Chemotaxis Studies Of Pseudomonas putida KT 2440 And Mutants In Chemoreceptors For Efficient Colonization Of Tomato Plants

Abstract

130 Abstract: An alternative for increasing the yield of crops is the use of PGPR, for this purpose the colonization is a critical process. In this work the chemotaxis toward tomato plants and bacteria colonization were evaluated for P. putida KT2440 and mutant strains in specific chemoreceptors for GABA and succinato. Axenic seedlings of three days were placed at one end of the recipients with soft agar (3g / L) and the opposite end vertically inoculated 100 µl of the explored bacterial strain and the number of CFU/ml for 1-6 days after sowing was recorded. At 3 days post-inoculation mcpG mutant showed less efficient colonization than wild type and mcpS mutant. At 4 days post inoculation two mutant strains were affected in colonization, while 6 days only mcpG-strain was affected. When the wild type and mutant strains mcpG-were coinoculated in chemotaxis systems, again was observed decreased colonization ability for the mutant strain. The mutation in the McpG and McpS chemoreceptors affect the colonization process. Chemotaxis is vital for efficient rhizosphere colonization which could have implications for growth promotion purposes.

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JCBPS; Special Issue, Section B; 30 Nov. 2014 Vol. 4, No. 5, 130. E- ISSN: 2249 –1929 7
Journal of Chemical, Biological and Physical Sciences
An International Peer Review E-3 Journal of Sciences
Available online atwww.jcbsc.org
Section B: Environmental Biotechnology
CODEN (USA): JCBPAT Abstract
130 JCBPS; Section B: Environmental Biotechnology ; Special Issue; 30 Nov.2014, Vol. 4, No. 5, 130
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Chemotaxis Studies Of Pseudomonas putida KT 2440 And
Mutants In Chemoreceptors For Efficient Colonization Of
Tomato Plants
Dalia Juárez-Hernández1, Tino Krell2, Andrés Corral-Lugo2, A. Laura
Hernández-Tenorio1, Y. Elizabeth Moráles-García1, Rocío Bustillos-Cristales
1 y Jesús Muñoz-Rojas1*.
1 Benemérita Universidad Autónoma de Puebla, Centro de Investigaciones en
Ciencias Microbiológicas, Puebla pue. CP 72000. 2. Estación experimental del
Zaidin.
Abstract: An alternative for increasing the yield of crops is the use of PGPR, for this purpose
the colonization is a critical process. In this work the chemotaxis toward tomato plants and
bacteria colonization were evaluated for P. putida KT2440 and mutant strains in specific
chemoreceptors for GABA and succinato. Axenic seedlings of three days were placed at one
end of the recipients with soft agar (3g / L) and the opposite end vertically inoculated 100 µl
of the explored bacterial strain and the number of CFU/ml for 1-6 days after sowing was
recorded. At 3 days post-inoculation mcpG mutant showed less efficient colonization than
wild type and mcpS mutant. At 4 days post inoculation two mutant strains were affected in
colonization, while 6 days only mcpG-strain was affected. When the wild type and mutant
strains mcpG- were coinoculated in chemotaxis systems, again was observed decreased
colonization ability for the mutant strain. The mutation in the McpG and McpS
chemoreceptors affect the colonization process. Chemotaxis is vital for efficient rhizosphere
colonization which could have implications for growth promotion purposes.
Keywords: Chemotaxis, Colonization, McpS, McpG.
Corresponding author: J. Muñoz-Rojas
*joymerre@yahoo.com
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