Long–eared Owls (Asio otus) gathers during winter months in typical communal roosts often on traditional sites in or near human settlements and such behaviour has been registered in Serbia and worldwide. This paper presents the results of research on Long-eared Owls micro habitat and tree selection in Serbia during winter period. The survey was done during winter 2006/2007 on 139 roosting sites, using census method. The results show that the settlements are the most important habitats during the winter period, holding 94,96% of registered roosts, and 96,83% of counted owls. The most important micro habitats were public building yards with 28,08% of roosts and 28% of counted owls. A total of 78,41% of roosts and 84,57% of specimens were registered on public green fields (parks, tree lines, public building yards and combination of these types). A total of 83% of owls were roosting on coniferous trees. The most frequent genera among the trees were: Thuja, Picea, Pinus, Abies, Betula and Cupressus. Preserving the presence and the diversity of green fields in settlements is crucial for Long-eared Owl conservation.
