The sensitivity of the standardized ASCE grass-reference Penman-Monteith evapotranspiration ASCE-PM ET o equation to climate variables in different regions has not yet been studied. Sensitivity analyses for the standardized daily form of the ASCE-PM equation were conducted on wind speed at 2 m height U 2 , maximum and minimum air temperatures T max and T min , vapor pressure deficit VPD, and solar radiation R s in the following regions of the United States: semiarid Scottsbluff, Nebraska, and Bushland, Texas, a Mediterranean-type climate Santa Barbara, California, coastal humid Fort Pierce, Florida, inland humid and semihumid Rockport, Missouri, and Clay Center, Nebraska, and an island Twitchell Island, California. The sensitivity coefficients were derived for each variable on a daily basis. In general, ET o was most sensitive to VPD at all locations, while sensitivity of ET o to the same variable showed significant variation from one location to another and at the same location within the year. After VPD, ET o was most sensitive to U 2 in semiarid regions Scottsbluff, Clay Center, and Bushland during the summer months. The R s was the dominant driving force of ET o at humid locations Fort Pierce and Rockport during the summer months. At Santa Barbara, the sensitivity of ET o to U 2 was minimal during the summer months.. A 1 m · s −1 increase in U 2 resulted in 0.42, 0.18, 0.37, 0.28, 0.31, 0.20, and 0.26 mm increases in ET o at the same locations. A unit increase in T max resulted in the largest increase in ET o at Bushland, and a unit increase in R s caused the largest increases in ET o at Fort Pierce. A 1 MJ m −2 ·d −1 increase in R s resulted in 0.05, 0.08, 0.06, 0.11, 0.05, 0.06, and 0.06 mm increases in ET o at the same locations. A 0.4 kPa increase in VPD resulted in 1.13, 0.54, 1.29, 0.57, 1.04, 1.10, and 1.22 mm increases in ET o at the same locations. The U 2 had the most effect on ET o at Scottsbluff and Bushland, the two locations where dry and strong winds are common during the growing season. The sensitivity coefficient for R s was higher during the summer months and lower during the winter months, and the opposite was observed for VPD except for Twitchell Island. The decrease of the sensitivity coefficients for R s corresponding to an increase in the sensitivity coefficient for VPD is due to a decrease in the energy term in favor of the increase in significance of the aerodynamic term of the standardized ASCE-PM equation in summer versus winter months. Because the ASCE-PM and the Food and Agriculture Organization paper number 56 Penman-Monteith FAO56-PM equations are identical when applied on a daily time step, the results of the sensitivity analyses and sensitivity coefficients of this study should be directly applicable to the FAO56-PM equation.