqueous
extract and 96% for the ethanol extract (61).
Ilex guayusa based cosmetic gel elaboration by
Ecuadorian researchers established a skin protective
agent in addition to having a lipolytic effect. Ilex
guayusa in vivo anti-cellulite effect was evaluated in
women between the ages of 30 and 50 years old.
Their findings evidenced a reduction in body
measurements and in the appearance of orange-
textured skin known as cellulite, proportional to the
time of treatment. This effect is likely due to caffeine
plant content (66).
Ilex guayusa could affect the nervous system if
consumed with food in great quantities (29, 52). In
the Department of Pharmacy at the Universidad
Nacional de Colombia an in vivo hepatoxicity model
was evaluated using Wistar rats. Affected animals
were induced by CCl4 administration, and as a
positive control Sylimarin was used (61).
Histopathological study did not reveal any
considerable toxicity signs (80). Colombian medicinal
plant Vademecum indicated Ilex guayusa infusion or
decoction beverage consumption did not present
signs of acute toxicity (74). Multidimensional tests
using de 1,000, 500, 250, and 125 mg/kg ethanol
extract did no cause lethality in animals. In addition,
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repeated infusion doses were safe (74, 80). Due to
its high caffeine content it is not recommended for
pregnant women. Excess consumption can produce
vomit and alterations in the CNS (74).
In a collaborative work between the USA and Perù,
researchers evaluated alcohol and water extract
toxicity of 341 plants, including Ilex guayusa using
the brine shrimp lethality test. Results
demonstrated a LC50 > 10,000 µg/mL for the
aqueous extract and 300 µg/mL for the ethanol
extract (81). Achuar indians from the Ecuadorian
Amazon pointed out Ilex guayusa mix with other
plants can be toxic. For example, its decoction with
Psidium guajava produces a poisonous beverage
(12). Kapp and collaborators evaluated standardized
liquid concentrate of guayusa using in vitro
genotoxicity tests, with Bacterial reverse mutation
test (Ames test). Furthermore, a study of
chromosome aberrations in human lymphocytes
was performed. Ames test established a negative
result. Likewise, no structural or numeric
aberrations were observed for the chromosome
study. Acute toxicity by oral administration with a
5,000 mg/kg dose in female rats established a
salivation response, hypoactivity, abnormal
breathing, stooped posture, decreased and soft
feces. All animals recuperated at the third day of
administration and continued healthy until day 14
of the study. Necropsy did not evidence any severe
abnormalities. Results suggested oral median lethal
dose in female rats was > 5,000 mg/kg. 90 day
subchronic toxicity test by oral administration at
1,200, 2,500, and 5,000 mg/kg in female and male
rats revealed no toxicity associated with
standardized liquid concentrate of guayusa.
In females a high neutrophil and basophil count was
found, depending on administered Ilex guayusa
dose. Additionally, a distinct adaptive hypertrophy
in salivary glands was observed, with a greater
impact on females, depending on dose. Moreover,
blood chemistry was altered with the following
values increasing in blood serum: aspartate
aminotransferase, serum alanine aminotransferase,
and cholesterol. A body weight reduction and food
efficiency, decreased triglycerides values, and
diminished fat pad weight were observed.
However, no noxious effects were observed.
Therefore, this study indicated no harmful effect in
liquid concentrate of guayusa on this model system
The authors thank COLCIENCIAS (Administrative
Department of Science, Technology and Innovation)
for by support the Doctoral Thesis of LGSC. In
addition authors manifest their gratitude to
Engineers Luis Enrique Acero Duarte and Leonor
Rodriguez for their support and supplying Ilex
guayusa photographs (Mogambo Sendero
Ambiental). This work was funded by the Academic
Vice-Rectory and Vice-Rectory for Research of the
Pontificia Universidad Javeriana for Project 5392.
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Figure 1. Flowers, leaves and steams of Ilex guayusa (27).
... Thus, allowing for ethnobotany and medicinal plant studies in search of similar properties in native and endemic plants (Bussmann et al., 2018). Our previous studies describe several plant species in Colombia, including Ilex guayusa and Piper marginatum used by indigenous and rural communities for the treatment of oral cavity diseases (Bernal et al., 2011;García-Barriga, 1992; Rojas et al., 2006;Sequeda-Castañeda, 2021;Sequeda-Castañeda et al., 2015;Sequeda-Castañeda et al., 2016). P. marginatum Jacq. is present in the neotropic from Guatemala to Brazil and the Caribbean. ...
... Moreover, I. guayusa is present in the Western Amazon (Peru, Colombia, and Ecuador). It has a long ethnobotanical data, where it has been used as a mouthwash, herbal tea and energizing drink, among others (Lema-Paguay et al., 2017;Sequeda-Castañeda et al., 2016). Phytochemically it contains caffeine, triterpenes, chlorogenic acids, tannins, and flavonoids (Arteaga-Crespo et al., 2020;Chianese et al., 2019;Dueñas et al., 2016;García-Barriga, 1992;Gupta et al., 2008;Lema-Paguay et al., 2017;Radice & Vidari, 2007;Sequeda-Castañeda, 2021;Sequeda-Castañeda et al., 2016;Wise & Negrin, 2020 ;Wise & Santander, 2018). ...
... It has a long ethnobotanical data, where it has been used as a mouthwash, herbal tea and energizing drink, among others (Lema-Paguay et al., 2017;Sequeda-Castañeda et al., 2016). Phytochemically it contains caffeine, triterpenes, chlorogenic acids, tannins, and flavonoids (Arteaga-Crespo et al., 2020;Chianese et al., 2019;Dueñas et al., 2016;García-Barriga, 1992;Gupta et al., 2008;Lema-Paguay et al., 2017;Radice & Vidari, 2007;Sequeda-Castañeda, 2021;Sequeda-Castañeda et al., 2016;Wise & Negrin, 2020 ;Wise & Santander, 2018). Moreover, Racidi and Vidari (2007) reported presence of alkaloids, steroids, terpenes, and lactonic compounds (Radice & Vidari, 2007). ...
Full-text available
Objective To determine if native Colombian Piper marginatum Jacq. and Ilex guayusa Loes plant extracts have a remineralizing effect on teeth with Amelogenesis imperfecta in comparison with the commercial products Clinpro-3M and Recaldent™. Material and Methods An in vitro study was carried out with 128 human teeth slices (64 healthy and 64 with Amelogenesis imperfecta) on which an initial Raman spectroscopy was performed followed by Raman spectroscopies at 0, 24, 48, and 72 h to determine possible remineralization by observing mineral increase or decrease as a result of P. marginatum Jacq. and I. guayusa Loes extract application in comparison to control substance (Clinpro and Recaldent™) application. Obtained data were analyzed using a bivariate method with a t unidirectional test. Significant differences among groups were determined by an ANOVA with Dunnett post hoc tests. Results Native I. guayusa Loes and P. marginatum Jacq. Colombian plants extracts exhibited phosphate and orthophosphate mineral apposition, where P. marginatum Jacq. presented better results. Conclusions Native Colombian I. guayusa Loes and P. marginatum Jacq plant extract might in the future be useful for dental tissue remineralization, as they induced phosphate and orthophosphate mineral apposition, main components of tooth enamel. These types of natural compounds can become an alternative to fluorine, whose ingestion is harmful to the human body.
... Ilex guayusa Loes. (Aquifoliaceae) is a shrub or a small tree [1] native to the Neotropics [2,3], distributed along the Amazon region of Colombia, Ecuador, Peru, and Bolivia [1][2][3][4][5][6]. It is commonly used to prepare tea-like infusions similar to other Amazonian beverages such as yerba mate (I. ...
... Ilex guayusa Loes. (Aquifoliaceae) is a shrub or a small tree [1] native to the Neotropics [2,3], distributed along the Amazon region of Colombia, Ecuador, Peru, and Bolivia [1][2][3][4][5][6]. It is commonly used to prepare tea-like infusions similar to other Amazonian beverages such as yerba mate (I. ...
... [6,9], and Ecuador appears to be the main center for its cultivation, especially towards the eastern Andean slopes and the adjacent Amazonian piedmont [4,[9][10][11]. Growing as part of secondary forests, guayusa proliferates on sandy-loam soils with acidic pH, and in humid and semi-dark environments [2]. Extensive use of this species by humans throughout its range of distribution strongly suggests some degree of domestication [3]. ...
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Ilex guayusa Loes. is a shrub native to the Neotropics, traditionally consumed as an infusion. Despite its cultural value and extensive use, genetic research remains scarce. This study examined the genetic and clonal diversity of guayusa in three different Ecuadorian Amazon regions using 17 species-specific SSR markers. The results obtained suggest a moderately low degree of genetic diversity (He = 0.396). Among the 88 samples studied, 71 unique multilocus genotypes (MLGs) were identified, demonstrating a high genotypic diversity. A Discriminant Analysis of Principal Components (DAPC) revealed the existence of two genetic clusters. We propose that a model of isolation-by-environment (IBE) could explain the genetic differentiation between these clusters, with the main variables shaping the population’s genetic structure being temperature seasonality (SD × 100) (Bio 4) and isothermality ×100 (Bio 3). Nonetheless, we cannot dismiss the possibility that human activities could also impact the genetic diversity and distribution of this species. This study gives a first glance at the genetic diversity of I. guayusa in the Ecuadorian Amazon. It could assist in developing successful conservation and breeding programs, which could promote the economic growth of local communities and reinforce the value of ancestral knowledge.
... Additionally, native people used to drink guayusa tea during an early morning ceremony that promotes body purification by drinking a large amount of beverage and then vomiting. During the ritual, people would talk about the dreams and analyse them to plan the activities of the day, based on the meanings that those dreams had revealed (Sequeda-Castañeda et al., 2016). ...
... Results were found to be lower than the data reported by Pardau et al. (2017) (5.44 g gallic acid equivalents/100 g d.w.). It has been shown that there is an high correlation between TPC and antioxidant activity (Sequeda-Castañeda et al., 2016). ...
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The present study carried out the optimisation of the total polyphenol content (TPC) extraction assisted by ul-trasound in Ilex guayusa leaves applying response surface methodology (RSM). Also, the evaluation of the anti-oxidant activity of the extract obtained under the optimal extraction conditions was performed. The effect of the variables like, time of sonication, temperature, ethanol/water ratio and solid/liquid relationship and the interactions between them were analysed through the use of a factorial design 2^4. The significant factors were considered for the optimisation, employing a Box-Behnken Design, and the TPC as response variables. It was found that a quadratic model was adequate, with an adjusted R 2 value of 0.9367. The optimal conditions proposed , by the response surface model were: an extraction temperature of 60 C, sonication time of 29.9 min and ethanol/water ratio of 76.8/23.2. The optimised leaves extract of I. guayusa show a TPC of 3.46 (AE0.17) g gallic acid equivalents/100 g d.w. Radical scavenger activity of the obtained extract at optimum conditions, was performed through the FRAP and ABTS methods, given as result: 0.080 mmol TROLOX equivalents/100 g d.w. and 40.71 μmol TROLOX equivalents/g d.w., respectively. Due to the present findings, I. guayusa extracts can be proposed as a promising component for functional beverages, cosmetic and pharmaceutical formulation.
... Guayusa concentrate was found to be negative in in-vitro genotoxicity tests in human lymphocytes, and was also found to have an LD 50 of >5,000 mg/kg in female rats [17]. Further toxicology studies on rats have reported negative findings [18]. In brine shrimp, the LC 50 for an aqueous extract of guayusa was assessed to be greater than 10,000 µg/mL [19]. ...
... This usage began in antiquity and continues across a large modern consumer market in Ecuador today. This conclusion is consistent with previous negative findings of in-vitro [17], animal [18,19] and human [20] toxicology studies of guayusa extract exposure. We hold the view that with further chemical compositional analysis of guayusa, a strong case could be mounted for the acceptance of dried guayusa leaves, under new European Union novel food legislation covering traditional foods. ...
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Guayusa (Ilex guayusa Loes.) is a herbal tea that has been consumed for centuries as a traditional food in western Amazon regions where it is now valued as an antioxidant and a stimulant agent. Currently there is intense commercial activity to expand the consumption of guayusa as a healthy energy drink in Europe, where it is classified by law as a novel food. European Union novel food legislation permits traditional foods to be placed on the market if a history of safe use in a non-EU country can be established. However, a scientific assessment of the 'safe' use of guayusa is lacking. This study investigates the safety of guayusa consumption, analysing provincial hospital admissions data; national disease register data; national toxicology agency call centre data; and national food safety authority data. In doing so we present a crucial analysis for the novel food premarket risk assessment of dried guayusa leaves, based on the well-established use of this traditional food in Ecuador. Within a three-year period there was one minor adverse effect reported nationally, related to the stimulant properties of guayusa. However, there were no hospital presentations, no product safety notifications and no disease register records of guayusa-related illness. Comprehensive records of unrelated food safety risks demonstrate that Ecuador's surveillance and reporting system has sufficient rigor to identify risks should they exist. We conclude that there is a history of safe use of guayusa in Ecuador. Establishing safe guayusa consumption is an important milestone for the authorization of dried guayusa leaves as a novel food with claimed health benefits in Europe. Consequently, this study has significance for international product development of guayusa as an antioxidant energy drink.
... Nowadays, guayusa leaves have gained commercial value, and they have exported worldwide to be consumed as an herbal tea due to indigenous knowledge and chemical properties (Wise and Negrin 2020). Scientific findings have demonstrated the antioxidant, antiglycemic, antifungal, antibacterial, and anti-inflammatory effects of guayusa leaves extracts (Sequeda-Castañeda et al. 2016). Other studies have reported the concentration of caffeine, theobromine and theophylline in leaf alcoholic extracts, being caffeine the major alkaloid quantified (Negrin et al. 2019). ...
The use of guayusa (Ilex guayusa Loes.) leaves as functional food has increase recently. This work discusses the antioxidant activity and volatile compounds of guayusa leaves extract and fractions. The methanol crude extract was obtained by maceration, subsequently hexane, chloroform, ethyl acetate, and aqueous fractions were collected by solvent-solvent partition. Total phenolic content (TPC), total flavonol/flavone content (TFC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, and ferric reducing antioxidant power (FRAP) were measured by ultraviolet-visible (UV-Vis) spectrophotometry. The results revealed that ethyl acetate fraction showed highest inhibition against DPPH radical (93.86 ± 0.95%) at 500 µg/mL, and reduce the ferric-tripyridyltriazine complex (Fe3+-TPTZ) at 1619.81 mg trolox equivalent (TE)/g, followed by aqueous fraction. This bioactivity could be related to phenolic acids, flavones and flavonols content, as well as the caffeine, dodecanoic acid isopropyl ester, caffeic acid, and malic acid identified by gas chromatography-mass spectrometry (GC-MS). These findings support the antioxidant properties of this plant material.
... 62 Further toxicology studies on rats have reported negative findings. (14) In brine shrimp, the LC50 for an aqueous extract of guayusa was assessed to be greater than 10,000 µg/mL. 61 ...
Technical Report
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Dried leaves of guayusa (Ilex guayusa Loes.) have a continuing history of safe use (including the last 25 years) as a herbal tea outside of the European Union. This continuing safe use has been demonstrated since antiquity in the western Amazon, foremost in Ecuador. Furthermore, chemical composition analyses demonstrate that guayusa presents no greater risk to human health than do tea or other herbal teas currently accepted for sale in EU markets (e.g. Camellia sinensis green tea or black tea or other herbal teas such as yerba mate). Guayusa is currently enjoying a rapid increase in its popularity in international consumer markets, with 100 tons in 2017 being exported, principally to the USA. As a rapidly growing international commodity, with a known chemical composition and a history of safe use, we submit this notification for placement of dried leaves of guayusa in EU markets as a traditional food from a third country.
... In Colombia, it is known as "toothhealer" or "small cord" [27,28,36]. On the other hand, Ilex guayusa Loes, known as "guayusa," is a native plant of the neotropics with natural distribution in Colombia, Ecuador, Peru, Bolivia, and Brazil [29,37]. In previous studies, different extracts with antimicrobial activity derived from species of the Piper genus and Ilex guayusa Loes have been evaluated [38][39][40]. ...
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Background: Chronic periodontitis is a multifactorial infectious disease, where multiple bacteria, such as Porphyromonas gingivalis, Prevotella intermedia, and Fusobacterium nucleatum are implicated. The main purpose of researching natural products is to find substances or compounds with antimicrobial activity. Aim: The objective of this work was to determine antimicrobial activity from extracts and obtained fractions from Piper marginatum Jacq and Ilex guayusa Loes on P. gingivalis ATCC 33277, F. nucleatum ATCC 25586, and P. intermedia ATCC 25611. Methods: Total ethanol extracts were obtained from both plants. Fractions were obtained from total ethanol extracts with amberlite as a stationary phase employing hexane, acetone, and ethanol-water as solvents. Qualitative and quantitative phytochemical characterization was performed on total ethanol extracts from both plants. Antimicrobial activity from total ethanol extracts and fractions from both plants were evaluated on P. gingivalis ATCC 33277, F. nucleatum ATCC 25586, and P. intermedia ATCC by the well diffusion method with Wilkins-Chalgren agar. Results: Piper marginatum Jacq total ethanol extract presented antimicrobial activity against all three bacteria, whereas Ilex guayusa Loes was only efficient against P. gingivalis ATCC 33277 and P. intermedia ATCC 25611, with inhibition halos from 9.3 to 30 mm. Ilex guayusa Loes obtained fractions presented antimicrobial activity against all three microorganisms evaluated, with inhibition halos ranging from 9.7 to 18.7 mm. In regards to Piper marginatum Jacq fractions, inhibition halos were between 8.3 and 19 mm, against all three microorganisms evaluated; only hexane fraction did not present antimicrobial activity against F. nucleatum ATCC 25586. Conclusion: Piper marginatum Jacq and Ilex guayusa Loes total ethanol extracts and fractions presented outstanding antimicrobial activity against P. gingivalis ATCC 33277, P. intermedia ATCC 25611, and F. nucleatum ATCC 25586.
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Se valida la hipótesis que el uso tradicional de plantas introducidas y nativas es análogo en Ecuador. Ciento veinticuatro entrevistas se desarrollan en 13 provincias de Ecuador, a 99 mujeres y 25 hombres, 107 del total son mestizos y 17 son indígenas, mayoritariamente comerciantes con estudios primarios y constan de 1 a 60 años de experiencia, que adquirieron el conocimiento del uso tradicional de las plantas de sus padres o madres principalmente. Se registra el uso de 274 especies, 138 (50,36%) del total son introducidas y 136 (49,63%) son nativas, 3 de las cuales son endémicas (1,09%); pertenecientes a 224 géneros incluidos en 88 familias botánicas, originarias de América (61,85%), Asia (15,68%), Europa (10,45%), África (9,58%) y Oceanía (2,44%). Se presentan 28 usos generales, particularmente como medicinal (71 usos terapéuticos), destacando el uso como antiinflamatorio, analgésico, antibiótico, antiespasmódico, diurético, sedante y antigripal. El conocimiento tradicional de las plantas no varía significativamente entre etnias y género; lo opuesto ocurre a nivel de edad y entre especies nativas e introducidas. Existe gran concordancia entre los informantes sobre los usos etnomedicinales de las plantas con un valor del Factor de Consenso de los Informantes (FIC) de 0,98.
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Antecedentes: La diversidad botánica del Perú ha hecho que actualmente se esté promoviendo el uso de plantas medicinales en las instituciones de salud a nivel nacional. El objetivo de esta revisión es proporcionar información científica que respalde las propiedades terapéuticas del agracejo como Berberis vulgaris. Métodos: Se buscó información en bases de datos científicas, Plantlist, Scopus, PubMed, Google Académico y repositorios de tesis universitarias sobre, información etnobotánica y etnofarmacológica y se comparó con el Berberis rigida Hieron, Berberis vulgaris, Ilex guayusa Loes, Vellesia glabra (Cav), dado que todas ellas presentan también el nombre común de agracejo. Resultados: Actualmente existen 3 familia y 4 géneros que presentan a una especie conocida como agracejo, se destaca 5 tesis universitarias peruanas en los últimos 10 años que abordan las utilidades del género Berberidaceae y sus especies, teniendo como mayor utilidad la tintura. La mayor información científica, 1 363 estudios entre etnobotánico y etnofarmacológicos, respaldan la utilidad del B. vulgaris, aunque son provenientes de países orientales y solo 3 son peruanas. Conclusiones: La información que respalda la utilidad del B. vulgaris (agracejo), proviene de publicaciones internacionales (Irán, Pakistán), además no se cuenta con información científica etnobotánica y etnofarmacológica peruana, esto genera un riesgo de confusión de especies, más aún si presenta el mismo nombre común, y características fitoterapéuticas similares. Estas dos situaciones originarían que se adopte información de otros países, lo que a su vez pone en riesgo de posible inefectividad del tratamiento con medicina tradicional. Palabras claves: Berberis, indicación fitoterapéutica, medicina tradicional, plantas medicinales, agracejo, metabolitos secundarios, berberidaceae, aquifoliaceae, apocynaceae.
In general, preparations of coffee, teas, and cocoa containing high levels of polyphenols, L-theanine and other bioactive compounds selectively enhance mood and cognition effects of caffeine. This review summarizes the bioactive components of commonly consumed natural caffeine sources (e.g. guayusa, mate and camellia teas, coffee and cocoa) and analyzes the psychopharmacology of constituent phytochemicals: methylxanthines, polyphenols, and L-theanine. Acute and chronic synergistic effects of these compounds on mood and cognition are compared and discussed. Specific sets of constituent compounds such as polyphenols, theobromine and L-theanine appear to enhance mood and cognition effects of caffeine and alleviate negative psychophysiological effects of caffeine. However, more research is needed to identify optimal combinations and ratios of caffeine and phytochemicals for enhancement of cognitive performance.
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La protección del patrimonio natural del Ecuador representa un reto para todos, especialmente si se considera que la desaparición de las especies endémicas o restringidas exclusivamente al Ecuador implica su extinción global. La información básica acerca de las especies, los ecosistemas y su estado de conservación sigue siendo insuficiente para cuantificar con precisión la magnitud de las amenazas a la conservación de las especies. A nivel mundial, apenas se ha evaluado el estado de conservación de 4% de las especies vegetales (Baillie et al. 2004) un porcentaje bajo si se considera que las predicciones de uso y abuso de las plantas en el futuro inmediato crecerán proporcionalmente con la población humana. Según Schatz (2009) a fines de siglo la población humana llegará a los nueve o diez billones de habitantes y necesitará duplicar la producción de alimentos e incrementar espacios para vivienda y usar más plantas silvestres para alimento, combustible, construcción y medicina. Por eso, resulta urgente disponer de información científica sistematizada para tomar decisiones responsables sobre el manejo y protección de los ambientes naturales. El Libro Rojo de las Plantas Endémicas del Ecuador busca contribuir a este propósito. Según la Unión Internacional para la Conservación de la Naturaleza (UICN) hasta el año 2000 solamente se habían asignado categorías de amenaza a 461 especies de plantas endémicas del Ecuador. En el año 2000 se publicó la primera edición del “Libro Rojo de las Plantas Endémicas del Ecuador” (Valencia et al. 2000), en el cual se analizó el estado de conservación de 4011 especies endémicas del país. En esta segunda edición, en base a información actualizada sobre la distribución y la taxonomía de las especies, se evalúa el estado de conservación de 4500 especies de plantas endémicas y se asigna una categoría de amenaza a cada una de ellas según los mismos parámetros usados en Valencia et al. (2000) (UICN Categorías y criterios utilizados para la Lista Roja versión 3.1 disponible en: categories-and-criteria/2001-categories-criteria.) La presente edición de este libro es un trabajo de colaboración entre botánicos e instituciones donde se guardan colecciones de plantas ecuatorianas. El Herbario QCA de la Pontificia Universidad Católica del Ecuador, lideró este proyecto en el cual participaron las siguientes instituciones: Herbario de Loja (LOJA), Herbario Nacional del Museo Ecuatoriano de Ciencias Naturales (QCNE), Herbario del Padre Luis Sodiro S.J. (QPLS), así como los herbarios del Missouri Botanical Garden (MO), Aarhus University (AAU), Universidad de Göttingen (GOET) y muchas otras donde residen nuestros más de 80 colaboradores. En la presente edición, al inicio de cada familia, se describen los cambios observados en estos últimos diez años. En los capítulos introductorios también se incluyen perspectivas generales de conservación de los sistemas terrestres del Ecuador y de cada región natural del país.
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p>De los 17 países megadiversos del mundo cuatro de ellos se ubican en la zona andina y concentran el 75% de la diversidad en especies de animales y plantas, estos son: Colombia, Perú, Venezuela y Ecuador (Estrella et al. 2005). La Amazonía ecuatoriana representa una de las áreas con mayor biodiversidad del planeta y por su enorme variedad de plantas se convierte en una fuente de investigación de interés permanente, especialmente para el desarrollo de nuevas materias primas del mercado farmacéutico, cosmético y alimentario. Al interés comercial se unen también el científico y el antropológico, sobre todo cuando se trata de recuperar una de las plantas sagradas de las nacionalidades indígenas que habitan en la región amazónica ecuatoriana conocida con el nombre vernáculo de guayusa (Ilex guayusa Loes.) y que es usada tradicionalmente por los Achuar y mestizos en forma de infusión. Dentro de este contexto, es fundamental aclarar que el conocimiento fitoquímico de la guayusa es limitado y la literatura científica es escasa, razón por la cual es necesaria una profunda investigación científica con el fin de evaluar su actividad biológica o farmacéutica y los posibles usos comerciales. Actualmente, los pocos datos fitoquímicos de esta planta solo revelan datos de su contenido en cafeína, así como la presencia de triterpenos y ácidos clorogénicos (Rosero Gordón 2006-2007); por lo tanto, aún no se pueden explicar todas las propiedades curativas que la tradición popular le atribuye. Así, los objetivos de esta investigación fueron: 1. Desarrollar un fitofármaco con base en Ilex guayusa Loes. que actúe como un coadyuvante en el tratamiento de manifestaciones sintomáticas como gripe, jaqueca y fiebre. 2. Identificar las familias químicas presentes en Ilex guayusa Loes. 3. Desarrollar la preparación de un extracto adecuado a la formulación final. 4. Desarrollar un método de cromatografía líquida de alta resolución (CLAR) para la dosificación de la cafeína en el producto final. 5. Establecer los parámetros de calidad del producto transformado. 6. Realizar un proceso productivo apto a las condiciones tecnológicas locales. El enfoque de la investigación se puede resumir en nueve etapas principales (Figura 1), las cuales deben ser completadas con el desarrollo de un prototipo sometido a estudios preclínicos y clínicos, pruebas industriales y finalmente, el producto desarrollado debe tener un plan de comercialización.</p
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Bacterial infections and inflammation are among the ailments treated by traditional healers. The World HealthOrganization has expressed high interest in traditional medicine, and it is important to demonstrate scientifically thatremedies employed in folk medicine are indeed therapeutically active. In this communication we report on antibacterial assaysfor 171 plant species, conducted under simple laboratory conditions in a private clinic in Trujillo, Peru. The aim of the studywas to scientifically test if plants used in traditional medicine for the treatment of infections showed indeed antibacterialactivity. Extracts of samples of 171 species were screened for antibacterial activity against Staphylococcus aureus and Escherichiacoli, using agar-diffusion method. 14 species tested as traditional water extracts and 78 species extracted in ethanol showedactivity against al least one of the bacteria. Simple laboratory conditions can be applied to validate the antibacterial propertiesof plants used in traditional medicine. While folk-medicinal uses can provide clear leads for scientific trials, many plantstraditionally used against infections did not show any antibacterial activity, while plants used for different purposes yieldedsubstantial activity. To make the most of these leads plant uses have to be very carefully documented however. What has tobe taken into account is, that most traditional remedies are prepared as cocktails of different plants, where plant compoundspossibly enhance and complement each other, and bioassays need to be extended to cover such compound preparations Antibacterial activity of northern-peruvian medicinal plants. Available from: [accessed Dec 21, 2015].
Type-1 diabetes mellitus (IDDM) is a chronic degenerative disease with complications which can be devastating. There is an increasing body of research suggesting that prevention of IDDM by the avoidance of cow's milk and by the supplementation of niacinamide may be possible. This article will explore this research. The course the disease takes also may not be inevitable. Modification of diet and lifestyle factors as well as a comprehensive program of nutritional and botanical supplementation may help prevent the complications often encountered, such as neuropathy, retinopathy, nephropathy, micro- and macroangiopathy, and cataracts. This article will review the research on specific nutrients, botanicals, dietary and lifestyle factors, and their application in type-l diabetes. References.
Tea from the leaves of guayusa (Ilex guayusa) has a long history of consumption by Ecuadorian natives in regions where the plant is indigenous. The tea contains the methylxanthines caffeine and theobromine as well as chlorogenic acids, flavonoids, and sugars. Various studies were performed to evaluate the general and genetic toxicology of a standardized liquid concentrate of guayusa (GC). Guayusa concentrate was found to be negative in in vitro genotoxicity tests including the Ames test and a chromosome aberration study in human lymphocytes. The oral median lethal dose (LD50) of GC was >5,000 mg/kg for female rats. Guayusa concentrate was administered to male and female rats in a 90-day subchronic study at 1,200, 2,500, and 5,000 mg/kg/d of GC and a caffeine-positive control at 150 mg/kg/d corresponding to the amount of caffeine in the high-dose GC group. Effects observed in the GC-treated groups were comparable to those in the caffeine control group and included reductions in body weights, food efficiency, triglycerides values, and fat pad weights and increases in blood chemistry values for serum aspartate aminotransferase, serum alanine aminotransferase, and cholesterol and adaptive salivary gland hypertrophy. No signs of incremental toxicity due to any other components of guayusa were observed. The studies indicate no harmful effects of GC in these test systems.