ArticlePDF Available

Mixture of olive oil and Aloe vera gel: A natural mosquito repellent and a skin moisturizer collected from district Kohat, KP, Pakistan

Authors:

Abstract and Figures

Aloe vera is also called a succulent plant belonging to Order Asparagales, family sphodelaceae and genus Aloe and found in arid areas and conserves water. Aloe vera leaves are fleshy having gel like substance inside. Olive oil is also being used since ancient times for skin care and smoothening agent for skin. Human skin is prone to be invaded by mosquitos and other vectors that can transmit so many diseases like Dengue, Malaria, Zika virus, Arbovirus and others. Fresh Aloe vera plant was collected from its natural habitat i.e. Hangu road grave yard in district Kohat, Pakistan. Leaves were properly cut i.e. their hard epidermis was peeled off by sharp knife and the inner gel was scooped out by a spoon. Gel was kept in a glass jar for further experimental analysis. Refined olive oil was purchased from local market of district Kohat. Olive oil was mixed with the Aloe vera gel and sample was prepared in different concentrations. The mixture was applied to the skin by volunteers overnight. It was revealed in this study that Aloe vera gel and Olive oil have a mosquito repellent property when mixed. Mixture can be prepared with different concentration as per skin sensitivity keeping in mint the allergic reactions of the Aloe vera gel. As one’s skin can be allergic to the Aloe vera. Moreover, Olive oil has skin smoothening property it can keep skin moisturized.
No caption available
… 
Content may be subject to copyright.
~48~
International Journal of Mosquito Research 2016; 3(4): 48-49
ISSN: 2348-5906
CODEN: IJMRK2
IJMR 2016; 3(4): 48-49
© 2016 IJMR
Received: 08-05-2016
Accepted: 09-06-2016
Shehzad Zareen
Department of Zoology, Kohat
University of Science and
Technology-26000, KPK,
Pakistan
Shahid Niaz Khan
Department of Zoology, Kohat
University of Science and
Technology-26000, KPK,
Pakistan
Muhammad Adnan
Department of Botany, Kohat
University of Science and
Technology-26000, KPK,
Pakistan
Hameed Ur Rehman
Department of Chemistry, Kohat
University of Science and
Technology-26000, KPK,
Pakistan
Muhammad Ateeq
Department of Biotechnology,
University of Sargodha
Kausar Saeed
Department of Zoology
AWKUM Buner Campus
Waqar Ahmad
Department of Chemistry
Islamia College University
Peshawar
Munsif Ali Khan
Department of Zoology, Shankar
Campus, AWKUM, Pakistan.
Feroz Khan
Department of Zoology, Pir
Mehr Ali Shah Arid Agriculture
University Islamabad Pakistan.
Raqeebullah
Dept. of Zoology AWKUM,
Pakistan.
Correspondence
Shehzad Zareen
Department of Zoology, Kohat
University of Science and
Technology-26000, KPK,
Pakistan
Mixture of olive oil and Aloe vera gel: A natural
mosquito repellent and a skin moisturizer
collected from district Kohat, KP, Pakistan
Shehzad Zareen, Shahid Niaz Khan, Muhammad Adnan, Hameed Ur
Rehman, Muhammad Ateeq, Kausar Saeed, Waqar Ahmad, Munsif Ali
Khan, Feroz Khan and Raqeebullah
Abstract
Aloe vera is also called a succulent plant belonging to Order Asparagales, family sphodelaceae and genus
Aloe and found in arid areas and conserves water. Aloe vera leaves are fleshy having gel like substance
inside. Olive oil is also being used since ancient times for skin care and smoothening agent for skin.
Human skin is prone to be invaded by mosquitos and other vectors that can transmit so many diseases
like Dengue, Malaria, Zika virus, Arbovirus and others. Fresh Aloe vera plant was collected from its
natural habitat i.e. Hangu road grave yard in district Kohat, Pakistan. Leaves were properly cut i.e. their
hard epidermis was peeled off by sharp knife and the inner gel was scooped out by a spoon. Gel was kept
in a glass jar for further experimental analysis. Refined olive oil was purchased from local market of
district Kohat. Olive oil was mixed with the Aloe vera gel and sample was prepared in different
concentrations. The mixture was applied to the skin by volunteers overnight. It was revealed in this study
that Aloe vera gel and Olive oil have a mosquito repellent property when mixed. Mixture can be prepared
with different concentration as per skin sensitivity keeping in mint the allergic reactions of the Aloe vera
gel. As one’s skin can be allergic to the Aloe vera. Moreover, Olive oil has skin smoothening property it
can keep skin moisturized.
Keywords: Aloe vera, Olive oil, mosquito repellent, skin moisturizer
1. Introduction
Aloe vera is also called a succulent plant belong to Order Asparagales, family sphodelaceae
and genus Aloe found in arid areas and conserve water [1]. Aloe vera leaves are fleshy having
gel like substance inside [2]. Geographical distribution of this plant is worldwide [3]. Studies
suggested that its gel is being used in cosmetics [4]. It has also moisturizing and wound healing
property [5]. Aloe vera is now being used as herbal drug for infertilities of skin [6]. Olive oil is
also being used from long ago for skin care and smoothening agent for skin [7]. Our skin is
prone to be invaded by mosquitos and other vectors, that can transmit so many diseases like
Dengue, Malaria, Zika virus, Arbovirus and others [8, 9, 10] keeping in views all ground realities
of victor borne diseases the current study is designed to explore herbal technique to prevent toe
attack of disease causing vector i.e. mosquito.
2. Materials and methods
2.1 Plant Collection / Gel extraction
Fresh Aloe vera plant was collected from its natural habitat i.e. Hangu road grave yard in
district Kohat, Pakistan. Leaves were properly cut i.e. their hard epidermis was peeled off by
sharp knife and the inner gel was scooped out by a spoon. Gel was kept in a glass jar for
further experimental analysis.
2.2 Purchase of Oil
Refined olive oil was purchased from local market of district Kohat.
2.3 Sample Preparation
Olive oil was mixed with the Aloe vera gel and Sample was prepared in different
concentrations.
~49~
International Journal of Mosquito Research
3. Results
In first step Aloe vera gel was extracted from fresh plants and
was blended to make a homogenate. In second step 200 ml
Olive oil was taken in each of the three beakers and Aloe vera
gel was mixed in it as shown in the table. In first beaker only 1
table spoon gel was mixed, in 2nd beaker 2 table spoon gel was
mixed while in 3rd beaker 3 table spoons gel was mixed. All
the beakers were blended discretely for 10 minute in an
electric blender.
S. No. Olive oil
(ml) Gel
(Table spoons) Blended time
(Minute) Volunteers Results
From male respondents Results
From female respondents
1 - - - 1 female - Mosquito attack at night
2 200 1 10 1 male,
1 female Few mosquito bites, smooth skin Few mosquito bites, smooth skin
3 200 2 10 1 male,
1 female No mosquito bites, smooth skin No mosquito bites, smooth skin
4 200 3 10 1 male,
1 female No mosquito bites, smooth skin No mosquito bites, Skin irritation
3.1 Experimentation
Experiment was performed on humans with a prior informed
consent as there is no harmful effect of olive oil as well as
Aloe vera gel on human skin. Both are most commonly used
for treating skin infirmities. About 6 volunteers were included
in an experiment to massage this mixture oil on the naked area
of their body overnight and spend night in backyard of the
house to check the incidence of mosquito attack. 1 volunteer
spent night without applying the oil as he was considered to be
a control group of this experiment. Volunteer who didn’t apply
anything, reported mosquito bites at night. In 2nd group
volunteers reported few mosquito bites at night, more over
they reported their skin smoother at morning then earlier. In
third group volunteers reported no mosquito bite at night and
they felt their skin very smooth and moisturized. There was a
bit concern in fourth group, members of fourth group reported
no mosquito bite but female reported a bit skin irritation due
for few minutes, which later on disappeared. The study was
approved by the Departmental Ethics Committee at the
Department of Zoology, Kohat University of Science and
Technology, Pakistan, according to the Helsinki Convention
World Medical Association (WMA).
4. Conclusions
In 2016, S. Zareen conducted study on Larvicidal activity of
medicinal plant Eucalyptus Leaf extracts against Anopheles
mosquitoes collected from district Kohat and concluded the
results that Eucalyptus leaf extracts have a larvicidal activity
against larvae of Anopheles mosquitoes. No larva was found
dead till 7 hour of observation when distilled water was
applied into Petri dishes. Larvicidal activity can be enhanced
by applying maximum concentration of leaf extracts along
with an extended time frame.
The present study revealed that Aloe vera gel and Olive oil
have a mosquito repellent property when mixed. Mixture can
be prepared with different concentration as per skin sensitivity
keeping in mint the allergic reactions of the Aloe vera gel. As
one’s skin can be allergic to the Aloe vera. Moreover, Olive oil
has skin smoothening property it can keep skin moisturized.
5. Recommendations
Keeping in mind the out breaks Malaria, Dengue and other
infections caused by mosquito vector, we invite further studies
to be carried out on Aloe vera gel and Olive oil regarding their
mosquito repellent property so that world can get rid of such
deadly diseases.
6. Acknowledgments
This investigation was a continuous study of the Ph.D degree
of Mr. Shehzad Zareen, Department of Zoology, Kohat
University of science and technology, Kohat
7. References
1. Perkins, Cyndi. Is Aloe a Tropical Plant? SFgate.com.
Retrieved 13 Feb. 2016.
2. Yates A. Yates Garden Guide. Harper Collins Australia,
2002.
3. Aloe vera. African flowering plants database.
Conservatoire et Jardin botaniques de la Ville de Genève.
Retrieved 2008-06-20.
4. Boudreau MD, Beland FA. An Evaluation of the
Biological and Toxicological Properties of Aloe
Barbadensis (Miller), Aloe Vera. J Environ Sci Health C
Environ Carcinog Ecotoxicol Rev. 2006; 24(1):103-54.
doi:10.1080/10590500600614303. PMID 16690538.
extract in various solvents has variable but significant
antibacterial activity against the five clinical isolates used
in this study.
5. Kunkel G. Plants for Human Consumption. Koeltz
Scientific Books. ISBN 3-87429-216-9, 1984.
6. Quattrocchi, Umberto. CRC World Dictionary of
Medicinal and Poisonous Plants: Common Names,
Scientific Names, Eponyms, Synonyms, and Etymology
(5 Volume Set) CRC Press. ISBN 978-1420080445, 2012.
7. Grossman AJ. Behind a Mysterious Balm, a Self-Made
Pharaoh. The New York Times. 2007. Retrieved April 5,
2013
8. Mosquitoes of Michigan-Their Biology and Control.
Michigan Mosquito Control Organization. 2013.
9. Gates, Bill. The Deadliest Animal in the World.
10. Would it be wrong to eradicate mosquitoes? - BBC News.
BBC News. Retrieved.
11. Zareen S, Khan SN, Adnan M, Rehman HU, Asim M,
Sana MA et al. Larvicidal activity of medicinal plant
Eucalyptus Leaf extracts against Anopheles mosquitoes
collected from district Kohat, Journal of Entomology and
Zoology Studies. 2016; 4(4):966-967.
ResearchGate has not been able to resolve any citations for this publication.
Article
Full-text available
Eucalyptus is one of the most diverse genuses among flowering trees with multiple stem. It mainly dominates Australia as it has more than 700 species found over there. Fresh leaves of Eucalyptus were collected from their natural habitat, that they were crushed and converted into powder form. To obtain plant extract, that powder was dissolved in different solvents, an artificial pond was designed at KDA region of district Kohat in bushy and semi green area where mosquitoes were considered to be in large number. Experiments were performed in 7 assays of Petri dishes in 4 steps; each Petri dish was filled with equal amount of water. About 20 healthy/active larvae were placed in every Petri dish at each step. A total of 140 larvae were used in each step (20 Larvae X 7 Petri dishes = 140 assays). Eucalyptus leaf extracts have a larvicidal activity against larvae of Anopheles mosquitoes. No larva was found dead till 7 hour of observation when distilled water was applied into Petri dishes. Larvicidal activity can be enhanced by applying maximum concentration of leaf extracts along with an extended time frame.
Article
Aloe barbadensis (Miller), Aloe vera, has a long history of use as a topical and oral therapeutic. The plant is the source of two products, gel and latex, which are obtained from its fleshy leaves. Aloe vera products contain multiple constituents with potential biological and toxicological activities, yet the active components elude definition. Ingestion of Aloe vera is associated with diarrhea, electrolyte imbalance, kidney dysfunction, and conventional drug interactions; episodes of contact dermatitis, erythema, and phototoxicity have been reported from topical applications. This review examines the botany, physical and chemical properties, and biological activities of the Aloe vera plant.
Is Aloe a Tropical Plant? SFgate
  • Cyndi Perkins
Perkins, Cyndi. Is Aloe a Tropical Plant? SFgate.com. Retrieved 13 Feb. 2016.
African flowering plants database
  • Aloe Vera
Aloe vera. African flowering plants database. Conservatoire et Jardin botaniques de la Ville de Genève. Retrieved 2008-06-20.
Behind a Mysterious Balm, a Self-Made Pharaoh. The New York Times
  • A J Grossman
Grossman AJ. Behind a Mysterious Balm, a Self-Made Pharaoh. The New York Times. 2007. Retrieved April 5, 2013
The Deadliest Animal in the World
  • Bill Gates
Gates, Bill. The Deadliest Animal in the World.