Microsatellite records for volume 8, issue 1

Full text

UNCORRECTED PROOF
MICROSATELLITE RECORDS
1
2Microsatellite records for volume 8, issue 1
3Maria Cristina Arias •Ste
´phane Aulagnier •Erin F. Baerwald •Robert M. R. Barclay •
4Jacqueline Silva Batista •Rochelle R. Beasley •Rodrigo A. Bezerra •
5Fre
´de
´ric Blanc •Eli S. Bridge •Maria Teresa Cabria •Lı
´via Izabela Caputo •
6Jie Chen •Jian-Hua Chen •Zhi Chen •Ching-Hung Chen •Pengfei Chu •
7Andrea Contina •Fausto Moreira da Silva Carmo •Leiliane Campos de Carvalho •
8Priscila Maria de Freitas Viana •Jeferson Luis Vasconcelos de Mace
ˆdo •
9Gabriel de Menezes Yazbeck •Ze
´lia de Oliveira Teixeira •Nata
´lia De Souza Araujo •
10 Gabriel Dequigiovanni •Shaoxiong Ding •Priscila Karla Ferreira Dos Santos •
11 Holly B. Ernest •Yan-Wei Feng •Kyara Martins Formiga •Christine Fournier-
12 Chambrillon •Elaine Franc¸oso •Yaru Fu •Roderick B. Gagne •Fernanda A. Gaiotto •
13 Tian-xiang Gao •Hongzi Ge •Taiming Ge •Franc¸ois Gillet •Yun Gong •
14 Hong-mei Gong •Qiqiang Guo •Liang Guo •Liang Guo •Ryan J. Harrigan •
15 Libo He •Carrie J. House •Chung- Der Hsiao •Rong Huang •Arati Iyengar •
16 Xiayun Jiang •Hai-Lin Jiang •Tania A. Johnson •Kenneth L. Jones •
17 Paulo Yoshio Kageyama •Jeffrey F. Kelly •Lydia Lam •Stacey L. Lance •
18 Hong Li •Cheng Li •Fugui Li •Huie Li •Shaoke Li •Yongming Li •
19 Mingming Li •Mingming Li •Fuhua Li •Wei-guo Li •Jitao Li •Jian Li •
20 Xinru Li •Kai Liang •Yaosi Liang •Fuchu Liao •Lanjie Liao •Haoran Lin •
21 Haoran Lin •Haoran Lin •Qiang Lin •Hong Liu •Honggao Liu •Lu Liu •
22 Xiang-Quan Liu •Ping Liu •Chen Liu •Xin Liu •Maria Teresa Gomes Lopes •
23 Danqi Lu •Ming-Bao Luan •Wei Luo •Zining Meng •Zining Meng •
24 Johan R. Michaux •Me
´lanie Ne
´moz •John P. Pollinger •Ye Qiao •
25 Hongyue Qu •Santiago Linorio Ferreyra Ramos •Stephne
´Le Roux •
26 Leandro Rodrigues Santiago •Xiaoyan Shao •Kang-Ning Shen •Heding Shen •
27 Hao Shen •Thomas B. Smith •Dang-yu Song •Eric Sourp •Yiwen Sun •
28 Huiyuan Tang •Qing Tang •Yiqin Tong •Marion van den Einden •
29 Moniek J. C. van Hoppe •Elizabeth Ann Veasey •Corinne Vial-Novella •
30 Li Wan •Xiaojun Wang •Yiming Wang •Yu Wang •Yaping Wang •
31 Ruoran Wang •Xin Wang •Rifang Wang •Quanchao Wang •Guo-dong Wang •
32 Jiajia Wang •Dongfeng Wang •Peipei Wang •Xiu-Mei Wei •Yanfei Wen •
33 Haishen Wen •Xue-Ping Wu •Xin Wu •Jianhai Xiang •Zhenzhen Xie •
34 Zhanning Xu •Pao Xu •Zhi Yang •Sen Yang •Sen Yang •Cece Yin •
35 Shaowu Yin •Shaowu Yin •Yang Yu •Xiping Yuan •Gen Hua Yue •
36 Gong-Gu Zang •Rafael Zardoya •Li-hui Zhang •Xiaojun Zhang •
37 Yong Zhang •Guosong Zhang •Yan- di Zhang •Hongye Zhang •Guosong Zhang •
38 Jie Zhao •Cheng Zhao •Fei Zhu •Hongchai Zhu •Shilong Zhu •Shuming Zou
39
40 Springer Science+Business Media Dordrecht 2016
41 42
A1 Electronic supplementary material The online version of this
A2 article (doi:10.1007/s12686-016-0522-2) contains supplementary
A3 material, which is available to authorized users.
A4
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44
Conservation Genet Resour
DOI 10.1007/s12686-016-0522-2

UNCORRECTED PROOF
43 44
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Synechogobius hasta S-7170
a
F:GGCTTTGCGATTTATCCTCA
R:CAGTGTACACAAGGGGGCTT
(AC)6) 178–288 32 16 0.594 (0.799)
Synechogobius hasta S-9662
a
F:CACCGTCCTTTTAGATCCCA
R:TGGAACTCAGGCCATAAAGG
(AC)7 248–256 32 5 0.656 (0.733)
Synechogobius hasta S-12224
a
F:TCCTCCCTGAGATTCCTCAA
R:TACCAAAAAGATGCACTGCG
(AT)6 180–188 32 5 0.594 (0.656)
Synechogobius hasta S-12344
a
F:TGATTGAGCCTTGATTTAGCG
R:GAGGCAAAGAAAGCCAAGTC
(AC)7 282–300 32 9 0.750 (0.818)
Synechogobius hasta S-9829
a
F:GCAACATCGATAGAAAGTGCC
R:CGCATAACAAAACTGATCCAAA
(AAT)6 246–263 32 10 0.625 (0.812)
Synechogobius hasta S-12421
a
F:TTCCGCTTCACTCACACTTG
R:AGTTTTAGCGCCATCTACCG
(TG)6 256–264 32 4 0.594 (0.477)
Synechogobius hasta S-14128
a
F:CATTTTCACCCGGTTCAGTT
R:GGAGACTCTGGGTCTGAACG
(TCC)5 186–216 32 11 0.750 (0.847)
Synechogobius hasta S-8301
a
F:ATATGCTGGAGGTGCCTCTG
R:AAGGGAAATCAGGGCTTCAT
(GTG)5 254–270 32 5 0.500 (0.486)
Synechogobius hasta S-10502
a
F:TTTGAGACAAATATGGACGCA
R:CAACACAAAAAGGAATGCGA
(AAC)5 146–155 32 5 0.125 (0.120)
Synechogobius hasta S-7130
a
F:TTTCCATTTCGAAGGGTGAG
R:GGATTTCATCAAGCCTCCAA
(TG)6 112–149 32 11 0.688 (0.785)
Synechogobius hasta S-14289
a
F:CTCTTGAGTCGTGTTGGTGG
R:CCCACACTTTGTCCGTGTAA
(CT)10 255–282 32 11 0.125* (0.841)
Synechogobius hasta S-15465
a
F:CGTGACCAATGTGTTGGAAG
R:TCAAGAACCCGGAGATCAAC
(GCT)5 205–278 32 19 0.000 (0.943)
Synechogobius hasta S-16908
a
F:AAACCAAACACGTGACCACA
R:AAAGCAGGACTTGGCAGAAG
(TC)8 148–172 32 15 0.656 (0.892)
Synechogobius hasta S-3285
a
F:GGATGCAGAGAGACAGGAGG
R:TCAACGCTCTTTCCAACTCA
(GA)7 206–217 32 5 0.063 (0.179)
Synechogobius hasta S-10067
a
F:TAGACGGAGCGCTTTACCTG
R:CTTTCACCGCAGATTCCAGT
(CCG)6 108–146 32 14 0.219 (0.745)
Synechogobius hasta S-10501
a
F:CTGCTGGCTCTGCTGTAGTG
R:ATTCGCTGCTGAAAGGAGAA
(GTT)5 198–232 32 8 0.156* (0.582)
Synechogobius hasta S-13590
a
F:GGACTCTGAAAGCTTGCCTG
R:GTCACGGTCTGGGTCTGAAT
(GAA)5 194–203 32 3 0.438* (0.657)
Synechogobius hasta S-13930
a
F:GTCACGGTCTGGGTCTGAAT
R:CACTCCAATATGCATGGCAC
(ACAT)5 203–365 32 7 0.000* (0.387)
Synechogobius hasta S-9028(1)
a
F:TCACCAGACATGACACAGATCA
R:GGGATGATGGGACCCTATTT
(TAT)6 200–277 32 22 0.000 (0.952)
Synechogobius hasta S-13120
a
F:TTGTCCCTCAACATCTTCCC
R:CCCAATCCCAAAAACACAAC
(CT)10 188–232 32 15 0.406 (0.903)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
45 46
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Synechogobius hasta S-11280
a
F:GGAGGACACTGATTCCCTCA
R:TATCGACGTGCCATCACATT
(AC)6 211–268 32 9 0.250* (0.797)
Synechogobius hasta S-13267
a
F:GTGGTTGGTGAACTCCGTCT
R:AGGACGTTGGAGAGTGTTGG
(TC)8 211–266 32 10 0.219 (0.663)
Synechogobius hasta S-9028(2)
a
F:AAATAGGGTCCCATCATCCC
R:TCTTGGCACAAAATGCTCTG
(CA)7 206–232 32 13 0.938 (0.826)
Synechogobius hasta S-4092
a
F:AAGCAGCCTTTGAAGACCAA
R:TTCTTTGCCCTCTGCTTCAT
(CGA)6 282–302 32 16 0.469 (0.86)
Synechogobius hasta S-15094
a
F:TGAACATATACATGCGGCCT
R:GCAGTGTAATGTTTTGGGGG
(AC)9 212–244 32 16 0.938 (0.908)
Synechogobius hasta S-9726
a
F:GGCGTTCTTCTGGACTTCTG
R:ATCCCCTCTTCTCTTGCCAT
(AG)6 109–137 32 23 0.938 (0.937)
Synechogobius hasta S-9329
a
F:AAGCTCCACCCCGAAACTAT
R:ATTCCAGGGAAGGTTTCCAC
(ATT)5 237–288 32 15 0.250 (0.485)
Synechogobius hasta S-1553
a
F:TTCTCCTACTGCTCCCCAGA
R:ACCCAACAGTTTTGACTCGG
(AC)8 237–271 32 22 0.781 (0.898)
Synechogobius hasta S-14078
a
F:TGTTGTTTGCCTGTGGGATA
R:TCATCCAGATCTGATCCACG
(GCC)5 162–254 32 22 0.750 (0.917)
Synechogobius hasta S-1515
a
F:TCTCTGTGCCAAAACAGTCG
R:CCCCGGCTCAGTATCAGTTA
(AGG)5 274–313 32 13 0.781 (0.898)
Synechogobius hasta S-11159
a
F:AGATAGACATTTCCGCCCCT
R:GTGAGTGTGGAGAAGGCACA
(CT)9 105–130 32 21 0.781 (0.935)
Synechogobius hasta S-13449
a
F:TGTGGTCGCTCCTTTCTTCT
R:CGTCAGACTTCCGGACTTTC
(AGAA)5 229–286 32 24 0.000 (0.962)
Synechogobius hasta S-12331
a
F:TTGCCGACAATGTTGGAGTA
R:TCCACAACAACGCAGTAAGG
(TG)7 228–281 32 20 0.063 (0.950)
Synechogobius hasta S-5685
a
F:TTCGTTGTTTCCGATGTAACTG
R:TTTGTTTTGGGATCTGATTTGA
(AC)6 162–180 32 8 0.125 (0.338)
Synechogobius hasta S-15402
a
F:ACTCGTCAGGCGTTTCAGAG
R:TTCCCGTTTTTCATCTGGAG
(GAG)5 247–289 32 8 0.000* (0.720)
Synechogobius hasta S-12326
a
F:ACAGTAACAGCAGCGTCCCT
R:ATGTTTGTGCTCAGTGCAGG
(CTG)5 176–293 32 23 0.563 (0.930)
Synechogobius hasta S-1910
a
F:GTTGACGCTCCCTTTGTAGC
R:AGTTGACGTTCCACCGAAAC
(AG)8 230–281 32 22 0.281 (0.653)
Synechogobius hasta S-12897
a
F:GCTGAGAAATCAAGGAGGGA
R:ACAGAGCTGAAGAAAGCCCA
(TG)7 138–140 32 2 0.031* (0.198)
Synechogobius hasta S-17524
a
F:TTTTGCGTAGAATCACGCTG
R:GCCTGAGAAACTGCCTATGG
(AAAG)5 270–328 32 13 0.500 (0.818)
Synechogobius hasta S-7829
a
F:TTCATGCACGTAAAGCCAAA
R:GGCATTTTAGGGGAAAGAGG
(AT)6 247–325 32 17 0.250 (0.861)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
47 48
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Synechogobius hasta S-11814
a
F:TAGTATTGGCGGGCATTAGG
R:TGGACCTGAACTTGAGGGAA
(GT)6 242–263 32 20 0.750 (0.918)
Synechogobius hasta S-9043
a
F:TAGCCGTTTTGTGTCATGGA
R:GACACGTGGCCAAATAACCT
(AC)7 176–260 32 18 0.719 (0.893)
Platevindex mortoni PM1
b
F:CAACAAAGGTGGATAAAGAATGG
R: GGTCTACAGAAGGAGGGATGG
(GAAC)6 127–154 36 4 0.5278 (0.5372)
Platevindex mortoni PM5
b
F:ATCCTGGTGTTATTATTTTG
R:TTATGACTTCCACTCCCCTA
(TTAA)5 173–186 36 2 0.1667 (0.1549)
Platevindex mortoni PM6
b
F:TCCTCCTATGGAACTTCACTG
R:ATCTGCTTTGTCTGCCTCAT
(TCAA)10 171–184 36 2 0.5000 (0.5055)
Platevindex mortoni PM7
b
F:AGCGACGATAAACACTGGAA
R:CGTGCTGCTCTATGAATGAAC
(TCAG)6 294–343 36 2 0.1944 (0.2625)
Platevindex mortoni PM9
b
F:ATCTGTTGCCGTATGGTGCTG
R:TCTGGATGGGTTGGCTTTGT
(GTCA)5 162–204 36 2 0.0556 (0.0548)
Platevindex mortoni PM13
b
F:CAAACGAAACGCAGAATAAG
R:AACTGACCTGGTGTAAATGA
(AAAC)5 250–260 36 2 0.1944 (0.1780)
Platevindex mortoni PM14
b
F:TCAAAGAAAGAGCCGAGATT
R:AGGGCAGGTAACTGAACTAA
(ATT)6 285–320 36 3 0.3333 (0.2895)
Platevindex mortoni PM15
b
F:GTAACCTACCAAAGAGGACCAG
R:TCAGCACTAGCGAATCAACC
(TGT)6 131–139 36 2 0.1111 (0.1064)
Platevindex mortoni PM16
b
F:TAACATCATCTTCACCAGCATCA
R:TAACATCATCTTCACCAGCATCA
(ACC)7 565–625 36 2 0.1389 (0.1311)
Platevindex mortoni PM23
b
F:AAGCCTCCTCGGAAAGTGCC
R:CGTGAAAGTTGTTGCGGTGGTAG
(AGG)7 325–335 36 2 0.1944 (0.2218)
Platevindex mortoni PM25
b
F:ACGCAATGGGTAGGACTCGC
R:GAAGACAAGAATGGCAGCAACG
(CGA)6 246–261 36 2 0.1389 (0.1311)
Platevindex mortoni PM27
b
F:GTAAACAGCCAACAGGAGCAA
R:AACACGCCACTGGACACGAA
(TTC)6 225–325 36 3 0.0278 (0.0278)
Platevindex mortoni PM28
b
F:AACTTATCACCCTCGCCTCA
R:CAGAAAGCCAGCGTAACAGC
(TAT)6 129–150 36 4 0.2778 (0.2516)
Platevindex mortoni PM32
b
F:AGAAGCAAAGTCCTCGTTAG
R:CAGAACATCATCAAATACCG
(AAT)7 220–341 36 3 0.0556 (0.0552)
Platevindex mortoni PM41
b
F:GGGATTTCTCGCTCTTCATT
R:TTTCTGTTACTACACCCTTTG
(CT)10 279–400 36 3 0.4722 (0.3916)
Platevindex mortoni PM42
b
F:GGACCTAAGAGGCAAGAAACA
R:AGTAACCGTAAACACTCCATCAC
(GAA)6 246–300 36 2 0.0625 (0.0615)
Platevindex mortoni PM46
b
F:GTTGTTTGCTGTTATTGTTCGCTAT
R:CCTTCAGTTGTCTCCCGTCA
(TGT)6 90–110 36 5 0.8214* (0.8370)
Platevindex mortoni PM48
b
F:TGGTTCCCAGGCATTTCTCC
R:CAGTTGGTCCAGTCCATCATCC
(GTG)6 149–262 36 2 0.1250 (0.1190)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
49 50
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Platevindex mortoni PM55
b
F:GCGGCTCATTATCACAAACT
R:ATCACCTCTTCCCAACAATCT
(CAG)6 148–244 36 3 0.2813 (0.2941)
Platevindex mortoni PM56
b
F:TTTCATTTAGGGCTCCATTC
R:TTCCCAGTATTTCCTGTTCG
(AT)9 247–275 36 2 0.0313 (0.0313)
Platevindex mortoni PM57
b
F:TTCTTATCGCTATCTCACTT
R:AATGGAACAACTACAATCTG
(TC)9 200–205 36 2 0.4706 (0.5134)
Platevindex mortoni PM60
b
F:TTTTAGTATTTTCAGCAGGGGTTT
R:CAGTGGACGATTTGGGCATT
(AG)9 248–260 36 2 0.9688* (0.5074)
Onychostoma sima OS-SSR1
c
F:AGTCTACATCCAAGTAAATGCC
R:GCCAAATAACCATTATTAATTTTA
(AATG)8 128–140 40 3 0.200* (0.541)
Onychostoma sima OS-SSR2
c
F:TTCTTAATTTGTTTTATCATGAATG
R:AATAACATTTCTTTTAGATTGCTAC
(AATG)9 113–161 40 6 0.875 (0.763)
Onychostoma sima OS-SSR3
c
F:TTCCTGACATGGTTTTCTTCTC
R:CCTTGCCATTGTTGCCT
(GGT)5 123–159 40 6 0.600 (0.562)
Onychostoma sima OS-SSR4
c
F:AAAAGTGTGTGTGTGAGAGTGAG
R:CAACAGTCCCCAATGAAATG
(TGAG)7 99–123 40 6 0.650 (0.645)
Onychostoma sima OS-SSR5
c
F:GAATGTCTGAAATTATTACTTTTTA
R:TGACAGCACTAGTAAGTATGTATTC
(ACTG)9 125–149 40 8 0.553 (0.742)
Onychostoma sima OS-SSR6
c
F:ATGCACATGGAGAAAATCTATG
R:ATAGACAAACATACAGACAGATAG
(ATCT)13 105–121 40 5 0.800 (0.714)
Onychostoma sima OS-SSR7
c
F:AATAATGACATAATGATTTGATAATG
R:GGAGATAATGGTGTTCACTTTT
(AGAT)8 167–199 40 7 0.923 (0.816)
Onychostoma sima OS-SSR8
c
F:TACGATAAAGGCTGCTGTATAG
R:CTTCTAGTATTGCATGTGGTTG
(TTTC)12 250–298 40 11 0.925 (0.885)
Onychostoma sima OS-SSR9
c
F:TCATTGGCTGATATGTGACC
R:ATTCTTATTTGCGAAAAGGA
(TTTC)9 186–258 40 9 0.850 (0.829)
Onychostoma sima OS-SSR10
c
F:GTCTTCATTTTGTCATTTTGGTA
R:ATGATTGACAGTTTTCAGCCT
(TTTC)11 260–300 40 8 0.900 (0.826)
Onychostoma sima OS-SSR11
c
F:CTAGGCAGTGGCTAGAGTATTT
R:ATTCTACTTTTGACTGGTAGAAGAG
(AAAG)12 162–214 40 8 0.650 (0.788)
Onychostoma sima OS-SSR12
c
F:AAGGCGCTATAGAAAATACAGAT
R:TTAAATTACATACAGGCCTAACAA
(AAAT)6 196–234 40 6 0.825 (0.787)
Onychostoma sima OS-SSR13
c
F:CATACTGTTCGTACAGTATTGGTGT
R:CTTGTTGTTTTAAGGATGTTTAGAT
(AAAT)11 249–277 40 7 0.692 (0.699)
Onychostoma sima OS-SSR14
c
F:TTAAATGAGTTTTCTTTGCTTTAG
R:CCTCCATGTTTCTGTTGATGTT
(AAG)8 218–239 40 8 0.944 (0.838)
Onychostoma sima OS-SSR15
c
F:TACTCCTGCAGAAAGTGAGTCC
R:ATGTTTTAATAGTTTTAGTTCGTGA
(TTA)11 142–172 40 8 0.906 (0.821)
Onychostoma sima OS-SSR16
c
F:ATCGTATTCTTTCAAATACAATTC
R:AAGAGTGCATCGCATTACTAA
(AAT)9 212–224 40 4 0.710 (0.700)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
51 52
Species Locus Primer sequence (50–30) Repeat motif Size range (bp) Sample size (N) Number of alleles (N
A
)H
o
(H
e
)
Onychostoma sima OS-SSR17
c
F:AAAGATTCTAAAACCTATAACCCTG
R:AAATAAACAACATTTTCTGCACTC
(AAT)10 200–227 40 8 0.795 (0.715)
Onychostoma sima OS-SSR18
c
F:TACTCCTTAAATTCTCCTCACCT
R:TCAAATAAGTATTGGACCCCTA
(TTA)11 172–190 40 7 1.000 (0.837)
Onychostoma sima OS-SSR19
c
F:CATGTTAAAGCCGTAAATTGTG
R:TTAAACAGTGTTAGGTTGTGCC
(GATT)9 170–202 40 9 0.667 (0.734)
Onychostoma sima OS-SSR20
c
F:CAACTGAGGCAACTGTTGAGTA
R:CCTGAATGAATCTTATTGAGCTG
(CATT)5 188–208 40 5 0.750 (0.694)
Onychostoma sima OS-SSR21
c
F:ATAGTTAAATACAGTAGCTATGGCT
R:TACATTCATGTACATCTATCTATCAC
(AGAT)18 208–256 40 11 0.725 (0.848)
Onychostoma sima OS-SSR22
c
F:AATTGTACAGATCAGTAGCAGCA
R:GATTTGAACATAATGAAGGAGAA
(ATCT)16 264–324 40 11 0.750 (0.799)
Onychostoma sima OS-SSR23
c
F:ATTTATCACGCTTAACCCATACC
R:CCAAATACCCCCTTCAGAC
(AGAT)14 226–274 40 12 0.875 (0.856)
Onychostoma sima OS-SSR24
c
F:CAATTATACTACTCTCCAAAAGTGC
R:GATAGCTAAATGACTATTAAACTTG
(ATCT)17 163–223 40 11 0.900 (0.882)
Onychostoma sima OS-SSR25
c
F:CCTGGTGAGAGAAAATGAGG
R:CAAGCTAAGTAATTGCAGAATGTA
(ACAT)7 108–128 40 4 0.400 (0.393)
Onychostoma sima OS-SSR26
c
F:TCTGGTAAGAGATAACTGGGTGT
R:CCTACTTCGACTACTTCAGCTAC
(GTA)10 111–129 40 4 0.525 (0.499)
Onychostoma sima OS-SSR27
c
F:TTTAGAGGGTGCCCATAGTAAC
R:ATTTCGGTGGTGTCAAGC
(AGC)8 255–264 40 4 0.605 (0.627)
Euterpe precatoria Epr01
d
F: \HEX[AGAGCTATGATGTGCGCTACC
R:GTGTCTTGGCCTTTCTCTGC
(CT)13 (GT)11 158–170 34 4 1.00* (0.55)
Euterpe precatoria Epr02
d
F: \FAM[CTTCTGCCATGCTCCTCATAG
R:CACAAACCTCCCTCCTAAGCTA
(TC)18 278–306 34 6 0.53* (0.58)
Euterpe precatoria Epr03
d
F: \NED[TCTCCATCTCCAACCTCCTCT
R:CTGCTCTATCCCATTCATCCC
(TG)16 AGCGAGTGAGT (GA)16 279–317 34 7 0.59* (0.68)
Euterpe precatoria Epr04
d
F: \HEX[CTCCAACCTACAAGAACAGCC
R:AGAAGCAGCAGCAACAGGTAG
(AG)19 257–265 34 4 0.38 (0.51)
Euterpe precatoria Epr05
d
F: \HEX[CTCTTGGATGGTGTCCTCTTCT
R:ACCAGACCCTCCTTTCCACTA
(CT)15 147–159 34 6 0.94* (0.76)
Euterpe precatoria Epr06
d
F: \FAM[CAGGGAAAACCTAGAACAGGC
R:5CCAGAGTATGGGTGATGCACT
(GA)19 226–262 34 8 0.41* (0.60)
Euterpe precatoria Epr08
d
F: \NED[GCCAGCAATACCAGGAGGTTA
R:GTGGGTGTGACTTTGTCGTG
(TC)15 203–221 34 7 0.68 (0.60)
Euterpe precatoria Epr13
d
F: \HEX[ATGGAAGTGGATCTGGCAAC
R:GTCAAGTAGGGAGTCCAGCAA
(AC)21 240–262 34 5 0.47* (0.68)
Euterpe precatoria Epr14
d
F: \NED[GCACATCTAGCAGCGTTCTCT
R:CAGACCGAACTGCCAAAAGT
(GA)22 238–262 34 5 0.15* (0.42)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
53 54
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Euterpe precatoria Epr15
d
F: \NED[GCCTTGCTGCTAATCTTGTG
R:TGAATATACGCCATCCCC
(TG)10 (AG)9 129–151 34 5 0.97* (0.59)
Euterpe precatoria Epr18
d
F: \FAM[CATGACAGTTTCGGAGCCTT
R:ATACCAGCCCATATTGCACC
(CT)8 212–240 34 8 0.59* (0.51)
Euterpe precatoria Epr19
d
F: \FAM[GAGAGTTTGGTTGTCGAGCTG
R:AAGCCTGATCTGTTGCGAGT
(TG)6 143–177 34 8 0.47* (0.74)
Euterpe precatoria Epr21
d
F: \FAM[CACTCTCCCCAAAGGAAGAAG
R:ATACTGGAAAAGCAGACCCC
(GT)18 224–234 34 3 0.29 (0.30)
Euterpe precatoria Epr22
d
F: \FAM[CTTTGGATGCTGGAAGGAAC
R:GCATCATACCATCAAGAGCG
(GA)17 143–147 34 3 1.00* (0.51)
Euterpe precatoria Epr25
d
F: \HEX[GGAGAACCTATTTCAGCCTGC
R:GAGGTTTGTGTTCACTCCTGG
(TC)6 224–276 34 11 0.44* (0.58)
Euterpe precatoria Epr26
d
F: \NED[AGGGGACAGAAATGGCTACA
R:CAATGCAACAGCAGAACCAG
(AC)14 147–169 34 12 0.91 (0.83)
Euterpe precatoria Epr27
d
F: \HEX[GTATGCACATTAGGGCTTCGC
R:ATGCGTGCGTGTTTTGGG
(CT)7 234–262 34 5 0.47 (0.39)
Euterpe precatoria Epr30
d
F: \NED[CGCTTTTCTATCCATCCCC
R:AGCAGAGAAGGGAAACCTGAG
(TC)6 (CA)12 349–367 34 6 0.35 (0.36)
Euterpe precatoria Epr31
d
F: \HEX[CTGTTTCCAACCTACTACTCCC
R:AAGCCTGCTAACCTGATAGGC
(CGTG)5 (TA)6 316–340 34 4 0.94* (0.63)
Euterpe precatoria Epr32
d
F: \NED[CTCTCTCTTTCTCTATGAGGGG
R:AGGGCAGAATGTCCACTAGC
(TTC)6 239–257 34 5 0.44 (0.43)
Euterpe precatoria Epr33
d
F: \HEX[GGCAAGGCTGTGAGAAAGTT
R:GGAGGGCCACTAGCTCTACTC
(GA)9 154–180 34 10 0.79 (0.80)
Euterpe precatoria Epr35
d
F: \NED[GTGTCGTGTAGGAAGCAATGG
R:GATCCGAGCCCATTTTGG
(AG)9 224–242 34 8 0.88* (0.80)
Euterpe precatoria Epr36
d
F: \FAM[CCTCTGGAACCTGTCAACAAG
R:CTTTCCTCCTCTCCGCTAGAA
(AG)19 156–180 34 7 0.47* (0.47)
Coilia nasus Cnasus3
e
F:CAGAACGCCTCCTCTCTCAG
R:GACGACCACCTACCTCCCTC
GAG(3*7) 152 36 5 0.4225* (0.7438)
Coilia nasus Cnasus4
e
F:AGGACAACATCAGCTTCTCAAAT
R:AGTGGTGGCGGTAGTATCAGTAG
ACC(3*7) 149 36 6 0.4225* (0.8014)
Coilia nasus Cnasus5
e
F:GAAATGGAGGCTGCTGATAAAG
R:CCTGTTCTGTTTGTTTTTCCTTG
GAG(3*7) 114 36 3 0.2340* (0.5216)
Coilia nasus Cnasus7
e
F:ACCACAGTAGCCAAGAACGG
R:GTAGCTCAAGACAGGCAGACAAG
CCA(3*8) 153 36 2 0.2766 (0.4139)
Coilia nasus Cnasus8
e
F:GGAAACAGACAAAACAGCAAAAC
R:AGCCATGAACTGCAGAAAATAGA
TTG(3*8) 157 36 3 0.5532 (0.6577)
Coilia nasus Cnasus9
e
F:GTCACTTACATTTTACGGGGGA
R:GTCCTCTGATTGTGGATGTTCTC
ATA(3*7) 140 36 4 0.4681* (0.6710)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
55 56
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Coilia nasus Cnasus12
e
F:TCTTCTGGAACAACCTACGACAT
R:AAGGTGCTGGAGGAGATGG
CTG(3*7) 152 36 2 0.4043 (0.3507)
Coilia nasus Cnasus22
e
F:GGTTTTGTCATTTTCCATATTGC
R:CCACACCCTACTATGACCTTTGA
GGT(3*7) 94 36 3 0.4681 (0.5813)
Coilia nasus Cnasus25
e
F:AAAAGGCAGGACTGGACAAAT
R:ACCGACAGGTGAAACTTTGG
CAG(3*7) 83 36 2 0.3404 (0.4997)
Coilia nasus Cnasus26
e
F:ATGAACAATGACAACAATCACCA
R:TGCCTTTACTGAGGACTTTGTGT
AAC(3*7) 133 36 2 0.4468 (0.4139)
Coilia nasus Cnasus27
e
F:CTTGTAGTTGGTTAGGGCAGAAA
R:GGACGTTTAAGTAGCATGGAATG
TTG(3*8) 117 36 5 0.5957* (0.7495)
Coilia nasus Cnasus28
e
F:CAAAAAGATGCTCTGAGGAGAGA
R:GCAGACACAGACACTTGGGATAG
TCCG(4*6) 117 36 3 0.5957 (0.4850)
Coilia nasus Cnasus29
e
F:ATCTCCATCCTTCTTTCCTGTGT
R:GACAGAGTGAACGAATGAGCTTT
CTC(3*7) 139 36 3 0.5106 (0.5509)
Coilia nasus Cnasus34
e
F:TGGTGTAGGTGTGGGACTTGT
R:CACCACATCAGCCAGTCCT
GTG(3*7) 100 36 3 0.5106* (0.6351)
Coilia nasus Cnasus35
e
F:CTGTCTGACCCCCTAGCTCTC
R:TCTTTCTTTTCTGCTGAAGTGGT
AGA(3*7) 143 36 2 0.3191 (0.5045)
Coilia nasus Cnasus36
e
F:GAGAACAGGATGTGGAGGAGAT
R:CTGTGCAAGTCAATGTGACAGAC
CAG(3*7) 118 36 3 0.6596 (0.5868)
Coilia nasus Cnasus37
e
F:GAGTGCTACGACTCACCTACCAT
R:TAGCAAAGGAGACCGACTGTTAG
TA(2*11) 159 36 6 0.4468* (0.7625)
Coilia nasus Cnasus39
e
F:AGAGTGACCTGGATGCACTGAT
R:GGGCGACGAGTGGTGAAG
ACC(3*7) 118 36 2 0.5745 (0.4605)
Coilia nasus Cnasus41
e
F:CTCGTTGATCTGGATGTTGATGT
R:ACCCTCCCCGAGACACTACT
GTG(3*7) 157 36 3 0.8750 (0.5629)
Coilia nasus Cnasus43
e
F:TGTAGAATGGATTCCCAGCATAC
R:TATTAAAAATAGACACGGCGGAC
CTGC(4*6) 136 36 2 0.2083 (0.4034)
Coilia nasus Cnasus44
e
F:CTCCTCAGTGCTCAGTGGTAACT
R:CTTTACAACACACATGCTTACGG
CA(2*6) 80 36 2 0.4167 (0.4965)
Coilia nasus Cnasus46
e
F:ACCAATGGTCATTCAGACAGACT
R:GTGAGTTGCATGTGTGTTCCTTA
GAA(3*7) 137 36 2 0.9167 (0.5071)
Coilia nasus Cnasus49
e
F:CAGATTAAAGTTCATGAGGCTGG
R:GTATTCGCTGTTTGTGCTTCTTT
CAT(3*7) 136 36 3 0.2500 (0.6090)
Coilia nasus Cnasus51
e
F:ACTACACCTAGCATGGTCCAAAG
R:GCTCTGCACAGCTTCATTTTATC
GAA(3*7) 144 36 2 0.8750 (0.5027)
Coilia nasus Cnasus53
e
F:GAACATCTTCTGGAAGTCTGTGG
R:CCTTCTGCTATGAGAACGAGGT
CCT(3*7) 143 36 4 0.4167 (0.4911)
Coilia nasus Cnasus54
e
F:CGCATTATGATCCAGTTTAAGGT
R:AAAAAGGGCAGAACATTTTAACC
TTTA(4*5) 129 36 3 0.7500 (0.5470)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
57 58
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Coilia nasus Cnasus55
e
F:AGTTTTTCAGTGCGTTTGTAACC
R:GAGGCATCGATAAGAACCTGTCT
CAT(3*7) 145 36 2 0.5833 (0.5071)
Coilia nasus Cnasus57
e
F:GCTCCTACAGTTTCTTACAAAGGC
R:TTTTCATAGCACTCACAATTTCG
ATA(3*7) 160 36 3 0.7083 (0.5186)
Coilia nasus Cnasus58
e
F:GACTGTGCCTGCTGAAGATACTC
R:GGTCTTCAGGTGGTAGACAGGT
CCA(3*7) 103 36 2 0.7083 (0.5027)
Coilia nasus Cnasus60
e
F:TTTATGAACAGACCGAAGCTTGT
R:ATGACTACTGGTGAGCAAATCCT
AAG(3*7) 141 36 2 0.7917 (0.4885)
Coilia nasus Cnasus62
e
F:GCCATAGTACCTGTCCTTTCTGA
R:GGGTAGTGTTGGCTTTACACATC
AAT(3*7) 109 36 3 0.1250 (0.1215)
Coilia nasus Cnasus64
e
F:GGAAACCCTGGAACTACTTCACT
R:TGACATCAGTCCTGATATTGGTG
AAT(3*7) 143 36 3 0.8750 (0.5895)
Coilia nasus Cnasus65
e
F:GAATGGACACGTCTGTTTTTACC
R:GTTATGGCAAGGTGAAGAATTTG
TAT(3*7) 148 36 2 0.2083 (0.4388)
Coilia nasus Cnasus66
e
F:ATATATCTGCTTTCAAGGGGGAG
R:TAGTTGGTTTTGTCGTTTTCCAT
CAC(3*7) 80 36 2 0.7083 (0.5027)
Coilia nasus Cnasus68
e
F:GCTTGTCCAATCAGACACCTC
R:GAGGATGGAGCTGATCTTGC
CTC(3*7) 140 36 2 0.3750 (0.3112)
Coilia nasus Cnasus69
e
F:CAGGCCAGAGAGAACAGAGG
R:TTTAGAAATGCTGAGTGGACACC
CTG(3*7) 148 36 2 0.0833 (0.0816)
Chaetomys subspinosus Chae02
f
F:AGGCAATCTTATCTGAGCACTTG
R:TCAGAGCAAGGGTACGACAG
(AC)24 148–192 20 6 0.824 (0.656)
Chaetomys subspinosus Chae06
f
F:TGGCAGAGCACTTTTGGTTC
R:TGCAGCATTTCTTCTAACATTTGTG
(AC)13 82–112 20 6 0.368 (0.679)
Chaetomys subspinosus Chae07
f
F:CTCTCTGTGTGCATGTGCG
R:TGGTCGTATACCCTTATGGC
(GT)14 108–142 20 7 0.563 (0.829)
Chaetomys subspinosus Chae08
f
F:GTAGCTCAATGTGAAGCCCC
R:TACTCAGGCGTGCATCCTAC
(AC)22 134–162 20 6 0.895 (0.671)
Chaetomys subspinosus Chae10
f
F:GTGTGGAACAGCCTCACTTG
R:TTACACATGGGCCCCCTTC
(AC)11 108–158 20 6 0.4 (0.545)
Chaetomys subspinosus Chae13
f
F:ATGAGATGTTCCCAGAGGGC
R:CCACAGAGTTTCAAGGTCAGC
(AC)14 126–178 20 17 0.765 (0.947)
Chaetomys subspinosus Chae14
f
F:ATTGTGGACCTGCCAGCC
R:ACGCGAGACCGCCAATTC
(AC)12 126–142 20 8 1.000* (0.75)
Chaetomys subspinosus Chae15
f
F:TCAAGACAGGTGTGGTAGGC
R:TCTCCAGATTCCACTGCACG
(TG)12 104–152 20 8 0.364* (0.805)
Chaetomys subspinosus Chae18
f
F:CCATGGACTCAGCATGGTTTC
R:GAGTTTCTTCAGCAGGTGGC
(TG)16 126–164 20 3 0.9 (0.559)
Chaetomys subspinosus Chae28
f
F:CCTTGCTGTTGATTCCCGAC
R:AGTCCCCTCGAAATCCTCTC
(CA)12 144–164 20 8 0.563 (0.815)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
59 60
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Chaetomys subspinosus Chae30
f
F:AGCTGCATAAAGAGCAAGGG
R:GCCAAACAACTGTGGTCTCC
(AC)14 102–176 20 14 0.692 (0.932)
Chaetomys subspinosus Chae32
f
F:ACTACTGTCGCCCAGAACAG
R:GGCACATTTGTCCAAGGTCAG
(CA)13 150–178 20 2 0.765* (0.508)
Chaetomys subspinosus Chae39
f
F:ACTGAAGAAATTCATGACCACC
R:AAGACTGACACAACACACGC
(TG)14 82–116 20 10 0.9 (0.871)
Chaetomys subspinosus Chae45
f
F:GAGCAGAGCAAGTGTCCATC
R:CGCTGTCTCTTCTCTCAAGC
(CA)18 100–142 20 11 0.75 (0.869)
Chaetomys subspinosus Chae51
f
F:TGCGTAACTCCCTCTGTCTC
R:AATGGGCTGTTCTGGTAAGC
(CA)12 190–296 20 10 0.895 (0.836)
Chaetomys subspinosus Chae53
f
F:CACTGTGTAGTTCAGTCACTCC
R:TCTAAGCATGTGTGAGCCCC
(GT)12 100–122 20 7 0.143* (0.791)
Chaetomys subspinosus Chae58
f
F:TGGGGCTAAGGCTCAGTATG
R:TTCGTGAACCAAGGTACCGC
(AC)13 114–164 20 13 0.706 (0.875)
Chaetomys subspinosus Chae68
f
F:GGGAGCGTGGATTTTTCTGG
R:TCCGTCTTGTCTCATTCTCAAC
(GT)16 116–156 20 8 0.5 (0.804)
Chaetomys subspinosus Chae69
f
F:TGACCCTGGGAATCATAGCTG
R:ACACCACAGGTCTGTCTACG
(GT)13 86–110 20 8 1.000 (0.806)
Chaetomys subspinosus Chae78
f
F:GCCTCAGCCTTTGAGATTGC
R:TCAGATGCTCAGTTCAATACCC
(GT)20 102–152 20 8 0.714 (0.857)
Chaetomys subspinosus Chae80
f
F:CCTCTTTAGCCCACCCCTTC
R:CTAAGTGCATTTGCGTGTGC
(CA)12 102–138 20 5 0.571 (0.603)
Chaetomys subspinosus Chae85
f
F:TGGCTCACATTATTTCTCACCC
R:TTAGCCAGCTCTTTGTGTGC
(AC)16 100–120 20 7 0.947 (0.787)
Chaetomys subspinosus Chae88
f
F:CGCATGTGTGCCTGTGAG
R:AGTGAACAGTTTGCGATGCC
(GT)17 82–120 20 11 0.95 (0.865)
Chaetomys subspinosus Chae89
f
F:AGCACACAGTCGAGAAGAGG
R:ATGGCAAAGAGATGTGCGTG
(CA)11 134–160 20 5 0.444 (0.459)
Chaetomys subspinosus Chae95
f
F:GATCTGGCGTGAGTGGGAG
R:GTCACGGGTGAGTGTTTTCC
(CA)18 192–246 20 14 0.895 (0.903)
Chaetomys subspinosus Chae96
f
F:CGTCCTCCTTGAGGGTGTAG
R:TCACTGCCCACCTGTGATAC
(AC)11 180–190 20 7 0.273* (0.593)
Chaetomys subspinosus Chae98
f
F:CTCAGTACCTCCCTACGCAC
R:ACCTGGCTCCTTTCTTCATTTATTAG
(AC)11 84–114 20 5 0.25* (0.389)
Chaetomys subspinosus Chae99
f
F:TATGAGCAAGCGTGTGTGTG
R:CCAAACCAAAATAGGAGAAACAGC
(TG)13 82–98 20 4 0.65 (0.56)
Chaetomys subspinosus Chae101
f
F:TCTCTCTCGCACGATGCAC
R:ACCCTGCTATTGCTCTTCCC
(CA)17 112–156 20 14 0.95 (0.885)
Chaetomys subspinosus Chae102
f
F:GATTTGCTCTGTGCTCCCTG
R:ATCGTTAAGTAGATTCACACTGC
(CA)14 132–178 20 9 0.923 (0.871)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
61 62
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Spinibarbus caldwelli P4
g
F:TATTCGCATCAGAGCTGCAC
R:GCTCTTTCCAAGTGCCTCAC
(CA)
9
193–220 48 6 0.625 (0.763)
Spinibarbus caldwelli P18
g
F:TTCTTGTGTTCAGTGCTCCG
R:TCTAAGAAGGAGAGCAGCGG
(AC)
11
184–217 48 8 0.619 (0.767)
Spinibarbus caldwelli P23
g
F:TTTGTCTTTTTCAGGCCAGG
R:CCTGCCAACTTGTCCAGTGT
(TG)
11
214–257 48 9 0.708 (0.856)
Spinibarbus caldwelli P27
g
F:TGCTTCTGTTCATCAGCCAC
R:CCATTGCTGGTCTGGATCTT
(AC)
7
127–167 48 7 0.458 (0.761)
Spinibarbus caldwelli P28
g
F:GCGGTTCCGTGAGTGTTTAT
R:CAGAGCATCCTCTACCCCAG
(TC)
9
181–246 48 11 0.542 (0.875)
Spinibarbus caldwelli P35
g
F:TCCTCATCCTCATCCTCCAC
R:CAGCTTCAGCTCTCCGTTCT
(AG)
9
268–336 48 13 0.625 (0.900)
Spinibarbus caldwelli P41
g
F:ACAAGCCAAACCCCTTCTCT
R:CCCTGACCTTGATGTGCTTT
(GA)
9
221–257 48 8 0.583 (0.814)
Spinibarbus caldwelli P43
g
F:ACAAGGTAATGATGAGGCGG
R:ATGGCCATTTTCCCTTTACC
(GT)
10
157–196 48 6 0.250* (0.704)
Spinibarbus caldwelli P47
g
F:TGGATGTGGAGAGCACAGAG
R:AGGGAAAGGTGGCTAGGAAG
(CA)
9
225–275 48 11 0.458 (0.897)
Spinibarbus caldwelli P49
g
F:TATAGAACGGCAAGGCAAGG
R:TGTGTCTGCCGCTGAAATAG
(CA)
10
124–152 48 6 0.792 (0.805)
Spinibarbus caldwelli P52
g
F:CTTGCTGTGCAGCGTATTGT
R:AAATCAGCAGCATTCGGTCT
(TG)
9
267–359 48 11 0.833 (0.889)
Spinibarbus caldwelli P59
g
F:AGGACTTATCCTGGGCCACT
R:TGCCATGATTAACGAAACACA
(AC)
7
318–341 48 6 0.125 (0.762)
Spinibarbus caldwelli P64
g
F:TCCTGAGCAAGAAAAGCACA
R:TCCAACCTGCCTAAATCACTC
(CA)
11
217–259 48 9 0.472 (0.791)
Spinibarbus caldwelli P66
g
F:GTGACGCACGAAACTAAGCA
R:CATGCATTTATGCACAGCCT
(AC)
9
228–283 48 11 0.833 (0.876)
Spinibarbus caldwelli P67
g
F:CACTTTCCCTGCTGGTTTGT
R:TTGCACTGTAAACGCTGAGG
(CA)
9
250–298 48 11 0.292 (0.856)
Spinibarbus caldwelli P70
g
F:GAGGGACTGCAGCTTAAACG
R:TGCTGTTTTGGCTTCATCTG
(GT)
9
147–182 48 7 0.208 (0.821)
Spinibarbus caldwelli P81
g
F:GCCATGATATGCGTTCACAC
R:GCAAACATCACTTCATTTTGACA
(TG)
10
246–406 48 20 0.875 (0.942)
Spinibarbus caldwelli P93
g
F:TTTTTGGGTGTTGCTTGACA
R:GGGGGACAAATAAAAGCCAT
(GT)
9
184–230 48 9 0.667 (0.814)
Spinibarbus caldwelli P97
g
F:CCCTCAGAGAAGCATTAGAAGAA
R:TGTCTGCTTGCCTGCAATAC
(AG)
9
264–283 48 8 0.705 (0.897)
Spinibarbus caldwelli P99
g
F:GACATTGTTCTCTCCTGCCC
R:TCACCGCAATGCTAATTCAC
(GA)
10
96–131 48 7 0.674 (0.715)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
63 64
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Spinibarbus caldwelli P107
g
F:CACACCTCACACACCTCCTC
R:AGCACCAAAGCCAATGTGTT
(CA)
11
240–269 48 12 0.834 (0.910)
Taihangia rupestris
Taihangia ciliate
TrSSR1
h
F:ACATCATCGTAACCAAGACC
R:CCCTCAAAACCCAAAAGA
(AG)
10
230–234 20 1
3
0.000 (0.000)
0.471 (0.400)
Taihangia rupestris
Taihangia ciliata
TrSSR10
h
F:CACCACCATCGTCATCCTA
R:CCCACTGTCACCCTCAAAC
(AT)
10
(CT)
9
308–314 20 3
4
0.491 (0.350)
0.609 (0.500)
Taihangia rupestris
Taihangia ciliata
TrSSR13
h
F:TTGTCCAGTTGCCCACGAA
R:CAGAAAGGAAACGGCGAAG
(CT)
9
155–167 20 2
4
0.489 (0.650)
0.735 (0.750)
Taihangia rupestris
Taihangia ciliata
TrSSR14
h
F:CAAGCAACATTTACATACC
R:TTCTTTTCTTCCATTTCC
(AAAC)
8
234–242 20 2
2
0.495 (0.700)
0.480 (0.700)
Taihangia rupestris
Taihangia ciliata
TrSSR25
h
F:CGTCGGCTTTTCAATCTGG
R:AAACAACGCTTCTCCTCCCT
(GTT)
7
188–191 20 1
2
0.000 (0.000)
0.375 (0.200)
Taihangia rupestris
Taihangia ciliata
TrSSR31
h
F:GGGCGGTGCGTTGGATGTAG
R:GGTGGTGGTGACGGGGCTCT
(GGA)
7
194–200 20 1
3
0.000 (0.000)
0.579 (0.600)
Taihangia rupestris
Taihangia ciliata
TrSSR33
h
F:ATTTCTCGGGTCGGGTATCA
R:GCTTCCGTCATCAGCAGTCA
(TAA)
7
268–274 20 3
2
0.609 (0.550)
0.420 (0.200)
Taihangia rupestris
Taihangia ciliata
TrSSR35
h
F:TTCCTTCACCAATCGCTTTC
R:GCTTCCTCCTTCCTCTTCG
(AGC)
7
260–263 20 3
2
0.571 (0.250)
0.320 (0.400)
Taihangia rupestris
Taihangia ciliata
TrSSR37
h
F:GTGTTTCAGTTGACGATGTA
R:TTTCACTATGCCTTTTATGT
(TG)
10
302–306 20 2
3
0.489 (0.450)
0.465 (0.500)
Taihangia rupestris
Taihangia ciliata
TrSSR39
h
F:GGAGGAGGGCAGAAGGGTA
R:CAGGGAGATTGAACTTGACAGAC
(AAC)
7
309–318 20 2
4
0.455 (0.300)
0.726 (0.800)
Taihangia rupestris
Taihangia ciliata
TrSSR41
h
F:GACTACAGTGGTGGCTATGA
R:TCGTGCTTTTCTTTCTAATC
(AAC)
7
236–248 20 3
2
0.599 (0.400)
0.469 (0.250)
Taihangia rupestris
Taihangia ciliata
TrSSR44
h
F:GCTTCCTCCTTCCTCTTCG
R:TCCTTCACCAATCGCTTTC
(ACC)
8
280–286 20 2
3
0.469 (0.350)
0.559 (0.800)
Taihangia rupestris
Taihangia ciliata
TrSSR47
h
F:GAAGGGTAGCGGAGACAGAA
R:ATAGCCCGTTCCTTCGTTTT
(AG)
5
A(AG)
3
217–225 20 3
5
0.551 (0.700)
0.725 (0.500)
Taihangia rupestris
Taihangia ciliata
TrSSR50
h
F:TGGGTATCACCAGAGCG
R:GTGGAGGAGCACAAACG
(AGA)
8
344–353 20 3
3
0.480 (0.400)
0.601 (0.600)
Taihangia rupestris
Taihangia ciliata
TrSSR51
h
F:GAAGAAGGGAAAATAGAAAA
R:ACTCAAAGAGGGTGTTAGG
(CACTTC)
8
291–297 20 1
2
0.000 (0.000)
0.489 (0.650)
Taihangia rupestris
Taihangia ciliata
TrSSR52
h
F:CACAGCCAAATAGTAAAGC
R:AGACGAGGAGGAAGAAGAG
(CAATTT)
6
291–303 20 3
3
0.646 (0.600)
0.661 (0.900)
Taihangia rupestris
Taihangia ciliata
TrSSR55
h
F:TAATCTAATCCCAACCCTC
R:CATTCATACCGAAATAACCT
(GA)
8
335–341 20 4
4
0.536 (0.750)
0.479 (0.450)
Taihangia rupestris
Taihangia ciliata
TrSSR56
h
F:GGACTTTGCCTAAACCACCAT
R:CTGATTCCTCCCACTCTTGC
(TA)
8
271–273 20 2
2
0.375 (0.400)
0.499 (0.450)
Taihangia rupestris
Taihangia ciliata
TrSSR61
h
F:AAGCCCTGTTGGTCATTGTT
R:AAGCCCTCCATCTCCACTAT
(TAG)
7
278–281 20 3
2
0.615 (0.500)
0.455 (0.700)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
65 66
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Taihangia rupestris
Taihangia ciliata
TrSSR66
h
F:ATAATAACAGCCAGAACGGAG
R:CGAACGAATGAAAAGGACC
(AAG)
6
268–274 20 3
3
0.636 (0.500)
0.524 (0.450)
Taihangia rupestris
Taihangia ciliata
TrSSR83
h
F:GTCAACCTCAGACCAAAACAA
R:ATACAACGCAACTGCCATACT
(GAA)
6
163–169 20 2
1
0.469 (0.550)
0.000 (0.000)
Taihangia rupestris
Taihangia ciliata
TrSSR85
h
F:ACTCGTCGTCATCCCGTCTAT
R:GTAAGGGTCTCGCCGTATCG
(AAGG)
5
267–275 20 3
3
0.531 (0.600)
0.466 (0.650)
Taihangia rupestris
Taihangia ciliata
TrSSR88
h
F:CGCCAAAAGGAATCTAAGTC
R:AGTAGGGCAGAAGAAAAGTGA
(GT)
7
199–203 20 2
3
0.320 (0.400)
0.515 (0.400)
Taihangia rupestris
Taihangia ciliata
TrSSR90
h
F:TCGTTGAGGGAAAGTGGTTA
R:TATCGCAAATCGGGAAAGA
(TC)
7
259–265 20 3
4
0.445 (0.300)
0.679 (0.500)
Circus cyaneus 00703
i
F:TAGACAGGTAGGTAGGTGGG
R:GGGTCCAGCCTGAAAATTGG
(AGAT)
11
174–207 63 9 0.794 (0.830)
Circus cyaneus 43895
i
F:CCTGTGCAGCTTGTGTATAGC
R:CCGGTCTCAGAGGAACAGATG
(AGAT)
12
148–176 63 6 0.667 (0.680)
Circus cyaneus 04693
i
F:CATTGTGCTATTTCATATGTTTCTGGTG
R:TGTTTCCTTGGTAGGATGGC
(AAAC)
10
241–253 62 4 0.484 (0.610)
Circus cyaneus 22316
i
F:CTGAGGTCAAGAATGGGCTAC
R:TCCATGGGCTGTATGTCAGG
(AAAG)
10
240–291 62 13 0.968 (0.896)
Circus cyaneus 62369
i
F:GATTTAGCGCAGTTTTGTTCCTG
R:GTCTTAAACAAGAGACAATCTAGGGG
(AAAC)
11
112–133 63 6 0.698 (0.672)
Circus cyaneus 55457
i
F:GGGGACCTCTTCAGCTAACC
R:CAAAAAGTGGAAAGTAACACCGAGG
(AAAC)
8
105–113 63 3 0.413 (0.442)
Circus cyaneus HH09-C1
i
F:GGAAGCAGACTAAGTAACCTTG
R:CAGTGTTAGGATTAACCTAAGCTG
(AAAC)
3
GAAC (AAAC)
5
254–266 62 4 0.677 (0.654)
Circus cyaneus HH09-C3
i
F:CTGAACACAGGTTAGAATTTGATTTGC
R:AGATGCTGGTCTGAAGTTTGC
(AAAAG)
5
236–241 21 2 0.143 (0.136)
Circus cyaneus HH11-G7
i
F:TGTATATTCCACTGAGACAGCTG
R:TTGGTGTTTCAAGGATGCATG
(CAGCTTTCTTT)
10
132–199 63 7 0.667 (0.739)
Circus cyaneus HH10-B6
i
F:CTGACCTGCCATTCCAGAAC
R:GCTCATTTCCTCGGAGACAC
(AACAG)
3
AAC (AAACC)
3
145 20 1 0.000 (0.000)
Circus cyaneus HH11-B2
i
F:TACATCTATTCAGGATGGAGGC
R:GGAACATTAGCTTGTGATTGC
(GAAAGGAA)
3
(GGAAGAAA)
5
258 20 1 0.000 (0.000)
Circus cyaneus HH13-E12
i
F:TCAAAAGTTGTGAGTGAGGCTAC
R:GTGATTCAGATGCTCCCTGG
(AGAT)
4
163 22 1 0.000 (0.000)
Circus cyaneus HH13-G11
i
F:CCTCTATCCCAAACTGCA
R:GCCATTACTAACTCTTGGAATTC
(AGAT)
2
AGGTAGGC (AGAT)
5
119 22 1 0.000 (0.000)
Circus cyaneus HHIEAAAG14
i
F:GGGGAGAAATTGTGACAGG
R:TAGTTCAAGGGGAAGGGC
(AAAG)
28
174–244 54 18 0.870 (0.924)
Circus cyaneus HHHf-C1D10
i
FCTCCTGCTAGAGTCAAGAAG
R:GGTAATAATTTCTGTCAGAATGTGC
(AAG)
5
AGG (AAG)
32
179–265 48 12 0.688 (0.773)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
67 68
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Circus cyaneus HHHal 03
i
F:CCTGTGTCAGCTTGGGAG
R:GCATCCCTTCAATCTGTTACACTTC
(AAAC)
5
115 20 1 0.000 (0.000)
Circus cyaneus HHHal 14
i
F:TGCAGCTCTCTTGGACAC
R:TTTCAGCGATGCTCCAGC
(AGG)
10
99–115 63 4 0.667 (0.668)
Circus cyaneus HHHal 16
i
F:CACCTAGAGATAACAAGCATGTAC
R:TCTATACGCAACTTGATGGTTC
(AAAC)
7
(AAAACC)
3
111–115 53 2 0.058 (0.057)
Circus cyaneus HHBswB220w
i
F:GCAAAGCCCACTAGTACAAG
R:CCTTCGTAAACCCCTACTAATTC
(AAT)
15
86–110 63 9 0.730 (0.846)
Circus cyaneus HHBswB221w
i
F:ATTTTCCATATTACGGAGTAACGTC
R:GCAAAAGTAACTCACTTTACTGG
(AAT)
2
(GAT)
2
(AAT)
5
141 21 1 0.000 (0.000)
Circus cyaneus HHBswD220w
i
F:AATAATCATTTACAACACAGAGATGG
R:GACAGATGATTGTAGTAGGTCC
(ATGGA)
15
(GAAGA)
34
252–394 46 24 0.870 (0.923)
Circus cyaneus HHBswD223w
i
F:CCACAGGCAAAAGAAAGAGAG
R:CATTTTAGGTCCCTTCCAACTG
(AAGAG)
9
GAG (AAGAG)
63
382–469 25 11 0.120* (0.844)
Circus cyaneus HHBswD327w
i
F:GAATGGGAATGGAACAGAATATTG
R:GGTTTGCAGATGAATGACAC
(AAGAG)
18
AA (AAGAG)
10
128–258 41 21 0.902 (0.896)
Epinephelus awoara YEGR-8
j
F:#ACTGTCCAAAAGTATGTG
R:GGACAGCCACCATCCTAT
(ATCT)13 116–176 30 13 0.6000 (0.9208)
Epinephelus awoara YEGR-9
j
F:AAGTATGGCAGCGACACC
R:#CCACAACCACAGCAAAGC
(TATC)9(TGTC)7 236–300 30 15 0.7407 (0.9043)
Epinephelus awoara YEGR-11
j
F:GATAGATGGGTTAGGTAGGTTGG
R:#CTGATTCACTGTTTAGACATTCGT
(TAGA)27 187–247 30 15 0.6429 (0.9357)
Epinephelus awoara YEGR-17
j
F:#CGGCGGAAGGAGGTT
R:AGGCAGGAGATACAGGTCA
(TAGA)7 257–305 30 11 0.4815 (0.8700)
Epinephelus awoara YEGR-19
j
F:#TCTTGTGACTTTGGGGACT
R:GGCTTTAGCATTTGGTGTA
(TCTA)11 342–414 30 16 0.8667 (0.9305)
Epinephelus awoara YEGR-20
j
F:GCAGACAAAAAGACAAACACGAGATA
R:#CATACATCCAACCTGACCACC
(TAGA)9 52–136 30 14 0.4828 (0.6776)
Epinephelus awoara YEGR-22
j
F:GTGATGTTGGCTGTTTGTGC
R:#TGCCCTTTATCTTGATTGTGGA
(CTAT)5 115–207 30 14 0.4091 (0.9070)
Epinephelus awoara YEGR-24
j
F:#CAATTTGAGTCTGTGCTGC
R:GTTTGTGTTGTTTTGTAGGCT
(GATA)8 228–292 30 12 0.8276 (0.8966)
Epinephelus awoara YEGR-26
j
F:ACTGCCCAAGTGCCAGAT
R:#AACCTTACAAGGGAGACTCAAA
(TCTA)17 207–267 30 13 0.5833 (0.9202)
Epinephelus awoara YEGR-28
j
F:AGGGTCCAAAGGACAGAGG
R:#TGCTCCAGGTGTTAGAACTCAG
GATT 232–260 30 11 0.7931 (0.8863)
Epinephelus awoara YEGR-30
j
F:#ACATGACAAACCTGGGACT
R:CGATTGGTAGACAGCGACA
(TCTA)22 194–338 30 10 0.3913 (0.9064)
Epinephelus awoara YEGR-31
j
F:ACCCACGCTTACACAATG
R:#TACCTCTCTTTACCCCCTT
(AATC)7 207–244 30 12 0.8000 (0.8345)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
69 70
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Epinephelus awoara YEGR-39
j
F:ACATGGAGCAGTGACGGAG
R:#TGAAGGAGATGCTGATGCC
AACA 143–277 30 9 0.7586 (0.8457)
Epinephelus awoara YEGR-40
j
F:#TGCAGTTGGCATCAGCAT
R:TTAAGCACCAGGAAATACATCAT
ATCT, ATCC 163–247 30 13 0.6923 (0.8989)
Epinephelus awoara YEGR-41
j
F:GTTTCTTTGTAGTGTATGTTAAGTA
R:#GTACCTATAAGCCTATAGTCTACCT
TAGA 221–265 30 15 0.6667 (0.7746)
Epinephelus awoara YEGR-46
j
F:#ACAGGCACAGCGTGTTAT
R:GAATGTTATCTGCTTTATGGGA
(GATA)10 104–180 30 16 0.8929 (0.9214)
Epinephelus awoara YEGR-49
j
F:AGTGGGCTCTTAGCGTGGAT
R:#CCGCTTAGAGTGCCGAAA
(TCTA)12 202–278 30 13 0.5833 (0.9202)
Epinephelus awoara YEGR-56
j
F:#GCTGTTGTAATGCCAGATG
R:GTGTCAAAAGAAGGAGGTCA
AGAC, AGAT 162–218 30 15 0.8214 (0.9214)
Epinephelus awoara YEGR-60
j
F:ACAGATGATGAACCACCCA
R:#ATGCTAATTTCCGCCCTA
TGTC, GATA, TCAT 384–452 30 12 0.7143 (0.7340)
Epinephelus awoara YEGR-65
j
F:#TGGCGTCATCCCTCTGG
R:GCTTGAACTACTCTGCACTGTCA
(TACC)4(TATC)27 168–284 30 12 0.5385 (0.9080)
Epinephelus awoara YEGR-67
j
F:#GTGAAATGTTTGGAGG
R:AAGAAGGAGGTCAGATTGC
AGAC, AGAT 151–248 30 14 0.5909 (0.9323)
Epinephelus awoara YEGR-70
j
F:CCGTAGGAGATGACAAAGAAAT
R:#CCTCTTGCAGCAGAATCCCA
(CTAT)29 228–312 30 15 0.3600 (0.9331)
Epinephelus awoara YEGR-71
j
F:#TGGTGTTTTCAAGTGCTCA
R:GTCCTGCTAGTTGTCCTGTT
(TATC)15 293–365 30 12 0.5667 (0.8463)
Epinephelus awoara YEGR-72
j
F:CCTGTCCATCACCCTTGT
R:#CCTTTGTCAGTGCTTTGC
(TAGA)15 195–243 30 11 0.6154 (0.9163)
Syngnathus schlegeli HL-1
k
F:CGAATGGTTGTATGTCTCTGT
R:AGCATAAGGATGTTTGTCTCA
(ATGG)7 206–264 24 13 0.957 (0.910)
Syngnathus schlegeli HL-2
k
F:CGAATGGTTGTATGTCTCTGT
R:TTGCCCAATAACATATAATGC
(ATGG)4 170–226 24 12 0.522* (0.891)
Syngnathus schlegeli HL-3
k
F:CCCATTGTTTAAATTGACTTG
R:CATATTTCTGACACGTAACTGG
(AC)19 208–278 24 14 0.826 (0.929)
Syngnathus schlegeli HL-4
k
F:GCGAGTTCTAGCAATTACATC
R:CATTCAACAAAGCCTTATGAC
(GATA)21 146–214 24 16 0.917 (0.926)
Syngnathus schlegeli HL-5
k
F:CACCGACAAGATGAATAGAAC
R:ATTGACGGTTGACCAAATAC
(ATCT)38 153–227 24 14 0.995 (0.925)
Syngnathus schlegeli HL-6
k
F:GAATAAGCCACACTAAAGCAG
R:CTAAATTGTGAACCAAATCCA
(TATG)11 206–260 24 10 0.824 (0.868)
Syngnathus schlegeli HL-7
k
F:TGGATGGATGGATGATAGATA
R:TCCCATTTGCTAGTTAGATCA
(ATAG)5 217–261 24 11 0.955* (0.890)
Syngnathus schlegeli HL-8
k
F:GTGACTACTTCCTCTGCTCCT
R:CATTTCAGGTTCACCTATCAG
(CA)15 161–223 24 12 0.810 (0.909)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
71 72
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Syngnathus schlegeli HL-9
k
F:ACTGAGCTGGCCTATCTATCT
R:ACATAGTCTCTCCAGCGTGA
(AG)27 237–289 24 7 0.996* (0.858)
Syngnathus schlegeli HL-10
k
F:ATAAGCGGAAGAAGATGGAT
R:ATCATCTAGTCAGCCATTCTG
(TGAG)20 225–271 24 5 0.375* (0.781)
Syngnathus schlegeli HL-11
k
F:GTAATTGAAGCCCGTCTGT
R:TTGTGCGTGTTTGTCTATGT
(AAAT)15 164–218 24 9 0.913 (0.806)
Syngnathus schlegeli HL-12
k
F:CCGTGAAATAAACAGATGCT
R:GTTTGTGTGTATTGTCCATCC
(TGGA)22 206–250 24 9 0.909 (0.866)
Syngnathus schlegeli HL-13
k
F:TAGATAGACGGACGAACAGAT
R:GGAATATCCACCTTTGGTTAG
(AGAT)32 204–256 24 16 0.998 (0.945)
Syngnathus schlegeli HL-14
k
F:GTCAGAAGCATTAAGACCAGA
R:GGTCATCAATTACAGGCTAAA
(ATA)29 261–285 24 2 0.375 (0.311)
Syngnathus schlegeli HL-15
k
F:CATGGAGAAGAGTCATTGGT
R:GTCATGTATGCACAGATTGG
(CA)25 213–265 24 7 0.792 (0.794)
Syngnathus schlegeli HL-16
k
F:CCCTGCCATTATCTATCCTAT
R:CAGTAAAGGACTGAGGAATCA
(TAA)14 196–239 24 2 0.167* (0.507)
Syngnathus schlegeli HL-17
k
F:TCATCTGCTGATGTTACATTG
R:AAGATAATTGTGCTGTGGATG
(ATAG)23 183–237 24 3 0.292* (0.568)
Syngnathus schlegeli HL-18
k
F:AAAGGCAAGTTAAGACCAAGT
R:GATAATCACTGTGTGCCCTAC
(TCCA)5 251–301 24 2 0.583 (0.507)
Syngnathus schlegeli HL-19
k
F:AATGGTAAATCAAGTTGACGA
R:TATCTGGCCTGTTGATGTAAT
(AGAT)30 226–280 24 4 0.667 (0.752)
Syngnathus schlegeli HL-20
k
F:TCATATATTCGCAGGATTCTT
R:AGATGATGGATGGATGGATA
(ATCC)3 208–266 24 11 0.900 (0.906)
Syngnathus schlegeli HL-21
k
F:GCATGAACGAAATTATTAAGC
R:AAGATGTCAGTGTTTCCTTGA
(AAGA)30 195–251 24 11 0.875 (0.912)
Syngnathus schlegeli HL-22
k
F:ACCGGCACCTAACTAACTAAC
R:TTCATGTGGATATTGAAATCG
(ACTA)19 217–265 24 11 0.875 (0.903)
Syngnathus schlegeli HL-23
k
F:ACCGATCAGTAAGTTCCATTT
R:ATCTACTCCTGGGCTGAAAC
(TAA)3 230–280 24 10 0.792 (0.872)
Syngnathus schlegeli HL-24
k
F:CCTTCAATCAGCAGAGATAGA
R:ACATTACATCCAGCAAACAAT
(AGAT)4 235–257 24 5 0.708 (0.723)
Syngnathus schlegeli HL-25
k
F:TGGATTTAATGATTTGGAGTG
R:CGAATGGTTGTATGTCTCTGT
(ATCC)3 214–254 24 5 0.833 (0.764)
Syngnathus schlegeli HL-26
k
F:GCACCCTCCTAACTGTGA
R:AATTAAAGCAAGAACGGGTTA
(ATCC)4 229–251 24 5 0.286* (0.583)
Syngnathus schlegeli HL-27
k
F:TAGTGACATCAACGCCTCTAT
R:TGATTTGTGTAGCTGATTGAA
(GT)14 209–259 24 8 0.875 (0.876)
Syngnathus schlegeli HL-28
k
F:GCAACTTTAATGAGGATAGCA
R:GAATGAGTCCATGACCAAGTA
(GTTT)13 224–256 24 3 0.333 (0.503)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
73 74
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Galemys pyrenaicus GpyrGS06
l
F:GGGAAAGACCACTATCTGGG
R:TCATCATTCCAGAGCTGCCT
(ATAG)6 191–199 38 3 0.08 (0.08)
Galemys pyrenaicus GpyrGS07
l
F:GAACCACAAACTGAGGGTTCA
R:CCAGCTCATACTGTGTGGC
(CATC)7 194–198 38 2 0.06 (0.06)
Galemys pyrenaicus GpyrGS10
l
F:CACAGCAAGGTCTTAATGAAATAGA
R:GTATCATTTTCTCTGTAAATCCAACTC
(CATC)14 234–246 38 4 0.25 (0.34)
Galemys pyrenaicus GpyrGS11
l
F:CCTGAGGCTCATTCTACTTGC
R:AACCTAAAGTAGAGTAACTG
(CATC)16 198–206 38 3 0.16 (0.20)
Galemys pyrenaicus GpyrGS12
l
F:GTAGCCCACTAGTTGTTGAATG
R:GAAATCCCTGAGGTCAGTAACC
(CATC)7 217–221 38 2 0.05 (0.10)
Galemys pyrenaicus GpyrGS13
l
F:TCTCACACTAACATCCCACTTGA
R:TTCATAAAGTCAATTTAATGC
(CATC)10 174–178 38 2 0.05* (0.19)
Galemys pyrenaicus GpyrGS18
l
F:TTGGGACTGTTATTATTGC
R:TACGGCGGGTGAAATAAGTC
(CATC)15 243–251 38 3 0.13 (0.12)
Galemys pyrenaicus GpyrGS20
l
F:CCTGATGCTGACTTTTGTGC
R:AAGGATCAGACCAAACTCCA
(TCTA)15 219–231 38 4 0.42 (0.54)
Galemys pyrenaicus GpyrGS22
l
F:TGCTTCCTTGTTTTCCCTTC
R:TCAAGGATGGTGCATTTCTGAC
(TCTA)14 208–224 38 5 0.41* (0.56)
Galemys pyrenaicus GpyrGS23
l
F:TTAAGTATATGTACACCCTC
R:TGGCATCTGGTACATAGTAGTTGTT
(TCTA)14 195–211 38 5 0.37 (0.50)
Galemys pyrenaicus GpyrGS30
l
F:GTGTATTAATGTGAATTTGG
R:ACACCCATTCATCTGTTC
(TGGA)16 186–194 38 3 0.09* (0.18)
Galemys pyrenaicus GpyrGS32
l
F:GGTAATAATAGCTCTAAATGCAG
R:TTATTTGCCTCAAGGTCAC
(TGGA)14 189–207 38 5 0.22* (0.60)
Galemys pyrenaicus GpyrGS33
l
F:GGTAGTCAAGGAATTCTCTGCTGT
R:TGCATGTTCCCCCAACTCTA
(GGAT)16 230–238 38 3 0.28* (0.45)
Galemys pyrenaicus GpyrGS34
l
F:CATCATATTTTCTGTGCCTGGA
R:CCTTCTACTCTAGTGATTCAC
(TGGA)16 193–205 38 4 0.33 (0.43)
Galemys pyrenaicus GpyrGS41
l
F:GCAAACCAAGAAGTTATGCCA
R:CAACTTATTTGTTAATGATTTTATCCC
(AG)13 106–112 38 3 0.09 (0.08)
Galemys pyrenaicus GpyrGS46
l
F:GCTCTACACTCTACACGCAG
R:CCTTGACATGAGTATGGGTTCC
(AC)11 186–188 38 2 0.05 (0.05)
Galemys pyrenaicus GpyrGS47
l
F:AGAGTTCTCTAGCATCACCAGG
R:GATCTGGAGGGTTGGGGC
(AC)11 137–143 38 3 0.11 (0.10)
Galemys pyrenaicus GpyrGS53
l
F:TTAGAGAAACTGCATGGGCC
R:TCTTCACATTGTCTGCATCAAC
(CA)10 146–148 38 2 0.09 (0.08)
Galemys pyrenaicus GpyrGS55
l
F:CAAGAACCAAGTGTCTTGCC
R:GTCTGGAGATTCACCCATGC
(AC)10 164–166 38 2 0.03* (0.08)
Galemys pyrenaicus GpyrGS74
l
F:CATAAAGTAGAACTCGTCCT
R:AGGTATGAGTTCTAAGGACC
(CA)15 138–140 38 2 0.05* (0.15)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
75 76
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Galemys pyrenaicus GpyrGS75
l
F:GGTAGAGACTCATCTGAAGGGC
R:TTGTGCCTCCAAATGTTAGC
(CA)10 152–154 38 2 0.08 (0.08)
Galemys pyrenaicus GpyrGS80
l
F:TGATAAGTCAGAATGTGGGG
R:TTTACCTTGACGATCCCTGC
(CA)12 127–129 38 2 0.11 (0.10)
Galemys pyrenaicus GpyrGS82
l
F:CACTATTAGACCAGAAAGCG
R:CAGAGTGTGGCTTACCATTCC
(AC)18 172–174 38 2 0.22* (0.50)
Galemys pyrenaicus GpyrGS94
l
F:AGTTTTGCATAATTAAGCTG
R:GGAGGATGAGCAGTGTATGG
(AC)10 128–132 38 3 0.08 (0.08)
Epinephelus coioides Eco_M2_01
m
F:GTTGGTCATTGGTTTCCCTGAG
R:CCTGCCATCCCCATTGCTT
(AC)
26
305–363 30 15 0.933 (0.919)
Epinephelus coioides Eco_M2_02
m
F:GCATCGGCACCTTCATCTTACC
R:CACAGAAGCAAAACAAGTGGCATTA
(TG)
10
278–316 30 10 0.800 (0.839)
Epinephelus coioides Eco_M2_03
m
F:CAATGTCCAAGTTCTCCATCCAA
R:ACTAATACCTAAACCTAGCCGCTGT
(AC)
15
155–201 30 17 0.900 (0.931)
Epinephelus coioides Eco_M2_04
m
F:GTGCTTTCAGTGGCAACAATCC
R:ACAAATCCCTGTCTTCCTGGTCT
(TA)
11
(TG)
12
150–204 30 12 0.467* (0.823)
Epinephelus coioides Eco_M2_05
m
F:GGATGGGAGGGGCACAGATA
R:GCAGAAAGGGACATAATGAGGAGA
(TA)
10
295–339 30 6 0.567 (0.715)
Epinephelus coioides Eco_M2_06
m
F:TTTTCACCAGGAACTTTATGCTCTAC
R:TCTGAAATTGTATCCGCCCATC
(AT)
11
225–257 30 9 0.433* (0.827)
Epinephelus coioides Eco_M2_07
m
F:GTATTATGTGGGAGGATAGAGTCGG
R:GGACCAGAAGAGGTAATAATGAGGG
(TG)
14
175–231 30 17 0.900 (0.919)
Epinephelus coioides Eco_M2_08
m
F:GAGTGCCAAAGACTGGGTAGAAA
R:CATAGCTGATAGTAGGGTAAGCGAAT
(TA)
15
CAG(TA)
4
193–261 30 8 0.467* (0.716)
Epinephelus coioides Eco_M2_09
m
F:GAACCAGCCAGTTTCTTCCAGC
R:CCACCATCAGCACCATCCAAC
(TG)
7
(AC)
8
G(CA)
13
T(AC)
4
282–384 30 14 0.767* (0.910)
Epinephelus coioides Eco_M2_10
m
F:CAGTGAGGACGAGGTTTGGATG
R:CAGGGGAGTGATCTTAGGCAGT
(AT)
3
AA(AT)
11
375–399 30 7 0.833 (0.818)
Epinephelus coioides Eco_M2_11
m
F:AAAGTGGCTCGCAGAAAAGAAA
R:TGTAACTGTCCGCTGAACTCCC
(TG)
10
189–215 30 6 0.700 (0.764)
Epinephelus coioides Eco_M2_12
m
F:CACCTGTGGCCTGTCTGATTAA
R:TATGTAGGGAGGGACTTTTGTTGA
(GT)
8
GG(GT)
10
(GA)
10
C(AG)
4
200–236 30 9 0.833 (0.892)
Epinephelus coioides Eco_M2_13
m
F:AGTTGTCGTCTTCCGAGGTGG
R:CTTTGCCCCGCAGTGAGTAG
(AC)
4
AT(AC)
25
(TG)
11
434–494 30 11 0.967 (0.893)
Epinephelus coioides Eco_M2_14
m
F:ACTGGCACCTGGATGCTCAC
R:TGCATTATCCTACTTGGCTAAACAC
(AT)
10
245–291 30 14 0.833 (0.920)
Epinephelus coioides Eco_M2_15
m
F:ACACCTTTGGATTGGCTACCG
R:ACCTTTCTGTGCTCCTTTACGC
(AC)
15
GC(AC)
5
375–427 30 14 0.933 (0.931)
Epinephelus coioides Eco_M2_16
m
F:CACATACATCATTTCGTTCACCATC
R:GTCAGTTCAAGTCTGCCCGTTT
(CA)
19
255–297 30 13 0.833 (0.910)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
77 78
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Epinephelus coioides Eco_M2_17
m
F:ACTTCTCAACCTCGACAGTCATCA
R:CGACATCTCCGCCTAAACCA
(TA)
10
375–429 30 14 0.767 (0.858)
Epinephelus coioides Eco_M2_18
m
F:GGTCGGTTGGGGTTGGTTGT
R:GCTTCCTCCTGCTCCTTTTCG
(AC)
12
AT(AC)
5
349–375 30 9 0.833 (0.856)
Epinephelus coioides Eco_M2_19
m
F:TTTCCGTGGAGATTGTTGGTG
R:CAGTGTTGCCGTAAGATGTTGAG
(TG)
14
194–220 30 7 0.767 (0.763)
Epinephelus coioides Eco_M2_20
m
F:AGAAACCAGACCAGAGCCTCTATC
R:AATCAAACTGCTTATGCGTCCC
(CA)
16
(TG)
29
169–197 30 11 0.333* (0.893)
Epinephelus coioides Eco_M2_21
m
F:AAAACCTCCCGCTCTGCTTC
R:ACTGACTCTTCAATATGCCGTCC
(CT)
6
TAA(AC)
19
258–288 30 8 0.933 (0.856)
Epinephelus coioides Eco_M2_22
m
F:CCTCATCACCATCTGGCTCG
R:GTGGGATACAAAACACCCTTCAA
(TG)
19
308–356 30 11 0.667 (0.732)
Epinephelus coioides Eco_M2_23
m
F:CTCTGATTGGCTTTTCTGAGTGATG
R:TGGGTTGGCTGCCTTTGCT
(AT)
10
266–290 30 6 0.567 (0.719)
Epinephelus coioides Eco_M2_24
m
F:CCTCCATCAGTCAGAGTTGGTTAGT
R:TGTTGTCATCAAAAGCACCTTCC
(AT)
11
247–277 30 10 0.767* (0.848)
Epinephelus coioides Eco_M2_25
m
F:GTTTCTGCTCCGTCGTCGTG
R:GTTTCATGCTGTCCAGGTACTCC
(TG)
27
270–306 30 12 0.900 (0.886)
Epinephelus coioides Eco_M2_26
m
F:CTGAGAAGAAGGAAAGGGGACC
R:CGTACAAGCATGTTGGGGAGA
(TA)
19
152–176 30 11 0.833 (0.860)
Epinephelus coioides Eco_M2_27
m
F:TTATCAAGGTTTCCTATTCCCAGTG
R:TGGTTTGCCTTCAGCATTTAGAC
(GT)
15
(GT)
11
367–417 30 10 0.800 (0.894)
Plectropomus leopardus Ple_Trs_01
n
F:CAGCTGGATCATTATCTGATCTCC
R:TTCAGGTGCAGGAGAAAGGG
(TG)
13
151–181 30 11 0.933 (0.899)
Plectropomus leopardus Ple_Trs_02
n
F:TGGTTTATATCCACAGCCCG
R:TGCCTCCTGAACACACACG
(GT)
18
172–204 30 8 0.931 (0.842)
Plectropomus leopardus Ple_Trs_03
n
F:GGGCGAACGAGTGTTTATCC
R:TTTGGGGTCTGTGATTCGC
(GT)
14
155–205 30 8 0.700* (0.843)
Plectropomus leopardus Ple_Trs_04
n
F:ATTACTGAGCTGGCATGGGG
R:CCAGCACTAATAGCATAAGTGACCC
(AC)
2
AA(AC)
6
(CG)
2
(AC)
15
154–196 30 7 0.800 (0.748)
Plectropomus leopardus Ple_Trs_05
n
F:GGTTTCCCAGCAGACATGC
R:TGTCTAGCTACAAACTGTACTCGTCG
(GT)
19
120–148 30 8 0.483 (0.485)
Plectropomus leopardus Ple_Trs_06
n
F:TGAGGAAAGCATTTCACAGCC
R:AAAAGACCACACGTAAACAGCG
(CA)
9
164–190 30 10 0.800 (0.750)
Plectropomus leopardus Ple_Trs_07
n
F:TGAGAGTCTGGAACCAGGGC
R:TTCATCAGGCTGCTCACAGG
(AC)
2
(TG)
5
(TA)
4
(TG)
3
G(TG)
6
155–217 30 10 0.367* (0.692)
Plectropomus leopardus Ple_Trs_08
n
F:GGAGTAAACCGTTGTGTGCG
R:TTGCTAATGGCCCTGTTTGG
(GT)
2
C(GT)
10
172–178 30 3 0.767 (0.593)
Plectropomus leopardus Ple_Trs_09
n
F:CGCTTAAAACCCGCTTTCC
R:AGCTGTTCTCTGGCCTGTCC
(GT)
15
138–158 30 9 0.926 (0.818)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
79 80
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Plectropomus leopardus Ple_Trs_10
n
F:GGGATATGTTCAAGGCAACG
R:GCTGACCCAGTTCAATAATCCC
(CA)
3
CG(CA)
6
CT(CA)
11
126–188 30 11 0.862 (0.820)
Plectropomus leopardus Ple_Trs_11
n
F:GAGGGGTTCAGTTCAGTCGG
R:ACGCTTGCACTCTGGTTAGC
(GT)
18
164–186 30 8 0.933 (0.853)
Plectropomus leopardus Ple_Trs_12
n
F:AGACAGGCACACACAGAGGC
R:CATTCATGGCTAATCAGTCGC
(TG)
10
126–152 30 9 1.000 (0.815)
Plectropomus leopardus Ple_Trs_13
n
F:GCTCAGGAGTGTGAAAGATGC
R:CTGCGTGTGGAGGTCAGC
(GT)
11
113–177 30 10 0.533 (0.819)
Plectropomus leopardus Ple_Trs_14
n
F:AGCTGGAAAAGGAGCAGAGG
R:CTGTGGTGAATGAACACATGC
(CA)
17
168–206 30 9 0.500* (0.757)
Plectropomus leopardus Ple_Trs_15
n
F:GCTGGCAACAGCAAACTGC
R:CTCCAGCATGCACATGATCC
(TG)
24
165–189 30 9 0.833 (0.753)
Plectropomus leopardus Ple_Trs_16
n
F:CCTACGACTGCTACGAGAGATACC
R:TCAACAGACAACAAGCTGCC
(CA)
18
127–147 30 7 0.800 (0.848)
Plectropomus leopardus Ple_Trs_17
n
F:GGGACGAGAGAAACACAGCG
R:CACAGCAGAGGTAGTTAGAGGGG
(GA)
15
(AGAC)
3
(TG)
7
136–190 30 11 0.867 (0.861)
Plectropomus leopardus Ple_Trs_18
n
F:GAGATGCTTTGGCAAGAACTGT
R:ATAGTCCAATGAGGCCAGAAAC
(GA)
39
93–126 30 12 0.165 (0.835)
Plectropomus leopardus Ple_Trs_19
n
F:AAACCCAACAGTTTGTACTGCAT
R:CTCTCCGTGTGCGTAAAAATAAT
(CTAT)
15
116–147 30 10 0.126 (0.874)
Plectropomus leopardus Ple_Trs_20
n
F:AGAGGTTGTCAGTGGGTCTCAG
R:GCTGCCTCTTTAGCAAGATTTTT
(AGA)
13
100–139 30 9 0.118 (0.882)
Plectropomus leopardus Ple_Trs_21
n
F:GACTTGTTCCTCCCGTCCTAC
R:AGGGCTTTAGCCAGGGAGAG
(GGA)
13
110–126 30 4 0.243 (0.757)
Plectropomus leopardus Ple_Trs_22
n
F:TTTTCTCTTGACCTTTACCCCTC
R:AATAAAAACAGGCATGAAGTGGA
(CTCTC)
8
110–135 30 8 0.141 (0.859)
Plectropomus leopardus Ple_Trs_23
n
F:TTCTCTTGACCTTTACCCCTCTC
R:GAGGGAGGAGGAGAATAAAAACA
(CTCTC)
8
115–143 30 6 0.172 (0.828)
Plectropomus leopardus Ple_Trs_24
n
F:ACGAGGAAGGATCTAATCCAGAC
R:CTGCAGGTGTCTCTCATATGTCTT
(TCTGG)
5
144–160 30 6 0.198 (0.802)
Passerina ciris Pb16
o
F:TCACAATGGCACACGAAGC
R:TGCAGACTTCAGGGACGTG
(GTTT)6 275 63 13 0.875 (0.975)
Passerina ciris Pb14
o
F:ATATTCCATGTTCTGATTAGTACCC
R:ACTGAGAAACCTGGTCTAGTGG
(ACAT)4 165 63 3 0.666 (0.485)
Passerina ciris Pb2
o
F:ACCAGGTGATTCTGGTCTTC
R:TGTTGCTTCAGCCCTTTGG
(GGTT)5 235 63 11 0.794 (0.767)
Passerina ciris Pb11
o
F:GGGCTGAGACCTGCTTGG
R:CCCAAACCATTGCCTGTGC
(GGAT)6 193 63 32 0.953 (0.955)
Passerina ciris Pb8
o
F:CAGCCCAAATGTTGGTTTGAC
R:GCACACCAATGGGAGCATAC
(AAAT)6 219 63 9 0.418 (0.508)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
81 82
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Passerina ciris Pb4
o
F:GGATAGGGTCTTCAAGCACAG
R:TGCCTGCTTATGTTATGGGC
(AGAT)5 151 63 14 0.813 (0.809)
Passerina ciris Pb7
o
F:GGCTGAGTTTGGGCTATGG
R:ACATGTGAGACATCACTAAAGGAG
(CTTT)5 256 63 19 0.697 (0.816)
Passerina ciris Pb15
o
F:AGGATTGATGCGTTAGGCTTG
R:GGTCCTGGGCTCACCTTC
(AAAT)5 207 63 4 1.00 (0.716)
Passerina ciris Pb9
o
F:GTGTTGAGAGCCTGATGCC
R:TCCAGGAGTGTGCCTAACATC
(AATG)14 161 63 21 0.767 (0.918)
Passerina ciris Pb12
o
F:CACAGCTCGGTGTCATTGG
R:ACAAACTCCTTAATAGCTGAGGC
(AATC)7 214 63 7 0.534 (0.707)
Passerina ciris Pb5
o
F:TGAGCATGGATCTGCCTAC
R:GGAGAGGAGGGACTTGTGG
(GGAT)5 252 63 20 0.883 (0.849)
Passerina ciris Pb3
o
F:TGAAGTACACATCTGACTCATGG
R:GAGCTCTAAAGTCATGATTAATGCAAC
(AAAC)5 325 63 23 0.953 (0.949)
Passerina ciris Pb17
o
F:GCACAGGTCCATACTGAGAAC
R:TTTCTTTGCAGATGTGAAGGG
(CCTT)4 177 63 2 1.000 (0.600)
Passerina ciris Pb13
o
F:CCGCAATAAGGAGGTGGAAAC
R:ACCTCTGAGCAATTTCTGTAGC
(CCTT)4 190 63 15 0.744 (0.817)
Passerina ciris Pb10
o
F:GCAGTTCAGTTCCATCTGGC
R:TGCAACGTGGCTTTATTTGC
(AAAT)6 238 63 9 0.395 (0.566)
Passerina ciris Pb6
o
F:TCAGAATTACCTGGTGCTCCC
R:ACAGAGCCATTGGTCCAGC
(GAGT)6 207 63 14 0.627 (0.825)
Passerina ciris Pb1
o
F:ATCGATGCAATGGCTGCAC
R:CGTGCCTCAGTTTCCCATC
(CCCT)5 242 63 9 0.674 (0.665)
Passerina ciris Pb19
o
F:CAGCGTTATTGGGTCCATTTAT
R:GTCAACAGAGGATGGGTGATCT
(AC)12 186 63 17 1.000 (0.911)
Passerina ciris Pb18
o
F:CTTCCTCCCACAAAATCAATTC
R:TCCTATGGCTTCTTCTTGTTGA
(AC)15 150 63 11 0.900 (0.830)
Passerina ciris Pb20
o
F:AGAAACATCAGCAGACAGGAGA
R:GGAGCACACAAGATTACATCCA
(AT)10 349 63 18 0.850 (0.896)
Brycon orbignyanus Borb01
p
F:TCTGTCTATCTATCTGCCTATCTGTTGA
R:AGGAGGCATAGTGGTAGCATGTAG
ATCT 100–135 46 8 0.435* (0.826)
Brycon orbignyanus Borb04
p
F:TTGTGAACTATGATTCCAACTGTATGTC
R:GGCCTCATAACCCTTTCCTAACTT
ATAG 118–123 48 2 0.208 (0.219)
Brycon orbignyanus Borb06
p
F:AGGTGGGTCTTCAATTATTAGCAA
R:GCAACAATAAACACAACAATAATGCTAC
ATT 110–137 49 6 0.612* (0.812)
Brycon orbignyanus Borb07
p
F:TCAGTGCTCTGTGTGCATTTTTATT
R:AAGGAAGTTTAATGTTGCCAAACG
ATA 142–152 49 5 0.163* (0.716)
Brycon orbignyanus Borb08
p
F:AGGAAGCGGAGAAATGTGTATTGA
R:GGGAACATGATAAAAGTGCTGCAT
ATT 114–140 49 8 0.633* (0.792)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
83 84
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Brycon orbignyanus Borb09
p
F:TTTAACTCCTTCAGTCTGCACAGC
R:GTGCGCCTACAAATAAACAGAACA
AAT 126–144 49 5 0.694 (0.717)
Brycon orbignyanus Borb11
p
F:TCACCAACCAACAGAAAACACAAT
R:CATCTGAGGCCTGTTTGGTCTTAT
ATT 147–178 48 8 0.688* (0.806)
Brycon orbignyanus Borb12
p
F:CAGATGTTATTACACGTTTTGTTGCAC
R:TGTGAGTGTTTGTGAGTGTTTGTGA
ATT 118–161 48 8 0.625 (0.836)
Brycon orbignyanus Borb13
p
F:ACATATGGTCATATTGCCCACACC
R:TGCTCCAACATAATACACAAAATGATG
ATT 103–137 49 7 0.551* (0.720)
Brycon orbignyanus Borb14
p
F:TCCCAAAATAATGACTCAGCAGAA
R:TAGCCTCTCTCACAGAACAATGGA
ATC 116–154 49 7 0.694 (0.766)
Brycon orbignyanus Borb15
p
F:CGTAGCCTCATTGTGTAGCATAGC
R:GCCATCACTACAGTCTTTTAACCCA
ATT 147–179 41 9 0.390* (0.865)
Brycon orbignyanus Borb16
p
F:GCACGAACCAATACTAGGAAATGC
R:TGTAACAAAGAAACACACTGTCAGCTC
AAT 124–172 48 8 0.521 (0.681)
Brycon orbignyanus Borb17
p
F:TCAAAAAGCAAAAAGTTTAAAACATGC
R:ATGTGGAATAAAGGGCAAGACAAA
ATA 131–151 49 6 0.612 (0.788)
Brycon orbignyanus Borb18
p
F:AAAGGCACAGTAGCAAAAGTCTGTTTA
R:AACGTCTCGTGAGTGTCATCTGAG
ATT 142–175 48 7 0.500* (0.786)
Brycon orbignyanus Borb21
p
F:AGAGGCGGAGAGGAGATCTAAGAA
R:GTTGCCAAGGAGACAGCAGAGT
CA 101–127 49 7 0.673* (0.761)
Brycon orbignyanus Borb24
p
F:GAAAGCTTACTGTTGGTCAGAGGG
R:GCTCCAGTAACTTGTACATTTGCG
AC 96–104 49 4 0.469 (0.542)
Brycon orbignyanus Borb25
p
F:CAGCAGTGATTGAGTAGTCATGGG
R:CCTGCTCTCCACAGAGGGTTATTA
AC 262–318 48 9 0.979 (0.817)
Brycon orbignyanus Borb28
p
F:CCCTCCTTTTCTTCCTTGCTTTAT
R:GCTTTTGATTACAGGAGCATGGAT
CA 123–141 49 4 0.306* (0.611)
Brycon orbignyanus Borb29
p
F:CACTGTATGCGGAGCAATTTACTG
R:TGTACACCTACAGTTGTGTTTGTGTTG
AC 112–128 49 6 0.551* (0.744)
Brycon orbignyanus Borb30
p
F:CACTGCATATGTCCAAACTGAACTT
R:GCTCTGTGTTCCAGACAGGATCT
CA 79–89 49 4 0.245* (0.737)
Brycon orbignyanus Borb33
p
F:CCAACCAGCTGAGTCAGTCAGTA
R:GCTCTATGGCTCTATGTTCTAACGC
AC 68–83 49 3 0.510 (0.532)
Brycon orbignyanus Borb34
p
F:AGCCCAACACAGCAACAGAAA
R:CTTTCAGCCCTACTCCCACG
AC 89–120 49 8 0.898 (0.830)
Brycon orbignyanus Borb35
p
R:GGGCGTTAAAACGTAAAACTCAGTC
F:CTTTACGTCTCTCACTCACTTGCG
AG 114–138 49 7 0.755 (0.820)
Brycon orbignyanus Borb36
p
F:GGAAAGAGAAAAGGGGAGAAGACA
R:CATGCCCTCTTTCTAGGTGAGTTC
AG 89–109 49 6 0.592* (0.785)
Brycon orbignyanus Borb38
p
F:ACTGACAGACAGAGCACTCACACTC
R:AAGTAGCTTGTCTGGTTGCAAAGG
CA 80–112 49 8 0.694 (0.810)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
85 86
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Brycon orbignyanus Borb39
p
F:CATAATCGCTCCAGTGCTTACTCA
R:TTAGAACAAATACGCTTGGCTCAAC
AC 94–101 48 2 0.292 (0.278)
Brycon orbignyanus Borb43
p
F:AGAGAAACGGCCCTTCAGGAT
R:AAGCTTTTCTCCAAACGTCCCTAT
AC 113–148 49 7 0.653 (0.778)
Brycon orbignyanus Borb44
p
F:CCAACAACGACTCCATTTACACAC
R:ATTCATGGATTCTCTTCTCCTCCC
AG 88–110 49 5 0.633 (0.687)
Brycon orbignyanus Borb46
p
F:ATGCGTAACCCTGATCTAGCAATG
R:TGTCAAAATGTGTGCCTTTCTATGTT
CA 121–131 49 3 0.224* (0.653)
Megalobrama amblycephala Me.AM_1
q
F:AGCGTGAGGATTCCAATT
R:AGAGGCAGAAACAACAGA
(AGAAG)5 210–275 90 7 0.8889 (0.8371)
Megalobrama amblycephala Me.AM_2
q
F:ACCTCGTTGGCGTAGC
R:CTCAAGCGCAGTATGG
(GATCA)5 200–222 90 5 0.3333 (0.7789)
Megalobrama amblycephala Me.AM_3
q
F:AGAGGCGGCCAAAATCAGAA
R:CCAACCAACCAACCAACCAA
(CTGA)5 200–225 90 3 0.9444 (0.6320)
Megalobrama amblycephala Me.AM_4
q
F:ACTAAGAGTGAACTACGCTA
R:TGAAAAATCAAAGAAAAAAT
(TCAT)5 187–213 90 4 0.9778 (0.6918)
Megalobrama amblycephala Me.AM_5
q
F:CCACTTTTTTTTATTTGA
R:CTTGTGCTGCTCTCTCTC
(TTCA)5 155–172 90 4 0.9333 (0.6995)
Megalobrama amblycephala Me.AM_6
q
F:TAAAATCAAAACAGACAA
R:ATAAAAACAAAACACACA
(TAAA)5 105–153 90 3 0.8764 (0.6647)
Megalobrama amblycephala Me.AM_7
q
F:CCAAAATGATTCAACAGCCT
R:TGTAACAGCACTTTCCCCTA
(TTTC)5 296–312 90 4 1.0000 (0.7444)
Megalobrama amblycephala Me.AM_8
q
F:TTAGCACAGAAGGGGAAG
R:TTGAAAGCATTTGAACAG
(ACAT)6 223–264 90 5 0.9874 (0.7675)
Megalobrama amblycephala Me.AM_9
q
F:ACAAGCCAAGTGTCCTCC
R:TTTCTTTCCAATGCAGCA
(GAAA)5 275–326 90 4 1.0000 (0.7515)
Megalobrama amblycephala Me.AM_10
q
F:TCTTGCTTTATTGGAACC
R:AACAACACACACATCTGC
(GGTT)5 267–289 90 4 0.7333 (0.7504)
Megalobrama amblycephala Me.AM_11
q
F:GGAGATGCTGGTTTGACTGG
R:AGATGGAGATGGACAAGGGG
(CCTT)5 212–275 90 5 0.9667 (0.7952)
Megalobrama amblycephala Me.AM_12
q
F:ACAGCAGCATAAAATCCAGGA
R:AGTCTTTGTTTGAGGCAGCA
(ATAC)5 194–267 90 7 1.0000 (0.8158)
Megalobrama amblycephala Me.AM_13
q
F:TCCCAATGATGTAGTGCAAGT
R:CCTGATGTCATCTAGCCAAAGG
(TAAA)5 185–234 90 4 0.3333 (0.5226)
Megalobrama amblycephala Me.AM_14
q
F:AGCATGTTTTCGGGAAGCTG
R:CATGGAAACGAGGCAGCTTT
(TGTT)5 157–184 90 4 0.2667 (0.5476)
Megalobrama amblycephala Me.AM_15
q
F:TGTGGATGCCCTGAGTGAA
R:AATGTGTTCGTGTGGAGAG
(CATT)5 175–200 90 3 0.7000 (0.5327)
Megalobrama amblycephala Me.AM_16
q
F:CAAAAGTGAAAGGGGCGGAT
R:AATCACAGTTGCCAGTCACA
(AATA)5 147–158 90 3 0.8000 (0.5492)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
87 88
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Megalobrama amblycephala Me.AM_17
q
F:AGGCGAAAGAAACACTGTGT
R:GGTGTTCGTGCGATGTTGTA
(TGGA)5 180–320 90 3 0.3000 (0.5949)
Megalobrama amblycephala Me.AM_18
q
F:CAGCATATCCCGATCCCAGT
R:GAGGCAGCGACGTTAGTTTC
(TC)6…(CT)6 195–266 90 7 1.0000 (0.8158)
Megalobrama amblycephala Me.AM_19
q
F:AACTGCAGCCCACTCTATGT
R:GCTGTTCTTGTTGTGGCTGT
(ACC)5(AAC)5 185–225 90 3 1.0000 (0.5542)
Megalobrama amblycephala Me.AM_20
q
F:AACACAACAGAGCTGCCAAG
R:TCTCTTCAAGAACATGCGCC
(GT)7…(GT)7 151–164 90 4 0.2667 (0.5746)
Megalobrama amblycephala Me.AM_21
q
F:ACCAACCACTTGTGAGGAGA
R:GGTTTGTCTGCTGGTCTTCC
(CT)8…(CT)6 18–258 90 4 0.3333 (0.5226)
Megalobrama amblycephala Me.AM_22
q
F:TTCTGTGTGTGTTCGCGAG
R:AGGCACCAGACAAGGAGTT
(TG)6…(GC)7 191–206 90 3 0.2667 (0.6119)
Megalobrama amblycephala Me.AM_23
q
F:TCGGATCTCTGTGCTGTGTT
R:GCCACTGGAACTGAGAGACT
(TC)8…(CT)9 191–212 90 5 0.7000 (0.7079)
Megalobrama amblycephala Me.AM_24
q
F:CTGGTCGACGTTTAAGGTTCC
R:CTGATTGGCTGAAATGTCCGT
(AAC)5…(AT)7 160–362 90 3 1.0000 (0.6215)
Megalobrama amblycephala Me.AM_25
q
F:ACGAGTCCAGACACACGAAT
R:ATTCCGAACCGAAGCAACAC
(ACA)5…(GT)8 200–250 90 5 0.9667 (0.7079)
Megalobrama amblycephala Me.AM_26
q
F:CAGAAGGAACGAGGGGTCTG
R:CAGCAAGAAGAAGAGGCGAC
(GT)7…(GT)6 214–224 90 3 0.1023 (0.5605)
Megalobrama amblycephala Me.AM_27
q
F:ACATTTGCCGTCTCGAAAGG
R:TCCTGGAAACTTGAGGGACG
(TC)6…(CT)7 173–187 90 3 0.6000 (0.5712)
Megalobrama amblycephala Me.AM_28
q
F:AAAGAGGGAGAAACGAGCCA
R:TACGGCAGCGGAAATAAACG
(GT)6…(GT)9 151–159 90 4 0.5000 (0.6537)
Megalobrama amblycephala Me.AM_29
q
F:GAAGCCACTTAACCCATGGC
*R:TGCCTCTGTAGTGTAGTTGCA
(CA)7…(CA)10 183–217 90 6 0.3000 (0.6763)
Megalobrama amblycephala Me.AM_30
q
F:TAACTTGCTGTCACCGCTTG
R:TCTCCTAAGCCTTGCACACA
(TG)6(TGT)6(T)12 192–212 90 3 1.0000 (0.4249)
Megalobrama amblycephala Me.AM_31
q
F:ATGACAGCACAACACAGCAG
R:CACACACACACTCACTCGC
(AC)6…(TCA)5 235–370 90 4 0.5567 (0.4209)
Megalobrama amblycephala Me.AM_32
q
F:TGTGTGTGTCTGTATGTGTCCT
R:TGGCCTTCTTCCTGTCTGAG
(CA)8…(AT)6 220–245 90 5 0.1333 (0.6237)
Megalobrama amblycephala Me.AM_33
q
F:CTGCCTTCAAACACCCAACA
R:TGTTTGTCTGGTCATGCAGC
(AG)10…(TG)6 200–247 90 5 0.1333 (0.6237)
Megalobrama amblycephala Me.AM_34
q
F:TCTGCGGTGCTGTTTGAA
R:GGCCCGAGACGATTATAA
(CT)8…(AT)6 200–287 90 4 1.0000 (0.6797)
Megalobrama amblycephala Me.AM_35
q
F:GACGACTGAGGGGTTAGAGG
R:AGCGAGTGATGCCAGAAAAC
(AC)9…(GT)7 123–212 90 6 0.7333 (0.7751)
Megalobrama amblycephala Me.AM_36
q
F:TCTCGATGGACACGTATGG
R:AGCATCTCTGGTTGTGGACA
(GT)9…(GT)7 124–247 90 8 0.9667 (0.8271)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
89 90
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Enhydra lutris nereis EluA011w
r
F:TGCCCACTCTGTGTTTCTCTTA
R:TGTGTGTGGCATATGTGAGTGT
(CA)19 205–221 30 4 0.50 (0.64)
Enhydra lutris nereis EluA109w
r
F:GCGTCCCGACTCTGGTATTT
R:AGACAGAGAAGGGAAAGGAGGA
(CA)8.5G(CA)13.5 235–249 30 3 0.53 (0.48)
Enhydra lutris nereis EluA202w
r
F:GAGAGACAGGCAGAGCAAGTTT
R:GAAGTGTCAAGAAGGAGCTGGT
(AC)26.5 252–280 30 5 0.50 (0.57)
Enhydra lutris nereis EluA309w
r
F:GGCCTCCTCTCCAGGTTATATT
R:ACAAGCAGTTCTCCCTCTCATC
(AC)18.5 206–214 30 4 0.43 (0.37)
Enhydra lutris nereis EluA312w
r
F:GCAGCGACACAGACACAATTA
R:AATGGAACGGGAAAGTTCTG
(TG)20.5 237–241 30 3 0.57 (0.58)
Enhydra lutris nereis EluA314w
r
F:AGCCCAAGTGTCTATCCCAGAT
R:CCCTAGATCCATCTATGTTGTTGC
(CA)21 149–163 30 3 0.57 (0.50)
Enhydra lutris nereis EluA315w
r
F:AAGAAGGCAGCAAATACCTCTG
R:CCTGAACTCAGCCCTATGTTTC
(GT)25A(TG)5.5 195–215 30 4 0.63 (0.69)
Enhydra lutris nereis EluA316w
r
F:AAATCCGTAAGGGTGAAGCA
R:ATTAACCACGTCGCCTCTGTAT
(GA)16(GT)12 204–210 30 3 0.57 (0.51)
Enhydra lutris nereis EluA318w
r
F:CGTCTGGGCTAGTCACCTCTAA
R:AGGCTAAGCTGCTGTGTGAAAG
Enhydra lutris nereis EluA324w
r
F:GAGACCTCTCTCTGAGCCATCA
R:CTCACCTCATGCCATCTCCTAT
(AC)16.5 242–246 30 3 0.53 (0.50)
Enhydra lutris nereis EluA325w
r
F:CACGAACGAAGACGTGACTAAG (AC)22 156–172 30 4 0.63 (0.65)
Enhydra lutris nereis EluB110w
r
F:TCAGGTGTGTATGGAGATGAGTG
R:GAGCCTTCCAGAGAGAATGAGA
(TC)20 160–170 30 4 0.80 (0.72)
Enhydra lutris nereis EluB118w
r
F:GTCCAAACCAGAACCAGAGC
R:ATTATAGGCTGCCCAGTTACCA
(TC)13.5 231–233 30 2 0.13 (0.12)
Enhydra lutris nereis EluB122w
r
F:ACCTGATGTAAAGGGTGAGTGG
R:GCTCTGAATTAGCTCGGTAGGA
(TG)15(AG)18 206–210 30 3 0.63 (0.62)
Enhydra lutris nereis EluB302w
r
F:CTCAGACTCACCTGTTGCTCAC
R:AGGAGGCTGGAGTGTTTGTTTA
(AG)17(TG)16.5 219–227 30 3 0.37 (0.31)
Enhydra lutris nereis EluB312w
r
F:CGCAGCTTACCATATAACCAAG
R:CCTTGAATGTGACTGCTCAAGT
(AG)16.5 160–168 30 5 0.53 (0.55)
Enhydra lutris nereis EluB313w
r
F:CAACAACTGATTCTGGCTGAGA
R:CTCAGGTCATGATCCCAGAGTT
(GA)16 278–282 30 2 0.23 (0.30)
Enhydra lutris nereis EluB317w
r
F:GCTGCCTCACCCAAATATAGAC
R:GGTGTCGATGCTGAAAGAGTAA
(TC)17 243–251 30 3 0.47 (0.45)
Enhydra lutris nereis EluB326w
r
F:TTCTCTTCCTGTGACCCTCATT
R:GCAAGAGTATGCAGATGTGAGG
(TC)13.5 235–245 30 3 0.57 (0.52)
Enhydra lutris nereis EluD011w
r
F:TACATAGGCGGTCTCCTTCTTC
R:CCTGCTTGTGATCTCTGTCTGT
(TTTC)5.5(TCTA)7.25(TTAT)2.5 236–264 30 7 0.70 (0.78)
Enhydra lutris nereis EluD119w
r
F:AGCCAGTAGGAGATGAAAGCAG
R:GAGTAACACCTGGGCCTCTCTA
(GATA)10.5 232–240 30 3 0.47 (0.59)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
91 92
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Enhydra lutris nereis EluD219w
r
F:TACTTGGATTTCTCGCTCTCCT
R:ACGTTTCATCCACCTGTCTCTT
(ATCT)11 178–186 30 3 0.47 (0.59)
Enhydra lutris nereis EluD311w
r
F:GTCACATGCATTCTTCCCAGT
R:CTCAGGGTCATGAGATCTAGCC
(TCTA)9.5 171–179 30 2 0.40 (0.49)
Enhydra lutris nereis EluD320w
r
F:TCCCACTCAGGCTTATAAATGG
R:TCTGCTTGGAACATGTTTGG
(ATAG)13.75 200–212 30 4 0.70 (0.66)
Takifugu fasciatus TF9
s
KT768017
F:AGGACAGAGGCAACCCTTTT
R:AGGCTCAGATGCGTTTAGGA
(CT)9 284–294 32 5 0.750 (0.698)
Takifugu fasciatus TF 10
s
KT768018
F:TCTCCGGGTGTGATTTAAGG
R:CTCGGCCAATGGAAAATAAA
(AC)8 262–276 32 6 0.469 (0.800)
Takifugu fasciatus TF 11
s
KT768019
F:GAAGCAGCTCTTATCTCCGTG
R:CATGCCAGATCAACCATCAG
(AT)8 214–226 32 4 0.563 (0.586)
Takifugu fasciatus TF 21
s
KT768020
F:AGCCAACCTTGGAGGAAAAT
R:TAAATGTGCGACACGAGAGC
(AG)8 270–274 32 3 0.406 (0.431)
Takifugu fasciatus TF 31
s
KT768021
F:AGGTGGACAGTGTGCATTGA
R:CGAGGAATCTGAAAAGCTCG
(TG)8 282–284 32 2 0.219 (0.329)
Takifugu fasciatus TF 34
s
KT768022
F:AGCTCGGAAAATACAGCTCG
R:CCTTCTGAAACAGGAGGACG
(AC)9 258–264 32 4 0.594 (0.695)
Takifugu fasciatus TF 35
s
KT768023
F:AGGTTTTTGGAGGACCAAGC
R:GCGGCTAGTCTCAGGTTTGA
(GT)9 284–290 32 4 0.313 (0.305)
Takifugu fasciatus TF 41
s
KT768024
F:CACACGTCTGTGGAGGATTG
R:CCATCCCACAGGAAGAAAAA
(CTT)5 270–275 32 4 0.531 (0.617)
Takifugu fasciatus TF 57
s
KT768025
F:AGGCAGAGTGCACGGATCTA
R:ACTAGAGGGCAGTGTTGCGT
(AAT)5 255–267 32 4 0.625 (0.607)
Takifugu fasciatus TF 58
s
KT768026
F:GATGAGACTCCTGCGAGACC
R:ACAGTCGAGGCATCTGACAA
(ATT)5 261–273 32 4 0.219 (0.282)
Takifugu fasciatus TF 63
s
KT768027
F:CATCAACGCCTTCCAATAGC
R:CTGACCCATATCGGTTGCTT
(TCA)6 211–217 32 2 0.250 (0.268)
Takifugu fasciatus TF 78
s
KT768028
F:AGACTAACCCTGCTGCTGGA
R:CAAGCTAAAGGAGAGCACGG
(TCT)5 240–243 32 2 0.406 (0.424)
Takifugu fasciatus TF 87
s
KT768029
F:CGCAGGAAGACACCTTTCTC
R:TGAAGACGTTAACCTTCGGG
(AGG)5 286–289 32 2 0.156 (0.289)
Takifugu fasciatus TF 91
s
KT768030
F:AACAGCATCCTCACGAAACC
R:CACATTTATCACGCCAGGTG
(CCA)5 287–293 32 2 0.313 (0.268)
Takifugu fasciatus TF 92
s
KT768031
F:AGACTCAAACAAGGAGCCCA
R:ACATTGCGCTGTAGGAAACC
(TAT)5 292–298 32 3 0.500 (0.520)
Takifugu fasciatus TF 96
s
KT768032
F:TTCCCATCCATCAGTTTTCC
R:TGGGGTCATTAAAAACCTGG
(TAA)5 251–257 32 3 0.281 (0.294)
Takifugu fasciatus TF 98
s
KT768033
F:GGAGGCTCTGATGACTGAGG
R:CATCAACCACAACAATCCCA
(TGT)5 243–246 32 2 0.531 (0.396)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
93 94
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Takifugu fasciatus TF 99
s
KT768034
F:CGGCTGAAATGTTTAACGGT
R:TGACCTGGCAGTTTCTGGAT
(TTC)7 259–262 32 2 0.313 (0.381)
Takifugu fasciatus TF 101
s
KT768035
F:CACACAACGCTCAGTCCCTA
R:CCGGCTTCAGAGAAACTGTC
(TAA)6 247–262 32 5 0.688 (0.612)
Takifugu fasciatus TF 110
s
KT768036
F:TGGCATTCAACTGGTGTCTC
R:TACTTGGCGGAATTTTGGAC
(GAAA)6 289–297 32 3 0.625 (0.598)
Takifugu fasciatus TF 113
s
KT768037
F:ACGTACATGGTCGAGGAAGC
R:GGAAGATCAAACACCTCCCA
(GGAC)5 237–250 32 5 0.750 (0.691)
Takifugu fasciatus TF 115
s
KT768038
F:TGTCAGGGAGACAAAGGACC
R:AAACAAACAAATTCGGGTGC
(CGCA)5 256–268 32 3 0.625 (0.446)
Takifugu fasciatus TF 117
s
KT768039
F:CCGCGTTCCTGTCTACTGTT
R:CTGAGCTCCGAACTTTGTCC
(CACG)5 251–259 32 5 0.594 (0.560)
Takifugu fasciatus TF 118
s
KT768040
F:GGCAAAGCACACCCAATAAT
R:TTCTCCGTCCATTCTCATCC
(AATC)5 226–234 32 2 0.250 (0.310)
Takifugu fasciatus TF 120
s
KT768041
F:GAAGCACCATCTGCATTTCA
R:TGTCATTATGGCCACAGCAG
(GTGC)5 266–283 32 6 0.156 (0.589)
Takifugu fasciatus TF 121
s
KT768042
F:AGCGAGCTGTCTGAAGGAAG
R:CCCCACCCCTTTTCTCTCTA
(AAAC)6 217–225 32 3 0.594 (0.599)
Takifugu fasciatus TF 123
s
KT768043
F:GCTGAGATCCACTGGGAGTC
R:GCCTTACGTATCTTCCAGCG
(ATTT)5 235–247 32 4 0.188 (0.348)
Takifugu fasciatus TF 124
s
KT768044
F:GAGGAAGCTGAGCAGACAGG
R:TGCATGCAGCTGTAAACACA
(TAAA)5 261–265 32 2 0.469 (0.504)
Takifugu fasciatus TF 126
s
KT768045
F:GTGAACGGTTTCAGTGGGAT
R:CAGGCCACCAAGGTACAGAT
(GAAA)5 261–265 32 2 0.406 (0.496)
Takifugu fasciatus TF 130
s
KT768046
F:GTCTGGGTCTGGCTGATTGT
R:TTGTGAGGAGCAGAGGTGTG
(CTGC)5 257–261 32 2 0.219 (0.365)
Takifugu fasciatus TF 132
s
KT768047
F:CTGAGACATTTGCTCACCGA
R:TGCCTGATGGCAACTAATGA
(TAAA)5 257–261 32 2 0.813 (0.490)
Takifugu fasciatus TF 136
s
KT768048
F:CGTCCATTGTCCTCATTGTG
R:AGACCCCCGCAAATAAAAAC
(TCAT)5 221–225 32 2 0.219 (0.329)
Takifugu fasciatus TF 139
s
KT768049
F:GAGGAGATGACAAAGCAGCC
R:GGTCTCACCGAGTGGTTGAT
(ATTT)5 294–298 32 2 0.188 (0.268)
Takifugu fasciatus TF 143
s
KT768050
F:TTGTAGCGCCTTGGAGAAGT
R:ATTCCTCAGCGGAGAACAGA
(GTTT)5 240–244 32 2 0.219 (0.329)
Takifugu fasciatus TF 144
s
KT768051
F:AGAAAATGAGCCCAGCTTGA
R:GGACAGTTTCTGCAGGCTTC
(AAGG)5 221–229 32 3 0.313 (0.372)
Takifugu fasciatus TF 165
s
KT768052
F:TGTGTGGGGAGGTGTATCCT
R:CGCGCACTGTACAACAGACT
(GT)7cctagtgtgtgtgtgggggct
gtatcctagagtgttgtaca
(GT)6tggtgtgtgtatcttag
gttgctgtacagtgtgttg
tac(TG)6
224–235 32 3 0.656 (0.585)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
95 96
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Takifugu fasciatus TF 166
s
KT768053
F:CCTGCCCATTCCTAACACTT
R:TGATATGTGAGAGACCCCCA
(AGAC)5ttgactgacatctgaaa
gcatctttcaggttatcatatgg
cttgcttaagccctgttgtaag
atatactaaaataataataacg
gtg(ATA)6
224–227 32 2 0.250 (0.222)
Takifugu fasciatus TF 169
s
KT768054
F:TTTTGTTTGAGGAGCAGCCT
R:CAAAGAGGAAGTGGTTTAACGG
(GCT)5gggccttatcccaaggggctga
catagctgacattcaacagataac
aaacacacacagacactcac
acacgt(AC)6
276–278 32 2 0.250 (0.222)
Takifugu fasciatus TF 158
s
KT768055
F:TTCCTAAAAGAGCCTTGCCA
R:CTTGCAGCAAAGCTGTAGCA
(AT)6(GT)6t(TA)8caca
catacatacatgta
taaatacacacatgtgtgtat
atgtatgtggat(AC)6
291–301 32 5 0.656 (0.665)
Takifugu fasciatus TF 172
s
KT768056
F:TCCTCCACAGGAGCTTGAAT
R:TGACGGATATGAGGAGGAGG
(CT)7ccgtctctg(TC)6 255–269 32 6 0.719 (0.715)
Takifugu fasciatus TF 173
s
KT768057
F:TTCAGAATGACAGCAGCAGG
R:CCCCTTCATCTAAGGTGCTG
(TAA)5tagtag(TAA)5 283–286 32 2 0.281 (0.329)
Takifugu fasciatus TF 184
s
KT768058
F:GGATCACGCAGATCACACAC
R:TGGGACTAATCCGCACTTTT
(CA)6cgt(AC)6 259–263 32 3 0.594 (0.573)
Takifugu fasciatus TF 186
s
KT768059
F:CAGACGGGTGAAGAGAGGAG
R:TCCTGGTCCCTCAGTTTCTG
(AC)8atacgcacg(CA)6 219–221 32 3 0.125 (0.404)
Hippocampus erectus Hier-SSR1
t
F:ATGGAAGAGTCAACATAAACC
R:TGAGTCACTACGGCAACC
(AC)
8
161–169 32 5 0.6667 (0.7606)
Hippocampus erectus Hier-SSR2
t
F:TCTGGGGCTCCGACTAAAC
R:GTAAACTGGAAACGCCTCAA
(ATT)
6
224–236 32 4 0.6667 (0.7289)
Hippocampus erectus Hier-SSR3
t
F:ACCTGCTACCAACCACAA
R:AACAAGGCAACCACTCATT
(GT)
8
271–285 32 5 0.6333 (0.8072)
Hippocampus erectus Hier-SSR4
t
F:GCACAACAAGAGGAGGGG
R:GGGCAAGAAGAGGCAGAG
(CTT)
6
170–185 32 4 0.5667 (0.7294)
Hippocampus erectus Hier-SSR5
t
F:GGTTTGGCAGTAGTGGGG
R:TTCGGTTTACGAGTAGAGCA
(TC)
7
289–299 32 5 0.5667 (0.7772)
Hippocampus erectus Hier-SSR6
t
F:CAGCGATGACGAGTGAGT
R:GCCAAAATGAAAACAGAGA
(AGA)
6
104–119 32 4 0.6000 (0.7383)
Hippocampus erectus Hier-SSR8
t
F:TGATGGTTGTGTGAGAAGGA
R:GAGAGAAAAAGACGGCGA
(AC)
8
129–141 32 7 0.7333 (0.8083)
Hippocampus erectus Hier-SSR9
t
F:GTTGATGCGACCCACGAT
R:GCCTTGCTCCTTCTCTACG
(TG)
6
220–242 32 4 0.5667 (0.7526)
Hippocampus erectus Hier-SSR10
t
F:CCAGTTAGCATTGCGTCT
R:TCTTAGCCAGCGAGTGTT
(CAA)
6
104–116 32 4 0.6333 (0.7343)
Hippocampus erectus Hier-SSR11
t
F:CGGGGTAGCAAGGTGAGC
R:CAAGGGGACATTGAAGTAGG
(GT)
6
279–295 32 5 0.6667 (0.6644)
Hippocampus erectus Hier-SSR12
t
F:AGTGGGTGTCTCTGTAAAC
R:CCTTCGCAGTATTCATTG
(TG)
6
223–235 32 7 0.7567 (0.7965)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
97 98
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Hippocampus erectus Hier-SSR13
t
F:TGCCATCATCGCTAACTAA
R:TGCCAAAGACACAAAAAGG
(CA)
6
206–222 32 4 0.5667 (0.7256)
Hippocampus erectus Hier-SSR16
t
F:TACATCAGCATTGACGCC
R:ATAAGTAGATAGCAGCCACG
(CA)
9
277–295 32 7 0.7258 (0.7865)
Hippocampus erectus Hier-SSR17
t
F:TGCCGAATGATGATACAC
R:AATGACGCAATGAGAACA
(CA)
8
230–246 32 4 0.5142 (0.7025)
Hippocampus erectus Hier-SSR18
t
F:TGGACTTTCTTTTCAGCACGA
R:GCTCAGCACCACGCACTC
(AAT)
6
216–231 32 4 0.6333 (0.7363)
Hippocampus erectus Hier-SSR19
t
F:CGGAAGCCACATCTAAACA
R:CATCCACATCCACCCACT
(CAC)
6
164–179 32 4 0.5869 (0.7005)
Hippocampus erectus Hier-SSR21
t
F:CTGGAAATCAACTTCAACACC
R:AGCCAATCCTCGTAGCCT
(ATT)
5
265–284 32 4 0.7568 (0.7867)
Hippocampus erectus Hier-SSR22
t
F:GAGGGTTATGAGCCTTTTG
R:TCGGTTGTTGCTATCTGC
(TGT)
6
262–287 32 5 0.8856 (0.821)
Hippocampus erectus Hier-SSR23
t
F:GCCACCGACACCATCCCT
R:TCTGCGGCTCGTCCTTCA
(GCA)
8
130–148 32 4 0.7562 (0.7122)
Hippocampus erectus Hier-SSR24
t
F:TGGAAATGTAAAAGGAATG
R:TAAAGGAGGTAGGCAATAA
(TGT)
8
268–284 32 5 0.862 (0.7567)
Hippocampus erectus Hier-SSR25
t
F:CCACCTGTGAACACTTGC
R:GCGTTGCTTTCCTCCTAC
(GT)
15
189–205 32 5 0.6556 (0.7255)
Hippocampus erectus Hier-SSR26
t
F:CAGGGCGACAACAAGATG
R:GTAATGGTGGGGAGGGAG
(GT)
15
240–262 32 6 0.8655 (0.7555)
Hippocampus erectus Hier-SSR27
t
F:CAGGAACACCAAATAGCC
R:CGAGTCAAGAGTCTGGAAAG
(GA)
18
201–219 32 6 0.7568 (0.6824)
Hippocampus erectus Hier-SSR28
t
F:GTGAGAAACGCACCAAAC
R:ACACGACAAAACAGCATC
(CA)
16
137–155 32 5 0.8521(0.7865)
Hippocampus erectus Hier-SSR29
t
F:TGCTCACAGGCTTCACAT
R:TCCTTTTCACAGTCCCAC
(CA)
10
203–223 32 4 0.7562 (0.6748)
Hippocampus erectus Hier-SSR30
t
F:TTTTGTTATCGCACTTTGT
R:AGCAGATTTGTAGGAGGG
(AC)
12
278–296 32 5 0.6667 (0.6878)
Lasionycteris noctivagans Lano1
u
F:
$
GGAAACAAGCTTAACATTTCAGG
R:AAAGACATGAAGAAGCCCTGC
AAAG 178–194 30 5 0.700 (0.709)
Lasionycteris noctivagans Lano3
u
F:
$
TCATATATAGGAGTATGTCTCTCGTGG
R:AATGAGCATGCTGGTTGGC
ATCT 237–265 30 7 0.833 (0.774)
Lasionycteris noctivagans Lano4
u
F:
$
GCTTTGGACAGTTTCACGGG
R:ACCAGATGGGAGCTCCTGC
ATCT 283–319 29 10 0.379* (0.825)
Lasionycteris noctivagans Lano8
u
F:
$
TCCATCTACCATCTACCTGCC
R:ATGGGTGGGTGTATGAAAGG
ATGG 242–290 29 11 0.793 (0.852)
Lasionycteris noctivagans Lano10
u
F:
$
GTGCAGCCATCTTGTAACGG
R:CAGCCCTAGCTGGTTTGACC
ATCT 300–356 30 14 0.467* (0.896)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
99 100
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Lasionycteris noctivagans Lano13
u
F:
$
AACTGATCATCTGGTCACTTAGGG
R:CCACTCTATGTACCCTCATAGCTGC
ATCT 271–395 30 14 0.900 (0.887)
Lasionycteris noctivagans Lano15
u
F:
$
GGGACAGCACATCCCTCC
R:CCCTAAGCACCACTGTCAGG
ATCT 196–308 27 8 0.741 (0.733)
Lasionycteris noctivagans Lano22
u
F:
$
TGGTATCCTGTCTCCTGGTCG
R:GATGCACCAGAGCCTCCC
ATCT 233–306 29 13 0.828 (0.834)
Lasionycteris noctivagans Lano26
u
F:
$
CCACACATCTACCCACTCATCC
R:GGCTCTTGGCTTACAGACTGG
ATGG 177–217 30 10 0.367* (0.796)
Lasionycteris noctivagans Lano28
u
F:
$
ATGCAAGTACGCAGTGACCC
R:GGGTGCATATATTGTTTCTGACAGG
AAAG 208–224 29 5 0.621 (0.683)
Lasionycteris noctivagans Lano29
u
F:
$
TGTAACCCTTGCAAGAAAGATGC
R:GGTCAAGGTCAACAGCCAGC
ATGG 200–240 30 11 0.733 (0.852)
Lasionycteris noctivagans Lano31
u
F:
$
ATTGGTTACCATTGCTGGC
R:TCCCTCTCTTTCTCACAGGG
ATCT 195–235 28 11 0.464* (0.854)
Lasionycteris noctivagans Lano32
u
F:
$
ACCCACATTTCTGAGTGACATGG
R:TCGAGCTTGACAAAGCCTACC
ATCT 218–262 29 10 0.414* (0.800)
Lasionycteris noctivagans Lano34
u
F:
$
GAGAGGCAGCACGGAAAGG
R:TGAATTTGTTGTTACTGATGTGAAGG
AAAG 195–235 30 11 0.733 (0.852)
Lasionycteris noctivagans Lano35
u
F:
$
ATCAGTCGGGCATCAAGAGG
R:TGAGAACCAGAGAGCCTGGG
AAAG 215–262 30 17 0.800 (0.891)
Lasionycteris noctivagans Lano40
u
F:
$
CCTTGAAACATTCATTTCTGGG
R:TGTCTTAGTTTGTGCCTTCCG
AAAG 223–255 30 13 0.900 (0.831)
Lasionycteris noctivagans Lano41
u
F:
$
ATCTGTCCAGTGTTGCTCCC
R:TGTGGTGCATTTCCATCC
AAAG 146–190 30 6 0.267* (0.794)
Lasionycteris noctivagans Lano44
u
F:
$
TCACAGTCAGATACCACTATGTACCC
R:TTGTAGTATGTCTCGATACTGCTTTCC
TTCC 284–334 29 14 0.621* (0.891)
Lasiurus cinereus Laci 1
u
F:
$
AGGGCTCAGTAAGCACCAGC
R:AAGCTAGCAGGAGCCAGAGC
TTCC 205–309 29 32 0.724* (0.944)
Lasiurus cinereus Laci 15
u
F:
$
TGAACAATCAGTAGGTATGTAAACTGC
R:CCATATGAAATTGCTGACAGTGC
ATCT 338–366 30 8 0.900 (0.828)
Lasiurus cinereus Laci 20
u
F:
$
GAGTGTTCCAATTAGAAGATAAGGC
R:CTCACCTGCTTCACAATCCC
ATCT 217–249 31 8 0.774 (0.766)
Lasiurus cinereus Laci 22
u
F:
$
CGCAGAAGGTAGCCAAGTAGG
R:GAAGGGCTATTCTCCATTCCC
AAAG 161–217 31 12 0.839 (0.844)
Lasiurus cinereus Laci 25
u
F:
$
TTGCAGATGGCAGATCTTGG
R:AAATATATGAGAGCAAGTGTATTAGCC
ATCT 204–224 31 6 0.290* (0.683)
Lasiurus cinereus Laci 26
u
F:
$
CTTGACATAGACTCATGTTTCACTCC
R:GCACACATAGAGTCCGATGAGG
TTCC 271–321 30 18 0.933 (0.894)
Lasiurus cinereus Laci 28
u
F:
$
TTCTGTTATTTGTCCTGTGAATTGC
R:GTCCGGGTCTGTGAGAGGG
ATATCT 294–342 30 10 0.733* (0.859)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
101 102
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Lasiurus cinereus Laci 29
u
F:
$
GTGTGCCCTTGAGGAACAGC
R:GTCTCTGTGAGCAGAGGAGGG
ATCT 197–251 31 12 0.742* (0.883)
Lasiurus cinereus Laci 31
u
F:
$
GAGCATATGTCAGGAAGGAGG
R:TATGCTCTTGAGGAGCTTGC
AAGAG 309–387 31 13 0.774 (0.872)
Lasiurus cinereus Laci 33
u
F:
$
TGACTAGGGCTGGTTGAGC
R:CAGAATATATTTGTGTACAGAACTGGG
AATAAG 279–379 31 17 0.871 (0.895)
Lasiurus cinereus Laci 34
u
F:
$
GGCTTTCTAACCCTGGGACC
R:TCAGGATTCTTTAGAGAAACAGAAGC
ATCT 259–299 31 13 0.935 (0.894)
Lasiurus cinereus Laci 35
u
F:
$
CAGTTGATTGAACTTCCAAGGG
R:TCATCAGAAGTCCTGGTTGGG
ATATCT 211–337 31 31 0.968 (0.949)
Lasiurus cinereus Laci 36
u
F:
$
CCTTTCTCTGTCCCTCAGTGC
R:TCCACCTCCCACCATTTCC
AAAG 175–230 31 13 0.871 (0.853)
Lasiurus cinereus Laci 37
u
F:
$
CACGACTTCCTGGTACTTCTCG
R:TCTCCTGTTCTTTGCATCCTAGC
TTCC 244–349 27 18 0.222* (0.911)
Lasiurus cinereus Laci 38
u
F:
$
TCACCTAATATGCAGCTCTGGG
R:TGAAAGAGTAACATACATCCGACCC
AAAG 266–342 30 22 0.533* (0.923)
Lasiurus cinereus Laci 39
u
F:
$
GCTCTTGTTGATGTTTCTCTTTCTCC
R:TGATGTTTCAATTGCATGATTCC
AAAG 283–347 31 14 0.935 (0.866)
Lasiurus cinereus Laci 41
u
F:
$
CCAATACAGGCAGCATTAGCC
R:TGCAGTTGGTAATTACTGGAGGG
AAAG 197–321 30 17 0.767 (0.885)
Lasiurus cinereus Laci 42
u
F:
$
TGTTTACCATGTCATCCAGGG
R:TGCATGTGGTATCCCTCCC
TTCC 182–234 31 10 0.516* (0.823)
Lasiurus cinereus Laci 43
u
F:
$
GACCGCTTTGTAAAGTATGTGGG
R:TGCCTGTGGCCTTTATGACC
TTCC 172–224 31 10 0.645 (0.850)
Lasiurus cinereus Laci 48
u
F:
$
TTCCACAAATGCTGAAAGGG
R:TTTCTCTGCGATGGAAGAACC
AAAT 177–217 31 12 0.645 (0.843)
Saurogobio dabryi sdF5010
v
F:GCTCCATTCCCAACCTATAACACA
R:TGCCTTGATGAACGGTTATGTATG
ACAT 78–109 24 10 0.62500* (0.85727)
Saurogobio dabryi sdF5117
v
F:CGAGTAGACTTATGCGTTTCAATGG
R:GAATTTCCCCCACTGCTAATACAA
AAT 134–153 27 8 0.70370 (0.81272)
Saurogobio dabryi sdF5129
v
F:CGAGTAGACTTATGCGTTTCAATGG
R:GAATTTCCCCCACTGCTAATACAA
TC 123–158 27 19 0.74074* (0.92872)
Saurogobio dabryi sdF5145
v
F:AACTGTAGGGCACGACAAATTGAT
R:AGTCTAAACCCGTCTGCAAGAATG
AGAC 188–260 27 18 0.85185 (0.93361)
Saurogobio dabryi sdF5163
v
F:TCTCAGATGACGTTGAGCATATTGA
R:CATTCATCTGGGCTCACTAAAACA
ATCT 158–210 26 15 0.80769 (0.93361)
Saurogobio dabryi sdF5180
v
F:TGTGGGTCTGAGATTTGTGATGTT
R:GGCCCATGCGTAAGTAGGTTATC
AG 127–143 27 9 0.74074 (0.80643)
Saurogobio dabryi sdF5226
v
F:AGACTCGGCTGCACCATCTATTAC
R:AGCAATTTGGGAGATTTGTGATGT
AGTT 113–151 20 10 0.55000* (0.85513)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
103 104
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Saurogobio dabryi sdF5234
v
F:TTTGAGACTGGCTCAGATGTTCAG
R:CAGAGTGGAAATCTGGGTAAATGG
GCT 158–168 27 8 0.66667* (0.83438)
Saurogobio dabryi sdF5257
v
F:TGAGGAAGAGGAGGAGACTTCAGA
R:CTCTATGAGCTCAGATGGGGATTC
GAG 125–134 27 4 0.25926* (0.41090)
Saurogobio dabryi sdF5261
v
F:CACCAGAGCCACATATAAGCACAC
R:ACGAAGACAGGACTCCAGAGAAGA
TCG 121–129 27 6 0.29630* (0.53529)
Saurogobio dabryi sdF5271
v
F:ATGACAAAGGAAAACCACGAGAAA
R:TGCAGATACAGCGCATCACTTTAG
AAAC 114–145 26 16 0.80769 (0.87406)
Saurogobio dabryi sdF5297
v
F:CGAGTAGACGGAGTTCAGCACAT
R:TGTTGTATAAGCGTGTGATGTGGA
AC 122–124 27 2 0.74074* (0.47519)
Saurogobio dabryi sdF5302
v
F:GCATGTTGACCTGCAAGATAGAAA
R:AACATAAATGAAGCCTTGGTGAGC
TA 118–131 27 3 0.14815 (0.14256)
Saurogobio dabryi sdF5318
v
F:CGCTGGATATTGAAAGAGTCTGGT
R:CTTGTCCTCCTAATCCTCCTCCTC
GGA 160–172 27 9 0.62963* (0.77289)
Saurogobio dabryi sdF5328
v
F:GTACGTGGTGTATTGCCAGAACAA
R:CCAAAACAGTCCCTCATGCTTAAT
AC 231–261 26 11 0.46154 (0.54148)
Saurogobio dabryi sdF5336
v
F:GGTGGTGATTAGTAGGCTGGTGAC
R:CTGACTGCCCCCAATTACAGAATA
TAT 108–116 27 4 0.25926* (0.64640)
Saurogobio dabryi sdF5340
v
F:AAAAGACGCCCACACTTTTATCTG
R:TCACAATCCACATTTTCCTTAAACC
CTTT 115–333 27 6 0.14815* (0.69672)
Saurogobio dabryi sdF5351
v
F:CGAGTAGACTAGACGCCCACACTT
R:TTCACAATCCACATTTCCTTAAACC
CTTT 126–210 26 20 0.84615 (0.81146)
Saurogobio dabryi sdF5395
v
F:TTTCTGTACTTGTTAGTTTGGGGTCA
R:GATCAATTAAATGCATCATTGCTGA
TTTC 100–109 27 6 0.37037* (0.64710)
Saurogobio dabryi sdF5432
v
F:AAGAAGAGGAAGGAGAAGGCGAG
R:CGGTTTCCTCCTGTTTTTCTCAGT
GAG 74–99 27 10 0.81481 (0.85255)
Saurogobio dabryi sdF5439
v
F:TTCGGTTTTTACCGTGATCTCAAT
R:GATAACACTGACTGACTGCTGGGA
GAG 139–147 26 5 0.34615* (0.74434)
Saurogobio dabryi sdF5466
v
F:CAGAGCCACATATAAGCACACCAC
R:CGGTTGTACGAAGACAGACTCAGA
TCG 135–153 26 7 0.34615 (0.40573)
Saurogobio dabryi sdF5484
v
F:TTTCCGCAGGCAGAGATAGTAAAG
R:GAGCAGAGTGGCGTTAGAGGG
AC 135–170 25 17 0.72000 (0.90857)
Saurogobio dabryi sdF5506
v
F:TCAAATGTATTTCAGCCGATGCTA
R:ATGAGGGTGTTTGTGTATGTGGGT
ACT 131–147 27 6 0.51852 (0.61635)
Saurogobio dabryi sdF5513
v
F:TAAGCACACCAGTCCCATTCACTA
R:ACAGACTCAGGACTGGAGACGAAG
ATC 94–167 26 9 0.61538 (0.69457)
Saurogobio dabryi sdF6119
v
F:TCATGCAAACAAGAGTTGAGCAAT
R:GGCTCTCAAAATTCTCCCAAGAAG
TGATT 76–93 27 6 0.70370 (0.72607)
Saurogobio dabryi sdF6273
v
F:CACAGACGAGGATTCCAGTCG
R:ACATGCAGTCCCTTACCTGTTCTC
GAG 156–174 27 5 0.81481 (0.76380)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
105 106
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Saurogobio dabryi sdF6293
v
F:CGTCTAGTTTTGTCGCTCTGTTGA
R:TGCTTACTTGAGGTATGCTGGTTG
CATA 78–128 27 8 0.29630* (0.77358)
Saurogobio dabryi sdF6322
v
F:CTTAACTAAACCGCACCCCTCTCT
R:GTCTCCTCTCCTCTGAGCTGACTG
TCC 148–149 27 2 0.00000* (0.07268)
Saurogobio dabryi sdF6324
v
F:TGTAGCGTTCCAAACCTTTCTCAT
R:GGGAGTATGGTGGGGAAGTCTTAT
CCT 136–137 25 2 0.00000* (0.07837)
Saurogobio dabryi sdF6400
v
F:GTGGCTCCTTCCCTTTCACAG
R:AGCAGATTGTACATGTGGCTCAAG
GGA 101–131 26 9 0.80769 (0.83786)
Saurogobio dabryi sdF6432
v
F:TTCTGGACATTCCACCTAAAACATC
R:TCATAGAGGAAACAATGTGTAATCCAA
CTATT 135–198 26 17 0.88462 (0.92534)
Saurogobio dabryi sdF6476
v
F:CGTCTAGTGCTGAAGGAGGTGAGT
R:TCTCAGCCTGGAACACAGAGAGAT
ACTC 144–268 26 25 0.84615 (0.94947)
Saurogobio dabryi sdF6479
v
F:TCCGTTGTTTAGGCTACTGATCAAA
R:TGAGATGACATGACGATAGCTGTG
GATA 166–227 25 17 0.80000* (0.93959)
Saurogobio dabryi sdF7038
v
F:GGCAAGCTGTTTGACTTATTCATTC
R:TTTAACCCACTGGACCACCATATC
AGT 122–450 27 5 0.07407* (0.64011)
Saurogobio dabryi sdF7068
v
F:TTAAAGCAGGGTTCACATCCAC
R:ACGTGTGACATTTTTATCTAGGAATACA
AC 98–102 24 4 0.16667 (0.16046)
Saurogobio dabryi sdF7209
v
F:ACACTACTCGTCTGCCGCAA
R:ACAATGCAGATTGTTTCAAAGCAG
AAAT 98–102 27 2 1.00000* (0.50943)
Saurogobio dabryi sdF7235
v
F:ATCTTTCTCAAAATCTGGCAGGTG
R:AGTTGCTGTAGTGACCCAAAGAGC
TCA 129–213 27 4 0.22222 (0.20894)
Saurogobio dabryi sdF8049
v
F:AAAACACACAAGCCAGAGAGACAG
R:AGGTTGTTTGGTGTAAACGACGAT
ATC 75–94 20 4 0.30000 (0.35000)
Saurogobio dabryi sdF8133
v
F:GTATACCCCCATGGGAAGAAGAAG
R:GGGGGACTACTTTGTAAAAATGGC
AAT 116–131 27 5 0.81481* (0.68134)
Tetrapedia diversipes T1
w
F:ACTGCATACGTGCACGGTGGT
R:GCAGCGAACGAGTCGGAGCA
(GA)
12
257–293 22 6 0.545 (0.647)
Tetrapedia diversipes T2
w
F:CGGCGATACCGTAGCGGGAG
R:GCACGCTCTTCAATCGCGCC
(AG)
5
A
3
G
3
(AGA)
3
(GA)
4
192–198 22 5 0.500 (0.718)
Tetrapedia diversipes T3
w
F:GCACCCTCCTCCGTTCGTGC
R:GCCTCCGAGGTGGCTCGTCT
(GAA)
6
175–181 22 4 0.682 (0.681)
Tetrapedia diversipes T4
w
F:CCCGCGGGTTGCTGGCTAA
R:CCATGGCCCGAACAGGCGAA
(GA)
3
CA(GA)
4
G
2
(GA)
9
181–189 22 4 0.455 (0.680)
Tetrapedia diversipes T5
w
F:TGGGGTTCGATAACGGAGCGGA
R:GGGAGGGCCAGATCGAGCCTA
(AG)
3
TG(AG)
10
202–220 22 9 0.909 (0.816)
Tetrapedia diversipes T6
w
F:TGTAACAAACGATCGGAAGCGGGA
R:CGGTTGTTCGTTATACGTGAGTGTCCG
(CT)
12
223–239 20 7 0.650 (0.640)
Tetrapedia diversipes T7
w
F:ATAACGGCCGCACGCGGAAA
R:TTGTAACGCGGCGGTGAACG
(AG)
3
G
3
(AG)
6
A
3
G
2
(AGA)
2
(AG)
4
200–216 22 9 0.727 (0.853)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
107 108
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Tetrapedia diversipes T9
w
F:CCGGTGTGCGCGAAACAGAGA
R:AGCTCCTTCGGACCCGGCAA
(AG)
3
A
2
(AG)
12
215–255 22 17 0.864 (0.915)
Tetrapedia diversipes T10
w
F:CGCGAGAATTTGGGACCATCGTG
R:CCGCGACGACCCCTGCTCTA
(AG)
10
250–256 22 4 0.455 (0.580)
Tetrapedia diversipes T11
w
F:TCCTGGATCGGGCTGTGCGA
R:CGTTTCAAAGTGTCCGCACAAAACA
(AG)
7
A
2
G
2
T(GA)
2
A(AG)
7
201–263 22 12 0.864 (0.880)
Tetrapedia diversipes T12
w
F:TTCGGGACACGTTCGACGGC
R:TCTGTTCTCCGCGAGGGCGA
(AG)9 179–225 22 12 0.818 (0.860)
Tetrapedia diversipes T13
w
F:TGCACGAAGCAGCCGCCTTT
R:CCCGAGGATCGGGGCAGGAA
(AG)2A2(AG)6A2(AG)2(AG)9 102–128 21 10 0.667 (0.805)
Tetrapedia diversipes T14
w
F:GCTTTAAGCTGTGAATACTGCTCTGGA
R:GGTCCGGGTGTTGCTAGGGC
(AG)15 221–255 18 10 0.278* (0.832)
Tetrapedia diversipes T15
w
F:CGAGTGACCGGCAACGTAGCAAA
R:TTCGTTGCCGCTGTGCAAGT
(CT)8T4(CT)4 139–171 22 16 0.864 (0.866)
Tetrapedia diversipes T16
w
F:TGCGTCGGAACTGCTTTCAGATT
R:CCGGTGTCTCGTAAACGCCGTC
(TC)2T2(TC)13TGTCTG(TC)3 162–188 22 14 0.909 (0.894)
Tetrapedia diversipes T17_2
w
F:CGAGGTTTCGTTTACGTCCAG
R:GTTTGGACGATTACACGCGG
(CT)11 219–257 22 13 0.909 (0.862)
Tetrapedia diversipes T18
w
F:GCTACTACTACTAACCTTCGAGCCCA
R:CGTTGGTTCGCTTTGCACGGG
(AG)14 256–288 22 14 0.636 (0.877)
Tetrapedia diversipes T20
w
F:GCAGGCCACGGCCGGTAAAT
R:CGGTAAGCGCGAGCATCGACA
(CT)13 246–267 21 8 0.762 (0.783)
Tetrapedia diversipes T23
w
F:CAAAATCGATTCCCCGTGGC
R:AGTTCGGGCAACTTTCGAT
(AG)11 207–227 22 9 0.818 (0.856)
Tetrapedia diversipes T24
w
F:ACACCCACCGAGATTACGGCCT
R:AAAACCGTGTGCGCCGTTGG
(GA)22 251–287 22 8 0.545 (0.655)
Tetrapedia diversipes T25
w
F:TGTTCACTGTGTATGCGTGT
R:GGATCGATCCAGGTCGATCTG
(GA)5A2GAC(AG)13 192–228 21 16 0.810 (0.902)
Solen grandis DZC-2
x
F:CCAAAAATGGTTTTAGTCATGTTGC
R:TTCAGATTTGTCATGGAACGATTG
(GT)
11
136–172 30 13 0.8333 (0.9158)
Solen grandis DZC-9
x
F:ACGAAAATCAAGTAATGATCCCAA
R:TGGTGATGATAAGGAATAAGATGATG
(ATC)
12
126–175 30 11 0.6333 (0.7825)
Solen grandis DZC-15
x
F:AATTGCTAAATCATGTTGATACTGATGA
R:AAATTCTTTCACATTTTGGGGAGA
(TC)
14
68–114 30 13 0.6333* (0.7627)
Solen grandis DZC-22
x
F:GGGTGGGTCTGTGGTTATGTCTT
R:AATGGACGATAAAGTAGCGATGGA
(CT)
11
76–120 30 15 1.0000 (0.8938)
Solen grandis DZC-19
x
F:TTTTTCTTGTTTTCACCATCCATAA
R:ATTACATAACCAGTGACCTTGGCA
(GA)
13
126–154 30 11 1.0000* (0.8831)
Solen grandis DZC-28
x
F:AAAGCAATGTCAATTTCCGTGTTT
R:AGCGAAAGTTCACGACATAGATCC
(TG)
8
128–200 30 22 1.0000* (0.9475)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
109 110
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Solen grandis DZC-31
x
F:CTCAGACCAAGCGAGATGTTTTG
R:ACGCTTCCTTTTGCAACTGTTTAT
(CA)
17
102–130 30 14 1.0000* (0.9085)
Solen grandis DZC-16
x
F:AGTCAACATAAAGCATTTACAATTATGC
R:GCGATTTGTATCGATATGTGTCTAAA
(CT)
20
124–160 30 14 1.0000 (0.9147)
Solen grandis DZC-36
x
F:TATGATATTGGCGACAGAAGGATG
R:TATTTTCGGGGAAAGAGACAGACA
(AC)
19
112–146 30 15 0.4667 (0.9079)
Solen grandis DZC-96
x
F:GGTGGCAGAGGCACTAAAATAAAA
R:TCCCACCTCTCACCTGTTCATAAT
(TGA)
6
152–164 30 7 0.9333 (0.6751)
Solen grandis DZC-98
x
F:CATCTTTTTCATACATTACAGGGGTGA
R:GTGAGTGTCTTTAGGGCCATCTTG
(TA)
6
130–162 30 15 1.0000 (0.8164)
Solen grandis DZC-82
x
F:ACGCCTAGGGAGTAACTAAGGATTTC
R:ACGTTTGCGACTGTAACAATGAGA
(GT)
19
144–190 30 19 0.6667 (0.9367)
Solen grandis DZC-87
x
F:CTCATTGTTACAGTCGCAAACGTC
R:GTAGCATGTGATTTGTCGTCCTTG
(ATT)
13
120–160 30 9 0.8333 (0.5887)
Solen grandis DZC-66
x
F:ATTTCCAAGGCATGAAAAGACAAA
R:ATTTCCAAGGCATGAAAAGACAAA
(TC)
14
130–158 30 14 0.8667 (0.8288)
Solen grandis DZC-102
x
F:AGTTGTATGTGTCGTCTGCGGTAA
R:CCCTTGTACCAATGAATAACGAGG
(TTG)
5
146–180 30 12 0.8333 (0.8243)
Solen grandis DZC-131
x
F:GGCCTTCACTCGATTCTATCACAT
R:GTGACTTCACGCTCTGGTAAGGTT
(AGAA)
8
130–154 30 9 0.9667 (0.6763)
Solen grandis DZC-136
x
F:AAACCACACCACAATGCACAATAC
R:AGCTTTCAGACCTGGGTTTTCTCT
(AAC)
5
130–143 30 7 0.8333 (0.7011)
Solen grandis DZC-139
x
F:CAGCAATAGGAAAAAGCCCTAACA
R:TGGAATTCAAAAGTAAGTGTCCGA
(GAT)
13
117–150 30 13 0.8333 (0.8266)
Solen grandis DZC-149
x
F: TCATAACTGCATCGTATTGTTGGC
R: GCTGAACGTCGCTATATGACTGAA
(AATG)
4
146–170 30 11 0.7667 (0.8147)
Solen grandis DZC-113
x
F: AACACAATGTCTCTGTGCCTGTGT
R: AACACTCACTCCCACTCAATCTCA
(GA)
8
124–134 30 6 0.6333 (0.7136)
Solen grandis DZC-70
x
F: TTTGACATTGGTTTTTAGGCTGTT
R: CGTCGTTGCACTGAAAAATAAAAT
(TC)
9
146–180 30 11 0.9333 (0.5915)
Solen grandis DZC-78
x
F: CTGGTAAGCTGTAAGCGATGATGA
R: TTTTGGTTTGTCAGTAGGTTTCCAA
(ATG)
11
133–167 30 15 0.9667 (0.8390)
Solen grandis DZC-77
x
F: TGCATTGAATTTTGAGGTAGGTAGG
R: TCGGATACGGTACAATCGTAAGTG
(CA)
12
130–194 30 16 0.9000 (0.7989)
Solen grandis DZC-86
x
F: AAATCACATTCAAAGAGTGCCTCC
R: GCTCGCAGAAATAAAAGCTGCTAA
(ATG)
7
144–160 30 7 1.0000 (0.5927)
Solen grandis DZC-57
x
F: TGCTTACAGCGCTTTTATTTCTCA
R: TGTTTTCATGTTTTATTTCGTACCCA
(AG)
16
134–168 30 13 1.0000 (0.8503)
Solen grandis DZC-93
x
F: TGTGCAGTTAATTGTTGTCAGTGC
R: TAATTAGAGGAAAGCCCTGTCGTG
(AT)
9
160–200 30 11 1.0000 (0.7203)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
111 112
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Solen grandis DZC-92
x
F: TTTGAACGTTCTCTTTAGTTGGTCG
R: GGGCATACTCCACACATTTCTTTT
(TG)
10
126–156 30 8 0.9667 (0.7062)
Solen grandis DZC-88
x
F: AGATATCATGAAAACGGGATCTGG
R:ACAAGGTTGCACTTATGTCTGTATTCTT
(TA)
6
120–158 30 18 0.9333 (0.9260)
Solen grandis DZC-73
x
F: CTTTTGTTCAAATGGACGGCTTAC
R: TTCTGTGTGTTCCCTTTTGGTTCT
(CT)
20
130–164 30 16 0.9667 (0.9186)
Solen grandis DZC-72
x
F: ATAGCGCCATGTTAGACCTTTGAA
R: CCCTGTGAGGTACTCGTTTGCTAT
(GT)
20
122–160 30 19 0.933 (0.9412)
Solen grandis DZC-64
x
F:GATAAATGTACTTGCATTACTTCAGGC
R: GTAGCACTTTGTCTAATGATGGCA
(TG)
7
140–170 30 13 0.8333 (0.8842)
Solen grandis DZC-56
x
F: AGACGGACAGTCTTACCACTTTGTT
R: GGGAGTGTGGAAGGACAGTAACTC
(AC)
10
154–204 30 15 0.9000 (0.9045)
Solen grandis DZC-99
x
F: TGTGAAAACATTGCAAGGTAGGAT
R: TTCAGCAAAACAAGAAAACAATGA
(CA)
16
150–184 30 15 0.5000 (0.8525)
Solen grandis DZC-89
x
F: TGTGTTGCCTAACACGTCTTGATT
R: TCATAAAATCTGCTGCAGTTACGC
(GA)
6
104–130 30 14 0.9667 (0.8588)
Solen grandis DZC-58
x
F: TATGACTGAAATACTGTCGAGGCG
R: TAAAACAACATTTGTTGCCTCCCT
(AGTCA)
6
124–148 30 10 0.8000 (0.5616)
Solen grandis DZC-55
x
F: ATCGGATGTGGTCATTTTGATTG
R: TGGTTTAAAATGGTATGAGCGTATTG
(GT)
16
140–186 30 20 0.7000 (0.9362)
Solen grandis DZC-18
x
F: TTAAGTTGTTGCCTTGAGTAGCCC
R: TTTCATCCGATAAGGAGATTCGAG
(GT)
9
114–126 30 7 0.9667 (0.6452)
Solen grandis DZC-137
x
F: GTAGTGTCCTTTCGGCTTATCACC
R: TCCGGATCAGTAACTCAAAACCAT
(CATA)
5
102–114 30 6 1.0000 (0.6079)
Solen grandis DZC-147
x
F:GCACACGAGTACATATTATGGGATCA
R: TCGCTTTAAGGGAATAGGTAAGGC
(TC)
15
92–118 30 12 0.8333 (0.8785)
Solen grandis DZC-142
x
F: GTGCGATTGCAGATTGTTCTGT
R: TGCAATCGACGTGAAATAAACAAG
(ACAG)
4
154–174 30 11 0.7333 (0.8893)
Solen grandis DZC-97
x
F: CAAATGTCTAGCCTTCTGACACCA
R: ATTCTCTTTCTCTCCCTCTCTCCC
(AG)
6
100–128 30 13 0.8333 (0.8588)
Solen grandis DZC-23
x
F: GGGTGGGTCTGTGGTTATGTCTT
R: AATGGACGATAAAGTAGCGATGGA
(GT)
9
82–128 30 14 0.7333 (0.8470)
Solen grandis DZC-25
x
F: GCCGGAAATAGGGTATGATGTTTT
R: CCAGTACCATTTAAAAGCCACCAC
(TGA)
18
100–164 30 16 0.7667 (0.9147)
Solen grandis DZC-20
x
F: TTCAAATTTACAACCCAACCCAAC
R: AATCTCCTTCCGTTGAAGAAATCC
(AG)
13
96–124 30 13 0.7333 (0.9192)
Solen grandis DZC-24
x
F: GCCGGAAATAGGGTATGATGTTTT
R: CCAGTACCATTTAAAAGCCACCAC
(GTT)
8
83–152 30 14 0.8333 (0.9023)
Solen grandis DZC-45
x
F:GGGTGACAAGTGACAACAATAGAGC
R: CAAAATCAAATTGTAGCCCAGGTC
(ATA)
6
130–160 30 9 0.7667 (0.8090)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
113 114
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Solen grandis DZC-1
x
F: TTGCGACGGAGTGGTAATTTCATA
R: AAAATTGATTGTGCGGCCTTTTT
(CT)
10
128–146 30 10 0.9667 (0.8751)
Solen grandis DZC-46
x
F: CGTATTTCTTCTTCATTTCGTCCG
R: CCCTTTGCTCTTCTCCTTGTGATA
(TGAT)
9
96–172 30 13 0.7667 (0.8915)
Solen grandis DZC-3
x
F: TTTAAAACCGACATCATCTTGCAT
R: ATCCTCTTCTCCTCCTTTTTCCAA
(GA)
12
78–100 30 12 1.0000 (0.9119)
Solen grandis DZC-5
x
F: TTGGCTGCATGTTGATTTTATTTG
R: AAATCATTTATGCACTGCACATTCA
(TA)
6
148–160 30 7 0.9333 (0.8169)
Boehmeria longispica N1
y
F:AATTTCAAAAGCGTTGAGCG
R:TTGTTCTCTTTTTCCACGGC
(T)17 163 30 3 0.82 (0.66)
Boehmeria longispica N2
y
F:CGTCGAGTTTCTATGGAGCG
R:GGGATAAACAAAGTAAGCAAGCA
(T)17 109 30 2 0.00* (0.51)
Boehmeria longispica N3
y
F:ACTGATCCAGCTTCCTTCAG
R:CCACACAAGGCGGGTAATAA
(A)15 166 30 2 0.46 (0.50)
Boehmeria longispica N4
y
F:CCCAATTTAGTTGTGTCTAATTCTCA
R:TTTAGTTTTGCGTTTGGGTT
(T)16 104 30 2 0.47 (0.44)
Boehmeria longispica N5
y
F:TTTCTGCCACAACAGGCATA
R:CTTTTGCGTGATGGGTTTTT
(A)15 125 30 2 0.39 (0.39)
Boehmeria longispica N6
y
F:AGACCCTCCGTTTCAGAGGT
R:CACCGCAGCAAAACTCTACA
(AG)10 106 30 2 0.29 (0.45)
Boehmeria longispica N7
y
F:TGGTTTAGTTACCAAAATGCCC
R:TTCTCTTTGGTCGTGTTCCA
(AC)8 113 30 2 0.00* (0.31)
Boehmeria longispica N8
y
F:ATCCTAGGAGCCAAAAACCG
R:CCTCTCTCTATCTCCTTGGGC
(AG)13 140 30 3 0.47 (0.68)
Boehmeria longispica N9
y
F:CTTCGATACCTCCAAGTCGC
R:CCTAACCCTCGGGAAGAAAA
(CT)8 136 30 3 0.52 (0.61)
Boehmeria longispica N10
y
F:TTCTCACCGTTAATCTCGGC
R:AATCTCTGGACTAATATTCTGTGATTC
(TC)9 113 30 2 0.31 (0.51)
Boehmeria longispica N11
y
F:GGATTCATTTATGGGTGCCTT
R:CCCGTAGTTGTGATTGTGACC
(CAA)6 130 30 2 0.53 (0.40)
Boehmeria longispica N12
y
F:CCCCTTTTGCAGTTGTTGTT
R:TGTCAAGGAGGACAAGGCTC
(TTG)14 116 30 2 0.11* (0.50)
Boehmeria longispica N13
y
F:GCATACATAGCCCACGGATT
R:TCCAAGCATACCAAGCATCTC
(GGA)6 112 30 2 0.05* (0.50)
Boehmeria longispica N14
y
F:CATTGGATAGACCAAAATCGC
R:CAAATCTCAATTATACCCCTCCTT
(GAA)8 103 30 2 0.42 (0.44)
Boehmeria longispica N15
y
F:CTCCCAATCATTGCCAAATC
R:TATCGCTTCTTGGGCTTCAT
(AGA)5 110 30 3 0.20 (0.19)
Boehmeria longispica N16
y
F:ACCTCACAGTGGAGGCTTGT
R:CAGCCTATACGCAAAGGTCA
(ATTA)4 142 30 2 0.00 * (0.37)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
115 116
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Boehmeria longispica N17
y
F:CCAACCAGCTTTTGAAGGTC
R:CCAGAGGTATAATTAGTTGTGCTAGTT
(AAAT)4 139 30 2 0.23 (0.49)
Boehmeria longispica N18
y
F:CTTTTTCAATGGAGGAGTTCG
R:CACACGCAAGACATAGTATTGGA
(TAAA)4 114 30 3 0.21* (0.61)
Boehmeria longispica N19
y
F:ACATATCGAGAATCGGCGAC
R:AAAATGTCGACAGGGTTTGT
(AAAT)4 127 30 2 0.36 (0.46)
Boehmeria longispica N20
y
F:CGAATCCATTTGACATCTCTG
R:CGAATGTCGAATCCTAAAGCA
(TATT)5 148 30 3 0.47 (0.66)
Boehmeria longispica N21
y
F:TGAGAGAAAAAGAAAGAAAAATTTGA
R:GTGGGAAAAGCCCTTGATTT
(AAGGA)3 151 30 2 0.29(0.33)
Boehmeria longispica N22
y
F:GAATCGAGAGATTATCGGCG
R:CATCAAATGACACATCAATCCC
(AGTGG)3 103 30 3 0.40 (0.62)
Boehmeria longispica N23
y
F:CACGGTTTTCCAAAAATCAAA
R:GCTTTCTAAGCCATGATTTTCA
(ATTTA)3 156 30 3 0.43 (0.64)
Boehmeria longispica N24
y
F:TCCAACCAAGGAGGAGAATTT
R:AACATTTCTAAGCCATGATTTTCA
(ATTTA)3 145 30 2 0.37 (0.48)
Boehmeria longispica N25
y
F:TAGGGCTTGGATTATGCTCG
R:TCCGTTCGAGATTCTATCGC
(TCTCT)3 151 30 2 0.57 (0.50)
Boehmeria longispica N26
y
F:AAGCTGAACGCCGAGATG
R:CCCACACGAGTTTCACCTCT
(GAGTCG)3 163 30 2 0.23 (0.42)
Boehmeria longispica N27
y
F:CTGAAGCTGAAAGCCGAGAT
R:TCACCTTCACTCACGCTCAC
(TCGGAG)3 117 30 3 0.62 (0.49)
Boehmeria longispica N28
y
F:TGTTTATCTTTCCACCCCCT
R:ATACTTGGACCGTTTGAGCC
(ATTTTT)3 141 30 2 0.64 (0.45)
Boehmeria longispica N29
y
F:AGGCGTTTCAGAGGATCAAC
R:GCGTAGCCTTCTTCTCCTCC
(CATTGT)3 151 30 3 0.14 (0.36)
Boehmeria longispica N30
y
F:CATCAATGGTCACTCACGCT
R:AGAAGCTGAAAGCCGAGATG
(CTCCGA)3 126 30 2 0.41 (0.33)
Exopalaemon carinicauda EC01
z
F:TGAACACTATTGGGAAACCTG
R:CTCATGCTTTAAGACGAACGT
(ATT)6…(TTA)6…(TTA)5…(TTG)5 353–404 30 4 0.8000 (0.5514)
Exopalaemon carinicauda EC02
z
F:TTTTGGTGTCTCCGTGCCT
R:CCAGGCGAACTACGACAGAT
(AC)14 240–353 30 7 0.5862 (0.7592)
Exopalaemon carinicauda EC03
z
F:GAGGTTGGTCGGTCTGTTTAT
R:ATTCGTTGGCATCCTTCAG
(AG)16 89–110 30 4 0.0667 (0.1881)
Exopalaemon carinicauda EC04
z
F:GAATAGAGGAGGTGGCTGAG
R:CACTTTCTGAGGAGTAGGGAG
(AGT)16 250–353 30 8 0.6897 (0.7169)
Exopalaemon carinicauda EC05
z
F:TCCGTAGTTAGGACGCTTGTT
R:TTTATTCAGCCTTAGCCATCAG
(CTT)12 242–343 30 8 0.6667 (0.8395)
Exopalaemon carinicauda EC06
z
F:GTCCAAACTCCGAGTGAAAAT
R:ATCGCAACACCGACAGAC
(CTT)23 190–239 30 8 0.7667 (0.8452)
Exopalaemon carinicauda EC07
z
F:TGATCAGGGGTAGATGGTTT
R:GGCAAAAGGGATTTCTAATG
(GAT)10 242–353 30 8 0.6667 (0.8542)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
117 118
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Exopalaemon carinicauda EC08
z
F:ACACAAGGGCCAACAGTCT
R:GCCGTGATTCCTCCTTTT
(TCA)10 242–353 30 6 0.7241 (0.5650)
Exopalaemon carinicauda EC09
z
F:CGAGCCACAAAAGACACAT
R:CAAAGACGTGGACGAAATT
(TCT)10 147–190 30 4 0.5667 (0.6469)
Exopalaemon carinicauda EC10
z
F:AATCCTTCGGGAATCTAGTC
R:CAGAATAGCACCTTTCCAGA
(TGAT)5 404–489 30 9 0.5333 (0.8107)
Exopalaemon carinicauda EC11
z
F:TTTCCCAAGCCGTAACTA
R:AGGTGTGGAGACAGTGCC
(CA)6 110–172 30 3 0.3000* (0.4910)
Exopalaemon carinicauda EC12
z
F:TGGAATCCTCTCCTGCAT
R:CACTGATTGACTTTGGGC
(TA)6 298–361 30 11 0.8571 (0.9058)
Exopalaemon carinicauda EC13
z
F:CCTCTGGGCTGTAGGATGT
R:GGTACATGACAGGGTCCGT
(CT)28 242–305 30 5 0.6071 (0.6149)
Exopalaemon carinicauda EC14
z
F:GCAGCAGCACTTAGGTTAG
R:CTTGTGGAGCAAATAGGC
(AG)7 194–239 30 4 0.3571* (0.7188)
Exopalaemon carinicauda EC15
z
F:ATGGAGTCTCCGAGGATG
R:TGTAGGTGCTGGTGCTTG
(TC)10 103–148 30 6 0.6333 (0.5458)
Exopalaemon carinicauda EC16
z
F:ATGGAGTCTCCGAGGATG
R:TGTAGGTGCTGGTGCTTG
(CT)32 110–185 30 5 0.7143 (0.5669)
Exopalaemon carinicauda EC17
z
F:AAATCCGTGTCTGCTGCG
R:AGGAAAGCAAGGCTGGTG
(TACA)7 345–404 30 3 0.7333 (0.5893)
Exopalaemon carinicauda EC18
z
F:CAATGACGACCAGAACGAT
R:GAAAGCAACACTTGAAACG
(CTAT)8 190–242 30 4 0.4074 (0.7400)
Exopalaemon carinicauda EC19
z
F:AGGCTGGGTTGACAAATAC
R:CAAAGAGTGTGCAAAAAGG
(AGGGTT)5 242–340 30 4 0.8276 (0.7011)
Exopalaemon carinicauda EC20
z
F:CGTATCATTGTGTGCTTGTG
R:ACATCTACCTGGCGACATAT
(GGGTTA)5 89–110 30 5 0.4000 (0.6136)
Exopalaemon carinicauda EC21
z
F:GCATGTTGGCGTTTTGTAT
R:AAGGGGGTCTTATTTGAGC
(ACCCTA)11 187–236 30 6 0.5862 (0.6933)
Exopalaemon carinicauda EC22
z
F:CACGAGAACACTGTCCACTG
R:GCCCTGCTGTCTACATCCTT
(ATAA)5 180–213 30 4 0.7667 (0.6288)
Exopalaemon carinicauda EC23
z
F:GAATAGAGGAGGTGGCGTAG
R:TTTCACTTCTGAGGAGTAGGGAG
(AGT)16 275–324 30 3 0.4828* (0.4204)
Exopalaemon carinicauda EC24
z
F:AGGACATTGCGTCTGCTCA
R:CTGCCCTCATAATCCAGTGTC
(GGA)14 291–332 30 7 0.2500 (0.7981)
Exopalaemon carinicauda EC25
z
F:TGATGCAAGGAGAGCGTG
R:AAGGAATGCCTGCCTACTAT
(AAT)6 147–190 30 7 0.8667* (0.8164)
Exopalaemon carinicauda EC26
z
F:AGAAATACGCTATGGAAGACC
R:TTTACCAGCCCAATGAGT
(AT)7 353–401 30 7 0.9000 (0.8672)
Exopalaemon carinicauda EC27
z
F:ATCGGAACTCTCTAAATGCC
R:GTGTGCTGGTGCCTGAAA
(TC)28 146–187 30 11 0.7322 (0.9051)
Exopalaemon carinicauda EC28
z
F:GCGAAGTGAACATCAAAG
R:GGACAGAACCAGAAGCAG
(AG)20 345–405 30 10 0.9200 (0.8808)
Exopalaemon carinicauda EC29
z
F:GAACGCTGGTCAGAAACA
R:CTCATGGAAATCAATGTGGT
(AAG)7 242–267 30 10 0.9000 (0.8672)
Exopalaemon carinicauda EC30
z
F:TTCTCTCATTCGTTGGCATC
R:CAGAGAACATTGGCGAGAG
(TC)13 202–242 30 11 0.8667 (0.8949)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
119 120
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Exopalaemon carinicauda EC31
z
F:CTCCGAGGATGGACTAAAA
R:TAGGTGCTGGTGCCTTAAA
(CT)30 112–138 30 10 0.9667 (0.8712)
Exopalaemon carinicauda EC32
z
F:ATTGACGCCCATTGAGAT
R:TCATTTGTTGATGTCGGC
(CATA)5 231–267 30 8 0.7333 (0.8644)
Exopalaemon carinicauda EC33
z
F:ACCTTATGTCGCCAGTCC
R:CGCACCCATTACAGATTG
(GA)29 254–322 30 11 0.9333 (0.8706)
Lutjanus stellatus Ls01
A
F:GCCCCCAGCTAATTCTGTGTCT
R:GCCCAGTTTTTGTTGGTCTGAG
(GT)8 145 24 8 0.81 (0.79)
Lutjanus stellatus Ls03
A
F:CAGCTGCATGCGTCCTTTC
R:CAGATCCCCACAGCCTCCAGTGT
(CA)14 370 24 10 0.81 (0.71)
Lutjanus stellatus Ls04
A
F:TGTGACAAAGCGCCCTCCAG
R:TTCGCAAACAGATCCAAGACCTCA
(CA)9 298 24 21 0.94 (1.00)
Lutjanus stellatus Ls05
A
F:ATTCCAGGGCTGCAAAAATACTGT
R:CGGCCGCACTAGTGATTCTCTT
(AC)8 352 24 14 0.90 (0.71)
Lutjanus stellatus Ls06
A
F:AACCGCAAACTTTATCTCCACACT
R:TCTTTCTTGCCCTCTCCTCCTCA
(GA)9 392 24 11 0.87 (0.92)
Lutjanus stellatus Ls09
A
F:GAGGGGGAAAGGGAGGTCAT
R:TGAGTGAATGCCAAATTGAAGAAA
(TGTC)6 224 24 7 0.68 (0.58)
Lutjanus stellatus Ls10
A
F:CGTGCAGCCCAGTTTTTGTTT
R:CTGGGACGGGAGTGTTTTTGTAA
(GTT)5 275 24 10 0.88 (0.88)
Lutjanus stellatus Ls11
A
F:TCTCCGGTGTAAGTTCCTCCAGAA
R:CTGCGTCGTGTGATTGCTTTCA
(GT)8 276 24 8 0.73 (0.96)
Lutjanus stellatus Ls12
A
F:ATAACTGCAGGGCTCTGTGGTCTG
R:CAAAATTGCCAATGGGCTCTACAG
(TAT)5 316 24 16 0.93 (0.87)
Lutjanus stellatus Ls13
A
F:ACAACATGGCGCGTCAAAAC
R:GATCACAACATGTCTGCGAACTGT
(CA)9 200 24 11 0.83 (0.63)
Lutjanus stellatus Ls16
A
F:GATAGATCGCCGGTTTGATGAAT
R:TCACTCCAGTTTTCCATTTTGTCTT
(GATA)8 277 24 11 0.84 (0.96)
Lutjanus stellatus Ls17
A
F:AGCGCCAAGTCTCATCATCACAT
R:CACACCCAGCTCTCCACAAACAC
(CA)17 222 24 9 0.78 (0.63)
Lutjanus stellatus Ls19
A
F:CCAGGGCTTCAAAGCAAAGGTAG
R:AACAGATCCGCCGGTTGGTC
(GT)24 162 24 9 0.67 (0.66)
Lutjanus stellatus Ls20
A
F:TGAGAGGGTGATACAGAAAAACA
R:CAGACAGACGCACAAACATACA
(CT)43 247 24 7 0.83 (0.83)
Lutjanus stellatus Ls21
A
F:TGACTGTCCACCGGTAAGAGG
R:ACACTCGGTCCATCGTCGTC
(GT)17 271 24 5 0.88 (0.70)
Lutjanus stellatus Ls22
A
F:TTTGTCTGTCAGTGTTGTCTGTCC
R:TTTCCCCTCCAGCAAACTG
(GT)8 175 24 14 0.75 (0.84)
Lutjanus stellatus Ls23
A
F:AAACTGGCCTTTAATGACAGGA
R:TTATCTTTCCCCCATTTCTATCAC
(GT)10 242 24 9 0.75 (0.77)
Lutjanus stellatus Ls28
A
F:TGATTGCCAAGCTGAACATT
R:GCTCGAGGCTCATCAACAG
(CA)18 203 24 6 0.83 (0.80)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
121 122
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Lutjanus stellatus Ls30
A
F:GCTTACGCGTGGACTACTTTA
R:GTTTGTGCGATACTTTTCTTGAG
(CA)15 382 24 7 0.71 (0.75)
Lutjanus stellatus Ls31
A
F:GAGGGCTGGTGGGTATCTTT
R:TGCGTGTATGTGCAGGTCAT
(CA)13 333 24 8 0.79 (0.87)
Lutjanus stellatus Ls35
A
F:TGTGCAACAGGCTTTGAGATAC
R:CTGCCAGGGGTTTCACAAT
(GT)8 373 24 4 0.63 (0.69)
Lutjanus stellatus Ls37
A
F:GAGAGCGAGGAGGAGGAGA
R:ACTGTGTTCCCGTTGTGATG
(CA)12 315 24 7 0.83 (0.81)
Lutjanus stellatus Ls38
A
F:CACTGCCGCTACACTGC
R:TCTGAGCCTCTGTTTGAAGA
(CA)12 283 24 11 0.83 (0.91)
Sinopodophyllum hexandrum Sh8
B
F:CTCTTATCCATACTTAAATCC
R:GAGGATGCTGGAGGATGC
(TTA)5 193–199 65 4 0.519 (0.423)
Sinopodophyllum hexandrum Sh16
B
F:CGTGATGTAGGTGAGCTTAT
R:GTGAATCGTCTACTAGTGCA
(ATT)8 144–150 65 4 0.650 (0.409)
Sinopodophyllum hexandrum Sh33
B
F:CCAATAGTGAAGAATGACTTAA
R:GATGAAAACTTTGATTTGATGAT
(TCA)5 176–179 65 2 0.365 (0.320)
Sinopodophyllum hexandrum Sh42
B
F:GAACGCGAAGATGAAGAAGA
R:TTCAATCATCAGTAACAAACCA
(TGA)5 158–159 65 2 0.394 (0.231)
Sinopodophyllum hexandrum Sh45
B
F:GAACCCGCTAGGCTCAGT
R:GGATTCGTATTGATATACACAT
(CAA)5 160–172 65 6 0.733* (0.654)
Sinopodophyllum hexandrum Sh47
B
F:GGGGATGGTAAATGAATGCA
R:GAAAGGAAGGATCGAAAAGG
(TAT)6 165–168 65 2 0.473 (0.462)
Sinopodophyllum hexandrum Sh72
B
F:CCACAAACATAACATGCAAAC
R:CGATACCATCCTTATGGTAG
(CTG)5 167–170 65 2 0.440 (0.654)
Sinopodophyllum hexandrum Sh80
B
F:GATGATGGGATTTGGGAACT
R:GCCTAGCTAGCTCCCTTC
(ATG)8 194–197 65 2 0.482 (0.577)
Sinopodophyllum hexandrum Sh99
B
F:CCTGGTCGAGATGAAGAAAA
R:CTTGGCCAGATTTGCGCC
(CT)10 174–180 65 4 0.531 (0.560)
Sinopodophyllum hexandrum Sh121
B
F:CCTAGACGTGACAATGTTTTA
R:CAAGGCTTCGTGGAAGTTG
(TTTA)4 164–168 65 2 0.497 (0.462)
Sinopodophyllum hexandrum Sh131
B
F:GGGTGAACAAACCCTGTGT
R:CTATGTATTCGATGATGTTCG
(TTAA)7 170–175 65 2 0.500 (0.539)
Sinopodophyllum hexandrum Sh140
B
F:GGACCCAATTGTAGACACC
R:CTGTTGAGGCTGGTCCCT
(AG)8 192–215 65 2 0.426 (0.385)
Sinopodophyllum hexandrum Sh151
B
F:CCCGAGATGGGATCAAGC
R:GATGATTCGATTCTCTTTTCG
(TA)21 122–130 65 2 0.286 (0.192)
Sinopodophyllum hexandrum Sh161
B
F:GCCAATGACATGCAGATGTA
R:AGTAAGTAACTTCAATTAATAGG
(ATA)6 131–137 65 2 0.473 (0.231)
Sinopodophyllum hexandrum Sh163
B
F:GCCAGGCTCAGTTGCCC
R:CTCCCCTGCTGACCTCTA
(ACC)5 172–181 65 3 0.536 (0.462)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
123 124
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Sinopodophyllum hexandrum Sh164
B
F:GAATGGCAAATCCTAGTTACT
R:GCTTCACTTGCTTATTGACC
(AATT)3 181–186 65 4 0.598* (0.640)
Sinopodophyllum hexandrum Sh166
B
F:CCATTTATGTATAAAATTCCAGA
R:GAAATGTTCCAACACCACCA
(GAT)7 267–300 65 3 0.606 (0.600)
Sinopodophyllum hexandrum Sh168
B
F:CTGGAATATGTGCCATTGGT
R:CAGGAATTGCTTCGGTACG
(TTTA)3 149–153 65 4 0.748* (0.423)
Sinopodophyllum hexandrum Sh170
B
F:GGGATTCAATCACCGCCG
R:CGATCTCTCCGCAACCTC
(TTG)9 148–161 65 2 0.486 (0.583)
Sinopodophyllum hexandrum Sh175
B
F:CATTGGAAACGAGCTCTTTC
R:GTAAGTTAGCACCACAACTAA
(AGAA)4 159–162 65 2 0.499 (0.577)
Sinopodophyllum hexandrum Sh186
B
F:GTTGTCCTCTAGTGGGGTA
R:TAGCTTTACCAGCACCACC
(CTG)8 162–166 65 4 0.652* (0.654)
Sinopodophyllum hexandrum Sh209
B
F:GCAAGTCCGCCTCCAGG
R:GTGGGTCATATCACCTGCA
(TTA)4 158–161 65 2 0.493 (0.577)
Sinopodophyllum hexandrum Sh211
B
F:GTTGTCTGCCTTGATTATGG
R:GTAGGTTTGGTCAGAATTTTC
(GAT)8 152–156 65 3 0.642 (0.462)
Sinopodophyllum hexandrum Sh219
B
F:CTCTTATATGGATTTTAGGGC
R:CCCTACCACCGATGTGATA
(ATT)6 166–172 65 2 0.497 (0.600)
Sinopodophyllum hexandrum Sh225
B
F:CTACTGCTAACAATAATAGTCA
R:GAAGAGTCTAGCTACTCCG
(AAT)6 151–163 65 3 0.603 (0.615)
Sinopodophyllum hexandrum Sh236
B
F:GGATCATTGTCATAGCAACAA
R:CAAGGTGATCCTTTATCTGG
(TGA)9 141–146 65 5 0.743* (0.615)
Litopenaeus vannamei M6087
C
F:CTCTCCCTCTCTCCCTCTCC
R:TTTCCCGCGTAATCAGTACC
(CTA)5…(TTG)6 264–273 40 2 0.275 (0.276)
Litopenaeus vannamei M39565
C
F:TTTTTCTCTTCCTCCTCCGC
R:CCTCGACCCTTCAGTGTTGT
(TCC)5…(TCT)5 255–267 40 5 0.500 (0.488)
Litopenaeus vannamei M2758
C
F:TGTTTTGAATGCAGTGCCA
R:GAGGATTGAAGGCAAACTGC
(TTA)9 254–260 40 3 0.550 (0.538)
Litopenaeus vannamei M2553
C
F:ACAACTGCGCTGGAGAAGAT
R:TGGATAGAGAGAGAGCGGGA
(TAT)13…(TTA)5 151–157 40 3 0.550 (0.553)
Litopenaeus vannamei M12585
C
F:CATTAGGATAATGACAGTAATAAGGGT
R:TGATAATGGTAATGGTATTGATAATGA
(ATT)5…(ATT)5 143–172 40 3 0.750 (0.592)
Litopenaeus vannamei M2837
C
F:ACTATTCGACGCCTGAGTGG
R:AACAGCGAATCCAGAAGGAA
(AT)8…(AT)6…(CA)8 191–195 40 3 0.650 (0.649)
Litopenaeus vannamei M12607
C
F:AGCGTAGCACCTCTGCATTT
R:AAGAGGAAGACGAAGGAGGC
(CTC)6 196–206 40 4 0.825 (0.692)
Litopenaeus vannamei M13810
C
F:GACGGTGACTTGAAGGGTGT
R:TGCACCGTCTTTGTAATCCA
(AAG)6…(GGA)5 190–202 40 5 0.725 (0.727)
Litopenaeus vannamei M597
C
F:AAAAGAAAGCACACGAAAGG
R:CATCGGAGGCTGCAATTTAT
(CAA)9 174–189 40 6 0.718 (0.732)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
125 126
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Litopenaeus vannamei M13347
C
F:CCATTCTCAAGCCCATTGTT
R:GTTGATGGTGGTGGTTGATG
(TCA)5…(ATC)5…(ATC)5 203–218 40 6 0.850 (0.749)
Litopenaeus vannamei M56366
C
F:CCTTCAGACTCCAGCATTCC
R:AAACAAACAAACGGCCAAAC
(TTGT)5…(GTTT)5 208–221 40 6 0.667 (0.742)
Litopenaeus vannamei M15481
C
F:CCATTCTCATTCTCATTCTCTTTTT
R:AAGATGGCAATTCCAACAGC
(ATA)11 128–143 40 6 0.675 (0.758)
Litopenaeus vannamei M11263
C
F:CTTAATCTTGGAAGCCAGCG
R:CGAAAAACAAGAAGATACAAACGA
(TTG)6(GTG)8 152–179 40 7 0.825 (0.772)
Litopenaeus vannamei M24175
C
F:CAGCTTCCATTTCACGAACA
R:CAAAGAAGATGAAGACATAGGCAA
(TTC)5tttg(TCT)5 150–181 40 7 0.850 (0.785)
Litopenaeus vannamei M21670
C
F:TGCTATCATTACTACCACTGATACTGC
R:TCTCTCGACCCAGTGAAAGG
(ACT)5…(TAC)6 217–229 40 5 0.615* (0.788)
Litopenaeus vannamei M47106
C
F:ATGGGCACATGTTCTTAGCC
R:GAAAGTTCAAGGAAAGGGGG
(ATA)5t(TAA)6(A)12 236–258 40 7 0.750 (0.797)
Litopenaeus vannamei M1590
C
F:TCCCTTTGCATCTTGCTCTT
R:GAAGGTGAGGGAAGGGGTAG
(TCA)18 145–178 40 10 0.900 (0.804)
Litopenaeus vannamei M4150
C
F:ACCGGAAAAGATTGCAAAAA
R:GGCTGTATTCATTTTTGGGG
(T)10at(A)10(AT)8 194–221 40 6 0.590 (0.807)
Litopenaeus vannamei M6687
C
F:GAGTTGAAGAAGGAAATGTCGG
R:TTTCTCTTTGCCCATTGTCC
(ATG)8…(AGA)5 210–228 40 7 0.625* (0.816)
Litopenaeus vannamei M5476
C
F:GGCGAAGAAATAGCAACCAA
R:TTCGACACGCCTAGTACACG
(AAT)8 264–280 40 7 0.750 (0.826)
Litopenaeus vannamei M9990
C
F:CACGAAGAAGAAGACGAAGGA
R:AGCATAGGCGCTAGAACAGC
(AGA)6ta(AAG)6ata(AAG)6 230–263 40 13 0.875 (0.835)
Litopenaeus vannamei M28899
C
F:ATGGGAAAATGGAGGAAAGG
R:TGGTGGTTTTCCTGTAAGAAGG
(AAT)12…(G)11 266–290 40 7 0.625* (0.841)
Litopenaeus vannamei M11975
C
F:TTGTCATTGTTATTGTTATGACTGTTG
R:AACTAGGTCGGGGAAACACC
(CAT)13 118–163 40 9 0.825 (0.841)
Litopenaeus vannamei M17687
C
F:GTTTGGTGAGGTCACGCTCT
R:CGCAAGTTCTTCGCATTCTA
(T)12(TTA)5…(TTA)5 224–238 40 7 0.900 (0.841)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44

UNCORRECTED PROOF
127
Species Locus Primer sequence (50–30) Repeat motif Size
range (bp)
Sample
size (N)
Number of
alleles (N
A
)
H
o
(H
e
)
Litopenaeus vannamei M2882
C
F:CGACAGCTGTTGCTCAAGAC
R:AAAAGGTCCTTCGGGAGTGT
(CTC)5t(TCC)6 265–301 40 10 0.900 (0.827)
* Indicates significant deviations from Hardy–Weinberg expectations after Bonferroni corrections
#
Indicates fluorescently labelled M13(-21) primer (50-TGTAAAACGACGGCCAGT-30)
$
Indicates CAG tag (50-CAGTCGGGCGTCATCA-30) label
a
Wang et al. Development and characterization of 42 novel polymorphic microsatellite markers from Synechogobius hasta EST sequences (ESM 1)
b
Wu et al. Isolation and characterization of 22 nuclear microsatellite markers for Platevindex mortoni (Mollusca: Gastropoda:Eupulmonata) based on transcriptome sequencing (ESM 2)
c
He et al. Charazterization of 27 polymorphic microsatellite markers in the Onychostoma sima isolated by next-generation sequencing (ESM 3)
d
Ramos et al. Microsatellite markers for Euterpe precatoria Mart. (Arecaceae) a palm species used by extractive traditional farmers of Amazon (ESM 4)
e
Liang et al. Development and characterization of 36 novel microsatellite loci in Coilia nasus (ESM 5)
f
Bezerra and Gaiotto. Development and characterization of microsatellite markers for Chaetomys subspinosus (Rodentia: Erethizontidae), an endangered species, endemic to the Brazilian
Atlantic Rainforest (ESM 6)
g
Li et al. Development and characterization of 21 microsatellite markers for Spinibarbus caldwelli (Cyprinidae: Spinibarbus) (ESM 7)
h
Li et al. Development and characterization of 24 novel polymorphic microsatellites for the critically endangered plant Taihangia rupestris by transcriptome sequencing (ESM 8)
i
Moniek et al. Characterization of microsatellite markers in the hen harrier (Circus cyaneus) (ESM 9)
j
Zhu et al. Twenty-four novel tetranucleotide microsatellite markers for Epinephelus awoara (ESM 10)
k
Chen et al. Isolation and characterization of 28 polymorphic microsatellite markers of the Pacific seaweed pipefish Syngnathus schlegeli (ESM 11)
l
Gillet et al. Isolation, characterization and PCR multiplexing of polymorphic microsatellite markers in the endangered Pyrenean desman, Galemys pyrenaicus (ESM 12)
m
Li et al. Isolation and characterization of dinucleotide microsatellite repeat in genomic sequence of the orange-spotted grouper (Epinephelus coioides) (ESM 13)
n
Li et al. Development of transcriptome-derived microsatellite markers of the coral trout (Plectropomus leopardus) (ESM 14)
o
Contina et al. Genome-wide identification and characterization of simple sequence repeats in the painted bunting (Passerina ciris) for population and forensic analyses (ESM 15)
p
Viana et al. First set of polymorphic microsatellite markers for Brycon orbignyanus, an endangered Neotropical piracema fish species (ESM 16)
q
Li et al. Isolation and characterization of 36 novel EST-based microsatellite markers for blunt snout bream (Megalobrama amblycephala) based on transcriptome analysis (ESM 17)
r
Lam et al. Development of 24 polymorphic microsatellite loci for the threatened Southern (California) sea otter (Enhydra lutris nereis) (ESM 18)
s
Zhao et al. Development of 43 novel polymorphic microsatellite markers for Takifugu fasciatus and cross amplification in Takifugu xanthopterus (ESM 19)
t
Luo et al. Development of polymorphic microsatellite markers for an endangered fish, lined seahorse (Hippocampus erectus) (ESM 20)
u
Baerwald et al. Development of novel polymorphic microsatellite markers for two bat species affected by wind turbines, hoary bats (Lasiurus cinereus) and silver-haired bats (Lasionycteris
noctivagans) (ESM 21)
v
Liu et al. High-throughput microsatellite development of a migratory fish, Saurogobio dabryi Bleeker, 1871 for landscape ecological and genetic research in the Yangtze Basin (ESM 22)
w
Franc¸oso et al. Microsatellite loci for Tetrapedia diversipes (Hymenoptera: Apidae) (ESM 23)
x
Jiang et al. Fifty new microsatellite markers in the grand jacknife clam Solen grandis (Dunker) isolated via Hiseq 2000 sequencing (ESM 24)
y
Tang et al. Characterization of 30 novel polymorphic genomic microsatellite markers for Boehmeria longispica Steud (ESM 25)
z
Wang et al. Isolation and characterization of microsatellite markers in the ridgetail white prawn Exopalaemon carinicauda (ESM 26)
A
Wen et al. Characterization of 23 polymorphic microsatellites in a marine fish white-spotted snapper (Lutjanus stellatus) (ESM 27)
B
Fu et al. Development of 26 nuclear microsatellites for red-listed endangered herb Sinopodophyllum hexandrum by next-generation sequencing (ESM 28)
C
Yu et al. Characterization of 25 microsatellites for Pacific white shrimp Litopeneaus vannamei (ESM 29)
Conservation Genet Resour
123
Journal : Large 12686 Dispatch : 7-3-2016 Pages : 44
Article No. : 522 hLE hTYPESET
MS Code : Microsatellite letters vol 8 no 1 hCP hDISK
44