ArticlePDF AvailableLiterature Review

Chili pepper as a body weight-loss food

  • Comenius University in Bratislava, Jessenius Faculty of Medicine
  • University of Oviedo. Asturias. Spain

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Chili has culinary as well as medical importance. Studies in humans, using a wide range of doses of chili intake (varying from a single meal to a continuous uptake for up to 12 weeks), concluded that it facilitates weight loss. In regard to this, the main targets of chili are fat metabolism, energy expenditure, and thermogenesis. To induce weight loss, the active substance of chili, capsaicin, activates Transient Receptor Potential Cation Channel sub-family V member 1 (TRPV1) channels) receptors causing an increase in intracellular calcium levels and triggering the sympathetic nervous system. Apart from TRPV1, chili directly reduces energy expenditure by activating Brown Adipose Tissue. Weight loss by chili is also the result of an improved control of insulin, which supports weight management and has positive effects for treatment for diseases like obesity, diabetes and cardiovascular disorders. This review summarizes the major pathways by which chili contributes to ameliorating parameters that help weight management and how the consumption of chili can help in accelerating weight loss through dietary modifications.
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International Journal of Food Sciences and Nutrition
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Chili pepper as a body weight-loss food
Sharon Varghese, Peter Kubatka, Luis Rodrigo, Katarina Gazdikova, Martin
Caprnda, Julia Fedotova, Anthony Zulli, Peter Kruzliak & Dietrich Büsselberg
To cite this article: Sharon Varghese, Peter Kubatka, Luis Rodrigo, Katarina Gazdikova, Martin
Caprnda, Julia Fedotova, Anthony Zulli, Peter Kruzliak & Dietrich Büsselberg (2016): Chili
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Chili pepper as a body weight-loss food
Sharon Varghese
, Peter Kubatka
, Luis Rodrigo
, Katarina Gazdikova
, Martin Caprnda
Julia Fedotova
, Anthony Zulli
, Peter Kruzliak
and Dietrich B
Weill Cornell Medicine in Qatar, Qatar Foundation-Education City, Doha, Qatar;
Department of Medical Biology, Jessenius Faculty of
Medicine, Comenius University in Bratislava, Martin, Slovakia;
Department of Gastroenterology, Faculty of Medicine, University of
Oviedo, Central University Hospital of Asturias (HUCA), Oviedo, Spain;
Department of Nutrition, Faculty of Nursing and Professional
Health Studies, Faculty of Medicine, Slovak Medical University, Bratislava, Slovakia;
Department of General Medicine, Faculty of
Medicine, Slovak Medical University, Bratislava, Slovakia;
2nd Department of Internal Medicine, Faculty of Medicine, Comenius
University and University Hospital, Bratislava, Slovakia;
Laboratory of Neuroendocrinology, I.P. Pavlov Institute of Physiology, Russian
Academy of Sciences, St. Petersburg, Russia;
Laboratory of Comparative Somnology and Neuroendocrinology, I.M. Sechenov Institute
of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia;
International Research Centre
Biotechnologies of the Third Millennium, ITMO University, St. Petersburg, Russia;
Centre for Chronic Disease, College of Health and
Biomedicine, Victoria University, Werribee, Australia;
Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary
and Pharmaceutical Sciences, Brno, Czech Republic;
Department of Surgery, Center for Vascular Disease, St. Annes University
Hospital, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Chili has culinary as well as medical importance. Studies in humans, using a wide range of doses
of chili intake (varying from a single meal to a continuous uptake for up to 12 weeks), concluded
that it facilitates weight loss. In regard to this, the main targets of chili are fat metabolism, energy
expenditure, and thermogenesis. To induce weight loss, the active substance of chili, capsaicin,
activates Transient Receptor Potential Cation Channel sub-family V member 1 (TRPV1) channels)
receptors causing an increase in intracellular calcium levels and triggering the sympathetic ner-
vous system. Apart from TRPV1, chili directly reduces energy expenditure by activating Brown
Adipose Tissue. Weight loss by chili is also the result of an improved control of insulin, which
supports weight management and has positive effects for treatment for diseases like obesity, dia-
betes and cardiovascular disorders. This review summarizes the major pathways by which chili
contributes to ameliorating parameters that help weight management and how the consumption
of chili can help in accelerating weight loss through dietary modifications.
Received 10 August 2016
Revised 1 November 2016
Accepted 3 November 2016
Chili pepper; weight loss;
appetite; energy balance
Chili and health
Chili is a widely used flavoring spice and is culturally
prominent in diets of various communities and cul-
tures around the world since 7000BC (Kraft et al.
2014). In chili, more than 200 constituents have been
identified and some of its active constituents play
numerous beneficial roles in the human organism.
The major active compounds of chili are the pungent
capsaicinoids (capsaicin and dihydrocapsaicin), which
give a hot sensation when consumed. Capsinoids, which
are non-pungent capsaicin analogs (capsiate, dihydro-
capsiate, and nordihydrocapsiate), are substances also
naturally present in chili peppers. Those peppers also
contain other components like antioxidants, vitamins,
and carotenoids (Maji & Banerji 2016).
Most attention is given to capsaicin especially in
regard its ability to modulate pain (Zhang & Li Wan Po
1994), e.g. by interacting with TRPV1 receptor channel
complex allowing calcium to enter the cytosol (Satheesh
usselberg 2015). Apart from the treatment of pain
inflammation, capsaicin is considered as an appropriate
substance applicable in the add-on therapy of rheuma-
toid arthritis (Richards et al. 2012), cluster headaches
(Matharu 2010), herpes zoster (Jeon 2015), and vaso-
motor rhinitis (Singh & Bernstein 2014).
The other main area of research investigates the
effects of chili intake on cardiovascular parameters
like heart rate, Subendocardial Viability Ratio (SEVR)
and Calcitonin Gene Related Peptide (CGRP). While
beneficial effects were reported (e.g. heart attacks and
CONTACT Peter Kruzliak Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and
Pharmaceutical Sciences, Palackehotr 1946/1, 612 42 Brno, Czech Republic; Dietrich B
usselberg Weill Cornell
Medicine in Qatar, Qatar Foundation-Education City, POB 24144, Doha, Qatar; Katarina Gazdikova Department of
Nutrition, Faculty of Nursing and Professional Health Studies, Faculty of Medicine, Slovak Medical University, Limbova 12, 833 03 Bratislava, Slovakia
Author's Agreement: The manuscript is approved by all named authors and the order of authors listed in the manuscript has been approved by all authors.
ß2016 Informa UK Limited, trading as Taylor & Francis Group
long-term tachycardia were reduced; a higher CGRP
contributing to vasodilation helped to combat arterial
blocks, Yoshioka et al. 2001; Ahuja & Ball 2006; Yuan
et al. 2015) other parameters like arterial stiffness,
inflammation, or oxidative stress biomarkers (Nieman
et al. 2012) were unchanged. Moreover, capsaicinoids
significantly decreased serum total cholesterol, low-
density lipoprotein cholesterol, and tri-acylglycerols
without affecting the high-density lipoprotein choles-
terol in the animal model. This effect was caused by
the stimulating conversion of cholesterol to bile acids
(Zhang et al. 2016). Chili demonstrated beneficial
effects in various gastrointestinal disorders such as
stimulation of digestion and gastro-mucosal defense,
reduction of gastroesophageal reflux disease symp-
toms, inhibition of gastrointestinal pathogens, ulcer-
ation and cancers, regulation of gastrointestinal
secretions, and absorptions (Maji & Banerji 2016).
Furthermore, capsaicin has proven an effective anti-
cancer agent. Several up-to-date preclinical studies
reported that capsaicin may suppress various human
neoplasia by generating reactive oxygen species and
increasing apoptosis (Sarkar et al. 2015; Liu et al.
2016; Zheng et al. 2016). Finally, capsaicin demon-
strated significant antioxidant and metal-binding
properties and therefore it was postulated that this
compound has important implications in the preven-
tion or treatment of neurodegenerative diseases such
as Alzheimers disease (Dairam et al. 2008).
This review focuses on a less highlighted aspect, in
particular how chili intake influences parameters
known to reduce body weight and how they are modi-
fied by the consumption of chili. A widespread health
concern is the large number of adults and children of
developed societies who are overweight. The World
Health Organization (WHO) (2014) reports that at
least 1.9 billion adults, 18 years and older, are over-
weight, and of those more than 600 million are obese
(Body Mass Index BMI above 30). Data show that
in 20112012, 16.9%of youth and 34.9%of adults in
the United States suffer from obesity (Ogden et al.
2014). Overweight and obesity result in heart disease,
diabetes, reduced longevity, psychological, and social
acceptance issues to name just a few.
Multiple approaches to reduce weight or stop gain-
ing weight, such as changes in lifestyle including phys-
ical exercise (U.S. Department of Health and Human
Services 2008) and diet control are most commonly
used to manage weight (Nurkkala et al. 2015).
Here, we summarize how dietary chili intake influ-
ences parameters such as metabolism or insulin levels
which directly or indirectly influence the energy
balance and therefore body weight (overview in
Figure 1).
Effects of chili on body weight
Overweight and obesity develop when the energy
intake (food consumption) exceeds the energy used by
the body. When the energy intake (EI) exceeds the
energy expenditure (EE) it is defined as a positive
energy balance. Measures to increase the expenditure
or reduce the intake are adopted for weight manage-
ment programs (Hill et al. 2012) along with elevating
diet induced thermogenesis(DIT) to change the
energy balance. Recent reviews (Ludy & Mattes 2011;
Whiting et al. 2014) showed strong evidence for sig-
nificant and positive effects of chili consumption in
aggravating energy metabolism. Unfortunately, not
many studies analyze how parameters like dosage of
chili, age group, ethnicity, weight, etc. are related to
intake of chili.
The following chapters give an overview of how
rates of oxidation, respiratory measurements, thermo-
genesis, energy expenditure and intake, appetite, and
insulin regulation are modified by chili consumption
under experimental and clinical settings (Tables 1
and 2).
Effect on rates of oxidation of lipids and
Capsaicin has been reported to increase energy
expenditure and diet-induced thermogenesis probably
due to b-adrenergic stimulation and a decrease in the
respiratory quotient, implying a shift in substrate oxi-
dation from carbohydrate to fat oxidation (Ludy &
Mattes 2011; Smeets et al. 2013; Shook et al. 2015).
Reduced lipoprotein oxidation and increased lipid oxi-
dation supports weight loss (Vasankari et al. 2001;
Berggren et al. 2008). This is asserted by lowered rate
of serum lipoprotein oxidation observed upon add-
ition of chili to diet. In women, a lag of this rate was
observed, which might be explained by a higher
amount of chili or capsaicin available per kg of body
weight. The study concluded that regular consumption
of chili resists serum lipoprotein oxidation (Ahuja &
Ball 2006). Addition of chili increased lipid oxidation
postprandial more in a high fat (HF) than a high
carbohydrate (HC) meal. Chili when consumed with
caffeine decreased lipid oxidation immediately after
the meal, whereas it increased when the subjects were
asleep (Yoshioka et al. 2001). Inclusion of capsaicin
was shown to maintain fat oxidation rates compared
with placebo (Lejeune et al. 2003). Addition of chili
postprandial decreased carbohydrate oxidation
(Yoshioka et al. 1998). Conversely, an increased carbo-
hydrate oxidation was reported in runners after the
consumption of chili, although this was not due to
increased energy expenditure (Lim et al. 1997). In a
recent study, Ohyama et al. (2015) examined whether
the beneficial effects of exercise could be enhanced by
capsinoids supplementation in mice. The combination
of exercise and capsinoid supplementation robustly
improved metabolic profiles, including the plasma
cholesterol level and significantly activated both the
oxidative phosphorylation and fatty acid oxidation in
skeletal muscle. This combination increased cyclic
Adenosine Mono-Phosphate (cAMP) levels and pro-
tein kinase A activity in brown adipose tissue, indicat-
ing an increase of lipolysis (Ohyama et al. 2015).
Moreover, this combination prevented diet-induced
liver steatosis and decreased the size of adipocyte cells
Table 1. Parameters influenced by chili consumption.
Factors affected Conclusion from studies References
Rates of oxidation Improved, even with non-pungent spices,
caffeine and green tea
Yuan et al. (2015), Zheng et al. (2016), Lee et al. (2015), Ahuja and
Ball (2006), and Snitker et al. (2009)
RQ and RER Decreased RQ and Increased RER Yuan et al. (2015), Zheng et al. (2016), Smeets et al. (2013), and Ludy
and Mattes (2011)
DIT & Energy Expenditure Improved DIT and increase by up to 30% Yuan et al. (2015), Zheng et al. (2016), Ohyama et al. (2015), Smeets
et al. (2013), and Ludy and Mattes (2011)
Energy intake Reduced intake of fat and protein uptake Yuan et al. (2015), Zhang et al. (2016), and Reyes-Escogido et al.
Appetite and satiety Reduced ghrelin levels Yuan et al. (2015), Zheng et al. (2016a, b), Smeets et al. (2013),
Smeets and Westerterp-Plantenga (2009), Ludy and Mattes (2011),
and Janssens et al. (2013)
Insulin Reduced blood glucose and increased
glucose tolerance
Li et al. (2014), Ahuja et al. (2007), Kraft et al. (2014), Larsen (2008),
Islam and Choi (2008), and Chen et al. (2015)
Figure 1. Overview of the pathways activated by chili consumption. The figure highlights the parameters affected by chili
(PURPLE), i.e. capsaicin and capsinoids (non-pungent), contributing to weight (TEAL) management. The RED pathway shows the
components of oxidative processes that directly involve in reducing the amount of fat available. The ORANGE pathway highlights
the activation and effects on brown adipose tissue (BAT) with the consumption of chili. Finally, the BLUE pathway combines the
mechanisms activated by chili under the control of the sympathetic nervous system, i.e. TRPV1 receptors, appetite, hormonal man-
agement of Insulin and Ghrelin. The pathways together summarize how and where in the body chili has the most effective regula-
tion in the management of weight.
in white adipose tissue. In another study, using obese
diabetic KKAy mice, dietary capsaicin increased the
expression of the adiponectin gene/protein and its
receptor (AdipoR2) in adipose tissue and/or plasma,
and these changes were accompanied by increased
activation of hepatic AMP-activated protein kinase, a
marker of fatty acid oxidation (Kang et al. 2011).
The fact that capsiate administration contributes to
the enhancement of aerobic ATP production and the
reduction of body fat content in rats (through a skel-
etal muscle mitochondrial uncoupling protein-3 gene
downregulation) was also confirmed (Faraut et al.
Chili increases fat oxidation and reduces triglycer-
ide accumulation, which is the main constituent of
body fat (pathway illustrated in RED in Figure 1).
Thus, their reduction can contribute to ameliorating
weight gain. Free fatty acids are formed by lipolysis of
triglycerides. Triglycerides are a target of the uncou-
pling protein 1 (UCP-1), which when upregulated and
in conjunction with Sympathetic Nervous System
(SNS) activation, causes thermogenesis (Figure 1). The
inclusion of chili in the diet primarily activates
TRPV1 receptors and triggers a number of pathways
that can result in a more efficient weight management.
TRPV1 increases [Ca
reducing adipogenesis and
reduced lipid accumulation (Figure 1; RED pathway).
This increase of [Ca
is mediated through
Connexin 43 (Cx 43). The upregulation of Cx 43
improves adipocyte-to-adipocyte communication
resulting in lipolysis and thus contributing to reduc-
tion in body fat and consequently weight loss (Chen
et al. 2015).
Interestingly the ingestion of non-pungent capsi-
noid also resulted in a significant increase in fat oxida-
tion (Snitker et al. 2009).
Effect on RQ and RER
The respiration quotient (RQ) and respiratory
exchange ratio (RER) are values to determine the
amount of CO
exhaled to oxygen. They are identical
at the resting state (Farlex Partner Medical Dictionary
2012). RQ is used for determining Basal Metabolic
Rate and measures the overall metabolism. The RER
helps in determining RQ as well as the fuel (carbohy-
drate or fat) used for metabolism at steady state. RER
is higher (Valtue~
na et al. 1997) and RQ is reduced
(Hainer et al., 2000) when losing weight. RQ is
inversely proportional to fat oxidation while reducing
triglyceride accumulation thereby contributing to
weight loss. Postprandial RQ was reduced by 30%
after a meal containing chili (Yoshioka et al. 2004;
Smeets et al. 2013). Reduced RQ was observed in diet-
ary consumption of chili among habitual users and
non-users of chili (Ludy & Mattes 2011). On the con-
trary, capsinoids administered in four doses (1, 3, 6,
and 12 mg) to 13 healthy subjects did not affect meta-
bolic rate and respiratory quotient when measured 2 h
after exposure. Authors suggested that longer exposure
and higher capsinoids doses may be required to cause
meaningful acute effects on energy metabolism
(Galgani et al. 2010). Another study investigated the
24 h effects of capsaicin on energy expenditure and
substrate oxidation during 25%negative energy bal-
ance. Capsaicin decreased the RQ in human subjects
with 75%of their daily energy requirements compared
to subjects without negative energy balance (Janssens
et al. 2013). Authors concluded that the consumption
of 2.56 mg capsaicin per meal in humans supported
negative energy balance by counteracting the unfavor-
able negative energy balance effect of decrease in com-
ponents of energy expenditure. One study reported
different results (an increase in RQ with the consump-
tion of chili), but, it remains unclear whether this
might be due to the fact that they specifically made
this investigation with "runners" (Lim et al. 1997). An
elevation of RER was also observed when chili and
caffeine were consumed simultaneously (Yoshioka
et al. 2001).
Chili elevates thermogenesis and energy
Between regular users and non-chili users, EE
increased more in non-chili users when chili was eaten
Table 2. Metabolic effects of chili consumption under experimental conditions.
Study performed Participants Dose of chili used Observations Conclusions
Kang et al. (2010) Male C57BL/6 mice 0.015% capsaicin for 10 weeks Enhanced expression of adiponectin
and receptor
Decreased fasting glucose/insulin
decreased triglyceride levels
Reduced metabolic
Ohyama et al. (2015) C57BL/6J mice 0.3% capsinoids Increased cAMP levels and PKA
activity in BAT
Increased Energy expenditure
via activation off at
Islam Choi (2008) SpragueDawley rats 0.5% and 2% red chili Increased serum insulin concentration Insulinotrophic action
(Matsumoto et al. 2000; Ludy & Mattes 2011). Chili
consumed simultaneously with caffeine or green tea
also increased EE (Yoshioka et al. 2001; Reinbach
et al. 2009). A reduced EE after a chili meal was noted
in subjects with BMI 26 and it was suggested that
this could be due to reduced postprandial insulin lev-
els (Ahuja et al. 2006).
Seven healthy volunteers were fed a breakfast con-
taining chili and medium-chain triglycerides (MCT)
oil, chili and sunflower oil, bell pepper and sunflower
oil or bell pepper, and MCT oil. Adding chili and
MCT to meals increased Diet Induced Thermogenesis
(DIT) by over 50%in observed subjects. Authors con-
cluded that this effect may cumulate to help induce
weight loss and prevent weight gain or regain (Clegg
et al. 2013). It has been documented that chili con-
sumption increases DIT in a HC and HF diet
(Yoshioka et al. 1998,2004) but was more pronounced
in persons who generally did not use chili in their diet
(Ludy & Mattes 2011). The core body temperature
increased after chili and CH-19 Sweet consumption by
0.02 C, independent of whether the persons were
regular chili users or not (Ohnuki et al. 2001; Ludy &
Mattes 2011). The largest changes were recorded
1060 min after intake (Ohnuki et al. 2001). But, on
the contrary, chili could also reduce the core body
temperature (Chatsantiprapa et al. 2014) and the skin
temperature (Ludy & Mattes 2011). The temperature
drop depends on the form of chili that was consumed,
i.e. capsule form or chili as a whole. These differences
might be the result of variations between individuals
as they are not easily explained.
Chili affects energy expenditure by triggering the
Brown Adipose Tissue (BAT) in the same way as low
temperature does, leading to increased energy expend-
iture via non-shivering thermogenesis through TRPV1
channels (Saito & Yoneshiro 2013; Saito 2014).
Thermogenesis is achieved through two pathways,
which increase uncoupled mitochondrial respiration
and secretion of catecholamine from the adrenal
medulla (Westerterp-Plantenga et al. 2006; Reyes-
Escogido et al. 2011). The consumption of a non-pun-
gent compound of chili (CH-19 Sweet) increases
thermogenesis after consumption (Ohnuki et al. 2001).
This can be attributed to an indirect pathway via fat
oxidation, which also generates heat (to the same
pathway as for capsinoids in Figure 1) (Snitker et al.
Thus, the stimulation of the SNS by chili results in
an increase of noradrenaline hormone, causing the
activation of b-adrenergic receptors found in adipo-
cytes. The sympathetic stimulation of the BAT by lip-
olysis and increased intracellular concentration of fatty
acids increases uncoupled respiration and, therefore,
the up-regulated Uncoupling Protein-1 (UCP-1) in
mitochondria of BAT, resulting in a rise of tempera-
ture as mentioned earlier (see ORANGE colored path-
way in Figure 1). The SNS stimulation is responsible
for the catecholamine secretion from the adrenal
medulla contributing to thermogenesis. Capsaicin and
its non-pungent analog capsinoids as known agonists
for TRPV1 have the potential to increase whole-body
energy expenditure and reduce body fat. When indi-
viduals without active BAT were exposed to cold every
day for 6 weeks, BAT was recruited in association
with increased energy expenditure and decreased body
fat. Importantly, a 6-week daily ingestion of capsinoids
mimicked the effects of repeated cold exposure on
BAT (Yoneshiro & Saito 2013).
Chili reduces appetite
Decreased energy intake is attributed to an increase in
the ratio of sympathetic (SNS) to parasympathetic
(PSNS) nervous system activity (Bray 1993). Elevated
SNS activity most likely may be the reason for the
increased heart rate and systolic blood pressure, as
seen in chili consuming group. There are no reports
in the literature on the parasympathetic nervous sys-
tem activity. Therefore, the increasing effect on the
SNS: PSNS ratio is mainly attributed to the SNS
Chili increases anorexigenicity (Janssens et al. 2014)
and chili users were not only less hungry but also had
a reduced desire to eat fatty, salty and sweet foods.
This later effect is more prominent in irregular con-
sumers of chili than habitual consumers (Ludy &
Mattes 2011). Chili reduced the desire to eat, tested in
a HF and a HC diet with the effect being more pro-
nounced in the HF diet. Adding chili increased the
sensation of oiliness thereby resulting in satiety
(Yoshioka et al. 1998; Yoshioka et al. 1999). Addition
of chili to breakfast meal decreased the protein uptake
with both diets. When a chili-based appetizer was pro-
vided between breakfast and lunch, ad libitum energy
intake was decreased (Yoshioka et al. 1999; Ludy &
Mattes 2011; Janssens et al. 2014).
The sensation of fullness and increased satiety was
observed in the positive balanced state but not in the
negative balance state (Janssens et al. 2014). These
findings were contradicted by other researchers, who
found a stronger effect on appetite at the negative
energy state (Reinbach et al. 2009). While Smeets and
Westerterp-Plantenga (2009) initially reported no sig-
nificant difference in satiety, they later observed a
decrease in appetite when they replaced carbohydrates
by proteins (Smeets et al. 2013).
Supplementing a chili meal with caffeine (Yoshioka
et al. 2001) or green tea (Yoshioka et al. 2001;
Reinbach et al. 2009) reduced energy intake with
respect to control as well as the desire to eat fatty,
salty and hot foods. Chili on its own and with caffeine
results in a significant reduction in energy intake,
which was observed in two independent studies by
Yoshioka et al. (1999,2001). Although there was no
combined study to observe comparisons between
them, both studies separately showed significantly
reduced significant energy intakes. Meanwhile, reduc-
tion in energy intake was more pronounced when chili
was combined with green tea than with chili or green
tea independently (Reinbach et al. 2009).
Chili activates TRPV1, which increases glucagon
like peptide-1 (GLP-1) protein levels via stimulated
SNS and consequently reduces ghrelin (a hunger and
energy state-related hormone) levels in the gut
(Larsen 2008; Smeets & Westerterp-Plantenga 2009).
This causes a decrease in appetite, which leads to
decreased energy intake due to a feeling of satiety.
Overall, the correlation among sensation of fullness,
satiety, or appetite with energy intake is well
Insulin regulation
Insulin is a hormone secreted by the bcells of the
islets of Langerhans and regulates glucose levels in
blood. Insulin resistance (IR) occurs when cells do not
respond to insulin, and glucose is not metabolized,
causing an increased blood glucose level. High-glucose
levels will cause bcells to produce more insulin and if
not metabolized will cause hyperinsulinemia. Insulin
resistance is closely associated with obesity and they
are directly proportional to each other (Kahn et al.
IR is frequently measured using the Homeostasis
Model Assessment/Insulin Resistance (HOMA/IR)
quotient (which are calculated using fasting glucose
and insulin levels). Improved insulin management
with chili consumption can be attributed to the
increase in GLP-1 production via TRPV1-mediated
calcium increase (BLUE pathway in Figure 1).
Consumption of chili reduces insulin resistance as a
decrease is observed 2-h in the postprandial HOMA/
IR in a chili eating group (Li et al. 2014) and in preg-
nant women (Yuan et al. 2015). Overproduction of
insulin caused by a meal could result in IR. Chili
intake reduces this overproduction and, therefore,
reducing the risk of IR.
Habitual consumption of chili helps in relieving
meal-induced hyperinsulinemia, as lower serum insu-
lin concentrations (Ahuja et al. 2006) and decreased
postprandial insulin levels over time in obese partici-
pants were observed (Kroff et al. 2015). A higher
GLP-1 level triggered by the activation of TRPV1
receptors by capsaicin explains the improved regula-
tion of glucose homeostasis and its tolerance resulting
in lower postprandial insulin levels. This conclusion is
supported by the observation that TRPV1 knockout
mice have a higher insulin resistance (Lee et al. 2015).
Diaz-Garcia et al. (2014) concluded that TRPV1 does
not contribute to glucose-induced insulin secretion in
beta cells as was previously thought, but it is possible
that it may control insulin sensitivity. Another study
showed that capsaicin, independent of insulin,
increases glucose uptake via ROS generation and con-
sequent AMPK and p38 MAPK activations (Kim et al.
Insulin resistance can lead to diabetes, but diabetes
can also be caused by decreased insulin levels. In the
second case, the supplementation of chili results in
decreased glucose and increased insulin levels. A
decrease in 2-h postprandial plasma glucose (Yuan
et al. 2015) and glucose levels over a period of time
(Kroff et al. 2015) were observed in chili consuming
groups. A study on mice, with two doses of chili add-
ition to meals, showed decreased fasting blood glucose
levels in low chili consuming groups (Islam & Choi
2008). Reduction in plasma glucose and insulin level
maintenance was also observed (Chaiyasit et al. 2009)
with the addition of chili. The reduction observed in
blood glucose levels can also be drawn as a conse-
quence of increased GLP-1 levels with TRPV1 medi-
ated [Ca
increase. A more recent study showed
that capsaicin-containing chili supplementation regu-
larly improved postprandial hyperglycemia and hyper-
insulinemia as well as fasting lipid metabolic disorders
in women with gestational diabetes mellitus, and it
decreased the incidence of large-for-gestational-age
newborns (Yuan et al. 2015).
Various targets of weight management are affected
with the consumption of chili directly and indirectly
as research indicates. There is a difference with regard
to the effects between regular consumers and non-
users of chili as regular users may be desensitized.
Interestingly, it is hypothesized that this desensitiza-
tion maybe due to reduced SNS activation, which is
also a characteristic of obesity (Ludy & Mattes 2011;
Ludy et al. 2012). Although there are different
observations (Table 3) in the parameters measured,
these contradictions can be attributed to a number of
factors especially experimental conditions. Some
experiments were conducted on mice and dogs while
most of the studies were on human subjects with vary-
ing populations of different physiological conditions
such as age, gender, ethnicity, pregnancy state, and
physical exercise. Most groups used chili in a meal
whereas some used capsaicin capsules. The type of
chili, the dosage, and the duration of treatment within
each experiment were also different. When chili was
used in a meal, the presence of other bioactive com-
pounds may also be a factor to consider the difference
in readings. For example, differences in the subjects,
like sensitivity to consumption and amount of intake
of chili, etc. all affect resting energy states and metab-
olism and the consumption of chili would only add to
the complexity if the above parameters are not nor-
malized for all the studies so far. If different subjects
are tolerant to different amounts and kinds of chili,
there is a need to set a standard control on each fac-
tor. All these variations may have caused distinguish-
ing trends in some of the parameters discussed above,
such as respiration, temperature, energy intake, and
Insulin regulation.
It is established that athletes have a lower resting
heart rate and the difference between the RQ levels
observed in runners who consumed chili included
meals, can be hypothesized due to the sustained
higher oxygen requirement in athletes.
Reduced body temperature recorded by
Chatsantiprapa et al. (2014), even if statistically insig-
nificant, was similar to the significantly reduced tem-
perature recorded by Ahuja and coworkers (Ahuja
et al. 2006,2007). Although the data were insignificant
in the case of Chatsantiprapa et al., the significant
reduction described by Ahuja and team may be due to
other physiological processes, like anxiety of experi-
menting or other psychological stress that cause
reduction in temperature.
Studies found a decrease in energy intake by
including chili in diet. The results of a single study
that did not show this could be due to the difference
in combination of bioactive compounds affecting the
presence of capsaicin in the system. The presence of
caffeine and bioactive substances in green tea showed
reductions in energy balances but with various
combinations and in different experiments. A com-
bined experiment is required to see the actual
improvement with the combination and if they have a
better effect together than independently. The effects
of chili consumption are similar to the effects induced
by certain other bioactive compounds found in caf-
feine and components of green tea like tea catechins.
The consumption of the combination suggests stron-
ger effects on the parameters measured statistically
and needs further research to clarify significant reduc-
tions in appetite or energy intake.
No observable difference was shown in a study by
Ahuja et al. (2006) of the acute effects of insulin and
blood glucose levels with the consumption of chili,
contradictory to other studies that demonstrated
improved insulin homeostasis with chili. An increase
in insulin level was shown in a high chili dose group
in mice (Islam & Choi 2008). Insulin levels are a deli-
cate balance when both extremes cause an imbalance
in the form of diabetes as hyperinsulinemia. An
increase in the insulin levels would be an advantage in
a diabetic scenario where as detrimental in the latter.
Thus, the predisposed state of the subjects needs to be
considered carefully when consuming chili.
Despite many advantages of chili consumption,
caution is advised on their uncontrolled intake, for
example unfortunate deaths in 2008 and 2013 were
reported when two Caucasian men died after the con-
sumption of an unknown concentration of chili. Such
incidents point to limits on the consumption of chili.
Hypothetically, the deaths may have been caused due
to a disruption in the SEVR or an immediate and pro-
longed tachycardia leading to cardiac arrest.
Further research is necessary to determine the key
dosage and form of intake of chili to better assess its
true potential in weight management. Currently avail-
able data, on non-pungent capsinoids and capsaici-
noinds, can serve as a harbinger in developing a
variant suitable for non-habitual consumers of chili
who are not comfortable with the feeling of hotness
but can still benefit by its use. It is very difficult to
define an ideal dosage as it depends on the circum-
stances and differs from individual to individual. It
can even vary with various parameters such as fre-
quency of consumption, sensitivity to spice, combina-
tions with other bioactive compounds, metabolism,
energy balance, level of fitness, and presence or
Table 3. Studies which report results different from the general observations.
Factors affected Contradictions References
RQ Increased RQ in runners, contrary to other studies Lim et al. (1997)
Temperature Contradictory views on temp Dairam et al. (2008) and Janssens et al. (2014)
Insulin and blood glucose No significant differences Dairam et al. (2008) and Islam and Choi (2008)
absence of other health concerns. Furthermore, if the
possibility of chili as a conqueror for obesity is taken
into consideration, the concept of ideal dosage of chili
may extrapolate into the field of personalized medi-
cine. For best results, the ideal dosage hence is crucial,
meanwhile if the consumption of chili causes any
physical discomfort; it is advisable to seek medical
Overall, dietary chili intake can help in regulating fac-
tors that favor weight loss. At this juncture, the ideal
dosage needed to significantly contribute to weight
loss and safe consumption still warrants further
research. But consumption of chili is not a substitu-
tion to regular physical exercise or controlled dietary
Disclosure statement
The authors report that they have no conflicts of interest.
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... Painful burn caused by capsaicin triggers an inflammation-redness of the tissue and localised increase of temperature similar to the reaction to a light burn. At the same time, capsaicinoids and capsinoids can affect the central and peripheral sympathetic nervous system through an action on production of neurohormones and neuromediators (substance P, serotonin and somatostatin) (Rollyson et al. 2014;Fernandes et al. 2016;Srinivasan 2016;Varghese et al. 2017;Uarrota et al. 2021). ...
... It reduces acidity and increases secretion in the gastrointestinal tract, and therefore, Capsicum is suitable for prevention and treatment of stomach ulcers (Srinivasan 2016). It activates intracellular insulin and glucagon, decreases levels of blood sugar (Zhang et al. 2017;Uarrota et al. 2021;Wang et al. 2022b) and reduces the symptoms of metabolic syndrome and diabetes (Varghese et al. 2017;Sanati et al. 2018;Wang et al. 2022b). It improves the metabolism of carbohydrates: it redirects glycogen accumulation from the liver (where it is stored) to muscles (where it is burned) (Kim et al. 2018). ...
... Large-scale multicentric experiments validated that consumption of capsaicinoids reduces weight, abdominal fat stores, appetite and food consumption. Therefore, capsaicinoids can be an efficient ingredient in food with added value and food supplements applicable in programmes focused on weight reduction and improvement of metabolism (Kawabata et al. 2006;Reinbach et al. 2009;Whiting et al. 2014;Clegg et al. 2013;Varghese et al. 2017;Irandoost et al. 2021). In contrast to pepper and capsaicin, the performed clinical studies did not reveal the significant influence of non-pungent capsinoids on human weight and obesity (Marlatt and Ravussin 2017;Elmaz and Gezer, 2022;Szallasi 2022). ...
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Phytotherapy can be an efficient tool for prevention and treatment of disorders including obesity. The purpose of this narrative review is to summarize the available knowledge concerning the positive effects of peppers (Capsicum spp.) and their alkaloid capsaicin on human health, in particular on fat and obesity. Search for literature was performed in Medline/Pubmed, Web of Science and SCOPUS databases between the year 2000 and 2023. Words used to search were pepper, Capsicum, capsaicin, review, obesity, fat, weight loss and mechanisms. The available data demonstrate that both pepper extract and capsaicin can positively influence human health and treat several disorders. Moreover, they can reduce fat storage affecting brain centres responsible for the sensation of hunger, nutrient uptake by gastrointestinal tract, state of adipocytes, increase in carbohydrate and fat oxidation, metabolism and thermogenesis and other mechanisms. Therefore, despite some possible limitations, these substances could be useful for treatment of obesity.
... Additionally, there are methods to treat the metabolic syndrome, which includes obesity, diabetes, and cardiovascular disease. 30 Additionally, the capsaicin and quercetin found in chili pepper fruit significantly reduced hyperglycemia in an animal model. 31 According to what we discovered, extraction of "Bang Chang" chili pepper from ethanol, and because it has biological properties like antioxidant and anti-inflammation, as well as being non-pungent, it can be used externally for medicinal purposes like as a muscle relaxant and massage oil to reduce subcutaneous fat. ...
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Chili peppers (Capsicum spp.) are really important crops in several Asian countries. In Thailand, chili pepper cultivars are also a wide range of flavors, colors, shapes, and spiciness levels. The cultural importance in Thai cuisine. The “Bang Chang chili pepper” (Capsicum annuum var. acuminatum), a Thai cultivar of capsicum, had first cultivated in Bang Chang subdistrict, Samut Songkhram, Thailand. This study aimed to determine capsaicin, phenolic compounds and flavonoids and screen ethanol extract’s biological activities. The extract’s average capsaicin and total phenolic content (TPC) was 10.4 ± 0.3 (g/100 mL and 2.50 ± 0.13 mg GAE/g, while it could not determine flavonoids. The low amount of capsaicin in extract was defined this cultivars as non-pungent capsicum (0-700 Scoville Heat Units, SHU). This extract was strongly scavenged NO and DPPH radicals (IC50 = 0.09 ± 0.02 and 14.88 ± 1.59 mg/mL). There was also exerted in-vitro anti-inflammation activity by suppression of albumin breakdown (IC50 = 0.51 ± 0.05 mg/mL), which, can comparable to control, diclofenac diethyl ammonium (IC50 = 0.45 ± 0.01 mg/mL). Therefore, lipid peroxidation was weakly inhibited (IC50 >1,000 mg/mL) and unable to trap metals compared to vitamin E and EDTA. According to our finding, this extract was exerted biological properties like antioxidant and anti-inflammation, as well as being non-pungent, it can be used externally for medicinal purposes like as a muscle relaxant and massage oil to reduce subcutaneous fat. This extract can be used as a condiment in Asian recipes and other healthful dishes
... Scientific evidence indicates that red pepper can improve blood circulation and gastric abnormalities [101] while ameliorating neuralgia and rheumatism [102,103]. Capsaicin and its derivatives are effective against abdominal pain, bloating [104], and pain [105][106][107], as well as alleviating other complications that underlie diabetes and overweight [108]. In addition, it has been shown that capsaicin alone has anti-inflammatory [109] and antioxidant [110][111][112] properties. ...
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The consumption of food-derived products, including the regular intake of pepper, is increasingly evaluated for its potential benefits in protecting against diverse metabolic complications. The current study made use of prominent electronic databases including PubMed, Google Scholar, and Scopus to retrieve clinical evidence linking the intake of black and red pepper with the amelioration of metabolic complications. The findings summarize evidence supporting the beneficial effects of black pepper (Piper nigrum L.), including its active ingredient, piperine, in improving blood lipid profiles, including reducing circulating levels of total cholesterol, low-density lipoprotein cholesterol, and triglycerides in overweight and obese individuals. The intake of piperine was also linked with enhanced antioxidant and anti-inflammatory properties by increasing serum levels of superoxide dismutase while reducing those of malonaldehyde and C-reactive protein in individuals with metabolic syndrome. Evidence summarized in the current review also indicates that red pepper (Capsicum annum), together with its active ingredient, capsaicin, could promote energy expenditure, including limiting energy intake, which is likely to contribute to reduced fat mass in overweight and obese individuals. Emerging clinical evidence also indicates that pepper may be beneficial in alleviating complications linked with other chronic conditions, including osteoarthritis, oropharyngeal dysphagia, digestion, hemodialysis, and neuromuscular fatigue. Notably, the beneficial effects of pepper or its active ingredients appear to be more pronounced when used in combination with other bioactive compounds. The current review also covers essential information on the metabolism and bioavailability profiles of both pepper species and their main active ingredients, which are all necessary to understand their potential beneficial effects against metabolic diseases.
... Based on the research conducted by Bertao et al., (2016), the results of a longitudinal study spanning three years and including a sample of 1983 individuals aged 19 to 70 years indicated that those with higher total antioxidant capacity scores saw a decrease in weight and abdominal circumference. Multiple studies have demonstrated the anti-obesity properties of red chili pepper via various mechanisms, such as thermogenesis, satiety, fat oxidation (Azlan et al., 2022) , and increased energy expenditure (Varghese et al., 2017). Additionally, a Malaysian study by Huei et al., (2020) that investigated the anti-obesity properties of local chilies varieties suggests that chili peppers exhibit anti-obesity properties. ...
Purpose: Although the use of chili peppers as functional foods in Lesotho is not well documented, their production and consumption have a rich historical background. Functional foods are those that serve as therapeutic agents in addition to nutrition. Previous research supports the many health benefits of consuming chili peppers, including their potential to mitigate the risk of cancer, cardiac arrhythmias, rheumatism, and chest colds. Nevertheless, these concepts have yet to translate into tangible alterations in policies and implementation strategies. Therefore, this review aims to identify potential topics for future research on Chili peppers, food, and therapeutic food product development; to improve the population's health and well-being Methodology: This paper presents a literature analysis of existing data on chili peppers as functional foods, specifically emphasizing their applicability to Lesotho, a developing country. Findings: Based on the analysis of the reviewed research, it can be inferred that those who regularly incorporate chili peppers into their diet exhibit a decreased risk of death from non-communicable diseases compared to those who do not. Unique Contribution to Theory, Practice and Policy: The data presented in this review supports the classification of chili pepper as a functional food while also emphasizing the importance of investigating the effectiveness, safety, quality, development, and possible hazards of chili peppers, particularly in developing nations like Lesotho. The present study has great potential to provide novel global perspectives on the associations between chili peppers, non-communicable diseases (NCDs), and mortality to guide future research. Hence, future research endeavors must thoroughly examine the distinct impacts of chili peppers through more clinical studies. Moreover, the present review and further research will contribute to revising Lesotho’s dietary guidelines and advancing functional food products, such as herbal supplements derived from chili peppers.
... The modulation of weight gain through the endocannabinoid system, mainly through the antagonism of cannabinoid receptors, has already been widely reported even in the clinic, with the drug rimonabant, which has already been approved but is currently no longer in circulation due to its induction of depression and anxiety (Le Foll et al., 2013). Despite the non-property of CBD to antagonise such receptors, studies focusing on the inverted U-shaped dose-response curve of this compound show that higher doses of CBD, when administered, can activate the transient receptor potential vanilloid subfamily 1 (TRPV1), the latter also participating in enhancing metabolism and energy expenditure, a possible explanation for this effect (Linares et al., 2019;Baskaran et al., 2017;Varghese et al., 2017;Zheng et al., 2017;Campos et al., 2012). ...
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Objectives In view of the neuroprotective characteristic of cannabidiol (CBD) and its beneficial action on aversive memory in non-diabetic animals, we aimed to investigate in animals with experimentally induced type-1 diabetes mellitus (T1DM) whether CBD treatment would be able to impair the contextual fear memory consolidation, its generalization and whether the effect would be lasting. We also investigated the CBD effect on anxiety-like responses. Methods After T1DM induction, animals received single or more prolonged treatment with CBD and were submitted to the contextual fear conditioning (CFC) test. As expression of activity-regulated cytoskeletal-associated (Arc) protein is necessary for memory consolidation, we evaluated its expression in the dorsal hippocampus (DH). For evaluating anxiety-related responses, animals were submitted to the elevated plus maze test (EPMT), in which the time and number of entries in the open arms were used as anxiety index. Results A single injection of CBD impaired the contextual fear memory consolidation and its generalization, which was evaluated by exposing the animal in a neutral context. This single injection was able to reduce the elevated expression of Arc in the DH from these animals. Interestingly, more prolonged treatment with CBD also impaired the persistence of context-conditioned fear memory and induced an anxiolytic-like effect, as the treated group spent more time in the open arms of the EPMT. Conclusion CBD interferes with contextual fear memory and the dosage regimen of treatment seems to be important. Moreover, we cannot rule out the involvement of emotional aspects in these processes related to fear memory.
... Consumption of chili peppers can modulate energy metabolism to induce numerous health benefits. The bioactive ingredients responsible for the spiciness and beneficial effects of chili peppers are capsaicinoids including capsaicin (CAP) and dihydrocapsaicin (DHC) [1,2]. However, there are two major drawbacks to the therapeutic use of capsaicinoids that should be considered. ...
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Background Capsaicinoids, such as dihydrocapsaicin (DHC), exert the health-promoting effects of chili peppers on energy metabolism. The metabolic responses to capsaicinoids are primarily mediated through transient receptor potential cation channel subfamily V member 1 (TRPV1). However, the varying contributions of their metabolites to beneficial health outcomes remain unclear. 8-methyl nonanoic acid (8-MNA), a methyl-branched medium chain fatty acid (MCFA), is an in vivo degradation by-product of DHC. Since MCFAs have emerged as metabolic modulators in adipocytes, here we examined various cellular responses to 8-MNA in 3T3-L1 adipocytes. Methods The viability of 3T3-L1 adipocytes exposed to various concentrations of 8-MNA was assessed by the Calcein AM assay. Biochemical assays for lipid accumulation, AMP-activated protein kinase (AMPK) activity, lipolysis and glucose uptake were performed in 3T3-L1 adipocytes treated with 8-MNA during 48-h nutrient starvation or 5-day maturation. Results 8-MNA caused no impact on cell viability. During nutrient starvation, 8-MNA decreased lipid amounts in association with AMPK activation, a molecular event that suppresses lipogenic processes. Moreover, 3T3-L1 adipocytes that were treated with 8-MNA during 5-day maturation exhibited a reduced lipolytic response to isoproterenol and an increased glucose uptake when stimulated with insulin. Conclusions These results suggest that 8-MNA derived from DHC modulates energy metabolism in adipocytes and also support the idea that the metabolic benefits of chili consumption are partly attributable to 8-MNA.
... These compositions could confer many health benefits, including anti-mutagenic, and anti-tumour-promoting activities (Aboul-Enein et al. 2012). Additionally, it has been used for inducing weight loss (Varghese et al. 2017), treating low-grade inflammation (Qin et al. 2017), controlling blood cholesterol (Dubey 2017) and improving the function of the digestive system (Sachan et al. 2018). Topical capsaicin has been shown to have a safe analgesic effect against many painful conditions such as burning mouth syndrome (Jørgensen & Pedersen 2017), idiopathic trigeminal neuralgia (Fusco & Alessandri 1992), neuropathic pain (Raber et al. 2015) and osteoarthritis (Guedes et al. 2018). ...
Plant cell culture technology serves as an effective alternative system for in vitro production of bioactive molecules, as it allows for the exploration of valuable compounds under a controlled environment. The present study was conducted to evaluate the effect of plant growth regulator (PGR); 2,4-dichlorophenoxyacetic acid (2,4-D) on the expression of compounds in coloured callus of Capsicum frutescens, a vital spice in various cuisines worldwide. The differential accumulation of compounds in the callus was analysed using liquid chromatography-mass spectrometry (LCMS) and the PGR concentration that resulted in the highest accumulation of the valuable compounds was identified. In this study, calli of various colours (cream, yellow and green) were successfully produced from C. frutescens through plant tissue culture. The increase in 2,4-D concentrations was found to increase callus growth index (GI) and specific growth rate (Sg), where the highest GI (0.5690) and Sg (0.6348 mg/week) were observed in callus produced in media supplemented with 0.5 mg/L 2,4-D. LCMS data analyses showed that 19 compounds were detected in the callus, with 8 compounds (fatty acids and phenolics) were successfully identified, while the remaining 11 compounds were reported as unknowns. Yellow-coloured callus was observed to contain the highest number of compounds (18 compounds), while green callus contained the least (14 compounds). This analysis provides valuable information on the application of biotechnological tools such as plant tissue culture as an alternative for sustainable production of compounds with high bioactivity in Capsicum frutescens.
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So‘ngi yillarda dunyoning ko‘plab mamlakatlari iqtisodiy jihatdan istiqbolli potensialga ega qalampir o‘simligini yetishtirish hamda an’anaviy va zamonaviy tibbiyotdagi ahamiyani o‘rganishga e’tibor qaratmoqda. Ushbu maqolada madaniylashtirilgan qalampir (Capsicum) turlarining botanik tavsifi, farmakologik xususiyatlari, an’anaviy va zamonaviy tibbiyotda turli kasalliklarni oldini olish hamda davolovchi ta’siridan foydalanish bo‘yicha olib borilgan ilmiy-amaliy tadqiqot natijalari, oziq-ovqat sanoatida ishtaha ochuvchi muhim ziravor sifatidagi ahamiyati, dunyoning turli mintaqalarida uni yetishtirishga oid statistik ma’lumotlar hamda fitokimyoviy tarkibini o‘rganish bo‘yicha olib borilgan ilmiy tadqiqot ishlari muhokamasi bayon qilingan.
Red chili pepper is a beneficial natural spicy food that has antiobesity and antitype II diabetes effects, but it is not conducive to in-depth research as a dietary strategy to treat obesity. This study aims to investigate the beneficial effects of red chili pepper, fermented with a novel Lactococcus lactis subs. cremoris RPG-HL-0136. LC-MS/MS analysis is conducted to detect the content of capsaicin and dihydrocapsaicin, and no significant difference is observed between the nonfermented red chili pepper (NFP) (W/W) and the prepared L. lactis subs. cremoris RPG-HL-0136-fermented chili mixture (LFP). After establishing a high-fat diet-induced obese type II diabetic mouse model, the effects on weight gain, weight loss of liver and testicular fat, total cholesterol, triglyceride, fasting glucose, insulin, and homeostatic model assessment for insulin resistance in LFP were evaluated to be better than those in NFP following 10 weeks of interventions. All animal experiments were approved by the Institutional Animal Care and Use Committee of Xinxiang medical university. NFP and LFP could increase the expression of transient receptor potential vanilloid subfamily 1, peroxisome proliferator-activated receptor-alpha and caspase-2 in the high-fat mice. Compared with unfermented red chili pepper, the fermented red chili pepper complex significantly reduced LPS, tumor necrosis factor-alpha, and interleukin-6 in serum (P < .05). Intake of LFP significantly increased the expression of claudin-1 and occludin in the colon of the high-fat mice (P < .05), and there was no damage to the stomach and colon. This study provides scientific evidence that red chili pepper, fermented with L. lactis subs. cremoris RPG-HL-0136, may be beneficial for future treatment of obesity and accompanying diabetes. (IACUC.No.XYLL-20200019).
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The recurrence of bladder cancer after surgery with or without chemotherapy remains a major challenge in bladder cancer treatment. Previous studies have shown that transient receptor potential vanilloid 1 (TRPV1) acts as a tumor suppressor through inducing apoptosis in bladder cancer cells. However, whether activation of TRPV1 has any synergistic effects with pirarubicin (THP), one of main drugs used in urinary bladder instillation chemotherapy to improve chemotherapeutic efficacy has remained elusive. The present study verified that TRPV1 was differentially expressed in bladder cancer cell lines. Furthermore, activation of TRPV1 by capsaicin was shown to induce growth inhibition of 5637 cells in which TRPV1 was highly expressed, while the growth of T24 cells, which express TRPV1 at low levels, was not affected. In addition, the present study demonstrated that activation of TRPV1 enhanced the anti‑proliferative effects of pirarubicin using an MTT assay and cell cycle analysis. Finally, immunofluorescent microscopy revealed that activation of TRPV1 prevented the translocation of proliferating cell nuclear antigen to the nucleus. This phenomenon was reversed by pre‑treatment with capsazepine, a specific TRPV1 antagonist. In conclusion, the present study confirmed the anti‑tumor activity of TRPV1 against bladder cancer. Activation of TRPV1 may be applied as a novel strategy to treat bladder cancer or enhance the therapeutic efficacy of traditional chemotherapeutic drugs.
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A growing body of evidence suggests that capsaicin ingestion may lead to desirable metabolic outcomes; however, the results in humans are equivocal. Whether or not benefits may be gained from ingestion of capsaicin via a commercially available meal has not been determined. The objectives of this randomised, cross-over intervention study were to compare the 2 h postprandial effects of a standard commercially prepared meal containing chilli (HOT, 5·82 mg total capsaicinoids) with a similar meal with no chilli (CON, <1·0 mg total capsaicinoids) on resting energy expenditure, plasma insulin, glucose, serum high sensitivity C-reactive protein (hs-CRP) concentrations, core body temperature and forearm microvascular reactivity responses in overweight individuals. A total of thirty-four apparently healthy individuals (sixteen men and eighteen women) between 18 and 50 years of age, with a BMI >25 kg/m 2 and a waist circumference >94 cm (men) or 80 cm (women), were studied. Participants had normal glucose tolerance and were accustomed, but were not regular chilli eaters. A paired t test indicated that insulin AUC was smaller following the HOT meal ( P =0·002). Similarly, there was a tendency for glucose AUC to be reduced following the HOT meal ( P =0·056). No discernable effects of the HOT meal were observed on metabolic rate, core temperature, hs-CRP concentrations and endothelial-dependent microvascular reactivity. The results from this study indicate that a standard restaurant meal containing a relatively small dose of capsaicin delivered via African bird’s eye chilli, which is currently available to the public, results in lower postprandial insulin concentrations in overweight individuals, compared with the same meal without chilli.
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In recent years, the role of capsaicin in cancer prevention and treatment has gained people's attention. However, the mechanism of anti-glioma cells by capsaicin has not been elucidated. Here, we discuss the mechanism of capsaicin in U251 cells. Cell viability was detected by MTT and extracellular LDH measurements, while immunofluorescence was performed to measure changes of LC3 in U251 cells. The expressions of LC3II, Puma-α, Beclin1, P62, Procaspase-3, and P53 were observed by immunoblotting. The cell viability decreased and the punctate patterns of LC3 in U251 cells were observed after Capsaicin treatment. Meanwhile, the expressions of Beclin1, P62, and Puma-α increased. After using 3-MA, the expressions of Beclin1 and Procaspase-3 were reduced while those of P53 and Puma-α increased. The expression of LC3II was increased after Pifithrin-α treatment. Therefore, we believed that capsaicin could induce apoptosis in U251 cells, and the inhibition of autophagy could contribute to apoptosis.
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Herpes zoster (HZ) is a transient disease caused by the reactivation of latent varicella zoster virus (VZV) in spinal or cranial sensory ganglia. It is characterized by a painful rash in the affected dermatome. Postherpetic neuralgia (PHN) is the most troublesome side effect associated with HZ. However, PHN is often resistant to current analgesic treatments such as antidepressants, anticonvulsants, opioids, and topical agents including lidocaine patches and capsaicin cream and can persist for several years. The risk factors for reactivation of HZ include advanced age and compromised cell-mediated immunity (CMI). Early diagnosis and treatment with antiviral agents plus intervention treatments is believed to shorten the duration and severity of acute HZ and reduce the risk of PHN. Prophylactic vaccination against VZV can be the best option to prevent or reduce the incidence of HZ and PHN. This review focuses on the pathophysiology, clinical features, and management of HZ and PHN, as well as the efficacy of the HZ vaccine.
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To investigate if chili consumption as a food ingredient in a fried rice meal would have any real acute pharmacological effects on autonomic nervous system (ANS) and metabolic system (MS), a randomized, cross-over, intervention trial was conducted in 33 healthy subjects. The study found that a moderate dose of chili (containing 16.96 - 24.40 mg capsaicin plus 4.86 - 6.99 mg dihydrocapsaicin) given in a meal significantly increased systolic blood pressure and decreased rMSSD (the square root of mean of sum of square of successive difference) parameter of heart rate variability reflecting a reduced parasympathetic function of the ANS. Chili also affected MS by significantly reducing the body temperature, but not other MS parameters. Time-passing also had highly significant effects on ANS and MS. However, the linear mixed modeling revealed that there was little evidence that chili modified the physiological response over a 180 min period. This result suggests that the body's strong physiologic responses modulate any chili effects to the extent that there are minimal differential responses over the experimental period. These findings confirmed that moderate amount of chili in food have significant acute effects on the modulation of ANS and MS but not therapeutic effect on weight reduction.
Dietary spices and their active constituents provide various beneficial effects on the gastrointestinal system by variety of mechanisms such as influence of gastric emptying, stimulation of gastrointestinal defense and absorption, stimulation of salivary, intestinal, hepatic, and pancreatic secretions. Capsicum annuum (Solanaceae), commonly known as chilli, is a medicinal spice used in various Indian traditional systems of medicine and it has been acknowledged to treat various health ailments. Therapeutic potential of chilli and capsaicin were well documented; however, they act as double-edged sword in many physiological circumstances. In traditional medicine chilli has been used against various gastrointestinal complains such as dyspepsia, loss of appetite, gastroesophageal reflux disease, gastric ulcer, and so on. In chilli, more than 200 constituents have been identified and some of its active constituents play numerous beneficial roles in various gastrointestinal disorders such as stimulation of digestion and gastromucosal defense, reduction of gastroesophageal reflux disease (GERD) symptoms, inhibition of gastrointestinal pathogens, ulceration and cancers, regulation of gastrointestinal secretions and absorptions. However, further studies are warranted to determine the dose ceiling limit of chilli and its active constituents for their utilization as gastroprotective agents. This review summarizes the phytochemistry and various gastrointestinal benefits of chilli and its various active constituents.
Background/objectives: Metabolic disturbances, such as reduced rates of fat oxidation (high respiratory quotient (RQ)) or low energy expenditure (low resting metabolic rate (RMR)), may contribute to obesity. The objective was to determine the association between a high RQ or a low RMR and changes in body weight and body composition over 1 year. Subjects/methods: We measured RQ and RMR in 341 adults using indirect calorimetry, along with body weight/body composition using dual-energy X-ray absorptiometery, energy expenditure using an arm-based activity monitor and energy intake using dietary recalls. Participants were classified into low, moderate or high RQ and RMR (adjusted for age, sex, race and body composition) groups according to tertiles by sex. Follow-up measurements were completed every 3 months. Results: Individuals with a high RQ had larger gains in body weight and fat mass compared with individuals with a low/moderate RQ at month 3, and increases in fat mass were more than double among individuals with a high RQ at 12 months (1.3±3.0 vs 0.6±3.7 kg, P=0.03). Individuals with a low RMR did not gain more body weight nor fat mass compared with individuals with a moderate/high RMR. Conclusion: The primary finding is a high RQ is predictive of gains in body weight and fat mass over a 12-month period among young adults, with changes occurring as soon as 3 months. In addition, a low RMR was not associated with gains in body weight or fat mass over the same period.European Journal of Clinical Nutrition advance online publication, 25 November 2015; doi:10.1038/ejcn.2015.198.
Background: Loss of function of the p53 gene is implicated in defective apoptotic responses of tumors to chemotherapy. Although the pro-apoptotic roles of eugenol and capsaicin have been amply reported, their dependence on p53 for apoptosis induction in gastric cancer cells is not well elucidated. The aim of the study was to elucidate the role of p53 in the induction of apoptosis by eugenol and capsaicin in a human gastric cancer cell line, AGS. Materials and methods: AGS cells were incubated with or without various concentrations of capsaicin and eugenol for 12 hrs, in the presence and absence of p53 siRNA. Cell cycling, annexin V and expression of apoptosis related proteins Bax, Bcl-2 ratio, p21, cyt c-caspase-9 association, caspase-3 and caspase-8 were studied. Results: In the presence of p53, capsaicin was a more potent pro-apoptotic agent than eugenol. However, silencing of p53 significantly abrogated apoptosis induced by capsaicin but not that by eugenol. Western blot analysis of pro-apoptotic markers revealed that as opposed to capsaicin, eugenol could induce caspase-8 and caspase-3 even in the absence of p53. Conclusions: Unlike capsaicin, eugenol could induce apoptosis both in presence and absence of functional p53. Agents which can induce apoptosis irrespective of the cellular p53 status have immense scope for development as potential anticancer agents.