Measurements took place at Aalborg University Copenhagen, Denmark, in an anechoic chamber.
Recruitment of the subjects was via social media and e-mails. All subjects were asked to complete
two therapeutic blind test sessions, each one lasting approx. 60 minutes together with the
measurements: during the first session they were listening to Melocura, a specially designed
sound therapy of certain frequency – 432 Hz (Melocura, Denmark) and during the second session
a normal relaxing piano music was played. Subjects sat on the edge of the flat bed, placed inside
the anechoic chamber, and were initially measured using the mfBIA unit as explained earlier, after
which an AMG recording was made for both muscles – here the subjects were asked to extend
their arms to approx. 90 degrees from their torso and to press the palms of their hands together 3
times, thereafter they were asked to bend forward 3 times as far as they could comfortably
manage. Subjects were measured two times at each session – before and after listening to sound
therapy or normal relaxing music (NRM). The purpose was to observe, if there were any
differences in muscle response to the NRM or Melocura sounds.
The pulse was recorded for each subject Pre and Post both sound tests using a ChoiceMMed Pulse
Oximeter (Bristol PA 19007, USA).
The bioimpedance data were analyzed for the Centre Frequency (fc) and the Extracellular
Resistance (Re), both of which were determined from the Cole-Cole plot. The intracellular
resistance (Ri) was calculated from the formula: Ri = (Re x R∞/Re-R∞). Membrane Capacitance
(Mc) was also calculated from the formula: fc = 1/(2π x Mc x (Re + Ri). A detailed analysis was
finally performed at 50 kHz with measurement of Resistance (R) and Reactance (Xc), and a
calculation of the Impedance (Z), where Z = Square Root (R2 + Xc2
). Finally, so as to be able to
compare between individuals of different body mass, the Phase Angle (PA) was calculated: PA =
arctan (Xc/R) with units in degrees. The mfBIA parameters were interpreted in terms of muscle
mass (Z, R), energy storage capacity/fibre size (Xc), hydration status (R, Re), tissue density/resting
tension (fc), membrane activity/integrity (Mc) and metabolic activity (Ri).
The AMG signal was analyzed in terms of its individual components Efficiency (E-score) and fibre
recruitment, Temporal (T-score) and Spatial (S-score) summation, which are all mean values made
for periods of physical activity, as outlined earlier.
All statistics were performed using GraphPad InStat 3 for Mac (Version 3.0b, 2003; Graph- Pad
Inc., La Jolla, CA). Data were initially tested for normal distribution and equal variance, and then
subsequently analyzed using an unpaired t-test. Differences between means with a P value > 0.05
were considered non-significant. Values are presented as the mean ± the standard error of the
The data obtained from the mfBIA measurements for both the neck and back muscles can be seen
in Table 1. It was found that subjects measured pre and post the two music tests, showed similar
mfBIA parameters, which did not prove to be statistically significant from each other.
The data obtained from the Acoustic Myography unit for both the neck and back muscles can be
seen in Table 2. It was found that Melocura alone had a significant effect on both the E-score as
well as the overall combined ESTi score. The overall ESTi score change was a 10.4% and 11.1%
improvement, respectively compared with Pre- test values. Pulse:
The Pulse measurements for the subjects in the Melocura test were; Pre 73.77 20.92 bpm and
Post 67.15 16.45 bpm. For the normal relaxing music (NMR) test the values were; Pre 74.65
20.57 bpm and Post 65.50 21.75 bpm. For both tests the resting values were very similar and the
Post values fell by 8% and 12%, respectively compared with Pre values. There was no significant
difference between the two tests, nor any effect of treatment, despite a clear trend towards and
fall in the pulse of the 26 subjects with both tests.