Twenty-seven recreational male and female athletes (25.9±5.9 years), with experience in resistance
training, were randomly assigned to 1of 3 groups: hydrolysed beef protein (B;n=9), whey protein (W;
n=9), or non-protein isoenergetic carbohydrate (maltodextrin; CHO; n=9). They all had to take 20gof
supplement (protein or carbohydrate) mixed with 250 ml of orange juice once a day (immediately after
workout or before breakfast) during the intervention period, an 8-week resistance-training programme
consisting of 3 training sessions per week. Measurements were performed pre- and post-intervention on
salivary alpha-defensins (HNP1-3) as reference of humoral immunity, body composition, using
plethysmography, jump performance and blood markers of health (total cholesterol, LDL and HDL
cholesterol, urea, uric acid, creatinine, alanine transaminase, aspartate transaminase).
Figure 1: Schematic overview of study design and training protocol (RT circuit).
F: familiarization sessions; WU: warm up; S1-2-3: sets; CD: cold down; SA-d: salivary alpha-defensins (HNP1-3);
BC: body composition; JP: jump performance; BMH: blood markers of health.
1Department of Life and Sports Science, School of Science, University of Greenwich, United Kingdom.
2Clinical Research Institute. Texas Tech University Health Science Center, Lubbock, TX, USA.
3Department of Pharmaceutical, Chemical & Environmental Sciences. University of Greenwich, United Kingdom.
EFFECTS OF COMBINING BEEF, WHEY OR CARBOHYDRATE SUPPLEMENTATION WITH RESISTANCE
TRAINING ON BODY COMPOSITION, AND MARKERS OF HEALTH IN COLLEGE ATHLETES
Marcos Seijo1, Eneko Larumbe2, Nadia Ashrafi3, Birthe Nielsen3, Fernando Naclerio1
Only the beef condition showed a significant intragroup decrease
in the HNP1-3 from pre- to post-intervention (p=0.025;d=1.03)
(Figure 2). No other significant differences between groups were
observed in body composition, jump performance or blood
markers of health.
Results from the present study demonstrated that supplementation with a carbohydrate-protein (beef or whey) after workout or before breakfast may support resistance-training
outcomes in a comparable way to the ingestion of only carbohydrate. Beef protein supplementation consumed with orange juice would also promote a decrease of the baseline levels
of the salivary HNP1-3 with no adverse effects on blood markers of health.
Figure 2: alpha-defensins HNP1-3 from pre- to post-intervention.
Beef is a nutrient-rich, high-quality protein source containing all the essential amino acids in proportions similar to those found in human skeletal muscle. The current study
examined the impact of ingesting hydrolysate beef protein, whey protein and carbohydrate on salivary alpha-defensins (HNP1-3), body composition, jump performance and blood
markers of health in college athletes, after an 8-week resistance-training programme.
MEATPROT and The University of Greenwich are providing joint funding to the completion of this project, however this does not affect this original
research content and purpose.
Authors declare that they have no conflicts of interest relevant to the content of this manuscript.
Conflicts of interest: