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Biological Nanomechanics: ATP Synthesis and Deuterium Depletion
Abstract
At ~1 deuterium/6600 protons natural abundance the ATP synthase protein nanomotor may break down in every few seconds without effective deuterium depleting biochemical processes through glycolysis and the TCA cycle. This talk explains how deuterons damage mitochondrial ATP synthase nano-mechanics by severely compromising proton on-off loading onto the Asp61 C-protein residue of the FO rapidly (9000/min) rotating nano-motor subunit. This is based on the ~1500 proton/second transfer velocity of the rotating protein. Alike, there are deuterium-induced isotope effects caused by its low dissociation constant from asparagine and its high mass to co-regulate the TCA cycle by malate dehydrogenase during oxaloacetate formation. This is because in malate dehydrogenase there are also Asp168 and several arginine residues that participate in proton binding, stabilization and transfer reactions. The inherent complexities of glycolysis and the TCA cycle is explained by their primary role in deuterium depletion, as well as multiple exchanges with metabolic water in the cytoplasm coming from the mitochondrial matrix. In simple terms glycolysis acts as a metabolic “dryer” to rip off all extracellular and extramitochondrial deuterium and hydrogen atoms from glucose, while the TCA cycle acts as a metabolic “washer” by adding hydrogen atoms back from low deuterium metabolic matrix water to specific carbons to be oxidized in the TCA cycle. Therefore, natural ketogenic substrate oxidation via deuterium depleted matrix water production becomes a critical resource for mitochondrial health. On the other hand, the Warburg effect and serine oxidation with glycine cleavage (SOGC) are deuterium loading alternative energy producing pathways and are the result of excessive deuteronation of the mitochondrial matrix and intermembrane space. In conclusion, excessive deuterium loading is involved in isotopic breakdowns of ATP synthase, malate dehydrogenase and thus TCA cycle nanomechanics, which require mitochondrial repairs with important adaptive changes in cellular metabolism. These may produce the Warburg effect for lactate efflux due to insufficient pyruvic acid oxidation, even in the presence of oxygen at intolerable deuterium levels in diseased mammalian cells. The talk offers mechanisms how deuterium becomes an oncoisotope and how global warming, for example, may be a contributor to increased cancer incidence worldwide due to limited deuterium fractionation during water cycling in Nature. Talk: https://youtu.be/6P8gqB4zLGQ
... These fatty acids are also needed to maintain the production of low deuterium "metabolic water" production in other organs. 15 Therefore, careful inhome heart rate measurements offer a technique for the monitoring of potential toxic deuterium exposure from foods and drinking water. ...
... Mass spectroscopy does indicate that deuterium is more concentrated in the plant storage sugars, e.g. carbohydrates, as compared to fats 15 suggesting these drops in heart rates are due to systemic deuterium depletion. Figure 3. Peak exercise heart rates measured on steep climbs versus age. ...
Mitochondrial cardiac ATP production efficiency in beef hearts has been known to decrease with increasing systemic deuterium 2H levels since the 1980’s. Recently ketogenic diets which are known to decrease deuterium levels in humans were found to decrease both the resting and exercise heart rates in a volunteer. Furthermore, resting heart rates were found to systematically vary with the deuterium content from the previous meal consumed by six volunteers. A cardiac model is proposed showing extreme sensitivity of heart rate on the deuterium loading of the ATP synthase nanomotors. A predicted increase in heart rate by 28% is expected with a 5% decrease in ATP production. This finding strongly suggests that high deuterium levels in the fatty acids contribute to the diastolic dysfunction in heart failure not already attributed to direct structural damage, i.e., heart failure with preserved ejection fraction.
... 14;17 In sum, there is a total of 6 NADH and 2 FADH2 that are produced inside the mitochondria matrix and these provide the deuterium depleted hydrogens needed to efficiently drive the ATPase nanomotors. 17 Any contamination of the mitochondria matrix by deuterium isotopes seriously reduce the efficiency of the ATPase nanomotors in producing cellular ATP. [1][2][3] An estimate of hepatic ATP production efficiency in our patient can be estimated from the slopes in the fasting glucoses vs total sleep duration trends taken from Figure 4. ...
A 59-year-old patient with a 18-year history of type-II diabetes is presented who showed dramatic improvements to glucose tolerance tests and increased fasting hepatic glucose production with systemic deuterium depletion. Deuterium, which is well known to decrease the efficiency of the ATP syntheses nanomotors, is likely the mechanism leading to the systemic changes to both insulin and hepatic glucose production in the pancreas and liver, respectively. Systemic deuterium depletion occurs with consumption of low carbohydrate (keto) diets and deuterium depleted water.
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