This work carried out to investigate the impact of adding natural bioactive mixture juice composed of lemon, onion and garlic (LOG) juice on growth performance, mortality rate, carcass characteristics, some blood constituents, ceacum parameters and microbial activity. A total number of three hundred Rex rabbits aged 4 to 5 weeks (772±22 g) were divided into four equal comparable experimental groups (75 in each). Feeding trial lasted for 8 weeks (56 days) and the first experimental group rabbits received the basal diet that formulated to cover the requirements of growing rabbits and considered as (control group), meanwhile, drinking water was offered ad libtum without containing any additives (Zero LOG). On the other hand, the 2 nd ; 3 rd and 4 th groups were also fed the basal diet; meanwhile drinking water was added at levels 10, 20 and 30 ml LOG/ liter of drinking water for the 2 nd ; 3 rd and 4 th groups, respectively. The results showed that inclusion LOG at different levels significantly (P<0.05) increased average daily gain (ADG) compared to control. This increasing reaching to 13.7, 24.6 and 22.2% for 10, 20 and 30 ml LOG, respectively in comparison with control. Daily dry matter intake (DMI) was not affected by adding LOG at different levels, the average daily DMI was varied from 94 to 98 g/head/day among the different experimental group rabbits. Feed conversion that significantly (P<0.05) improved with adding LOG at different levels and through out the 2 nd week, 4 th week, 6 th week and 8 th week compared to control. Mortality rate was decreased with increasing the level of LOG addition. Inclusion LOG at different level significantly increased both carcass weight (CW) and Dressing percentages (DP). Increasing level of LOG increased values of red blood cells, white blood cells, hemoglobin, total protein, Albumin, globulin and albumin: globulin ratio were significantly (P<0.05) increased. Meanwhile it significantly (P<0.05) decreased values of blood NH3, blood urea, GOT, GPT, total lipids, phospholipids, triglycerides, HDL-cholesterol and total cholesterol. Caecal pH insignificantly decreased with adding LOG in drinking water up to 20 ml LOG/ liter drinking water. Meanwhile, adding LOG at different levels significantly (P<0.05) decreased total volatile fatty acids (TVFA's) concentration and fungi counts comparing to the control. Total bacteria counts was significantly (P<0.05) increased with increasing the level adding of LOG. On the other hand, inclusion LOG at different levels had no significant effect on both actinomyces and cellulolytic bacteria counts. Adding LOG decreased total aerobic bacterial counts (TABC), total Coliform counts (TCC), feacal coliform counts (FCC), total fungi counts (TFC) and total entero bacteriacea counts (TEBC), meanwhile, total yeast count (TYC) was increased. It can be mentioned that adding nature bioactive mixture (LOG) in drinking water of Rex rabbits occurred an improving in their growth Badr et al., Nutritional impact of inclusions natural bioactive mixture in drinking water Bioscience Research, 2019 volume 16(2):1808-1823 1809 performance, decreasing mortality rate, decreasing both blood total cholesterol and blood triglyceride. Also, it had no adverse effect on carcass characteristics or ceacum parameters and improves the microbial activity. The best levels of adding from LOG was 20 ml LOG/ liter dinking water