ArticlePublisher preview available

Immunomodulatory Properties of Filamentous Fungi Cultivated through Solid-State Fermentation on Rapeseed Meal

July 2017
Applied Biochemistry and Biotechnology 182(3)

DOI:10.1007/s12010-016-2370-7

Authors:

Anne Bourdillon

Avril

Joelle Deconinck

UMR PAM Procédés Alimentaires et Microbiologiques

Water extracts from solid-state fermentation (SSF) on rapeseed meal using filamentous fungi exhibit interesting immunomodulatory activities in vitro. Immunomodulation was determined by the capacity of the compounds to activate blood neutrophils and to influence cytokine production in human peripheral blood mononuclear cells (PBMC) and mouse bone marrow-derived macrophages (BMDM). Among the strains tested, Aspergillus sojae mycelium and SSF extracts were the most promising in terms of enhancing the immune response. The filamentous fungus was also successfully cultivated in a pre-pilot bioreactor with forced aeration. The results indicated that the extracts not only activated blood neutrophils but also significantly modulated IL-1β cytokine levels with lipopolysaccharide (LPS)-stimulated PBMC and BMDM without any cytotoxicity in immune cells. IL-1β was down-regulated in a dose-dependent manner in the presence of A. sojae crude mycelium and SSF extract with PBMC, which indicated that there was an anti-inflammatory activity, whereas IL-1β secretion was up-regulated in the presence of stimulated BMDM with the highest concentration that was tested (100 μg/mL). The non-fermented rapeseed had no effect at the same concentration. SSF culture, as a natural product, may be a good source for the development of functional feed with an immunostimulating effect or could potentially be used in medicinal applications.

CO2 production (respirometric profile) and substrate dry matter weight loss of the different filamentous fungi, including Aspergillus oryzae (■), Aspergillus sojae (○), Rhizopus oryzae (▲), and Rhizopus oligosporus (□) grown on rapeseed meal at 25 °C. Control flasks contained rapeseed meal incubated with sterile water but not inoculated (x). There was no dry matter weight loss for the control flask

…

Percentage of activated neutrophils in blood alone (unstimulated), blood with the bacterial extract (stimulated, control kit), blood with rapeseed meal alone, and blood with a 3-day-old SSF culture of rapeseed meal with Aspergillus oryzae, Aspergillus sojae, Rhizopus oryzae, and Rhizopus oligosporus

…

Percentage of cell viability and concentration of IL-1β secreted in the cell medium by PBMC in the culture medium alone, the culture medium supplemented with 10 ng/mL LPS, the culture medium supplemented with 10 ng/mL LPS plus the two anti-inflammatory internal references (ZVAD, PRED) and the culture medium supplemented with 10 ng/mL LPS and Aspergillus sojae mycelium or SSF culture extract tested at four concentrations (0.125, 0.25, 0.5, and 1 mg/mL). ***Significant difference determined by variance analysis (ANOVA)

…

Percentage of cell viability and concentration of IL-1β secreted in the cell medium by BMDM in the culture medium alone, the culture medium supplemented with 1 μg/mL LPS, the culture medium supplemented with 1 μg/mL LPS plus the two anti-inflammatory internal references (ZVAD, PRED), and the culture medium supplemented with 1 μg/mL LPS and rapeseed meal or Aspergillus sojae SSF culture extract tested at three concentrations (1, 10, and 10 μg/mL). ***Significant difference determined by variance analysis (ANOVA)

…

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Immunomodulatory Properties of Filamentous Fungi

Cultivated through Solid-State Fermentation

on Rapeseed Meal

Stéphanie Sutter

1,2

&France Thevenieau

Anne Bourdillon

&Joëlle De Coninck

1,2

Received: 3 October 2016 /Accepted: 12 December 2016 /

Published online: 16 December 2016

#Springer Science+Business Media New York 2016

Abstract Water extracts from solid-state fermentation (SSF) on rapeseed meal using filamen-

tous fungi exhibit interesting immunomodulatory activities in vitro. Immunomodulation was

determined by the capacity of the compounds to activate blood neutrophils and to influence

cytokine production in human peripheral blood mononuclear cells (PBMC) and mouse bone

marrow-derived macrophages (BMDM). Among the strains tested, Aspergillus sojae myceli-

um and SSF extracts were the most promising in terms of enhancing the immune response.

The filamentous fungus was also successfully cultivated in a pre-pilot bioreactor with forced

aeration. The results indicated that the extracts not only activated blood neutrophils but also

significantly modulated IL-1βcytokine levels with lipopolysaccharide (LPS)-stimulated

PBMC and BMDM without any cytotoxicity in immune cells. IL-1βwas down-regulated in

a dose-dependent manner in the presence of A. sojae crude mycelium and SSF extract with

PBMC, which indicated that there was an anti-inflammatory activity, whereas IL-1βsecretion

was up-regulated in the presence of stimulated BMDM with the highest concentration that was

tested (100 μg/mL). The non-fermented rapeseed had no effect at the same concentration. SSF

culture, as a natural product, may be a good source for the development of functional feed with

an immunostimulating effect or could potentially be used in medicinal applications.

Keywords Solid-state fermentation .Rapeseed meal .Filamentous fungi .β-glucan .

Immunomodulation .Immune cells

Appl Biochem Biotechnol (2017) 182:910–924

DOI 10.1007/s12010-016-2370-7

*Stéphanie Sutter

stephanie.sutter@welience.com

Welience, Plateforme de Prédéveloppement en Biotechnologies, site INRA, 17 rue de Sully,

21000 Dijon, France

University Bourgogne Franche-Comté, AgroSup Dijon, PAM UMR A 02.102, F-21000 Dijon,

France

Groupe Avril-Direction Innovation, 11 Rue de Monceau, 75378 Paris, Cedex 08, France

Content courtesy of Springer Nature, terms of use apply. Rights reserved.

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