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Background: A few factors such as age, stress, and emotions may lead to impaired learning, memory loss, amnesia, and dementia or threats like schizophrenia and Alzheimer's disease (AD). Iranian traditional medicine (ITM) recommends some herbs and herbal preparations for the treatment or prevention of CNS problems. Methods: In this study, scientific evidence related to the effectiveness of ITM herbal medicine on memory, learning and AD is reviewed. The scientific evidence of plant efficacy was searched in electronic databases including PubMed, Scopus, SID, Science Direct, and Google Scholar by keywords such as memory, Alzheimer, amnesia, learning and scientific plant names from 1969 to 2014. Results: The findings of this study confirmed the effectiveness of certain ITM medicinal plants on enhancing memory and learning or in the treatment/prevention of amnesia and AD. Some ITM plants like Melissa officinalis, Crocus sativus and Nigella sativa showed improving effects on memory and the treatment of AD in clinical trials. In some cases, active principles responsible for the efficacy of these plants on memory were also determined. Discussion: Most of the studies on ITM plants were designed in animal models and a few herbs were evaluated in clinical trials on AD. Furthermore, there are insufficient or no investigations on certain herbal medicines used in ITM to confirm their effectiveness on memory and learning. Therefore, further experimental and clinical studies are necessary to evaluate the effectiveness of these plants on memory and AD as well as determining their active components.
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Shojaii et al., Afr J Tradit Complement Altern Med. (2016) 13(2):199-209
Asie Shojaii1*, Roshanak Ghods2, Mehri abdollahi Fard1
1. Department of medicinal plant, Research Institute for Islamic and Complementary Medicine, Iran
University of Medical Sciences, Tehran, Iran. 2. Department of traditional medicine, Research Institute for
Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran.
*Corresponding Author E-mail:
Background: There are some factors such as age, stress and emotions that may lead to impaired learning, memory loss, amnesia,
and dementia or threats like Schizophrenia and Alzheimer's disease (AD). Traditional Iranian medicine (TIM) recommended some
herbs and herbal preparations for treatment or prevention of CNS problems. In this study, the scientific evidences related to
effectiveness of TIM herbal medicine on memory and learning will be reviewed.
Materials and Methods: The scientific evidences of the plant efficacy were searched in electronic databases including Pub Med,
Scopus, SID, Science Direct, and Google Scholar by keywords like memory, Alzheimer, amnesia, learning and scientific plant
names from 1969 to 2014.
Results: The finding of this study confirmed effectiveness of some of TIM medicinal plants on enhancing memory and learning or in
treatment or prevention of amnesia and AD. Some of TIM plants like Melissa officinalis, Crocus sativus and Nigella sativa showed
improving effect on memory in clinical trials. In some cases, the active principles responsible for efficacy of these plants on memory
were also determined.
Conclusion: Most of the studies on TIM plants were designed in animal models and a few herbs were evaluated in clinical trials on
AD. Also, for some of the herbal medicine used in TIM, there are no or not enough studies to confirm their effectiveness on memory
and learning. Therefore, further experimental and clinical studies are necessary to evaluate the effectiveness of these plants on
memory and AD and also to determine the active components of them.
Key words: Memory, Iranian Traditional Medicine, Medicinal plants, learning
Learning is the process of acquiring knowledge about the world, and memory is the retention of the acquired knowledge
which can be retrieved as and when required (Kupfermann, 1993). Memory is an important part of Cognition for which the brain
plays interesting games of neurotransmitter with billion of neurons. Different types of memory are associated with different parts of
the brain. For example, Short term memory is associated with prefrontal cortex, Long term memory occurs in hippocampus and
temporary lobe and skill memory processed in cerebellum (Terry et al., 2011). The disturbance in such area leads to amnesia and
hence memory loss (Soman et al., 2013). Poor learning abilities, impaired memory, lower retention and slow recall are the common
problems in stressful situations. Moreover, age, stress and emotions are conditions that may lead to impaired learning, memory loss,
amnesia, and dementia or to more ominous threats like Schizophrenia and AD (Francis et al., 1999) AD is a neurodegenerative
disease that is characterized by progressive memory loss and cognitive impairment and beta-amyloid (
of neural functions (Long and Dougherty, 2003; Selkoe, 1994).
Nowadays, it seems that pharmacotherapy use of psychoactive drugs which are not effective in all cases and exerts
numerous side effects (especially with long term administration) causes different problems (Vyawahare et al., 2007). On the other
hand, there are some medicinal herbs which recommended through different traditional medicine as memory enhancing agents or
treatment of amnesia. Some of these herbs like Hypericum perforatum, Ginkgo biloba or Ginseng showed significant effect in
experimental or clinical studies on memory and learning (Rai et al., 1991; Wesnes et al., 2000; Hasanein and Shahidi, 2011; Abd-
Elhady et al., 2013).
Traditional Iranian medicine (TIM) includes all the knowledge and practices used in diagnosis, prevention and elimination
in Persia from ancient times to present and its roots go back over 2000 years (Naseri, 2004). TIM has recommended some herbs and
herbal preparations for treatment or prevention of CNS problems like epilepsy, Parkinson and amnesia (called Nesyan in TIM). In
the previous review, we mentioned the most important plants in TIM for treatment of epilepsy (Abdollahi Fard and Shojaii, 2013). In
the present study, the most important herbal medicine mentioned for memory enhancing and treatment of amnesia will be reviewed
and also scientific evidences which confirm their effectiveness will be discussed.
Materials and Methods
In this paper, we reviewed the most important ancient herbal treatments which are effective on learning, memory or treatment
of amnesia and AD. To find these herbal medicines, the most famous TIM books like Makhzan, Tohfeh, Tebe Akbari, and Moalejate
Shojaii et al., Afr J Tradit Complement Altern Med. (2016) 13(2):199-209
Aghili were searched and then plant scientific names were identified using combinations of old and new sources. We considered
these medicinal herbs as the key words for the next step of our study (Arzani, 2005; Aghili Khorasani, 2009; Tonkaboni, 2007).
Then electronic databases including Pub Med, Scopus, SID, Science Direct, and Google Scholar were searched from 1966 to 2014
for each of the plants in TIM plus memory, Alzheimer, amnesia and learning as the keywords; the findings summarized as tables.
There are some medicinal plants which were used in TIM for treatment of "Nesyan" (means amnesia) or for enhancing memory
and learning. The results showed that there are some experimental and clinical trials to confirm the effectiveness of these plants on
memory and learning and treatment of amnesia or related disease like Alzheimer (Table 1& 2).
Acorus Calamus
Acorus calamus is recommended for enhancing memory in TIM. Evaluation of the effect of A.calamus on improvement of
learning and memory showed that oral and intraperitoneal administration of the extract in higher dose could have increase spatial
recognition and recalling the data (Naderi et al., 2010). In a study, the effect of a polyherbal formulation (Bramhi Ghrita) containing
A.calamus on learning and memory in rats was evaluated. This preparation caused significant reduction in transfer latency in
Elevated Plus Maze (EPM) and escape latency in Morris Water Maze (MWM) test and so it act as a memory enhancer formulation
(Achliya et al., 2004).
The effect of A.calamus extract and its active principle, a-asarone on memory impairment, revealed that co-administration
of rats with A.calamus and a-asarone could prevent the stress induced alterations significantly. So administration of A. calamus
extract and a-asarone prevent noise; this noise stress induced memory impairment (Sundaramahalingam et al., 2013).
Allium Sativum
Garlic is mentioned to be effective in treatment of amnesia in TIM. Evaluation of the acute and chronic effects of crude
garlic extract on learning and memory showed that long-term administration of crude garlic extract may improve learning and
memory in mice while the underlying mechanism of action may be attributed to the anti-Acetylcholine Esterase (AchE) activity and
anti-oxidant property of garlic (Mukherjee and Banerjee, 2013). Also, it showed that twelve week treatment with shallot or garlic
significantly prevented the learning and memory deficits induced by fructose-feeding (Jalal et al., 2011). Evaluation of the
mechanism of garlic effect on memory showed that garlic administration in rats affects brain serotonin (5- hydroxytryptamine) levels
and memory-enhancing effect of garlic may be due to increase in serotonin metabolism in rat brain (Haider et al., 2008).
Table 1: Traditional medicinal plants for memory and learning
Name of plant
Traditional name
Part used
in TIM
Preparation or compound used in
experimental study
Acorus calamus
Ethanol extract
Ethylacetate extract
poly herbal formulation
Allium sativum
Crude garlic Extract
Aged garlic extract
Cooked garlic extract
Anacyclus pyrethrum
Hydroalcoholic extract
Boswellia sp.
Gum resin
Gum resin
aqueous extract
Ethanolic extract
-boswellic acid
aqueous extract
Boswellic acid fraction
aqueous Extract
Ethylacetate fraction
N-butanol fraction
Cocos nucifera
Coconut water
Crocus sativus
Alcoholic extract
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Methanol/water extract
Herbal formulation
Cyperus rotundus
So'de Kofi
Ethanolic extract
Ferula asa-foetida
Gum resin
Aqueous extract
Lavandula sp. (Lamiaceae)
L. officinalis
Ethanolic extract
Aqueous extract
Essential oil
Odor(Part of aromatherapy)
Melissa officinalis
Hydroalcoholic extract
Ethanolic extract
Nigella sativa
Phyllantus embelica
Methanolic extract
Hydroalcoholic extract
Ruta graveolens
Methanolic extract
Santalum album
Alcoholic extract
Aqueous extract
Terminallia chebula
Seed powder
Teucrium polium
Hydroalcoholic extract
Aqueous extract
Vitis vinifera
Methanolic extract
Methanolic fraction
Viniferin glucoside
Seed polyphenolic extract
skin extract of black grape
Zingiber officinalis
Alcoholic extract
Hydroalcoholic extract
Essential oil
*Maveez is a dried fruit of some kinds of grape
There are lots of studies about the effect of aged garlic on memory and it's mechanism of action. Investigation of the
beneficial effects of fresh, aged, and cooked garlic extracts on memory in diabetic rats showed that fresh and cooked but not aged
garlic extract (AGE) increased memory in diabetic rats significantly (Sarkaki et al., 2013). Another studies showed that chronic
ingestion of this extract markedly improved the learning deficits of Senescence-accelerated mouse (SAM) and also prevented brain
atrophy and kept the brain size at the control level (Nishiyama et al., 1997; Moriguchi et al., 1996). AGE also improved learning and
memory deficits of SAM and showed an antiaging effect on the SAM (Moriguchi et al., 1997). Chauhan and Sandoval showed that
feeding of AGE prevented deterioration of hippocampal based memory tasks in mice and suggesting that AGE has a potential for
preventing AD progression (Chauhan and Sandoval, 2007). It was suggested that consumption of garlic may lead to i
aggregation and AGE may improve cognitive impairment against -induced neuronal deficit, and has a beneficial effects on AD
(Gupta et al., 2009; Jeong et al., 2013).
S-allylcysteine is a major thioallyl compound found in garlic extract. Investigations revealed that diet supplementation
with S-allylcysteine may reduce age-related learning disabilities and cognitive disorders in senescence-accelerated mice (Nishiyama
et al., 2001). Furthermore AGE and its active ingredients, S-allyl-L-cysteine can restrict several pathological cascades related to the
synaptic degeneration and neuroinflammatory pathways associated with AD (Ray et al., 2011) and AGE protects neuronal PC12
cells against Aβ (25-35) toxicity in a dose dependent manner (Griffin et al., 2000).
The inhibitory effects of Z-ajoene, major compound containing sulfur in oil-macerated garlic products, showed against
scopolamine-induced memory impairment in mice. This study suggested that Z-ajoene may act on the cholinergic system and on
memory impairment caused by excess activity of AChE (Yamada et al., 2004).
Anacyclus Pyrethrum
Anacyclus pyrethrum, called "Aghergherha" in TIM, has been recommended for improving memory in traditional medicine.
Pre-treatment with the extract of A. pyrethrum roots (250 and 500 mg/kg) showed antiepileptic effect and also showed protection
Shojaii et al., Afr J Tradit Complement Altern Med. (2016) 13(2):199-209
against cognitive impairment by decreasing oxidative stress and rho kinase ROCK II expression in pentylenetetrazol kindled mice
(Pahuja et al., 2013).
Boswellia Sp.
The gum resin of Boswellia species (commonly named oilbanum) recommended for enhancing memory and treatment of
amnesia in traditional texts. The effect of olibanum gum resin on memory and learning of rat newborns was investigated in
numerous studies. In a study by Rasoli et al., aqueous extract of B. carterri gum resin (especially in dose 0.05 g/kg) showed
significant effect on learning ability and especially in the strengthening and maintaining of memory in rat's newborns comparing to
control groups (Tavakkolifar et al., 2009; Rasoli et al., 2001). Also, consumption of oilbanum in lactation period (8 weeks) in female
rats has a good effect on learning and short-term memory of newborns which their mothers use of this gum during lactation (Vahidi
et al., 2003). Hosseini et al. showed that olibanum could prevent impaired learning and memory caused by methimazole-induced
hypothyroidism in adult rats (Hosseini et al., 2010).
Ethyl acetate and N-butanol fractions of B. carterii gum resin showed significant effects on enhancing the memory ability in
rats which was much significant with ethyl acetate fraction (Hosseinzadeh et al., 2010). Consumption of the aqueous extract of B.
serrata gum resin in rat mothers during lactation in two-month old rats showed significant increase in short-term memory and long-
term memory in experimental group as compared to control group (Hosseini SharisAbad et al., 2003). Evaluation of the effect of the
aqueous extract of B. papyrifera on learning and memory revealed that it can be an agent for facilitation of learning and memory
(Farshchi et al., 2010). Another study on B. papyrifera revealed that it can affect spatial memory retention irrespective of the
treatment period and also systemic administration of the boswellic acids fraction obtained from B. papyrifera, enhanced spatial
memory retention in a dose-dependent manner (Mahmoudi et al., 2011).
The dendritic systems are the functional core of neuronal collections as they signify most of the receptive surface of neurons
and their organization is essential for integration and transfer of information at the synaptic level (Sharifabad and Esfandiari 2007).
In a study using Boswellia-treated young rats prenatally, it was concluded that better learning and memory performance in the
offspring of the mothers who consumed olibanum during their pregnancy is related to an increase in the somal volume of
hippocampal neurons in cornu ammonis and also these rats showed more dendritic branches in CA3 (cornu ammonis) pyramidal
neurons (Sharifabad and Esfandiari 2007). Beta boswellic acid, the major component of Boswellia serrata gum resin, could enhance
neurite outgrowth and branching in hippocampal neurons. Also long-term administration of Boswellia resin can attenuate age-related
dendritic regression in the superior region of cornu ammonis pyramidal cells in rat hippocampus (Hosseini-Sharifabad and
Esfandiari, 2015).
Cocos Nucifera
Cocos nucifera (coconut) is recommended for improving and enhancing memory in TIM. It was reported that young
coconut water has a high concentration of a hormone estrogen - which helps to delay the onset of AD. This experiment on
removing both ovaries of white mice, as a model of women with menopause that are deficient in estrogen, revealed that the mice that
received the coconut water have less pathological symptoms than the ones that did not receive it. It is stated in her recent study that
young coconut water could significantly reverse some pathologies associated with AD (Chomchalow, 2013).
Crocus Sativus
Crocus sativus (Saffron) is an important TIM herb which is mentioned to enhance memory. There are numerous studies
about the effect of saffron and its active constituent's crocin, crocetin and safranal on learning, memory and AD. Investigation of the
effects of an alcohol extract of C. sativus on learning and memory suggested that saffron extract ameliorates the impairment effects
of ethanol on learning and memory processes, and possesses a sedative effect (Zhang et al., 1994). In another study showed that pre-
training treatment with C. sativus extract could significantly antagonized the scopolamine-induced performance deficits in the step-
through passive avoidance test (Pitsikas and Sakellaridis, 2006). It has been shown that C. sativus extract antagonized ethanol-
induced memory impairment in the passive avoidance task in the mouse (Sugiura et al., 1995a).
Evaluation of the effect of saffron in aged mice showed significant improvement in learning and memory in saffron-treated
mice, accompanied by reduced lipid peroxidation products, higher total brain antioxidant activity and reduced caspase-3 activity in
age groups of mice (Papandreou et al., 2011). Investigation of the cognitive enhancement effect of an herbal extract containing C.
sativus showed that this preparation can reduce the decline in spatial cognition, which might be due to its effects on reducing
plaque formation and ameliorating histopathology and ultra structure in hippocampus of mouse brain (Cong et al., 2012).
Crocin is one of the active constituents of saffron which has been reported to enhance memory (Pitsikas et al., 2007).
Saffron extract and crocin improved ethanol-induced impairments of learning behaviors in mice, and prevented inhibition of
hippocampal long-term potentiation induced by ethanol (Abe and Saito, 2000). Also, it reported that crocin (50 to 200 mg/kg) had
beneficial effect on improving of ethanol-induced memory retrieval deficit of mice in passive avoidance task (Sugiura et al.,
1995b). Saito showed that crocin improved learning deficits by attenuating the inhibitory effect of ethanol on long-term potentiating
generation, and it can be considered as a candidate for treatment of ethanol-induced central nervous system disorders (Saito, 2004).
Also, crocin can inhibit scopolamine-induced impairments of spatial learning and memory in rats (Ghadami and Pourmotabbed,
2009). It has been shown that Intracerebroventricular streptozotocin (STZ) can cause cognitive impairment in rats. Evaluation of the
effects of crocin on cognitive performance of these rats showed that crocin treatment improved cognitive performance and resulted
in a significant reduction in malondialdehyde levels and elevation in total thiol content and glutathione peroxidase activity
(Naghizadeh et al., 2013). Further studies revealed that crocin specifically antagonizes the inhibitory effect of ethanol on N-methyl-
d-aspartate (NMDA) receptor-mediated responses in hippocampal neurons (Abe et al., 1998). C. sativus extract and crocin have
potential effect in the treatment of neurodegenerative diseases such as AD (Khalili et al., 2009; Khalili and Hamzeh, 2010).
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Also, saffron extract and crocin, improve spatial cognitive abilities following chronic cerebral hypo-perfusion and these
effects may be related to the antioxidant effects of these compounds (Hosseinzadeh et al., 2012). Evaluation of the effect of C.
sativus stigma extract and its constituents, crocin and safranal, on memory and learning showed that saffron aqueous extract, crocin
and safranal did not have effect on intact memory but the saffron extract and crocin inhibited the hyoscine unpaired
acquisition/performance activity (Hosseinzadeh and Ziaei, 2006). A similar study supported previous findings and suggested crocin
involvement in the modulating storage and/or retrieval of information. Crocin also showed positive effects in preventing the
impairment of learning and oxidative stress damage induced by chronic stress (Ghadrdoost et al., 2011).
The effect of saffron on AD was studied in several studies. It was shown that the water: methanolic extract of C. sativus stigmas
inhibited Ab-peptide fibrillogenesis possibly due to the presence of trans-crocin-4 and its digentibiosyl moiety (Papandreou et al.,
Two clinical trials by Akhondzadeh et al. revealed the effectiveness of saffron in mild to moderate AD (AD). In one study, 46
patients with probable AD received saffron capsules (15 mg) twice daily. Results suggest that using saffron capsules can be
associated with a better outcome on cognitive functions compared to the placebo group in a 16-week study (Akhondzadeh et al.,
2010a). Another study on 54 adults with similar doses of saffron evaluated the efficacy of saffron compared with donepezil (10
mg/day) in treating mild to moderate AD in a 22-week trial. Results demonstrate similarity in cases with adverse effects except
vomiting, which occurred more frequently in the donepezil treated group (Akhondzadeh et al., 2010b). In a randomized double-blind
clinical trial, treatment of electroconvulsive therapy-induced memory impairment with a preparation including saffron in patients
with mood disorders caused improvement in impaired memory, especially one or two months after the last electroconvulsive
therapy session. However, it had no significant effects during and immediately after the electroconvulsive therapy period
(Akuchekian et al., 2012). (Table 2)
Cyperus Rotundus
Cyperus rotundus is used for improving of memory in TIM. Evaluation of the effect of ethanolic extract of Cyperus
rotundus root (100, 200 mg/kg) on midazolam induced acute memory loss revealed that this extract significantly decreases the
transfer latency in EPM which measures the increase in memory at the time of retrieval and therefore cyperus rotundus shows
significant nootropic activity on retrieval and non significant on consolidation (Soman. et al., 2013).
Also, investigation of the effect of C. rotundus tubers on learning and memory in the rat model of AD suggest that C.
rotundus tubers has some repairing effects on the memory and behavioral disorders produced by lesioning of the nucleus basalis of
Meynert in rats (Rabiei et al., 2013).
Ferula Asafoetida
Ferula species are endemic plants of Iran and recommended for nervous problems like epilepsy and amnesia in TIM. Oral
administration of Ferula asafoetida aqueous gum extract showed significant improvement in memory score at 400 mg/kg dose in
passive avoidance model and dose-dependent improvement of transfer latency in EPM model. Also, dose-dependent inhibition of
brain cholinesterase and significant improvement in antioxidant levels were also observed. So, Memory enhancing potential of F.
asafoetida can be attributed to AchE inhibiting and antioxidant properties (Vijayalakshmi et al., 2012).
Lavandula Sp.
Lavender is used is some CNS disease like epilepsy, seizure and amnesia in TIM. Investigation of the effect of Lanvandula
officinalis extract on memory, learning and nociception in rats showed lavender extract at doses 100, 200 and 400 mg/kg improved
learning and memory compared to the control group (Rabiei et al., 2014).
Efficacy of Lavender also studied in AD. Soheili et al. showed that aqueous extract of L. angustifolia improves the spatial
performance of AD in rat. In the next study, they concluded that the lavender extract at the doses of 100 and 200 mg/kg markedly
decreased the extent of Ab aggregates which is a major cause of cognitive dysfunction in AD (Soheili et al., 2012). Also,
investigating the protein profile of the effects of aqueous extract of L. angustifolia on spatial performance of AD in rats showed 80
new proteins that expressed and 104 protein that suppressed in control group which receive 200 mg/kg of lavender (Videira et al.,
2013). Chronic exposure to L. angustifolia and L. hybrida essential oils in rats subjected to scopolamine-induced dementia, showed
positive effect on memory formation and reverse spatial memory deficits induced by dysfunction (Hritcu et al., 2012).
Use of lavender essential oil as a part of an aromatherapy in 28 elderly people (17 of whom had AD), showed significant
improvement in personal orientation related to cognitive function after therapy. So, this aromatherapy was efficacious therapy for
dementia (Jimbo et al., 2009). In a cross-over experiment, lavender or lemon odor improved performance four weeks later in both
free recall and recognition of a word list. In the other experimental group of this study to evaluate the effect of these two odor cues in
three learning and memory tests it showed that no significant main effect was observed on recall performance as a result of having
lemon or lavender at either learning or test. Although lavender had some effect on problem solving, context-dependent retrieval was
seen only in free recall and spatial learning (Parker et al., 2001).
Mellisa Officinalis
Melissa officinalis (Lemon balm) is mentioned to improve memory and consciousness in TIM. Hydro-alcoholic extract of
M. officinalis showed no significant effect on learning in MWM task but increases short-term memory in lower dose (25 mg/kg),
although, it may have prevention effect on making short-term memory in higher dose (100 mg/kg) (Yosofi et al., 2011) Soodi et al.
reported that M. officinalis (200 mg/kg) could significantly enhance learning and memory of rats and significantly ameliorate
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scopolamine-induced learning deficit. It is suggested that inhibition of AChE activity by M. officinalis extract is the at least one of
the mechanisms contributed in memory (Soodi et al., 2014).
There are some clinical trials for evaluating the effect of M. officinalis on memory. In a controlled trial, the effect of M.
officinalis extract on patients with mild to moderate AD was investigated. The results showed that patients who received M.
officinalis extract experienced significant benefits in cognition after 16 weeks of treatment. The findings was emphasized by the
improvements seen in both the cognitive subscale of AD assessment scale and clinical dementia ratingsum of the boxes measures in
the Melissa group (Akhondzadeh et al., 2003). In another study the acute effects of M. officinalis extract was studied on cognition
and mood of healthy, young participants at 7-day intervals. The results showed improvement in accuracy of attention with dose 600
mg of M. officinalis and time- and dose-specific reductions in both Secondary memory and working memory factors. This study
suggests that the ingestion of single doses of M. officinalis can modulate both the mood and the cognitive performance of healthy
volunteers in a dose- and time-dependent manner (Kennedy et al., 2003; Kennedy et al., 2002).
Nigella Sativa
The seeds of Nigella sativa used for enhancing learning and memory in TIM. Evaluation of the effect of Nigella sativa oil
on the spatial memory performance of rats revealed that there was a significant decrease in the overall mean number of working
memory error in the Nigella oil-treated group and therefore N. sativa oil could enhance the learning and memory abilities of the rats
(Sahak et al., 2013).
In a clinical study, the effect of N. sativa on memory, attention and cognition in elderly volunteers was evaluated in two
groups of volunteers during 9 weeks. The results showed significant difference in the score of logical memory test-I and II, total
score of digit span, 30 min delayed-recall, percent score in Rey-Osterrieth complex figure test, time taken to complete letter
cancellation test, time taken in trail making test-A and test-B, score in part C of stroop test due to ingestion of N. sativa and there
were not statistically significant changes in any of the biochemical markers during the study period. So, it can be a good candidate
food for preventing alzheimer's disease (Bin Sayeed et al., 2013) (Table. 2)
Phyllanthus Emblica
Phyllanthus emblica or Embellica officinalis is recommended for enhancing learning and memory in TIM. Evaluation of
the memory enhancing effect of the methanolic extract of P. emblica fruits showed that it could significantly improve learning and
memory, prevented scopolamine and sodium nitrite induced experimental amnesia and may be a great potential in memory deficits
(Ashwlayan and Singh, 2011). In another study it revealed that embelica extract possesses memory enhancing, antioxidant and anti-
cholinesterase activity (Golechha et al., 2012). Vasudevan et al. showed that Anwala churna (Emblica officinalis Gaertn) caused a
dose-dependent improvement in memory scores of mice and also it reversed the amnesia induced by scopolamine and diazepam. So
it may prove to be a useful remedy for the management of AD (Vasudevan and Parle, 2007).
Ruta Graveolens
Ruta graveolens is mentioned to increase nous in TIM. Evaluation of the efficiency of R. graveolens and Peganum
harmala extract in management of neuroinflammatory insults and neuronal apoptosis characterizing AD showed that treatment of
AD-induced rats with these methanolic extract, significantly ameliorates the cholinergic dysfunction and inflammation-induced
neurodegeneration characterizing AD. These effects were achieved through the powerful anti-inflammatory activity and anti-
apoptotic effects of these extract (Salem et al., 2013).
Santalum Album
Santalum album wood has been suggested for enhancing memory in TIM. In a study by Azmathulla et al., aqueous extract
of S. album increase the level of acetyl cholinesterase helpful in the brain for storing the memory and so it has a memory enhancing
property in mice (Azmathulla et al., 2010). Memory enhancing effect of the alcoholic extract of S. album was studied in mice induce
with Aβ protein for memory loss. The results show that the S. album has the property of increased enzyme levels. So, S. album L.
has the memory enhancing property (Papaiah et al., 2010).
Terminallia Chebula
Terminallia chebula has been used for different illnesses in TIM. It is recommended for improving memory and mind and
also treatment of amnesia. The ripe fruit of T. chebula is regarded as a promoter of intellect and memory, and is believed to prolong
life (Manyam, 1999; Misra, 1998). The ripe fruit is reputed to retard the ageing process and to improve cognitive processes and so,
has benefits in AD (Manyam, 1999).
In a study by Park et al., oral administration of T.chebula seeds ( 100mg/Kg) for 7 days caused neuroprotective effect
against ischemic damage in hipocampal C1 regions of the gerbil (Park et al., 2011).
Teucrium Polium
Teucrium polium was mentioned to exhibit positive effects on memory in Iranian traditional texts. The cognitive
impairment which occurs in diabetes mellitus, in a study by Hasanein and Shahidi, showed that administration of T.polium for 30
days from the onset of diabetes improved passive avoidance learning and memory of control rats and alleviated the negative
influence of diabetes on learning and memory (Hasanein and Shahidi, 2012). The findings of another study to evaluate antiamnesic
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activity of lamiacea plants including T. polium L., salvia officinalis and Melissa officinalis revealed that T. polium was active dose-
dependently, in antiamnesic experiment and it had the highest inhibition in the anticholinesterase assay among other plants (Orhan
and Aslan, 2009).
Vitis Vinifera
"Maveez" is a type of dried grape fruits which is mentioned for improving learning in TIM. The effect of methanolic
extract of Vitis vinifera and methanolic fraction of the extract on memory and behavior mediated by monoamines was studied and
the results suggest that V. vinifera possess nootropic activity in view of its facilitator effect on retention of learned task (Kakad et al.,
2008) peptides have an important role in AD. In a study by Riviere et al., viniferin glycoside, which was extracted from stem of
V. vinifera, exhibited the strongest inhibitory activity of Aβ (25-35) aggregation in vitro. Also, viniferin glycoside inhibited Aβ
cytotoxicity and the non-covalent complex between viniferin glycoside and Aβ (Rivière et al., 2010; Richard et al., 2011).
In another study, administration of grape seed polyphenolic extract for five months in in vitro
and attenuates cognitive impairment and AD-related neuropathology in the brains of transgenic mice (Liu et al., 2011). The black
grape skin extract reduces the reactive oxygen species production, protects the cellular membrane from oxidative damage, and
consequently prevents DNA fragmentation and so; it may be used to ameliorate the progression of pathology in AD disease therapy
(Russo et al., 2003).
Zingiber Officinalis
The rhizomes of Zingiber officinalis (ginger) were used for improving memory in TIM. Evaluation of the effects of
alcoholic extract of ginger in rats showed that cognitive function and neurons density in hippocampus of rats receiving ginger extract
were improved while the brain infarct volume was decreased. Indeed, it reduced cognitive deficits induced by focal cerebral
ischemia (Wattanathorn et al., 2011).
3, 4-methylenedioxymethamphetamine (MDMA) is known to produce brain damage and spatial memory impairment.
Findings of a study by Mehdizadeh et al. revealed that Z. officinalis could protect brain damage in MDMD treated rats and reduced
learning memory deficits that were induced by MDMA (Mehdizadeh et al., 2012). In another study, the essential oil of ginger
produced a cognitive impairment, in the passive avoidance test. The effects of ginger essential oil may be dependent on an
antagonist action on the central muscarinic cholinergic system (Bezerra Felipe et al., 2008). Evaluation of the effectiveness of Z.
officinale in combination with ginkgo biloba extracts revealed that 1 mg/kg of this mixture improved escape learning in the water
maze test (Topic et al., 2002).
Poor learning abilities, impaired memory, lower retention and slow recall are the common problems in stressful situations.
In addition, age, stress and emotions are conditions that may lead to impaired learning, memory loss, amnesia, and dementia or AD.
AD is a neurodegenerative disease and characterized by loss of learning ability with ageing (Francis et al., 1999, Terry et al., 2011).
Herbal medicine has been used for improving learning ability and enhancing memory in different traditional medicines.
TIM suggests some plants as single herb or in form of preparations for improving memory and for amnesia (Aghili Khorasani, 2009;
Tonkaboni, 2007). In this article, the most important TIM herbal treatments which are effective on learning, memory or treatment of
AD and amnesia and also scientific evidences for their efficacy were reviewed.
For Some of these plants, such as saffron, Olibanum gum, Garlic, Lavender and lemon balm, there are several studies
which confirmed the memory enhancing ability and effectiveness of them on learning and memory. The efficacy of saffron, lemon
balm, lavender and Nigella sativa was confirmed in clinical studies too. For some herbs such as saffron, garlic or ginger, the major
chemical constituents which may responsible for their memory enhancing effect was also studied.
There are some other herbal medicines in TIM which have still not been investigated for their pharmacological effect on
memory, such as Pistacia vera (Fostogh), Pistacia lentiscus (Mastaki) and Urginea maritime (Esghil).
Medicinal herbs which reviewed in this article would be good candidates for future studies on memory or treatment of AD
and amnesia. Some of TIM herbs such as Ruta graveolens or Cocos nucifera need further studies to confirm their effectiveness on
memory. It is suggested that more experimental and clinical studies were designed to confirm effectiveness of TIM plants on
memory, learning and AD as well as determining their active principles and mechanisms of action.
This study was supported by the Research Institute for Islamic and Complementary Medicine (Iran University of Medical
Sciences). The authors express their thanks to the library staff that helped them with the study’s data collection.
Shojaii et al., Afr J Tradit Complement Altern Med. (2016) 13(2):199-209
Table 2: Clinical studies of TIM medicinal plants effective on memory
Out come
,double blind
15 mg cap saffron(twice
daily) or Donpezil(5mg
twice daily)
54 adults with mild
to moderate AD**
Saffron at this
dose was effective
similar to
) Akhondzade et
al., 2010b(
RCT (16week)
15 mg cap saffron(twice
daily) or placebo
46 patients with
mild to moderate
Saffron has
better outcome
than placebo
(Akhondzadeh et
al., 2010a)
double blind RCT
Herbal combination(9gr
twice daily) or placebo
Mood disorders
which candidate for
Improvement in
impaired memory
(Akuchekian et
al., 2012)
method with
washout period 28
Part of aromatherapy***
28 elderly
people(17 of whom
had AD)
improvement in
orientation related
to cognitive
(Jimbo et al.,
Cross-over study
Lavender or lemon odor
1****.Ninety first
year psychology
2. Forty-eight
retrieval in free
recall and spatial
( Parker et al.,
Double blind RCT
(4 months)
Mellia extract 60 drops/day
or placebo
42 patients with
mild to moderate
improvement in
cognition in
mellisa group
(Akhondzadeh et
al., 2003)
RCT, balanced
cross over
Single dose of 300, 600,
900 mg of mellisa or
Healthy participants
modulate the
mood and the
( Kennedy et al.,
2003; Kennedy et
al., 2002)
(9 weeks)
500 mg cap or placebo
40 Healthy elderly
difference in the
score of logical
memory test
(Bin Sayeed et al.,
*RCT= Randomized, placebo controlled trial
**AD= Alzhiemer's disease
*** aromatherapy contitunts: aroma of 0.04 mL lemon and 0.08 mL rosemary essential oil in the morning and to 0.08 mL lavender
and 0.04 mL orange essential oils in the evening
**** Two different experimental groups
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Supplementary resource (1)

... They suggest Nigella sativa improves cognition by exerting a moderate facilitatory effect on dopaminergic neurotransmission. Shojaii et al., (2016) provided scientific information on Traditional Iranian Medicine herbal preparations presenting the beneficial effects of Nigella sativa on improving memory and in the treatment of Alzheimer's disease in clinical trials ( Shojaii et al., 2016). ...
... They suggest Nigella sativa improves cognition by exerting a moderate facilitatory effect on dopaminergic neurotransmission. Shojaii et al., (2016) provided scientific information on Traditional Iranian Medicine herbal preparations presenting the beneficial effects of Nigella sativa on improving memory and in the treatment of Alzheimer's disease in clinical trials ( Shojaii et al., 2016). ...
... There are many medicinal herbs effective in enhancing memory and learning. Ruta graveolens L. (Rutaceae), which has significant therapeutic properties, is one of the medicinal herbs used in traditional medicine in Iran and some other countries (Shojaii et al. 2016). This herb is referred to as panacea due to its high therapeutic value include the treatment of anticular, ear, pharynx, neurological, pulmonary, digestive, renal, gynecological diseases, parasites excretion, pain relief, and treatment of spasm and inflammation (Asgarpanah and Khoshkam 2012;Ratheesh et al. 2013;Baharvand-Ahmadi et al. 2015;Javadi and Emami 2015). ...
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Context: There are numerous pharmacological activities for Ruta graveolens and its bioactive constituent, rutin, on learning and memory. Objective: This study aimed to examine the effect of R. graveolens and rutin on memory in rats. Materials and methods: In this study animals were treated with the hydroalcholic extract of R. graveolens and rutin by IP injection for 10 days. Behavioural and biochemical tests as well as HPLC analysis and antioxidant activity of extract have been evaluated. Results: R. graveolens extract and rutin significantly increased learning and improved spatial memory, as well as secondary latency; moreover, there were significant increases in the serum and brain antioxidant capacity as well as the level of TBARS in serum and brain tissues. Results also showed that R. graveolens has significant DPPH radical scavenging effect (IC50: 159.17 ± 1.56 μg/mL). The HPLC analysis of extract showed that caffeic acid (19.92 ± 0.01), rutin (40.15 ± 0.01), and apigenin (0.84 ± 0.01) mg/g of dry extract are the main components of the extract. Discussion and conclusion: Regarding the effects of R. graveolens extract and rutin on animal brain cells, memory function, and learning, additional studies, including clinical trials, might be beneficial in producing natural supplementary drugs from this herb.
... Hence, in continuous to previous studies on efficacy of herbal remedies in ITM, in the present study, topical herbal remedies for insomnia and also related experimental and clinical articles have been studied. [16,17] ...
... Most population of the world, especially in developing countries relies on traditional medicine (Soleymani et al., 2010;Shahrajabian et al., 2011;Soleymani et al., 2013;Yong and Shahrajabian, 2017;Shahrajabian et al., 2018;Soleymani and Shahrajabian, 2018;Ogbaji et al., 2018;Yong et al., 2018;Shahrajabian et al., 2019a,b). Iranian traditional medicine is one of the most famous and ancient forms of traditional medicine, whose earliest record can be traced back to 6,500 BC (Shojaii et al., 2016;Zeinalian et al., 2017). It is believed that traditional Chinese medicine (TCM) has a 3000-4000-year history, and TCM spread across Asia throughout the centuries (Shahrajabian et al., 2019c,d,e). ...
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Traditional Chinese and Iranian medicine are ancient medical and health care systems that still forms an important part of diagnosis and treatment systems in Iran, China and other parts of the world. The integration of these two systems and modern biomedicine is also an important issue. Iranian and Chinese traditional medicines recommended for health promotion and well-being. Chinese herbal drugs have been described by medieval Muslim medical scholars such as Tabari, Rhazes, Haly Abbas, Avicenna and Jurjani (1137 CE). Traditional Chinese and Iranian medicine play an important role in sustainable agriculture and food systems, it also offers a holistic approach to prevent diseases while making appropriate use of organic and herbal products especially growth by small-scale family farmers. Both Iranian and Chinese traditional medicines may help to balance temperature, and both can be health prevention and preservation. Traditional healing practices consist of Islamic religious text based practices, Islamic worship practices and folk healing practices, Islamic religious text based practices are included spiritual healing, medicinal herbs, applied therapy and dietary prescriptions. At present, herbal medications or related products in the global market are derived from Chinese herbs, Indian herbs, Iranian herbs, Arabic herbs, and Western herbs. Herbal remedies may also be classified into three categories, namely, modern herbs, theoretical herbs, and empirical herbs, in accordance with their nature/characteristics and the nature of current usage. Traditional Chinese and Iranian medicines are inexpensive and available to all and they also focus more on fitness than illness
... Hence, in continuation of previous review studies on medicinal plants of Iranian traditional medicine, in this article, all published studies about E. amoenum were reviewed. [11][12][13] ...
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ABSTRACT Echium amoenum Fisch. and Mey. (Boraginaceae) is a plant which is used widely in Iranian folk medicine, especially for anxiety and depression. In this study, published scientifc reports about the composition and pharmacological properties of this plant were reviewed. The electronic databases including Google Scholar, PubMed, ScienceDirect, Scopus, Web of Science, and Scientifc Information Database were searched from 1970 to May 2016 and the data were summarized.Effcacy of E. amoenum (especially petals of E. amoenum) was studied in different in vitro, in vivo, and clinical evaluations. Furthermore, some chemical compounds such as rosmarinic acid, echimidine, and cyanogenic glycosides were isolated from E. amoenum petals. According to the results, E. amoenum showed various biological activities such as antimicrobial, antiviral, antioxidant, antidiabetic, analgesic, immunomodulatory, and anxiolytic effects. Clinical studies on E. amoenum showed effectiveness of this plant in depression and anxiety disorders. More clinical trials are recommended for evaluating different benefcial effects of this plant in human models and synthesis of new drugs from the active ingredients of this plant in the future. Key words: Biological activities, clinical, depression, chemical compound, Echium amoenum, in vivo, in vitro
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Background: Alzheimer's disease is the most common cause of dementia, especially in elderlies. Its exact pathogenesis is not yet known and existing therapies do not have enough efficacy. Therefore, the use of complementary therapies such as traditional Persian medicine for improving memory function is increasing. Objective: The aim of this study is evaluating recommended natural products in important traditional Persian medicine textbooks. Methods: For this purpose, keywords such as "Nesyan"(ie: oblivion, amnesia), "Hafezeh"(ie: memory), "Zekr"(ie: mention) and "Faramoushi"(ie: forgetfulness( were used to search in recommended prescription in important traditional Persian medicine textbooks. Then, the most repeated plants were extracted from the prescription and evaluated for their pharmacological properties recently published articles cited in the scientific databases such as Google Scholar, PubMed, Medline, and Scopus. Results: 262 natural products were in 96 evaluated prescription, 40 natural products were more repeated. These products were introduced in the table. Besides, their neuroprotective effects, antioxidant effects, and anti-Alzheimer's disease effect were also included in the table. Conclusion: Based on these results, traditional Persian has introduced many treatments for Alzheimer's disease, which requires clinical studies to prove or disprove their efficacy and safety.
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High‐cholesterol diet (HCD) is correlated with Alzheimer’s disease (AD) and impairment of memory. This study investigated beneficial therapeutic effects of Dill tablet and Ocimum basilicum (Basil) aqueous extract on hypercholesterolemia‐induced cognitive deficits and oxidative stress in hippocampus tissues of rats. Hippocampal Aβ(1‐42) level was measured. The gene expression levels of superoxide dismutase and inducible‐nitric oxide synthase were determined in hippocampus. Cognitive functions were examined and oxidative status was evaluated in serum and hippocampus. Phytochemical properties and in vitro antioxidant activity of Basil extract were assessed. HCD significantly increased serum cholesterol, induced deposition of Aβ plaque, altered hippocampus morphology, and impaired memory function, whereas receiving Basil extract or Dill tablet increased antioxidant potency in serum and hippocampus and normalized HCD‐induced deleterious effects. Basil extract and Dill tablet may exhibit their beneficial effects in AD by lowering serum cholesterol and evoking antioxidant system in the brain. Practical applications Dill tablet and Basil aqueous extract lowered serum cholesterol in hypercholesterolemic animal models, therefore, they can be used as hypocholesterolemic agents. These edible herbs significantly retarded deposition of Aβ plaque and normalized hippocampal morphology, thus, they favorably protected hippocampus tissue from deleterious effects‐induced by hypercholesterolemia. Dill tablet and Basil aqueous extract also corrected oxide‐redox balance and normalized HCD‐induced oxidative stress to some extent and significantly improved impairments in learning and memory suggesting that these medicinal plants can be considered as surrogate therapeutic agents for the synthetic medicines in the treatment of AD and in postponement of its complications.
Lipid solubility and molecular size are the major limiting factors for any molecule to get absorbed across biological membrane systems. Formulation strategies that address these concerns are of enormous benefit in eliciting the intended response from a therapy. Liposomes and phytosomes are two very versatile lipid-based delivery systems that cater to a wide spectrum of therapeutic applications on account of their composition and structural properties. This chapter was conceptualized with an aim to present liposomes and phytosomes on a common interface with special focus on their implications in CNS disorders besides several other applications. It presents a detailed account on their classification, composition, salient properties, and advantages, also describing the basic differences between them. Further the methods of preparation for liposomes and phytosomes along with their clinical and therapeutic applications have been discussed in great detail. Neurological diseases impose a huge burden of illness and have shown an increased incidence due to an increase in the average life expectancy in the global scenario. We have reviewed recent advances in the liposomal and phytosomal interventions that have been investigated for various neurological disorders.
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Nutraceuticals had always been known for their therapeutic effects in ancient medicine and had been the primary healing remedy until the introduction of modern chemistry and pharmacology. However, their use has not been dismissed but actually is acquiring a new acclamation among the scientific community especially for their efficacy on the Central Nervous System (CNS). Molecular mechanisms of the most common neurodegenerative diseases are now being uncovered and along with that the molecules that drive the neurodegenerative processes. It is not surprising that some natural compounds can interact with those molecules and interfere with the pathological pathways halting the cascades that ultimately lead to neuronal cell death. The plant Ruta graveolens has gained increased attention in medicinal chemistry due to its beneficial role to treat a variety of human diseases and also because of the presence of a huge number of compounds belonging to different classes of natural products, including neuroactive compounds potentially able to promote neuroprotection. Among all the components of the plant extract, rutin – which is highly, if not the most, abundant – positively interacts with the neurophysiology of the CNS too, being particularly efficient against neurotoxicity. Rutin, has proven to be protective in a variety of experimental settings of neurodegeneration. Finally, it has been shown that the water extract of Ruta graveolens (RGWE) induces death of glioblastoma cells but not of neuronal cells. Moreover, it also fosters cell cycle re-entry and differentiation of neuronal cells. This peculiarity represents a promising tool to promote neural plasticity in pathological conditions.
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We conducted a double-blind randomized placebo controlled trial to evaluate the impact of Lemon balm (Melissa officinalis L.) on the bleeding and systemic manifestations of menstruation. A total of 90 students were randomly assigned to treatment or placebo group. Bleeding and systemic manifestations were evaluated with a menstrual pictogram and multidimensional verbal scale before and during 2 consecutive menstrual cycles, respectively. Statistical tests indicated that in both groups, the severity of the systemic symptoms associated with dysmenorrhea significantly decreased (P = 0.001). Among the systemic symptoms, the mean severity of fatigue, the lethargy, and nervous changes in the two groups decreased after the treatment, which was statistically significant. There was no significant difference between the two groups regarding fatigue in the three cycles, but there was a significant difference between the two groups regarding lethargy in the first cycle (P = 0.05) and the second cycle (P = 0.001) after the treatment. The present study demonstrated that Melissa officinalis decreases the severity of the systemic signs associated with menstruation. It showed that the herb does not increase the severity of bleeding and the duration of menstruation. However, it reduces the mean total score of the severity of all the systemic symptoms associated with dysmenorrhea.
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The plant Santalum album Linn. is commonly known as Chandana (Family: Santalaceae) and whole plant is having medicinal active constituents, such as Carbohydrates, Triterpenoids, Volatile oils, α and β santalol etc are responsible for its activity. The aqueous extract of Santalum album onmemory enhancing activity was evaluated by using experimental model such as Amyloid-β peptide deposition in mice. The mice were treated with graded dose of Santalum album extract. The dose grading from 100mg/Kg to 500mg/Kg were administered orally to the mice and Donepezil was used as standard drug 0.5ml for the group I and VII i.e., dementia mice treated with Donepezil. The aqueous extract of Santalum album increase the level of Acetyl cholinesterase helpful in the brain for storing the memory, decreased levels of GSH , due to excess release of Glutathione may cause the excitotoxity and decreased the formation of reactive oxygen species due to its anti oxidant activity. Hence, it is concluded that, aqueous extract of Santalum album increases Memory Enhancing Property in mice.
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Background: Learning and memory impairment during aging mind have a disturbance role in human life. Regarding to the defect mechanisms for prevention or treatment of memory loss, the new strategies were recommended for study on it. Objective: Importance of herbal medicine treatment encouraged us to examine the effect of a candidate plant Acorus calamus for improvement of learning and memory. Methods: Male rats were randomly divided to control and treatment groups. In treatment group the plant were applied to animals in oral (plant/ food ratio = % 6.25) for two weeks and in injected groups the plant extract were applied (i.p) to rat in 25, 50 and 100 mg/kg). Finally, all experimental animal groups were conducted to Y maze and shuttle box tests in order to obtain spatial recognition and acquisition - recalling data, respectively. Results: The step through latency (STL) time resulted from oral and 100 mg/kg injected dose groups show a significant difference with control animals. Also, alternation behavior (%) obtained from Y maze test data was marked than control rats. Conclusion: The present study show that oral and intraperitoneal administration of the extract in higher dose could have increase spatial recognition and recalling the data,.
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The objective of the work was to investigate the effects of the essential oil of Zingiber officinale Roscoe (EOG - 25, 50 and 100 mg/kg, i.p) on the behavior and cognition of male Swiss mice (25 - 30 g), treated daily for 7 days. In the 7(th) day, animals were submitted to the elevated plus maze, open field and rota rod tests to evaluate anxiolytic, sedative and motor coordination effects, respectively. Memory tasks and cholinergic system effects of EOG were also assessed by the T-maze, passive avoidance, oxotremorine and pilocarpine-induced convulsions tests. EOG did not show anxiolytic effects in the elevated plus maze test, while it showed a sedative effect in the open field test at the doses of 50 and 100 mg/kg. EOG did not alter motor coordination of the animals, while it produced a cognitive impairment, in the passive avoidance test, in animals treated with the dose of 100 mg/kg. In the T-maze test, cognitive impairment was also evident and occurred in a dose-dependent manner. In addition, EOG (50 and 100 mg/kg, i.p.) blocked the oxotremorine-induced tremors and significantly increased the latency of pilocarpine-induced seizures, as well as animals survival. Altogether, our results suggest that EOG effects are, at least in part, dependent upon an antagonist action on the central muscarinic cholinergic system.
Alzheimer's disease (AD) is the most common cause of progressive intellectual failure and memory impairment among the elderly, affecting over 4 million Americans. A hallmark of the disease is the accumulation of β-amyloid, whose neurotoxicity has been well documented. A therapeutic approach may be to ameliorate this toxicity. Garlic has antioxidant, anti-aging and anti-ischemic properties. The purpose of the present study was to determine whether aged garlic extract attenuates the effect of neurotoxin, β-amyloid 25-35 (Aβ) in neuronal PC12 cells. Proliferating (non-neuronal) PC12 cells were treated with the nerve growth fact or for at leas 14 days before experiments were initiated. This treatment differentiates PC12 cells to a neuronal cell line. Cell viability was determined using the MTS-based assay (Promega). Wakunaga of America (Mission Viejo, CA) kindly donated aged garlic extract™ (AGE™) as Kyolic® liquid. AGE™ was sterilized by filtration and diluted in cell growth medium. Our results show that AGE™ protects neuronal PC12 cells against Aβ (95 nM) toxicity in a dose dependent manner. AGE™ may be effective in ameliorating Aβ-neurotoxicity.
Frankincense, also known as olibanum, is a gum resin collected from trees of the genus Boswellia. The gum resin is produced by trees of various Boswellia species. These plants are middle size trees from Burseraceae family. Commertial olibanum in Iran is obtained from Boswellia carteri and Boswellia serrata that are native of Africa and India, respectively. Boswellic acids (BAs), a group of pentacyclic triterpenic acids are main bioactive principles of the gum resins. Gum olibanum has a long history of use, for example in religious ceremonies and for perfume production. Also it has been traditionally used in the ayurvedic system of medicine as an anticancer, antidiahhroea, memory and learning enhancing. Recently, the investigations demonstrated that gum olibanum has pharmacological properties and clinical effectiveness in various diseases. Present review focuses on pharmacological activities of gum olibanum.
The mysterious organ called Brain receives information through five senses and interprets it and store it in the form of memory. Various pats and lobes are for different functions. Different areas of the brain are responsible for different types of memory. In such case by taking Indian medicinal system into consideration the current work performed to examined the effect of Cyperus rotundus roots ethanolic extract on Midazolam induced acute memory loss. Evidence concerning states that Midazolam can be used postoperatively to forget the pain, in the dose of 2 mg/kg Midazolam can induce acute memory loss and cyperus rotundus is traditionally used as a memory enhancer. In performed work by using Elevated plus maze apparatus for mice the Transfer latency was measured at the time of acquisition, consolidation and retrieval in mice in arranged groups. It was found that the ethanolic extract of cyperus rotundus significantly decreases the Transfer latency in Elevated plus maze for mice which measures the increase in memory in at the time of retrieval. The goal of the study is use of cyperus rotundus alternatively to the allopathic medicines as a memory enhancer but it requires more work to understand the responsible chemical constituent and mechanism of action for further development of the drug.
Background: In traditional medicine, a gum resin obtained from Boswellia carterii, has been used as an agent for enhancing memory and learning abilities. Objective: In the present research, the effect of fractions obtained from gum resin of Boswellia carterii was assessed on enhancement of memory in intact and on memory impairments induced in rats by hyoscine using the Morris water maze task. Methods: The ethyl acetate (0.1 mg/kg) and N-butanol (0.1 mg/kg) fractions were injected intraperitoneally to rats 1 h before training for 5 consecutive days. During the training period, four trials were carried out each day. On the 5th day of the experiment, the locomotor activity was assessed using open field test. The effect of ethyl acetate fractions was evaluated on memory impairment induced by hyoscine (0.5 mg/kg). Results: The ethyl acetate (0.1 mg/kg) and N-butanol (0.1 mg/kg) fractions showed significant effects in enhancing the memory ability in intact rats. This effect was much significant with ethyl acetate fraction (p<0.001). The ethyl acetate (0.1 mg/kg) fraction reduced the deficit effect of hyoscine on memory (p<0.001). The N-butanol and ethyl acetate fractions (0.1 g/kg) have no effect on locomotor activity. Conclusion: On the basis of these results, B. carterii gum resin improved intact memory and the hyoscine impaired acquisition/performance activity. This effect may be mediated via cholinergic system.
Background: The electrophysiological and behavioral studies have shown that saffron extract improves memory and learning skills in ethanol-induced learning behavior impairments. Objective: The aim of the present study was to evaluate the possible effects of Crocus sativus aqueous extract stigma and its constituents, safranal and crocin, on intact memory and scopolamine-induced learning deficits in rats performing the Morris water maze task. Method: The extract and its constituents were injected intraperitoneally to rats one h before training for five consecutive days. During the training period, four trials were carried out each day. On the fifth day of the experiment, the locomotor activity was assessed using open field test. Results: Generally, the aqueous extract (0.0025-0.56 g/kg) of stigma did not decrease the latency time to find the platform. Only a dose of 0.005 g/kg reduced the latency time during five days. Crocin (50 and 200 mg/kg) also did not change the latency time. Safranal (0.2-0.75 ml/kg) increased the time to find the platform. The effects of the extract, crocin and safranal on hyoscine-induced learning deficits were also evaluated (1 and 500 mg/kg hyoscine). The stigma extract and crocin reduced the deficit effect of hyoscine (in low and high dose) on memory. Safranal (except a dose of 0.2 ml/kg) did not decrease the effect of hyoscine on memory. In the open field activity test, the aqueous extract, crocin and safranal reduced total activity. The co-administration of these agents with hyocine significantly compensated the reduction of locomotor activity in hyosine group alone. Conclusion: On the basis of these results, the Crocus sativus stigma aqueous extract, crocin and safranal did not have effect on intact memory but the extract and crocin inhibited the hyoscine unpaired acquisition/performance activity. These agents can be potentially introduced as new drugs in the treatment of cognitive dysfunctions such as Alzheimer's disease.
Objective(s): Learning is defined as the acquisition of information and skills, while subsequent retention of that information is called memory. The objective of the present study was to investigate the effect of aqueous extract of Boswellia papyrifera on learning and memory paradigms in mice and rats. Materials and Methods: This study was held at the Department of Pharmacology, Faculty of Pharmacy, Kermanshah University of Medical Science, Kermanshah, Iran from September 2006 to March 2008. Male Wistar rats and male NMRI mice were randomly divided into control, B. papyrifera treated (50, 100, 150 mg/kg, p.o.), and piracetam (150 mg/kg) groups. Radial arm maze (RAM) and Morris water maze (MWM) were the screening tests used to assess the activity of B. papyrifera extract. Results: The mice treated with B. papyrifera (50, 100 and 150 mg/kg) or piracetam (150 mg/kg) showed a decrease in number of days required to learned (P< 0.05) and time taken to find food by the learned mice in radial arm maze (P< 0.01). In Morris water maze, rats treated with the above mentioned doses showed dose dependent improvement in spatial learning. Escape latency during swimming in water maze in piracetam and B. papyrifera treated animals was significantly lower (P< 0.01) than control. Swimming distance was also significantly lower (P< 0.05) in the treated groups. Conclusion: The results show facilitation of spatial learning and memory processes and thereby validate B. papyrifera traditional use of intelligence improving. The presence of alkaloids, flavonoids and saponins might be responsible for this activity of B. papyrifera.
Objective: The current study was planned to investigate anti-inflammatory and anti-apoptotic effects of Ruta graveolens and Pegenum harmala total extract against neurodegeneration characterizing Alzheimer's disease (AD) in rat model. Methods: Adult male Sprague Dawley rats were classified into five groups. Group (1) control group; group (2) AD group, was administered AlCl3 daily for one month; group (3) AD group treated orally with rivastigmine daily for three months; group (4) AD group divided into two subgroups, the first was treated orally with R. graveolens (750 mg/kg b.wt.) and the second was treated with R. graveolens (375 mg/kg b.wt.) daily for three months and group (5), AD group divided into two subgroups the first was treated orally with P. harmala (375 mg/kg b.wt.) and the second was treated with P. harmala (187.5 mg/kg b.wt.) daily for three months. Brain and serum acetycholinesterase (AchE) activity, monocyte chemotactic protein-1 (MCP-1), leukotriene B4 (LTB4) and B-cell lymphoma 2 (Bcl-2) levels were detected. Brain histological investigation of all studied groups was carried out. Results: It showed that AlCl3 administration resulted in significant elevation in brain and serum AchE, MCP-1 and LTB4 levels accompanied with significant depletion in brain and serum Bcl-2 level. Brain histological investigation of rats administered AlCl3 showed appearance of Aβ plaques characterizing AD. Treatment of rats with the selected extracts caused marked improvement in the measured biochemical parameters as well as in the histological feature of the brain. Conclusion: R. graveolens and P. harmala possess anti-inflammatory and anti-apoptotic effect against neuroinflammation characterizing AD.