No full-text available
To read the full-text of this research,
you can request a copy directly from the authors.
The effect of laser radiation on Leydig cells: Functional and morphological studies
Abstract
The aim of the present study was to investigate whether Mid-Laser radiation could affect the functional and/or morphological characteristics of mouse testes. Mouse testes were exposed 'in vivo' to Mid-Laser radiation, and testosterone (T) production by the Laser-irradiated Leydig cells was assessed 'in vitro'. Leydig cells obtained from non-irradiated testes were employed as controls. In addition, both Laser-irradiated and control testes were studied under the light and electron microscope. 'In vitro' studies have shown a significant increase in the basal and LH-stimulated (0.148 to 0.500 mIU) T production by Leydig cell preparations obtained from the Laser-irradiated testes (P < 0.01 vs. control values). In contrast, morphological studies have failed to show any differences between Laser-irradiated and control testes. The results indicate that Mid-laser irradiation may increase the steroidogenic activity of mouse testes, with no changes in their morphological features.
... In particular, M.D. Porras et al. (1986) [68] showed an increase in the number of spermatogonia and activation of spermatogenesis after the irradiation of mice testes with continuous infrared LILI. A significant increase in the production of testosterone by the interstitial cells of the testes of mice (Leydig cells) is also reported as a result of laser illumination by a red continuous LILI with a 633 nm wavelength [69][70][71]. ...
... In particular, M.D. Porras et al. (1986) [68] showed an increase in the number of spermatogonia and activation of spermatogenesis after the irradiation of mice testes with continuous infrared LILI. A significant increase in the production of testosterone by the interstitial cells of the testes of mice (Leydig cells) is also reported as a result of laser illumination by a red continuous LILI with a 633 nm wavelength [69][70][71]. ...
In half of the cases, the infertility of the couple is due to the disorder of the male fertility. The leading factors that cause male infertility are urogenital infections, disorders of the immune system, testicular and prostate pathology, as well as endocrine disorders.
Low level laser therapy (LLLT) is a very effective physical therapy method, used in many areas of medicine, including obstetrics and gynaecology, andrology and urology; and it is recommended as an integral part of the complex treatment of infertility.
The literature review showed that LLLT is beneficial in treating male infertility. Laser can significantly improve the survival, motility and speed of movement of spermatozoa. Laser therapy of patients with prostatitis and vesiculitis can eliminate infiltrative-exudative changes, improve reproductive and copulatory functions. Local illumination of red (635 nm) and infrared (904 nm) spectra should be combined with intravenous laser blood illumination (ILBI) of red (635 nm) and ultraviolet (UV) (365 nm) spectra.
... В частности, M.D. Porras et al. (1986) показали увеличение числа сперматогониев и активацию сперматогенеза после воздействия непрерывным ИК НИЛИ на яички. Также сообщается о значительном увеличении производства тестостерона интерстициальными клетками яичек мышей (клеток Лейдига) вследствие лазерного освечивания красным непрерывным НИЛИ с длиной волны 633 нм [42,43]. ...
Male infertility is a multifactorial syndrome, which includes a wide range of disorders. It is a symptom of many different pathological conditions affecting both the reproductive and other body systems: endocrine, nervous, blood-vascular, and immune systems. Low level laser therapy is a method of modern physiotherapy, in which the impact is carried out by low-intensity laser irradiation (LILI). It is widely used in all areas of modern medicine, due to its high efficiency, simplicity of use, the absence of contraindications and side effects. The results of russian and foreign experimental studies on the article subject were analyzed. A definite conclusion is drawn that low level laser therapy should be used as much as possible in the complex treatment of men with infertility, since the effectiveness of the method is not just high, but often has no alternatives. At the same time, the available low level laser therapy techniques should be widely used: locally, rectally, laser acupuncture, ILBI (intravenous laser blood irradiation), on the projection of various organs, paravertebrally, etc. All parameters of laser action should be set (wavelength; mode of operation; frequency for pulsed lasers; power; power density, determined by the method of exposure; exposure, localization), which are specified by the relevant regulatory documents and clinical recommendations. (For citation: Moskvin SV, Borovets SJu, Toropov VA. Experimental justification of laser therapy efficiency of men's infertility. Urologicheskie vedomosti. 2017;7(4):44-53. doi: 10.17816/uroved7444-53).
... Moreover, there are evidences that low-energy laser light stimulates both in vitro fertilization and calcium transport in mammalian sperm mitochondria [24,25]. Porras et al. [26], working with testicular cells, showed an increase in the number of spermatogonia and Celani et al. [27][28][29] reported a significant increase of testosterone production by mouse interstitial cells after laser irradiation. Bermudez et al. [3,4] reported an increase in the germ cell DNA content shortly after IR laser radiation and after one cycle of the seminiferous epithelium. ...
Low level laser radiation stimulates both nucleic acid synthesis and cellular proliferation in E. coli, Hela tumor cells, fibroblasts, lymphocytes, and thyroid cells. It has been introduced as a therapeutic modality; nevertheless few studies have been carried out to determine the effects of laser radiation on the testes or spermatogenesis. The aim of this study was to determine the quantitative and qualitative changes of the seminiferous epithelium after Ga. Al. As. (830 nm) laser radiation.
The left testes of Sprague-Dawley rats were daily exposed to laser light for 15 days; so the cumulative doses used 28.05 and 46.80 J/cm(2) in two experimental groups. Sampling carried out 24 hours after the last treatment and samples were processed for LM and TEM study.
The number of germ cells specially the pachytene spermatocytes and elongated spermatids increased after 28.05 J/cm(2) laser radiation. Ultrastructural features of germ and Sertoli cells in this group were similar to that of control; while laser irradiation at 46.80 J/cm(2) had a destructive effect on the seminiferous epithelium such as dissociation of immature spermatids and evident ultrastructural changes in them.
The findings confirmed the existence of a biostimulatory threshold of applied laser energy and the importance of determining it for clinical applications. Moreover, it was revealed that low doses of laser light have a biostimulatory effect on the spermatogenesis and may provide benefits to the patients with oligospermia and azoospermia.
ResearchGate has not been able to resolve any references for this publication.